MD07G1077000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015249
Physical Location & Seq
Reverse (-)
7330328 .. 7330852
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1077000.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAGGAAAAGAAAGGAGATGCTAGAGTCTCTGTCATCTCCGGTCTCTTCTTCGTCTGCATTATCGCCGGCGGAGTATTTCTTTTCCTTTACATGCTTCTACCCGAAGAAAAAACCCAACCATGGTACCCCTTTGTAGGAATGGTGCTTGTGGCTATTCCATGGGCATTTTGGATCATGACATGTCTCTACAGATGCTTTAGGCCCGCCGGGGCCATGCATACCGGGGACAGCGGTCGCTATGCCAAGGCAGGCAGCAGCCGGGCAGCCACCATCCCCACCACGAGCGGCGCATTGACAGCTGGCAATGCATCATCTGCGGACTCCCCTTTTCATTCACCTGATGGTGACCGAAGGGTACAGTTTGCCGGGGTTGTTGTAATGGGGAACGAAGATGAGGGTGGACGCGGAGGCCAGAACGGAAACGACAACCATCATCAAGCCATAGACATGGAACATCATGATACGATCAATGATTCAGTTGGTTCTACGGATAGTGAAATGCCGTTGAGATTAATGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

18.8

Weight (kDa)

5.4

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17590
fragaria_vesca FvH4_7g07110
malus_domestica MD02G1239600.v1.1 MD07G1077000.v1.1
prunus_persica Prupe.2G098000_v2.0.a1
pyrus_communis pycom02g20520 pycom07g06050
rosa_chinensis RchiOBHm_Chr1g0337051
rosa_laevigata RLG00000029308
rosa_multiflora Rmu_sc0040337.1_g000001
rosa_roxburghii Rroxscaffold_4G00314990
rosa_rugosa Rorug01G0130800
rosa_samantha Rh1AG151500 Rh1BG120300 Rh1CG142700 Rh1DG158700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 288
AccII CGCG 1 cut(s) 408
AciI CCGC 6 cut(s) 72, 207, 234, 288, 320, 408
AclWI GGATC 1 cut(s) 182
AfaI GTAC 2 cut(s) 128, 360
AfiI CCNNNNNNNGG 2 cut(s) 123, 137
AflIII ACRYGT 1 cut(s) 182
AluBI AGCT 1 cut(s) 302
AluI AGCT 1 cut(s) 302
Alw26I GTCTC 3 cut(s) 34, 50, 191
AlwI GGATC 1 cut(s) 182
AoxI GGCC 3 cut(s) 203, 213, 412
ApeKI GCWGC 3 cut(s) 255, 258, 266
AseI ATTAAT 1 cut(s) 515
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 204, 213
AsuC2I CCSGG 4 cut(s) 211, 226, 263, 370
AsuHPI GGTGA 2 cut(s) 330, 359
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BanI GGYRCC 1 cut(s) 126
BauI CACGAG 1 cut(s) 283
BbvI GCAGC 3 cut(s) 267, 270, 278
BccI CCATC 3 cut(s) 281, 338, 441
BceAI ACGGC 1 cut(s) 490
BcnI CCSGG 4 cut(s) 211, 226, 263, 370
BcoDI GTCTC 3 cut(s) 34, 50, 191
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 190
BisI GCNGC 4 cut(s) 256, 259, 267, 289
BlsI GCNGC 4 cut(s) 257, 260, 268, 290
Bme1390I CCNGG 4 cut(s) 211, 226, 263, 370
BmgT120I GGNCC 2 cut(s) 204, 213
BmiI GGNNCC 2 cut(s) 128, 214
BmrFI CCNGG 4 cut(s) 211, 226, 263, 370
BmsI GCATC 3 cut(s) 10, 185, 320
BoxI GACNNNNGTC 1 cut(s) 234
BpuMI CCSGG 4 cut(s) 211, 226, 263, 370
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 6 cut(s) 122, 161, 210, 225, 246, 369
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 137
Bse118I RCCGGY 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 313
BseDI CCNNGG 6 cut(s) 122, 161, 210, 225, 246, 369
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 123, 137
BseMI GCAATG 1 cut(s) 313
BseXI GCAGC 3 cut(s) 267, 270, 278
Bsh1236I CGCG 1 cut(s) 408
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 205, 215, 414
BshNI GGYRCC 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 6 cut(s) 42, 69, 210, 225, 262, 369
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 123, 137
BsmAI GTCTC 3 cut(s) 34, 50, 191
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 3 cut(s) 205, 215, 414
Bso31I GGTCTC 1 cut(s) 50
Bsp143I GATC 2 cut(s) 174, 468
Bsp19I CCATGG 2 cut(s) 122, 161
BspACI CCGC 6 cut(s) 72, 207, 234, 288, 320, 408
BspANI GGCC 3 cut(s) 205, 215, 414
BspFNI CGCG 1 cut(s) 408
BspHI TCATGA 2 cut(s) 177, 460
BspLI GGNNCC 2 cut(s) 128, 214
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 50
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 6 cut(s) 122, 161, 210, 225, 246, 369
BssMI GATC 2 cut(s) 174, 468
BssSI CACGAG 1 cut(s) 283
BssT1I CCWWGG 3 cut(s) 122, 161, 246
Bst2BI CACGAG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 317
BstC8I GCNNGC 4 cut(s) 70, 207, 253, 304
BstDSI CCRYGG 2 cut(s) 122, 161
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 177, 471
BstMAI GTCTC 3 cut(s) 34, 50, 191
BstMBI GATC 2 cut(s) 174, 468
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 3 cut(s) 299, 308, 317
BstNSI RCATGY 2 cut(s) 97, 186
BstPAI GACNNNNGTC 1 cut(s) 234
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 4 cut(s) 209, 224, 261, 368
BstSFI CTRYAG 1 cut(s) 190
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 3 cut(s) 267, 270, 278
BstXI CCANNNNNNTGG 1 cut(s) 451
BsuRI GGCC 3 cut(s) 205, 215, 414
BtgI CCRYGG 2 cut(s) 122, 161
BtsCI GGATG 1 cut(s) 273
Cac8I GCNNGC 4 cut(s) 70, 207, 253, 304
CciI TCATGA 2 cut(s) 177, 460
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 204, 213
CseI GACGC 1 cut(s) 414
Csp6I GTAC 2 cut(s) 127, 359
CviAII CATG 8 cut(s) 94, 123, 162, 178, 183, 217, 451, 461
CviJI RGCY 8 cut(s) 155, 205, 215, 261, 269, 302, 414, 443
CviKI_1 RGCY 8 cut(s) 155, 205, 215, 261, 269, 302, 414, 443
CviQI GTAC 2 cut(s) 127, 359
DpnI GATC 2 cut(s) 176, 470
DpnII GATC 2 cut(s) 174, 468
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 3 cut(s) 122, 161, 246
Eco31I GGTCTC 1 cut(s) 50
Eco91I GGTNACC 1 cut(s) 347
EcoO65I GGTNACC 1 cut(s) 347
EcoT14I CCWWGG 3 cut(s) 122, 161, 246
EcoT22I ATGCAT 2 cut(s) 222, 313
ErhI CCWWGG 3 cut(s) 122, 161, 246
FaeI CATG 8 cut(s) 97, 126, 165, 181, 186, 220, 454, 464
FaqI GGGAC 1 cut(s) 242
FatI CATG 8 cut(s) 93, 122, 161, 177, 182, 216, 450, 460
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 4 cut(s) 256, 259, 267, 289
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 4 cut(s) 256, 259, 267, 289
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 292
GluI GCNGC 4 cut(s) 256, 259, 267, 289
HaeIII GGCC 3 cut(s) 205, 215, 414
HapII CCGG 6 cut(s) 42, 69, 210, 225, 262, 369
HgaI GACGC 1 cut(s) 414
HhaI GCGC 1 cut(s) 293
Hin1II CATG 8 cut(s) 97, 126, 165, 181, 186, 220, 454, 464
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 3 cut(s) 27, 323, 476
HpaII CCGG 6 cut(s) 42, 69, 210, 225, 262, 369
HphI GGTGA 2 cut(s) 330, 359
Hpy166II GTNNAC 1 cut(s) 404
Hpy188III TCNNGA 2 cut(s) 178, 461
Hpy8I GTNNAC 1 cut(s) 404
HpyAV CCTTC 1 cut(s) 348
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4V TGCA 3 cut(s) 60, 220, 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 308, 317
Hsp92II CATG 8 cut(s) 97, 126, 165, 181, 186, 220, 454, 464
HspAI GCGC 1 cut(s) 291
KpnI GGTACC 1 cut(s) 130
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 174, 468
Lsp1109I GCAGC 3 cut(s) 267, 270, 278
LweI GCATC 3 cut(s) 10, 185, 320
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 347
MalI GATC 2 cut(s) 176, 470
MbiI CCGCTC 1 cut(s) 288
MboI GATC 2 cut(s) 174, 468
MboII GAAGA 4 cut(s) 40, 43, 119, 404
MlyI GAGTC 2 cut(s) 36, 317
MnlI CCTC 2 cut(s) 391, 404
Mph1103I ATGCAT 2 cut(s) 222, 313
MreI CGCCGGCG 1 cut(s) 68
MroNI GCCGGC 1 cut(s) 68
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 1 cut(s) 449
MspA1I CMGCKG 2 cut(s) 234, 302
MspI CCGG 6 cut(s) 42, 69, 210, 225, 262, 369
MspR9I CCNGG 4 cut(s) 211, 226, 263, 370
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 3 cut(s) 299, 308, 317
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 4 cut(s) 211, 226, 263, 370
NcoI CCATGG 2 cut(s) 122, 161
NdeII GATC 2 cut(s) 174, 468
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 8 cut(s) 97, 126, 165, 181, 186, 220, 454, 464
NlaIV GGNNCC 2 cut(s) 128, 214
NmuCI GTSAC 1 cut(s) 347
NsiI ATGCAT 2 cut(s) 222, 313
NspI RCATGY 2 cut(s) 97, 186
PagI TCATGA 2 cut(s) 177, 460
PciI ACATGT 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 4 cut(s) 257, 260, 268, 290
PleI GAGTC 2 cut(s) 35, 317
PpsI GAGTC 2 cut(s) 35, 317
PscI ACATGT 1 cut(s) 182
PshAI GACNNNNGTC 1 cut(s) 234
PshBI ATTAAT 1 cut(s) 515
PspEI GGTNACC 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 128, 214
PspPI GGNCC 2 cut(s) 204, 213
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 128, 360
RsaNI GTAC 2 cut(s) 127, 359
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 4 cut(s) 256, 259, 267, 289
Sau3AI GATC 2 cut(s) 174, 468
Sau96I GGNCC 2 cut(s) 204, 213
SchI GAGTC 2 cut(s) 36, 317
ScrFI CCNGG 4 cut(s) 211, 226, 263, 370
SetI ASST 2 cut(s) 304, 343
SfaNI GCATC 3 cut(s) 10, 185, 320
SfcI CTRYAG 1 cut(s) 190
SgrAI CRCCGGYG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 449
SsiI CCGC 6 cut(s) 72, 207, 234, 288, 320, 408
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 4 cut(s) 209, 224, 261, 368
StyI CCWWGG 3 cut(s) 122, 161, 246
TaaI ACNGT 1 cut(s) 363
TaqII GACCGA 1 cut(s) 366
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TseFI GTSAC 1 cut(s) 347
TseI GCWGC 3 cut(s) 255, 258, 266
Tsp45I GTSAC 1 cut(s) 347
TspDTI ATGAA 1 cut(s) 323
TspGWI ACGGA 2 cut(s) 435, 506
VspI ATTAAT 1 cut(s) 515
XceI RCATGY 2 cut(s) 97, 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 168
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 2 cut(s) 222, 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.