MD07G1138800.v1.1

Seven transmembrane domain-containing tyrosine-protein kinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013645
Physical Location & Seq
Forward (+)
20158005 .. 20160972
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1138800.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGCAGAAGTCTCAAAAGGTGGTTGGATTTGAAGGTCCATACCGGCTGCTATATTGTTCGAGCAAGGGTGACAAAGCAGGAGTGATGCGCGAGCTAGAGAAAGGGGTGGAGCCGGATTTAGCTGACTATGATAAGAGAACAGCTCTTCACTTGGCATCCTGTGAAGGCTGCACTGAGATTGTTGTTCTTCTTCTTCAGAAAGGTGCTGATGTGAACTCCACAGATCGCTGGGGCCGCACTCCACTATCTGATGCTCGAAGCTTCAGTCACGAGGCGATTTGCAAGATATTGGAGGCTAAGGGTGGAATAGATCCAGTAGGGCTTGATTCCCAGCTTCCATGCTATGAACTTGATCACAGAGAGGTGAACATGGATGAAGCAGCACTTATTGGAGAGGGATCATATGGTGAGATTTATTTAGTGAGGTGGCGCGGTACAGAAGTTGCTGCTAAAACAATCCGCTCGTCCATTGCTTCCAATCCAATAGTGAAGAATATCTTTATGAAGGAACTCGCTTTGTGGCAAAAGTTGCGCCACCCTAATATCGTGCAATTCCTTGGTGTTTTGAAGCACCCTGACCGCTTGATCTTCCTTACCGAGTATCTTTGCAATGGAAGTTTGCATGATATATTGAAGAGAAAAGGAAGACTTGATCTACAAACTGTAGTTTCTTTTGCTCTAGATATCGCCAGAAATGTTTTACAAGATGAAGCCGGGCGCCTCAAGGTCACGGATTTTGGCTTAAGTAAAATTGCACAGGAAAATGATGTATCGGGTTATAAAATGACTGGAAGAACAGGTTCATATCGTTATATGGCACCTGAGGTTTATCGTCGAGAATCATATGGAACAAGTATTGATATCTTCTCGTTCGCTCTCATAGTACATGAGATGTTTCTCGGAGGACCACCTAATCTGGCAGAGGATCCAGAAAAAATTGCAGACAAGCGCGCATATGAGGATTCTCGACCACCTCTCTACTCTTATTTGTACCCCGAACCAATCAGGACGCTTCTGAAAGAATGCTGGCACCAGAATCCAGATTGCCGGCCGACGTTTGAAGAGATTATTGTGAAGCTGGAGGAGATTCAGGATAAGTTAAAGAACAAGAGGAAAATGGGTACGTGTAGCGGTGTTTGTGTCATTTCCTAA

Protein Analysis

384

Amino Acids

43.24

Weight (kDa)

6.48

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 17 - 104 3e-11 Ankyrin repeats (3 copies)
Ank PF00023 45 - 76 1.4e-08 Ankyrin repeat
Pkinase PF00069 128 - 229 1.5e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 129 - 231 1.7e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 230 - 360 1.2e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 231 - 357 2.2e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27170 FvH4_7g14180
malus_domestica MD07G1138800.v1.1
prunus_persica Prupe.2G168200_v2.0.a1
pyrus_communis pycom01g08770 pycom07g13590
rosa_chinensis RchiOBHm_Chr1g0355091
rosa_laevigata RLG00000028178
rosa_multiflora Rmu_sc0003115.1_g000031 Rmu_sc0016725.1_g000005
rosa_roxburghii Rroxscaffold_4G00300680
rosa_rugosa Rorug01G0245000
rosa_samantha Rh1AG257300 Rh1BG226600 Rh1CG240900 Rh1DG254200
rosa_wichuraiana Rw0G011220 Rw1G022690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
AccB1I GGYRCC 3 cut(s) 717, 817, 1029
AccBSI CCGCTC 1 cut(s) 462
AccII CGCG 3 cut(s) 90, 432, 951
AciI CCGC 5 cut(s) 235, 432, 460, 580, 1131
AclWI GGATC 4 cut(s) 305, 406, 920, 933
AcoI YGGCCR 1 cut(s) 1049
AcuI CTGAAG 2 cut(s) 179, 247
AcyI GRCGYC 1 cut(s) 718
AfaI GTAC 4 cut(s) 436, 885, 992, 1123
AflII CTTAAG 1 cut(s) 742
AflIII ACRYGT 1 cut(s) 1124
AgsI TTSAA 4 cut(s) 32, 568, 634, 1061
AluBI AGCT 6 cut(s) 94, 122, 143, 261, 334, 1078
AluI AGCT 6 cut(s) 94, 122, 143, 261, 334, 1078
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 4 cut(s) 305, 406, 920, 933
AoxI GGCC 2 cut(s) 232, 1049
ApeKI GCWGC 4 cut(s) 46, 168, 380, 446
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
Asp700I GAANNNNTTC 1 cut(s) 799
AspLEI GCGC 6 cut(s) 90, 432, 534, 720, 951, 953
AspS9I GGNCC 3 cut(s) 35, 232, 905
AsuC2I CCSGG 1 cut(s) 715
AsuHPI GGTGA 3 cut(s) 80, 376, 419
AvaII GGWCC 2 cut(s) 35, 905
AxyI CCTNAGG 1 cut(s) 822
BaeI ACNNNNGTAYC 2 cut(s) 426, 459
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 3 cut(s) 717, 817, 1029
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 652
BbvI GCAGC 4 cut(s) 33, 155, 392, 433
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 2 cut(s) 95, 680
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 721
BfrI CTTAAG 1 cut(s) 742
BisI GCNGC 5 cut(s) 47, 169, 235, 381, 447
BlsI GCNGC 5 cut(s) 48, 170, 236, 382, 448
Bme1390I CCNGG 1 cut(s) 715
Bme18I GGWCC 2 cut(s) 35, 905
BmgT120I GGNCC 3 cut(s) 35, 232, 905
BmiI GGNNCC 6 cut(s) 111, 233, 719, 819, 927, 1031
BmrFI CCNGG 1 cut(s) 715
BmsI GCATC 3 cut(s) 75, 164, 241
BpiI GAAGAC 1 cut(s) 652
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpmI CTGGAG 1 cut(s) 1100
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 715
BsaAI YACGTR 1 cut(s) 1125
BsaHI GRCGYC 1 cut(s) 718
BsaJI CCNNGG 1 cut(s) 556
Bse118I RCCGGY 2 cut(s) 42, 1047
Bse1I ACTGG 2 cut(s) 314, 793
Bse21I CCTNAGG 1 cut(s) 822
Bse3DI GCAATG 2 cut(s) 468, 616
BseDI CCNNGG 1 cut(s) 556
BseGI GGATG 2 cut(s) 155, 379
BseMI GCAATG 2 cut(s) 468, 616
BseMII CTCAG 2 cut(s) 165, 813
BseNI ACTGG 2 cut(s) 314, 793
BsePI GCGCGC 1 cut(s) 949
BseRI GAGGAG 1 cut(s) 1097
BseX3I CGGCCG 1 cut(s) 1049
BseXI GCAGC 4 cut(s) 33, 155, 392, 433
BseYI CCCAGC 2 cut(s) 228, 330
BsgI GTGCAG 1 cut(s) 154
Bsh1236I CGCG 3 cut(s) 90, 432, 951
Bsh1285I CGRYCG 1 cut(s) 1052
BshFI GGCC 2 cut(s) 234, 1051
BshNI GGYRCC 3 cut(s) 717, 817, 1029
BsiEI CGRYCG 1 cut(s) 1052
BsiSI CCGG 4 cut(s) 43, 113, 714, 1048
BsmAI GTCTC 1 cut(s) 15
BsmI GAATGC 1 cut(s) 1028
BsnI GGCC 2 cut(s) 234, 1051
Bsp143I GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
BspACI CCGC 5 cut(s) 235, 432, 460, 580, 1131
BspANI GGCC 2 cut(s) 234, 1051
BspCNI CTCAG 2 cut(s) 166, 814
BspFNI CGCG 3 cut(s) 90, 432, 951
BspLI GGNNCC 6 cut(s) 111, 233, 719, 819, 927, 1031
BspPI GGATC 4 cut(s) 305, 406, 920, 933
BspQI GCTCTTC 1 cut(s) 150
BspT107I GGYRCC 3 cut(s) 717, 817, 1029
BspTI CTTAAG 1 cut(s) 742
BsrBI CCGCTC 1 cut(s) 462
BsrDI GCAATG 2 cut(s) 468, 616
BsrFI RCCGGY 2 cut(s) 42, 1047
BsrI ACTGG 2 cut(s) 314, 793
BssAI RCCGGY 2 cut(s) 42, 1047
BssECI CCNNGG 1 cut(s) 556
BssHII GCGCGC 1 cut(s) 949
BssMI GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
BssNI GRCGYC 1 cut(s) 718
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 556
Bst2BI CACGAG 1 cut(s) 269
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 3 cut(s) 150, 629, 1056
BstACI GRCGYC 1 cut(s) 718
BstAFI CTTAAG 1 cut(s) 742
BstAPI GCANNNNNTGC 1 cut(s) 529
BstBAI YACGTR 1 cut(s) 1125
BstC8I GCNNGC 4 cut(s) 92, 951, 1028, 1049
BstDEI CTNAG 3 cut(s) 174, 297, 822
BstF5I GGATG 2 cut(s) 155, 379
BstFNI CGCG 3 cut(s) 90, 432, 951
BstH2I RGCGCY 1 cut(s) 721
BstHHI GCGC 6 cut(s) 90, 432, 534, 720, 951, 953
BstKTI GATC 7 cut(s) 226, 313, 355, 401, 588, 655, 928
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
BstMCI CGRYCG 1 cut(s) 1052
BstMWI GCNNNNNNNGC 2 cut(s) 234, 529
BstSCI CCNGG 1 cut(s) 713
BstSFI CTRYAG 1 cut(s) 663
BstUI CGCG 3 cut(s) 90, 432, 951
BstV1I GCAGC 4 cut(s) 33, 155, 392, 433
BstV2I GAAGAC 1 cut(s) 652
BstX2I RGATCY 2 cut(s) 310, 925
BstYI RGATCY 2 cut(s) 310, 925
BstZI CGGCCG 1 cut(s) 1049
Bsu36I CCTNAGG 1 cut(s) 822
BsuRI GGCC 2 cut(s) 234, 1051
BtsCI GGATG 2 cut(s) 155, 379
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 4 cut(s) 92, 951, 1028, 1049
CfoI GCGC 6 cut(s) 90, 432, 534, 720, 951, 953
Cfr10I RCCGGY 2 cut(s) 42, 1047
Cfr13I GGNCC 3 cut(s) 35, 232, 905
CseI GACGC 1 cut(s) 1018
Csp6I GTAC 4 cut(s) 435, 884, 991, 1122
CviAII CATG 4 cut(s) 339, 370, 623, 887
CviQI GTAC 4 cut(s) 435, 884, 991, 1122
DdeI CTNAG 3 cut(s) 174, 297, 822
DinI GGCGCC 1 cut(s) 719
DpnI GATC 7 cut(s) 225, 312, 354, 400, 587, 654, 927
DpnII GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
EaeI YGGCCR 1 cut(s) 1049
EagI CGGCCG 1 cut(s) 1049
Eam1104I CTCTTC 3 cut(s) 150, 629, 1056
EarI CTCTTC 3 cut(s) 150, 629, 1056
EclXI CGGCCG 1 cut(s) 1049
Eco130I CCWWGG 1 cut(s) 556
Eco32I GATATC 2 cut(s) 685, 862
Eco47I GGWCC 2 cut(s) 35, 905
Eco52I CGGCCG 1 cut(s) 1049
Eco57I CTGAAG 2 cut(s) 179, 247
Eco81I CCTNAGG 1 cut(s) 822
EcoRV GATATC 2 cut(s) 685, 862
EcoT14I CCWWGG 1 cut(s) 556
EgeI GGCGCC 1 cut(s) 719
EheI GGCGCC 1 cut(s) 719
ErhI CCWWGG 1 cut(s) 556
FaeI CATG 4 cut(s) 342, 373, 626, 890
FalI AAGNNNNNCTT 6 cut(s) 369, 401, 482, 514, 633, 665
FatI CATG 4 cut(s) 338, 369, 622, 886
FauNDI CATATG 3 cut(s) 403, 844, 955
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 5 cut(s) 47, 169, 235, 381, 447
FokI GGATG 2 cut(s) 142, 386
Fsp4HI GCNGC 5 cut(s) 47, 169, 235, 381, 447
FspBI CTAG 2 cut(s) 95, 680
GlaI GCGC 6 cut(s) 89, 431, 533, 719, 950, 952
GluI GCNGC 5 cut(s) 47, 169, 235, 381, 447
GsaI CCCAGC 2 cut(s) 232, 334
GsuI CTGGAG 1 cut(s) 1100
HaeII RGCGCY 1 cut(s) 721
HaeIII GGCC 2 cut(s) 234, 1051
HapII CCGG 4 cut(s) 43, 113, 714, 1048
HgaI GACGC 1 cut(s) 1018
HhaI GCGC 6 cut(s) 90, 432, 534, 720, 951, 953
Hin1I GRCGYC 1 cut(s) 718
Hin1II CATG 4 cut(s) 342, 373, 626, 890
Hin6I GCGC 6 cut(s) 88, 430, 532, 718, 949, 951
HinP1I GCGC 6 cut(s) 88, 430, 532, 718, 949, 951
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 5 cut(s) 326, 839, 962, 1036, 1087
HpaII CCGG 4 cut(s) 43, 113, 714, 1048
HphI GGTGA 3 cut(s) 80, 376, 419
Hpy166II GTNNAC 2 cut(s) 214, 367
Hpy188I TCNGA 4 cut(s) 198, 250, 902, 1017
Hpy188III TCNNGA 8 cut(s) 269, 680, 836, 929, 966, 1006, 1040, 1091
Hpy8I GTNNAC 2 cut(s) 214, 367
Hpy99I CGWCG 2 cut(s) 837, 1057
HpyAV CCTTC 3 cut(s) 26, 158, 499
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 2 cut(s) 1055, 1124
HpyCH4V TGCA 8 cut(s) 4, 171, 282, 550, 609, 622, 755, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 529
HpyF3I CTNAG 3 cut(s) 174, 297, 822
HpySE526I ACGT 2 cut(s) 1055, 1124
Hsp92I GRCGYC 1 cut(s) 718
Hsp92II CATG 4 cut(s) 342, 373, 626, 890
HspAI GCGC 6 cut(s) 88, 430, 532, 718, 949, 951
KasI GGCGCC 1 cut(s) 717
KroI GCCGGC 1 cut(s) 1047
KroNI GCCGGC 1 cut(s) 1049
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 109
Lsp1109I GCAGC 4 cut(s) 33, 155, 392, 433
LweI GCATC 3 cut(s) 75, 164, 241
MaeI CTAG 2 cut(s) 95, 680
MaeII ACGT 2 cut(s) 1055, 1124
MaeIII GTNAC 3 cut(s) 68, 266, 727
MalI GATC 7 cut(s) 225, 312, 354, 400, 587, 654, 927
MbiI CCGCTC 1 cut(s) 462
MboI GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
MflI RGATCY 2 cut(s) 310, 925
MluCI AATT 3 cut(s) 551, 750, 936
Mly113I GGCGCC 1 cut(s) 718
MmeI TCCRAC 1 cut(s) 4
MroNI GCCGGC 1 cut(s) 1047
MroXI GAANNNNTTC 1 cut(s) 799
MseI TTAA 2 cut(s) 743, 1100
MspCI CTTAAG 1 cut(s) 742
MspI CCGG 4 cut(s) 43, 113, 714, 1048
MspR9I CCNGG 1 cut(s) 715
Mva1269I GAATGC 1 cut(s) 1028
MvnI CGCG 3 cut(s) 90, 432, 951
MwoI GCNNNNNNNGC 2 cut(s) 234, 529
NaeI GCCGGC 1 cut(s) 1049
NarI GGCGCC 1 cut(s) 718
NciI CCSGG 1 cut(s) 715
NdeI CATATG 3 cut(s) 403, 844, 955
NdeII GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
NgoMIV GCCGGC 1 cut(s) 1047
NlaIII CATG 4 cut(s) 342, 373, 626, 890
NlaIV GGNNCC 6 cut(s) 111, 233, 719, 819, 927, 1031
NmuCI GTSAC 3 cut(s) 68, 266, 727
PauI GCGCGC 1 cut(s) 949
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 1 cut(s) 1028
PdiI GCCGGC 1 cut(s) 1049
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 5 cut(s) 326, 839, 962, 1036, 1087
PkrI GCNGC 5 cut(s) 48, 170, 236, 382, 448
PluTI GGCGCC 1 cut(s) 721
Ppu21I YACGTR 1 cut(s) 1125
PsiI TTATAA 1 cut(s) 780
PspFI CCCAGC 2 cut(s) 228, 330
PspN4I GGNNCC 6 cut(s) 111, 233, 719, 819, 927, 1031
PspPI GGNCC 3 cut(s) 35, 232, 905
PsuI RGATCY 2 cut(s) 310, 925
PteI GCGCGC 1 cut(s) 949
RsaI GTAC 4 cut(s) 436, 885, 992, 1123
RsaNI GTAC 4 cut(s) 435, 884, 991, 1122
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 2 cut(s) 743, 1100
SatI GCNGC 5 cut(s) 47, 169, 235, 381, 447
Sau3AI GATC 7 cut(s) 223, 310, 352, 398, 585, 652, 925
Sau96I GGNCC 3 cut(s) 35, 232, 905
ScrFI CCNGG 1 cut(s) 715
SfaNI GCATC 3 cut(s) 75, 164, 241
SfcI CTRYAG 1 cut(s) 663
SfoI GGCGCC 1 cut(s) 719
SinI GGWCC 2 cut(s) 35, 905
SmlI CTYRAG 2 cut(s) 722, 742
SmoI CTYRAG 2 cut(s) 722, 742
Sse9I AATT 3 cut(s) 551, 750, 936
SsiI CCGC 5 cut(s) 235, 432, 460, 580, 1131
SspDI GGCGCC 1 cut(s) 717
SspMI CTAG 2 cut(s) 95, 680
StyD4I CCNGG 1 cut(s) 713
StyI CCWWGG 1 cut(s) 556
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 2 cut(s) 1058, 1127
TaqI TCGA 4 cut(s) 59, 256, 835, 967
TasI AATT 3 cut(s) 551, 750, 936
TatI WGTACW 1 cut(s) 883
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 5 cut(s) 326, 839, 962, 1036, 1087
Tru1I TTAA 2 cut(s) 743, 1100
Tru9I TTAA 2 cut(s) 743, 1100
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 3 cut(s) 68, 266, 727
TseI GCWGC 4 cut(s) 46, 168, 380, 446
Tsp45I GTSAC 3 cut(s) 68, 266, 727
TspDTI ATGAA 5 cut(s) 360, 390, 518, 723, 792
TspGWI ACGGA 1 cut(s) 746
TspRI CASTG 1 cut(s) 178
Vha464I CTTAAG 1 cut(s) 742
VpaK11BI GGWCC 2 cut(s) 35, 905
XbaI TCTAGA 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 2 cut(s) 95, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.