MD07G1109600.v1.1

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009898
Physical Location & Seq
Forward (+)
12609273 .. 12610232
960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1109600.v1.1.491

Sequence Viewer

Length: 681 bp
ATGATTCTCTCTCATTCCTATTTGTATATTACTAACAACTCATTATACTTTCAGCTAGCTGCCTCCCTATATATGACATATTACATGCTCAAGGGTCCTCAGCACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAACTACACAGAGAAGACTTCAAACATGGCCATTGCAACAGCTCTGCTGGTCCTCCTGCTGGCAGCCCCAACAGTGTATGGAGTGCAGCACATTGTCGGTGACGCCTCTGGTTGGAGCACATCGGTAGATTATGACTCTTGGGCTGCAAGTAACGCCTTCACTGTTGGTGACACTCTCGTGTTCAACTATGGTAGTTCCCACAAAGTGGATATTGTAAACCAAGCTGACTTCGCCAGTTGCAGTTCAAGCAATTCCCTAAAAACCTATGATGATGGCGCCACAACAATCACCCTCTCCCAACCTGGTCCAGCCTACTTCATATGCCCTTCTGCAGGTCACTGTGGTAATGGCATGAAGCTTTCGGTCAACGTTGTAGCAGCTGGCACCCCAAGTGGTACTCCGTCAACCACCCCATCTACACCCACCACTCCTGGAAGCATTGTTTCACCACCGCCTCCTAAGGGAGCAGCAAATACTCTTGACATGAATATGCTTGGGTTTCCACTTGTGTTGGCAACCTTGGTTGCATTCATGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

227

Amino Acids

23.62

Weight (kDa)

5.54

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 85 - 165 7.5e-25 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 464
AccB1I GGYRCC 2 cut(s) 416, 524
AccB7I CCANNNNNTGG 1 cut(s) 346
AciI CCGC 1 cut(s) 593
AclI AACGTT 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 168
AcyI GRCGYC 2 cut(s) 243, 417
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 1 cut(s) 538
AfiI CCNNNNNNNGG 5 cut(s) 199, 252, 346, 473, 602
AgsI TTSAA 3 cut(s) 162, 325, 387
AjnI CCWGG 2 cut(s) 442, 571
AleI CACNNNNGTG 1 cut(s) 317
AluBI AGCT 6 cut(s) 55, 59, 182, 365, 499, 521
AluI AGCT 6 cut(s) 55, 59, 182, 365, 499, 521
Alw21I GWGCWC 1 cut(s) 260
AoxI GGCC 1 cut(s) 168
ApeKI GCWGC 6 cut(s) 59, 203, 226, 284, 518, 608
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 3 cut(s) 95, 190, 446
AsuHPI GGTGA 4 cut(s) 251, 320, 421, 579
AsuNHI GCTAGC 1 cut(s) 55
AvaII GGWCC 3 cut(s) 95, 190, 446
AxyI CCTNAGG 1 cut(s) 600
BalI TGGCCA 1 cut(s) 170
BanI GGYRCC 2 cut(s) 416, 524
BauI CACGAG 1 cut(s) 317
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 260
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 6 cut(s) 46, 215, 238, 271, 530, 620
BccI CCATC 2 cut(s) 407, 562
BciT130I CCWGG 2 cut(s) 444, 573
BfaI CTAG 2 cut(s) 56, 679
BfmI CTRYAG 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 420
BfuAI ACCTGC 1 cut(s) 464
BisI GCNGC 6 cut(s) 60, 204, 227, 285, 519, 609
BlsI GCNGC 6 cut(s) 61, 205, 228, 286, 520, 610
Bme1390I CCNGG 2 cut(s) 444, 573
Bme18I GGWCC 3 cut(s) 95, 190, 446
BmgT120I GGNCC 3 cut(s) 95, 190, 446
BmiI GGNNCC 3 cut(s) 96, 418, 526
BmrFI CCNGG 2 cut(s) 444, 573
BmtI GCTAGC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 161
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 74
BsaHI GRCGYC 2 cut(s) 243, 417
BsaJI CCNNGG 1 cut(s) 660
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 252, 346, 473, 602
Bse1I ACTGG 1 cut(s) 375
Bse21I CCTNAGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 2 cut(s) 444, 573
BseDI CCNNGG 1 cut(s) 660
BseLI CCNNNNNNNGG 5 cut(s) 199, 252, 346, 473, 602
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 6 cut(s) 46, 215, 238, 271, 530, 620
BsgI GTGCAG 1 cut(s) 245
BshFI GGCC 1 cut(s) 170
BshNI GGYRCC 2 cut(s) 416, 524
BsiHKAI GWGCWC 1 cut(s) 260
BslI CCNNNNNNNGG 5 cut(s) 199, 252, 346, 473, 602
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 260
BspACI CCGC 1 cut(s) 593
BspANI GGCC 1 cut(s) 170
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 3 cut(s) 96, 418, 526
BspMAI CTGCAG 1 cut(s) 475
BspMI ACCTGC 1 cut(s) 464
BspOI GCTAGC 1 cut(s) 59
BspT107I GGYRCC 2 cut(s) 416, 524
BsrDI GCAATG 1 cut(s) 171
BsrI ACTGG 1 cut(s) 375
BssECI CCNNGG 1 cut(s) 660
BssNI GRCGYC 2 cut(s) 243, 417
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 317
Bst2UI CCWGG 2 cut(s) 444, 573
Bst4CI ACNGT 3 cut(s) 214, 304, 482
BstACI GRCGYC 2 cut(s) 243, 417
BstC8I GCNNGC 3 cut(s) 57, 201, 523
BstDEI CTNAG 2 cut(s) 99, 600
BstENI CCTNNNNNAGG 1 cut(s) 471
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstMWI GCNNNNNNNGC 3 cut(s) 293, 371, 387
BstNI CCWGG 2 cut(s) 444, 573
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 2 cut(s) 442, 571
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 6 cut(s) 46, 215, 238, 271, 530, 620
BstV2I GAAGAC 1 cut(s) 161
Bsu36I CCTNAGG 1 cut(s) 600
BsuRI GGCC 1 cut(s) 170
BtsIMutI CAGTG 3 cut(s) 219, 300, 478
BveI ACCTGC 1 cut(s) 464
Cac8I GCNNGC 3 cut(s) 57, 201, 523
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 3 cut(s) 95, 190, 446
CseI GACGC 1 cut(s) 251
CsiI ACCWGGT 1 cut(s) 442
Csp6I GTAC 1 cut(s) 537
CspCI CAANNNNNGTGG 2 cut(s) 633, 668
CviAII CATG 5 cut(s) 85, 166, 493, 625, 673
CviQI GTAC 1 cut(s) 537
DdeI CTNAG 2 cut(s) 99, 600
DinI GGCGCC 1 cut(s) 418
DraIII CACNNNGTG 1 cut(s) 346
EaeI YGGCCR 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 660
Eco47I GGWCC 3 cut(s) 95, 190, 446
Eco81I CCTNAGG 1 cut(s) 600
EcoNI CCTNNNNNAGG 1 cut(s) 471
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 2 cut(s) 442, 571
EcoT14I CCWWGG 1 cut(s) 660
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 5 cut(s) 88, 169, 496, 628, 676
FatI CATG 5 cut(s) 84, 165, 492, 624, 672
FauNDI CATATG 1 cut(s) 461
Fnu4HI GCNGC 6 cut(s) 60, 204, 227, 285, 519, 609
Fsp4HI GCNGC 6 cut(s) 60, 204, 227, 285, 519, 609
FspBI CTAG 2 cut(s) 56, 679
GlaI GCGC 1 cut(s) 418
GluI GCNGC 6 cut(s) 60, 204, 227, 285, 519, 609
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 1 cut(s) 170
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 419
Hin1I GRCGYC 2 cut(s) 243, 417
Hin1II CATG 5 cut(s) 88, 169, 496, 628, 676
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HincII GTYRAC 2 cut(s) 508, 546
HindII GTYRAC 2 cut(s) 508, 546
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 2 cut(s) 4, 275
HphI GGTGA 4 cut(s) 251, 320, 421, 579
Hpy166II GTNNAC 3 cut(s) 358, 508, 546
Hpy188III TCNNGA 1 cut(s) 620
Hpy8I GTNNAC 3 cut(s) 358, 508, 546
HpyAV CCTTC 2 cut(s) 307, 477
HpyCH4III ACNGT 3 cut(s) 214, 304, 482
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 6 cut(s) 176, 226, 287, 381, 473, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 371, 387
HpyF3I CTNAG 2 cut(s) 99, 600
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 2 cut(s) 243, 417
Hsp92II CATG 5 cut(s) 88, 169, 496, 628, 676
HspAI GCGC 1 cut(s) 417
KasI GGCGCC 1 cut(s) 416
LmnI GCTCC 2 cut(s) 255, 605
Lsp1109I GCAGC 6 cut(s) 46, 215, 238, 271, 530, 620
MabI ACCWGGT 1 cut(s) 442
MaeI CTAG 2 cut(s) 56, 679
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 4 cut(s) 239, 290, 308, 476
MboII GAAGA 1 cut(s) 166
MhlI GDGCHC 1 cut(s) 260
MlsI TGGCCA 1 cut(s) 170
MluCI AATT 1 cut(s) 391
MluNI TGGCCA 1 cut(s) 170
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 6 cut(s) 73, 108, 203, 256, 443, 606
Mox20I TGGCCA 1 cut(s) 170
MscI TGGCCA 1 cut(s) 170
MslI CAYNNNNRTG 2 cut(s) 317, 629
Msp20I TGGCCA 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 521
MspR9I CCNGG 2 cut(s) 444, 573
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 2 cut(s) 444, 573
MwoI GCNNNNNNNGC 3 cut(s) 293, 371, 387
NarI GGCGCC 1 cut(s) 417
NdeI CATATG 1 cut(s) 461
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 5 cut(s) 88, 169, 496, 628, 676
NlaIV GGNNCC 3 cut(s) 96, 418, 526
NmuCI GTSAC 3 cut(s) 239, 308, 476
NspI RCATGY 1 cut(s) 88
OliI CACNNNNGTG 1 cut(s) 317
PctI GAATGC 1 cut(s) 668
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 6 cut(s) 61, 205, 228, 286, 520, 610
PleI GAGTC 1 cut(s) 269
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 1 cut(s) 269
PpuMI RGGWCCY 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 442, 571
PspGI CCWGG 2 cut(s) 442, 571
PspN4I GGNNCC 3 cut(s) 96, 418, 526
PspPI GGNCC 3 cut(s) 95, 190, 446
PspPPI RGGWCCY 1 cut(s) 95
PstI CTGCAG 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 1 cut(s) 538
RsaNI GTAC 1 cut(s) 537
RseI CAYNNNNRTG 2 cut(s) 317, 629
SatI GCNGC 6 cut(s) 60, 204, 227, 285, 519, 609
Sau96I GGNCC 3 cut(s) 95, 190, 446
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 2 cut(s) 444, 573
SduI GDGCHC 1 cut(s) 260
SexAI ACCWGGT 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 471
SfoI GGCGCC 1 cut(s) 418
SinI GGWCC 3 cut(s) 95, 190, 446
SmiMI CAYNNNNRTG 2 cut(s) 317, 629
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 1 cut(s) 391
SsiI CCGC 1 cut(s) 593
SspDI GGCGCC 1 cut(s) 416
SspMI CTAG 2 cut(s) 56, 679
StyD4I CCNGG 2 cut(s) 442, 571
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 3 cut(s) 214, 304, 482
TaiI ACGT 1 cut(s) 513
TaqII GACCGA 1 cut(s) 493
TasI AATT 1 cut(s) 391
TfiI GAWTC 1 cut(s) 4
TscAI CASTG 3 cut(s) 219, 307, 485
TseFI GTSAC 3 cut(s) 239, 308, 476
TseI GCWGC 6 cut(s) 59, 203, 226, 284, 518, 608
Tsp45I GTSAC 3 cut(s) 239, 308, 476
TspDTI ATGAA 4 cut(s) 448, 509, 641, 661
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 3 cut(s) 219, 307, 485
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 3 cut(s) 95, 190, 446
XagI CCTNNNNNAGG 1 cut(s) 471
XceI RCATGY 1 cut(s) 88
XspI CTAG 2 cut(s) 56, 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.