MD07G1091400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010051
Physical Location & Seq
Forward (+)
9617761 .. 9619861
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1091400.v1.1.491

Sequence Viewer

Length: 693 bp
ATGGTGAAGAAGCAGCTGCTTTGTTGCTGGGGGTGGCTCATAATCTTCAATCATCTGCTACTAGCAGCTCTTGTTTGCTCCAAAAGCCATGGAAACCCTGCAAATGATCTTGTTGACATTATCAACAACAATCGAACAGCTCACAAACTTCCAAAACTAAACGACAGTTCAGGACTCGGGTGCATTGCCTTGCAATATGCTGAGTTATGCAAGGGAAACTGCACTAGCAACAATACCGTGAACTGCAAACCGTCTGAAGATGACTTCACCGAAGTTTTTGCCCCCAACTGTGGTGTGGAACTACCCACTTTCGACACCATAACTGCCCACATTGTGGGTTGTCAATCCAAGTATCTCGAGCCCTCACTGGCGTTTGAACATGTTCTTGTTCGAGACAGCAAGGCTTTATCAATTCTGAGAAATAAATCACATACTGAGGTGGGAGTAGGCATGGTTCGGGCTCACAAAGGGCCATTTTTTTGGTGTGTTCTCTTCAGCAGTGGGAAGACAAACTCCACATTTGTACTGGAAAATCGCGGTGAAGGGATCCATCAAAAGAAAGGTTGCTACAGTGGAAGCAGCATACCATGCAGTGCAGGTCAAAGGAATGATTCCTCGCTGTTGAACAATGTTACTATCATCGCTTTTCTATGTGCTGTTTATCTTTTCCAACAGTTGCACTTCAATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

231

Amino Acids

25.3

Weight (kDa)

8.21

Isoelectric Point (pI)

31.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 587
AccB7I CCANNNNNTGG 1 cut(s) 334
AccII CGCG 1 cut(s) 537
AciI CCGC 1 cut(s) 537
AclWI GGATC 2 cut(s) 541, 554
AcuI CTGAAG 2 cut(s) 276, 478
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 2 cut(s) 290, 334
AflIII ACRYGT 1 cut(s) 379
AgsI TTSAA 4 cut(s) 49, 377, 625, 685
AluBI AGCT 3 cut(s) 16, 68, 140
AluI AGCT 3 cut(s) 16, 68, 140
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 2 cut(s) 541, 554
Ama87I CYCGRG 2 cut(s) 176, 356
AoxI GGCC 1 cut(s) 470
ApeKI GCWGC 4 cut(s) 13, 16, 65, 579
Asp700I GAANNNNTTC 1 cut(s) 381
AspS9I GGNCC 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 16, 259, 551
AvaI CYCGRG 2 cut(s) 176, 356
BamHI GGATCC 1 cut(s) 546
BanII GRGCYC 2 cut(s) 363, 463
BbsI GAAGAC 1 cut(s) 512
BbvI GCAGC 4 cut(s) 3, 25, 77, 591
BccI CCATC 1 cut(s) 558
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 2 cut(s) 62, 225
BfmI CTRYAG 1 cut(s) 568
BfuAI ACCTGC 1 cut(s) 587
BisI GCNGC 4 cut(s) 14, 17, 66, 580
BlsI GCNGC 4 cut(s) 15, 18, 67, 581
BmeT110I CYCGRG 2 cut(s) 176, 356
BmgT120I GGNCC 1 cut(s) 470
BmiI GGNNCC 1 cut(s) 548
BpiI GAAGAC 1 cut(s) 512
BsaJI CCNNGG 1 cut(s) 88
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 334
Bse1I ACTGG 2 cut(s) 372, 531
Bse3DI GCAATG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 88
BseLI CCNNNNNNNGG 2 cut(s) 290, 334
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 3 cut(s) 192, 407, 426
BseNI ACTGG 2 cut(s) 372, 531
BseXI GCAGC 4 cut(s) 3, 25, 77, 591
BseYI CCCAGC 1 cut(s) 27
BsgI GTGCAG 2 cut(s) 205, 615
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 1 cut(s) 472
BsiHKCI CYCGRG 2 cut(s) 176, 356
BslI CCNNNNNNNGG 2 cut(s) 290, 334
BsmAI GTCTC 1 cut(s) 387
BsnI GGCC 1 cut(s) 472
BsoBI CYCGRG 2 cut(s) 176, 356
Bsp1286I GDGCHC 2 cut(s) 363, 463
Bsp143I GATC 2 cut(s) 106, 546
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 1 cut(s) 537
BspANI GGCC 1 cut(s) 472
BspCNI CTCAG 3 cut(s) 193, 408, 427
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 1 cut(s) 548
BspMI ACCTGC 1 cut(s) 587
BspPI GGATC 2 cut(s) 541, 554
BsrDI GCAATG 1 cut(s) 183
BsrI ACTGG 2 cut(s) 372, 531
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 2 cut(s) 106, 546
BssT1I CCWWGG 1 cut(s) 88
Bst4CI ACNGT 6 cut(s) 167, 238, 252, 290, 572, 675
Bst6I CTCTTC 1 cut(s) 497
BstAPI GCANNNNNTGC 1 cut(s) 588
BstDEI CTNAG 3 cut(s) 201, 416, 435
BstDSI CCRYGG 1 cut(s) 88
BstFNI CGCG 1 cut(s) 537
BstKTI GATC 2 cut(s) 109, 549
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 2 cut(s) 106, 546
BstMWI GCNNNNNNNGC 2 cut(s) 84, 588
BstNSI RCATGY 1 cut(s) 383
BstSFI CTRYAG 1 cut(s) 568
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 4 cut(s) 3, 25, 77, 591
BstV2I GAAGAC 1 cut(s) 512
BstX2I RGATCY 1 cut(s) 546
BstXI CCANNNNNNTGG 1 cut(s) 480
BstYI RGATCY 1 cut(s) 546
BsuRI GGCC 1 cut(s) 472
BtgI CCRYGG 1 cut(s) 88
BtgZI GCGATG 1 cut(s) 625
BtsI GCAGTG 2 cut(s) 505, 598
BtsIMutI CAGTG 4 cut(s) 365, 505, 577, 598
BveI ACCTGC 1 cut(s) 587
Cfr13I GGNCC 1 cut(s) 470
Csp6I GTAC 1 cut(s) 524
CviAII CATG 4 cut(s) 89, 380, 451, 588
CviJI RGCY 9 cut(s) 16, 37, 68, 87, 140, 361, 404, 461, 472
CviKI_1 RGCY 9 cut(s) 16, 37, 68, 87, 140, 361, 404, 461, 472
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 3 cut(s) 201, 416, 435
DpnI GATC 2 cut(s) 108, 548
DpnII GATC 2 cut(s) 106, 546
DraIII CACNNNGTG 1 cut(s) 334
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 88
Eco24I GRGCYC 2 cut(s) 363, 463
Eco57I CTGAAG 2 cut(s) 276, 478
Eco88I CYCGRG 2 cut(s) 176, 356
EcoT14I CCWWGG 1 cut(s) 88
EcoT38I GRGCYC 2 cut(s) 363, 463
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 4 cut(s) 92, 383, 454, 591
FatI CATG 4 cut(s) 88, 379, 450, 587
Fnu4HI GCNGC 4 cut(s) 14, 17, 66, 580
FriOI GRGCYC 2 cut(s) 363, 463
Fsp4HI GCNGC 4 cut(s) 14, 17, 66, 580
FspBI CTAG 2 cut(s) 62, 225
GluI GCNGC 4 cut(s) 14, 17, 66, 580
GsaI CCCAGC 1 cut(s) 31
HaeIII GGCC 1 cut(s) 472
Hin1II CATG 4 cut(s) 92, 383, 454, 591
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 174, 611
HphI GGTGA 3 cut(s) 16, 259, 551
Hpy166II GTNNAC 2 cut(s) 115, 241
Hpy188I TCNGA 2 cut(s) 256, 417
Hpy188III TCNNGA 3 cut(s) 171, 356, 392
Hpy8I GTNNAC 2 cut(s) 115, 241
HpyAV CCTTC 1 cut(s) 536
HpyCH4III ACNGT 6 cut(s) 167, 238, 252, 290, 572, 675
HpyCH4V TGCA 9 cut(s) 101, 183, 193, 210, 222, 246, 591, 596, 679
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 588
HpyF3I CTNAG 3 cut(s) 201, 416, 435
Hsp92II CATG 4 cut(s) 92, 383, 454, 591
Kzo9I GATC 2 cut(s) 106, 546
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 6 cut(s) 13, 111, 156, 353, 512, 582
Lsp1109I GCAGC 4 cut(s) 3, 25, 77, 591
MaeI CTAG 2 cut(s) 62, 225
MaeIII GTNAC 1 cut(s) 631
MalI GATC 2 cut(s) 108, 548
MboI GATC 2 cut(s) 106, 546
MboII GAAGA 5 cut(s) 19, 37, 269, 484, 517
MflI RGATCY 1 cut(s) 546
MhlI GDGCHC 2 cut(s) 363, 463
MluCI AATT 2 cut(s) 411, 685
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 3 cut(s) 373, 430, 625
MroXI GAANNNNTTC 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 16
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 2 cut(s) 84, 588
NcoI CCATGG 1 cut(s) 88
NdeII GATC 2 cut(s) 106, 546
NlaIII CATG 4 cut(s) 92, 383, 454, 591
NlaIV GGNNCC 1 cut(s) 548
NspI RCATGY 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 356
PciI ACATGT 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 381
PfeI GAWTC 1 cut(s) 611
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 4 cut(s) 15, 18, 67, 581
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
PscI ACATGT 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 548
PspPI GGNCC 1 cut(s) 470
PsrI GAACNNNNNNTAC 2 cut(s) 438, 470
PsuI RGATCY 1 cut(s) 546
PvuII CAGCTG 1 cut(s) 16
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
SatI GCNGC 4 cut(s) 14, 17, 66, 580
Sau3AI GATC 2 cut(s) 106, 546
Sau96I GGNCC 1 cut(s) 470
SchI GAGTC 1 cut(s) 168
SduI GDGCHC 2 cut(s) 363, 463
SetI ASST 6 cut(s) 18, 70, 142, 441, 565, 601
SfcI CTRYAG 1 cut(s) 568
Sfr274I CTCGAG 1 cut(s) 356
SlaI CTCGAG 1 cut(s) 356
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 2 cut(s) 411, 685
SsiI CCGC 1 cut(s) 537
SspMI CTAG 2 cut(s) 62, 225
StyI CCWWGG 1 cut(s) 88
TaaI ACNGT 6 cut(s) 167, 238, 252, 290, 572, 675
TaqI TCGA 4 cut(s) 133, 312, 357, 391
TasI AATT 2 cut(s) 411, 685
TatI WGTACW 1 cut(s) 523
TfiI GAWTC 1 cut(s) 611
TscAI CASTG 4 cut(s) 372, 505, 577, 598
TseI GCWGC 4 cut(s) 13, 16, 65, 579
TspRI CASTG 4 cut(s) 372, 505, 577, 598
Van91I CCANNNNNTGG 1 cut(s) 334
XceI RCATGY 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 2 cut(s) 292, 523
XhoI CTCGAG 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 381
XspI CTAG 2 cut(s) 62, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.