pycom07g17600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003790
Physical Location & Seq
Reverse (-)
20105225 .. 20108349
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17600.1

Sequence Viewer

Length: 2673 bp
ATGAAGAAGCTCTTCTTTTTCAGATCTTCTGCTTCTAGTAATAACAACAATGTTCCTCCACCAACTGATAAGCAGATATCTGATGCCGAGAACTCCAATAAAAGTCCGGCTCTTAGAAGGAGCCGGTCATTGTCATCAGCAGCATTTCTTGGAAGTGAGCCAACGCAAACAGATTTTTCTTCTTCGAAATATCAAAGCAGATCTCCTTGTAGTAGTAGTGCAAGGAGCTTTCCTCATCAGCAATGCGACCAACCATCTTGTTGTCGAACTCTTACTCCTGAGAGGCATAGGACTAAACATTTTGAAGTTCCATCTCTCCAAAACACACATGGATTGGAGAGGCCTGGTTCTGCTGGTTCTTCTAGAATGCGCCATGATTCGTCAGGGAGCTCTTCCACTTGCTCTAGTAATGTATCAGCCAAAGTCTTGGACCGTTACATTGACGGAGAGCAGGAGGAACTGAGCAGACAAAAGAACAGTTCTTCCCAGAGACACTTGACTGGGAATGGTGGTGGTGGATGGCGTCCACCGCGAACTCAGTTTACAGCACCCATTTCACCTAGGGCTCATTCATATAGAGAGACAAAAAGTTCTCGTCTTCGTTCATCATCCAAAGACGGGGCAGAAAATGGATTTGGGCATGAATCTCCCCGGAGGCTTGCAAAGAATGTCGTTGAGAGGCTCTCACAGTCTCACGTTATTCAACCAACACGTGAGAAGGAGTTTGACCATGATATTCCACTCACCATTGAGGATATTTATGGTAGATCAGATCTGATTGCCCAGAAAAATTATCCTGGAGATGACTACTCAAGCCTGCAGAAGTTGTTCTATGGTGAAAACTGTGATGGTATAAACACCGATGAGACTGAAGAGGACATGGATGTAGAATTACAAAGAAGATTGAGGGAAGCAGAGGAGAAGGTCATGCTACTTTCTGACGAACTTGAACAGGAAAGCTTTCTTAGAGATAGTGGGTACAATGTTCCATCACTGATTCAGACAATTAGAAACCTAACTGAGCAGAGGGTAAGCTTGGCACTTGAGGTTTCAAACCTCTTACAGTTGCGAATTGCTGAGAGGGCTTCTGCCAAGAAAGAACTCAGACTGGCAAACGGAGAACTGGAGTCGCGAACGAAAAAATTAGAGAAGGAGAAGAATGAGTTGCAGTCAGCATTGGAGAGGGAACTAGACAGAAGGTCGACTGACTGGTCACTTAAGCTTGAGAAGTATCAGTCAGAGGAGCAGAGGCTTCGTGAGCGAGTTAGAGAGCTTGCAGAGCAAAATGTCTCACTTCAAAGAGAAGTCTCTTCCTTTAATGTGAGGGAAACTGAGTATAGAAGTGTGGAAACAAAATCAGAGCAACAGCTTAACAGCCTTGTTACAAGGGTGGGGGAAATGAGAGAGGAGAATCAAGAACTGAAAAATAATCTCTCAGACTTGCAAGAGAAGTATAGAGCAGCAGAGGAAGACCGTGTTTGCATCCATAAAAATTTTGATGAGAAGGATAAGGAGTGCAAGGATCTGCATAAGTCCATTACAAGATTACTGAGAACCTGCAAAGAACAAGAGAAGACAATAGATGGCTTGCGTGAAAGTTTTAGCGAGGAATTTAGGAAGAACCAATCCGTGGAGAGGTTTGATAAGCACGTCACCAAATTGCAGATGGAGCAAATGAGGTTGACAGGAGTAGAACTGGCCTTGAGAAGGGAGTTGGAATCTCATAAGCTTGAAGTTGATTCTCTTCGGCATGAGAATATACACTTGTTAGACCGGTTGAGAGGAAGTGGGAAAGAAAATGGTGCCTTAACTTTCAAGCTAGATAAGGAAATGTCGGCACGAGTCTCCTACATGAAGAATCAAGGGCTATCAATTCTAAACGAGAGCTCCCAGTTATGTTCAAATTTACTAGAATTTGTTAAAGGGAAAGCAGGCCAACTTCCAGAAGCTAACCATGGCTTGGATGGGCAATTTTTTGTTGAATCTGAAATGAAAGTTCAGGGCATAAAGCGTGGAACTGAAAGCTTTGCAAGAAGTTTGCAGACAATGTCCACTTTGCTGCATGAAAAATCCAGTCTGTCCACTTCAAAATTAGCATCTAAGTGCACGAATGCTGATGGACCAGCACATCCAGATGATCAGGCTCCAGAGGATGACATGAGATATGAGCTCAAAGCAGAAACTTTACTAACAAGTCTACTGAGAGAAAAGTTGTATTCAAAAGAGCTGGAAGTTGAGCAGTTGCAAGCCGAACTTGCAGCAGCTGTAAGAGGAAATGACATTCTCCGATGTGAAGTGCAAAATGCAATGGACAACCTTTCATGTCTCACCCACAAGTTGAAGGACCTTGAAATGCAGATGCTTAAGAAGGACGAGAACATAAACCAGCTTCAGAGTGACCTCCAGGCGTCAACGAAAGAATTAACAGTTACTCGGGGGATACTGCCTAAAATTTCAGAGGAGAGAGACATGATGTGGGAGGAGGTGAAGAAATACAACGAGAAGAACATGTTGCTGAACTCAGAGGTTAACATGTTGAAGAAGAAGATCGAAACCCTTGACGAGGACATACTTCTAAAGGAAGGTCAGATCACAATTCTGAAAGATACCATAGGGAACAAGCCTTTCGACCTTCTGTCCAGTCCCGATAGGCGAGAATTTTTGCTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

891

Amino Acids

101.89

Weight (kDa)

6.13

Isoelectric Point (pI)

57.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7653 PF24670 567 - 691 4.3e-54 Domain of unknown function (DUF7653)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1210
Acc36I ACCTGC 1 cut(s) 1565
AccB1I GGYRCC 1 cut(s) 1802
AccB7I CCANNNNNTGG 2 cut(s) 1630, 1960
AccI GTMKAC 2 cut(s) 1202, 2197
AccII CGCG 2 cut(s) 532, 1132
AciI CCGC 1 cut(s) 530
AclWI GGATC 1 cut(s) 1530
AcsI RAATTY 6 cut(s) 1492, 1610, 1903, 1913, 2451, 2657
AcuI CTGAAG 2 cut(s) 891, 2375
AcvI CACGTG 1 cut(s) 713
AcyI GRCGYC 2 cut(s) 523, 2408
AfaI GTAC 1 cut(s) 980
AfiI CCNNNNNNNGG 6 cut(s) 618, 1630, 1635, 1707, 1960, 2563
AflII CTTAAG 2 cut(s) 1217, 2363
AflIII ACRYGT 3 cut(s) 710, 2508, 2532
AgeI ACCGGT 1 cut(s) 1773
AhdI GACNNNNNGTC 2 cut(s) 1198, 2635
AjiI CACGTC 1 cut(s) 1651
AjnI CCWGG 3 cut(s) 343, 796, 2403
AjuI GAANNNNNNNTTGG 2 cut(s) 618, 650
AloI GAACNNNNNNTCC 4 cut(s) 259, 291, 467, 499
Alw21I GWGCWC 4 cut(s) 392, 1889, 2108, 2172
Alw26I GTCTC 9 cut(s) 484, 575, 696, 860, 1294, 1312, 1849, 2330, 2460
Alw44I GTGCAC 1 cut(s) 2104
AlwI GGATC 1 cut(s) 1530
AlwNI CAGNNNCTG 1 cut(s) 2264
Ama87I CYCGRG 1 cut(s) 2433
AoxI GGCC 3 cut(s) 341, 1698, 1933
ApaLI GTGCAC 1 cut(s) 2104
ApeKI GCWGC 5 cut(s) 140, 1460, 2059, 2258, 2261
ApoI RAATTY 6 cut(s) 1492, 1610, 1903, 1913, 2451, 2657
AsiGI ACCGGT 1 cut(s) 1773
Asp700I GAANNNNTTC 3 cut(s) 11, 827, 960
AspA2I CCTAGG 1 cut(s) 560
AspLEI GCGC 1 cut(s) 372
AspS9I GGNCC 3 cut(s) 430, 2120, 2344
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 6 cut(s) 549, 736, 848, 1645, 2320, 2497
AsuII TTCGAA 1 cut(s) 185
AvaI CYCGRG 1 cut(s) 2433
AvaII GGWCC 3 cut(s) 430, 2120, 2344
AvrII CCTAGG 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 2108
BanI GGYRCC 1 cut(s) 1802
BanII GRGCYC 4 cut(s) 392, 568, 1889, 2172
BauI CACGAG 1 cut(s) 1839
BbrPI CACGTG 1 cut(s) 713
BbsI GAAGAC 3 cut(s) 590, 1476, 1580
Bbv12I GWGCWC 4 cut(s) 392, 1889, 2108, 2172
BbvI GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
BccI CCATC 9 cut(s) 262, 319, 513, 842, 997, 1577, 1660, 1958, 2111
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BciT130I CCWGG 3 cut(s) 345, 798, 2405
BciVI GTATCC 1 cut(s) 2433
BclI TGATCA 1 cut(s) 2137
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 9 cut(s) 484, 575, 696, 860, 1294, 1312, 1849, 2330, 2460
BfaI CTAG 7 cut(s) 36, 363, 405, 561, 1190, 1820, 1910
BfmI CTRYAG 1 cut(s) 818
BfrI CTTAAG 2 cut(s) 1217, 2363
BfuAI ACCTGC 1 cut(s) 1565
BfuI GTATCC 1 cut(s) 2433
BglII AGATCT 3 cut(s) 23, 200, 772
BisI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
BlnI CCTAGG 1 cut(s) 560
BlsI GCNGC 5 cut(s) 142, 1462, 2061, 2260, 2263
Bme1390I CCNGG 4 cut(s) 345, 652, 798, 2405
Bme18I GGWCC 3 cut(s) 430, 2120, 2344
BmeRI GACNNNNNGTC 2 cut(s) 1198, 2635
BmeT110I CYCGRG 1 cut(s) 2433
BmgBI CACGTC 1 cut(s) 1651
BmgT120I GGNCC 3 cut(s) 430, 2120, 2344
BmiI GGNNCC 3 cut(s) 122, 1804, 2145
BmrFI CCNGG 4 cut(s) 345, 652, 798, 2405
BmrI ACTGGG 2 cut(s) 510, 1885
BmsI GCATC 4 cut(s) 73, 1491, 2105, 2349
BmuI ACTGGG 2 cut(s) 510, 1885
BpiI GAAGAC 3 cut(s) 590, 1476, 1580
BplI GAGNNNNNCTC 4 cut(s) 217, 249, 668, 700
BpmI CTGGAG 4 cut(s) 819, 1145, 2130, 2387
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 4 cut(s) 796, 1064, 1244, 1723
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 713
BsaHI GRCGYC 2 cut(s) 523, 2408
BsaJI CCNNGG 4 cut(s) 560, 650, 1629, 1954
BsaWI WCCGGW 1 cut(s) 1773
BsaXI ACNNNNNCTCC 4 cut(s) 259, 289, 1871, 1901
Bsc4I CCNNNNNNNGG 6 cut(s) 618, 1630, 1635, 1707, 1960, 2563
Bse118I RCCGGY 2 cut(s) 123, 1773
Bse1I ACTGG 8 cut(s) 505, 1113, 1128, 1214, 1701, 1891, 2073, 2640
Bse3DI GCAATG 2 cut(s) 248, 2313
BseBI CCWGG 3 cut(s) 345, 798, 2405
BseDI CCNNGG 4 cut(s) 560, 650, 1629, 1954
BseGI GGATG 7 cut(s) 524, 608, 889, 1482, 1969, 2128, 2158
BseLI CCNNNNNNNGG 6 cut(s) 618, 1630, 1635, 1707, 1960, 2563
BseMI GCAATG 2 cut(s) 248, 2313
BseNI ACTGG 8 cut(s) 505, 1113, 1128, 1214, 1701, 1891, 2073, 2640
BseRI GAGGAG 5 cut(s) 932, 1256, 1421, 2474, 2495
BseSI GKGCMC 1 cut(s) 2108
BseXI GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
Bsh1236I CGCG 2 cut(s) 532, 1132
BshFI GGCC 3 cut(s) 343, 1700, 1935
BshNI GGYRCC 1 cut(s) 1802
BshTI ACCGGT 1 cut(s) 1773
BsiHKAI GWGCWC 4 cut(s) 392, 1889, 2108, 2172
BsiHKCI CYCGRG 1 cut(s) 2433
BsiSI CCGG 4 cut(s) 107, 124, 652, 1774
BslFI GGGAC 1 cut(s) 2628
BslI CCNNNNNNNGG 6 cut(s) 618, 1630, 1635, 1707, 1960, 2563
BsmAI GTCTC 9 cut(s) 484, 575, 696, 860, 1294, 1312, 1849, 2330, 2460
BsmFI GGGAC 1 cut(s) 2628
BsmI GAATGC 2 cut(s) 372, 2116
BsnI GGCC 3 cut(s) 343, 1700, 1935
BsoBI CYCGRG 1 cut(s) 2433
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 5 cut(s) 392, 568, 1889, 2108, 2172
Bsp143I GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
Bsp19I CCATGG 1 cut(s) 1954
Bsp68I TCGCGA 1 cut(s) 1132
BspACI CCGC 1 cut(s) 530
BspANI GGCC 3 cut(s) 343, 1700, 1935
BspFNI CGCG 2 cut(s) 532, 1132
BspLI GGNNCC 3 cut(s) 122, 1804, 2145
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 1 cut(s) 1565
BspPI GGATC 1 cut(s) 1530
BspQI GCTCTTC 2 cut(s) 17, 397
BspT104I TTCGAA 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 1802
BspTI CTTAAG 2 cut(s) 1217, 2363
BsrDI GCAATG 2 cut(s) 248, 2313
BsrFI RCCGGY 2 cut(s) 123, 1773
BsrI ACTGG 8 cut(s) 505, 1113, 1128, 1214, 1701, 1891, 2073, 2640
BssAI RCCGGY 2 cut(s) 123, 1773
BssECI CCNNGG 4 cut(s) 560, 650, 1629, 1954
BssMI GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
BssNI GRCGYC 2 cut(s) 523, 2408
BssSI CACGAG 1 cut(s) 1839
BssT1I CCWWGG 2 cut(s) 560, 1954
Bst2BI CACGAG 1 cut(s) 1839
Bst2UI CCWGG 3 cut(s) 345, 798, 2405
Bst4CI ACNGT 7 cut(s) 434, 479, 690, 845, 1065, 1475, 2428
Bst6I CTCTTC 5 cut(s) 17, 397, 867, 1315, 1749
BstACI GRCGYC 2 cut(s) 523, 2408
BstAFI CTTAAG 2 cut(s) 1217, 2363
BstBAI YACGTR 1 cut(s) 713
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 6 cut(s) 660, 818, 1275, 1589, 1933, 2247
BstDSI CCRYGG 2 cut(s) 1629, 1954
BstENI CCTNNNNNAGG 2 cut(s) 1705, 2561
BstF5I GGATG 7 cut(s) 524, 608, 889, 1482, 1969, 2128, 2158
BstFNI CGCG 2 cut(s) 532, 1132
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 8 cut(s) 26, 203, 770, 775, 1525, 2140, 2550, 2592
BstMAI GTCTC 9 cut(s) 484, 575, 696, 860, 1294, 1312, 1849, 2330, 2460
BstMBI GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
BstMWI GCNNNNNNNGC 6 cut(s) 529, 1082, 1258, 1279, 1669, 2255
BstNI CCWGG 3 cut(s) 345, 798, 2405
BstNSI RCATGY 2 cut(s) 2512, 2536
BstSCI CCNGG 4 cut(s) 343, 650, 796, 2403
BstSFI CTRYAG 1 cut(s) 818
BstSLI GKGCMC 1 cut(s) 2108
BstUI CGCG 2 cut(s) 532, 1132
BstV1I GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
BstV2I GAAGAC 3 cut(s) 590, 1476, 1580
BstX2I RGATCY 4 cut(s) 23, 200, 772, 1522
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 4 cut(s) 23, 200, 772, 1522
BsuI GTATCC 1 cut(s) 2433
BsuRI GGCC 3 cut(s) 343, 1700, 1935
BtgI CCRYGG 2 cut(s) 1629, 1954
BtrI CACGTC 1 cut(s) 1651
BtsCI GGATG 7 cut(s) 524, 608, 889, 1482, 1969, 2128, 2158
BtsIMutI CAGTG 1 cut(s) 992
BtuMI TCGCGA 1 cut(s) 1132
BveI ACCTGC 1 cut(s) 1565
Cac8I GCNNGC 6 cut(s) 660, 818, 1275, 1589, 1933, 2247
CaiI CAGNNNCTG 1 cut(s) 2264
CfoI GCGC 1 cut(s) 372
Cfr10I RCCGGY 2 cut(s) 123, 1773
Cfr13I GGNCC 3 cut(s) 430, 2120, 2344
CseI GACGC 2 cut(s) 512, 2397
Csp6I GTAC 1 cut(s) 979
CspAI ACCGGT 1 cut(s) 1773
CviQI GTAC 1 cut(s) 979
DpnI GATC 8 cut(s) 25, 202, 769, 774, 1524, 2139, 2549, 2591
DpnII GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
DrdI GACNNNNNNGTC 1 cut(s) 1210
DriI GACNNNNNGTC 2 cut(s) 1198, 2635
DseDI GACNNNNNNGTC 1 cut(s) 1210
Eam1104I CTCTTC 5 cut(s) 17, 397, 867, 1315, 1749
Eam1105I GACNNNNNGTC 2 cut(s) 1198, 2635
EarI CTCTTC 5 cut(s) 17, 397, 867, 1315, 1749
Ecl136II GAGCTC 3 cut(s) 390, 1887, 2170
Eco130I CCWWGG 2 cut(s) 560, 1954
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 4 cut(s) 392, 568, 1889, 2172
Eco32I GATATC 1 cut(s) 78
Eco47I GGWCC 3 cut(s) 430, 2120, 2344
Eco53kI GAGCTC 3 cut(s) 390, 1887, 2170
Eco57I CTGAAG 2 cut(s) 891, 2375
Eco72I CACGTG 1 cut(s) 713
Eco88I CYCGRG 1 cut(s) 2433
EcoICRI GAGCTC 3 cut(s) 390, 1887, 2170
EcoNI CCTNNNNNAGG 2 cut(s) 1705, 2561
EcoO109I RGGNCCY 1 cut(s) 2344
EcoRII CCWGG 3 cut(s) 343, 796, 2403
EcoRV GATATC 1 cut(s) 78
EcoT14I CCWWGG 2 cut(s) 560, 1954
EcoT38I GRGCYC 4 cut(s) 392, 568, 1889, 2172
ErhI CCWWGG 2 cut(s) 560, 1954
FalI AAGNNNNNCTT 4 cut(s) 28, 31, 2238, 2270
FaqI GGGAC 1 cut(s) 2628
FbaI TGATCA 1 cut(s) 2137
FblI GTMKAC 2 cut(s) 1202, 2197
Fnu4HI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
FokI GGATG 7 cut(s) 531, 595, 896, 1469, 1976, 2115, 2165
FriOI GRGCYC 4 cut(s) 392, 568, 1889, 2172
Fsp4HI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
FspBI CTAG 7 cut(s) 36, 363, 405, 561, 1190, 1820, 1910
GlaI GCGC 1 cut(s) 371
GluI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
GsuI CTGGAG 4 cut(s) 819, 1145, 2130, 2387
HaeIII GGCC 3 cut(s) 343, 1700, 1935
HapII CCGG 4 cut(s) 107, 124, 652, 1774
HgaI GACGC 2 cut(s) 512, 2397
HhaI GCGC 1 cut(s) 372
Hin1I GRCGYC 2 cut(s) 523, 2408
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 4 cut(s) 1203, 1683, 2412, 2530
HindII GTYRAC 4 cut(s) 1203, 1683, 2412, 2530
HindIII AAGCTT 5 cut(s) 958, 1033, 1220, 1727, 2023
HpaI GTTAAC 1 cut(s) 2530
HpaII CCGG 4 cut(s) 107, 124, 652, 1774
HphI GGTGA 6 cut(s) 549, 736, 848, 1645, 2320, 2497
Hpy188III TCNNGA 9 cut(s) 278, 363, 1131, 1256, 1415, 1943, 2132, 2147, 2645
HpyCH4III ACNGT 7 cut(s) 434, 479, 690, 845, 1065, 1475, 2428
HpyCH4IV ACGT 3 cut(s) 696, 712, 1650
HpyF10VI GCNNNNNNNGC 6 cut(s) 529, 1082, 1258, 1279, 1669, 2255
HpySE526I ACGT 3 cut(s) 696, 712, 1650
Hsp92I GRCGYC 2 cut(s) 523, 2408
HspAI GCGC 1 cut(s) 370
Ksp22I TGATCA 1 cut(s) 2137
KspAI GTTAAC 1 cut(s) 2530
Kzo9I GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
LguI GCTCTTC 2 cut(s) 17, 397
LmnI GCTCC 7 cut(s) 120, 225, 387, 1243, 1669, 1892, 2149
Lsp1109I GCAGC 5 cut(s) 152, 1472, 2046, 2270, 2273
LweI GCATC 4 cut(s) 73, 1491, 2105, 2349
MaeI CTAG 7 cut(s) 36, 363, 405, 561, 1190, 1820, 1910
MaeII ACGT 3 cut(s) 696, 712, 1650
MaeIII GTNAC 6 cut(s) 434, 1212, 1381, 1651, 2396, 2428
MalI GATC 8 cut(s) 25, 202, 769, 774, 1524, 2139, 2549, 2591
MboI GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
MflI RGATCY 4 cut(s) 23, 200, 772, 1522
MhlI GDGCHC 5 cut(s) 392, 568, 1889, 2108, 2172
MlyI GAGTC 2 cut(s) 1136, 1851
MmeI TCCRAC 1 cut(s) 1695
MroXI GAANNNNTTC 3 cut(s) 11, 827, 960
MseI TTAA 8 cut(s) 1218, 1317, 1371, 1808, 1920, 2364, 2423, 2529
MslI CAYNNNNRTG 2 cut(s) 2101, 2133
MspA1I CMGCKG 1 cut(s) 2264
MspCI CTTAAG 2 cut(s) 1217, 2363
MspI CCGG 4 cut(s) 107, 124, 652, 1774
MspR9I CCNGG 4 cut(s) 345, 652, 798, 2405
Mva1269I GAATGC 2 cut(s) 372, 2116
MvaI CCWGG 3 cut(s) 345, 798, 2405
MvnI CGCG 2 cut(s) 532, 1132
MwoI GCNNNNNNNGC 6 cut(s) 529, 1082, 1258, 1279, 1669, 2255
NciI CCSGG 1 cut(s) 652
NcoI CCATGG 1 cut(s) 1954
NdeII GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
NlaIV GGNNCC 3 cut(s) 122, 1804, 2145
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 3 cut(s) 1212, 1651, 2396
NruI TCGCGA 1 cut(s) 1132
NspI RCATGY 2 cut(s) 2512, 2536
NspV TTCGAA 1 cut(s) 185
PceI AGGCCT 1 cut(s) 343
PciI ACATGT 2 cut(s) 2508, 2532
PciSI GCTCTTC 2 cut(s) 17, 397
PctI GAATGC 2 cut(s) 372, 2116
PdmI GAANNNNTTC 3 cut(s) 11, 827, 960
PfeI GAWTC 8 cut(s) 377, 644, 997, 1411, 1718, 1739, 1858, 1982
PflFI GACNNNGTC 1 cut(s) 2047
PflMI CCANNNNNTGG 2 cut(s) 1630, 1960
PfoI TCCNGGA 1 cut(s) 796
PinAI ACCGGT 1 cut(s) 1773
PkrI GCNGC 5 cut(s) 142, 1462, 2061, 2260, 2263
PleI GAGTC 2 cut(s) 1135, 1850
PmaCI CACGTG 1 cut(s) 713
PmlI CACGTG 1 cut(s) 713
PpsI GAGTC 2 cut(s) 1135, 1850
Ppu21I YACGTR 1 cut(s) 713
PpuMI RGGWCCY 1 cut(s) 2344
PscI ACATGT 2 cut(s) 2508, 2532
Psp124BI GAGCTC 3 cut(s) 392, 1889, 2172
Psp5II RGGWCCY 1 cut(s) 2344
Psp6I CCWGG 3 cut(s) 343, 796, 2403
PspCI CACGTG 1 cut(s) 713
PspGI CCWGG 3 cut(s) 343, 796, 2403
PspN4I GGNNCC 3 cut(s) 122, 1804, 2145
PspPI GGNCC 3 cut(s) 430, 2120, 2344
PspPPI RGGWCCY 1 cut(s) 2344
PsrI GAACNNNNNNTAC 2 cut(s) 526, 558
PstI CTGCAG 1 cut(s) 822
PstNI CAGNNNCTG 1 cut(s) 2264
PsuI RGATCY 4 cut(s) 23, 200, 772, 1522
PsyI GACNNNGTC 1 cut(s) 2047
PvuII CAGCTG 1 cut(s) 2264
RruI TCGCGA 1 cut(s) 1132
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
RseI CAYNNNNRTG 2 cut(s) 2101, 2133
SacI GAGCTC 3 cut(s) 392, 1889, 2172
SalI GTCGAC 1 cut(s) 1201
SapI GCTCTTC 2 cut(s) 17, 397
SaqAI TTAA 8 cut(s) 1218, 1317, 1371, 1808, 1920, 2364, 2423, 2529
SatI GCNGC 5 cut(s) 141, 1461, 2060, 2259, 2262
Sau3AI GATC 8 cut(s) 23, 200, 767, 772, 1522, 2137, 2547, 2589
Sau96I GGNCC 3 cut(s) 430, 2120, 2344
SchI GAGTC 2 cut(s) 1136, 1851
ScrFI CCNGG 4 cut(s) 345, 652, 798, 2405
SduI GDGCHC 5 cut(s) 392, 568, 1889, 2108, 2172
SfaNI GCATC 4 cut(s) 73, 1491, 2105, 2349
SfcI CTRYAG 1 cut(s) 818
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 3 cut(s) 430, 2120, 2344
SmiMI CAYNNNNRTG 2 cut(s) 2101, 2133
SmlI CTYRAG 6 cut(s) 811, 1043, 1217, 1223, 1702, 2363
SmoI CTYRAG 6 cut(s) 811, 1043, 1217, 1223, 1702, 2363
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 1 cut(s) 530
SspMI CTAG 7 cut(s) 36, 363, 405, 561, 1190, 1820, 1910
SstI GAGCTC 3 cut(s) 392, 1889, 2172
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 4 cut(s) 343, 650, 796, 2403
StyI CCWWGG 2 cut(s) 560, 1954
TaaI ACNGT 7 cut(s) 434, 479, 690, 845, 1065, 1475, 2428
TaiI ACGT 3 cut(s) 699, 715, 1653
TaqI TCGA 5 cut(s) 185, 265, 1202, 2550, 2628
TfiI GAWTC 8 cut(s) 377, 644, 997, 1411, 1718, 1739, 1858, 1982
Tru1I TTAA 8 cut(s) 1218, 1317, 1371, 1808, 1920, 2364, 2423, 2529
Tru9I TTAA 8 cut(s) 1218, 1317, 1371, 1808, 1920, 2364, 2423, 2529
TscAI CASTG 1 cut(s) 999
TseFI GTSAC 3 cut(s) 1212, 1651, 2396
TseI GCWGC 5 cut(s) 140, 1460, 2059, 2258, 2261
Tsp45I GTSAC 3 cut(s) 1212, 1651, 2396
TspDTI ATGAA 9 cut(s) 17, 561, 594, 657, 1868, 2006, 2079, 2310, 2657
TspGWI ACGGA 3 cut(s) 459, 1131, 1618
TspRI CASTG 1 cut(s) 999
Tth111I GACNNNGTC 1 cut(s) 2047
Van91I CCANNNNNTGG 2 cut(s) 1630, 1960
Vha464I CTTAAG 2 cut(s) 1217, 2363
VneI GTGCAC 1 cut(s) 2104
VpaK11BI GGWCC 3 cut(s) 430, 2120, 2344
XagI CCTNNNNNAGG 2 cut(s) 1705, 2561
XapI RAATTY 6 cut(s) 1492, 1610, 1903, 1913, 2451, 2657
XbaI TCTAGA 1 cut(s) 362
XceI RCATGY 2 cut(s) 2512, 2536
XcmI CCANNNNNNNNNTGG 3 cut(s) 326, 1663, 1961
XmaJI CCTAGG 1 cut(s) 560
XmiI GTMKAC 2 cut(s) 1202, 2197
XmnI GAANNNNTTC 3 cut(s) 11, 827, 960
XspI CTAG 7 cut(s) 36, 363, 405, 561, 1190, 1820, 1910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.