MD07G1154500.v1.1

Arginine serine-rich-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008531
Physical Location & Seq
Reverse (-)
22415788 .. 22417203
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1154500.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCAGCTTATGAGTTTTGCTTTTGTCTATTTTGAGGATGAACAGGATGCTGAAGACGCTATCTGTGGAATGACAGGCGCAGGCTATCTGTGGAATGGGCTAAGTCATACAGGTGAACGTGGTCGCCATCATGATGGGTCTAAATCACTGGCAAACCAGAGGCCGACTAAAAGTTTGTTTGTTATCATCTTTTATCCCGTCCACACCAAAGTTCGTGATATTGAAAGGCACTTTGAGCCTTATGGGAAGGTGCTTCATGTCAGAATTCGTCGGAACTTTGCATTTGTGCAGTTTGACACGCAGGAAGAAGCTACTGAAGCTCTTCAGGCTACACATTTGAGGTTAAATAGTGAGAGAGGTGATGTCGAGAGAGGTGATCGCTATTGTGATAGCCCTCCTAGAAGGGGCAGTTACGGTAGGCATGGTGATAGTCCTTATTGGAGATCACCAAGTCCTGGTTGTCGCAGGCGCCCAAATCCTGATTACGGTTGTCCTCGCAGCCCTGTTTATGACAGGTACAACGGTCCAGTGTATGACAGGCGCAGGAGTCCTGAACGTGGAAGGAATGTGAGTCCTGAATACGGCAGATACCGCAGTCGCTCGCCTATCCAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05168 map03040 map05168
Pfam Domains
Protein Families
RRM_1

Protein Analysis

206

Amino Acids

23.92

Weight (kDa)

9.48

Isoelectric Point (pI)

75.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 72 - 111 3.5e-09 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 468
AccB7I CCANNNNNTGG 1 cut(s) 455
AciI CCGC 1 cut(s) 592
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 3 cut(s) 72, 308, 336
AcyI GRCGYC 1 cut(s) 469
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 5 cut(s) 404, 455, 485, 557, 581
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 1 cut(s) 454
AluBI AGCT 3 cut(s) 7, 311, 320
AluI AGCT 3 cut(s) 7, 311, 320
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 2 cut(s) 4, 498
ApoI RAATTY 1 cut(s) 264
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
Asp700I GAANNNNTTC 1 cut(s) 321
AspLEI GCGC 3 cut(s) 80, 471, 543
AspS9I GGNCC 1 cut(s) 524
AsuHPI GGTGA 5 cut(s) 125, 371, 386, 437, 438
AvaII GGWCC 1 cut(s) 524
BaeI ACNNNNGTAYC 4 cut(s) 508, 541, 580, 613
BanI GGYRCC 1 cut(s) 468
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 16, 510
BccI CCATC 2 cut(s) 128, 135
BceAI ACGGC 1 cut(s) 598
BciT130I CCWGG 1 cut(s) 456
BfaI CTAG 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 472
BisI GCNGC 2 cut(s) 5, 499
BlsI GCNGC 2 cut(s) 6, 500
Bme1390I CCNGG 1 cut(s) 456
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 1 cut(s) 470
BmrFI CCNGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 37
BpiI GAAGAC 1 cut(s) 60
BsaHI GRCGYC 1 cut(s) 469
Bsc4I CCNNNNNNNGG 5 cut(s) 404, 455, 485, 557, 581
Bse1I ACTGG 2 cut(s) 153, 527
BseBI CCWGG 1 cut(s) 456
BseGI GGATG 2 cut(s) 43, 52
BseLI CCNNNNNNNGG 5 cut(s) 404, 455, 485, 557, 581
BseNI ACTGG 2 cut(s) 153, 527
BseXI GCAGC 2 cut(s) 16, 510
BsgI GTGCAG 1 cut(s) 308
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 468
BslI CCNNNNNNNGG 5 cut(s) 404, 455, 485, 557, 581
BsnI GGCC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 376, 443
BspACI CCGC 1 cut(s) 592
BspANI GGCC 1 cut(s) 163
BspHI TCATGA 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 470
BspQI GCTCTTC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 468
BsrI ACTGG 2 cut(s) 153, 527
BssMI GATC 2 cut(s) 376, 443
BssNI GRCGYC 1 cut(s) 469
Bst2UI CCWGG 1 cut(s) 456
Bst4CI ACNGT 3 cut(s) 416, 488, 524
Bst6I CTCTTC 1 cut(s) 327
BstACI GRCGYC 1 cut(s) 469
BstC8I GCNNGC 3 cut(s) 82, 467, 602
BstDEI CTNAG 1 cut(s) 101
BstF5I GGATG 2 cut(s) 43, 52
BstH2I RGCGCY 1 cut(s) 472
BstHHI GCGC 3 cut(s) 80, 471, 543
BstKTI GATC 2 cut(s) 379, 446
BstMBI GATC 2 cut(s) 376, 443
BstMWI GCNNNNNNNGC 5 cut(s) 56, 235, 317, 326, 591
BstNI CCWGG 1 cut(s) 456
BstSCI CCNGG 1 cut(s) 454
BstV1I GCAGC 2 cut(s) 16, 510
BstV2I GAAGAC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 134
BsuRI GGCC 1 cut(s) 163
BtsCI GGATG 2 cut(s) 43, 52
BtsIMutI CAGTG 2 cut(s) 146, 534
Cac8I GCNNGC 3 cut(s) 82, 467, 602
CciI TCATGA 1 cut(s) 130
CfoI GCGC 3 cut(s) 80, 471, 543
Cfr13I GGNCC 1 cut(s) 524
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 517
CviAII CATG 3 cut(s) 131, 257, 422
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 1 cut(s) 101
DinI GGCGCC 1 cut(s) 470
DpnI GATC 2 cut(s) 378, 445
DpnII GATC 2 cut(s) 376, 443
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 524
Eco57I CTGAAG 3 cut(s) 72, 308, 336
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 454
EgeI GGCGCC 1 cut(s) 470
EheI GGCGCC 1 cut(s) 470
FaeI CATG 3 cut(s) 134, 260, 425
FaiI YATR 8 cut(s) 11, 108, 132, 243, 258, 423, 510, 534
FatI CATG 3 cut(s) 130, 256, 421
Fnu4HI GCNGC 2 cut(s) 5, 499
FokI GGATG 2 cut(s) 50, 59
Fsp4HI GCNGC 2 cut(s) 5, 499
FspBI CTAG 1 cut(s) 399
GlaI GCGC 3 cut(s) 79, 470, 542
GluI GCNGC 2 cut(s) 5, 499
HaeII RGCGCY 1 cut(s) 472
HaeIII GGCC 1 cut(s) 163
HgaI GACGC 1 cut(s) 65
HhaI GCGC 3 cut(s) 80, 471, 543
Hin1I GRCGYC 1 cut(s) 469
Hin1II CATG 3 cut(s) 134, 260, 425
Hin6I GCGC 3 cut(s) 78, 469, 541
HinP1I GCGC 3 cut(s) 78, 469, 541
HinfI GANTC 2 cut(s) 547, 571
HphI GGTGA 5 cut(s) 125, 371, 386, 437, 438
Hpy166II GTNNAC 2 cut(s) 116, 202
Hpy188I TCNGA 2 cut(s) 263, 273
Hpy188III TCNNGA 6 cut(s) 131, 215, 367, 479, 551, 575
Hpy8I GTNNAC 2 cut(s) 116, 202
Hpy99I CGWCG 1 cut(s) 273
HpyAV CCTTC 3 cut(s) 241, 396, 555
HpyCH4III ACNGT 3 cut(s) 416, 488, 524
HpyCH4IV ACGT 2 cut(s) 118, 556
HpyCH4V TGCA 3 cut(s) 4, 281, 289
HpyF10VI GCNNNNNNNGC 5 cut(s) 56, 235, 317, 326, 591
HpyF3I CTNAG 1 cut(s) 101
HpySE526I ACGT 2 cut(s) 118, 556
Hsp92I GRCGYC 1 cut(s) 469
Hsp92II CATG 3 cut(s) 134, 260, 425
HspAI GCGC 3 cut(s) 78, 469, 541
KasI GGCGCC 1 cut(s) 468
Kzo9I GATC 2 cut(s) 376, 443
LguI GCTCTTC 1 cut(s) 327
Lsp1109I GCAGC 2 cut(s) 16, 510
LweI GCATC 1 cut(s) 37
MaeI CTAG 1 cut(s) 399
MaeII ACGT 2 cut(s) 118, 556
MaeIII GTNAC 1 cut(s) 410
MalI GATC 2 cut(s) 378, 445
MboI GATC 2 cut(s) 376, 443
MboII GAAGA 3 cut(s) 65, 314, 317
MluCI AATT 1 cut(s) 264
Mly113I GGCGCC 1 cut(s) 469
MlyI GAGTC 2 cut(s) 556, 580
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 7 cut(s) 28, 153, 333, 350, 365, 405, 504
MroXI GAANNNNTTC 1 cut(s) 321
MseI TTAA 1 cut(s) 344
MslI CAYNNNNRTG 2 cut(s) 111, 132
MspR9I CCNGG 1 cut(s) 456
MvaI CCWGG 1 cut(s) 456
MwoI GCNNNNNNNGC 5 cut(s) 56, 235, 317, 326, 591
NarI GGCGCC 1 cut(s) 469
NdeII GATC 2 cut(s) 376, 443
NlaIII CATG 3 cut(s) 134, 260, 425
NlaIV GGNNCC 1 cut(s) 470
PagI TCATGA 1 cut(s) 130
PciSI GCTCTTC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 321
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 2 cut(s) 6, 500
PleI GAGTC 2 cut(s) 555, 579
PluTI GGCGCC 1 cut(s) 472
PpsI GAGTC 2 cut(s) 555, 579
Psp6I CCWGG 1 cut(s) 454
PspGI CCWGG 1 cut(s) 454
PspN4I GGNNCC 1 cut(s) 470
PspPI GGNCC 1 cut(s) 524
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 2 cut(s) 111, 132
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 2 cut(s) 5, 499
Sau3AI GATC 2 cut(s) 376, 443
Sau96I GGNCC 1 cut(s) 524
SchI GAGTC 2 cut(s) 556, 580
ScrFI CCNGG 1 cut(s) 456
SfaNI GCATC 1 cut(s) 37
SfoI GGCGCC 1 cut(s) 470
SinI GGWCC 1 cut(s) 524
SmiMI CAYNNNNRTG 2 cut(s) 111, 132
Sse9I AATT 1 cut(s) 264
SsiI CCGC 1 cut(s) 592
SspDI GGCGCC 1 cut(s) 468
SspMI CTAG 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 454
TaaI ACNGT 3 cut(s) 416, 488, 524
TaiI ACGT 2 cut(s) 121, 559
TaqI TCGA 1 cut(s) 366
TasI AATT 1 cut(s) 264
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 2 cut(s) 153, 534
TseI GCWGC 2 cut(s) 4, 498
TspDTI ATGAA 2 cut(s) 54, 245
TspRI CASTG 2 cut(s) 153, 534
Van91I CCANNNNNTGG 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.