Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014283

Physical Location & Seq

Genomic Coordinates Forward (+)

30245362 .. 30250861

Gene Structure 5500 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1227000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1356 bp

ATGTGCTCCGTGTCTCTTCAGCCTCAGATCACACCTTCAAATACAATTATACGATTTCACATGAGACTAATGAAGCCACCGCCGCTCTCCTCCTCCTCCTCTGCCGCCGCCGTCTTCCGCAAGGCTCGACTCTCTCCCTACCTCTTCACTCTCCTCGTCTTCATCCTCTTCGTCTGCGTCCTCTACGGCGAGGATCTCACCTGCATTTTCGGCCAGCAGCTCCAACTCACCTCCAGCTCAGACCCAATCGTCATCAGAACAGGGAAGAAGAGAGAGAAGCTGCCGTTCGCCGTCGGCAAGACCGACGAAGGGTGTGACTTGTTCAGCGGGAGGTGGGTCTTCGACGAGTCAACTCGGCCGCTTTACGAGGAATCGGAGTGCCCGTACATCCAGCCACAGTTGACCTGCCAAGAACACGGGCGGCCCGACAAGGACTACCAGAAATGGCGGTGGCAGCCCCACGGCTGCGATCTTCCCAGCTTCAATGCCACTTTGATGCTCGAGACGCTACGAGGAAAGCGGATGATGTTCGTTGGAGACTCTCTGAACCGGGGTCAGTATGTTTCCATGATTTGTCTTCTCCACAAACTCATCCCGGATGATGCCAAATCCATGGAGAGCTTTGACTCAGACTCGCGAACTGTTTTCACAGCCAAGACATACAATGCCACAATCGAGTTCTATTGGGCGCCGTTTCTTCTTGAATCAAACGCGGATAATGCTGTCGTCCATAGGATATCTGACAGGCTTGTAAGGAAAGGGTCCGTCAATAAGCATGGCAAACATTGGAAGGGCGTTGATGTATTGGTGTTCAATACTTATCTGTGGTGGATGACCGGCTTGGAATTTAAAATATTACTAGGTTCCTTCAATGATGAGGTGAAAGATATTGTGAATGTGCCAACGACAGAGGCTTATCGTATGGCGATGAAAACTATGTTAAGATGGGTGAGGAGGAACATGGACCCCAAGAAGACGAGGGTCTTTTTCACTAGCATGTCTCCTTCTCATGGAAAGAGTATAGAATGGGGAGGTGAACCAGGAGGCAACTGTTACAACCAGACTACTTTGATTGAAGATCCAAACTACTGGGGTTCAGATTCCCGGAAAAACATAATGGAGGTAATTGGAGAAGTATTCGGTAAAACGACGGTGCCCATTTCTTTTCTCAACATCACGCAACTTTCAAACTATCGCAAGGATGCACACACATCAATCTACAAGAAGCAGTGGAGCCCTCTGACTCCCGAGCAATTAGCCAACCCTGTTAGCTATGCTGATTGCGTACATTGGTGTTTGCCTGGCCTTCAAGATACCTGGAATGAGCTTCTGTTCGCCAAACTTTTCTATCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044036 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1990538 GO:1990937

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

452

Amino Acids

51.81

Weight (kDa)

8.62

Isoelectric Point (pI)

46.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	104 - 157	9.3e-25	PMR5 N terminal Domain
PC-Esterase	PF13839	158 - 446	2.8e-88	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40320
fragaria_vesca	FvH4_7g25160 FvH4_7g25160
malus_domestica	MD01G1158300.v1.1 MD07G1227000.v1.1
prunus_persica	Prupe.2G256600_v2.0.a1
pyrus_communis	pycom01g17500 pycom07g20560
rosa_chinensis	RchiOBHm_Chr1g0371061
rosa_laevigata	RLG00000026989
rosa_multiflora	Rmu_sc0001018.1_g000029
rosa_roxburghii	Rroxscaffold_4G00286010 Rroxscaffold_4G00286020
rosa_rugosa	Rorug01G0361200
rosa_samantha	Rh1CG348200 Rh1DG366100
rosa_wichuraiana	Rw1G032670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	209
AasI	GACNNNNNNGTC	1 cut(s)	248
Acc36I	ACCTGC	2 cut(s)	209, 413
AccB1I	GGYRCC	2 cut(s)	688, 1153
AccBSI	CCGCTC	1 cut(s)	85
AccII	CGCG	2 cut(s)	637, 713
AclWI	GGATC	2 cut(s)	201, 1073
AcoI	YGGCCR	2 cut(s)	211, 356
AcsI	RAATTY	1 cut(s)	845
AcyI	GRCGYC	1 cut(s)	689
AfaI	GTAC	2 cut(s)	386, 1287
AfiI	CCNNNNNNNGG	2 cut(s)	309, 1010
AgsI	TTSAA	8 cut(s)	39, 484, 704, 814, 871, 1076, 1188, 1310
AjnI	CCWGG	3 cut(s)	1039, 1300, 1316
AluBI	AGCT	7 cut(s)	220, 237, 280, 480, 621, 1272, 1327
AluI	AGCT	7 cut(s)	220, 237, 280, 480, 621, 1272, 1327
Alw21I	GWGCWC	1 cut(s)	8
Alw26I	GTCTC	5 cut(s)	18, 58, 497, 531, 1005
AlwI	GGATC	2 cut(s)	201, 1073
Ama87I	CYCGRG	2 cut(s)	500, 1247
AoxI	GGCC	4 cut(s)	211, 356, 422, 1303
ApeKI	GCWGC	4 cut(s)	217, 280, 454, 465
ApoI	RAATTY	1 cut(s)	845
AspLEI	GCGC	1 cut(s)	691
AspS9I	GGNCC	3 cut(s)	423, 762, 964
AsuC2I	CCSGG	3 cut(s)	551, 596, 1105
AsuHPI	GGTGA	5 cut(s)	190, 220, 892, 961, 1046
AvaI	CYCGRG	2 cut(s)	500, 1247
AvaII	GGWCC	2 cut(s)	762, 964
BaeGI	GKGCMC	2 cut(s)	383, 1158
BanI	GGYRCC	2 cut(s)	688, 1153
BanII	GRGCYC	1 cut(s)	1238
BbsI	GAAGAC	5 cut(s)	106, 151, 331, 569, 980
Bbv12I	GWGCWC	1 cut(s)	8
BbvI	GCAGC	4 cut(s)	229, 267, 452, 466
BccI	CCATC	1 cut(s)	939
BceAI	ACGGC	6 cut(s)	95, 202, 268, 275, 478, 676
BciT130I	CCWGG	3 cut(s)	1041, 1302, 1318
BcnI	CCSGG	3 cut(s)	551, 596, 1105
BcoDI	GTCTC	5 cut(s)	18, 58, 497, 531, 1005
BfaI	CTAG	2 cut(s)	860, 993
BfoI	RGCGCY	1 cut(s)	692
BfuAI	ACCTGC	2 cut(s)	209, 413
BisI	GCNGC	9 cut(s)	83, 105, 108, 218, 281, 359, 422, 455, 466
BlsI	GCNGC	9 cut(s)	84, 106, 109, 219, 282, 360, 423, 456, 467
Bme1390I	CCNGG	6 cut(s)	551, 596, 1041, 1105, 1302, 1318
Bme18I	GGWCC	2 cut(s)	762, 964
BmeT110I	CYCGRG	2 cut(s)	500, 1247
BmgT120I	GGNCC	3 cut(s)	423, 762, 964
BmiI	GGNNCC	6 cut(s)	690, 763, 865, 966, 1155, 1235
BmrFI	CCNGG	6 cut(s)	551, 596, 1041, 1105, 1302, 1318
BmrI	ACTGGG	1 cut(s)	1099
BmsI	GCATC	3 cut(s)	486, 592, 1192
BmuI	ACTGGG	1 cut(s)	1099
BoxI	GACNNNNGTC	1 cut(s)	980
BpiI	GAAGAC	5 cut(s)	106, 151, 331, 569, 980
BpmI	CTGGAG	1 cut(s)	217
BpuMI	CCSGG	3 cut(s)	551, 596, 1105
BsaHI	GRCGYC	1 cut(s)	689
BsaJI	CCNNGG	3 cut(s)	460, 550, 612
BsaXI	ACNNNNNCTCC	2 cut(s)	368, 398
Bsc4I	CCNNNNNNNGG	2 cut(s)	309, 1010
Bse118I	RCCGGY	1 cut(s)	836
Bse1I	ACTGG	1 cut(s)	1094
BseBI	CCWGG	3 cut(s)	1041, 1302, 1318
BseDI	CCNNGG	3 cut(s)	460, 550, 612
BseGI	GGATG	7 cut(s)	162, 387, 528, 591, 604, 837, 1207
BseLI	CCNNNNNNNGG	2 cut(s)	309, 1010
BseMII	CTCAG	3 cut(s)	38, 252, 642
BseNI	ACTGG	1 cut(s)	1094
BseRI	GAGGAG	6 cut(s)	79, 82, 85, 88, 143, 967
BseSI	GKGCMC	2 cut(s)	383, 1158
BseX3I	CGGCCG	1 cut(s)	356
BseXI	GCAGC	4 cut(s)	229, 267, 452, 466
BseYI	CCCAGC	1 cut(s)	476
Bsh1236I	CGCG	2 cut(s)	637, 713
Bsh1285I	CGRYCG	1 cut(s)	359
BshFI	GGCC	4 cut(s)	213, 358, 424, 1305
BshNI	GGYRCC	2 cut(s)	688, 1153
BsiEI	CGRYCG	1 cut(s)	359
BsiHKAI	GWGCWC	1 cut(s)	8
BsiHKCI	CYCGRG	2 cut(s)	500, 1247
BsiSI	CCGG	4 cut(s)	550, 596, 837, 1105
BslI	CCNNNNNNNGG	2 cut(s)	309, 1010
BsmAI	GTCTC	5 cut(s)	18, 58, 497, 531, 1005
BsmBI	CGTCTC	1 cut(s)	497
BsnI	GGCC	4 cut(s)	213, 358, 424, 1305
BsoBI	CYCGRG	2 cut(s)	500, 1247
Bsp1286I	GDGCHC	4 cut(s)	8, 383, 1158, 1238
Bsp143I	GATC	4 cut(s)	27, 193, 469, 1078
Bsp19I	CCATGG	1 cut(s)	612
Bsp68I	TCGCGA	1 cut(s)	637
BspANI	GGCC	4 cut(s)	213, 358, 424, 1305
BspCNI	CTCAG	3 cut(s)	37, 251, 641
BspFNI	CGCG	2 cut(s)	637, 713
BspLI	GGNNCC	6 cut(s)	690, 763, 865, 966, 1155, 1235
BspMI	ACCTGC	2 cut(s)	209, 413
BspPI	GGATC	2 cut(s)	201, 1073
BspT107I	GGYRCC	2 cut(s)	688, 1153
BsrBI	CCGCTC	1 cut(s)	85
BsrFI	RCCGGY	1 cut(s)	836
BsrI	ACTGG	1 cut(s)	1094
BssAI	RCCGGY	1 cut(s)	836
BssECI	CCNNGG	3 cut(s)	460, 550, 612
BssMI	GATC	4 cut(s)	27, 193, 469, 1078
BssNI	GRCGYC	1 cut(s)	689
BssT1I	CCWWGG	1 cut(s)	612
Bst2UI	CCWGG	3 cut(s)	1041, 1302, 1318
Bst4CI	ACNGT	4 cut(s)	399, 643, 1052, 1153
Bst6I	CTCTTC	4 cut(s)	21, 149, 173, 263
BstACI	GRCGYC	1 cut(s)	689
BstC8I	GCNNGC	1 cut(s)	215
BstDEI	CTNAG	3 cut(s)	24, 238, 628
BstDSI	CCRYGG	2 cut(s)	460, 612
BstF5I	GGATG	7 cut(s)	162, 387, 528, 591, 604, 837, 1207
BstFNI	CGCG	2 cut(s)	637, 713
BstH2I	RGCGCY	1 cut(s)	692
BstHHI	GCGC	1 cut(s)	691
BstKTI	GATC	4 cut(s)	30, 196, 472, 1081
BstMAI	GTCTC	5 cut(s)	18, 58, 497, 531, 1005
BstMBI	GATC	4 cut(s)	27, 193, 469, 1078
BstMCI	CGRYCG	1 cut(s)	359
BstMWI	GCNNNNNNNGC	5 cut(s)	82, 210, 454, 505, 719
BstNI	CCWGG	3 cut(s)	1041, 1302, 1318
BstNSI	RCATGY	1 cut(s)	1000
BstPAI	GACNNNNGTC	1 cut(s)	980
BstSCI	CCNGG	6 cut(s)	549, 594, 1039, 1103, 1300, 1316
BstSLI	GKGCMC	2 cut(s)	383, 1158
BstUI	CGCG	2 cut(s)	637, 713
BstV1I	GCAGC	4 cut(s)	229, 267, 452, 466
BstV2I	GAAGAC	5 cut(s)	106, 151, 331, 569, 980
BstX2I	RGATCY	2 cut(s)	193, 1078
BstXI	CCANNNNNNTGG	2 cut(s)	613, 1089
BstYI	RGATCY	2 cut(s)	193, 1078
BstZI	CGGCCG	1 cut(s)	356
BsuRI	GGCC	4 cut(s)	213, 358, 424, 1305
BtgI	CCRYGG	2 cut(s)	460, 612
BtgZI	GCGATG	1 cut(s)	941
BtsCI	GGATG	7 cut(s)	162, 387, 528, 591, 604, 837, 1207
BtsI	GCAGTG	1 cut(s)	1235
BtsIMutI	CAGTG	1 cut(s)	1235
BtuMI	TCGCGA	1 cut(s)	637
BveI	ACCTGC	2 cut(s)	209, 413
Cac8I	GCNNGC	1 cut(s)	215
CfoI	GCGC	1 cut(s)	691
Cfr10I	RCCGGY	1 cut(s)	836
Cfr13I	GGNCC	3 cut(s)	423, 762, 964
CseI	GACGC	2 cut(s)	166, 514
Csp6I	GTAC	2 cut(s)	385, 1286
CviAII	CATG	7 cut(s)	61, 568, 613, 776, 961, 997, 1010
CviQI	GTAC	2 cut(s)	385, 1286
DdeI	CTNAG	3 cut(s)	24, 238, 628
DinI	GGCGCC	1 cut(s)	690
DpnI	GATC	4 cut(s)	29, 195, 471, 1080
DpnII	GATC	4 cut(s)	27, 193, 469, 1078
DraI	TTTAAA	1 cut(s)	850
DrdI	GACNNNNNNGTC	1 cut(s)	248
DseDI	GACNNNNNNGTC	1 cut(s)	248
EaeI	YGGCCR	2 cut(s)	211, 356
EagI	CGGCCG	1 cut(s)	356
Eam1104I	CTCTTC	4 cut(s)	21, 149, 173, 263
EarI	CTCTTC	4 cut(s)	21, 149, 173, 263
EclXI	CGGCCG	1 cut(s)	356
Eco130I	CCWWGG	1 cut(s)	612
Eco24I	GRGCYC	1 cut(s)	1238
Eco32I	GATATC	1 cut(s)	738
Eco47I	GGWCC	2 cut(s)	762, 964
Eco52I	CGGCCG	1 cut(s)	356
Eco88I	CYCGRG	2 cut(s)	500, 1247
EcoRII	CCWGG	3 cut(s)	1039, 1300, 1316
EcoRV	GATATC	1 cut(s)	738
EcoT14I	CCWWGG	1 cut(s)	612
EcoT38I	GRGCYC	1 cut(s)	1238
EgeI	GGCGCC	1 cut(s)	690
EheI	GGCGCC	1 cut(s)	690
ErhI	CCWWGG	1 cut(s)	612
Esp3I	CGTCTC	1 cut(s)	497
FaeI	CATG	7 cut(s)	64, 571, 616, 779, 964, 1000, 1013
FatI	CATG	7 cut(s)	60, 567, 612, 775, 960, 996, 1009
FauI	CCCGC	1 cut(s)	320
Fnu4HI	GCNGC	9 cut(s)	83, 105, 108, 218, 281, 359, 422, 455, 466
FokI	GGATG	7 cut(s)	149, 374, 535, 578, 611, 844, 1214
FriOI	GRGCYC	1 cut(s)	1238
Fsp4HI	GCNGC	9 cut(s)	83, 105, 108, 218, 281, 359, 422, 455, 466
FspBI	CTAG	2 cut(s)	860, 993
GlaI	GCGC	1 cut(s)	690
GluI	GCNGC	9 cut(s)	83, 105, 108, 218, 281, 359, 422, 455, 466
GsaI	CCCAGC	1 cut(s)	480
GsuI	CTGGAG	1 cut(s)	217
HaeII	RGCGCY	1 cut(s)	692
HaeIII	GGCC	4 cut(s)	213, 358, 424, 1305
HapII	CCGG	4 cut(s)	550, 596, 837, 1105
HgaI	GACGC	2 cut(s)	166, 514
HhaI	GCGC	1 cut(s)	691
Hin1I	GRCGYC	1 cut(s)	689
Hin1II	CATG	7 cut(s)	64, 571, 616, 779, 964, 1000, 1013
Hin6I	GCGC	1 cut(s)	689
HinP1I	GCGC	1 cut(s)	689
HincII	GTYRAC	2 cut(s)	351, 402
HindII	GTYRAC	2 cut(s)	351, 402
HinfI	GANTC	9 cut(s)	129, 347, 371, 539, 626, 632, 704, 1100, 1243
HpaII	CCGG	4 cut(s)	550, 596, 837, 1105
HphI	GGTGA	5 cut(s)	190, 220, 892, 961, 1046
Hpy166II	GTNNAC	3 cut(s)	351, 402, 1037
Hpy188I	TCNGA	9 cut(s)	27, 241, 257, 376, 546, 631, 742, 1099, 1242
Hpy188III	TCNNGA	5 cut(s)	502, 636, 701, 1247, 1310
Hpy8I	GTNNAC	3 cut(s)	351, 402, 1037
Hpy99I	CGWCG	4 cut(s)	296, 308, 347, 1153
HpyAV	CCTTC	6 cut(s)	45, 302, 784, 877, 1014, 1316
HpyCH4III	ACNGT	4 cut(s)	399, 643, 1052, 1153
HpyCH4V	TGCA	2 cut(s)	204, 1205
HpyF10VI	GCNNNNNNNGC	5 cut(s)	82, 210, 454, 505, 719
HpyF3I	CTNAG	3 cut(s)	24, 238, 628
Hsp92I	GRCGYC	1 cut(s)	689
Hsp92II	CATG	7 cut(s)	64, 571, 616, 779, 964, 1000, 1013
HspAI	GCGC	1 cut(s)	689
KasI	GGCGCC	1 cut(s)	688
Kzo9I	GATC	4 cut(s)	27, 193, 469, 1078
LmnI	GCTCC	3 cut(s)	11, 225, 1233
Lsp1109I	GCAGC	4 cut(s)	229, 267, 452, 466
LweI	GCATC	3 cut(s)	486, 592, 1192
MaeI	CTAG	2 cut(s)	860, 993
MaeIII	GTNAC	2 cut(s)	314, 1052
MalI	GATC	4 cut(s)	29, 195, 471, 1080
MbiI	CCGCTC	1 cut(s)	85
MboI	GATC	4 cut(s)	27, 193, 469, 1078
MflI	RGATCY	2 cut(s)	193, 1078
MhlI	GDGCHC	4 cut(s)	8, 383, 1158, 1238
MluCI	AATT	4 cut(s)	45, 845, 1125, 1253
Mly113I	GGCGCC	1 cut(s)	689
MlyI	GAGTC	6 cut(s)	123, 356, 533, 620, 626, 1237
MmeI	TCCRAC	2 cut(s)	247, 514
MseI	TTAA	2 cut(s)	849, 941
MslI	CAYNNNNRTG	2 cut(s)	494, 995
MspA1I	CMGCKG	1 cut(s)	327
MspI	CCGG	4 cut(s)	550, 596, 837, 1105
MspR9I	CCNGG	6 cut(s)	551, 596, 1041, 1105, 1302, 1318
MvaI	CCWGG	3 cut(s)	1041, 1302, 1318
MvnI	CGCG	2 cut(s)	637, 713
MwoI	GCNNNNNNNGC	5 cut(s)	82, 210, 454, 505, 719
NarI	GGCGCC	1 cut(s)	689
NciI	CCSGG	3 cut(s)	551, 596, 1105
NcoI	CCATGG	1 cut(s)	612
NdeII	GATC	4 cut(s)	27, 193, 469, 1078
NlaIII	CATG	7 cut(s)	64, 571, 616, 779, 964, 1000, 1013
NlaIV	GGNNCC	6 cut(s)	690, 763, 865, 966, 1155, 1235
NmeAIII	GCCGAG	1 cut(s)	334
NmuCI	GTSAC	1 cut(s)	314
NruI	TCGCGA	1 cut(s)	637
NspI	RCATGY	1 cut(s)	1000
PaeR7I	CTCGAG	1 cut(s)	500
PaqCI	CACCTGC	1 cut(s)	209
PcsI	WCGNNNNNNNCGW	3 cut(s)	300, 380, 423
PfeI	GAWTC	3 cut(s)	371, 704, 1100
PfoI	TCCNGGA	2 cut(s)	594, 1103
PkrI	GCNGC	9 cut(s)	84, 106, 109, 219, 282, 360, 423, 456, 467
PleI	GAGTC	6 cut(s)	123, 355, 533, 620, 626, 1237
PluTI	GGCGCC	1 cut(s)	692
PpsI	GAGTC	6 cut(s)	123, 355, 533, 620, 626, 1237
PshAI	GACNNNNGTC	1 cut(s)	980
Psp6I	CCWGG	3 cut(s)	1039, 1300, 1316
PspFI	CCCAGC	1 cut(s)	476
PspGI	CCWGG	3 cut(s)	1039, 1300, 1316
PspN4I	GGNNCC	6 cut(s)	690, 763, 865, 966, 1155, 1235
PspPI	GGNCC	3 cut(s)	423, 762, 964
PsuI	RGATCY	2 cut(s)	193, 1078
RruI	TCGCGA	1 cut(s)	637
RsaI	GTAC	2 cut(s)	386, 1287
RsaNI	GTAC	2 cut(s)	385, 1286
RseI	CAYNNNNRTG	2 cut(s)	494, 995
SaqAI	TTAA	2 cut(s)	849, 941
SatI	GCNGC	9 cut(s)	83, 105, 108, 218, 281, 359, 422, 455, 466
Sau3AI	GATC	4 cut(s)	27, 193, 469, 1078
Sau96I	GGNCC	3 cut(s)	423, 762, 964
SchI	GAGTC	6 cut(s)	123, 356, 533, 620, 626, 1237
ScrFI	CCNGG	6 cut(s)	551, 596, 1041, 1105, 1302, 1318
SduI	GDGCHC	4 cut(s)	8, 383, 1158, 1238
SfaNI	GCATC	3 cut(s)	486, 592, 1192
SfoI	GGCGCC	1 cut(s)	690
Sfr274I	CTCGAG	1 cut(s)	500
SinI	GGWCC	2 cut(s)	762, 964
SlaI	CTCGAG	1 cut(s)	500
SmiMI	CAYNNNNRTG	2 cut(s)	494, 995
SmlI	CTYRAG	1 cut(s)	500
SmoI	CTYRAG	1 cut(s)	500
Sse9I	AATT	4 cut(s)	45, 845, 1125, 1253
SspDI	GGCGCC	1 cut(s)	688
SspI	AATATT	1 cut(s)	855
SspMI	CTAG	2 cut(s)	860, 993
StyD4I	CCNGG	6 cut(s)	549, 594, 1039, 1103, 1300, 1316
StyI	CCWWGG	1 cut(s)	612
TaaI	ACNGT	4 cut(s)	399, 643, 1052, 1153
TaqI	TCGA	4 cut(s)	127, 342, 501, 675
TaqII	GACCGA	1 cut(s)	317
TasI	AATT	4 cut(s)	45, 845, 1125, 1253
TauI	GCSGC	5 cut(s)	85, 107, 110, 361, 424
TfiI	GAWTC	3 cut(s)	371, 704, 1100
Tru1I	TTAA	2 cut(s)	849, 941
Tru9I	TTAA	2 cut(s)	849, 941
TscAI	CASTG	1 cut(s)	1235
TseFI	GTSAC	1 cut(s)	314
TseI	GCWGC	4 cut(s)	217, 280, 454, 465
Tsp45I	GTSAC	1 cut(s)	314
TspDTI	ATGAA	3 cut(s)	86, 151, 944
TspGWI	ACGGA	1 cut(s)	754
TspRI	CASTG	1 cut(s)	1235
VpaK11BI	GGWCC	2 cut(s)	762, 964
XapI	RAATTY	1 cut(s)	845
XceI	RCATGY	1 cut(s)	1000
XhoI	CTCGAG	1 cut(s)	500
XspI	CTAG	2 cut(s)	860, 993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.