Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0014283

Physical Location & Seq

Genomic Coordinates Forward (+)

60914316 .. 60917398

Gene Structure 3083 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G032670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGTGTAATTATTTAGCTCACATTCATACAAAATTCTATTTCTACATGCACAAAATGAAGTCACCCCCATTCTCTTCCTCTTCTTCCTTCTCCGTTTTGCGTAAAGCTCGCCTCTCTCCCTACCTCTTCACCCTCCTCGCCTTCATTCTCTTCGTCGCCGTCCTCTACGGCGAGGACCTCATGTGCATTTTCAGCGAGCAACTCGAACCCCCCGCCGCCGCCTTCTCCGACCATCTCGTCGTCAAACCCGGGAAGATCAAGAGGCAGAAGCTGCCGTTCTCCATCGGGAAGACTGATCCGGAGTGCGACATCTTCACCGGAAGATGGGTTTGGGACGAGTCGAATCGGCCCCTTTACGAGGAATCGGAGTGTCCGTACATCCAGCCACAGCTGACTTGCCAGGAACACGGACGGCCGGATAAGGACTACCAGAAATGGCGGTGGCAGCCGGACGGCTGTGATCTTCCCAGCTTCAATGCTACTTTAATGCTGGAGACTCTACGAGGGAAGCGGATGATGTTTGTGGGAGATTCTCTGAACAGGGGTCAGTATGTTTCTATGATTTCCAAATCCATGGAAACCTTCGACTCAGATTCACGAACTGTTTTCACAGCCAAGGATTACAATGCCACAATTGAGTTCTATTGGGCGCCATTTCTTCTTGAGTCGAACTCGGATAATGCTGTCAACCATAGGATATCGGATAGGCTTGTCAGGAAAGGGTCCATGAATTACCATGGCAAACACTGGAAGGGGGTTGATGTATTGGTGTTCAATACCTATCTGTGGTGGATGACCGGCCAGAAATTTAAAATCTTGCAAGGGTCTTTTGAAGATGAGGTGAAAGATATTGTGGAGGTGCCAACCGCGGAGGCTTATCGTATGGCAATGAAAAGTATGCTAAGATGGGTGCGGAGGAACATGGACCGCAACAAGACAAGGGTCTTTTTCACAAGCATGTCACCTTCCCATGGAAAGAGTATTGACTGGGGAGGTGAACCAAACAGGAACTGTTACAATCAAACTAGTTTGATCGAAGATCCGAACTATTGGGGTTCAGATTCCCGGAAAAATATAATGGAGGTAATAGGAGAAGAATTCAGGAAGTCAAAGGTGCCCATTTCATTCCTCAACATCACCCAGCTCTCGAGTTATCGCAAAGATGCACACACGTCAATCTACAAGAAGCAGTGGAGTCCATTGACTCCAGAGCAATTAGCAAACCCTGTTAGCTATGCAGATTGTGTTCATTGGTGTTTGCCTGGTCTGCAGGATACTTGGAATGAGCTTCTCTTTGCCAAAATGTTCTATCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009832 GO:0009834 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044036 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1990538 GO:1990937

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

438

Amino Acids

51.2

Weight (kDa)

8.55

Isoelectric Point (pI)

57.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	101 - 154	8.7e-24	PMR5 N terminal Domain
PC-Esterase	PF13839	155 - 433	3.9e-81	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014283)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40320
fragaria_vesca	FvH4_7g25160 FvH4_7g25160
malus_domestica	MD01G1158300.v1.1 MD07G1227000.v1.1
prunus_persica	Prupe.2G256600_v2.0.a1
pyrus_communis	pycom01g17500 pycom07g20560
rosa_chinensis	RchiOBHm_Chr1g0371061
rosa_laevigata	RLG00000026989
rosa_multiflora	Rmu_sc0001018.1_g000029
rosa_roxburghii	Rroxscaffold_4G00286010 Rroxscaffold_4G00286020
rosa_rugosa	Rorug01G0361200
rosa_samantha	Rh1CG348200 Rh1DG366100
rosa_wichuraiana	Rw1G032670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	236
AccB1I	GGYRCC	3 cut(s)	649, 859, 1114
AccII	CGCG	1 cut(s)	869
AccIII	TCCGGA	1 cut(s)	298
AciI	CCGC	9 cut(s)	213, 216, 219, 439, 511, 867, 869, 913, 928
AclWI	GGATC	2 cut(s)	290, 1034
AcoI	YGGCCR	2 cut(s)	413, 799
AcsI	RAATTY	3 cut(s)	32, 806, 1097
AcyI	GRCGYC	1 cut(s)	650
AfaI	GTAC	1 cut(s)	377
AfiI	CCNNNNNNNGG	4 cut(s)	324, 358, 786, 971
AflIII	ACRYGT	1 cut(s)	1170
AgsI	TTSAA	3 cut(s)	475, 775, 833
AhlI	ACTAGT	1 cut(s)	1025
AjiI	CACGTC	1 cut(s)	1173
AjnI	CCWGG	2 cut(s)	399, 1261
AjuI	GAANNNNNNNTTGG	2 cut(s)	313, 345
AluBI	AGCT	8 cut(s)	17, 107, 271, 391, 471, 1144, 1233, 1288
AluI	AGCT	8 cut(s)	17, 107, 271, 391, 471, 1144, 1233, 1288
Alw26I	GTCTC	1 cut(s)	488
AlwI	GGATC	2 cut(s)	290, 1034
AlwNI	CAGNNNCTG	2 cut(s)	271, 1011
Ama87I	CYCGRG	2 cut(s)	248, 1147
Aor13HI	TCCGGA	1 cut(s)	298
AoxI	GGCC	3 cut(s)	347, 413, 799
ApeKI	GCWGC	2 cut(s)	271, 445
ApoI	RAATTY	3 cut(s)	32, 806, 1097
AspLEI	GCGC	1 cut(s)	652
AspS9I	GGNCC	4 cut(s)	175, 348, 723, 925
AsuC2I	CCSGG	3 cut(s)	249, 250, 1066
AsuHPI	GGTGA	7 cut(s)	54, 121, 307, 853, 954, 1007, 1129
AvaI	CYCGRG	2 cut(s)	248, 1147
AvaII	GGWCC	3 cut(s)	175, 723, 925
BaeGI	GKGCMC	1 cut(s)	1119
BanI	GGYRCC	3 cut(s)	649, 859, 1114
BbsI	GAAGAC	1 cut(s)	296
BbvI	GCAGC	2 cut(s)	258, 457
BccI	CCATC	4 cut(s)	240, 290, 318, 900
BceAI	ACGGC	5 cut(s)	143, 184, 259, 428, 469
BciT130I	CCWGG	2 cut(s)	401, 1263
BciVI	GTATCC	1 cut(s)	1267
BcnI	CCSGG	3 cut(s)	249, 250, 1066
BcoDI	GTCTC	1 cut(s)	488
BcuI	ACTAGT	1 cut(s)	1025
BfaI	CTAG	1 cut(s)	1026
BfmI	CTRYAG	1 cut(s)	1268
BfoI	RGCGCY	1 cut(s)	653
BfuI	GTATCC	1 cut(s)	1267
BisI	GCNGC	4 cut(s)	216, 219, 272, 446
BlsI	GCNGC	4 cut(s)	217, 220, 273, 447
Bme1390I	CCNGG	5 cut(s)	249, 250, 401, 1066, 1263
Bme18I	GGWCC	3 cut(s)	175, 723, 925
BmeT110I	CYCGRG	2 cut(s)	248, 1147
BmgBI	CACGTC	1 cut(s)	1173
BmgT120I	GGNCC	4 cut(s)	175, 348, 723, 925
BmiI	GGNNCC	5 cut(s)	350, 651, 724, 861, 1116
BmrFI	CCNGG	5 cut(s)	249, 250, 401, 1066, 1263
BmrI	ACTGGG	1 cut(s)	997
BmsI	GCATC	1 cut(s)	1153
BmuI	ACTGGG	1 cut(s)	997
BoxI	GACNNNNGTC	1 cut(s)	941
BpiI	GAAGAC	1 cut(s)	296
BplI	GAGNNNNNCTC	2 cut(s)	656, 688
BpmI	CTGGAG	2 cut(s)	512, 1191
BpuEI	CTTGAG	1 cut(s)	683
BpuMI	CCSGG	3 cut(s)	249, 250, 1066
BsaHI	GRCGYC	1 cut(s)	650
BsaJI	CCNNGG	6 cut(s)	248, 573, 615, 736, 867, 970
BsaWI	WCCGGW	2 cut(s)	298, 317
BsaXI	ACNNNNNCTCC	2 cut(s)	359, 389
Bsc4I	CCNNNNNNNGG	4 cut(s)	324, 358, 786, 971
Bse118I	RCCGGY	1 cut(s)	797
Bse1I	ACTGG	2 cut(s)	752, 992
Bse3DI	GCAATG	1 cut(s)	894
BseAI	TCCGGA	1 cut(s)	298
BseBI	CCWGG	2 cut(s)	401, 1263
BseDI	CCNNGG	6 cut(s)	248, 573, 615, 736, 867, 970
BseGI	GGATG	3 cut(s)	378, 519, 798
BseLI	CCNNNNNNNGG	4 cut(s)	324, 358, 786, 971
BseMI	GCAATG	1 cut(s)	894
BseMII	CTCAG	1 cut(s)	603
BseNI	ACTGG	2 cut(s)	752, 992
BseRI	GAGGAG	1 cut(s)	125
BseSI	GKGCMC	1 cut(s)	1119
BseX3I	CGGCCG	1 cut(s)	413
BseXI	GCAGC	2 cut(s)	258, 457
BseYI	CCCAGC	2 cut(s)	467, 1140
Bsh1236I	CGCG	1 cut(s)	869
Bsh1285I	CGRYCG	1 cut(s)	416
BshFI	GGCC	3 cut(s)	349, 415, 801
BshNI	GGYRCC	3 cut(s)	649, 859, 1114
BsiEI	CGRYCG	1 cut(s)	416
BsiHKCI	CYCGRG	2 cut(s)	248, 1147
BsiSI	CCGG	7 cut(s)	249, 299, 318, 416, 449, 798, 1066
BslFI	GGGAC	1 cut(s)	347
BslI	CCNNNNNNNGG	4 cut(s)	324, 358, 786, 971
BsmAI	GTCTC	1 cut(s)	488
BsmFI	GGGAC	1 cut(s)	347
BsnI	GGCC	3 cut(s)	349, 415, 801
BsoBI	CYCGRG	2 cut(s)	248, 1147
Bsp1286I	GDGCHC	1 cut(s)	1119
Bsp13I	TCCGGA	1 cut(s)	298
Bsp143I	GATC	5 cut(s)	255, 295, 460, 1032, 1039
Bsp19I	CCATGG	3 cut(s)	573, 736, 970
BspACI	CCGC	9 cut(s)	213, 216, 219, 439, 511, 867, 869, 913, 928
BspANI	GGCC	3 cut(s)	349, 415, 801
BspCNI	CTCAG	1 cut(s)	602
BspEI	TCCGGA	1 cut(s)	298
BspFNI	CGCG	1 cut(s)	869
BspLI	GGNNCC	5 cut(s)	350, 651, 724, 861, 1116
BspMAI	CTGCAG	1 cut(s)	1272
BspPI	GGATC	2 cut(s)	290, 1034
BspT107I	GGYRCC	3 cut(s)	649, 859, 1114
BsrDI	GCAATG	1 cut(s)	894
BsrFI	RCCGGY	1 cut(s)	797
BsrI	ACTGG	2 cut(s)	752, 992
BssAI	RCCGGY	1 cut(s)	797
BssECI	CCNNGG	6 cut(s)	248, 573, 615, 736, 867, 970
BssMI	GATC	5 cut(s)	255, 295, 460, 1032, 1039
BssNI	GRCGYC	1 cut(s)	650
BssT1I	CCWWGG	4 cut(s)	573, 615, 736, 970
Bst2UI	CCWGG	2 cut(s)	401, 1263
Bst4CI	ACNGT	2 cut(s)	604, 1013
Bst6I	CTCTTC	4 cut(s)	79, 85, 131, 155
BstACI	GRCGYC	1 cut(s)	650
BstAPI	GCANNNNNTGC	1 cut(s)	271
BstC8I	GCNNGC	2 cut(s)	109, 197
BstDEI	CTNAG	2 cut(s)	589, 902
BstDSI	CCRYGG	4 cut(s)	573, 736, 867, 970
BstENI	CCTNNNNNAGG	1 cut(s)	356
BstF5I	GGATG	3 cut(s)	378, 519, 798
BstFNI	CGCG	1 cut(s)	869
BstH2I	RGCGCY	1 cut(s)	653
BstHHI	GCGC	1 cut(s)	652
BstKTI	GATC	5 cut(s)	258, 298, 463, 1035, 1042
BstMAI	GTCTC	1 cut(s)	488
BstMBI	GATC	5 cut(s)	255, 295, 460, 1032, 1039
BstMCI	CGRYCG	1 cut(s)	416
BstMWI	GCNNNNNNNGC	4 cut(s)	192, 271, 445, 1267
BstNI	CCWGG	2 cut(s)	401, 1263
BstNSI	RCATGY	2 cut(s)	49, 961
BstPAI	GACNNNNGTC	1 cut(s)	941
BstSCI	CCNGG	5 cut(s)	247, 248, 399, 1064, 1261
BstSFI	CTRYAG	1 cut(s)	1268
BstSLI	GKGCMC	1 cut(s)	1119
BstUI	CGCG	1 cut(s)	869
BstV1I	GCAGC	2 cut(s)	258, 457
BstV2I	GAAGAC	1 cut(s)	296
BstX2I	RGATCY	1 cut(s)	1039
BstXI	CCANNNNNNTGG	1 cut(s)	574
BstYI	RGATCY	1 cut(s)	1039
BstZI	CGGCCG	1 cut(s)	413
BsuI	GTATCC	1 cut(s)	1267
BsuRI	GGCC	3 cut(s)	349, 415, 801
BtgI	CCRYGG	4 cut(s)	573, 736, 867, 970
BtrI	CACGTC	1 cut(s)	1173
BtsCI	GGATG	3 cut(s)	378, 519, 798
BtsI	GCAGTG	1 cut(s)	1196
BtsIMutI	CAGTG	2 cut(s)	745, 1196
Cac8I	GCNNGC	2 cut(s)	109, 197
CaiI	CAGNNNCTG	2 cut(s)	271, 1011
CfoI	GCGC	1 cut(s)	652
Cfr10I	RCCGGY	1 cut(s)	797
Cfr13I	GGNCC	4 cut(s)	175, 348, 723, 925
Cfr42I	CCGCGG	1 cut(s)	870
Cfr9I	CCCGGG	1 cut(s)	248
Csp6I	GTAC	1 cut(s)	376
CviAII	CATG	8 cut(s)	46, 181, 574, 727, 737, 922, 958, 971
CviQI	GTAC	1 cut(s)	376
DdeI	CTNAG	2 cut(s)	589, 902
DinI	GGCGCC	1 cut(s)	651
DpnI	GATC	5 cut(s)	257, 297, 462, 1034, 1041
DpnII	GATC	5 cut(s)	255, 295, 460, 1032, 1039
DraI	TTTAAA	1 cut(s)	811
DrdI	GACNNNNNNGTC	1 cut(s)	236
DseDI	GACNNNNNNGTC	1 cut(s)	236
EaeI	YGGCCR	2 cut(s)	413, 799
EagI	CGGCCG	1 cut(s)	413
Eam1104I	CTCTTC	4 cut(s)	79, 85, 131, 155
EarI	CTCTTC	4 cut(s)	79, 85, 131, 155
EclXI	CGGCCG	1 cut(s)	413
Eco130I	CCWWGG	4 cut(s)	573, 615, 736, 970
Eco32I	GATATC	1 cut(s)	699
Eco47I	GGWCC	3 cut(s)	175, 723, 925
Eco52I	CGGCCG	1 cut(s)	413
Eco88I	CYCGRG	2 cut(s)	248, 1147
EcoNI	CCTNNNNNAGG	1 cut(s)	356
EcoO109I	RGGNCCY	1 cut(s)	175
EcoRI	GAATTC	1 cut(s)	1097
EcoRII	CCWGG	2 cut(s)	399, 1261
EcoRV	GATATC	1 cut(s)	699
EcoT14I	CCWWGG	4 cut(s)	573, 615, 736, 970
EgeI	GGCGCC	1 cut(s)	651
EheI	GGCGCC	1 cut(s)	651
ErhI	CCWWGG	4 cut(s)	573, 615, 736, 970
FaeI	CATG	8 cut(s)	49, 184, 577, 730, 740, 925, 961, 974
FaqI	GGGAC	1 cut(s)	347
FatI	CATG	8 cut(s)	45, 180, 573, 726, 736, 921, 957, 970
FauI	CCCGC	1 cut(s)	220
Fnu4HI	GCNGC	4 cut(s)	216, 219, 272, 446
FokI	GGATG	3 cut(s)	365, 526, 805
Fsp4HI	GCNGC	4 cut(s)	216, 219, 272, 446
FspBI	CTAG	1 cut(s)	1026
GlaI	GCGC	1 cut(s)	651
GluI	GCNGC	4 cut(s)	216, 219, 272, 446
GsaI	CCCAGC	2 cut(s)	471, 1144
GsuI	CTGGAG	2 cut(s)	512, 1191
HaeII	RGCGCY	1 cut(s)	653
HaeIII	GGCC	3 cut(s)	349, 415, 801
HapII	CCGG	7 cut(s)	249, 299, 318, 416, 449, 798, 1066
HhaI	GCGC	1 cut(s)	652
Hin1I	GRCGYC	1 cut(s)	650
Hin1II	CATG	8 cut(s)	49, 184, 577, 730, 740, 925, 961, 974
Hin6I	GCGC	1 cut(s)	650
HinP1I	GCGC	1 cut(s)	650
HincII	GTYRAC	1 cut(s)	688
HindII	GTYRAC	1 cut(s)	688
HpaII	CCGG	7 cut(s)	249, 299, 318, 416, 449, 798, 1066
HphI	GGTGA	7 cut(s)	54, 121, 307, 853, 954, 1007, 1129
Hpy166II	GTNNAC	2 cut(s)	688, 998
Hpy188I	TCNGA	8 cut(s)	229, 367, 537, 592, 676, 703, 1044, 1060
Hpy188III	TCNNGA	9 cut(s)	259, 286, 299, 597, 662, 715, 1102, 1147, 1208
Hpy8I	GTNNAC	2 cut(s)	688, 998
Hpy99I	CGWCG	2 cut(s)	158, 242
HpyAV	CCTTC	6 cut(s)	97, 151, 232, 592, 745, 975
HpyCH4III	ACNGT	2 cut(s)	604, 1013
HpyCH4IV	ACGT	1 cut(s)	1172
HpyCH4V	TGCA	6 cut(s)	49, 186, 820, 1166, 1238, 1270
HpyF10VI	GCNNNNNNNGC	4 cut(s)	192, 271, 445, 1267
HpyF3I	CTNAG	2 cut(s)	589, 902
HpySE526I	ACGT	1 cut(s)	1172
Hsp92I	GRCGYC	1 cut(s)	650
Hsp92II	CATG	8 cut(s)	49, 184, 577, 730, 740, 925, 961, 974
HspAI	GCGC	1 cut(s)	650
KasI	GGCGCC	1 cut(s)	649
Kpn2I	TCCGGA	1 cut(s)	298
KspI	CCGCGG	1 cut(s)	870
Kzo9I	GATC	5 cut(s)	255, 295, 460, 1032, 1039
Lsp1109I	GCAGC	2 cut(s)	258, 457
LweI	GCATC	1 cut(s)	1153
MaeI	CTAG	1 cut(s)	1026
MaeII	ACGT	1 cut(s)	1172
MaeIII	GTNAC	3 cut(s)	60, 960, 1013
MalI	GATC	5 cut(s)	257, 297, 462, 1034, 1041
MboI	GATC	5 cut(s)	255, 295, 460, 1032, 1039
MfeI	CAATTG	1 cut(s)	633
MflI	RGATCY	1 cut(s)	1039
MhlI	GDGCHC	1 cut(s)	1119
MluCI	AATT	7 cut(s)	7, 32, 633, 730, 806, 1097, 1214
Mly113I	GGCGCC	1 cut(s)	650
MlyI	GAGTC	6 cut(s)	347, 490, 581, 674, 1198, 1204
MmeI	TCCRAC	1 cut(s)	252
MroI	TCCGGA	1 cut(s)	298
MseI	TTAA	2 cut(s)	485, 810
MslI	CAYNNNNRTG	1 cut(s)	956
MspA1I	CMGCKG	2 cut(s)	391, 869
MspI	CCGG	7 cut(s)	249, 299, 318, 416, 449, 798, 1066
MspR9I	CCNGG	5 cut(s)	249, 250, 401, 1066, 1263
MunI	CAATTG	1 cut(s)	633
MvaI	CCWGG	2 cut(s)	401, 1263
MvnI	CGCG	1 cut(s)	869
MwoI	GCNNNNNNNGC	4 cut(s)	192, 271, 445, 1267
NarI	GGCGCC	1 cut(s)	650
NciI	CCSGG	3 cut(s)	249, 250, 1066
NcoI	CCATGG	3 cut(s)	573, 736, 970
NdeII	GATC	5 cut(s)	255, 295, 460, 1032, 1039
NlaIII	CATG	8 cut(s)	49, 184, 577, 730, 740, 925, 961, 974
NlaIV	GGNNCC	5 cut(s)	350, 651, 724, 861, 1116
NmuCI	GTSAC	2 cut(s)	60, 960
NspI	RCATGY	2 cut(s)	49, 961
PaeR7I	CTCGAG	1 cut(s)	1147
PcsI	WCGNNNNNNNCGW	1 cut(s)	371
PfeI	GAWTC	5 cut(s)	343, 362, 530, 593, 1061
PfoI	TCCNGGA	1 cut(s)	1064
PkrI	GCNGC	4 cut(s)	217, 220, 273, 447
PleI	GAGTC	6 cut(s)	346, 490, 581, 673, 1198, 1203
PluTI	GGCGCC	1 cut(s)	653
PpsI	GAGTC	6 cut(s)	346, 490, 581, 673, 1198, 1203
PpuMI	RGGWCCY	1 cut(s)	175
PshAI	GACNNNNGTC	1 cut(s)	941
Psp5II	RGGWCCY	1 cut(s)	175
Psp6I	CCWGG	2 cut(s)	399, 1261
PspFI	CCCAGC	2 cut(s)	467, 1140
PspGI	CCWGG	2 cut(s)	399, 1261
PspN4I	GGNNCC	5 cut(s)	350, 651, 724, 861, 1116
PspPI	GGNCC	4 cut(s)	175, 348, 723, 925
PspPPI	RGGWCCY	1 cut(s)	175
PstI	CTGCAG	1 cut(s)	1272
PstNI	CAGNNNCTG	2 cut(s)	271, 1011
PsuI	RGATCY	1 cut(s)	1039
PvuII	CAGCTG	1 cut(s)	391
RsaI	GTAC	1 cut(s)	377
RsaNI	GTAC	1 cut(s)	376
RseI	CAYNNNNRTG	1 cut(s)	956
SacII	CCGCGG	1 cut(s)	870
SaqAI	TTAA	2 cut(s)	485, 810
SatI	GCNGC	4 cut(s)	216, 219, 272, 446
Sau3AI	GATC	5 cut(s)	255, 295, 460, 1032, 1039
Sau96I	GGNCC	4 cut(s)	175, 348, 723, 925
SchI	GAGTC	6 cut(s)	347, 490, 581, 674, 1198, 1204
ScrFI	CCNGG	5 cut(s)	249, 250, 401, 1066, 1263
SduI	GDGCHC	1 cut(s)	1119
SfaNI	GCATC	1 cut(s)	1153
SfcI	CTRYAG	1 cut(s)	1268
SfoI	GGCGCC	1 cut(s)	651
Sfr274I	CTCGAG	1 cut(s)	1147
Sfr303I	CCGCGG	1 cut(s)	870
SgrBI	CCGCGG	1 cut(s)	870
SinI	GGWCC	3 cut(s)	175, 723, 925
SlaI	CTCGAG	1 cut(s)	1147
SmaI	CCCGGG	1 cut(s)	250
SmiMI	CAYNNNNRTG	1 cut(s)	956
SmlI	CTYRAG	2 cut(s)	662, 1147
SmoI	CTYRAG	2 cut(s)	662, 1147
SpeI	ACTAGT	1 cut(s)	1025
Sse9I	AATT	7 cut(s)	7, 32, 633, 730, 806, 1097, 1214
SsiI	CCGC	9 cut(s)	213, 216, 219, 439, 511, 867, 869, 913, 928
SspDI	GGCGCC	1 cut(s)	649
SspMI	CTAG	1 cut(s)	1026
StyD4I	CCNGG	5 cut(s)	247, 248, 399, 1064, 1261
StyI	CCWWGG	4 cut(s)	573, 615, 736, 970
TaaI	ACNGT	2 cut(s)	604, 1013
TaiI	ACGT	1 cut(s)	1175
TaqI	TCGA	6 cut(s)	204, 341, 585, 668, 1035, 1148
TasI	AATT	7 cut(s)	7, 32, 633, 730, 806, 1097, 1214
TauI	GCSGC	2 cut(s)	218, 221
TfiI	GAWTC	5 cut(s)	343, 362, 530, 593, 1061
Tru1I	TTAA	2 cut(s)	485, 810
Tru9I	TTAA	2 cut(s)	485, 810
TscAI	CASTG	2 cut(s)	752, 1196
TseFI	GTSAC	2 cut(s)	60, 960
TseI	GCWGC	2 cut(s)	271, 445
Tsp45I	GTSAC	2 cut(s)	60, 960
TspDTI	ATGAA	7 cut(s)	14, 71, 133, 743, 905, 1113, 1238
TspGWI	ACGGA	3 cut(s)	82, 363, 423
TspMI	CCCGGG	1 cut(s)	248
TspRI	CASTG	2 cut(s)	752, 1196
VpaK11BI	GGWCC	3 cut(s)	175, 723, 925
XagI	CCTNNNNNAGG	1 cut(s)	356
XapI	RAATTY	3 cut(s)	32, 806, 1097
XceI	RCATGY	2 cut(s)	49, 961
XhoI	CTCGAG	1 cut(s)	1147
XmaI	CCCGGG	1 cut(s)	248
XspI	CTAG	1 cut(s)	1026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.