Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0003167

Physical Location & Seq

Genomic Coordinates Forward (+)

46772962 .. 46781922

Gene Structure 8961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00319220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGCCGGAAATTGCTTGGAAGCTGTGGAGCTAACAAGCAGTGGACGCCCAGTCCTCCTCCCATCAGAGATTGAATGTTCGCTCCTCTCCACTGTAGACCTCGAATGCGAAGACCTCCCTAACTTCCCTAACCTCAAATCGGGTATCCTCACCCTTACCACCCACCGCCTCTTGTGGGTCTCCGACTCCTCTTCACAAGGAGCCTCTTCAGCCATCCCACTTGCCTCCATCAGCCACATCTTCTCCGCCAAAAAGTCCCTCAAGTCCATCTTTGCCTCGCCCCGGATCCGGTTCCAGCTTTCGGTGTCACCCGACGGCCGGGTTTCAAATTCAGGTTCAGGTTCTAGGTCCATCGTCGTTACAGTGGTTGTGAGGGGGAAGGGTGCTGATTTGGACGTGTTTTTGACCAAGTTTTGGGATAACTGGCGGGGAAGAGCCTGGGAGACTGGGAATGCAGATGTCACTTCGGGTTCTGAGTTGGTTTCTGGGTCGACTTCTTCTGGATTATACACCAAGGAAGGAACGGTGAGGATGGTTGGGGTGTCTGGAATACTCAGGAAGGAACAAGAGATGTGGGAGACTACTGATAAGAGCTTGCAGGATGCTTTCCAGGACTTGAATGCTCTCATGAGTAAAGCTAAAGAGATGGTGATGCTAGCAGAGAAAATGAGGCAGAAACTTTTATCCGGCTCGACTTCTCAGCCTGGTGCAGCAGAGGACGAGGAATTGGGTTCCAAACAAGACATGCAAGACTGGTTGCTGAGTGTTGGTATTATATCGCCTGTCACCAAAGAGTCTGCTGGTGCTCTTTACCACCAACAATTGTCCCGTCAGTTGGCAGATTTTGTCAGGCTTCCACTTGAGAGAGCTGGAGGAATGATGAATCTTATAGATATTTACTGTCTCTTCAATCGTGCTCGAGGAACAGAATTGATCTCACCAGAGGATCTGCTGCAAGCATGTTTTCTTTGGGAGAAGTTTGATGTCCCAGTAATGCTTCGAAAGTTTGATAGTGGGGTGATGGTAATCCAGAATAAGTCCCACAGTGATGAAGAGATTTTTGCTAGGATCAAGACCCTTGCAACAAAGCCCGATGCTCTTCGAACTGGTATAAGTGCCAGTGATGCTGCAATCACACTAGGTATTGCTCCAGGCATGGCCAAGGAGCATCTTCTAACTGCTGAAAGCAAAGGTTTTCTATGCAGGGATATTAGCCCGGATGGCTTTCGCTTTTATATTAACCTGTTTCCAGACATTGATCCAAACAATGTTTACTTAGTGAAAGATTATGGAGTCTATGACACATGGTTAAAGGTGGCCTCTGCTTCTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000814 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007033 GO:0007034 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010008 GO:0010154 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0019538 GO:0019941 GO:0022414 GO:0030163 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032509 GO:0032511 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0036452 GO:0042886 GO:0043130 GO:0043162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043328 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045324 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0061458 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071944 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0090351 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EAP30 Vps36_ESCRT-II

Protein Analysis

444

Amino Acids

48.61

Weight (kDa)

5.48

Isoelectric Point (pI)

42.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps36_ESCRT-II	PF11605	10 - 100	5.8e-13	Vacuolar protein sorting protein 36 Vps36
EAP30	PF04157	179 - 403	2.2e-56	EAP30/Vps36 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04920
fragaria_vesca	FvH4_4g11870 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040 FvH4_7g05040
malus_domestica	MD02G1263600.v1.1 MD07G1054600.v1.1
prunus_persica	Prupe.2G061300_v2.0.a1 Prupe.2G061300_v2.0.a1 Prupe.2G061300_v2.0.a1
pyrus_communis	pycom02g22580 pycom07g04240
rosa_chinensis	RchiOBHm_Chr1g0331361
rosa_laevigata	RLG00000029741
rosa_multiflora	Rmu_sc0005956.1_g000008
rosa_roxburghii	Rroxscaffold_4G00319220
rosa_rugosa	Rorug01G0092800
rosa_samantha	Rh1AG117800 Rh1BG088500 Rh1CG112700 Rh1DG121600
rosa_wichuraiana	Rw1G009420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	414
AccI	GTMKAC	2 cut(s)	96, 491
AciI	CCGC	3 cut(s)	166, 246, 427
AclWI	GGATC	5 cut(s)	280, 293, 954, 1076, 1253
AcoI	YGGCCR	3 cut(s)	3, 316, 1158
AcsI	RAATTY	1 cut(s)	328
AcuI	CTGAAG	1 cut(s)	192
AcyI	GRCGYC	1 cut(s)	46
AfiI	CCNNNNNNNGG	8 cut(s)	139, 175, 282, 288, 301, 318, 414, 835
AflIII	ACRYGT	1 cut(s)	396
AgsI	TTSAA	4 cut(s)	74, 327, 619, 910
AhdI	GACNNNNNGTC	1 cut(s)	50
AjiI	CACGTC	1 cut(s)	397
AjnI	CCWGG	4 cut(s)	437, 609, 703, 1150
AluBI	AGCT	6 cut(s)	23, 31, 298, 594, 638, 869
AluI	AGCT	6 cut(s)	23, 31, 298, 594, 638, 869
Alw21I	GWGCWC	2 cut(s)	808, 919
Alw26I	GTCTC	4 cut(s)	184, 437, 572, 908
AlwI	GGATC	5 cut(s)	280, 293, 954, 1076, 1253
Ama87I	CYCGRG	1 cut(s)	918
AoxI	GGCC	4 cut(s)	3, 316, 1158, 1317
ApeKI	GCWGC	3 cut(s)	710, 952, 1127
ApoI	RAATTY	1 cut(s)	328
AspS9I	GGNCC	1 cut(s)	348
AsuC2I	CCSGG	3 cut(s)	283, 320, 1217
AsuHPI	GGTGA	7 cut(s)	142, 300, 538, 661, 778, 930, 1030
AsuII	TTCGAA	2 cut(s)	1000, 1102
AsuNHI	GCTAGC	1 cut(s)	655
AvaI	CYCGRG	1 cut(s)	918
AvaII	GGWCC	1 cut(s)	348
BalI	TGGCCA	1 cut(s)	1160
BamHI	GGATCC	1 cut(s)	285
BbsI	GAAGAC	1 cut(s)	117
Bbv12I	GWGCWC	2 cut(s)	808, 919
BbvI	GCAGC	3 cut(s)	722, 939, 1114
BccI	CCATC	9 cut(s)	70, 221, 236, 275, 359, 526, 640, 1015, 1214
BceAI	ACGGC	1 cut(s)	331
BciT130I	CCWGG	4 cut(s)	439, 611, 705, 1152
BciVI	GTATCC	1 cut(s)	155
BcnI	CCSGG	3 cut(s)	283, 320, 1217
BcoDI	GTCTC	4 cut(s)	184, 437, 572, 908
BfaI	CTAG	5 cut(s)	345, 656, 1065, 1139, 1329
BfmI	CTRYAG	1 cut(s)	93
BfuI	GTATCC	1 cut(s)	155
BglI	GCCNNNNNGGC	1 cut(s)	1221
BisI	GCNGC	3 cut(s)	711, 953, 1128
BlsI	GCNGC	3 cut(s)	712, 954, 1129
Bme1390I	CCNGG	7 cut(s)	283, 320, 439, 611, 705, 1152, 1217
Bme18I	GGWCC	1 cut(s)	348
BmeRI	GACNNNNNGTC	1 cut(s)	50
BmeT110I	CYCGRG	1 cut(s)	918
BmgBI	CACGTC	1 cut(s)	397
BmgT120I	GGNCC	1 cut(s)	348
BmiI	GGNNCC	4 cut(s)	202, 287, 293, 733
BmrFI	CCNGG	7 cut(s)	283, 320, 439, 611, 705, 1152, 1217
BmrI	ACTGGG	3 cut(s)	44, 456, 983
BmsI	GCATC	5 cut(s)	592, 642, 1084, 1114, 1177
BmtI	GCTAGC	1 cut(s)	659
BmuI	ACTGGG	3 cut(s)	44, 456, 983
BpiI	GAAGAC	1 cut(s)	117
BpmI	CTGGAG	2 cut(s)	891, 1134
Bpu14I	TTCGAA	2 cut(s)	1000, 1102
BpuEI	CTTGAG	2 cut(s)	245, 881
BpuMI	CCSGG	3 cut(s)	283, 320, 1217
BsaBI	GATNNNNATC	1 cut(s)	1025
BsaHI	GRCGYC	1 cut(s)	46
BsaI	GGTCTC	1 cut(s)	184
BsaJI	CCNNGG	4 cut(s)	281, 438, 513, 1161
BsaWI	WCCGGW	1 cut(s)	288
BsaXI	ACNNNNNCTCC	2 cut(s)	227, 257
Bsc4I	CCNNNNNNNGG	8 cut(s)	139, 175, 282, 288, 301, 318, 414, 835
Bse1I	ACTGG	7 cut(s)	50, 428, 451, 758, 989, 1111, 1119
Bse8I	GATNNNNATC	1 cut(s)	1025
BseBI	CCWGG	4 cut(s)	439, 611, 705, 1152
BseDI	CCNNGG	4 cut(s)	281, 438, 513, 1161
BseGI	GGATG	4 cut(s)	213, 537, 607, 1225
BseJI	GATNNNNATC	1 cut(s)	1025
BseLI	CCNNNNNNNGG	8 cut(s)	139, 175, 282, 288, 301, 318, 414, 835
BseMII	CTCAG	4 cut(s)	465, 568, 713, 752
BseNI	ACTGG	7 cut(s)	50, 428, 451, 758, 989, 1111, 1119
BseRI	GAGGAG	3 cut(s)	47, 74, 178
BseX3I	CGGCCG	1 cut(s)	316
BseXI	GCAGC	3 cut(s)	722, 939, 1114
BsgI	GTGCAG	1 cut(s)	729
Bsh1285I	CGRYCG	1 cut(s)	319
BshFI	GGCC	4 cut(s)	5, 318, 1160, 1319
BsiEI	CGRYCG	1 cut(s)	319
BsiHKAI	GWGCWC	2 cut(s)	808, 919
BsiHKCI	CYCGRG	1 cut(s)	918
BsiSI	CCGG	6 cut(s)	6, 283, 289, 319, 687, 1217
BslFI	GGGAC	4 cut(s)	241, 811, 971, 1024
BslI	CCNNNNNNNGG	8 cut(s)	139, 175, 282, 288, 301, 318, 414, 835
BsmAI	GTCTC	4 cut(s)	184, 437, 572, 908
BsmFI	GGGAC	4 cut(s)	241, 811, 971, 1024
BsmI	GAATGC	3 cut(s)	110, 457, 625
BsnI	GGCC	4 cut(s)	5, 318, 1160, 1319
Bso31I	GGTCTC	1 cut(s)	184
BsoBI	CYCGRG	1 cut(s)	918
Bsp119I	TTCGAA	2 cut(s)	1000, 1102
Bsp1286I	GDGCHC	2 cut(s)	808, 919
Bsp143I	GATC	5 cut(s)	285, 933, 946, 1068, 1258
BspACI	CCGC	3 cut(s)	166, 246, 427
BspANI	GGCC	4 cut(s)	5, 318, 1160, 1319
BspCNI	CTCAG	4 cut(s)	466, 567, 712, 753
BspHI	TCATGA	1 cut(s)	627
BspLI	GGNNCC	4 cut(s)	202, 287, 293, 733
BspOI	GCTAGC	1 cut(s)	659
BspPI	GGATC	5 cut(s)	280, 293, 954, 1076, 1253
BspQI	GCTCTTC	2 cut(s)	427, 1104
BspT104I	TTCGAA	2 cut(s)	1000, 1102
BspTNI	GGTCTC	1 cut(s)	184
BsrI	ACTGG	7 cut(s)	50, 428, 451, 758, 989, 1111, 1119
BssECI	CCNNGG	4 cut(s)	281, 438, 513, 1161
BssMI	GATC	5 cut(s)	285, 933, 946, 1068, 1258
BssNI	GRCGYC	1 cut(s)	46
BssT1I	CCWWGG	2 cut(s)	513, 1161
Bst2UI	CCWGG	4 cut(s)	439, 611, 705, 1152
Bst4CI	ACNGT	5 cut(s)	94, 364, 526, 902, 1046
Bst6I	CTCTTC	6 cut(s)	196, 211, 427, 911, 1047, 1104
BstACI	GRCGYC	1 cut(s)	46
BstBI	TTCGAA	2 cut(s)	1000, 1102
BstC8I	GCNNGC	3 cut(s)	596, 657, 957
BstDEI	CTNAG	5 cut(s)	474, 554, 699, 761, 1276
BstF5I	GGATG	4 cut(s)	213, 537, 607, 1225
BstKTI	GATC	5 cut(s)	288, 936, 949, 1071, 1261
BstMAI	GTCTC	4 cut(s)	184, 437, 572, 908
BstMBI	GATC	5 cut(s)	285, 933, 946, 1068, 1258
BstMCI	CGRYCG	1 cut(s)	319
BstMWI	GCNNNNNNNGC	4 cut(s)	45, 209, 1124, 1221
BstNI	CCWGG	4 cut(s)	439, 611, 705, 1152
BstNSI	RCATGY	2 cut(s)	748, 963
BstSCI	CCNGG	7 cut(s)	281, 318, 437, 609, 703, 1150, 1215
BstSFI	CTRYAG	1 cut(s)	93
BstV1I	GCAGC	3 cut(s)	722, 939, 1114
BstV2I	GAAGAC	1 cut(s)	117
BstX2I	RGATCY	2 cut(s)	285, 946
BstYI	RGATCY	2 cut(s)	285, 946
BstZI	CGGCCG	1 cut(s)	316
BsuI	GTATCC	1 cut(s)	155
BsuRI	GGCC	4 cut(s)	5, 318, 1160, 1319
BtrI	CACGTC	1 cut(s)	397
BtsCI	GGATG	4 cut(s)	213, 537, 607, 1225
BtsI	GCAGTG	1 cut(s)	46
BtsIMutI	CAGTG	5 cut(s)	46, 90, 369, 1051, 1126
Cac8I	GCNNGC	3 cut(s)	596, 657, 957
CciI	TCATGA	1 cut(s)	627
Cfr13I	GGNCC	1 cut(s)	348
CseI	GACGC	1 cut(s)	54
CspCI	CAANNNNNGTGG	2 cut(s)	554, 589
CviAII	CATG	5 cut(s)	628, 745, 960, 1156, 1305
DdeI	CTNAG	5 cut(s)	474, 554, 699, 761, 1276
DpnI	GATC	5 cut(s)	287, 935, 948, 1070, 1260
DpnII	GATC	5 cut(s)	285, 933, 946, 1068, 1258
DriI	GACNNNNNGTC	1 cut(s)	50
EaeI	YGGCCR	3 cut(s)	3, 316, 1158
EagI	CGGCCG	1 cut(s)	316
Eam1104I	CTCTTC	6 cut(s)	196, 211, 427, 911, 1047, 1104
Eam1105I	GACNNNNNGTC	1 cut(s)	50
EarI	CTCTTC	6 cut(s)	196, 211, 427, 911, 1047, 1104
EciI	GGCGGA	1 cut(s)	235
EclXI	CGGCCG	1 cut(s)	316
Eco130I	CCWWGG	2 cut(s)	513, 1161
Eco31I	GGTCTC	1 cut(s)	184
Eco47I	GGWCC	1 cut(s)	348
Eco52I	CGGCCG	1 cut(s)	316
Eco57I	CTGAAG	1 cut(s)	192
Eco88I	CYCGRG	1 cut(s)	918
EcoRII	CCWGG	4 cut(s)	437, 609, 703, 1150
EcoT14I	CCWWGG	2 cut(s)	513, 1161
ErhI	CCWWGG	2 cut(s)	513, 1161
FaeI	CATG	5 cut(s)	631, 748, 963, 1159, 1308
FalI	AAGNNNNNCTT	2 cut(s)	254, 286
FaqI	GGGAC	4 cut(s)	241, 811, 971, 1024
FatI	CATG	5 cut(s)	627, 744, 959, 1155, 1304
FauI	CCCGC	1 cut(s)	420
FblI	GTMKAC	2 cut(s)	96, 491
Fnu4HI	GCNGC	3 cut(s)	711, 953, 1128
FokI	GGATG	4 cut(s)	200, 544, 614, 1232
Fsp4HI	GCNGC	3 cut(s)	711, 953, 1128
FspBI	CTAG	5 cut(s)	345, 656, 1065, 1139, 1329
GluI	GCNGC	3 cut(s)	711, 953, 1128
GsuI	CTGGAG	2 cut(s)	891, 1134
HaeIII	GGCC	4 cut(s)	5, 318, 1160, 1319
HapII	CCGG	6 cut(s)	6, 283, 289, 319, 687, 1217
HgaI	GACGC	1 cut(s)	54
Hin1I	GRCGYC	1 cut(s)	46
Hin1II	CATG	5 cut(s)	631, 748, 963, 1159, 1308
HincII	GTYRAC	1 cut(s)	492
HindII	GTYRAC	1 cut(s)	492
HinfI	GANTC	4 cut(s)	185, 794, 883, 1293
HpaII	CCGG	6 cut(s)	6, 283, 289, 319, 687, 1217
HphI	GGTGA	7 cut(s)	142, 300, 538, 661, 778, 930, 1030
Hpy166II	GTNNAC	4 cut(s)	44, 97, 492, 1273
Hpy188I	TCNGA	3 cut(s)	67, 184, 475
Hpy188III	TCNNGA	8 cut(s)	501, 546, 556, 628, 1030, 1072, 1250, 1329
Hpy8I	GTNNAC	4 cut(s)	44, 97, 492, 1273
Hpy99I	CGWCG	2 cut(s)	317, 359
HpyAV	CCTTC	3 cut(s)	373, 512, 553
HpyCH4III	ACNGT	5 cut(s)	94, 364, 526, 902, 1046
HpyCH4IV	ACGT	1 cut(s)	396
HpyCH4V	TGCA	8 cut(s)	455, 598, 710, 748, 955, 1082, 1130, 1203
HpyF10VI	GCNNNNNNNGC	4 cut(s)	45, 209, 1124, 1221
HpyF3I	CTNAG	5 cut(s)	474, 554, 699, 761, 1276
HpySE526I	ACGT	1 cut(s)	396
Hsp92I	GRCGYC	1 cut(s)	46
Hsp92II	CATG	5 cut(s)	631, 748, 963, 1159, 1308
Kzo9I	GATC	5 cut(s)	285, 933, 946, 1068, 1258
LguI	GCTCTTC	2 cut(s)	427, 1104
LmnI	GCTCC	5 cut(s)	28, 87, 200, 1153, 1165
Lsp1109I	GCAGC	3 cut(s)	722, 939, 1114
LweI	GCATC	5 cut(s)	592, 642, 1084, 1114, 1177
MaeI	CTAG	5 cut(s)	345, 656, 1065, 1139, 1329
MaeII	ACGT	1 cut(s)	396
MaeIII	GTNAC	4 cut(s)	306, 358, 460, 784
MalI	GATC	5 cut(s)	287, 935, 948, 1070, 1260
MboI	GATC	5 cut(s)	285, 933, 946, 1068, 1258
MfeI	CAATTG	1 cut(s)	821
MflI	RGATCY	2 cut(s)	285, 946
MhlI	GDGCHC	2 cut(s)	808, 919
MlsI	TGGCCA	1 cut(s)	1160
MluCI	AATT	5 cut(s)	10, 328, 725, 821, 929
MluNI	TGGCCA	1 cut(s)	1160
MlyI	GAGTC	3 cut(s)	179, 803, 1302
MmeI	TCCRAC	1 cut(s)	207
Mox20I	TGGCCA	1 cut(s)	1160
MscI	TGGCCA	1 cut(s)	1160
MseI	TTAA	2 cut(s)	1239, 1310
MslI	CAYNNNNRTG	1 cut(s)	1047
Msp20I	TGGCCA	1 cut(s)	1160
MspI	CCGG	6 cut(s)	6, 283, 289, 319, 687, 1217
MspR9I	CCNGG	7 cut(s)	283, 320, 439, 611, 705, 1152, 1217
MunI	CAATTG	1 cut(s)	821
Mva1269I	GAATGC	3 cut(s)	110, 457, 625
MvaI	CCWGG	4 cut(s)	439, 611, 705, 1152
MwoI	GCNNNNNNNGC	4 cut(s)	45, 209, 1124, 1221
NciI	CCSGG	3 cut(s)	283, 320, 1217
NdeII	GATC	5 cut(s)	285, 933, 946, 1068, 1258
NheI	GCTAGC	1 cut(s)	655
NlaIII	CATG	5 cut(s)	631, 748, 963, 1159, 1308
NlaIV	GGNNCC	4 cut(s)	202, 287, 293, 733
NmuCI	GTSAC	3 cut(s)	306, 460, 784
NspI	RCATGY	2 cut(s)	748, 963
NspV	TTCGAA	2 cut(s)	1000, 1102
PaeR7I	CTCGAG	1 cut(s)	918
PagI	TCATGA	1 cut(s)	627
PciSI	GCTCTTC	2 cut(s)	427, 1104
PctI	GAATGC	3 cut(s)	110, 457, 625
PfeI	GAWTC	1 cut(s)	883
PflMI	CCANNNNNTGG	1 cut(s)	414
PfoI	TCCNGGA	1 cut(s)	609
PkrI	GCNGC	3 cut(s)	712, 954, 1129
PleI	GAGTC	3 cut(s)	179, 802, 1301
PpsI	GAGTC	3 cut(s)	179, 802, 1301
Psp6I	CCWGG	4 cut(s)	437, 609, 703, 1150
PspGI	CCWGG	4 cut(s)	437, 609, 703, 1150
PspN4I	GGNNCC	4 cut(s)	202, 287, 293, 733
PspPI	GGNCC	1 cut(s)	348
PspXI	VCTCGAGB	1 cut(s)	918
PsuI	RGATCY	2 cut(s)	285, 946
RseI	CAYNNNNRTG	1 cut(s)	1047
SalI	GTCGAC	1 cut(s)	490
SapI	GCTCTTC	2 cut(s)	427, 1104
SaqAI	TTAA	2 cut(s)	1239, 1310
SatI	GCNGC	3 cut(s)	711, 953, 1128
Sau3AI	GATC	5 cut(s)	285, 933, 946, 1068, 1258
Sau96I	GGNCC	1 cut(s)	348
SchI	GAGTC	3 cut(s)	179, 803, 1302
ScrFI	CCNGG	7 cut(s)	283, 320, 439, 611, 705, 1152, 1217
SduI	GDGCHC	2 cut(s)	808, 919
SfaNI	GCATC	5 cut(s)	592, 642, 1084, 1114, 1177
SfcI	CTRYAG	1 cut(s)	93
Sfr274I	CTCGAG	1 cut(s)	918
SfuI	TTCGAA	2 cut(s)	1000, 1102
SinI	GGWCC	1 cut(s)	348
SlaI	CTCGAG	1 cut(s)	918
SmiMI	CAYNNNNRTG	1 cut(s)	1047
SmlI	CTYRAG	3 cut(s)	260, 860, 918
SmoI	CTYRAG	3 cut(s)	260, 860, 918
Sse9I	AATT	5 cut(s)	10, 328, 725, 821, 929
SsiI	CCGC	3 cut(s)	166, 246, 427
SspMI	CTAG	5 cut(s)	345, 656, 1065, 1139, 1329
StyD4I	CCNGG	7 cut(s)	281, 318, 437, 609, 703, 1150, 1215
StyI	CCWWGG	2 cut(s)	513, 1161
TaaI	ACNGT	5 cut(s)	94, 364, 526, 902, 1046
TaiI	ACGT	1 cut(s)	399
TaqI	TCGA	6 cut(s)	102, 491, 692, 919, 1000, 1102
TasI	AATT	5 cut(s)	10, 328, 725, 821, 929
TfiI	GAWTC	1 cut(s)	883
Tru1I	TTAA	2 cut(s)	1239, 1310
Tru9I	TTAA	2 cut(s)	1239, 1310
TscAI	CASTG	5 cut(s)	46, 97, 369, 1051, 1126
TseFI	GTSAC	3 cut(s)	306, 460, 784
TseI	GCWGC	3 cut(s)	710, 952, 1127
Tsp45I	GTSAC	3 cut(s)	306, 460, 784
TspDTI	ATGAA	2 cut(s)	896, 1065
TspRI	CASTG	5 cut(s)	46, 97, 369, 1051, 1126
Van91I	CCANNNNNTGG	1 cut(s)	414
VpaK11BI	GGWCC	1 cut(s)	348
XapI	RAATTY	1 cut(s)	328
XbaI	TCTAGA	1 cut(s)	1328
XceI	RCATGY	2 cut(s)	748, 963
XhoI	CTCGAG	1 cut(s)	918
XmiI	GTMKAC	2 cut(s)	96, 491
XspI	CTAG	5 cut(s)	345, 656, 1065, 1139, 1329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.