Rorug01G0278900

Arginine serine-rich-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008531
Physical Location & Seq
Forward (+)
39205257 .. 39205817
561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0278900.1

Sequence Viewer

Length: 561 bp
ATGAAGAGCTTCTTCTCAAAAGGGCCTATTGTAGCAATTCTGCTTTTGGCAATTTTCTTGGTTAGTGAAGCTCAGCAATGCCGTCCTAGTGGTAGAATCAGAGGAAGGAAGCCCCCTCCTGGACAATGCAACCAGGAGGACGACTCTGACTGCTGCAAAGCCGGAAAAATGTATCCAACATACACGTGCTCGCCACCTATGTCTGGCAACACCAAGGCCTACCTCACTCTTAACAGCTTCGAAGCAGGCGGTGACGGAGGAGGCCCATCTGAATGTGACGGCCAATACCATGATGACAACACTCCAGTTGTGGCACTATCCACCGGATGGTACAATGGTGGATCAAGGTGCCTTAAGAACATCAGAATTAACGGTAATGGGCAGAGTGTGGTGGCCATGGTGGTGGATGAGTGTGACTCTACTGAAGGATGTGATGCAGACCATGATTACCAGCCTCCTTGTCCTAACAACATTGTTGATGCCTCCAAGGCTGTTTGGAAAGCCTTGGGTGTGTCTGAAGACAATTGGGGCGGCTTAGATATCACTTGGTCCGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05168 map03040 map05168
Pfam Domains
Protein Families
RRM_1

Protein Analysis

186

Amino Acids

19.86

Weight (kDa)

4.64

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KWL1 PF24300 15 - 186 2.4e-68 Kiwellin-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 327
AciI CCGC 2 cut(s) 249, 531
AclWI GGATC 1 cut(s) 349
AcoI YGGCCR 2 cut(s) 280, 393
AcuI CTGAAG 2 cut(s) 444, 537
AcvI CACGTG 1 cut(s) 186
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 119, 203, 327
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 183
AjnI CCWGG 2 cut(s) 118, 132
AluBI AGCT 3 cut(s) 9, 71, 237
AluI AGCT 3 cut(s) 9, 71, 237
Alw21I GWGCWC 1 cut(s) 191
AlwI GGATC 1 cut(s) 349
AoxI GGCC 5 cut(s) 23, 216, 262, 280, 393
ApeKI GCWGC 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 3 cut(s) 23, 263, 549
AsuHPI GGTGA 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 1 cut(s) 549
BaeI ACNNNNGTAYC 2 cut(s) 322, 355
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 348
BbrPI CACGTG 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 1 cut(s) 140
BccI CCATC 2 cut(s) 274, 321
BceAI ACGGC 2 cut(s) 66, 295
BciT130I CCWGG 2 cut(s) 120, 134
BciVI GTATCC 1 cut(s) 183
BfaI CTAG 1 cut(s) 87
BfrI CTTAAG 1 cut(s) 353
BfuI GTATCC 1 cut(s) 183
BglI GCCNNNNNGGC 1 cut(s) 488
BisI GCNGC 2 cut(s) 154, 532
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 2 cut(s) 155, 533
Bme1390I CCNGG 2 cut(s) 120, 134
Bme18I GGWCC 1 cut(s) 549
BmgT120I GGNCC 3 cut(s) 23, 263, 549
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 2 cut(s) 120, 134
BmsI GCATC 3 cut(s) 424, 469, 544
BpiI GAAGAC 1 cut(s) 525
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 401, 433
BpmI CTGGAG 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 213, 396, 486, 504
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 203, 327
Bse1I ACTGG 1 cut(s) 305
Bse3DI GCAATG 1 cut(s) 83
BseBI CCWGG 2 cut(s) 120, 134
BseDI CCNNGG 4 cut(s) 213, 396, 486, 504
BseGI GGATG 3 cut(s) 332, 412, 434
BseLI CCNNNNNNNGG 3 cut(s) 119, 203, 327
BseMI GCAATG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 1 cut(s) 140
BshFI GGCC 5 cut(s) 25, 218, 264, 282, 395
BshNI GGYRCC 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 191
BsiSI CCGG 2 cut(s) 162, 324
BslI CCNNNNNNNGG 3 cut(s) 119, 203, 327
BsnI GGCC 5 cut(s) 25, 218, 264, 282, 395
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 1 cut(s) 341
Bsp1720I GCTNAGC 1 cut(s) 72
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 2 cut(s) 249, 531
BspANI GGCC 5 cut(s) 25, 218, 264, 282, 395
BspCNI CTCAG 1 cut(s) 85
BspLI GGNNCC 1 cut(s) 350
BspPI GGATC 1 cut(s) 349
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 348
BspTI CTTAAG 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 83
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 4 cut(s) 213, 396, 486, 504
BssMI GATC 1 cut(s) 341
BssT1I CCWWGG 4 cut(s) 213, 396, 486, 504
Bst2UI CCWGG 2 cut(s) 120, 134
Bst4CI ACNGT 1 cut(s) 374
BstAFI CTTAAG 1 cut(s) 353
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 191, 247
BstDEI CTNAG 3 cut(s) 72, 535, 558
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 3 cut(s) 332, 412, 434
BstKTI GATC 1 cut(s) 344
BstMBI GATC 1 cut(s) 341
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 2 cut(s) 120, 134
BstSCI CCNGG 2 cut(s) 118, 132
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 525
BstXI CCANNNNNNTGG 1 cut(s) 403
BsuI GTATCC 1 cut(s) 183
BsuRI GGCC 5 cut(s) 25, 218, 264, 282, 395
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 3 cut(s) 332, 412, 434
Cac8I GCNNGC 2 cut(s) 191, 247
Cfr13I GGNCC 3 cut(s) 23, 263, 549
Csp6I GTAC 1 cut(s) 331
CviAII CATG 3 cut(s) 290, 397, 443
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 3 cut(s) 72, 535, 558
DpnI GATC 1 cut(s) 343
DpnII GATC 1 cut(s) 341
EaeI YGGCCR 2 cut(s) 280, 393
Eco130I CCWWGG 4 cut(s) 213, 396, 486, 504
Eco147I AGGCCT 1 cut(s) 218
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 1 cut(s) 549
Eco57I CTGAAG 2 cut(s) 444, 537
Eco72I CACGTG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 23
EcoRII CCWGG 2 cut(s) 118, 132
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 4 cut(s) 213, 396, 486, 504
ErhI CCWWGG 4 cut(s) 213, 396, 486, 504
FaeI CATG 3 cut(s) 293, 400, 446
FaiI YATR 5 cut(s) 181, 200, 291, 398, 444
FalI AAGNNNNNCTT 1 cut(s) 28
FatI CATG 3 cut(s) 289, 396, 442
Fnu4HI GCNGC 2 cut(s) 154, 532
FokI GGATG 3 cut(s) 339, 419, 441
Fsp4HI GCNGC 2 cut(s) 154, 532
FspBI CTAG 1 cut(s) 87
GluI GCNGC 2 cut(s) 154, 532
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 5 cut(s) 25, 218, 264, 282, 395
HapII CCGG 2 cut(s) 162, 324
Hin1II CATG 3 cut(s) 293, 400, 446
HinfI GANTC 3 cut(s) 96, 143, 416
HpaII CCGG 2 cut(s) 162, 324
HphI GGTGA 1 cut(s) 263
Hpy188I TCNGA 6 cut(s) 101, 148, 271, 365, 517, 553
HpyAV CCTTC 2 cut(s) 99, 419
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 3 cut(s) 129, 156, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 3 cut(s) 72, 535, 558
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 3 cut(s) 293, 400, 446
Kzo9I GATC 1 cut(s) 341
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 3 cut(s) 424, 469, 544
MaeI CTAG 1 cut(s) 87
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 3 cut(s) 251, 275, 413
MalI GATC 1 cut(s) 343
MboI GATC 1 cut(s) 341
MboII GAAGA 3 cut(s) 4, 16, 530
MfeI CAATTG 1 cut(s) 523
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 4 cut(s) 36, 51, 366, 523
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 2 cut(s) 137, 410
MmeI TCCRAC 1 cut(s) 200
MnlI CCTC 8 cut(s) 95, 126, 130, 233, 251, 254, 465, 493
Mox20I TGGCCA 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 231, 354, 369
MslI CAYNNNNRTG 3 cut(s) 184, 271, 401
Msp20I TGGCCA 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 353
MspI CCGG 2 cut(s) 162, 324
MspR9I CCNGG 2 cut(s) 120, 134
MunI CAATTG 1 cut(s) 523
MvaI CCWGG 2 cut(s) 120, 134
MwoI GCNNNNNNNGC 1 cut(s) 488
NcoI CCATGG 1 cut(s) 396
NdeII GATC 1 cut(s) 341
NlaIII CATG 3 cut(s) 293, 400, 446
NlaIV GGNNCC 1 cut(s) 350
NmuCI GTSAC 3 cut(s) 251, 275, 413
NspV TTCGAA 1 cut(s) 240
PceI AGGCCT 1 cut(s) 218
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 96
PflMI CCANNNNNTGG 1 cut(s) 327
PfoI TCCNGGA 1 cut(s) 118
PkrI GCNGC 2 cut(s) 155, 533
PleI GAGTC 2 cut(s) 137, 410
PmaCI CACGTG 1 cut(s) 186
PmlI CACGTG 1 cut(s) 186
PpsI GAGTC 2 cut(s) 137, 410
Ppu21I YACGTR 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 118, 132
PspCI CACGTG 1 cut(s) 186
PspGI CCWGG 2 cut(s) 118, 132
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 3 cut(s) 23, 263, 549
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 3 cut(s) 184, 271, 401
SaqAI TTAA 3 cut(s) 231, 354, 369
SatI GCNGC 2 cut(s) 154, 532
Sau3AI GATC 1 cut(s) 341
Sau96I GGNCC 3 cut(s) 23, 263, 549
SchI GAGTC 2 cut(s) 137, 410
ScrFI CCNGG 2 cut(s) 120, 134
SduI GDGCHC 1 cut(s) 191
SetI ASST 7 cut(s) 11, 73, 188, 199, 225, 239, 350
SfaNI GCATC 3 cut(s) 424, 469, 544
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 1 cut(s) 549
SmiMI CAYNNNNRTG 3 cut(s) 184, 271, 401
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 4 cut(s) 36, 51, 366, 523
SseBI AGGCCT 1 cut(s) 218
SsiI CCGC 2 cut(s) 249, 531
SspMI CTAG 1 cut(s) 87
StuI AGGCCT 1 cut(s) 218
StyD4I CCNGG 2 cut(s) 118, 132
StyI CCWWGG 4 cut(s) 213, 396, 486, 504
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 36, 51, 366, 523
TauI GCSGC 1 cut(s) 534
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 3 cut(s) 231, 354, 369
Tru9I TTAA 3 cut(s) 231, 354, 369
TseFI GTSAC 3 cut(s) 251, 275, 413
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 3 cut(s) 251, 275, 413
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 270
Van91I CCANNNNNTGG 1 cut(s) 327
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.