MD07G1183400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003790
Physical Location & Seq
Reverse (-)
26337313 .. 26342587
5275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1183400.v1.1.491

Sequence Viewer

Length: 2673 bp
ATGAAGAAGCTCTTCTTTTTCAGATCTTCTGCTTCTAGTAATAACAACAATGTTCCTCCACCAACTGATAAGCAGATATCTGATGCCGAGAACTCCAATAAAAGTCCGGGTCTTAGAAGGAGCCGGTCATTGTCATCAGCAGCCTTTCTTGGAAGTGAGCCAACACAAACAGATTTTTCTTCTTCGAAATATCAAAGCAGATCTCCTTGTAGTAGTAGTGCAAGGAGCTTTCCTCATCAGCAATGTGACCAACCATCTTGTTGTCGAACTCTTACTCCTGAGAGGCTTAGGACTAAACATTTTGAAGTTCCGTCTGTCCAAAACGCACATGGATTGGAGAGGCCTGGTTCTGCTGGTTCTTCTAGAATGCGCCATGATTCGTCAGGGAGCTCTTCCACGTGCTCTAGTAATGTCTCAGCCAAAGTCTTGGACCGTTACATTGATGGAGAGCAGGAGGAACTGAGCAGACAAAAGAACAGTTCTTCCCAGAGACACTTGACTGGGAATGGTGGTGGTGGATGGCGTCCTCCGCGAACTCAGTTTACAGCACCCATTTCACCTAGGGCTCATTCATTTAGAGAGGCAAAAAGTTCTCGTCTTCGTTCATCATCCAAAGATGGGGCAGAAAATGGATTTGGGCATGAATCTCCCCGGAGGCTTGCAAAGAATGTCGTTGAGAGGCTCTCACAGTCTCATGTTATTCAACCAACACGTGAGAAGGAGTTTGACCATGATATTCCACTCACCATTGAGGATATTTATGGTAGACCAGATCTGATTGCCCAGAAAAATTATCCTGGAGATGACTACTCAAGCCTGCAGAAGTTGTTCTATAGTGAAAACTGTGGTGGTATAAACACCGATGAGACTGAAGAGGACATGGATGTAGAATTACAAAGAAGATTGAGGGAAGCAGAGGAGAAGGTCATGCTACTTTCTGACGAACTTGAACAGGAAAGCTTTCTTAGAGATAGTGGGTACAATGTTCCATCACTGATTCAGACAATTAGAAACCTAACTGAGCAGAGAGTAAGCATGGCACTTGAGGTTTCAAACCTCTTACAGTTGCGAATTGCTGAGAGGGCTTTTGCCAAGAAAGAACTCAGACTGGCAAACGGAGAACTGGAGTCGCGAACGAAAAAATTAGAGAAGGAGAAGAATGAGTTGCAGTCAGCATTGGAGAGGGAACTAGACAGAAGGTCGACTGACTGGTCAATGAAGCTTGAGAAGTATCAGTCAGAGGAGCAGAGGCTTCGCGAGCGAGTTAGAGAGCTTGCAGAGCAAAATGTCTCACTTCAAAGAGAAGTCTCTTCCTTTAATGTGAGGGAAACTGAGTATAGAAGTGTGGAAACAAAATCAGAGCAACAGCTTAACAGCCTTGCTACAAGGGTGGGGGAAATGAGAGAGGAGAATCAAGAACTGAAAAATAATCTCTCAGACTTGCAAGAGAAGTATAGAGCAGCAGAGGAAGACCGTGTTTGCATCCATAAAAATTTTGAAGAGAAGGATAAGGAGTGCAAGGATCTGCATAAGTCCATTACAAGATTACTGAGAACCTGCAAAGAACAAGAGAAGACAATAGATGGCTTGCGTGAAAGTTTTAGCGAGGAATTTAGGAAGAACCAATCCGTGGAGAGGTTTGATAAGCACGTCTCCAAATTGCAGATGGAGCAAATGAGGTTGACAGGAGTAGAATTGGCCTTGAGAAGAGAGTTGGAATCTCATAAGCTTGAAGTTGATTCTCTTCGGCATGAGAATATACACTTGTTAGACCGGTTGAGAGGAAGTGGGAAAGAAAATGGTGCTTTAACTTTCAAGCTAGATAAGGAAATGTGGGCACGAATCTCCTGCATGAAGAATCAAGGGCTATCGATTCTAAACGAGAGCTCCCAGTTATGTTCAAATTTACTGGAATTTGTTAAAGGGAAAGCAGGCCAACTTCCAGAAGCTAACCATGGCTTGGATGGGCAATTTTTTGTTGAATCTGAAATGAAAGTTCAGGGCATAAAGCGTGGAACTGAAAGCTTTGCAAGAAGTTTGCAGACAATGTCCGCTTTGCTGCATGAAAAATCCAGTCTGTCCAATTCGAAATTAGCATCCAAGTGCACGAATGCTGATGGACCAGTACATCCAGATGATCAGGCTCCAGAGGACGACATGAGATATGAGCTCAAAGCAGAAACTTTACTAACAAGTCTACTGAGAGAAAAGTTGTATTCAAAAGAGCTGGAGGTTGAGCAGTTGCAAGCTGAACTTGCAGCAGCTGTAAGAGGAAATGACATTCTCCAATGTGAAGTGCAAAATGCAATGGACAACCTTTCATGTCTCACCCACAAGTTGAAGGACCTTGAAATGCAGATGCTTAAGAAGGACGAGAACATAAACCAGCTTCAGAGTGACCTCCAGGCATCTACGAAAGAATTAACAGTTGCTCGGGGGATACTGCCTAAAATTTCAGAGGAGAGAGATATGATGTGGGAGGAGGTGAAGAAATACAACGAGAAGAACATGTTGCTGAACTCAGAGGTTAGCATGTTGAAGAAGAAGATCGAAACCCTTGACGAGGACATACTTCTAAAGGAAGGTCAGATCACAATTCTGAAAGATACCATAGGGAACAAGCCTTTCGACCTTCTGTCCAGTCCCGATAGGCGAGAATTTTTGCTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

891

Amino Acids

101.79

Weight (kDa)

6.11

Isoelectric Point (pI)

57.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7653 PF24670 567 - 691 1.4e-55 Domain of unknown function (DUF7653)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1210
Acc36I ACCTGC 1 cut(s) 1565
AccB7I CCANNNNNTGG 2 cut(s) 1630, 1960
AccI GTMKAC 3 cut(s) 766, 1202, 2197
AccII CGCG 3 cut(s) 532, 1132, 1257
AciI CCGC 2 cut(s) 530, 2052
AclWI GGATC 1 cut(s) 1530
AcsI RAATTY 6 cut(s) 1492, 1610, 1903, 1913, 2451, 2657
AcuI CTGAAG 2 cut(s) 891, 2375
AcvI CACGTG 2 cut(s) 399, 713
AcyI GRCGYC 1 cut(s) 523
AfaI GTAC 2 cut(s) 980, 2127
AfiI CCNNNNNNNGG 5 cut(s) 618, 1630, 1635, 1960, 2563
AflII CTTAAG 1 cut(s) 2363
AflIII ACRYGT 2 cut(s) 710, 2508
AgeI ACCGGT 1 cut(s) 1773
AhdI GACNNNNNGTC 2 cut(s) 1198, 2635
AjiI CACGTC 1 cut(s) 1651
AjnI CCWGG 3 cut(s) 343, 796, 2403
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AloI GAACNNNNNNTCC 4 cut(s) 259, 291, 467, 499
Alw21I GWGCWC 5 cut(s) 392, 404, 1889, 2108, 2172
Alw26I GTCTC 8 cut(s) 418, 484, 696, 860, 1294, 1312, 1657, 2330
Alw44I GTGCAC 1 cut(s) 2104
AlwI GGATC 1 cut(s) 1530
AlwNI CAGNNNCTG 1 cut(s) 2264
Ama87I CYCGRG 1 cut(s) 2433
AoxI GGCC 3 cut(s) 341, 1698, 1933
ApaLI GTGCAC 1 cut(s) 2104
ApeKI GCWGC 5 cut(s) 140, 1460, 2059, 2258, 2261
ApoI RAATTY 6 cut(s) 1492, 1610, 1903, 1913, 2451, 2657
AsiGI ACCGGT 1 cut(s) 1773
Asp700I GAANNNNTTC 3 cut(s) 11, 827, 960
AspA2I CCTAGG 1 cut(s) 560
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 430, 2120, 2344
AsuC2I CCSGG 2 cut(s) 108, 652
AsuHPI GGTGA 4 cut(s) 549, 736, 2320, 2497
AsuII TTCGAA 2 cut(s) 185, 2087
AvaI CYCGRG 1 cut(s) 2433
AvaII GGWCC 3 cut(s) 430, 2120, 2344
AvrII CCTAGG 1 cut(s) 560
BaeGI GKGCMC 2 cut(s) 1840, 2108
BanII GRGCYC 4 cut(s) 392, 568, 1889, 2172
BbrPI CACGTG 2 cut(s) 399, 713
BbsI GAAGAC 3 cut(s) 590, 1476, 1580
Bbv12I GWGCWC 5 cut(s) 392, 404, 1889, 2108, 2172
BbvI GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
BccI CCATC 9 cut(s) 262, 437, 513, 611, 997, 1577, 1660, 1958, 2111
BcgI CGANNNNNNTGC 4 cut(s) 1235, 1269, 1830, 1864
BciT130I CCWGG 3 cut(s) 345, 798, 2405
BciVI GTATCC 1 cut(s) 2433
BclI TGATCA 1 cut(s) 2137
BcnI CCSGG 2 cut(s) 108, 652
BcoDI GTCTC 8 cut(s) 418, 484, 696, 860, 1294, 1312, 1657, 2330
BfaI CTAG 6 cut(s) 36, 363, 405, 561, 1190, 1820
BfmI CTRYAG 2 cut(s) 818, 832
BfrI CTTAAG 1 cut(s) 2363
BfuAI ACCTGC 1 cut(s) 1565
BfuI GTATCC 1 cut(s) 2433
BglII AGATCT 3 cut(s) 23, 200, 772
BisI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
BlnI CCTAGG 1 cut(s) 560
BlsI GCNGC 5 cut(s) 142, 1462, 2061, 2260, 2263
Bme1390I CCNGG 5 cut(s) 108, 345, 652, 798, 2405
Bme18I GGWCC 3 cut(s) 430, 2120, 2344
BmeRI GACNNNNNGTC 2 cut(s) 1198, 2635
BmeT110I CYCGRG 1 cut(s) 2433
BmgBI CACGTC 1 cut(s) 1651
BmgT120I GGNCC 3 cut(s) 430, 2120, 2344
BmiI GGNNCC 2 cut(s) 122, 2145
BmrFI CCNGG 5 cut(s) 108, 345, 652, 798, 2405
BmrI ACTGGG 2 cut(s) 510, 1885
BmsI GCATC 5 cut(s) 73, 1491, 2105, 2349, 2417
BmuI ACTGGG 2 cut(s) 510, 1885
BpiI GAAGAC 3 cut(s) 590, 1476, 1580
BplI GAGNNNNNCTC 4 cut(s) 217, 249, 668, 700
BpmI CTGGAG 5 cut(s) 819, 1145, 2130, 2249, 2387
Bpu10I CCTNAGC 1 cut(s) 287
Bpu14I TTCGAA 2 cut(s) 185, 2087
BpuEI CTTGAG 4 cut(s) 796, 1064, 1244, 1723
BpuMI CCSGG 2 cut(s) 108, 652
Bsa29I ATCGAT 1 cut(s) 1871
BsaAI YACGTR 2 cut(s) 399, 713
BsaHI GRCGYC 1 cut(s) 523
BsaJI CCNNGG 4 cut(s) 560, 650, 1629, 1954
BsaWI WCCGGW 1 cut(s) 1773
BsaXI ACNNNNNCTCC 4 cut(s) 259, 289, 1871, 1901
Bsc4I CCNNNNNNNGG 5 cut(s) 618, 1630, 1635, 1960, 2563
Bse118I RCCGGY 2 cut(s) 123, 1773
Bse1I ACTGG 9 cut(s) 505, 1113, 1128, 1214, 1891, 1914, 2073, 2123, 2640
Bse3DI GCAATG 2 cut(s) 248, 2313
BseBI CCWGG 3 cut(s) 345, 798, 2405
BseCI ATCGAT 1 cut(s) 1871
BseDI CCNNGG 4 cut(s) 560, 650, 1629, 1954
BseGI GGATG 7 cut(s) 524, 608, 889, 1482, 1969, 2096, 2128
BseLI CCNNNNNNNGG 5 cut(s) 618, 1630, 1635, 1960, 2563
BseMI GCAATG 2 cut(s) 248, 2313
BseNI ACTGG 9 cut(s) 505, 1113, 1128, 1214, 1891, 1914, 2073, 2123, 2640
BseRI GAGGAG 5 cut(s) 932, 1256, 1421, 2474, 2495
BseSI GKGCMC 2 cut(s) 1840, 2108
BseXI GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
Bsh1236I CGCG 3 cut(s) 532, 1132, 1257
BshFI GGCC 3 cut(s) 343, 1700, 1935
BshTI ACCGGT 1 cut(s) 1773
BshVI ATCGAT 1 cut(s) 1871
BsiHKAI GWGCWC 5 cut(s) 392, 404, 1889, 2108, 2172
BsiHKCI CYCGRG 1 cut(s) 2433
BsiSI CCGG 4 cut(s) 107, 124, 652, 1774
BslFI GGGAC 1 cut(s) 2628
BslI CCNNNNNNNGG 5 cut(s) 618, 1630, 1635, 1960, 2563
BsmAI GTCTC 8 cut(s) 418, 484, 696, 860, 1294, 1312, 1657, 2330
BsmBI CGTCTC 1 cut(s) 1657
BsmFI GGGAC 1 cut(s) 2628
BsmI GAATGC 2 cut(s) 372, 2116
BsnI GGCC 3 cut(s) 343, 1700, 1935
BsoBI CYCGRG 1 cut(s) 2433
Bsp119I TTCGAA 2 cut(s) 185, 2087
Bsp1286I GDGCHC 7 cut(s) 392, 404, 568, 1840, 1889, 2108, 2172
Bsp143I GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
Bsp19I CCATGG 1 cut(s) 1954
Bsp68I TCGCGA 2 cut(s) 1132, 1257
BspACI CCGC 2 cut(s) 530, 2052
BspANI GGCC 3 cut(s) 343, 1700, 1935
BspDI ATCGAT 1 cut(s) 1871
BspFNI CGCG 3 cut(s) 532, 1132, 1257
BspLI GGNNCC 2 cut(s) 122, 2145
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 1 cut(s) 1565
BspPI GGATC 1 cut(s) 1530
BspQI GCTCTTC 2 cut(s) 17, 397
BspT104I TTCGAA 2 cut(s) 185, 2087
BspTI CTTAAG 1 cut(s) 2363
BsrDI GCAATG 2 cut(s) 248, 2313
BsrFI RCCGGY 2 cut(s) 123, 1773
BsrI ACTGG 9 cut(s) 505, 1113, 1128, 1214, 1891, 1914, 2073, 2123, 2640
BssAI RCCGGY 2 cut(s) 123, 1773
BssECI CCNNGG 4 cut(s) 560, 650, 1629, 1954
BssMI GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
BssNI GRCGYC 1 cut(s) 523
BssT1I CCWWGG 2 cut(s) 560, 1954
Bst2UI CCWGG 3 cut(s) 345, 798, 2405
Bst4CI ACNGT 7 cut(s) 434, 479, 690, 845, 1065, 1475, 2428
Bst6I CTCTTC 7 cut(s) 17, 397, 867, 1315, 1494, 1702, 1749
BstACI GRCGYC 1 cut(s) 523
BstAFI CTTAAG 1 cut(s) 2363
BstBAI YACGTR 2 cut(s) 399, 713
BstBI TTCGAA 2 cut(s) 185, 2087
BstC8I GCNNGC 7 cut(s) 660, 818, 1259, 1275, 1589, 1933, 2247
BstDSI CCRYGG 2 cut(s) 1629, 1954
BstENI CCTNNNNNAGG 1 cut(s) 2561
BstF5I GGATG 7 cut(s) 524, 608, 889, 1482, 1969, 2096, 2128
BstFNI CGCG 3 cut(s) 532, 1132, 1257
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 7 cut(s) 26, 203, 775, 1525, 2140, 2550, 2592
BstMAI GTCTC 8 cut(s) 418, 484, 696, 860, 1294, 1312, 1657, 2330
BstMBI GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
BstMWI GCNNNNNNNGC 6 cut(s) 529, 1082, 1258, 1279, 1669, 2255
BstNI CCWGG 3 cut(s) 345, 798, 2405
BstNSI RCATGY 2 cut(s) 2512, 2536
BstSCI CCNGG 5 cut(s) 106, 343, 650, 796, 2403
BstSFI CTRYAG 2 cut(s) 818, 832
BstSLI GKGCMC 2 cut(s) 1840, 2108
BstUI CGCG 3 cut(s) 532, 1132, 1257
BstV1I GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
BstV2I GAAGAC 3 cut(s) 590, 1476, 1580
BstX2I RGATCY 4 cut(s) 23, 200, 772, 1522
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 4 cut(s) 23, 200, 772, 1522
Bsu15I ATCGAT 1 cut(s) 1871
BsuI GTATCC 1 cut(s) 2433
BsuRI GGCC 3 cut(s) 343, 1700, 1935
BsuTUI ATCGAT 1 cut(s) 1871
BtgI CCRYGG 2 cut(s) 1629, 1954
BtrI CACGTC 1 cut(s) 1651
BtsCI GGATG 7 cut(s) 524, 608, 889, 1482, 1969, 2096, 2128
BtsIMutI CAGTG 1 cut(s) 992
BtuMI TCGCGA 2 cut(s) 1132, 1257
BveI ACCTGC 1 cut(s) 1565
Cac8I GCNNGC 7 cut(s) 660, 818, 1259, 1275, 1589, 1933, 2247
CaiI CAGNNNCTG 1 cut(s) 2264
CfoI GCGC 1 cut(s) 372
Cfr10I RCCGGY 2 cut(s) 123, 1773
Cfr13I GGNCC 3 cut(s) 430, 2120, 2344
ClaI ATCGAT 1 cut(s) 1871
CseI GACGC 1 cut(s) 512
Csp6I GTAC 2 cut(s) 979, 2126
CspAI ACCGGT 1 cut(s) 1773
CviQI GTAC 2 cut(s) 979, 2126
DpnI GATC 7 cut(s) 25, 202, 774, 1524, 2139, 2549, 2591
DpnII GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
DrdI GACNNNNNNGTC 1 cut(s) 1210
DriI GACNNNNNGTC 2 cut(s) 1198, 2635
DseDI GACNNNNNNGTC 1 cut(s) 1210
Eam1104I CTCTTC 7 cut(s) 17, 397, 867, 1315, 1494, 1702, 1749
Eam1105I GACNNNNNGTC 2 cut(s) 1198, 2635
EarI CTCTTC 7 cut(s) 17, 397, 867, 1315, 1494, 1702, 1749
Ecl136II GAGCTC 3 cut(s) 390, 1887, 2170
Eco130I CCWWGG 2 cut(s) 560, 1954
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 4 cut(s) 392, 568, 1889, 2172
Eco32I GATATC 1 cut(s) 78
Eco47I GGWCC 3 cut(s) 430, 2120, 2344
Eco53kI GAGCTC 3 cut(s) 390, 1887, 2170
Eco57I CTGAAG 2 cut(s) 891, 2375
Eco72I CACGTG 2 cut(s) 399, 713
Eco88I CYCGRG 1 cut(s) 2433
EcoICRI GAGCTC 3 cut(s) 390, 1887, 2170
EcoNI CCTNNNNNAGG 1 cut(s) 2561
EcoO109I RGGNCCY 1 cut(s) 2344
EcoRII CCWGG 3 cut(s) 343, 796, 2403
EcoRV GATATC 1 cut(s) 78
EcoT14I CCWWGG 2 cut(s) 560, 1954
EcoT38I GRGCYC 4 cut(s) 392, 568, 1889, 2172
ErhI CCWWGG 2 cut(s) 560, 1954
Esp3I CGTCTC 1 cut(s) 1657
FalI AAGNNNNNCTT 4 cut(s) 28, 31, 2238, 2270
FaqI GGGAC 1 cut(s) 2628
FbaI TGATCA 1 cut(s) 2137
FblI GTMKAC 3 cut(s) 766, 1202, 2197
Fnu4HI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
FokI GGATG 7 cut(s) 531, 595, 896, 1469, 1976, 2083, 2115
FriOI GRGCYC 4 cut(s) 392, 568, 1889, 2172
Fsp4HI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
FspBI CTAG 6 cut(s) 36, 363, 405, 561, 1190, 1820
GlaI GCGC 1 cut(s) 371
GluI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
GsuI CTGGAG 5 cut(s) 819, 1145, 2130, 2249, 2387
HaeIII GGCC 3 cut(s) 343, 1700, 1935
HapII CCGG 4 cut(s) 107, 124, 652, 1774
HgaI GACGC 1 cut(s) 512
HhaI GCGC 1 cut(s) 372
Hin1I GRCGYC 1 cut(s) 523
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 2 cut(s) 1203, 1683
HindII GTYRAC 2 cut(s) 1203, 1683
HindIII AAGCTT 4 cut(s) 958, 1220, 1727, 2023
HpaII CCGG 4 cut(s) 107, 124, 652, 1774
HphI GGTGA 4 cut(s) 549, 736, 2320, 2497
Hpy166II GTNNAC 6 cut(s) 543, 767, 1203, 1683, 2106, 2198
Hpy188III TCNNGA 9 cut(s) 278, 363, 1131, 1256, 1415, 1943, 2132, 2147, 2645
Hpy8I GTNNAC 6 cut(s) 543, 767, 1203, 1683, 2106, 2198
HpyCH4III ACNGT 7 cut(s) 434, 479, 690, 845, 1065, 1475, 2428
HpyCH4IV ACGT 3 cut(s) 398, 712, 1650
HpyF10VI GCNNNNNNNGC 6 cut(s) 529, 1082, 1258, 1279, 1669, 2255
HpySE526I ACGT 3 cut(s) 398, 712, 1650
Hsp92I GRCGYC 1 cut(s) 523
HspAI GCGC 1 cut(s) 370
Ksp22I TGATCA 1 cut(s) 2137
Kzo9I GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
LguI GCTCTTC 2 cut(s) 17, 397
LmnI GCTCC 7 cut(s) 120, 225, 387, 1243, 1669, 1892, 2149
Lsp1109I GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
LweI GCATC 5 cut(s) 73, 1491, 2105, 2349, 2417
MaeI CTAG 6 cut(s) 36, 363, 405, 561, 1190, 1820
MaeII ACGT 3 cut(s) 398, 712, 1650
MaeIII GTNAC 3 cut(s) 245, 434, 2396
MalI GATC 7 cut(s) 25, 202, 774, 1524, 2139, 2549, 2591
MboI GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
MflI RGATCY 4 cut(s) 23, 200, 772, 1522
MhlI GDGCHC 7 cut(s) 392, 404, 568, 1840, 1889, 2108, 2172
MlyI GAGTC 1 cut(s) 1136
MmeI TCCRAC 1 cut(s) 1695
MroXI GAANNNNTTC 3 cut(s) 11, 827, 960
MseI TTAA 6 cut(s) 1317, 1371, 1808, 1920, 2364, 2423
MslI CAYNNNNRTG 2 cut(s) 2101, 2133
MspA1I CMGCKG 1 cut(s) 2264
MspCI CTTAAG 1 cut(s) 2363
MspI CCGG 4 cut(s) 107, 124, 652, 1774
MspR9I CCNGG 5 cut(s) 108, 345, 652, 798, 2405
Mva1269I GAATGC 2 cut(s) 372, 2116
MvaI CCWGG 3 cut(s) 345, 798, 2405
MvnI CGCG 3 cut(s) 532, 1132, 1257
MwoI GCNNNNNNNGC 6 cut(s) 529, 1082, 1258, 1279, 1669, 2255
NciI CCSGG 2 cut(s) 108, 652
NcoI CCATGG 1 cut(s) 1954
NdeII GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
NlaIV GGNNCC 2 cut(s) 122, 2145
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 245, 2396
NruI TCGCGA 2 cut(s) 1132, 1257
NspI RCATGY 2 cut(s) 2512, 2536
NspV TTCGAA 2 cut(s) 185, 2087
PceI AGGCCT 1 cut(s) 343
PciI ACATGT 1 cut(s) 2508
PciSI GCTCTTC 2 cut(s) 17, 397
PctI GAATGC 2 cut(s) 372, 2116
PdmI GAANNNNTTC 3 cut(s) 11, 827, 960
PflFI GACNNNGTC 1 cut(s) 2047
PflMI CCANNNNNTGG 2 cut(s) 1630, 1960
PfoI TCCNGGA 1 cut(s) 796
PinAI ACCGGT 1 cut(s) 1773
PkrI GCNGC 5 cut(s) 142, 1462, 2061, 2260, 2263
PleI GAGTC 1 cut(s) 1135
PmaCI CACGTG 2 cut(s) 399, 713
PmlI CACGTG 2 cut(s) 399, 713
PpsI GAGTC 1 cut(s) 1135
Ppu21I YACGTR 2 cut(s) 399, 713
PpuMI RGGWCCY 1 cut(s) 2344
PscI ACATGT 1 cut(s) 2508
Psp124BI GAGCTC 3 cut(s) 392, 1889, 2172
Psp5II RGGWCCY 1 cut(s) 2344
Psp6I CCWGG 3 cut(s) 343, 796, 2403
PspCI CACGTG 2 cut(s) 399, 713
PspGI CCWGG 3 cut(s) 343, 796, 2403
PspN4I GGNNCC 2 cut(s) 122, 2145
PspPI GGNCC 3 cut(s) 430, 2120, 2344
PspPPI RGGWCCY 1 cut(s) 2344
PsrI GAACNNNNNNTAC 2 cut(s) 526, 558
PstI CTGCAG 1 cut(s) 822
PstNI CAGNNNCTG 1 cut(s) 2264
PsuI RGATCY 4 cut(s) 23, 200, 772, 1522
PsyI GACNNNGTC 1 cut(s) 2047
PvuII CAGCTG 1 cut(s) 2264
RruI TCGCGA 2 cut(s) 1132, 1257
RsaI GTAC 2 cut(s) 980, 2127
RsaNI GTAC 2 cut(s) 979, 2126
RseI CAYNNNNRTG 2 cut(s) 2101, 2133
SacI GAGCTC 3 cut(s) 392, 1889, 2172
SalI GTCGAC 1 cut(s) 1201
SapI GCTCTTC 2 cut(s) 17, 397
SaqAI TTAA 6 cut(s) 1317, 1371, 1808, 1920, 2364, 2423
SatI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
Sau3AI GATC 7 cut(s) 23, 200, 772, 1522, 2137, 2547, 2589
Sau96I GGNCC 3 cut(s) 430, 2120, 2344
SchI GAGTC 1 cut(s) 1136
ScrFI CCNGG 5 cut(s) 108, 345, 652, 798, 2405
SduI GDGCHC 7 cut(s) 392, 404, 568, 1840, 1889, 2108, 2172
SfaNI GCATC 5 cut(s) 73, 1491, 2105, 2349, 2417
SfcI CTRYAG 2 cut(s) 818, 832
SfuI TTCGAA 2 cut(s) 185, 2087
SinI GGWCC 3 cut(s) 430, 2120, 2344
SmiMI CAYNNNNRTG 2 cut(s) 2101, 2133
SmlI CTYRAG 5 cut(s) 811, 1043, 1223, 1702, 2363
SmoI CTYRAG 5 cut(s) 811, 1043, 1223, 1702, 2363
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 2 cut(s) 530, 2052
SspMI CTAG 6 cut(s) 36, 363, 405, 561, 1190, 1820
SstI GAGCTC 3 cut(s) 392, 1889, 2172
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 5 cut(s) 106, 343, 650, 796, 2403
StyI CCWWGG 2 cut(s) 560, 1954
TaaI ACNGT 7 cut(s) 434, 479, 690, 845, 1065, 1475, 2428
TaiI ACGT 3 cut(s) 401, 715, 1653
TaqI TCGA 7 cut(s) 185, 265, 1202, 1871, 2087, 2550, 2628
TatI WGTACW 1 cut(s) 2125
Tru1I TTAA 6 cut(s) 1317, 1371, 1808, 1920, 2364, 2423
Tru9I TTAA 6 cut(s) 1317, 1371, 1808, 1920, 2364, 2423
TscAI CASTG 1 cut(s) 999
TseFI GTSAC 2 cut(s) 245, 2396
TseI GCWGC 5 cut(s) 140, 1460, 2059, 2258, 2261
Tsp45I GTSAC 2 cut(s) 245, 2396
TspGWI ACGGA 3 cut(s) 300, 1131, 1618
TspRI CASTG 1 cut(s) 999
Tth111I GACNNNGTC 1 cut(s) 2047
Van91I CCANNNNNTGG 2 cut(s) 1630, 1960
Vha464I CTTAAG 1 cut(s) 2363
VneI GTGCAC 1 cut(s) 2104
VpaK11BI GGWCC 3 cut(s) 430, 2120, 2344
XagI CCTNNNNNAGG 1 cut(s) 2561
XapI RAATTY 6 cut(s) 1492, 1610, 1903, 1913, 2451, 2657
XbaI TCTAGA 1 cut(s) 362
XceI RCATGY 2 cut(s) 2512, 2536
XcmI CCANNNNNNNNNTGG 3 cut(s) 326, 1663, 1961
XmaJI CCTAGG 1 cut(s) 560
XmiI GTMKAC 3 cut(s) 766, 1202, 2197
XmnI GAANNNNTTC 3 cut(s) 11, 827, 960
XspI CTAG 6 cut(s) 36, 363, 405, 561, 1190, 1820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.