MD14G1235100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010425
Physical Location & Seq
Forward (+)
31509034 .. 31515791
6758 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1235100.v1.1.491

Sequence Viewer

Length: 3087 bp
ATGAAGAACCTTCTTAAGAAACTGCATATCATGTCCAACCAATCAGAAGATTCGGAAGGGTCTGCAGCTTCATCAAGGGGCCGAAAGTCTATTAGCAAGTCATCCCCCGAAACCGAAAGGCTTTTGCACTCGAGGTCCCATCAGGGTTCTGAGCATAAACCCTTCTCGGGGATTTCGGGTTGGTTGAATTCAGTTGCCAATAAGCATGGCCCTAGTCCCCCATCATCTTCGAATGTCAACAGGGCAGCGAGAGTGGAGCAGCCACCTGATGCAGCTGTTAGTGGGAGTGGTTTAGATGTTGTTTCGGATACAGGTAGGCGCGATTCGGGGTCTAGTACCTCGAGGGATGCTGATATAGCAGAAGAGTATCAGATTCAATTGGCTTTGGAATTGAGTGCTAGGGAGGATCCAGAGGCAGTTCAGATTGAGGCTGTTAAGCAGATTAGCTTGGGGTCTTGTGCTCCTGATAATACTCCAGCTGAAGTTATAGCATACCGCTATTGGAATTACAATGCTCTTAGCTATGATGACAAGGTCATGGATGGTTTCTATGATCTGTATGGAATATTGACGGAGTCAACCTCCGAAAGAATGCCTTCCCTTGTTGATCTGCAAGGAACACCTTTGTCAGATAGTGTCACCTGGGAAGCAGTTTTGGTCAATAGAGCTGCCGATGCGAAGTTGTTGAAACTTGAACTGGTGGCCCTAGAAATGGCTGTAAAGTCAAGTTCAGATCCTCTGGCTTTTCTAAACAGCAATCTGGTGCGAAAACTTGCTCTTTTAGTTGCTGACCATATGGGTGGACCAGTTGCGAATCCTGAAAACATGTTGAGAGAATGGCAAAATCTTAGTTACAGTTTGAAAGGAACCATTGGTAGCATGGTTTTGCCACTTGGTTCTCTGACGATTGGATTAGCTCGACATCGTGCATTGTTGTTCAAGGTTTTGGCTGATAGTGTGAGCATCCCATGCCGGTTGGTGAAAGGACAACAATACACCGGGTCCAGTGATGTGGCAATGAACTTTGTAAAAGTTGATGATAGGGAGTATATTGTTGATCTGATGGCAGACCCTGGCACACTTATTCCATCTGATGCAGCTGGATCACATATAGAATATGATGAATCTTACTTTTCTGCCAGTCCTTTGTCTAGAGACATTGATTCCTCTCATGTTGCTTCTTCCAGTAGTGGAGTTGGGAGTTCGTTTGAAGAACATTCAGACTTTGGTACATTAGAGAAGAAATCCAGGTTAAGGAATTTTGCTTCTTCCACAAGGGATTCCGAAGAGAGAGAAGAACCAGCTTCTCGTGATCTACCCAGACCAACTGAATTCGAGGAACAACCCAAGATGCCTTCAGATGAATTTAGATATGCCTCTGACGTAGAAAAGATGCTGGTGCAGGAACTTCCAGAAAAGCCAAATTATCCTTTTGCGCATACGAGATCCCCTTCATGGACTGAAGGTGTTAGATCTCCAGCCGTTAATAAAAAGCAGGTTAAGGATGTTTCTAAATACATGATTGTTGCTGCCAAGGAGAATCCAAATCTAGCTCAAAAGCTTCATGATGTTTTACGTGAGAGTGGTGTTGTTGCTCCCCCGAACTTGTTTAGGGAAATATATCCAGAGGAGTTAGATGTGTCAACAGTCGAGACCAAACGCCGGGCAGAAGATAAGAACGAAAATAAAGAAAGGTTTGGAACACAAAAATTTAAAAGTCAAGATGATAAAAGGCCAGCTCACTTTCTGCCTCCTTTGCCTCAGCAAAGGGTGAATCTTAAAGCTAGTACTTCCGGTCAACCGGAGATTCTTAAGCCTGTGGAAGGTTTAGGCGTCAACCTTACACTTGATACGAGGGACGTAACTGGCCAGAATATCTCATCACAGTCCGAAGCATCTCCAGTTAAATATACAAATAATGTCCCTGTTGCTGCAGCGGCGGCGGCCGCAGCAGCTGTTGTTGCATCATCTATGGTAGTCGCTGCAGCAAAAACAAGTACTGACACAAGTCTCGAACTTCCTGTGGCAGCTGCTGCCACTGCTACTGCTGCAGCTGTGGTTGCAACAACCGTAGCTGTCAGTAAGCAATATGAGCCGGGCATGAGAAGTGATGGAGATTCAGAAAGTGGTGGTTTTGAACCAAGGGGCAGTGGTGGCCGTGAGCATGATGCTTCGGGAGTTAATTCAGAAGGCGAGCGAATATCAGATCGATCAGCAGGTAATGAAAGCACTAAATCTGATGTCACAATTGATGATGTTGCAGACTGCGAGATTCCATGGGAGGACATCACCTTGGGTGAACGTATTGGACTTGGATCGTATGGGGAGGTCTATCATGGAGACTGGCATGGCACTGAGGTTGCTGTGAAGAAGTTTCTAGACCAAGACTTATTGGGCGAATCACTTGATGAATTCAGAAGTGAGGTTCGGATGATGAAAAGACTCAGGCATCCTAATGTTGTTCTCTTCATGGGTGCGATAACTCGCGCTCCAAATCTTTCCATTGTTACAGAATTTCTTCCAAGAGGTAGTTTATACAGGTTATTACACCGGCCTAACAATCAATTAGATGAGCGGAGGCGCTTGAGGATGGCTCTTGATGCTGCTCGCGGAATGAATTACTTGCATAACTGCACTCCAGTAATTGTACACCGTGATTTGAAGTCTCCAAATCTTCTTGTCGATAGGAACTGGGTAGTGAAGGTCTGTGATTTTGGATTATCACGAATGAAGAACAGCACATTTCTCTCCTCGAGGTCGACTGCAGGGACGGCTGAATGGATGGCTCCGGAAGTGCTAAGAAATGAACCTTCGGATGAAAAATGCGATGTTTATAGCTACGGGGTCATTTTATGGGAGCTCTCTACGATGCAACAGCCATGGGGAGGAATGAACCCAATGCAAGTTGTTGGTGCAGTTGGATTTCAACATCGCCGTCTTGATATTCCAGATAATATAGATCCTGCAATTGCAGATCTCATTCGAAAATGCTGGCAAACAGATCCAAAATTGAGACCGTCGTTTGCTGAGATCATGGCAATTCTGAAACCATTGCAGAAGCCGATAAGTAGAAGTGGACAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009651 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0035556 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

1029

Amino Acids

112.74

Weight (kDa)

5.5

Isoelectric Point (pI)

49.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 160 - 362 1.6e-74 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 763 - 1014 6.3e-67 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 765 - 1012 4.2e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 2 cut(s) 340, 2752
Acc16I TGCGCA 1 cut(s) 1437
Acc36I ACCTGC 2 cut(s) 1486, 2207
AccBSI CCGCTC 1 cut(s) 2575
AccI GTMKAC 1 cut(s) 2759
AccII CGCG 3 cut(s) 321, 2487, 2610
AccIII TCCGGA 1 cut(s) 2788
AciI CCGC 7 cut(s) 496, 1937, 1940, 1943, 1947, 2575, 2610
AclWI GGATC 8 cut(s) 401, 414, 728, 1111, 1440, 2323, 2954, 2996
AcoI YGGCCR 3 cut(s) 1867, 1944, 2155
AcsI RAATTY 7 cut(s) 187, 1258, 1331, 1364, 1709, 2411, 2513
AcuI CTGAAG 3 cut(s) 501, 1341, 1482
AcyI GRCGYC 1 cut(s) 1833
AfaI GTAC 5 cut(s) 337, 1231, 1789, 1999, 2649
AfiI CCNNNNNNNGG 8 cut(s) 167, 168, 712, 1254, 1455, 1662, 1823, 2885
AflII CTTAAG 2 cut(s) 14, 1811
AflIII ACRYGT 1 cut(s) 825
AjnI CCWGG 3 cut(s) 641, 1072, 1247
Alw21I GWGCWC 2 cut(s) 463, 2862
Alw26I GTCTC 6 cut(s) 1149, 1646, 2015, 2334, 2670, 3007
AlwI GGATC 8 cut(s) 401, 414, 728, 1111, 1440, 2323, 2954, 2996
AlwNI CAGNNNCTG 3 cut(s) 1073, 1955, 2033
Ama87I CYCGRG 4 cut(s) 130, 166, 340, 2752
Aor13HI TCCGGA 1 cut(s) 2788
AoxI GGCC 8 cut(s) 79, 208, 702, 1733, 1867, 1944, 2155, 2552
ApoI RAATTY 7 cut(s) 187, 1258, 1331, 1364, 1709, 2411, 2513
Asp700I GAANNNNTTC 2 cut(s) 595, 2517
AspLEI GCGC 4 cut(s) 321, 1438, 2489, 2583
AspS9I GGNCC 6 cut(s) 79, 135, 209, 703, 803, 1002
AsuC2I CCSGG 3 cut(s) 1000, 1664, 2097
AsuHPI GGTGA 5 cut(s) 631, 991, 1783, 2281, 2309
AsuII TTCGAA 2 cut(s) 230, 2983
AvaI CYCGRG 4 cut(s) 130, 166, 340, 2752
AvaII GGWCC 3 cut(s) 135, 803, 1002
BalI TGGCCA 1 cut(s) 1869
BamHI GGATCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 2862
BauI CACGAG 1 cut(s) 1308
Bbv12I GWGCWC 2 cut(s) 463, 2862
BbvCI CCTCAGC 1 cut(s) 1761
BccI CCATC 8 cut(s) 147, 229, 536, 1057, 1096, 2105, 2584, 2776
BceAI ACGGC 4 cut(s) 1466, 2142, 2787, 2919
BciT130I CCWGG 3 cut(s) 643, 1074, 1249
BciVI GTATCC 1 cut(s) 301
BcnI CCSGG 3 cut(s) 1000, 1664, 2097
BcoDI GTCTC 6 cut(s) 1149, 1646, 2015, 2334, 2670, 3007
BfaI CTAG 9 cut(s) 213, 333, 399, 707, 1152, 1550, 1785, 2378, 3085
BfmI CTRYAG 5 cut(s) 63, 1932, 1983, 2049, 2763
BfoI RGCGCY 1 cut(s) 2584
BfrI CTTAAG 2 cut(s) 14, 1811
BfuAI ACCTGC 2 cut(s) 1486, 2207
BfuI GTATCC 1 cut(s) 301
BglII AGATCT 2 cut(s) 1472, 2974
BmcAI AGTACT 2 cut(s) 1789, 1999
Bme1390I CCNGG 6 cut(s) 643, 1000, 1074, 1249, 1664, 2097
Bme18I GGWCC 3 cut(s) 135, 803, 1002
BmeT110I CYCGRG 4 cut(s) 130, 166, 340, 2752
BmgT120I GGNCC 6 cut(s) 79, 135, 209, 703, 803, 1002
BmiI GGNNCC 6 cut(s) 80, 137, 408, 868, 1003, 2787
BmrFI CCNGG 6 cut(s) 643, 1000, 1074, 1249, 1664, 2097
BmrI ACTGGG 1 cut(s) 2701
BmuI ACTGGG 1 cut(s) 2701
BoxI GACNNNNGTC 1 cut(s) 2007
BplI GAGNNNNNCTC 4 cut(s) 566, 598, 2578, 2610
BpmI CTGGAG 4 cut(s) 459, 1461, 1884, 2622
Bpu10I CCTNAGC 1 cut(s) 1761
Bpu14I TTCGAA 2 cut(s) 230, 2983
BpuEI CTTGAG 1 cut(s) 2605
BpuMI CCSGG 3 cut(s) 1000, 1664, 2097
Bsa29I ATCGAT 1 cut(s) 2209
BsaAI YACGTR 1 cut(s) 1577
BsaHI GRCGYC 1 cut(s) 1833
BsaI GGTCTC 2 cut(s) 1646, 3007
BsaJI CCNNGG 7 cut(s) 642, 1072, 1533, 2141, 2276, 2292, 2879
BsaWI WCCGGW 3 cut(s) 1793, 1801, 2788
BsaXI ACNNNNNCTCC 2 cut(s) 2473, 2503
Bsc4I CCNNNNNNNGG 8 cut(s) 167, 168, 712, 1254, 1455, 1662, 1823, 2885
Bse118I RCCGGY 2 cut(s) 972, 2550
Bse3DI GCAATG 2 cut(s) 1023, 3050
BseAI TCCGGA 1 cut(s) 2788
BseBI CCWGG 3 cut(s) 643, 1074, 1249
BseCI ATCGAT 1 cut(s) 2209
BseDI CCNNGG 7 cut(s) 642, 1072, 1533, 2141, 2276, 2292, 2879
BseLI CCNNNNNNNGG 8 cut(s) 167, 168, 712, 1254, 1455, 1662, 1823, 2885
BseMI GCAATG 2 cut(s) 1023, 3050
BseMII CTCAG 5 cut(s) 141, 1775, 2346, 2458, 3018
BseRI GAGGAG 2 cut(s) 1643, 2740
BseX3I CGGCCG 1 cut(s) 1944
BsgI GTGCAG 3 cut(s) 1421, 2617, 2934
Bsh1236I CGCG 3 cut(s) 321, 2487, 2610
Bsh1285I CGRYCG 1 cut(s) 1947
BshFI GGCC 8 cut(s) 81, 210, 704, 1735, 1869, 1946, 2157, 2554
BshVI ATCGAT 1 cut(s) 2209
BsiEI CGRYCG 1 cut(s) 1947
BsiHKAI GWGCWC 2 cut(s) 463, 2862
BsiHKCI CYCGRG 4 cut(s) 130, 166, 340, 2752
BsiSI CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2551, 2789
BslFI GGGAC 5 cut(s) 121, 201, 1871, 1907, 2782
BslI CCNNNNNNNGG 8 cut(s) 167, 168, 712, 1254, 1455, 1662, 1823, 2885
BsmAI GTCTC 6 cut(s) 1149, 1646, 2015, 2334, 2670, 3007
BsmFI GGGAC 5 cut(s) 121, 201, 1871, 1907, 2782
BsmI GAATGC 1 cut(s) 597
BsnI GGCC 8 cut(s) 81, 210, 704, 1735, 1869, 1946, 2157, 2554
Bso31I GGTCTC 2 cut(s) 1646, 3007
BsoBI CYCGRG 4 cut(s) 130, 166, 340, 2752
Bsp119I TTCGAA 2 cut(s) 230, 2983
Bsp1286I GDGCHC 2 cut(s) 463, 2862
Bsp13I TCCGGA 1 cut(s) 2788
Bsp1407I TGTACA 1 cut(s) 2647
Bsp19I CCATGG 2 cut(s) 2276, 2879
BspACI CCGC 7 cut(s) 496, 1937, 1940, 1943, 1947, 2575, 2610
BspANI GGCC 8 cut(s) 81, 210, 704, 1735, 1869, 1946, 2157, 2554
BspCNI CTCAG 5 cut(s) 142, 1774, 2347, 2457, 3019
BspDI ATCGAT 1 cut(s) 2209
BspEI TCCGGA 1 cut(s) 2788
BspFNI CGCG 3 cut(s) 321, 2487, 2610
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 6 cut(s) 80, 137, 408, 868, 1003, 2787
BspMAI CTGCAG 5 cut(s) 67, 1936, 1987, 2053, 2767
BspMI ACCTGC 2 cut(s) 1486, 2207
BspPI GGATC 8 cut(s) 401, 414, 728, 1111, 1440, 2323, 2954, 2996
BspT104I TTCGAA 2 cut(s) 230, 2983
BspTI CTTAAG 2 cut(s) 14, 1811
BspTNI GGTCTC 2 cut(s) 1646, 3007
BsrBI CCGCTC 1 cut(s) 2575
BsrDI GCAATG 2 cut(s) 1023, 3050
BsrFI RCCGGY 2 cut(s) 972, 2550
BsrGI TGTACA 1 cut(s) 2647
BssAI RCCGGY 2 cut(s) 972, 2550
BssECI CCNNGG 7 cut(s) 642, 1072, 1533, 2141, 2276, 2292, 2879
BssNI GRCGYC 1 cut(s) 1833
BssSI CACGAG 1 cut(s) 1308
BssT1I CCWWGG 5 cut(s) 1533, 2141, 2276, 2292, 2879
Bst2BI CACGAG 1 cut(s) 1308
Bst2UI CCWGG 3 cut(s) 643, 1074, 1249
Bst4CI ACNGT 6 cut(s) 857, 1648, 1887, 2071, 2654, 3018
Bst6I CTCTTC 3 cut(s) 357, 1281, 2471
BstACI GRCGYC 1 cut(s) 1833
BstAFI CTTAAG 2 cut(s) 14, 1811
BstAPI GCANNNNNTGC 2 cut(s) 969, 2033
BstAUI TGTACA 1 cut(s) 2647
BstBAI YACGTR 1 cut(s) 1577
BstBI TTCGAA 2 cut(s) 230, 2983
BstC8I GCNNGC 4 cut(s) 1737, 2195, 2608, 2993
BstDEI CTNAG 8 cut(s) 150, 518, 848, 1761, 2355, 2444, 2798, 3027
BstDSI CCRYGG 2 cut(s) 2276, 2879
BstENI CCTNNNNNAGG 1 cut(s) 1821
BstFNI CGCG 3 cut(s) 321, 2487, 2610
BstH2I RGCGCY 1 cut(s) 2584
BstHHI GCGC 4 cut(s) 321, 1438, 2489, 2583
BstMAI GTCTC 6 cut(s) 1149, 1646, 2015, 2334, 2670, 3007
BstMCI CGRYCG 1 cut(s) 1947
BstNI CCWGG 3 cut(s) 643, 1074, 1249
BstNSI RCATGY 1 cut(s) 829
BstPAI GACNNNNGTC 1 cut(s) 2007
BstSCI CCNGG 6 cut(s) 641, 998, 1072, 1247, 1662, 2095
BstSFI CTRYAG 5 cut(s) 63, 1932, 1983, 2049, 2763
BstUI CGCG 3 cut(s) 321, 2487, 2610
BstX2I RGATCY 7 cut(s) 406, 733, 1445, 1472, 2959, 2974, 3001
BstXI CCANNNNNNTGG 2 cut(s) 800, 1012
BstYI RGATCY 7 cut(s) 406, 733, 1445, 1472, 2959, 2974, 3001
BstZI CGGCCG 1 cut(s) 1944
Bsu15I ATCGAT 1 cut(s) 2209
BsuI GTATCC 1 cut(s) 301
BsuRI GGCC 8 cut(s) 81, 210, 704, 1735, 1869, 1946, 2157, 2554
BsuTUI ATCGAT 1 cut(s) 2209
BtgI CCRYGG 2 cut(s) 2276, 2879
BtgZI GCGATG 2 cut(s) 2841, 2915
BtsI GCAGTG 2 cut(s) 2037, 2155
BtsIMutI CAGTG 4 cut(s) 1012, 2037, 2155, 2352
BveI ACCTGC 2 cut(s) 1486, 2207
Cac8I GCNNGC 4 cut(s) 1737, 2195, 2608, 2993
CaiI CAGNNNCTG 3 cut(s) 1073, 1955, 2033
CciI TCATGA 1 cut(s) 1564
CciNI GCGGCCGC 1 cut(s) 1944
CfoI GCGC 4 cut(s) 321, 1438, 2489, 2583
Cfr10I RCCGGY 2 cut(s) 972, 2550
Cfr13I GGNCC 6 cut(s) 79, 135, 209, 703, 803, 1002
ClaI ATCGAT 1 cut(s) 2209
CseI GACGC 1 cut(s) 1822
Csp6I GTAC 5 cut(s) 336, 1230, 1788, 1998, 2648
CviQI GTAC 5 cut(s) 336, 1230, 1788, 1998, 2648
DdeI CTNAG 8 cut(s) 150, 518, 848, 1761, 2355, 2444, 2798, 3027
DraI TTTAAA 1 cut(s) 1714
EaeI YGGCCR 3 cut(s) 1867, 1944, 2155
EagI CGGCCG 1 cut(s) 1944
Eam1104I CTCTTC 3 cut(s) 357, 1281, 2471
EarI CTCTTC 3 cut(s) 357, 1281, 2471
Ecl136II GAGCTC 1 cut(s) 2860
EclXI CGGCCG 1 cut(s) 1944
Eco130I CCWWGG 5 cut(s) 1533, 2141, 2276, 2292, 2879
Eco24I GRGCYC 1 cut(s) 2862
Eco31I GGTCTC 2 cut(s) 1646, 3007
Eco47I GGWCC 3 cut(s) 135, 803, 1002
Eco52I CGGCCG 1 cut(s) 1944
Eco53kI GAGCTC 1 cut(s) 2860
Eco57I CTGAAG 3 cut(s) 501, 1341, 1482
Eco88I CYCGRG 4 cut(s) 130, 166, 340, 2752
EcoICRI GAGCTC 1 cut(s) 2860
EcoNI CCTNNNNNAGG 1 cut(s) 1821
EcoO109I RGGNCCY 1 cut(s) 135
EcoRI GAATTC 3 cut(s) 187, 1331, 2411
EcoRII CCWGG 3 cut(s) 641, 1072, 1247
EcoT14I CCWWGG 5 cut(s) 1533, 2141, 2276, 2292, 2879
EcoT38I GRGCYC 1 cut(s) 2862
ErhI CCWWGG 5 cut(s) 1533, 2141, 2276, 2292, 2879
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 5 cut(s) 121, 201, 1871, 1907, 2782
FauNDI CATATG 1 cut(s) 795
FblI GTMKAC 1 cut(s) 2759
FriOI GRGCYC 1 cut(s) 2862
FspBI CTAG 9 cut(s) 213, 333, 399, 707, 1152, 1550, 1785, 2378, 3085
FspI TGCGCA 1 cut(s) 1437
GlaI GCGC 4 cut(s) 320, 1437, 2488, 2582
GsuI CTGGAG 4 cut(s) 459, 1461, 1884, 2622
HaeII RGCGCY 1 cut(s) 2584
HaeIII GGCC 8 cut(s) 81, 210, 704, 1735, 1869, 1946, 2157, 2554
HapII CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2551, 2789
HgaI GACGC 1 cut(s) 1822
HhaI GCGC 4 cut(s) 321, 1438, 2489, 2583
Hin1I GRCGYC 1 cut(s) 1833
Hin6I GCGC 4 cut(s) 319, 1436, 2487, 2581
HinP1I GCGC 4 cut(s) 319, 1436, 2487, 2581
HincII GTYRAC 6 cut(s) 238, 579, 1644, 1799, 1837, 2760
HindII GTYRAC 6 cut(s) 238, 579, 1644, 1799, 1837, 2760
HindIII AAGCTT 1 cut(s) 1559
HpaII CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2551, 2789
HphI GGTGA 5 cut(s) 631, 991, 1783, 2281, 2309
Hpy99I CGWCG 1 cut(s) 3022
HpyCH4III ACNGT 6 cut(s) 857, 1648, 1887, 2071, 2654, 3018
HpyCH4IV ACGT 4 cut(s) 1383, 1576, 1860, 2302
HpyF3I CTNAG 8 cut(s) 150, 518, 848, 1761, 2355, 2444, 2798, 3027
HpySE526I ACGT 4 cut(s) 1383, 1576, 1860, 2302
Hsp92I GRCGYC 1 cut(s) 1833
HspAI GCGC 4 cut(s) 319, 1436, 2487, 2581
Kpn2I TCCGGA 1 cut(s) 2788
LmnI GCTCC 6 cut(s) 256, 466, 1600, 2494, 2791, 2857
MaeI CTAG 9 cut(s) 213, 333, 399, 707, 1152, 1550, 1785, 2378, 3085
MaeII ACGT 4 cut(s) 1383, 1576, 1860, 2302
MaeIII GTNAC 5 cut(s) 637, 851, 1861, 2242, 2506
MbiI CCGCTC 1 cut(s) 2575
MfeI CAATTG 3 cut(s) 377, 2247, 2967
MflI RGATCY 7 cut(s) 406, 733, 1445, 1472, 2959, 2974, 3001
MhlI GDGCHC 2 cut(s) 463, 2862
MlsI TGGCCA 1 cut(s) 1869
MluNI TGGCCA 1 cut(s) 1869
MlyI GAGTC 2 cut(s) 584, 2436
MmeI TCCRAC 2 cut(s) 60, 2899
Mox20I TGGCCA 1 cut(s) 1869
MroI TCCGGA 1 cut(s) 2788
MroXI GAANNNNTTC 2 cut(s) 595, 2517
MscI TGGCCA 1 cut(s) 1869
MslI CAYNNNNRTG 2 cut(s) 798, 2454
Msp20I TGGCCA 1 cut(s) 1869
MspA1I CMGCKG 7 cut(s) 275, 479, 1100, 1937, 1955, 2030, 2054
MspCI CTTAAG 2 cut(s) 14, 1811
MspI CCGG 8 cut(s) 973, 999, 1663, 1794, 1802, 2096, 2551, 2789
MspR9I CCNGG 6 cut(s) 643, 1000, 1074, 1249, 1664, 2097
MunI CAATTG 3 cut(s) 377, 2247, 2967
Mva1269I GAATGC 1 cut(s) 597
MvaI CCWGG 3 cut(s) 643, 1074, 1249
MvnI CGCG 3 cut(s) 321, 2487, 2610
NciI CCSGG 3 cut(s) 1000, 1664, 2097
NcoI CCATGG 2 cut(s) 2276, 2879
NdeI CATATG 1 cut(s) 795
NlaIV GGNNCC 6 cut(s) 80, 137, 408, 868, 1003, 2787
NmuCI GTSAC 2 cut(s) 637, 2242
NotI GCGGCCGC 1 cut(s) 1944
NsbI TGCGCA 1 cut(s) 1437
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 2 cut(s) 230, 2983
PaeR7I CTCGAG 3 cut(s) 130, 340, 2752
PagI TCATGA 1 cut(s) 1564
PciI ACATGT 1 cut(s) 825
PctI GAATGC 1 cut(s) 597
PdmI GAANNNNTTC 2 cut(s) 595, 2517
PflFI GACNNNGTC 2 cut(s) 533, 574
PleI GAGTC 2 cut(s) 583, 2436
PpsI GAGTC 2 cut(s) 583, 2436
Ppu21I YACGTR 1 cut(s) 1577
PpuMI RGGWCCY 1 cut(s) 135
PscI ACATGT 1 cut(s) 825
PshAI GACNNNNGTC 1 cut(s) 2007
Psp124BI GAGCTC 1 cut(s) 2862
Psp5II RGGWCCY 1 cut(s) 135
Psp6I CCWGG 3 cut(s) 641, 1072, 1247
PspGI CCWGG 3 cut(s) 641, 1072, 1247
PspN4I GGNNCC 6 cut(s) 80, 137, 408, 868, 1003, 2787
PspPI GGNCC 6 cut(s) 79, 135, 209, 703, 803, 1002
PspPPI RGGWCCY 1 cut(s) 135
PspXI VCTCGAGB 3 cut(s) 130, 340, 2752
PstI CTGCAG 5 cut(s) 67, 1936, 1987, 2053, 2767
PstNI CAGNNNCTG 3 cut(s) 1073, 1955, 2033
PsuI RGATCY 7 cut(s) 406, 733, 1445, 1472, 2959, 2974, 3001
PsyI GACNNNGTC 2 cut(s) 533, 574
PvuII CAGCTG 6 cut(s) 275, 479, 1100, 1955, 2030, 2054
RsaI GTAC 5 cut(s) 337, 1231, 1789, 1999, 2649
RsaNI GTAC 5 cut(s) 336, 1230, 1788, 1998, 2648
RseI CAYNNNNRTG 2 cut(s) 798, 2454
SacI GAGCTC 1 cut(s) 2862
SalI GTCGAC 1 cut(s) 2758
Sau96I GGNCC 6 cut(s) 79, 135, 209, 703, 803, 1002
ScaI AGTACT 2 cut(s) 1789, 1999
SchI GAGTC 2 cut(s) 584, 2436
ScrFI CCNGG 6 cut(s) 643, 1000, 1074, 1249, 1664, 2097
SduI GDGCHC 2 cut(s) 463, 2862
SfcI CTRYAG 5 cut(s) 63, 1932, 1983, 2049, 2763
Sfr274I CTCGAG 3 cut(s) 130, 340, 2752
SfuI TTCGAA 2 cut(s) 230, 2983
SinI GGWCC 3 cut(s) 135, 803, 1002
SlaI CTCGAG 3 cut(s) 130, 340, 2752
SmiMI CAYNNNNRTG 2 cut(s) 798, 2454
SmlI CTYRAG 6 cut(s) 14, 130, 340, 1811, 2584, 2752
SmoI CTYRAG 6 cut(s) 14, 130, 340, 1811, 2584, 2752
SsiI CCGC 7 cut(s) 496, 1937, 1940, 1943, 1947, 2575, 2610
SspI AATATT 1 cut(s) 567
SspMI CTAG 9 cut(s) 213, 333, 399, 707, 1152, 1550, 1785, 2378, 3085
SstI GAGCTC 1 cut(s) 2862
StyD4I CCNGG 6 cut(s) 641, 998, 1072, 1247, 1662, 2095
StyI CCWWGG 5 cut(s) 1533, 2141, 2276, 2292, 2879
TaaI ACNGT 6 cut(s) 857, 1648, 1887, 2071, 2654, 3018
TaiI ACGT 4 cut(s) 1386, 1579, 1863, 2305
TatI WGTACW 3 cut(s) 1787, 1997, 2647
TauI GCSGC 4 cut(s) 1940, 1943, 1946, 1949
TscAI CASTG 4 cut(s) 1012, 2044, 2155, 2359
TseFI GTSAC 2 cut(s) 637, 2242
Tsp45I GTSAC 2 cut(s) 637, 2242
TspGWI ACGGA 1 cut(s) 587
TspRI CASTG 4 cut(s) 1012, 2044, 2155, 2359
Tth111I GACNNNGTC 2 cut(s) 533, 574
Vha464I CTTAAG 2 cut(s) 14, 1811
VpaK11BI GGWCC 3 cut(s) 135, 803, 1002
XagI CCTNNNNNAGG 1 cut(s) 1821
XapI RAATTY 7 cut(s) 187, 1258, 1331, 1364, 1709, 2411, 2513
XbaI TCTAGA 2 cut(s) 1151, 2377
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XhoI CTCGAG 3 cut(s) 130, 340, 2752
XmiI GTMKAC 1 cut(s) 2759
XmnI GAANNNNTTC 2 cut(s) 595, 2517
XspI CTAG 9 cut(s) 213, 333, 399, 707, 1152, 1550, 1785, 2378, 3085
ZrmI AGTACT 2 cut(s) 1789, 1999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.