Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003167

Physical Location & Seq

Genomic Coordinates Forward (+)

5045252 .. 5055639

Gene Structure 10388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1054600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1338 bp

ATGGCCGGGAACTGCCTGCAAGCCGCGGAGCTAACCACGAGTGGCCGCCCAGTCCTTCTCCCAACTGAGATCGAATGTTCCCTCCTTTCCGCCGTAGACCTCGAATGCGAAGACCTCCCCAACTTCCCGCACCTTAAATTGGGTCTCCTCATTCTCACCACCCACCGCCTCTTATGGCTTCCCGACTCCACCACCACAACAGCCACCGCCATCCCTCTCGCTGCCATCGCCCACATCTTTGCCGCCAAAAAGTCAATCAAGTCCATGTTCGCCTGGCCCCGGGTCAGATTCCAGCTCTCGGTTTCGCCCGAAGGCCGGGTTCCGGATTCGGGTTCGGGTTCGAGGTCGGTGGTTGTGACGGTGGTGGTGAGGGGGAAGGGCGATTTGGACGCGTTTTTGAGCAAATTTTGGGAGAGCTGGCGGGGTAGAGCCTGGGAGATTGCGGACGAGCAGGGCAGCTCCGATGCGGCTTCAGGTTCGGGTTCGGCGTCCAGTTCCGGGTTGTATACGAGGGAAGGGACGGTGAGGATGGTTGGAGTTTCGGGGATATTGAGGAAAGAGCAGGAAATGTGGGAGAGTACTGATAAGAGCTTGCAGGATGCTTTCCAGGACTTGAATGCTCTCATGAGTAAGGCTAAAGAGATGGTGATGCTAGCAGAAAAAATGAGGCAGAAGCTTTTATCTGGCTCGACTTCGCAGCCAAATGATGAGGAATTGGGTTCCAAACAAGAGATGCAGGATTGGATGCTGAGTGTTGGCATTATATCGCCCGTTACTAAGGAGTCTGCTGGTTCTCAGTATCACCAACAATTGTCTCGTCAGTTGGCAGATTTTGTCAGACTTCCACTTGAGAGAGCTGGAGGAATGATGAATCTTATAGACATCTACTGTCTCTTCAATCGTGCTCGAGGCACAGAACTGATCTCACCAGAGGATTTGCTGCAAGCGTGTTCGCTCTGGGAGAAATTTGATGTCCCAGTAATGCTTCGAAGGTTTGATAGTGGAGTCATGGTCATCCAGAATAAGTCCCACAGTGACGAAGAGGTTTTCGCTAGAATCAAGACCCTTGTAACAAAGCCTGATGCTCTTAGAACTGGGATAAGTGCTAGTGATGCTGCAATTACATTAAGTATTGCACCAGGCATGGCCAAGGAGCATCTTCTTACTGCTGAAAGCAAAGGTTTGCTGTGCAGAGATATAAGCCCTGATGGCTTTCGCTTTTATATCAACCTGTTTCCCGAAATCGATCCAAATGACTTGTACCTTGAAAGATTATGGAATCTATCACACATGGTTGAGGGCGGCGTCTGCTTCTGGATGAGAGGAGTTAAGACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000814 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007033 GO:0007034 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010008 GO:0010154 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0019538 GO:0019941 GO:0022414 GO:0030163 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032509 GO:0032511 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0036452 GO:0042886 GO:0043130 GO:0043162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043328 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045324 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0061458 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071944 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0090351 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EAP30 Vps36_ESCRT-II

Protein Analysis

446

Amino Acids

49.14

Weight (kDa)

5.56

Isoelectric Point (pI)

48.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps36_ESCRT-II	PF11605	10 - 99	4.3e-10	Vacuolar protein sorting protein 36 Vps36
EAP30	PF04157	178 - 399	5.9e-58	EAP30/Vps36 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04920
fragaria_vesca	FvH4_4g11870 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040
malus_domestica	MD02G1263600.v1.1 MD07G1054600.v1.1
prunus_persica	Prupe.2G061300_v2.0.a1 Prupe.2G061300_v2.0.a1 Prupe.2G061300_v2.0.a1
pyrus_communis	pycom02g22580 pycom07g04240
rosa_chinensis	RchiOBHm_Chr1g0331361
rosa_laevigata	RLG00000029741
rosa_multiflora	Rmu_sc0005956.1_g000008
rosa_roxburghii	Rroxscaffold_4G00319220
rosa_rugosa	Rorug01G0092800
rosa_samantha	Rh1AG117800 Rh1BG088500 Rh1CG112700 Rh1DG121600
rosa_wichuraiana	Rw1G009420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	96, 506
AccII	CGCG	2 cut(s)	26, 392
AccIII	TCCGGA	1 cut(s)	322
AclWI	GGATC	1 cut(s)	1241
AcoI	YGGCCR	3 cut(s)	3, 43, 1146
AcsI	RAATTY	2 cut(s)	404, 965
AcuI	CTGAAG	1 cut(s)	456
AcyI	GRCGYC	2 cut(s)	488, 1305
AfaI	GTAC	2 cut(s)	580, 1262
AfiI	CCNNNNNNNGG	7 cut(s)	139, 279, 298, 315, 322, 329, 498
AflIII	ACRYGT	1 cut(s)	390
AgsI	TTSAA	3 cut(s)	616, 898, 1268
AjnI	CCWGG	4 cut(s)	272, 431, 606, 1138
AjuI	GAANNNNNNNTTGG	2 cut(s)	368, 400
AluBI	AGCT	7 cut(s)	31, 295, 417, 459, 591, 676, 857
AluI	AGCT	7 cut(s)	31, 295, 417, 459, 591, 676, 857
Alw21I	GWGCWC	1 cut(s)	907
Alw26I	GTCTC	3 cut(s)	149, 819, 896
AlwI	GGATC	1 cut(s)	1241
Ama87I	CYCGRG	2 cut(s)	279, 906
Aor13HI	TCCGGA	1 cut(s)	322
AoxI	GGCC	5 cut(s)	3, 43, 275, 313, 1146
ApeKI	GCWGC	5 cut(s)	221, 456, 697, 940, 1115
ApoI	RAATTY	2 cut(s)	404, 965
AspS9I	GGNCC	1 cut(s)	276
AsuC2I	CCSGG	5 cut(s)	7, 280, 281, 317, 499
AsuHPI	GGTGA	6 cut(s)	148, 379, 535, 658, 794, 918
AsuII	TTCGAA	1 cut(s)	988
AsuNHI	GCTAGC	1 cut(s)	652
AvaI	CYCGRG	2 cut(s)	279, 906
BalI	TGGCCA	1 cut(s)	1148
BauI	CACGAG	1 cut(s)	37
BbsI	GAAGAC	1 cut(s)	117
Bbv12I	GWGCWC	1 cut(s)	907
BbvI	GCAGC	5 cut(s)	208, 468, 709, 927, 1102
BccI	CCATC	5 cut(s)	218, 233, 523, 637, 1202
BceAI	ACGGC	1 cut(s)	77
BciT130I	CCWGG	4 cut(s)	274, 433, 608, 1140
BcnI	CCSGG	5 cut(s)	7, 280, 281, 317, 499
BcoDI	GTCTC	3 cut(s)	149, 819, 896
BfaI	CTAG	3 cut(s)	653, 1053, 1107
BglI	GCCNNNNNGGC	1 cut(s)	1209
BmcAI	AGTACT	1 cut(s)	580
Bme1390I	CCNGG	9 cut(s)	7, 274, 280, 281, 317, 433, 499, 608, 1140
BmeT110I	CYCGRG	2 cut(s)	279, 906
BmgT120I	GGNCC	1 cut(s)	276
BmiI	GGNNCC	3 cut(s)	278, 321, 721
BmrFI	CCNGG	9 cut(s)	7, 274, 280, 281, 317, 433, 499, 608, 1140
BmrI	ACTGGG	3 cut(s)	44, 971, 1104
BmsI	GCATC	8 cut(s)	454, 589, 639, 723, 735, 1072, 1102, 1165
BmtI	GCTAGC	1 cut(s)	656
BmuI	ACTGGG	3 cut(s)	44, 971, 1104
BpiI	GAAGAC	1 cut(s)	117
BpmI	CTGGAG	1 cut(s)	879
Bpu14I	TTCGAA	1 cut(s)	988
BpuEI	CTTGAG	1 cut(s)	869
BpuMI	CCSGG	5 cut(s)	7, 280, 281, 317, 499
Bsa29I	ATCGAT	1 cut(s)	1245
BsaHI	GRCGYC	2 cut(s)	488, 1305
BsaI	GGTCTC	1 cut(s)	149
BsaJI	CCNNGG	5 cut(s)	24, 278, 279, 432, 1149
BsaWI	WCCGGW	1 cut(s)	322
BsaXI	ACNNNNNCTCC	2 cut(s)	996, 1026
Bsc4I	CCNNNNNNNGG	7 cut(s)	139, 279, 298, 315, 322, 329, 498
Bse1I	ACTGG	4 cut(s)	50, 492, 977, 1099
BseAI	TCCGGA	1 cut(s)	322
BseBI	CCWGG	4 cut(s)	274, 433, 608, 1140
BseCI	ATCGAT	1 cut(s)	1245
BseDI	CCNNGG	5 cut(s)	24, 278, 279, 432, 1149
BseGI	GGATG	6 cut(s)	210, 534, 604, 750, 1014, 1323
BseLI	CCNNNNNNNGG	7 cut(s)	139, 279, 298, 315, 322, 329, 498
BseMII	CTCAG	3 cut(s)	57, 740, 809
BseNI	ACTGG	4 cut(s)	50, 492, 977, 1099
BseRI	GAGGAG	2 cut(s)	137, 1338
BseXI	GCAGC	5 cut(s)	208, 468, 709, 927, 1102
BsgI	GTGCAG	1 cut(s)	1210
Bsh1236I	CGCG	2 cut(s)	26, 392
BshFI	GGCC	5 cut(s)	5, 45, 277, 315, 1148
BshVI	ATCGAT	1 cut(s)	1245
BsiHKAI	GWGCWC	1 cut(s)	907
BsiHKCI	CYCGRG	2 cut(s)	279, 906
BsiSI	CCGG	5 cut(s)	6, 280, 316, 323, 498
BslFI	GGGAC	3 cut(s)	532, 959, 1012
BslI	CCNNNNNNNGG	7 cut(s)	139, 279, 298, 315, 322, 329, 498
BsmAI	GTCTC	3 cut(s)	149, 819, 896
BsmFI	GGGAC	3 cut(s)	532, 959, 1012
BsmI	GAATGC	2 cut(s)	110, 622
BsnI	GGCC	5 cut(s)	5, 45, 277, 315, 1148
Bso31I	GGTCTC	1 cut(s)	149
BsoBI	CYCGRG	2 cut(s)	279, 906
Bsp119I	TTCGAA	1 cut(s)	988
Bsp1286I	GDGCHC	1 cut(s)	907
Bsp13I	TCCGGA	1 cut(s)	322
Bsp143I	GATC	3 cut(s)	69, 921, 1246
BspANI	GGCC	5 cut(s)	5, 45, 277, 315, 1148
BspCNI	CTCAG	3 cut(s)	58, 741, 808
BspDI	ATCGAT	1 cut(s)	1245
BspEI	TCCGGA	1 cut(s)	322
BspFNI	CGCG	2 cut(s)	26, 392
BspHI	TCATGA	1 cut(s)	624
BspLI	GGNNCC	3 cut(s)	278, 321, 721
BspOI	GCTAGC	1 cut(s)	656
BspPI	GGATC	1 cut(s)	1241
BspT104I	TTCGAA	1 cut(s)	988
BspTNI	GGTCTC	1 cut(s)	149
BsrI	ACTGG	4 cut(s)	50, 492, 977, 1099
BssECI	CCNNGG	5 cut(s)	24, 278, 279, 432, 1149
BssMI	GATC	3 cut(s)	69, 921, 1246
BssNAI	GTATAC	1 cut(s)	507
BssNI	GRCGYC	2 cut(s)	488, 1305
BssSI	CACGAG	1 cut(s)	37
BssT1I	CCWWGG	1 cut(s)	1149
Bst1107I	GTATAC	1 cut(s)	507
Bst2BI	CACGAG	1 cut(s)	37
Bst2UI	CCWGG	4 cut(s)	274, 433, 608, 1140
Bst4CI	ACNGT	4 cut(s)	361, 523, 890, 1034
Bst6I	CTCTTC	2 cut(s)	899, 1035
BstACI	GRCGYC	2 cut(s)	488, 1305
BstBI	TTCGAA	1 cut(s)	988
BstC8I	GCNNGC	6 cut(s)	17, 21, 419, 593, 654, 945
BstDEI	CTNAG	5 cut(s)	66, 749, 777, 795, 1088
BstDSI	CCRYGG	1 cut(s)	24
BstF5I	GGATG	6 cut(s)	210, 534, 604, 750, 1014, 1323
BstFNI	CGCG	2 cut(s)	26, 392
BstKTI	GATC	3 cut(s)	72, 924, 1249
BstMAI	GTCTC	3 cut(s)	149, 819, 896
BstMBI	GATC	3 cut(s)	69, 921, 1246
BstMWI	GCNNNNNNNGC	4 cut(s)	227, 1112, 1209, 1308
BstNI	CCWGG	4 cut(s)	274, 433, 608, 1140
BstSCI	CCNGG	9 cut(s)	5, 272, 278, 279, 315, 431, 497, 606, 1138
BstUI	CGCG	2 cut(s)	26, 392
BstV1I	GCAGC	5 cut(s)	208, 468, 709, 927, 1102
BstV2I	GAAGAC	1 cut(s)	117
BstZ17I	GTATAC	1 cut(s)	507
Bsu15I	ATCGAT	1 cut(s)	1245
BsuRI	GGCC	5 cut(s)	5, 45, 277, 315, 1148
BsuTUI	ATCGAT	1 cut(s)	1245
BtgI	CCRYGG	1 cut(s)	24
BtgZI	GCGATG	1 cut(s)	211
BtsCI	GGATG	6 cut(s)	210, 534, 604, 750, 1014, 1323
BtsIMutI	CAGTG	1 cut(s)	1039
Cac8I	GCNNGC	6 cut(s)	17, 21, 419, 593, 654, 945
CciI	TCATGA	1 cut(s)	624
Cfr13I	GGNCC	1 cut(s)	276
Cfr42I	CCGCGG	1 cut(s)	27
Cfr9I	CCCGGG	1 cut(s)	279
ClaI	ATCGAT	1 cut(s)	1245
CseI	GACGC	3 cut(s)	398, 477, 1294
Csp6I	GTAC	2 cut(s)	579, 1261
CviAII	CATG	5 cut(s)	265, 625, 1009, 1144, 1291
CviQI	GTAC	2 cut(s)	579, 1261
DdeI	CTNAG	5 cut(s)	66, 749, 777, 795, 1088
DpnI	GATC	3 cut(s)	71, 923, 1248
DpnII	GATC	3 cut(s)	69, 921, 1246
EaeI	YGGCCR	3 cut(s)	3, 43, 1146
Eam1104I	CTCTTC	2 cut(s)	899, 1035
EarI	CTCTTC	2 cut(s)	899, 1035
EciI	GGCGGA	1 cut(s)	79
Eco130I	CCWWGG	1 cut(s)	1149
Eco31I	GGTCTC	1 cut(s)	149
Eco57I	CTGAAG	1 cut(s)	456
Eco88I	CYCGRG	2 cut(s)	279, 906
EcoRII	CCWGG	4 cut(s)	272, 431, 606, 1138
EcoT14I	CCWWGG	1 cut(s)	1149
ErhI	CCWWGG	1 cut(s)	1149
FaeI	CATG	5 cut(s)	268, 628, 1012, 1147, 1294
FaqI	GGGAC	3 cut(s)	532, 959, 1012
FatI	CATG	5 cut(s)	264, 624, 1008, 1143, 1290
FauI	CCCGC	2 cut(s)	135, 414
FblI	GTMKAC	2 cut(s)	96, 506
FokI	GGATG	6 cut(s)	197, 541, 611, 757, 1001, 1330
FspBI	CTAG	3 cut(s)	653, 1053, 1107
GsuI	CTGGAG	1 cut(s)	879
HaeIII	GGCC	5 cut(s)	5, 45, 277, 315, 1148
HapII	CCGG	5 cut(s)	6, 280, 316, 323, 498
HgaI	GACGC	3 cut(s)	398, 477, 1294
Hin1I	GRCGYC	2 cut(s)	488, 1305
Hin1II	CATG	5 cut(s)	268, 628, 1012, 1147, 1294
HindIII	AAGCTT	1 cut(s)	674
HinfI	GANTC	8 cut(s)	185, 288, 326, 782, 871, 1005, 1056, 1279
HpaII	CCGG	5 cut(s)	6, 280, 316, 323, 498
HphI	GGTGA	6 cut(s)	148, 379, 535, 658, 794, 918
Hpy166II	GTNNAC	2 cut(s)	97, 507
Hpy188I	TCNGA	3 cut(s)	287, 463, 839
Hpy188III	TCNNGA	7 cut(s)	182, 323, 625, 1018, 1060, 1238, 1315
Hpy8I	GTNNAC	2 cut(s)	97, 507
HpyAV	CCTTC	5 cut(s)	65, 305, 370, 509, 984
HpyCH4III	ACNGT	4 cut(s)	361, 523, 890, 1034
HpyCH4IV	ACGT	1 cut(s)	1334
HpyCH4V	TGCA	7 cut(s)	19, 595, 736, 943, 1118, 1136, 1191
HpyF10VI	GCNNNNNNNGC	4 cut(s)	227, 1112, 1209, 1308
HpyF3I	CTNAG	5 cut(s)	66, 749, 777, 795, 1088
HpySE526I	ACGT	1 cut(s)	1334
Hsp92I	GRCGYC	2 cut(s)	488, 1305
Hsp92II	CATG	5 cut(s)	268, 628, 1012, 1147, 1294
Kpn2I	TCCGGA	1 cut(s)	322
KspI	CCGCGG	1 cut(s)	27
Kzo9I	GATC	3 cut(s)	69, 921, 1246
LmnI	GCTCC	3 cut(s)	28, 464, 1153
Lsp1109I	GCAGC	5 cut(s)	208, 468, 709, 927, 1102
LweI	GCATC	8 cut(s)	454, 589, 639, 723, 735, 1072, 1102, 1165
MaeI	CTAG	3 cut(s)	653, 1053, 1107
MaeII	ACGT	1 cut(s)	1334
MaeIII	GTNAC	4 cut(s)	355, 772, 1034, 1069
MalI	GATC	3 cut(s)	71, 923, 1248
MboI	GATC	3 cut(s)	69, 921, 1246
MboII	GAAGA	4 cut(s)	122, 886, 1052, 1151
MfeI	CAATTG	1 cut(s)	809
MhlI	GDGCHC	1 cut(s)	907
MlsI	TGGCCA	1 cut(s)	1148
MluCI	AATT	6 cut(s)	137, 404, 713, 809, 965, 1119
MluI	ACGCGT	1 cut(s)	390
MluNI	TGGCCA	1 cut(s)	1148
MlyI	GAGTC	3 cut(s)	179, 791, 1014
MmeI	TCCRAC	1 cut(s)	514
Mox20I	TGGCCA	1 cut(s)	1148
MroI	TCCGGA	1 cut(s)	322
MscI	TGGCCA	1 cut(s)	1148
MseI	TTAA	3 cut(s)	135, 1127, 1329
Msp20I	TGGCCA	1 cut(s)	1148
MspA1I	CMGCKG	1 cut(s)	26
MspI	CCGG	5 cut(s)	6, 280, 316, 323, 498
MspR9I	CCNGG	9 cut(s)	7, 274, 280, 281, 317, 433, 499, 608, 1140
MunI	CAATTG	1 cut(s)	809
Mva1269I	GAATGC	2 cut(s)	110, 622
MvaI	CCWGG	4 cut(s)	274, 433, 608, 1140
MvnI	CGCG	2 cut(s)	26, 392
MwoI	GCNNNNNNNGC	4 cut(s)	227, 1112, 1209, 1308
NciI	CCSGG	5 cut(s)	7, 280, 281, 317, 499
NdeII	GATC	3 cut(s)	69, 921, 1246
NheI	GCTAGC	1 cut(s)	652
NlaIII	CATG	5 cut(s)	268, 628, 1012, 1147, 1294
NlaIV	GGNNCC	3 cut(s)	278, 321, 721
NmuCI	GTSAC	2 cut(s)	355, 1034
NspV	TTCGAA	1 cut(s)	988
PaeR7I	CTCGAG	1 cut(s)	906
PagI	TCATGA	1 cut(s)	624
PcsI	WCGNNNNNNNCGW	1 cut(s)	485
PctI	GAATGC	2 cut(s)	110, 622
PfeI	GAWTC	5 cut(s)	288, 326, 871, 1056, 1279
PfoI	TCCNGGA	1 cut(s)	606
PleI	GAGTC	3 cut(s)	179, 790, 1013
PpsI	GAGTC	3 cut(s)	179, 790, 1013
Psp6I	CCWGG	4 cut(s)	272, 431, 606, 1138
PspGI	CCWGG	4 cut(s)	272, 431, 606, 1138
PspN4I	GGNNCC	3 cut(s)	278, 321, 721
PspPI	GGNCC	1 cut(s)	276
PspXI	VCTCGAGB	1 cut(s)	906
RsaI	GTAC	2 cut(s)	580, 1262
RsaNI	GTAC	2 cut(s)	579, 1261
SacII	CCGCGG	1 cut(s)	27
SaqAI	TTAA	3 cut(s)	135, 1127, 1329
Sau3AI	GATC	3 cut(s)	69, 921, 1246
Sau96I	GGNCC	1 cut(s)	276
ScaI	AGTACT	1 cut(s)	580
SchI	GAGTC	3 cut(s)	179, 791, 1014
ScrFI	CCNGG	9 cut(s)	7, 274, 280, 281, 317, 433, 499, 608, 1140
SduI	GDGCHC	1 cut(s)	907
SfaNI	GCATC	8 cut(s)	454, 589, 639, 723, 735, 1072, 1102, 1165
Sfr274I	CTCGAG	1 cut(s)	906
Sfr303I	CCGCGG	1 cut(s)	27
SfuI	TTCGAA	1 cut(s)	988
SgrBI	CCGCGG	1 cut(s)	27
SlaI	CTCGAG	1 cut(s)	906
SmaI	CCCGGG	1 cut(s)	281
SmlI	CTYRAG	2 cut(s)	848, 906
SmoI	CTYRAG	2 cut(s)	848, 906
Sse9I	AATT	6 cut(s)	137, 404, 713, 809, 965, 1119
SspMI	CTAG	3 cut(s)	653, 1053, 1107
StyD4I	CCNGG	9 cut(s)	5, 272, 278, 279, 315, 431, 497, 606, 1138
StyI	CCWWGG	1 cut(s)	1149
TaaI	ACNGT	4 cut(s)	361, 523, 890, 1034
TaiI	ACGT	1 cut(s)	1337
TaqI	TCGA	7 cut(s)	72, 102, 341, 689, 907, 988, 1245
TasI	AATT	6 cut(s)	137, 404, 713, 809, 965, 1119
TatI	WGTACW	1 cut(s)	578
TauI	GCSGC	5 cut(s)	26, 48, 245, 470, 1305
TfiI	GAWTC	5 cut(s)	288, 326, 871, 1056, 1279
Tru1I	TTAA	3 cut(s)	135, 1127, 1329
Tru9I	TTAA	3 cut(s)	135, 1127, 1329
TscAI	CASTG	1 cut(s)	1039
TseFI	GTSAC	2 cut(s)	355, 1034
TseI	GCWGC	5 cut(s)	221, 456, 697, 940, 1115
Tsp45I	GTSAC	2 cut(s)	355, 1034
TspDTI	ATGAA	1 cut(s)	884
TspMI	CCCGGG	1 cut(s)	279
TspRI	CASTG	1 cut(s)	1039
XapI	RAATTY	2 cut(s)	404, 965
XhoI	CTCGAG	1 cut(s)	906
XmaI	CCCGGG	1 cut(s)	279
XmiI	GTMKAC	2 cut(s)	96, 506
XspI	CTAG	3 cut(s)	653, 1053, 1107
ZrmI	AGTACT	1 cut(s)	580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.