MD07G1135900.v1.1

Cell division control protein 14, SIN component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014509
Physical Location & Seq
Reverse (-)
19820119 .. 19822107
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1135900.v1.1.491

Sequence Viewer

Length: 900 bp
ATGTACTTGAAGAAGCCGCTATGGAGCGAGGGTATAATACCGAAGGGCTCGTCGGGATCTGAACAAGAACAGCCATCCACCGCCGTCGGCGATCTGGTCAACTCGTTGACCCAGCAAAGAGTGTACCGCGAGGTCACGCTCGCGCTCCGCAGCGGGCTGCGCGACGTTCGCGCCGAGTTCTCGTTCCTCCGTGTCCGCGGCCTCCGCGGCCTCCTTAAGTTCCTCCGATCGGTCGCCGAGACCGACTCCACTATCAACCTCTTCTCTCAGACCCAATCGCTCCCCGAGCTTCAAGTGGTGCCGGTGCTGTTTCAGCACTCGCTGAAAGATTCGGGCGATGAGATCGTTCGGAATTTGGATCACATTTTCGGCGTCGAGCCGCTGAAAATCACGAGCCCTTCAACCGATTCCGAGGTCTCGCTCGCGCTTAGGGTTTTGGAGGGTTGCTGCCTGCTTCATCCGGACAGCGCGGTCTTGGCTCACCAGCACAAAGCCATTCAGGTGTTGATGAATATATTACCGACTCGCGGCGTGATTGAGCAAGGAGCTTGTTTGGATGCTTTGATTTCTATTATGTTGGATTCATCAGCTAATCAAATGGATTTTGAGGCTTTTCATGGTATTGAGGAAGTTGCGGAGCTCATAAGAGATAAGCAAGTTGATGAGAATCTCAGGTTGAAATGTGGAGAGTTCTTGCTGCTGCTCATTGGTCACGTAAATGGGAGGGATGGGCCACCCTTGGCAACTGTACATGAGGACATAAGGCGGCTTTTGGGTGAGAAATCTGCTTCCTTGATATGGGCAGCCAGTCAGTTTGGTTCGACTCTTGATCCGGAGCAGAGACTAACTGCACTTCACATCCAAGCTCGTCGTGTGATAGAGTCCTTAGACTTGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CDC14

Protein Analysis

300

Amino Acids

33.16

Weight (kDa)

5.51

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC14 PF08045 131 - 233 1.1e-12 Cell division control protein 14, SIN component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27750
fragaria_vesca FvH4_7g14460
malus_domestica MD01G1064000.v1.1 MD01G1064500.v1.1 MD07G1135900.v1.1
prunus_persica Prupe.2G171600_v2.0.a1
pyrus_communis pycom07g13330
rosa_chinensis RchiOBHm_Chr1g0355871
rosa_laevigata RLG00000028120
rosa_multiflora Rmu_sc0002284.1_g000020 Rmu_sc0004705.1_g000020
rosa_roxburghii Rroxscaffold_4G00299960
rosa_rugosa Rorug01G0248100
rosa_samantha Rh1AG261300 Rh1CG244400 Rh1DG258000
rosa_wichuraiana Rw1G023070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
AccB1I GGYRCC 1 cut(s) 298
AccII CGCG 9 cut(s) 129, 143, 162, 171, 198, 207, 425, 470, 528
AccIII TCCGGA 2 cut(s) 460, 832
AclWI GGATC 3 cut(s) 64, 366, 824
AcsI RAATTY 1 cut(s) 352
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 4 cut(s) 5, 125, 750, 896
AfiI CCNNNNNNNGG 3 cut(s) 229, 527, 798
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 4 cut(s) 10, 293, 402, 679
AluBI AGCT 5 cut(s) 289, 548, 590, 640, 866
AluI AGCT 5 cut(s) 289, 548, 590, 640, 866
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 3 cut(s) 233, 421, 835
AlwI GGATC 3 cut(s) 64, 366, 824
Ama87I CYCGRG 1 cut(s) 284
Aor13HI TCCGGA 2 cut(s) 460, 832
AoxI GGCC 3 cut(s) 199, 208, 731
ApeKI GCWGC 6 cut(s) 150, 157, 447, 697, 700, 803
ApoI RAATTY 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 35, 67
AspLEI GCGC 5 cut(s) 145, 162, 173, 427, 470
AspS9I GGNCC 1 cut(s) 731
AsuHPI GGTGA 2 cut(s) 473, 788
AvaI CYCGRG 1 cut(s) 284
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 3 cut(s) 50, 398, 642
BauI CACGAG 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 6 cut(s) 144, 162, 434, 684, 687, 815
BccI CCATC 2 cut(s) 82, 722
BceAI ACGGC 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 233, 421, 835
BfrI CTTAAG 1 cut(s) 215
BglI GCCNNNNNGGC 1 cut(s) 207
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 731
BmiI GGNNCC 1 cut(s) 300
BmsI GCATC 1 cut(s) 547
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu10I CCTNAGC 1 cut(s) 428
BsaAI YACGTR 1 cut(s) 715
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 1 cut(s) 372
BsaI GGTCTC 2 cut(s) 233, 421
BsaJI CCNNGG 4 cut(s) 196, 205, 411, 738
BsaWI WCCGGW 2 cut(s) 460, 832
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 527, 798
Bse118I RCCGGY 1 cut(s) 301
Bse1I ACTGG 1 cut(s) 807
Bse8I GATNNNNATC 1 cut(s) 666
BseAI TCCGGA 2 cut(s) 460, 832
BseDI CCNNGG 4 cut(s) 196, 205, 411, 738
BseGI GGATG 5 cut(s) 74, 457, 562, 733, 858
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 3 cut(s) 229, 527, 798
BseMII CTCAG 2 cut(s) 281, 685
BseNI ACTGG 1 cut(s) 807
BseXI GCAGC 6 cut(s) 144, 162, 434, 684, 687, 815
BseYI CCCAGC 1 cut(s) 111
BsgI GTGCAG 1 cut(s) 834
Bsh1236I CGCG 9 cut(s) 129, 143, 162, 171, 198, 207, 425, 470, 528
Bsh1285I CGRYCG 2 cut(s) 230, 234
BshFI GGCC 3 cut(s) 201, 210, 733
BshNI GGYRCC 1 cut(s) 298
BsiEI CGRYCG 2 cut(s) 230, 234
BsiHKAI GWGCWC 1 cut(s) 642
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 3 cut(s) 302, 461, 833
BslI CCNNNNNNNGG 3 cut(s) 229, 527, 798
BsmAI GTCTC 3 cut(s) 233, 421, 835
BsnI GGCC 3 cut(s) 201, 210, 733
Bso31I GGTCTC 2 cut(s) 233, 421
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 3 cut(s) 50, 398, 642
Bsp13I TCCGGA 2 cut(s) 460, 832
Bsp1407I TGTACA 1 cut(s) 748
Bsp143I GATC 6 cut(s) 56, 91, 227, 342, 358, 829
BspANI GGCC 3 cut(s) 201, 210, 733
BspCNI CTCAG 2 cut(s) 280, 684
BspEI TCCGGA 2 cut(s) 460, 832
BspFNI CGCG 9 cut(s) 129, 143, 162, 171, 198, 207, 425, 470, 528
BspLI GGNNCC 1 cut(s) 300
BspPI GGATC 3 cut(s) 64, 366, 824
BspT107I GGYRCC 1 cut(s) 298
BspTI CTTAAG 1 cut(s) 215
BspTNI GGTCTC 2 cut(s) 233, 421
BsrFI RCCGGY 1 cut(s) 301
BsrGI TGTACA 1 cut(s) 748
BsrI ACTGG 1 cut(s) 807
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 4 cut(s) 196, 205, 411, 738
BssMI GATC 6 cut(s) 56, 91, 227, 342, 358, 829
BssNI GRCGYC 1 cut(s) 372
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 738
Bst2BI CACGAG 1 cut(s) 391
Bst4CI ACNGT 1 cut(s) 748
Bst6I CTCTTC 1 cut(s) 266
BstACI GRCGYC 1 cut(s) 372
BstAFI CTTAAG 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 748
BstBAI YACGTR 1 cut(s) 715
BstC8I GCNNGC 4 cut(s) 141, 155, 423, 452
BstDEI CTNAG 4 cut(s) 267, 428, 671, 886
BstDSI CCRYGG 2 cut(s) 196, 205
BstF5I GGATG 5 cut(s) 74, 457, 562, 733, 858
BstFNI CGCG 9 cut(s) 129, 143, 162, 171, 198, 207, 425, 470, 528
BstHHI GCGC 5 cut(s) 145, 162, 173, 427, 470
BstKTI GATC 6 cut(s) 59, 94, 230, 345, 361, 832
BstMAI GTCTC 3 cut(s) 233, 421, 835
BstMBI GATC 6 cut(s) 56, 91, 227, 342, 358, 829
BstMCI CGRYCG 2 cut(s) 230, 234
BstMWI GCNNNNNNNGC 7 cut(s) 159, 168, 204, 207, 286, 313, 476
BstUI CGCG 9 cut(s) 129, 143, 162, 171, 198, 207, 425, 470, 528
BstV1I GCAGC 6 cut(s) 144, 162, 434, 684, 687, 815
BstX2I RGATCY 1 cut(s) 56
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 3 cut(s) 201, 210, 733
BtgI CCRYGG 2 cut(s) 196, 205
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 5 cut(s) 74, 457, 562, 733, 858
Cac8I GCNNGC 4 cut(s) 141, 155, 423, 452
CfoI GCGC 5 cut(s) 145, 162, 173, 427, 470
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 731
Cfr42I CCGCGG 2 cut(s) 199, 208
CseI GACGC 1 cut(s) 361
Csp6I GTAC 4 cut(s) 4, 124, 749, 895
CviAII CATG 2 cut(s) 617, 752
CviQI GTAC 4 cut(s) 4, 124, 749, 895
DdeI CTNAG 4 cut(s) 267, 428, 671, 886
DpnI GATC 6 cut(s) 58, 93, 229, 344, 360, 831
DpnII GATC 6 cut(s) 56, 91, 227, 342, 358, 829
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 1 cut(s) 738
Eco24I GRGCYC 3 cut(s) 50, 398, 642
Eco31I GGTCTC 2 cut(s) 233, 421
Eco53kI GAGCTC 1 cut(s) 640
Eco88I CYCGRG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 738
EcoT38I GRGCYC 3 cut(s) 50, 398, 642
ErhI CCWWGG 1 cut(s) 738
FaeI CATG 2 cut(s) 620, 755
FaiI YATR 9 cut(s) 22, 35, 515, 575, 618, 644, 753, 761, 799
FatI CATG 2 cut(s) 616, 751
FauI CCCGC 1 cut(s) 146
FokI GGATG 5 cut(s) 61, 444, 569, 740, 845
FriOI GRGCYC 3 cut(s) 50, 398, 642
GlaI GCGC 5 cut(s) 144, 161, 172, 426, 469
GsaI CCCAGC 1 cut(s) 115
HaeIII GGCC 3 cut(s) 201, 210, 733
HapII CCGG 3 cut(s) 302, 461, 833
HgaI GACGC 1 cut(s) 361
HhaI GCGC 5 cut(s) 145, 162, 173, 427, 470
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 2 cut(s) 620, 755
Hin6I GCGC 5 cut(s) 143, 160, 171, 425, 468
HinP1I GCGC 5 cut(s) 143, 160, 171, 425, 468
HincII GTYRAC 2 cut(s) 100, 108
HindII GTYRAC 2 cut(s) 100, 108
HinfI GANTC 8 cut(s) 245, 329, 407, 523, 581, 667, 823, 881
HpaII CCGG 3 cut(s) 302, 461, 833
HphI GGTGA 2 cut(s) 473, 788
Hpy166II GTNNAC 3 cut(s) 100, 108, 124
Hpy188I TCNGA 5 cut(s) 61, 227, 270, 351, 412
Hpy188III TCNNGA 5 cut(s) 54, 391, 461, 827, 833
Hpy8I GTNNAC 3 cut(s) 100, 108, 124
Hpy99I CGWCG 5 cut(s) 55, 89, 167, 377, 873
HpyAV CCTTC 2 cut(s) 37, 408
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4IV ACGT 2 cut(s) 165, 714
HpyCH4V TGCA 1 cut(s) 851
HpyF10VI GCNNNNNNNGC 7 cut(s) 159, 168, 204, 207, 286, 313, 476
HpyF3I CTNAG 4 cut(s) 267, 428, 671, 886
HpySE526I ACGT 2 cut(s) 165, 714
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 2 cut(s) 620, 755
HspAI GCGC 5 cut(s) 143, 160, 171, 425, 468
Kpn2I TCCGGA 2 cut(s) 460, 832
KspI CCGCGG 2 cut(s) 199, 208
Kzo9I GATC 6 cut(s) 56, 91, 227, 342, 358, 829
LmnI GCTCC 6 cut(s) 24, 150, 285, 545, 637, 835
Lsp1109I GCAGC 6 cut(s) 144, 162, 434, 684, 687, 815
LweI GCATC 1 cut(s) 547
MaeII ACGT 2 cut(s) 165, 714
MaeIII GTNAC 2 cut(s) 133, 710
MalI GATC 6 cut(s) 58, 93, 229, 344, 360, 831
MboI GATC 6 cut(s) 56, 91, 227, 342, 358, 829
MboII GAAGA 2 cut(s) 22, 253
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 3 cut(s) 50, 398, 642
MluCI AATT 1 cut(s) 352
MlyI GAGTC 4 cut(s) 239, 517, 817, 890
MmeI TCCRAC 1 cut(s) 558
MroI TCCGGA 2 cut(s) 460, 832
MseI TTAA 1 cut(s) 216
MslI CAYNNNNRTG 2 cut(s) 500, 717
MspA1I CMGCKG 4 cut(s) 153, 198, 207, 382
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 3 cut(s) 302, 461, 833
MvnI CGCG 9 cut(s) 129, 143, 162, 171, 198, 207, 425, 470, 528
MwoI GCNNNNNNNGC 7 cut(s) 159, 168, 204, 207, 286, 313, 476
NdeII GATC 6 cut(s) 56, 91, 227, 342, 358, 829
NlaIII CATG 2 cut(s) 620, 755
NlaIV GGNNCC 1 cut(s) 300
NmeAIII GCCGAG 2 cut(s) 199, 262
NmuCI GTSAC 2 cut(s) 133, 710
PcsI WCGNNNNNNNCGW 2 cut(s) 171, 240
PfeI GAWTC 4 cut(s) 329, 407, 581, 667
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 4 cut(s) 239, 517, 817, 889
PpsI GAGTC 4 cut(s) 239, 517, 817, 889
Ppu21I YACGTR 1 cut(s) 715
Psp124BI GAGCTC 1 cut(s) 642
PspFI CCCAGC 1 cut(s) 111
PspN4I GGNNCC 1 cut(s) 300
PspPI GGNCC 1 cut(s) 731
PsuI RGATCY 1 cut(s) 56
PvuI CGATCG 1 cut(s) 230
RsaI GTAC 4 cut(s) 5, 125, 750, 896
RsaNI GTAC 4 cut(s) 4, 124, 749, 895
RseI CAYNNNNRTG 2 cut(s) 500, 717
SacI GAGCTC 1 cut(s) 642
SacII CCGCGG 2 cut(s) 199, 208
SaqAI TTAA 1 cut(s) 216
Sau3AI GATC 6 cut(s) 56, 91, 227, 342, 358, 829
Sau96I GGNCC 1 cut(s) 731
SchI GAGTC 4 cut(s) 239, 517, 817, 890
SduI GDGCHC 3 cut(s) 50, 398, 642
SfaNI GCATC 1 cut(s) 547
SfiI GGCCNNNNNGGCC 1 cut(s) 207
Sfr303I CCGCGG 2 cut(s) 199, 208
SgrBI CCGCGG 2 cut(s) 199, 208
SmiMI CAYNNNNRTG 2 cut(s) 500, 717
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 1 cut(s) 352
SstI GAGCTC 1 cut(s) 642
StyI CCWWGG 1 cut(s) 738
TaaI ACNGT 1 cut(s) 748
TaiI ACGT 2 cut(s) 168, 717
TaqI TCGA 2 cut(s) 375, 821
TaqII GACCGA 2 cut(s) 220, 257
TasI AATT 1 cut(s) 352
TatI WGTACW 3 cut(s) 3, 748, 894
TauI GCSGC 6 cut(s) 19, 201, 210, 382, 531, 769
TfiI GAWTC 4 cut(s) 329, 407, 581, 667
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TseFI GTSAC 2 cut(s) 133, 710
TseI GCWGC 6 cut(s) 150, 157, 447, 697, 700, 803
Tsp45I GTSAC 2 cut(s) 133, 710
TspDTI ATGAA 4 cut(s) 446, 524, 573, 605
TspGWI ACGGA 1 cut(s) 179
Vha464I CTTAAG 1 cut(s) 215
XapI RAATTY 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.