Prupe.2G270700_v2.0.a1

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0006090
Physical Location & Seq
Forward (+)
27488351 .. 27490358
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G270700.1

Sequence Viewer

Length: 849 bp
ATGGAGGCCCTGATAGCCTCGTACGGAGACTCGTCGTCGGACTCAGACTCCGAGTCGCCAGCTCCACGTCCAGAGCTCAAAAACTCCGAAGAATCGAGCTCTGCTCTGCCCCCACCTCCTCTGTCACTTCTCAACAGTCCCAATTCCTTTGGATTTCTGGACTTCTCGCCAAGCGCTCAGCCCAGCAGAGTGAGGAACTTTCCTCACGTGGAAGGGAACTACGCGGTACATGTCTACATCCCAGTTTATATACCACCAGCACCAAGGAAAGAGATGGCCTTATTTTTGAACAAGCTAGCTTCTTTGGTGCCCGGTCTCCATGCTGTCGACGTTGACGTCCCTCTTGAGATTTTGTGCAAGGATGAGCACAAGCTTGAACAGGTTGCTTTGGGTAGAGAGTTCCATATAAGTCTGGGAAGAACTGTACCAATCCGAGTGCACCAGATTGACTCCCTGGTCACAATGCTTCGGCAGAAGCTTCAGATTCAGAGGCGGTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAACGATGATCACACCCGGACCTTTGTGTCAATAGAAGTTATTGCTGCTGGGTTAGCTGAGATAACAAAGCAGATTCAAGCTGTCAATGAGGTGTATAAGCTTCACAATCTTCCTGAATTTTACAAGGATCCTCGCCCTCATATATCGGTAGCTTGGGCATCGGATGACATCAGCAGTTCCCTGAAGCAAGCGGTTGAGGAAGAAAGAAGATGTACTGTCGGAGGATCATTACAGAAATGTCTTTTTACCAGTAAATTCAATGGCATCGAATGTAGAATTGGCAGTAAAACCCATAAAATATGTAAATTTTCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

283

Amino Acids

31.67

Weight (kDa)

6.22

Isoelectric Point (pI)

62.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 335, 455, 556
AatII GACGTC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 307
AccI GTMKAC 2 cut(s) 234, 327
AccII CGCG 1 cut(s) 224
AciI CCGC 3 cut(s) 224, 493, 722
AclWI GGATC 3 cut(s) 653, 666, 763
AcsI RAATTY 3 cut(s) 647, 785, 836
AcuI CTGAAG 2 cut(s) 464, 734
AcvI CACGTG 1 cut(s) 208
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 4 cut(s) 23, 228, 426, 745
AfeI AGCGCT 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 229
AgsI TTSAA 4 cut(s) 289, 377, 608, 790
AhdI GACNNNNNGTC 2 cut(s) 34, 52
AjiI CACGTC 1 cut(s) 68
AjnI CCWGG 1 cut(s) 453
AjuI GAANNNNNNNTTGG 2 cut(s) 792, 824
Alw21I GWGCWC 4 cut(s) 78, 101, 369, 441
Alw26I GTCTC 2 cut(s) 21, 320
Alw44I GTGCAC 1 cut(s) 437
AlwI GGATC 3 cut(s) 653, 666, 763
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 2 cut(s) 6, 276
ApaLI GTGCAC 1 cut(s) 437
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 3 cut(s) 647, 785, 836
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 2 cut(s) 7, 549
AsuC2I CCSGG 2 cut(s) 312, 547
AsuNHI GCTAGC 1 cut(s) 295
AvaII GGWCC 1 cut(s) 549
BaeGI GKGCMC 2 cut(s) 312, 441
BamHI GGATCC 1 cut(s) 658
BanI GGYRCC 1 cut(s) 307
BanII GRGCYC 2 cut(s) 78, 101
BbrPI CACGTG 1 cut(s) 208
Bbv12I GWGCWC 4 cut(s) 78, 101, 369, 441
BbvI GCAGC 1 cut(s) 562
BccI CCATC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 455
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 2 cut(s) 312, 547
BcoDI GTCTC 2 cut(s) 21, 320
BfaI CTAG 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 1 cut(s) 576
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 577
Bme1390I CCNGG 3 cut(s) 312, 455, 547
Bme18I GGWCC 1 cut(s) 549
BmeRI GACNNNNNGTC 2 cut(s) 34, 52
BmgBI CACGTC 1 cut(s) 68
BmgT120I GGNCC 2 cut(s) 7, 549
BmiI GGNNCC 2 cut(s) 309, 660
BmrFI CCNGG 3 cut(s) 312, 455, 547
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 2 cut(s) 698, 804
BmtI GCTAGC 1 cut(s) 299
BmuI ACTGGG 1 cut(s) 236
BplI GAGNNNNNCTC 2 cut(s) 88, 120
Bpu1102I GCTNAGC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 2 cut(s) 312, 547
BsaAI YACGTR 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 2 cut(s) 263, 453
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bse1I ACTGG 2 cut(s) 242, 780
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 2 cut(s) 263, 453
BseGI GGATG 3 cut(s) 237, 367, 700
BseMII CTCAG 3 cut(s) 57, 191, 579
BseNI ACTGG 2 cut(s) 242, 780
BseRI GAGGAG 1 cut(s) 108
BseSI GKGCMC 2 cut(s) 312, 441
BseXI GCAGC 1 cut(s) 562
BseYI CCCAGC 2 cut(s) 182, 578
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 2 cut(s) 8, 278
BshNI GGYRCC 1 cut(s) 307
BsiHKAI GWGCWC 4 cut(s) 78, 101, 369, 441
BsiSI CCGG 2 cut(s) 312, 547
BsiWI CGTACG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 123, 323
BsmAI GTCTC 2 cut(s) 21, 320
BsmFI GGGAC 2 cut(s) 123, 323
BsnI GGCC 2 cut(s) 8, 278
Bso31I GGTCTC 1 cut(s) 320
Bsp1286I GDGCHC 5 cut(s) 78, 101, 312, 369, 441
Bsp143I GATC 3 cut(s) 538, 658, 755
Bsp1720I GCTNAGC 1 cut(s) 177
BspACI CCGC 3 cut(s) 224, 493, 722
BspANI GGCC 2 cut(s) 8, 278
BspCNI CTCAG 3 cut(s) 56, 190, 580
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 2 cut(s) 309, 660
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 3 cut(s) 653, 666, 763
BspT107I GGYRCC 1 cut(s) 307
BspTNI GGTCTC 1 cut(s) 320
BsrI ACTGG 2 cut(s) 242, 780
BssECI CCNNGG 2 cut(s) 263, 453
BssMI GATC 3 cut(s) 538, 658, 755
BssNI GRCGYC 1 cut(s) 336
BssT1I CCWWGG 1 cut(s) 263
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 3 cut(s) 137, 424, 748
BstACI GRCGYC 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 3 cut(s) 60, 297, 720
BstDEI CTNAG 3 cut(s) 43, 177, 588
BstF5I GGATG 3 cut(s) 237, 367, 700
BstFNI CGCG 1 cut(s) 224
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 3 cut(s) 541, 661, 758
BstMAI GTCTC 2 cut(s) 21, 320
BstMBI GATC 3 cut(s) 538, 658, 755
BstMWI GCNNNNNNNGC 2 cut(s) 14, 584
BstNI CCWGG 1 cut(s) 455
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 310, 453, 545
BstSLI GKGCMC 2 cut(s) 312, 441
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 1 cut(s) 562
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuRI GGCC 2 cut(s) 8, 278
BtrI CACGTC 1 cut(s) 68
BtsCI GGATG 3 cut(s) 237, 367, 700
Cac8I GCNNGC 3 cut(s) 60, 297, 720
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 7, 549
Csp6I GTAC 4 cut(s) 22, 227, 425, 744
CviAII CATG 2 cut(s) 230, 320
CviQI GTAC 4 cut(s) 22, 227, 425, 744
DdeI CTNAG 3 cut(s) 43, 177, 588
DpnI GATC 3 cut(s) 540, 660, 757
DpnII GATC 3 cut(s) 538, 658, 755
DrdI GACNNNNNNGTC 3 cut(s) 335, 455, 556
DriI GACNNNNNGTC 2 cut(s) 34, 52
DseDI GACNNNNNNGTC 3 cut(s) 335, 455, 556
Eam1105I GACNNNNNGTC 2 cut(s) 34, 52
Ecl136II GAGCTC 2 cut(s) 76, 99
Eco130I CCWWGG 1 cut(s) 263
Eco24I GRGCYC 2 cut(s) 78, 101
Eco31I GGTCTC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 549
Eco47III AGCGCT 1 cut(s) 175
Eco53kI GAGCTC 2 cut(s) 76, 99
Eco57I CTGAAG 2 cut(s) 464, 734
Eco72I CACGTG 1 cut(s) 208
EcoICRI GAGCTC 2 cut(s) 76, 99
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 263
EcoT38I GRGCYC 2 cut(s) 78, 101
ErhI CCWWGG 1 cut(s) 263
FaeI CATG 2 cut(s) 233, 323
FaqI GGGAC 2 cut(s) 123, 323
FatI CATG 2 cut(s) 229, 319
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 2 cut(s) 234, 327
Fnu4HI GCNGC 1 cut(s) 576
FokI GGATG 3 cut(s) 224, 374, 707
FriOI GRGCYC 2 cut(s) 78, 101
Fsp4HI GCNGC 1 cut(s) 576
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 175
GluI GCNGC 1 cut(s) 576
GsaI CCCAGC 2 cut(s) 186, 582
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 2 cut(s) 8, 278
HapII CCGG 2 cut(s) 312, 547
HhaI GCGC 1 cut(s) 176
Hin1I GRCGYC 1 cut(s) 336
Hin1II CATG 2 cut(s) 233, 323
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 3 cut(s) 328, 334, 532
HindII GTYRAC 3 cut(s) 328, 334, 532
HindIII AAGCTT 3 cut(s) 371, 476, 629
HinfI GANTC 8 cut(s) 29, 41, 47, 53, 92, 449, 484, 604
HpaI GTTAAC 1 cut(s) 532
HpaII CCGG 2 cut(s) 312, 547
Hpy166II GTNNAC 5 cut(s) 235, 328, 334, 439, 532
Hpy188III TCNNGA 4 cut(s) 71, 158, 344, 644
Hpy8I GTNNAC 5 cut(s) 235, 328, 334, 439, 532
Hpy99I CGWCG 3 cut(s) 37, 40, 332
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 3 cut(s) 137, 424, 748
HpyCH4IV ACGT 4 cut(s) 67, 207, 330, 336
HpyCH4V TGCA 2 cut(s) 357, 439
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 584
HpyF3I CTNAG 3 cut(s) 43, 177, 588
HpySE526I ACGT 4 cut(s) 67, 207, 330, 336
Hsp92I GRCGYC 1 cut(s) 336
Hsp92II CATG 2 cut(s) 233, 323
HspAI GCGC 1 cut(s) 174
Ksp22I TGATCA 1 cut(s) 538
KspAI GTTAAC 1 cut(s) 532
Kzo9I GATC 3 cut(s) 538, 658, 755
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 2 cut(s) 698, 804
MaeI CTAG 1 cut(s) 296
MaeII ACGT 4 cut(s) 67, 207, 330, 336
MaeIII GTNAC 2 cut(s) 123, 457
MalI GATC 3 cut(s) 540, 660, 757
MboI GATC 3 cut(s) 538, 658, 755
MboII GAAGA 5 cut(s) 101, 429, 632, 743, 750
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 5 cut(s) 78, 101, 312, 369, 441
MluCI AATT 5 cut(s) 142, 647, 785, 807, 836
MlyI GAGTC 5 cut(s) 23, 35, 41, 62, 443
MmeI TCCRAC 2 cut(s) 18, 730
MseI TTAA 1 cut(s) 531
MspI CCGG 2 cut(s) 312, 547
MspR9I CCNGG 3 cut(s) 312, 455, 547
MvaI CCWGG 1 cut(s) 455
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 14, 584
NciI CCSGG 2 cut(s) 312, 547
NdeII GATC 3 cut(s) 538, 658, 755
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 2 cut(s) 233, 323
NlaIV GGNNCC 2 cut(s) 309, 660
NmuCI GTSAC 2 cut(s) 123, 457
NspI RCATGY 1 cut(s) 233
PciI ACATGT 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PfeI GAWTC 3 cut(s) 92, 484, 604
Pfl23II CGTACG 1 cut(s) 21
PkrI GCNGC 1 cut(s) 577
PleI GAGTC 5 cut(s) 23, 35, 41, 61, 443
PmaCI CACGTG 1 cut(s) 208
PmlI CACGTG 1 cut(s) 208
PpsI GAGTC 5 cut(s) 23, 35, 41, 61, 443
Ppu21I YACGTR 1 cut(s) 208
PscI ACATGT 1 cut(s) 229
Psp124BI GAGCTC 2 cut(s) 78, 101
Psp6I CCWGG 1 cut(s) 453
PspCI CACGTG 1 cut(s) 208
PspFI CCCAGC 2 cut(s) 182, 578
PspGI CCWGG 1 cut(s) 453
PspLI CGTACG 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 309, 660
PspPI GGNCC 2 cut(s) 7, 549
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 4 cut(s) 23, 228, 426, 745
RsaNI GTAC 4 cut(s) 22, 227, 425, 744
SacI GAGCTC 2 cut(s) 78, 101
SalI GTCGAC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 1 cut(s) 576
Sau3AI GATC 3 cut(s) 538, 658, 755
Sau96I GGNCC 2 cut(s) 7, 549
SchI GAGTC 5 cut(s) 23, 35, 41, 62, 443
ScrFI CCNGG 3 cut(s) 312, 455, 547
SduI GDGCHC 5 cut(s) 78, 101, 312, 369, 441
SfaNI GCATC 2 cut(s) 698, 804
SinI GGWCC 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 5 cut(s) 142, 647, 785, 807, 836
SsiI CCGC 3 cut(s) 224, 493, 722
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 2 cut(s) 78, 101
StyD4I CCNGG 3 cut(s) 310, 453, 545
StyI CCWWGG 1 cut(s) 263
TaaI ACNGT 3 cut(s) 137, 424, 748
TaiI ACGT 4 cut(s) 70, 210, 333, 339
TaqI TCGA 3 cut(s) 95, 327, 798
TasI AATT 5 cut(s) 142, 647, 785, 807, 836
TatI WGTACW 1 cut(s) 743
TfiI GAWTC 3 cut(s) 92, 484, 604
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TseFI GTSAC 2 cut(s) 123, 457
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 2 cut(s) 123, 457
TspGWI ACGGA 1 cut(s) 39
VneI GTGCAC 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 549
XapI RAATTY 3 cut(s) 647, 785, 836
XceI RCATGY 1 cut(s) 233
XmiI GTMKAC 2 cut(s) 234, 327
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.