RchiOBHm_Chr1g0355091

Seven transmembrane domain-containing tyrosine-protein kinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0013645
Physical Location & Seq
Reverse (-)
47851013 .. 47853921
2909 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58059

Sequence Viewer

Length: 1224 bp
ATGCAGAAGCCTAAAAAGTTCACCTCAGAAGGTCCATACCGGCTGTTATATTGTTCAAGTAAGGGTGACAAAGCAGGGGTGGAGCAGGAGTTAGAGAAAGGTGTGGAGCCGAATCTTGGGGACTATGATAAGAGAACAGCTCTTCATTTGGCATCATGTGAAGGCTGCACTGAAATTGTTGTTCTACTTCTTCAGAAAGGTGCTAATGTTAACTGCACAGACCGCTGGGGCCGCACTCCATTGTCTGATGCTCGTAGCTTCGCTCATGATGGGATTTGCAAGATATTGGAGGCTAGGGGTGGAATAGATCCGGTAGGGCTTGACTCTCAGCTTCCATGCTATGAAATTTCTTACAAAGAGGTGAACATGGACGAGGCAACACTTATTGGAGAGGGAGCATATGGTGAAATTTATTTAGTAAAGTGGCGTGGTACAGAAGTTGCTGCTAAAACAATTCGTCCCTCCATCGCTTCTAAACCTACTGTGAAGGCTCTCTTTCTGAAGGAACTTGCTTTGTGGCAACAGTTGCGTCACCCTAACATAGTGCAATTCCTTGGGGTCATGAAGCACCCTGATCGCTTGATCTTCCTTACAGAGTTTCTTTGCAATGGAAGTTTGCATGATATATTGAAAAGGAAGGGAAGACTTGATCTGAAAACCGTCGTTGGTTATGCTTTAGATATTGCCAGAGGTATGAATTATCTCCATCAGCATAAACCGCGCCCTATAATCCATAGAGATCTTACACCAAGAAATGTGTTACAAGATGAAGCTGGACGACTCAAGGTCACAGATTTTGGGTTAAGCAAAATCGCCCAACACAATGATGTATCTGGTTATAAAATGACTGGAAGGACAGGCTCATATCGGTATATGGCACCTGAGGTTTATCGTCGAGAATCATATGGGAAGAGTATCGATATCTTCTCATTTGCTCTATTGGTACATGAGATGTTTCTAGGAGGACCACCAAATCTGGCAGAGGATCCTGAACAAATTGCAGACAAGAGAGCATATGAAGATTCAAGACCACCTCTCTACTCTTATTTGTTCCCGGAACCAATTCGAAAGCTTCTTAAAGAATGCTGGCACCCGAATCCAGATTGCCGACCTACATTCGAAGAGATCATTGTGGAGTTGGAGTCTATTCAAGGGTCTATTCAGGACAGCTTTCATTTCAAGAAAAGATTGGGAACCTGTAATATTAGGTGTGTCATCTCTTAA

Protein Analysis

407

Amino Acids

46.11

Weight (kDa)

8.36

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 102 8.1e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 32 - 81 2.6e-06 Ankyrin repeats (many copies)
Ank PF00023 44 - 75 1.3e-08 Ankyrin repeat
Pkinase PF00069 127 - 377 2.8e-52 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 128 - 380 1.1e-53 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27170 FvH4_7g14180
malus_domestica MD07G1138800.v1.1
prunus_persica Prupe.2G168200_v2.0.a1
pyrus_communis pycom01g08770 pycom07g13590
rosa_chinensis RchiOBHm_Chr1g0355091
rosa_laevigata RLG00000028178
rosa_multiflora Rmu_sc0003115.1_g000031 Rmu_sc0016725.1_g000005
rosa_roxburghii Rroxscaffold_4G00300680
rosa_rugosa Rorug01G0245000
rosa_samantha Rh1AG257300 Rh1BG226600 Rh1CG240900 Rh1DG254200
rosa_wichuraiana Rw0G011220 Rw1G022690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 877, 1089
AccII CGCG 1 cut(s) 721
AciI CCGC 3 cut(s) 223, 232, 719
AclWI GGATC 3 cut(s) 302, 980, 993
AcsI RAATTY 2 cut(s) 345, 408
AcuI CTGAAG 2 cut(s) 176, 521
AfaI GTAC 2 cut(s) 433, 945
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 5 cut(s) 57, 631, 1026, 1151, 1180
AhdI GACNNNNNGTC 1 cut(s) 785
AluBI AGCT 6 cut(s) 140, 258, 331, 773, 1072, 1170
AluI AGCT 6 cut(s) 140, 258, 331, 773, 1072, 1170
AlwI GGATC 3 cut(s) 302, 980, 993
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 2 cut(s) 165, 443
ApoI RAATTY 2 cut(s) 345, 408
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 1 cut(s) 723
AspS9I GGNCC 3 cut(s) 32, 229, 965
AsuC2I CCSGG 1 cut(s) 1055
AsuHPI GGTGA 5 cut(s) 13, 77, 373, 416, 524
AsuII TTCGAA 2 cut(s) 1066, 1119
AvaII GGWCC 2 cut(s) 32, 965
AxyI CCTNAGG 1 cut(s) 882
BaeI ACNNNNGTAYC 2 cut(s) 423, 456
BamHI GGATCC 1 cut(s) 985
BanI GGYRCC 2 cut(s) 877, 1089
BbsI GAAGAC 1 cut(s) 649
BbvI GCAGC 2 cut(s) 152, 430
BccI CCATC 3 cut(s) 263, 473, 714
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BcnI CCSGG 1 cut(s) 1055
BfaI CTAG 2 cut(s) 294, 959
BglII AGATCT 1 cut(s) 739
BisI GCNGC 3 cut(s) 166, 232, 444
BlsI GCNGC 3 cut(s) 167, 233, 445
Bme1390I CCNGG 1 cut(s) 1055
Bme18I GGWCC 2 cut(s) 32, 965
BmeRI GACNNNNNGTC 1 cut(s) 785
BmgT120I GGNCC 3 cut(s) 32, 229, 965
BmiI GGNNCC 7 cut(s) 108, 230, 879, 987, 1059, 1091, 1195
BmrFI CCNGG 1 cut(s) 1055
BmsI GCATC 2 cut(s) 161, 238
BpiI GAAGAC 1 cut(s) 649
BplI GAGNNNNNCTC 2 cut(s) 124, 156
Bpu14I TTCGAA 2 cut(s) 1066, 1119
BpuEI CTTGAG 1 cut(s) 767
BpuMI CCSGG 1 cut(s) 1055
Bsa29I ATCGAT 1 cut(s) 918
BsaJI CCNNGG 1 cut(s) 553
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 1127, 1157
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse118I RCCGGY 1 cut(s) 39
Bse1I ACTGG 1 cut(s) 853
Bse21I CCTNAGG 1 cut(s) 882
Bse3DI GCAATG 1 cut(s) 613
BseCI ATCGAT 1 cut(s) 918
BseDI CCNNGG 1 cut(s) 553
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 3 cut(s) 39, 341, 873
BseNI ACTGG 1 cut(s) 853
BseXI GCAGC 2 cut(s) 152, 430
BseYI CCCAGC 1 cut(s) 225
BsgI GTGCAG 2 cut(s) 151, 199
Bsh1236I CGCG 1 cut(s) 721
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 2 cut(s) 877, 1089
BshVI ATCGAT 1 cut(s) 918
BsiSI CCGG 3 cut(s) 40, 311, 1055
BslFI GGGAC 2 cut(s) 134, 444
BslI CCNNNNNNNGG 1 cut(s) 116
BsmFI GGGAC 2 cut(s) 134, 444
BsmI GAATGC 1 cut(s) 1088
BsnI GGCC 1 cut(s) 231
Bsp119I TTCGAA 2 cut(s) 1066, 1119
Bsp143I GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
BspACI CCGC 3 cut(s) 223, 232, 719
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 3 cut(s) 38, 340, 874
BspDI ATCGAT 1 cut(s) 918
BspFNI CGCG 1 cut(s) 721
BspHI TCATGA 2 cut(s) 265, 561
BspLI GGNNCC 7 cut(s) 108, 230, 879, 987, 1059, 1091, 1195
BspPI GGATC 3 cut(s) 302, 980, 993
BspQI GCTCTTC 1 cut(s) 147
BspT104I TTCGAA 2 cut(s) 1066, 1119
BspT107I GGYRCC 2 cut(s) 877, 1089
BsrDI GCAATG 1 cut(s) 613
BsrFI RCCGGY 1 cut(s) 39
BsrI ACTGG 1 cut(s) 853
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
BssT1I CCWWGG 1 cut(s) 553
Bst4CI ACNGT 3 cut(s) 484, 525, 661
Bst6I CTCTTC 3 cut(s) 147, 905, 1116
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBI TTCGAA 2 cut(s) 1066, 1119
BstC8I GCNNGC 1 cut(s) 1088
BstDEI CTNAG 3 cut(s) 25, 327, 882
BstFNI CGCG 1 cut(s) 721
BstHHI GCGC 1 cut(s) 723
BstKTI GATC 7 cut(s) 310, 577, 585, 652, 742, 988, 1128
BstMBI GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
BstMWI GCNNNNNNNGC 4 cut(s) 222, 231, 526, 718
BstSCI CCNGG 1 cut(s) 1053
BstUI CGCG 1 cut(s) 721
BstV1I GCAGC 2 cut(s) 152, 430
BstV2I GAAGAC 1 cut(s) 649
BstX2I RGATCY 3 cut(s) 307, 739, 985
BstYI RGATCY 3 cut(s) 307, 739, 985
Bsu15I ATCGAT 1 cut(s) 918
Bsu36I CCTNAGG 1 cut(s) 882
BsuRI GGCC 1 cut(s) 231
BsuTUI ATCGAT 1 cut(s) 918
BtgZI GCGATG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 1088
CciI TCATGA 2 cut(s) 265, 561
CfoI GCGC 1 cut(s) 723
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 3 cut(s) 32, 229, 965
ClaI ATCGAT 1 cut(s) 918
CseI GACGC 1 cut(s) 518
Csp6I GTAC 2 cut(s) 432, 944
CviAII CATG 7 cut(s) 156, 266, 336, 367, 562, 620, 947
CviQI GTAC 2 cut(s) 432, 944
DdeI CTNAG 3 cut(s) 25, 327, 882
DpnI GATC 7 cut(s) 309, 576, 584, 651, 741, 987, 1127
DpnII GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
DriI GACNNNNNGTC 1 cut(s) 785
Eam1104I CTCTTC 3 cut(s) 147, 905, 1116
Eam1105I GACNNNNNGTC 1 cut(s) 785
EarI CTCTTC 3 cut(s) 147, 905, 1116
Eco130I CCWWGG 1 cut(s) 553
Eco32I GATATC 1 cut(s) 922
Eco47I GGWCC 2 cut(s) 32, 965
Eco57I CTGAAG 2 cut(s) 176, 521
Eco81I CCTNAGG 1 cut(s) 882
EcoRV GATATC 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 553
FaeI CATG 7 cut(s) 159, 269, 339, 370, 565, 623, 950
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 2 cut(s) 134, 444
FatI CATG 7 cut(s) 155, 265, 335, 366, 561, 619, 946
FauNDI CATATG 3 cut(s) 400, 904, 1015
Fnu4HI GCNGC 3 cut(s) 166, 232, 444
Fsp4HI GCNGC 3 cut(s) 166, 232, 444
FspBI CTAG 2 cut(s) 294, 959
GlaI GCGC 1 cut(s) 722
GluI GCNGC 3 cut(s) 166, 232, 444
GsaI CCCAGC 1 cut(s) 229
HaeIII GGCC 1 cut(s) 231
HapII CCGG 3 cut(s) 40, 311, 1055
HgaI GACGC 1 cut(s) 518
HhaI GCGC 1 cut(s) 723
Hin1II CATG 7 cut(s) 159, 269, 339, 370, 565, 623, 950
Hin6I GCGC 1 cut(s) 721
HinP1I GCGC 1 cut(s) 721
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 1070
HinfI GANTC 7 cut(s) 112, 323, 780, 899, 1022, 1096, 1142
HpaI GTTAAC 1 cut(s) 211
HpaII CCGG 3 cut(s) 40, 311, 1055
HphI GGTGA 5 cut(s) 13, 77, 373, 416, 524
Hpy166II GTNNAC 3 cut(s) 21, 211, 364
Hpy188I TCNGA 5 cut(s) 28, 195, 247, 501, 654
Hpy188III TCNNGA 8 cut(s) 266, 562, 896, 989, 1026, 1100, 1163, 1180
Hpy8I GTNNAC 3 cut(s) 21, 211, 364
Hpy99I CGWCG 2 cut(s) 665, 897
HpyAV CCTTC 6 cut(s) 23, 155, 481, 496, 631, 846
HpyCH4III ACNGT 3 cut(s) 484, 525, 661
HpyCH4V TGCA 8 cut(s) 4, 168, 216, 279, 547, 606, 619, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 222, 231, 526, 718
HpyF3I CTNAG 3 cut(s) 25, 327, 882
Hsp92II CATG 7 cut(s) 159, 269, 339, 370, 565, 623, 950
HspAI GCGC 1 cut(s) 721
KspAI GTTAAC 1 cut(s) 211
Kzo9I GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 3 cut(s) 82, 106, 395
Lsp1109I GCAGC 2 cut(s) 152, 430
LweI GCATC 2 cut(s) 161, 238
MaeI CTAG 2 cut(s) 294, 959
MaeIII GTNAC 4 cut(s) 65, 530, 759, 787
MalI GATC 7 cut(s) 309, 576, 584, 651, 741, 987, 1127
MboI GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
MboII GAAGA 8 cut(s) 134, 182, 577, 654, 916, 922, 1031, 1133
MflI RGATCY 3 cut(s) 307, 739, 985
MluCI AATT 8 cut(s) 174, 345, 408, 453, 548, 697, 996, 1062
MlyI GAGTC 3 cut(s) 317, 774, 1151
MmeI TCCRAC 1 cut(s) 1119
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 4 cut(s) 210, 803, 1077, 1222
MslI CAYNNNNRTG 1 cut(s) 825
MspA1I CMGCKG 1 cut(s) 225
MspI CCGG 3 cut(s) 40, 311, 1055
MspR9I CCNGG 1 cut(s) 1055
Mva1269I GAATGC 1 cut(s) 1088
MvnI CGCG 1 cut(s) 721
MwoI GCNNNNNNNGC 4 cut(s) 222, 231, 526, 718
NciI CCSGG 1 cut(s) 1055
NdeI CATATG 3 cut(s) 400, 904, 1015
NdeII GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
NlaIII CATG 7 cut(s) 159, 269, 339, 370, 565, 623, 950
NlaIV GGNNCC 7 cut(s) 108, 230, 879, 987, 1059, 1091, 1195
NmuCI GTSAC 3 cut(s) 65, 530, 787
NspV TTCGAA 2 cut(s) 1066, 1119
PagI TCATGA 2 cut(s) 265, 561
PciSI GCTCTTC 1 cut(s) 147
PctI GAATGC 1 cut(s) 1088
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 4 cut(s) 112, 899, 1022, 1096
PfoI TCCNGGA 1 cut(s) 1053
PkrI GCNGC 3 cut(s) 167, 233, 445
PleI GAGTC 3 cut(s) 317, 774, 1150
PpsI GAGTC 3 cut(s) 317, 774, 1150
PsiI TTATAA 1 cut(s) 840
PspFI CCCAGC 1 cut(s) 225
PspN4I GGNNCC 7 cut(s) 108, 230, 879, 987, 1059, 1091, 1195
PspPI GGNCC 3 cut(s) 32, 229, 965
PsuI RGATCY 3 cut(s) 307, 739, 985
RsaI GTAC 2 cut(s) 433, 945
RsaNI GTAC 2 cut(s) 432, 944
RseI CAYNNNNRTG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 4 cut(s) 210, 803, 1077, 1222
SatI GCNGC 3 cut(s) 166, 232, 444
Sau3AI GATC 7 cut(s) 307, 574, 582, 649, 739, 985, 1125
Sau96I GGNCC 3 cut(s) 32, 229, 965
SchI GAGTC 3 cut(s) 317, 774, 1151
ScrFI CCNGG 1 cut(s) 1055
SfaNI GCATC 2 cut(s) 161, 238
SfuI TTCGAA 2 cut(s) 1066, 1119
SinI GGWCC 2 cut(s) 32, 965
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 8 cut(s) 174, 345, 408, 453, 548, 697, 996, 1062
SsiI CCGC 3 cut(s) 223, 232, 719
SspI AATATT 1 cut(s) 1204
SspMI CTAG 2 cut(s) 294, 959
StyD4I CCNGG 1 cut(s) 1053
StyI CCWWGG 1 cut(s) 553
TaaI ACNGT 3 cut(s) 484, 525, 661
TaqI TCGA 4 cut(s) 895, 918, 1066, 1119
TasI AATT 8 cut(s) 174, 345, 408, 453, 548, 697, 996, 1062
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 4 cut(s) 112, 899, 1022, 1096
Tru1I TTAA 4 cut(s) 210, 803, 1077, 1222
Tru9I TTAA 4 cut(s) 210, 803, 1077, 1222
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 3 cut(s) 65, 530, 787
TseI GCWGC 2 cut(s) 165, 443
Tsp45I GTSAC 3 cut(s) 65, 530, 787
TspDTI ATGAA 7 cut(s) 134, 357, 578, 710, 783, 1032, 1163
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 2 cut(s) 32, 965
XapI RAATTY 2 cut(s) 345, 408
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 2 cut(s) 294, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.