MD07G1243700.v1.1

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006090
Physical Location & Seq
Forward (+)
31396695 .. 31398727
2033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1243700.v1.1.491

Sequence Viewer

Length: 867 bp
ATGGAAGCCCTGATTGCCTCGTACGGAGACTCGTCGTCGGGCTCAGACTCCGAGTCGCCGGCTCCACCTCCACCACCACCTCCGGAGCTCAACAATCCTCAAAAACCGACCCCTGCGCTGCCTCCACCTCCTCTGTCACTTCTTGACAGCCCCAATTCCTTCGGATTTCTGGACTTCTCGGCAAGCGCCCAGCCGAGCAGAGTTCGGAACTTTCCTCACGTCGAAGGGAACTACGCAGTACATGTATACATCCCAGTTTATATACCACCAGCACCAAGGAAAGAGATGGCCTTGTTTTTGAACAAGCTAGCTTCTCTGGTGCCTGGTCTCCATGCGGTTGACGTTGACTTCCCGCTTGACATTCTGCGTAAGGACGAGCACAAGCTTGAACAAGTTGCTTTAGGCAGAGAGTTCCATATAAGTCTCGGAAGAACTGTTCCGATTCGGGTGCACCAGATTGATTCCTTGGTGACAATGCTGCGGCAGAAGCTTCAGATTCAGAGGCGGTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAATGATGATCAGACCCGGACCTTTGTGTCAATCGAAATCATTGCTGCTGGGTTAGCTGAGATAACAAAGCAGATTCAAGCTGTGAATGAGGTGTATAAGCTTCACAATCTTCCTGAATTTTACAAGGATCCACGCCCTCACATCTCGGTAGCTTGGGCATTGGGTGACATCAGCAATTCCCTGAAGAAAGTGGTTGAAGAAGAAAGACGAAGATCTACCATCGGAGGATCGTTACAGAAATGTATTTTTACCAGTAAATTCAATGGTATCGAATGTAGGATTGGTAATGAGACACACAAAATATGTAAATTTTCTGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

289

Amino Acids

32.37

Weight (kDa)

6.52

Isoelectric Point (pI)

61.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 41 - 283 4.6e-56 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 246
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 4 cut(s) 335, 353, 481, 505
AclWI GGATC 3 cut(s) 665, 678, 778
AcsI RAATTY 3 cut(s) 659, 800, 851
AcuI CTGAAG 2 cut(s) 476, 746
AfaI GTAC 2 cut(s) 23, 240
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 5 cut(s) 301, 389, 620, 740, 805
AhdI GACNNNNNGTC 2 cut(s) 34, 52
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 1 cut(s) 322
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 9 cut(s) 88, 307, 311, 385, 490, 599, 623, 643, 695
AluI AGCT 9 cut(s) 88, 307, 311, 385, 490, 599, 623, 643, 695
Alw21I GWGCWC 3 cut(s) 90, 381, 453
Alw26I GTCTC 4 cut(s) 21, 332, 428, 827
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 3 cut(s) 665, 678, 778
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 1 cut(s) 288
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 3 cut(s) 118, 478, 587
ApoI RAATTY 3 cut(s) 659, 800, 851
AspLEI GCGC 2 cut(s) 118, 188
AspS9I GGNCC 1 cut(s) 561
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 481, 719
AsuNHI GCTAGC 1 cut(s) 307
AvaII GGWCC 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 453
BamHI GGATCC 1 cut(s) 670
BanI GGYRCC 1 cut(s) 319
BanII GRGCYC 2 cut(s) 44, 90
Bbv12I GWGCWC 3 cut(s) 90, 381, 453
BbvI GCAGC 3 cut(s) 105, 465, 574
BccI CCATC 2 cut(s) 280, 770
BcgI CGANNNNNNTGC 4 cut(s) 430, 464, 566, 600
BciT130I CCWGG 1 cut(s) 324
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 4 cut(s) 21, 332, 428, 827
BfaI CTAG 1 cut(s) 308
BfoI RGCGCY 1 cut(s) 189
BglII AGATCT 1 cut(s) 755
BisI GCNGC 4 cut(s) 119, 479, 482, 588
BlsI GCNGC 4 cut(s) 120, 480, 483, 589
Bme1390I CCNGG 2 cut(s) 324, 559
Bme18I GGWCC 1 cut(s) 561
BmeRI GACNNNNNGTC 2 cut(s) 34, 52
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 561
BmiI GGNNCC 3 cut(s) 63, 321, 672
BmrFI CCNGG 2 cut(s) 324, 559
BmrI ACTGGG 1 cut(s) 248
BmtI GCTAGC 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 248
BpuMI CCSGG 1 cut(s) 559
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 275, 465
BsaWI WCCGGW 1 cut(s) 82
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 254, 795
Bse3DI GCAATG 1 cut(s) 582
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 275, 465
BseGI GGATG 1 cut(s) 249
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 2 cut(s) 57, 591
BseNI ACTGG 2 cut(s) 254, 795
BseRI GAGGAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 453
BseXI GCAGC 3 cut(s) 105, 465, 574
BseYI CCCAGC 2 cut(s) 189, 590
BshFI GGCC 1 cut(s) 290
BshNI GGYRCC 1 cut(s) 319
BsiHKAI GWGCWC 3 cut(s) 90, 381, 453
BsiSI CCGG 3 cut(s) 59, 83, 559
BsiWI CGTACG 1 cut(s) 21
BsmAI GTCTC 4 cut(s) 21, 332, 428, 827
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 4 cut(s) 44, 90, 381, 453
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 4 cut(s) 550, 670, 755, 770
BspACI CCGC 4 cut(s) 335, 353, 481, 505
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 2 cut(s) 56, 592
BspEI TCCGGA 1 cut(s) 82
BspLI GGNNCC 3 cut(s) 63, 321, 672
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 3 cut(s) 665, 678, 778
BspT107I GGYRCC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 58
BsrI ACTGG 2 cut(s) 254, 795
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 275, 465
BssMI GATC 4 cut(s) 550, 670, 755, 770
BssNAI GTATAC 1 cut(s) 247
BssT1I CCWWGG 2 cut(s) 275, 465
Bst1107I GTATAC 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 436
BstC8I GCNNGC 3 cut(s) 60, 184, 309
BstDEI CTNAG 2 cut(s) 43, 600
BstF5I GGATG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 118, 188
BstKTI GATC 4 cut(s) 553, 673, 758, 773
BstMAI GTCTC 4 cut(s) 21, 332, 428, 827
BstMBI GATC 4 cut(s) 550, 670, 755, 770
BstMWI GCNNNNNNNGC 3 cut(s) 14, 487, 596
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 2 cut(s) 322, 557
BstSLI GKGCMC 1 cut(s) 453
BstV1I GCAGC 3 cut(s) 105, 465, 574
BstX2I RGATCY 2 cut(s) 670, 755
BstYI RGATCY 2 cut(s) 670, 755
BstZ17I GTATAC 1 cut(s) 247
BsuRI GGCC 1 cut(s) 290
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 249
Cac8I GCNNGC 3 cut(s) 60, 184, 309
CfoI GCGC 2 cut(s) 118, 188
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 1 cut(s) 561
Csp6I GTAC 2 cut(s) 22, 239
CviAII CATG 2 cut(s) 242, 332
CviQI GTAC 2 cut(s) 22, 239
DdeI CTNAG 2 cut(s) 43, 600
DpnI GATC 4 cut(s) 552, 672, 757, 772
DpnII GATC 4 cut(s) 550, 670, 755, 770
DrdI GACNNNNNNGTC 1 cut(s) 568
DriI GACNNNNNGTC 2 cut(s) 34, 52
DseDI GACNNNNNNGTC 1 cut(s) 568
Eam1105I GACNNNNNGTC 2 cut(s) 34, 52
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 2 cut(s) 275, 465
Eco24I GRGCYC 2 cut(s) 44, 90
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 1 cut(s) 561
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 476, 746
EcoICRI GAGCTC 1 cut(s) 88
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 275, 465
EcoT38I GRGCYC 2 cut(s) 44, 90
ErhI CCWWGG 2 cut(s) 275, 465
FaeI CATG 2 cut(s) 245, 335
FaiI YATR 9 cut(s) 243, 247, 261, 263, 333, 417, 419, 639, 847
FatI CATG 2 cut(s) 241, 331
FauI CCCGC 1 cut(s) 360
FbaI TGATCA 1 cut(s) 550
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 119, 479, 482, 588
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 2 cut(s) 44, 90
Fsp4HI GCNGC 4 cut(s) 119, 479, 482, 588
FspBI CTAG 1 cut(s) 308
GlaI GCGC 2 cut(s) 117, 187
GluI GCNGC 4 cut(s) 119, 479, 482, 588
GsaI CCCAGC 2 cut(s) 193, 594
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 1 cut(s) 290
HapII CCGG 3 cut(s) 59, 83, 559
HhaI GCGC 2 cut(s) 118, 188
Hin1II CATG 2 cut(s) 245, 335
Hin6I GCGC 2 cut(s) 116, 186
HinP1I GCGC 2 cut(s) 116, 186
HincII GTYRAC 2 cut(s) 340, 346
HindII GTYRAC 2 cut(s) 340, 346
HindIII AAGCTT 3 cut(s) 383, 488, 641
HinfI GANTC 7 cut(s) 29, 47, 53, 442, 461, 496, 616
HpaII CCGG 3 cut(s) 59, 83, 559
HphI GGTGA 2 cut(s) 481, 719
Hpy166II GTNNAC 4 cut(s) 247, 340, 346, 451
Hpy188III TCNNGA 4 cut(s) 83, 143, 170, 656
Hpy8I GTNNAC 4 cut(s) 247, 340, 346, 451
Hpy99I CGWCG 3 cut(s) 37, 40, 224
HpyAV CCTTC 2 cut(s) 169, 218
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 2 cut(s) 219, 342
HpyCH4V TGCA 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 487, 596
HpyF3I CTNAG 2 cut(s) 43, 600
HpySE526I ACGT 2 cut(s) 219, 342
Hsp92II CATG 2 cut(s) 245, 335
HspAI GCGC 2 cut(s) 116, 186
Kpn2I TCCGGA 1 cut(s) 82
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 550
Kzo9I GATC 4 cut(s) 550, 670, 755, 770
LmnI GCTCC 2 cut(s) 67, 85
Lsp1109I GCAGC 3 cut(s) 105, 465, 574
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 219, 342
MaeIII GTNAC 4 cut(s) 135, 469, 707, 774
MalI GATC 4 cut(s) 552, 672, 757, 772
MboI GATC 4 cut(s) 550, 670, 755, 770
MboII GAAGA 6 cut(s) 441, 644, 739, 752, 755, 765
MflI RGATCY 2 cut(s) 670, 755
MhlI GDGCHC 4 cut(s) 44, 90, 381, 453
MluCI AATT 5 cut(s) 154, 659, 718, 800, 851
MlyI GAGTC 3 cut(s) 23, 41, 62
MroI TCCGGA 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 58
MseI TTAA 1 cut(s) 543
MspI CCGG 3 cut(s) 59, 83, 559
MspR9I CCNGG 2 cut(s) 324, 559
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 3 cut(s) 14, 487, 596
NaeI GCCGGC 1 cut(s) 60
NciI CCSGG 1 cut(s) 559
NdeII GATC 4 cut(s) 550, 670, 755, 770
NgoMIV GCCGGC 1 cut(s) 58
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 245, 335
NlaIV GGNNCC 3 cut(s) 63, 321, 672
NmeAIII GCCGAG 2 cut(s) 158, 219
NmuCI GTSAC 3 cut(s) 135, 469, 707
NspI RCATGY 1 cut(s) 245
PciI ACATGT 1 cut(s) 241
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 4 cut(s) 442, 461, 496, 616
Pfl23II CGTACG 1 cut(s) 21
PkrI GCNGC 4 cut(s) 120, 480, 483, 589
PleI GAGTC 3 cut(s) 23, 41, 61
PpsI GAGTC 3 cut(s) 23, 41, 61
PscI ACATGT 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 2 cut(s) 189, 590
PspGI CCWGG 1 cut(s) 322
PspLI CGTACG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 63, 321, 672
PspPI GGNCC 1 cut(s) 561
PsuI RGATCY 2 cut(s) 670, 755
RsaI GTAC 2 cut(s) 23, 240
RsaNI GTAC 2 cut(s) 22, 239
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 4 cut(s) 119, 479, 482, 588
Sau3AI GATC 4 cut(s) 550, 670, 755, 770
Sau96I GGNCC 1 cut(s) 561
SchI GAGTC 3 cut(s) 23, 41, 62
ScrFI CCNGG 2 cut(s) 324, 559
SduI GDGCHC 4 cut(s) 44, 90, 381, 453
SinI GGWCC 1 cut(s) 561
Sse9I AATT 5 cut(s) 154, 659, 718, 800, 851
SsiI CCGC 4 cut(s) 335, 353, 481, 505
SspMI CTAG 1 cut(s) 308
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 2 cut(s) 322, 557
StyI CCWWGG 2 cut(s) 275, 465
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 2 cut(s) 222, 345
TaqI TCGA 3 cut(s) 222, 576, 813
TasI AATT 5 cut(s) 154, 659, 718, 800, 851
TatI WGTACW 1 cut(s) 238
TauI GCSGC 1 cut(s) 484
TfiI GAWTC 4 cut(s) 442, 461, 496, 616
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TseFI GTSAC 3 cut(s) 135, 469, 707
TseI GCWGC 3 cut(s) 118, 478, 587
Tsp45I GTSAC 3 cut(s) 135, 469, 707
TspGWI ACGGA 1 cut(s) 39
VneI GTGCAC 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 561
XapI RAATTY 3 cut(s) 659, 800, 851
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.