MD07G1150900.v1.1

DNA metabolic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012649
Physical Location & Seq
Reverse (-)
21907460 .. 21910835
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1150900.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGTCAAAACCCAACCACCAGAATAATCTAGAAATCAAGCGGCACGAGGATAATATTAAGTTCCTTCAGAATGAGATAAACTGCTTAGGTGAATCTATACTGGAATTGCAAGTGAGTCTTGCAAAGCATCAGTCGGCCAATGGTACTGTAACAACAAATGAAAACGGTCCTACTGCTGAGGAGGAAGACCAAATATTGCGGCACGAAAAATCCGCGGCTGGCTTATTGTGCAGGCTGAAACTGTTAAAATCGCAGCATGCGAGTCAGGCATTGAATTTGGCTCTGACAAAGGATGTGCTGGGTATTGTTGGCATTGTTGCCACACTTGGCAGAGTCGAGGATGACAACCTTAGCAGGCTTCTGTCGGAGTACTTGGGACTGGAAACTATGCTAGCAATTGTATGCAGGACAAGTGAAGCCGTTATGGTTCTAGAGAAGTATGATGGGGATGGGACAGTACTCACCAGCGCTGGCATACATGGTCTAGGAGTTTCTATTGGGAAGAGCATAAAAGGGCGGTTTCTGGTCATATGTCTAGATGATTTGAGTCCATATGTTGGGGGATTTGTAGCTGATGATCCACAAAGGAAGCTTGCTCTTCCCAAACCACAGCTACCAAATGGGGAGTGTCCGCGCGGCTTTCTTGATTATGCAGTGAACACGATCAATTTGGACGGCAATAACTTAGTTAATGTTACCAGCAGTGGGCATGGTCTCAGAGAGACACTATTCTATAGTCTCTTTTCTCGCCTCCAAATATACAGAACCAGAGCAGAGATGCTTCTCGCTCTCCCATGCATAAATCATGGAGCACTGTCTTTAGATGGCGGGATTATTAAAAAGAGTGGCGTGTTCATCCTGGGAAGCAGCAAAGATACAGAGATCAAATTTCTTGCAAAATCCGGAGAATCTAGTATGACTGCAAACTACGACGAAACTGAGGATATGATCAAGAAGCTGAAGTTCAAGCGAAAGCATGTTGCTGAGGATATGCAAAGAGAACAAGAATTGTTGGACTTCGCAAAGGCAAACCTGGCACAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

349

Amino Acids

38.16

Weight (kDa)

6.03

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 557
AccII CGCG 3 cut(s) 215, 634, 636
AccIII TCCGGA 1 cut(s) 902
AciI CCGC 8 cut(s) 40, 199, 213, 215, 517, 632, 636, 828
AclWI GGATC 1 cut(s) 572
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 2 cut(s) 274, 887
AcuI CTGAAG 2 cut(s) 50, 980
AfaI GTAC 3 cut(s) 145, 371, 459
AfeI AGCGCT 1 cut(s) 469
AfiI CCNNNNNNNGG 2 cut(s) 557, 705
AgsI TTSAA 2 cut(s) 274, 967
AjnI CCWGG 2 cut(s) 858, 1032
AleI CACNNNNGTG 1 cut(s) 1042
AluBI AGCT 4 cut(s) 572, 592, 613, 958
AluI AGCT 4 cut(s) 572, 592, 613, 958
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 3 cut(s) 716, 719, 743
AlwI GGATC 1 cut(s) 572
Aor13HI TCCGGA 1 cut(s) 902
Aor51HI AGCGCT 1 cut(s) 469
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 253, 867
ApoI RAATTY 2 cut(s) 274, 887
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AspLEI GCGC 2 cut(s) 470, 636
AspS9I GGNCC 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 101, 454
AsuNHI GCTAGC 1 cut(s) 391
AvaII GGWCC 1 cut(s) 167
BauI CACGAG 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 814
BbvCI CCTCAGC 2 cut(s) 177, 984
BbvI GCAGC 2 cut(s) 265, 879
BccI CCATC 3 cut(s) 437, 443, 818
BceAI ACGGC 2 cut(s) 404, 691
BciT130I CCWGG 2 cut(s) 860, 1034
BclI TGATCA 1 cut(s) 948
BcoDI GTCTC 3 cut(s) 716, 719, 743
BfaI CTAG 6 cut(s) 29, 392, 431, 485, 536, 912
BfmI CTRYAG 1 cut(s) 733
BfoI RGCGCY 1 cut(s) 471
BisI GCNGC 6 cut(s) 41, 200, 216, 254, 637, 868
BlsI GCNGC 6 cut(s) 42, 201, 217, 255, 638, 869
BmcAI AGTACT 2 cut(s) 371, 459
Bme1390I CCNGG 2 cut(s) 860, 1034
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmrFI CCNGG 2 cut(s) 860, 1034
BmsI GCATC 2 cut(s) 136, 768
BmtI GCTAGC 1 cut(s) 395
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 4 cut(s) 85, 177, 350, 984
BsaI GGTCTC 1 cut(s) 719
BsaJI CCNNGG 2 cut(s) 213, 859
BsaWI WCCGGW 1 cut(s) 902
Bsc4I CCNNNNNNNGG 2 cut(s) 557, 705
Bse1I ACTGG 2 cut(s) 105, 384
BseAI TCCGGA 1 cut(s) 902
BseBI CCWGG 2 cut(s) 860, 1034
BseDI CCNNGG 2 cut(s) 213, 859
BseGI GGATG 4 cut(s) 298, 346, 454, 855
BseLI CCNNNNNNNGG 2 cut(s) 557, 705
BseMII CTCAG 4 cut(s) 168, 730, 930, 975
BseNI ACTGG 2 cut(s) 105, 384
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 2 cut(s) 265, 879
BseYI CCCAGC 1 cut(s) 298
BsgI GTGCAG 1 cut(s) 250
Bsh1236I CGCG 3 cut(s) 215, 634, 636
BshFI GGCC 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 1 cut(s) 903
BslFI GGGAC 2 cut(s) 390, 466
BslI CCNNNNNNNGG 2 cut(s) 557, 705
BsmAI GTCTC 3 cut(s) 716, 719, 743
BsmFI GGGAC 2 cut(s) 390, 466
BsnI GGCC 1 cut(s) 137
Bso31I GGTCTC 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 814
Bsp13I TCCGGA 1 cut(s) 902
Bsp143I GATC 4 cut(s) 577, 663, 882, 948
BspACI CCGC 8 cut(s) 40, 199, 213, 215, 517, 632, 636, 828
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 4 cut(s) 169, 729, 931, 976
BspEI TCCGGA 1 cut(s) 902
BspFNI CGCG 3 cut(s) 215, 634, 636
BspOI GCTAGC 1 cut(s) 395
BspPI GGATC 1 cut(s) 572
BspQI GCTCTTC 2 cut(s) 497, 603
BspTNI GGTCTC 1 cut(s) 719
BsrI ACTGG 2 cut(s) 105, 384
BssECI CCNNGG 2 cut(s) 213, 859
BssMI GATC 4 cut(s) 577, 663, 882, 948
BssSI CACGAG 1 cut(s) 44
Bst2BI CACGAG 1 cut(s) 44
Bst2UI CCWGG 2 cut(s) 860, 1034
Bst4CI ACNGT 5 cut(s) 148, 167, 243, 457, 816
Bst6I CTCTTC 2 cut(s) 497, 603
BstC8I GCNNGC 7 cut(s) 220, 233, 258, 356, 393, 472, 594
BstDEI CTNAG 7 cut(s) 85, 177, 350, 685, 716, 939, 984
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 4 cut(s) 298, 346, 454, 855
BstFNI CGCG 3 cut(s) 215, 634, 636
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 2 cut(s) 470, 636
BstKTI GATC 4 cut(s) 580, 666, 885, 951
BstMAI GTCTC 3 cut(s) 716, 719, 743
BstMBI GATC 4 cut(s) 577, 663, 882, 948
BstMWI GCNNNNNNNGC 3 cut(s) 228, 266, 1034
BstNI CCWGG 2 cut(s) 860, 1034
BstNSI RCATGY 2 cut(s) 260, 980
BstSCI CCNGG 2 cut(s) 858, 1032
BstSFI CTRYAG 1 cut(s) 733
BstUI CGCG 3 cut(s) 215, 634, 636
BstV1I GCAGC 2 cut(s) 265, 879
BstV2I GAAGAC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 213
BtsCI GGATG 4 cut(s) 298, 346, 454, 855
BtsI GCAGTG 2 cut(s) 660, 709
BtsIMutI CAGTG 3 cut(s) 660, 709, 812
Cac8I GCNNGC 7 cut(s) 220, 233, 258, 356, 393, 472, 594
CfoI GCGC 2 cut(s) 470, 636
Cfr13I GGNCC 1 cut(s) 167
Cfr42I CCGCGG 1 cut(s) 216
Csp6I GTAC 3 cut(s) 144, 370, 458
CviAII CATG 6 cut(s) 257, 479, 710, 795, 806, 977
CviQI GTAC 3 cut(s) 144, 370, 458
DdeI CTNAG 7 cut(s) 85, 177, 350, 685, 716, 939, 984
DpnI GATC 4 cut(s) 579, 665, 884, 950
DpnII GATC 4 cut(s) 577, 663, 882, 948
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 2 cut(s) 497, 603
EarI CTCTTC 2 cut(s) 497, 603
Eco31I GGTCTC 1 cut(s) 719
Eco47I GGWCC 1 cut(s) 167
Eco47III AGCGCT 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 50, 980
EcoRII CCWGG 2 cut(s) 858, 1032
EcoT22I ATGCAT 1 cut(s) 800
FaeI CATG 6 cut(s) 260, 482, 713, 798, 809, 980
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FaqI GGGAC 2 cut(s) 390, 466
FatI CATG 6 cut(s) 256, 478, 709, 794, 805, 976
FauI CCCGC 1 cut(s) 821
FauNDI CATATG 2 cut(s) 530, 553
FbaI TGATCA 1 cut(s) 948
Fnu4HI GCNGC 6 cut(s) 41, 200, 216, 254, 637, 868
FokI GGATG 4 cut(s) 305, 353, 461, 842
Fsp4HI GCNGC 6 cut(s) 41, 200, 216, 254, 637, 868
FspBI CTAG 6 cut(s) 29, 392, 431, 485, 536, 912
GlaI GCGC 2 cut(s) 469, 635
GluI GCNGC 6 cut(s) 41, 200, 216, 254, 637, 868
GsaI CCCAGC 1 cut(s) 302
HaeII RGCGCY 1 cut(s) 471
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 903
HhaI GCGC 2 cut(s) 470, 636
Hin1II CATG 6 cut(s) 260, 482, 713, 798, 809, 980
Hin6I GCGC 2 cut(s) 468, 634
HinP1I GCGC 2 cut(s) 468, 634
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 6 cut(s) 92, 115, 262, 333, 547, 908
HpaII CCGG 1 cut(s) 903
HphI GGTGA 2 cut(s) 101, 454
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 4 cut(s) 69, 285, 367, 719
Hpy188III TCNNGA 6 cut(s) 29, 431, 536, 644, 903, 952
Hpy8I GTNNAC 1 cut(s) 658
Hpy99I CGWCG 1 cut(s) 935
HpyAV CCTTC 1 cut(s) 74
HpyCH4III ACNGT 5 cut(s) 148, 167, 243, 457, 816
HpyCH4V TGCA 9 cut(s) 109, 122, 231, 405, 653, 798, 896, 923, 994
HpyF10VI GCNNNNNNNGC 3 cut(s) 228, 266, 1034
HpyF3I CTNAG 7 cut(s) 85, 177, 350, 685, 716, 939, 984
Hsp92II CATG 6 cut(s) 260, 482, 713, 798, 809, 980
HspAI GCGC 2 cut(s) 468, 634
Kpn2I TCCGGA 1 cut(s) 902
Ksp22I TGATCA 1 cut(s) 948
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 4 cut(s) 577, 663, 882, 948
LguI GCTCTTC 2 cut(s) 497, 603
LmnI GCTCC 1 cut(s) 809
Lsp1109I GCAGC 2 cut(s) 265, 879
LweI GCATC 2 cut(s) 136, 768
MaeI CTAG 6 cut(s) 29, 392, 431, 485, 536, 912
MaeIII GTNAC 2 cut(s) 148, 694
MalI GATC 4 cut(s) 579, 665, 884, 950
MboI GATC 4 cut(s) 577, 663, 882, 948
MboII GAAGA 3 cut(s) 197, 514, 590
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 814
MluCI AATT 6 cut(s) 104, 274, 396, 667, 887, 1007
MlyI GAGTC 4 cut(s) 124, 271, 342, 556
MmeI TCCRAC 2 cut(s) 345, 993
MnlI CCTC 7 cut(s) 40, 172, 175, 331, 761, 934, 979
Mph1103I ATGCAT 1 cut(s) 800
MroI TCCGGA 1 cut(s) 902
MseI TTAA 4 cut(s) 57, 245, 690, 837
MslI CAYNNNNRTG 1 cut(s) 1042
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 903
MspR9I CCNGG 2 cut(s) 860, 1034
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 2 cut(s) 860, 1034
MvnI CGCG 3 cut(s) 215, 634, 636
MwoI GCNNNNNNNGC 3 cut(s) 228, 266, 1034
NdeI CATATG 2 cut(s) 530, 553
NdeII GATC 4 cut(s) 577, 663, 882, 948
NheI GCTAGC 1 cut(s) 391
NlaIII CATG 6 cut(s) 260, 482, 713, 798, 809, 980
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 2 cut(s) 260, 980
OliI CACNNNNGTG 1 cut(s) 1042
PaeI GCATGC 1 cut(s) 260
PciSI GCTCTTC 2 cut(s) 497, 603
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PfeI GAWTC 2 cut(s) 92, 908
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 6 cut(s) 42, 201, 217, 255, 638, 869
PleI GAGTC 4 cut(s) 123, 270, 341, 555
PpsI GAGTC 4 cut(s) 123, 270, 341, 555
Psp6I CCWGG 2 cut(s) 858, 1032
PspFI CCCAGC 1 cut(s) 298
PspGI CCWGG 2 cut(s) 858, 1032
PspPI GGNCC 1 cut(s) 167
RsaI GTAC 3 cut(s) 145, 371, 459
RsaNI GTAC 3 cut(s) 144, 370, 458
RseI CAYNNNNRTG 1 cut(s) 1042
SacII CCGCGG 1 cut(s) 216
SapI GCTCTTC 2 cut(s) 497, 603
SaqAI TTAA 4 cut(s) 57, 245, 690, 837
SatI GCNGC 6 cut(s) 41, 200, 216, 254, 637, 868
Sau3AI GATC 4 cut(s) 577, 663, 882, 948
Sau96I GGNCC 1 cut(s) 167
ScaI AGTACT 2 cut(s) 371, 459
SchI GAGTC 4 cut(s) 124, 271, 342, 556
ScrFI CCNGG 2 cut(s) 860, 1034
SduI GDGCHC 1 cut(s) 814
SetI ASST 7 cut(s) 91, 351, 574, 594, 615, 960, 1035
SfaNI GCATC 2 cut(s) 136, 768
SfcI CTRYAG 1 cut(s) 733
Sfr303I CCGCGG 1 cut(s) 216
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 1042
SphI GCATGC 1 cut(s) 260
Sse9I AATT 6 cut(s) 104, 274, 396, 667, 887, 1007
SsiI CCGC 8 cut(s) 40, 199, 213, 215, 517, 632, 636, 828
SspI AATATT 2 cut(s) 55, 195
SspMI CTAG 6 cut(s) 29, 392, 431, 485, 536, 912
StyD4I CCNGG 2 cut(s) 858, 1032
TaaI ACNGT 5 cut(s) 148, 167, 243, 457, 816
TaqI TCGA 1 cut(s) 336
TasI AATT 6 cut(s) 104, 274, 396, 667, 887, 1007
TatI WGTACW 2 cut(s) 369, 457
TauI GCSGC 4 cut(s) 43, 202, 218, 639
TfiI GAWTC 2 cut(s) 92, 908
Tru1I TTAA 4 cut(s) 57, 245, 690, 837
Tru9I TTAA 4 cut(s) 57, 245, 690, 837
TscAI CASTG 3 cut(s) 660, 709, 819
TseI GCWGC 2 cut(s) 253, 867
TspDTI ATGAA 2 cut(s) 174, 844
TspRI CASTG 3 cut(s) 660, 709, 819
Van91I CCANNNNNTGG 1 cut(s) 557
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 2 cut(s) 274, 887
XbaI TCTAGA 3 cut(s) 28, 430, 535
XceI RCATGY 2 cut(s) 260, 980
XspI CTAG 6 cut(s) 29, 392, 431, 485, 536, 912
ZrmI AGTACT 2 cut(s) 371, 459
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.