pycom07g06050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015249
Physical Location & Seq
Reverse (-)
5352266 .. 5352793
528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06050.1

Sequence Viewer

Length: 528 bp
ATGGAGGAAAAGAAAGGAGATGCTAGAGTCTCTGTCATCTCCGGTCTCTTCTTCGTCTGCATTATCGCCGGCGGAGTATTTCTTTTCCTTTACATGCTTCTACCCGAAGAAAAAACCCAACCATGGTACCCCTTTGCAGGAATGGTGCTTGTGGCTATTCCATGGGCATTTTGGATCATGACATGTCTCTACAGATGCTTTAGGCCCGCGGGGGCCATGCATACCGGGGACAGCGGTCGCTATGCCAAGGCAGGCAGCAGCCGGGCAGCCACCATCCCCACCACGAGCGGCGCATTGACAGTTGGCAATGCTTCATCAGCTGCGGACTCCTCTGTTCATTCACCTAATGGTGACCGAAGGGTGCAGTTTGCCGGGGTGGTTGTAATGGGGAACGAAGATGAGGGTGGACGCAGAGGCCAGAACGGAAACGACAACCATTATCAAGCCATAGACATGGAACATCACAATACGATCAATGATTCAGTTGGTTCTACGGATAGTGAAATGCCGTTGAGATTAATGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

176

Amino Acids

18.94

Weight (kDa)

5.91

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015249)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G17590
fragaria_vesca FvH4_7g07110
malus_domestica MD02G1239600.v1.1 MD07G1077000.v1.1
prunus_persica Prupe.2G098000_v2.0.a1
pyrus_communis pycom02g20520 pycom07g06050
rosa_chinensis RchiOBHm_Chr1g0337051
rosa_laevigata RLG00000029308
rosa_multiflora Rmu_sc0040337.1_g000001
rosa_roxburghii Rroxscaffold_4G00314990
rosa_rugosa Rorug01G0130800
rosa_samantha Rh1AG151500 Rh1BG120300 Rh1CG142700 Rh1DG158700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 126
AccBSI CCGCTC 1 cut(s) 288
AccII CGCG 1 cut(s) 209
AciI CCGC 6 cut(s) 72, 207, 209, 234, 288, 323
AclWI GGATC 1 cut(s) 182
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 2 cut(s) 123, 137
AflIII ACRYGT 1 cut(s) 182
AluBI AGCT 1 cut(s) 320
AluI AGCT 1 cut(s) 320
Alw26I GTCTC 3 cut(s) 34, 50, 191
AlwI GGATC 1 cut(s) 182
AoxI GGCC 3 cut(s) 203, 213, 415
ApeKI GCWGC 4 cut(s) 255, 258, 266, 320
AseI ATTAAT 1 cut(s) 518
Asp718I GGTACC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 204, 213
AsuC2I CCSGG 3 cut(s) 226, 263, 373
AsuHPI GGTGA 2 cut(s) 333, 362
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BanI GGYRCC 1 cut(s) 126
BauI CACGAG 1 cut(s) 283
BbvI GCAGC 4 cut(s) 267, 270, 278, 307
BccI CCATC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 493
BcnI CCSGG 3 cut(s) 226, 263, 373
BcoDI GTCTC 3 cut(s) 34, 50, 191
BfaI CTAG 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 190
BisI GCNGC 5 cut(s) 256, 259, 267, 289, 321
BlsI GCNGC 5 cut(s) 257, 260, 268, 290, 322
Bme1390I CCNGG 3 cut(s) 226, 263, 373
BmgT120I GGNCC 2 cut(s) 204, 213
BmiI GGNNCC 2 cut(s) 128, 214
BmrFI CCNGG 3 cut(s) 226, 263, 373
BmsI GCATC 2 cut(s) 10, 185
BoxI GACNNNNGTC 1 cut(s) 234
BpuMI CCSGG 3 cut(s) 226, 263, 373
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 6 cut(s) 122, 161, 207, 225, 246, 372
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 137
Bse118I RCCGGY 1 cut(s) 68
Bse3DI GCAATG 1 cut(s) 313
BseDI CCNNGG 6 cut(s) 122, 161, 207, 225, 246, 372
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 123, 137
BseMI GCAATG 1 cut(s) 313
BseRI GAGGAG 1 cut(s) 319
BseXI GCAGC 4 cut(s) 267, 270, 278, 307
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 3 cut(s) 205, 215, 417
BshNI GGYRCC 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 5 cut(s) 42, 69, 225, 262, 372
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 123, 137
BsmAI GTCTC 3 cut(s) 34, 50, 191
BsmFI GGGAC 1 cut(s) 242
BsnI GGCC 3 cut(s) 205, 215, 417
Bso31I GGTCTC 1 cut(s) 50
Bsp143I GATC 2 cut(s) 174, 471
Bsp19I CCATGG 2 cut(s) 122, 161
BspACI CCGC 6 cut(s) 72, 207, 209, 234, 288, 323
BspANI GGCC 3 cut(s) 205, 215, 417
BspFNI CGCG 1 cut(s) 209
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 2 cut(s) 128, 214
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 50
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 68
BssAI RCCGGY 1 cut(s) 68
BssECI CCNNGG 6 cut(s) 122, 161, 207, 225, 246, 372
BssMI GATC 2 cut(s) 174, 471
BssSI CACGAG 1 cut(s) 283
BssT1I CCWWGG 3 cut(s) 122, 161, 246
Bst2BI CACGAG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 301
Bst6I CTCTTC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 70, 207, 253
BstDSI CCRYGG 3 cut(s) 122, 161, 207
BstEII GGTNACC 1 cut(s) 350
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 209
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 2 cut(s) 177, 474
BstMAI GTCTC 3 cut(s) 34, 50, 191
BstMBI GATC 2 cut(s) 174, 471
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNSI RCATGY 2 cut(s) 97, 186
BstPAI GACNNNNGTC 1 cut(s) 234
BstPI GGTNACC 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 224, 261, 371
BstSFI CTRYAG 1 cut(s) 190
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 4 cut(s) 267, 270, 278, 307
BstXI CCANNNNNNTGG 1 cut(s) 454
BsuRI GGCC 3 cut(s) 205, 215, 417
BtgI CCRYGG 3 cut(s) 122, 161, 207
BtsCI GGATG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 70, 207, 253
CciI TCATGA 1 cut(s) 177
CfoI GCGC 1 cut(s) 293
Cfr10I RCCGGY 1 cut(s) 68
Cfr13I GGNCC 2 cut(s) 204, 213
Cfr42I CCGCGG 1 cut(s) 210
CseI GACGC 1 cut(s) 417
Csp6I GTAC 1 cut(s) 127
CviAII CATG 7 cut(s) 94, 123, 162, 178, 183, 217, 454
CviJI RGCY 8 cut(s) 155, 205, 215, 261, 269, 320, 417, 446
CviKI_1 RGCY 8 cut(s) 155, 205, 215, 261, 269, 320, 417, 446
CviQI GTAC 1 cut(s) 127
DpnI GATC 2 cut(s) 176, 473
DpnII GATC 2 cut(s) 174, 471
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 87
Eco130I CCWWGG 3 cut(s) 122, 161, 246
Eco31I GGTCTC 1 cut(s) 50
Eco91I GGTNACC 1 cut(s) 350
EcoO65I GGTNACC 1 cut(s) 350
EcoT14I CCWWGG 3 cut(s) 122, 161, 246
EcoT22I ATGCAT 1 cut(s) 222
ErhI CCWWGG 3 cut(s) 122, 161, 246
FaeI CATG 7 cut(s) 97, 126, 165, 181, 186, 220, 457
FaqI GGGAC 1 cut(s) 242
FatI CATG 7 cut(s) 93, 122, 161, 177, 182, 216, 453
FauI CCCGC 2 cut(s) 202, 214
Fnu4HI GCNGC 5 cut(s) 256, 259, 267, 289, 321
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 5 cut(s) 256, 259, 267, 289, 321
FspBI CTAG 1 cut(s) 24
GlaI GCGC 1 cut(s) 292
GluI GCNGC 5 cut(s) 256, 259, 267, 289, 321
HaeIII GGCC 3 cut(s) 205, 215, 417
HapII CCGG 5 cut(s) 42, 69, 225, 262, 372
HgaI GACGC 1 cut(s) 417
HhaI GCGC 1 cut(s) 293
Hin1II CATG 7 cut(s) 97, 126, 165, 181, 186, 220, 457
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 3 cut(s) 27, 326, 479
HpaII CCGG 5 cut(s) 42, 69, 225, 262, 372
HphI GGTGA 2 cut(s) 333, 362
Hpy166II GTNNAC 1 cut(s) 407
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4V TGCA 4 cut(s) 60, 137, 220, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
Hsp92II CATG 7 cut(s) 97, 126, 165, 181, 186, 220, 457
HspAI GCGC 1 cut(s) 291
KpnI GGTACC 1 cut(s) 130
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 2 cut(s) 174, 471
LpnPI CCDG 8 cut(s) 55, 82, 123, 237, 238, 275, 385, 431
Lsp1109I GCAGC 4 cut(s) 267, 270, 278, 307
LweI GCATC 2 cut(s) 10, 185
MaeI CTAG 1 cut(s) 24
MaeIII GTNAC 1 cut(s) 350
MalI GATC 2 cut(s) 176, 473
MbiI CCGCTC 1 cut(s) 288
MboI GATC 2 cut(s) 174, 471
MboII GAAGA 4 cut(s) 40, 43, 119, 407
MlyI GAGTC 2 cut(s) 36, 320
MnlI CCTC 3 cut(s) 340, 394, 407
Mph1103I ATGCAT 1 cut(s) 222
MreI CGCCGGCG 1 cut(s) 68
MroNI GCCGGC 1 cut(s) 68
MseI TTAA 1 cut(s) 518
MslI CAYNNNNRTG 1 cut(s) 452
MspA1I CMGCKG 3 cut(s) 209, 234, 320
MspI CCGG 5 cut(s) 42, 69, 225, 262, 372
MspR9I CCNGG 3 cut(s) 226, 263, 373
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 1 cut(s) 317
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 3 cut(s) 226, 263, 373
NcoI CCATGG 2 cut(s) 122, 161
NdeII GATC 2 cut(s) 174, 471
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 7 cut(s) 97, 126, 165, 181, 186, 220, 457
NlaIV GGNNCC 2 cut(s) 128, 214
NmuCI GTSAC 1 cut(s) 350
NsiI ATGCAT 1 cut(s) 222
NspI RCATGY 2 cut(s) 97, 186
PagI TCATGA 1 cut(s) 177
PciI ACATGT 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 5 cut(s) 257, 260, 268, 290, 322
PleI GAGTC 2 cut(s) 35, 320
PpsI GAGTC 2 cut(s) 35, 320
PscI ACATGT 1 cut(s) 182
PshAI GACNNNNGTC 1 cut(s) 234
PshBI ATTAAT 1 cut(s) 518
PspEI GGTNACC 1 cut(s) 350
PspN4I GGNNCC 2 cut(s) 128, 214
PspPI GGNCC 2 cut(s) 204, 213
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 452
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 1 cut(s) 518
SatI GCNGC 5 cut(s) 256, 259, 267, 289, 321
Sau3AI GATC 2 cut(s) 174, 471
Sau96I GGNCC 2 cut(s) 204, 213
SchI GAGTC 2 cut(s) 36, 320
ScrFI CCNGG 3 cut(s) 226, 263, 373
SetI ASST 2 cut(s) 322, 346
SfaNI GCATC 2 cut(s) 10, 185
SfcI CTRYAG 1 cut(s) 190
Sfr303I CCGCGG 1 cut(s) 210
SgrAI CRCCGGYG 1 cut(s) 68
SgrBI CCGCGG 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 452
SsiI CCGC 6 cut(s) 72, 207, 209, 234, 288, 323
SspMI CTAG 1 cut(s) 24
StyD4I CCNGG 3 cut(s) 224, 261, 371
StyI CCWWGG 3 cut(s) 122, 161, 246
TaaI ACNGT 1 cut(s) 301
TaqII GACCGA 1 cut(s) 369
TauI GCSGC 1 cut(s) 291
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 1 cut(s) 518
Tru9I TTAA 1 cut(s) 518
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 4 cut(s) 255, 258, 266, 320
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 2 cut(s) 303, 326
TspGWI ACGGA 2 cut(s) 438, 509
VspI ATTAAT 1 cut(s) 518
XceI RCATGY 2 cut(s) 97, 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 168
XspI CTAG 1 cut(s) 24
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.