Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G55630 AT3G55630 AT3G55630 AT3G55630 AT3G55630 AT3G55630 AT3G55630 AT5G05980 AT5G05980 |
| fragaria_vesca | FvH4_7g23490 FvH4_7g23490 FvH4_7g23490 FvH4_7g23490 FvH4_7g23490 FvH4_7g23490 FvH4_7g23490 FvH4_7g23490 |
| malus_domestica | MD01G1140600.v1.1 MD07G1204900.v1.1 |
| prunus_persica | Prupe.2G241100_v2.0.a1 Prupe.2G241100_v2.0.a1 |
| pyrus_communis | pycom01g16120 pycom07g19140 |
| rosa_chinensis | RchiOBHm_Chr1g0368621 |
| rosa_laevigata | RLG00000027200 |
| rosa_multiflora | Rmu_sc0011394.1_g000002 |
| rosa_roxburghii | Rroxscaffold_4G00288400 |
| rosa_rugosa | Rorug01G0343200 |
| rosa_samantha | Rh1AG350500 Rh1BG313800 Rh1CG328400 Rh1DG345200 |
| rosa_wichuraiana | Rw0G021640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 239 |
| Acc16I | TGCGCA | 1 cut(s) | 1039 |
| Acc36I | ACCTGC | 1 cut(s) | 239 |
| AccB1I | GGYRCC | 3 cut(s) | 209, 541, 1107 |
| AccI | GTMKAC | 1 cut(s) | 1227 |
| AciI | CCGC | 7 cut(s) | 5, 35, 127, 264, 510, 743, 915 |
| AclWI | GGATC | 9 cut(s) | 219, 232, 743, 756, 1088, 1090, 1169, 1182, 1436 |
| AcsI | RAATTY | 2 cut(s) | 353, 1132 |
| AcuI | CTGAAG | 1 cut(s) | 1002 |
| AcyI | GRCGYC | 1 cut(s) | 542 |
| AfaI | GTAC | 7 cut(s) | 53, 666, 693, 738, 1075, 1101, 1251 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 166, 733, 837 |
| AflII | CTTAAG | 1 cut(s) | 389 |
| AflIII | ACRYGT | 2 cut(s) | 1208, 1569 |
| AgsI | TTSAA | 7 cut(s) | 182, 640, 731, 863, 1118, 1234, 1340 |
| AjiI | CACGTC | 2 cut(s) | 288, 1570 |
| AjnI | CCWGG | 1 cut(s) | 159 |
| AleI | CACNNNNGTG | 1 cut(s) | 1262 |
| AluBI | AGCT | 1 cut(s) | 1481 |
| AluI | AGCT | 1 cut(s) | 1481 |
| Alw21I | GWGCWC | 2 cut(s) | 795, 1012 |
| Alw26I | GTCTC | 2 cut(s) | 294, 379 |
| Alw44I | GTGCAC | 1 cut(s) | 1008 |
| AlwI | GGATC | 9 cut(s) | 219, 232, 743, 756, 1088, 1090, 1169, 1182, 1436 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1147 |
| ApaLI | GTGCAC | 1 cut(s) | 1008 |
| ApeKI | GCWGC | 2 cut(s) | 467, 1391 |
| ApoI | RAATTY | 2 cut(s) | 353, 1132 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 125, 157 |
| AseI | ATTAAT | 1 cut(s) | 1580 |
| AspLEI | GCGC | 3 cut(s) | 544, 1040, 1379 |
| AspS9I | GGNCC | 1 cut(s) | 1534 |
| AsuHPI | GGTGA | 2 cut(s) | 24, 848 |
| AvaII | GGWCC | 1 cut(s) | 1534 |
| BaeGI | GKGCMC | 1 cut(s) | 1012 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1136, 1169 |
| BamHI | GGATCC | 3 cut(s) | 224, 748, 1174 |
| BanI | GGYRCC | 3 cut(s) | 209, 541, 1107 |
| BanII | GRGCYC | 1 cut(s) | 943 |
| Bbv12I | GWGCWC | 2 cut(s) | 795, 1012 |
| BbvI | GCAGC | 2 cut(s) | 479, 1403 |
| BccI | CCATC | 4 cut(s) | 14, 323, 1038, 1483 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1436, 1470 |
| BciT130I | CCWGG | 1 cut(s) | 161 |
| BclI | TGATCA | 1 cut(s) | 84 |
| BcoDI | GTCTC | 2 cut(s) | 294, 379 |
| BfaI | CTAG | 2 cut(s) | 645, 1215 |
| BfmI | CTRYAG | 1 cut(s) | 918 |
| BfoI | RGCGCY | 2 cut(s) | 545, 1380 |
| BfrI | CTTAAG | 1 cut(s) | 389 |
| BfuAI | ACCTGC | 1 cut(s) | 239 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 264 |
| BglII | AGATCT | 1 cut(s) | 985 |
| BisI | GCNGC | 3 cut(s) | 265, 468, 1392 |
| BlsI | GCNGC | 3 cut(s) | 266, 469, 1393 |
| BmcAI | AGTACT | 1 cut(s) | 1075 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| Bme18I | GGWCC | 1 cut(s) | 1534 |
| BmgBI | CACGTC | 2 cut(s) | 288, 1570 |
| BmgT120I | GGNCC | 1 cut(s) | 1534 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BmrI | ACTGGG | 1 cut(s) | 1065 |
| BmsI | GCATC | 1 cut(s) | 511 |
| BmuI | ACTGGG | 1 cut(s) | 1065 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1162, 1194 |
| BpmI | CTGGAG | 2 cut(s) | 182, 827 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1493 |
| BpuEI | CTTGAG | 1 cut(s) | 1293 |
| BsaHI | GRCGYC | 1 cut(s) | 542 |
| BsaJI | CCNNGG | 2 cut(s) | 568, 1395 |
| BsaWI | WCCGGW | 2 cut(s) | 321, 1096 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 488, 518 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 166, 733, 837 |
| Bse118I | RCCGGY | 1 cut(s) | 206 |
| Bse1I | ACTGG | 7 cut(s) | 69, 280, 699, 738, 856, 1071, 1549 |
| Bse3DI | GCAATG | 2 cut(s) | 259, 690 |
| BseBI | CCWGG | 1 cut(s) | 161 |
| BseDI | CCNNGG | 2 cut(s) | 568, 1395 |
| BseGI | GGATG | 3 cut(s) | 192, 334, 724 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 166, 733, 837 |
| BseMI | GCAATG | 2 cut(s) | 259, 690 |
| BseMII | CTCAG | 3 cut(s) | 725, 1193, 1507 |
| BseNI | ACTGG | 7 cut(s) | 69, 280, 699, 738, 856, 1071, 1549 |
| BseRI | GAGGAG | 4 cut(s) | 16, 128, 185, 282 |
| BseSI | GKGCMC | 1 cut(s) | 1012 |
| BseXI | GCAGC | 2 cut(s) | 479, 1403 |
| BseYI | CCCAGC | 1 cut(s) | 617 |
| BsgI | GTGCAG | 3 cut(s) | 217, 1410, 1476 |
| Bsh1285I | CGRYCG | 1 cut(s) | 127 |
| BshNI | GGYRCC | 3 cut(s) | 209, 541, 1107 |
| BsiEI | CGRYCG | 1 cut(s) | 127 |
| BsiHKAI | GWGCWC | 2 cut(s) | 795, 1012 |
| BsiSI | CCGG | 5 cut(s) | 41, 207, 322, 890, 1097 |
| BsiWI | CGTACG | 1 cut(s) | 51 |
| BslFI | GGGAC | 2 cut(s) | 935, 1511 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 166, 733, 837 |
| BsmAI | GTCTC | 2 cut(s) | 294, 379 |
| BsmBI | CGTCTC | 1 cut(s) | 294 |
| BsmFI | GGGAC | 2 cut(s) | 935, 1511 |
| Bsp1286I | GDGCHC | 3 cut(s) | 795, 943, 1012 |
| Bsp143I | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| BspACI | CCGC | 7 cut(s) | 5, 35, 127, 264, 510, 743, 915 |
| BspCNI | CTCAG | 3 cut(s) | 724, 1192, 1506 |
| BspMI | ACCTGC | 1 cut(s) | 239 |
| BspPI | GGATC | 9 cut(s) | 219, 232, 743, 756, 1088, 1090, 1169, 1182, 1436 |
| BspQI | GCTCTTC | 1 cut(s) | 1341 |
| BspT107I | GGYRCC | 3 cut(s) | 209, 541, 1107 |
| BspTI | CTTAAG | 1 cut(s) | 389 |
| BsrDI | GCAATG | 2 cut(s) | 259, 690 |
| BsrFI | RCCGGY | 1 cut(s) | 206 |
| BsrI | ACTGG | 7 cut(s) | 69, 280, 699, 738, 856, 1071, 1549 |
| BssAI | RCCGGY | 1 cut(s) | 206 |
| BssECI | CCNNGG | 2 cut(s) | 568, 1395 |
| BssMI | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| BssNI | GRCGYC | 1 cut(s) | 542 |
| BssT1I | CCWWGG | 1 cut(s) | 568 |
| Bst2UI | CCWGG | 1 cut(s) | 161 |
| Bst4CI | ACNGT | 7 cut(s) | 664, 828, 922, 971, 1007, 1399, 1504 |
| Bst6I | CTCTTC | 4 cut(s) | 146, 342, 693, 1341 |
| BstACI | GRCGYC | 1 cut(s) | 542 |
| BstAFI | CTTAAG | 1 cut(s) | 389 |
| BstC8I | GCNNGC | 5 cut(s) | 208, 450, 783, 787, 791 |
| BstDEI | CTNAG | 3 cut(s) | 711, 1179, 1493 |
| BstDSI | CCRYGG | 1 cut(s) | 1395 |
| BstEII | GGTNACC | 1 cut(s) | 854 |
| BstF5I | GGATG | 3 cut(s) | 192, 334, 724 |
| BstH2I | RGCGCY | 2 cut(s) | 545, 1380 |
| BstHHI | GCGC | 3 cut(s) | 544, 1040, 1379 |
| BstKTI | GATC | 9 cut(s) | 87, 227, 751, 958, 988, 1085, 1096, 1177, 1431 |
| BstMAI | GTCTC | 2 cut(s) | 294, 379 |
| BstMBI | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| BstMCI | CGRYCG | 1 cut(s) | 127 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 68, 120, 216, 264, 270, 449, 815, 1023 |
| BstNI | CCWGG | 1 cut(s) | 161 |
| BstNSI | RCATGY | 1 cut(s) | 1212 |
| BstPI | GGTNACC | 1 cut(s) | 854 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstSFI | CTRYAG | 1 cut(s) | 918 |
| BstSLI | GKGCMC | 1 cut(s) | 1012 |
| BstV1I | GCAGC | 2 cut(s) | 479, 1403 |
| BstX2I | RGATCY | 4 cut(s) | 224, 748, 985, 1174 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 851 |
| BstYI | RGATCY | 4 cut(s) | 224, 748, 985, 1174 |
| BtgI | CCRYGG | 1 cut(s) | 1395 |
| BtgZI | GCGATG | 1 cut(s) | 33 |
| BtrI | CACGTC | 2 cut(s) | 288, 1570 |
| BtsCI | GGATG | 3 cut(s) | 192, 334, 724 |
| BtsIMutI | CAGTG | 8 cut(s) | 62, 273, 349, 824, 976, 1173, 1269, 1500 |
| BveI | ACCTGC | 1 cut(s) | 239 |
| Cac8I | GCNNGC | 5 cut(s) | 208, 450, 783, 787, 791 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1147 |
| CfoI | GCGC | 3 cut(s) | 544, 1040, 1379 |
| Cfr10I | RCCGGY | 1 cut(s) | 206 |
| Cfr13I | GGNCC | 1 cut(s) | 1534 |
| CseI | GACGC | 1 cut(s) | 77 |
| Csp6I | GTAC | 7 cut(s) | 52, 665, 692, 737, 1074, 1100, 1250 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 513, 548 |
| CviAII | CATG | 6 cut(s) | 583, 837, 1027, 1091, 1209, 1220 |
| CviQI | GTAC | 7 cut(s) | 52, 665, 692, 737, 1074, 1100, 1250 |
| DdeI | CTNAG | 3 cut(s) | 711, 1179, 1493 |
| DinI | GGCGCC | 1 cut(s) | 543 |
| DpnI | GATC | 9 cut(s) | 86, 226, 750, 957, 987, 1084, 1095, 1176, 1430 |
| DpnII | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| Eam1104I | CTCTTC | 4 cut(s) | 146, 342, 693, 1341 |
| EarI | CTCTTC | 4 cut(s) | 146, 342, 693, 1341 |
| EciI | GGCGGA | 2 cut(s) | 20, 525 |
| Eco130I | CCWWGG | 1 cut(s) | 568 |
| Eco24I | GRGCYC | 1 cut(s) | 943 |
| Eco47I | GGWCC | 1 cut(s) | 1534 |
| Eco57I | CTGAAG | 1 cut(s) | 1002 |
| Eco91I | GGTNACC | 1 cut(s) | 854 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1534 |
| EcoO65I | GGTNACC | 1 cut(s) | 854 |
| EcoRI | GAATTC | 1 cut(s) | 1132 |
| EcoRII | CCWGG | 1 cut(s) | 159 |
| EcoT14I | CCWWGG | 1 cut(s) | 568 |
| EcoT38I | GRGCYC | 1 cut(s) | 943 |
| EgeI | GGCGCC | 1 cut(s) | 543 |
| EheI | GGCGCC | 1 cut(s) | 543 |
| ErhI | CCWWGG | 1 cut(s) | 568 |
| Esp3I | CGTCTC | 1 cut(s) | 294 |
| FaeI | CATG | 6 cut(s) | 586, 840, 1030, 1094, 1212, 1223 |
| FaqI | GGGAC | 2 cut(s) | 935, 1511 |
| FatI | CATG | 6 cut(s) | 582, 836, 1026, 1090, 1208, 1219 |
| FbaI | TGATCA | 1 cut(s) | 84 |
| FblI | GTMKAC | 1 cut(s) | 1227 |
| Fnu4HI | GCNGC | 3 cut(s) | 265, 468, 1392 |
| FokI | GGATG | 3 cut(s) | 199, 341, 731 |
| FriOI | GRGCYC | 1 cut(s) | 943 |
| Fsp4HI | GCNGC | 3 cut(s) | 265, 468, 1392 |
| FspBI | CTAG | 2 cut(s) | 645, 1215 |
| FspI | TGCGCA | 1 cut(s) | 1039 |
| GlaI | GCGC | 3 cut(s) | 543, 1039, 1378 |
| GluI | GCNGC | 3 cut(s) | 265, 468, 1392 |
| GsaI | CCCAGC | 1 cut(s) | 621 |
| GsuI | CTGGAG | 2 cut(s) | 182, 827 |
| HaeII | RGCGCY | 2 cut(s) | 545, 1380 |
| HapII | CCGG | 5 cut(s) | 41, 207, 322, 890, 1097 |
| HgaI | GACGC | 1 cut(s) | 77 |
| HhaI | GCGC | 3 cut(s) | 544, 1040, 1379 |
| Hin1I | GRCGYC | 1 cut(s) | 542 |
| Hin1II | CATG | 6 cut(s) | 586, 840, 1030, 1094, 1212, 1223 |
| Hin6I | GCGC | 3 cut(s) | 542, 1038, 1377 |
| HinP1I | GCGC | 3 cut(s) | 542, 1038, 1377 |
| HinfI | GANTC | 8 cut(s) | 8, 46, 245, 318, 589, 633, 1195, 1304 |
| HpaII | CCGG | 5 cut(s) | 41, 207, 322, 890, 1097 |
| HphI | GGTGA | 2 cut(s) | 24, 848 |
| Hpy166II | GTNNAC | 5 cut(s) | 692, 929, 1010, 1228, 1283 |
| Hpy188I | TCNGA | 4 cut(s) | 1138, 1194, 1200, 1508 |
| Hpy188III | TCNNGA | 6 cut(s) | 311, 752, 844, 1080, 1234, 1585 |
| Hpy8I | GTNNAC | 5 cut(s) | 692, 929, 1010, 1228, 1283 |
| Hpy99I | CGWCG | 1 cut(s) | 1571 |
| HpyAV | CCTTC | 7 cut(s) | 52, 247, 507, 619, 697, 737, 1231 |
| HpyCH4III | ACNGT | 7 cut(s) | 664, 828, 922, 971, 1007, 1399, 1504 |
| HpyCH4IV | ACGT | 3 cut(s) | 201, 287, 1569 |
| HpyCH4V | TGCA | 8 cut(s) | 234, 524, 785, 824, 1010, 1363, 1391, 1457 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 68, 120, 216, 264, 270, 449, 815, 1023 |
| HpyF3I | CTNAG | 3 cut(s) | 711, 1179, 1493 |
| HpySE526I | ACGT | 3 cut(s) | 201, 287, 1569 |
| Hsp92I | GRCGYC | 1 cut(s) | 542 |
| Hsp92II | CATG | 6 cut(s) | 586, 840, 1030, 1094, 1212, 1223 |
| HspAI | GCGC | 3 cut(s) | 542, 1038, 1377 |
| KasI | GGCGCC | 1 cut(s) | 541 |
| KroI | GCCGGC | 1 cut(s) | 206 |
| KroNI | GCCGGC | 1 cut(s) | 208 |
| Ksp22I | TGATCA | 1 cut(s) | 84 |
| Kzo9I | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| LguI | GCTCTTC | 1 cut(s) | 1341 |
| LmnI | GCTCC | 5 cut(s) | 29, 172, 608, 846, 892 |
| Lsp1109I | GCAGC | 2 cut(s) | 479, 1403 |
| LweI | GCATC | 1 cut(s) | 511 |
| MaeI | CTAG | 2 cut(s) | 645, 1215 |
| MaeII | ACGT | 3 cut(s) | 201, 287, 1569 |
| MaeIII | GTNAC | 5 cut(s) | 30, 854, 1367, 1540, 1551 |
| MalI | GATC | 9 cut(s) | 86, 226, 750, 957, 987, 1084, 1095, 1176, 1430 |
| MboI | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| MfeI | CAATTG | 2 cut(s) | 177, 612 |
| MflI | RGATCY | 4 cut(s) | 224, 748, 985, 1174 |
| MhlI | GDGCHC | 3 cut(s) | 795, 943, 1012 |
| MluCI | AATT | 5 cut(s) | 177, 353, 612, 1132, 1156 |
| Mly113I | GGCGCC | 1 cut(s) | 542 |
| MlyI | GAGTC | 2 cut(s) | 40, 254 |
| MmeI | TCCRAC | 5 cut(s) | 147, 306, 696, 1322, 1531 |
| MroNI | GCCGGC | 1 cut(s) | 206 |
| MseI | TTAA | 6 cut(s) | 390, 456, 804, 966, 1155, 1580 |
| MslI | CAYNNNNRTG | 2 cut(s) | 798, 1262 |
| MspA1I | CMGCKG | 1 cut(s) | 745 |
| MspCI | CTTAAG | 1 cut(s) | 389 |
| MspI | CCGG | 5 cut(s) | 41, 207, 322, 890, 1097 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| MunI | CAATTG | 2 cut(s) | 177, 612 |
| MvaI | CCWGG | 1 cut(s) | 161 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 68, 120, 216, 264, 270, 449, 815, 1023 |
| NaeI | GCCGGC | 1 cut(s) | 208 |
| NarI | GGCGCC | 1 cut(s) | 542 |
| NdeII | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| NgoMIV | GCCGGC | 1 cut(s) | 206 |
| NlaIII | CATG | 6 cut(s) | 586, 840, 1030, 1094, 1212, 1223 |
| NmuCI | GTSAC | 2 cut(s) | 30, 854 |
| NsbI | TGCGCA | 1 cut(s) | 1039 |
| NspI | RCATGY | 1 cut(s) | 1212 |
| OliI | CACNNNNGTG | 1 cut(s) | 1262 |
| PaqCI | CACCTGC | 1 cut(s) | 239 |
| PciI | ACATGT | 1 cut(s) | 1208 |
| PciSI | GCTCTTC | 1 cut(s) | 1341 |
| PdiI | GCCGGC | 1 cut(s) | 208 |
| PfeI | GAWTC | 6 cut(s) | 8, 318, 589, 633, 1195, 1304 |
| Pfl23II | CGTACG | 1 cut(s) | 51 |
| PflFI | GACNNNGTC | 1 cut(s) | 1502 |
| PfoI | TCCNGGA | 1 cut(s) | 159 |
| PkrI | GCNGC | 3 cut(s) | 266, 469, 1393 |
| PleI | GAGTC | 2 cut(s) | 40, 253 |
| PluTI | GGCGCC | 1 cut(s) | 545 |
| PpsI | GAGTC | 2 cut(s) | 40, 253 |
| PpuMI | RGGWCCY | 1 cut(s) | 1534 |
| PscI | ACATGT | 1 cut(s) | 1208 |
| PshBI | ATTAAT | 1 cut(s) | 1580 |
| Psp5II | RGGWCCY | 1 cut(s) | 1534 |
| Psp6I | CCWGG | 1 cut(s) | 159 |
| PspEI | GGTNACC | 1 cut(s) | 854 |
| PspFI | CCCAGC | 1 cut(s) | 617 |
| PspGI | CCWGG | 1 cut(s) | 159 |
| PspLI | CGTACG | 1 cut(s) | 51 |
| PspPI | GGNCC | 1 cut(s) | 1534 |
| PspPPI | RGGWCCY | 1 cut(s) | 1534 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1147 |
| PsuI | RGATCY | 4 cut(s) | 224, 748, 985, 1174 |
| PsyI | GACNNNGTC | 1 cut(s) | 1502 |
| RsaI | GTAC | 7 cut(s) | 53, 666, 693, 738, 1075, 1101, 1251 |
| RsaNI | GTAC | 7 cut(s) | 52, 665, 692, 737, 1074, 1100, 1250 |
| RseI | CAYNNNNRTG | 2 cut(s) | 798, 1262 |
| SapI | GCTCTTC | 1 cut(s) | 1341 |
| SaqAI | TTAA | 6 cut(s) | 390, 456, 804, 966, 1155, 1580 |
| SatI | GCNGC | 3 cut(s) | 265, 468, 1392 |
| Sau3AI | GATC | 9 cut(s) | 84, 224, 748, 955, 985, 1082, 1093, 1174, 1428 |
| Sau96I | GGNCC | 1 cut(s) | 1534 |
| ScaI | AGTACT | 1 cut(s) | 1075 |
| SchI | GAGTC | 2 cut(s) | 40, 254 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SduI | GDGCHC | 3 cut(s) | 795, 943, 1012 |
| SfaNI | GCATC | 1 cut(s) | 511 |
| SfcI | CTRYAG | 1 cut(s) | 918 |
| SfoI | GGCGCC | 1 cut(s) | 543 |
| SinI | GGWCC | 1 cut(s) | 1534 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 798, 1262 |
| SmlI | CTYRAG | 2 cut(s) | 389, 1308 |
| SmoI | CTYRAG | 2 cut(s) | 389, 1308 |
| Sse9I | AATT | 5 cut(s) | 177, 353, 612, 1132, 1156 |
| SsiI | CCGC | 7 cut(s) | 5, 35, 127, 264, 510, 743, 915 |
| SspDI | GGCGCC | 1 cut(s) | 541 |
| SspMI | CTAG | 2 cut(s) | 645, 1215 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| StyI | CCWWGG | 1 cut(s) | 568 |
| TaaI | ACNGT | 7 cut(s) | 664, 828, 922, 971, 1007, 1399, 1504 |
| TaiI | ACGT | 3 cut(s) | 204, 290, 1572 |
| TaqI | TCGA | 4 cut(s) | 132, 144, 248, 310 |
| TasI | AATT | 5 cut(s) | 177, 353, 612, 1132, 1156 |
| TatI | WGTACW | 2 cut(s) | 691, 1073 |
| TauI | GCSGC | 1 cut(s) | 267 |
| TfiI | GAWTC | 6 cut(s) | 8, 318, 589, 633, 1195, 1304 |
| Tru1I | TTAA | 6 cut(s) | 390, 456, 804, 966, 1155, 1580 |
| Tru9I | TTAA | 6 cut(s) | 390, 456, 804, 966, 1155, 1580 |
| TscAI | CASTG | 8 cut(s) | 69, 280, 349, 831, 976, 1173, 1269, 1507 |
| TseFI | GTSAC | 2 cut(s) | 30, 854 |
| TseI | GCWGC | 2 cut(s) | 467, 1391 |
| Tsp45I | GTSAC | 2 cut(s) | 30, 854 |
| TspDTI | ATGAA | 4 cut(s) | 203, 693, 889, 1217 |
| TspGWI | ACGGA | 3 cut(s) | 39, 199, 1510 |
| TspRI | CASTG | 8 cut(s) | 69, 280, 349, 831, 976, 1173, 1269, 1507 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1502 |
| Vha464I | CTTAAG | 1 cut(s) | 389 |
| VneI | GTGCAC | 1 cut(s) | 1008 |
| VpaK11BI | GGWCC | 1 cut(s) | 1534 |
| VspI | ATTAAT | 1 cut(s) | 1580 |
| XapI | RAATTY | 2 cut(s) | 353, 1132 |
| XceI | RCATGY | 1 cut(s) | 1212 |
| XmiI | GTMKAC | 1 cut(s) | 1227 |
| XspI | CTAG | 2 cut(s) | 645, 1215 |
| ZrmI | AGTACT | 1 cut(s) | 1075 |