Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007723

Physical Location & Seq

Genomic Coordinates Forward (+)

5428591 .. 5431873

Gene Structure 3283 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1058500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGAGTCACAGAGGATTAATATATAGCTTTGTTGCAAAAGGAAGTGTTGTTTTAGCAGAACACACGACCTTCACCGGAAACTTTAGTACCATTGCTGTTCAATGCTTGCAGAAGCTACCTTCCAGCAGCAGCAAGTACACCTACTCGTGCGATGGCTACACTTTTAACTTCCTACTTGACAGTGGATTCGTGTTTCTTGTTGTTGCTGATGAATCGGCCGGAAGGAGTGTGCCTTTTGTGTTCCTTGAACGAGTGAAAGAGGATTTCAAGCAGCGGTATGCTGCAGATATTAAGAATGCAGGGCCGCATCCTCTTGATGATGATGATGAAGAGGATGACTTATTTGAAGATCGATTTAGCATCGCTTACAATCTTGACAGAGAATTTGGGCCAATGCTTAAGGAGCACATGCAATATTGCATGGAGCATCCAGAAGAAATAAGTAAGCTTTCCAAATTGAAGGCCCAAATCACGGAGGTGAAAGGTGTGATGATGGACAATATAGAGAAGGTTTTGGATCGCGGTGAGAGAATTGAGCTTTTGGTTGATAAAACAGAAAATCTGCAGTTCCAGGCTGACAGCTTCCAGAGGCAAGGCAGGCAATTGCGACGTAAGATGTGGCTGCAGAGTCTCCAAATGAAGCTAATGATTGGAGGTGGAGTCCTTATCCTGATCATCATAATATGGCTTATCGCCTGCGGAGGTTTCAAATGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006887 GO:0006906 GO:0006996 GO:0007033 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0031201 GO:0031410 GO:0031982 GO:0032940 GO:0032991 GO:0033036 GO:0033263 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046903 GO:0046907 GO:0048284 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090174 GO:0097708 GO:0098796 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Longin MMtag Synaptobrevin

Protein Analysis

239

Amino Acids

27.22

Weight (kDa)

5.46

Isoelectric Point (pI)

45.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Longin	PF13774	32 - 103	2e-19	Regulated-SNARE-like domain
Synaptobrevin	PF00957	149 - 229	4.4e-30	Synaptobrevin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007723)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54300 AT3G54300 AT3G54300
fragaria_vesca	FvH4_7g05140
malus_domestica	MD02G1261900.v1.1 MD07G1058500.v1.1
prunus_persica	Prupe.2G070100_v2.0.a1 Prupe.2G070100_v2.0.a1 Prupe.2G070100_v2.0.a1 Prupe.2G070100_v2.0.a1
pyrus_communis	pycom02g22390 pycom07g04470
rosa_chinensis	RchiOBHm_Chr1g0331711
rosa_laevigata	RLG00000029720
rosa_multiflora	Rmu_sc0005044.1_g000001 Rmu_sc0034229.1_g000001
rosa_roxburghii	Rroxscaffold_4G00318860
rosa_rugosa	Rorug01G0095900 Rorug01G0095900
rosa_samantha	Rh1AG119500 Rh1BG091100 Rh1CG114600 Rh1DG125000
rosa_wichuraiana	Rw1G009660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	522
AciI	CCGC	4 cut(s)	274, 305, 522, 699
AclWI	GGATC	1 cut(s)	525
AcoI	YGGCCR	1 cut(s)	216
AcsI	RAATTY	1 cut(s)	383
AfaI	GTAC	2 cut(s)	88, 137
AfiI	CCNNNNNNNGG	1 cut(s)	472
AflII	CTTAAG	1 cut(s)	398
AgsI	TTSAA	6 cut(s)	101, 248, 268, 347, 460, 709
AjnI	CCWGG	1 cut(s)	570
AleI	CACNNNNGTG	1 cut(s)	476
AluBI	AGCT	6 cut(s)	27, 115, 448, 538, 582, 643
AluI	AGCT	6 cut(s)	27, 115, 448, 538, 582, 643
Alw21I	GWGCWC	1 cut(s)	408
Alw26I	GTCTC	1 cut(s)	635
AlwI	GGATC	1 cut(s)	525
AoxI	GGCC	4 cut(s)	216, 302, 389, 462
ApeKI	GCWGC	5 cut(s)	126, 129, 271, 281, 622
ApoI	RAATTY	1 cut(s)	383
ArsI	GACNNNNNNTTYG	4 cut(s)	170, 202, 368, 400
AseI	ATTAAT	1 cut(s)	17
AspS9I	GGNCC	3 cut(s)	302, 389, 463
AsuHPI	GGTGA	3 cut(s)	64, 490, 536
BaeI	ACNNNNGTAYC	2 cut(s)	70, 103
BauI	CACGAG	1 cut(s)	145
Bbv12I	GWGCWC	1 cut(s)	408
BbvI	GCAGC	5 cut(s)	138, 141, 268, 283, 609
BccI	CCATC	2 cut(s)	146, 487
BciT130I	CCWGG	1 cut(s)	572
BclI	TGATCA	1 cut(s)	672
BcoDI	GTCTC	1 cut(s)	635
BfmI	CTRYAG	3 cut(s)	282, 563, 623
BfrI	CTTAAG	1 cut(s)	398
BisI	GCNGC	6 cut(s)	127, 130, 272, 282, 305, 623
BlsI	GCNGC	6 cut(s)	128, 131, 273, 283, 306, 624
Bme1390I	CCNGG	1 cut(s)	572
BmgT120I	GGNCC	3 cut(s)	302, 389, 463
BmrFI	CCNGG	1 cut(s)	572
BmsI	GCATC	3 cut(s)	316, 369, 436
Bsa29I	ATCGAT	1 cut(s)	352
BsaWI	WCCGGW	1 cut(s)	74
BsaXI	ACNNNNNCTCC	2 cut(s)	645, 675
Bsc4I	CCNNNNNNNGG	1 cut(s)	472
Bse3DI	GCAATG	1 cut(s)	90
BseBI	CCWGG	1 cut(s)	572
BseCI	ATCGAT	1 cut(s)	352
BseGI	GGATG	3 cut(s)	307, 340, 427
BseLI	CCNNNNNNNGG	1 cut(s)	472
BseMI	GCAATG	1 cut(s)	90
BseX3I	CGGCCG	1 cut(s)	216
BseXI	GCAGC	5 cut(s)	138, 141, 268, 283, 609
Bsh1236I	CGCG	1 cut(s)	522
Bsh1285I	CGRYCG	1 cut(s)	219
BshFI	GGCC	4 cut(s)	218, 304, 391, 464
BshVI	ATCGAT	1 cut(s)	352
BsiEI	CGRYCG	1 cut(s)	219
BsiHKAI	GWGCWC	1 cut(s)	408
BsiSI	CCGG	2 cut(s)	75, 219
BslI	CCNNNNNNNGG	1 cut(s)	472
BsmAI	GTCTC	1 cut(s)	635
BsmI	GAATGC	1 cut(s)	301
BsnI	GGCC	4 cut(s)	218, 304, 391, 464
Bsp1286I	GDGCHC	1 cut(s)	408
Bsp143I	GATC	3 cut(s)	349, 517, 672
BspACI	CCGC	4 cut(s)	274, 305, 522, 699
BspANI	GGCC	4 cut(s)	218, 304, 391, 464
BspDI	ATCGAT	1 cut(s)	352
BspFNI	CGCG	1 cut(s)	522
BspMAI	CTGCAG	3 cut(s)	286, 567, 627
BspPI	GGATC	1 cut(s)	525
BspTI	CTTAAG	1 cut(s)	398
BsrDI	GCAATG	1 cut(s)	90
BssMI	GATC	3 cut(s)	349, 517, 672
BssSI	CACGAG	1 cut(s)	145
Bst2BI	CACGAG	1 cut(s)	145
Bst2UI	CCWGG	1 cut(s)	572
Bst4CI	ACNGT	1 cut(s)	182
Bst6I	CTCTTC	1 cut(s)	324
BstAFI	CTTAAG	1 cut(s)	398
BstC8I	GCNNGC	3 cut(s)	107, 599, 697
BstF5I	GGATG	3 cut(s)	307, 340, 427
BstFNI	CGCG	1 cut(s)	522
BstKTI	GATC	3 cut(s)	352, 520, 675
BstMAI	GTCTC	1 cut(s)	635
BstMBI	GATC	3 cut(s)	349, 517, 672
BstMCI	CGRYCG	1 cut(s)	219
BstMWI	GCNNNNNNNGC	2 cut(s)	403, 598
BstNI	CCWGG	1 cut(s)	572
BstNSI	RCATGY	1 cut(s)	412
BstSCI	CCNGG	1 cut(s)	570
BstSFI	CTRYAG	3 cut(s)	282, 563, 623
BstUI	CGCG	1 cut(s)	522
BstV1I	GCAGC	5 cut(s)	138, 141, 268, 283, 609
BstZI	CGGCCG	1 cut(s)	216
Bsu15I	ATCGAT	1 cut(s)	352
BsuRI	GGCC	4 cut(s)	218, 304, 391, 464
BsuTUI	ATCGAT	1 cut(s)	352
BtgZI	GCGATG	2 cut(s)	165, 346
BtsCI	GGATG	3 cut(s)	307, 340, 427
BtsIMutI	CAGTG	1 cut(s)	187
Cac8I	GCNNGC	3 cut(s)	107, 599, 697
Cfr13I	GGNCC	3 cut(s)	302, 389, 463
ClaI	ATCGAT	1 cut(s)	352
Csp6I	GTAC	2 cut(s)	87, 136
CviAII	CATG	2 cut(s)	409, 421
CviQI	GTAC	2 cut(s)	87, 136
DpnI	GATC	3 cut(s)	351, 519, 674
DpnII	GATC	3 cut(s)	349, 517, 672
EaeI	YGGCCR	1 cut(s)	216
EagI	CGGCCG	1 cut(s)	216
Eam1104I	CTCTTC	1 cut(s)	324
EarI	CTCTTC	1 cut(s)	324
EclXI	CGGCCG	1 cut(s)	216
Eco52I	CGGCCG	1 cut(s)	216
EcoRII	CCWGG	1 cut(s)	570
FaeI	CATG	2 cut(s)	412, 424
FaiI	YATR	8 cut(s)	22, 24, 279, 410, 422, 503, 680, 685
FatI	CATG	2 cut(s)	408, 420
FbaI	TGATCA	1 cut(s)	672
Fnu4HI	GCNGC	6 cut(s)	127, 130, 272, 282, 305, 623
FokI	GGATG	3 cut(s)	294, 347, 414
Fsp4HI	GCNGC	6 cut(s)	127, 130, 272, 282, 305, 623
GluI	GCNGC	6 cut(s)	127, 130, 272, 282, 305, 623
HaeIII	GGCC	4 cut(s)	218, 304, 391, 464
HapII	CCGG	2 cut(s)	75, 219
Hin1II	CATG	2 cut(s)	412, 424
HindIII	AAGCTT	1 cut(s)	446
HinfI	GANTC	5 cut(s)	4, 186, 212, 628, 660
HpaII	CCGG	2 cut(s)	75, 219
HphI	GGTGA	3 cut(s)	64, 490, 536
Hpy166II	GTNNAC	1 cut(s)	138
Hpy188III	TCNNGA	5 cut(s)	314, 374, 431, 586, 670
Hpy8I	GTNNAC	1 cut(s)	138
Hpy99I	CGWCG	1 cut(s)	612
HpyAV	CCTTC	5 cut(s)	79, 129, 216, 454, 502
HpyCH4III	ACNGT	1 cut(s)	182
HpyCH4IV	ACGT	1 cut(s)	610
HpyCH4V	TGCA	8 cut(s)	35, 109, 284, 299, 412, 420, 565, 625
HpyF10VI	GCNNNNNNNGC	2 cut(s)	403, 598
HpySE526I	ACGT	1 cut(s)	610
Hsp92II	CATG	2 cut(s)	412, 424
Ksp22I	TGATCA	1 cut(s)	672
Kzo9I	GATC	3 cut(s)	349, 517, 672
LmnI	GCTCC	2 cut(s)	403, 424
Lsp1109I	GCAGC	5 cut(s)	138, 141, 268, 283, 609
LweI	GCATC	3 cut(s)	316, 369, 436
MaeII	ACGT	1 cut(s)	610
MaeIII	GTNAC	1 cut(s)	5
MalI	GATC	3 cut(s)	351, 519, 674
MboI	GATC	3 cut(s)	349, 517, 672
MboII	GAAGA	3 cut(s)	341, 359, 446
MfeI	CAATTG	1 cut(s)	602
MhlI	GDGCHC	1 cut(s)	408
MluCI	AATT	4 cut(s)	383, 455, 531, 602
MlyI	GAGTC	3 cut(s)	13, 637, 669
MnlI	CCTC	8 cut(s)	5, 253, 321, 325, 469, 582, 647, 695
MseI	TTAA	4 cut(s)	17, 165, 291, 399
MslI	CAYNNNNRTG	1 cut(s)	476
MspA1I	CMGCKG	1 cut(s)	274
MspCI	CTTAAG	1 cut(s)	398
MspI	CCGG	2 cut(s)	75, 219
MspR9I	CCNGG	1 cut(s)	572
MunI	CAATTG	1 cut(s)	602
Mva1269I	GAATGC	1 cut(s)	301
MvaI	CCWGG	1 cut(s)	572
MvnI	CGCG	1 cut(s)	522
MwoI	GCNNNNNNNGC	2 cut(s)	403, 598
NdeII	GATC	3 cut(s)	349, 517, 672
NlaIII	CATG	2 cut(s)	412, 424
NmuCI	GTSAC	1 cut(s)	5
NspI	RCATGY	1 cut(s)	412
OliI	CACNNNNGTG	1 cut(s)	476
PctI	GAATGC	1 cut(s)	301
PfeI	GAWTC	2 cut(s)	186, 212
PkrI	GCNGC	6 cut(s)	128, 131, 273, 283, 306, 624
PleI	GAGTC	3 cut(s)	12, 636, 668
PpsI	GAGTC	3 cut(s)	12, 636, 668
PshBI	ATTAAT	1 cut(s)	17
Psp6I	CCWGG	1 cut(s)	570
PspGI	CCWGG	1 cut(s)	570
PspPI	GGNCC	3 cut(s)	302, 389, 463
PstI	CTGCAG	3 cut(s)	286, 567, 627
RsaI	GTAC	2 cut(s)	88, 137
RsaNI	GTAC	2 cut(s)	87, 136
RseI	CAYNNNNRTG	1 cut(s)	476
SaqAI	TTAA	4 cut(s)	17, 165, 291, 399
SatI	GCNGC	6 cut(s)	127, 130, 272, 282, 305, 623
Sau3AI	GATC	3 cut(s)	349, 517, 672
Sau96I	GGNCC	3 cut(s)	302, 389, 463
SchI	GAGTC	3 cut(s)	13, 637, 669
ScrFI	CCNGG	1 cut(s)	572
SduI	GDGCHC	1 cut(s)	408
SfaNI	GCATC	3 cut(s)	316, 369, 436
SfcI	CTRYAG	3 cut(s)	282, 563, 623
SmiMI	CAYNNNNRTG	1 cut(s)	476
SmlI	CTYRAG	1 cut(s)	398
SmoI	CTYRAG	1 cut(s)	398
Sse9I	AATT	4 cut(s)	383, 455, 531, 602
SsiI	CCGC	4 cut(s)	274, 305, 522, 699
SspI	AATATT	1 cut(s)	416
StyD4I	CCNGG	1 cut(s)	570
TaaI	ACNGT	1 cut(s)	182
TaiI	ACGT	1 cut(s)	613
TaqI	TCGA	1 cut(s)	352
TasI	AATT	4 cut(s)	383, 455, 531, 602
TatI	WGTACW	1 cut(s)	135
TauI	GCSGC	1 cut(s)	307
TfiI	GAWTC	2 cut(s)	186, 212
Tru1I	TTAA	4 cut(s)	17, 165, 291, 399
Tru9I	TTAA	4 cut(s)	17, 165, 291, 399
TscAI	CASTG	1 cut(s)	187
TseFI	GTSAC	1 cut(s)	5
TseI	GCWGC	5 cut(s)	126, 129, 271, 281, 622
Tsp45I	GTSAC	1 cut(s)	5
TspDTI	ATGAA	3 cut(s)	225, 342, 653
TspGWI	ACGGA	1 cut(s)	488
TspRI	CASTG	1 cut(s)	187
Vha464I	CTTAAG	1 cut(s)	398
VspI	ATTAAT	1 cut(s)	17
XapI	RAATTY	1 cut(s)	383
XceI	RCATGY	1 cut(s)	412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.