Rw1G034890

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006090
Physical Location & Seq
Reverse (-)
62747941 .. 62749673
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G034890.1

Sequence Viewer

Length: 786 bp
ATGGAGGCACTGATAGCATCTTACGGAGACACGTCGTCGGACTCAGACTCCGAGTCGTCGCCGGCGCCACCGTCGGAGCAGAAAACGTCTCAAGAATCAACCCTCGCTCAGCCCAGCAGAGTTAGGAACTTCCCTCATATAGAAGGAAACTACGCTCTACATGTCTATATCCCAGTGTATATACCGGCGGCGCCGAGGAAGGAGATGGCATTGTTTATGAGGAGACTGTCATCTGTTGTGCCTGGTCTCCATGCTGTGGACGTTGATGTGCCGCTTGACGTTTTGTGCAAAGATGAACAAAAGCTTGAACAGGTTGGGTTAGGGAGAGAGTTTCATATAAGCCTGGGGAGGACTGTGCCGATCAGGGTGCACCAGATTGACTCATTGGTGGCAATGCTGAAGCAGAAGCTTCAGACTCAGAGGCGTTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAACGATGATCATACACGCACCTTTGTATCAGTGGAAGTTATTACTGCTGGATTAGCTGAGATAACAAAGCAGATTCAAGCTGTCAATGAGGTGTATAAGCTTCACAATCTCCCTGAGTTTTACAAGGATCCACGTCCCCATATATCAGTCGCTTGGGCATTGGGTGACACCAGGGAATCCCTAAAGAAAGTGGTTGAAGATGAAAGAAGAAAATCTACAGTTGGGGGGTCATTGCAGAAATGTATTTTTACCAGTAGATTCAGTGGCATAGACTGTAAGATAGGCAACAAAACATATAAAATATGTAAATTTTCTGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

261

Amino Acids

29.55

Weight (kDa)

7.09

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 26 - 256 5e-57 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 64, 190
AccB7I CCANNNNNTGG 1 cut(s) 256
AciI CCGC 2 cut(s) 188, 272
AclWI GGATC 2 cut(s) 584, 597
AcsI RAATTY 1 cut(s) 770
AcuI CTGAAG 2 cut(s) 395, 419
AcyI GRCGYC 2 cut(s) 65, 191
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 2 cut(s) 30, 160
AgsI TTSAA 3 cut(s) 308, 539, 659
AhdI GACNNNNNGTC 2 cut(s) 34, 52
AjiI CACGTC 2 cut(s) 33, 596
AjnI CCWGG 3 cut(s) 241, 342, 632
AluBI AGCT 5 cut(s) 304, 409, 518, 542, 562
AluI AGCT 5 cut(s) 304, 409, 518, 542, 562
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 4 cut(s) 21, 93, 217, 251
Alw44I GTGCAC 1 cut(s) 368
AlwI GGATC 2 cut(s) 584, 597
ApaLI GTGCAC 1 cut(s) 368
ApoI RAATTY 1 cut(s) 770
AspLEI GCGC 2 cut(s) 67, 193
AsuHPI GGTGA 1 cut(s) 638
BaeGI GKGCMC 1 cut(s) 372
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BamHI GGATCC 1 cut(s) 589
BanI GGYRCC 2 cut(s) 64, 190
BarI GAAGNNNNNNTAC 2 cut(s) 661, 693
Bbv12I GWGCWC 1 cut(s) 372
BccI CCATC 1 cut(s) 199
BcgI CGANNNNNNTGC 2 cut(s) 349, 383
BciT130I CCWGG 3 cut(s) 243, 344, 634
BclI TGATCA 1 cut(s) 469
BcoDI GTCTC 4 cut(s) 21, 93, 217, 251
BfmI CTRYAG 1 cut(s) 678
BfoI RGCGCY 2 cut(s) 68, 194
BisI GCNGC 2 cut(s) 189, 272
BlpI GCTNAGC 1 cut(s) 108
BlsI GCNGC 2 cut(s) 190, 273
Bme1390I CCNGG 3 cut(s) 243, 344, 634
BmeRI GACNNNNNGTC 2 cut(s) 34, 52
BmgBI CACGTC 2 cut(s) 33, 596
BmiI GGNNCC 3 cut(s) 66, 192, 591
BmrFI CCNGG 3 cut(s) 243, 344, 634
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 167
Bpu1102I GCTNAGC 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 65, 191
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 194, 343, 633
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse118I RCCGGY 2 cut(s) 61, 184
Bse1I ACTGG 2 cut(s) 173, 714
Bse3DI GCAATG 2 cut(s) 399, 692
BseBI CCWGG 3 cut(s) 243, 344, 634
BseDI CCNNGG 3 cut(s) 194, 343, 633
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMI GCAATG 2 cut(s) 399, 692
BseMII CTCAG 5 cut(s) 57, 122, 431, 510, 567
BseNI ACTGG 2 cut(s) 173, 714
BseRI GAGGAG 1 cut(s) 235
BseSI GKGCMC 1 cut(s) 372
BseYI CCCAGC 1 cut(s) 113
BshNI GGYRCC 2 cut(s) 64, 190
BsiHKAI GWGCWC 1 cut(s) 372
BsiSI CCGG 2 cut(s) 62, 185
BslFI GGGAC 1 cut(s) 582
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 4 cut(s) 21, 93, 217, 251
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 582
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 372
Bsp143I GATC 3 cut(s) 360, 469, 589
Bsp1720I GCTNAGC 1 cut(s) 108
BspACI CCGC 2 cut(s) 188, 272
BspCNI CTCAG 5 cut(s) 56, 121, 430, 511, 568
BspLI GGNNCC 3 cut(s) 66, 192, 591
BspPI GGATC 2 cut(s) 584, 597
BspT107I GGYRCC 2 cut(s) 64, 190
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 2 cut(s) 399, 692
BsrFI RCCGGY 2 cut(s) 61, 184
BsrI ACTGG 2 cut(s) 173, 714
BssAI RCCGGY 2 cut(s) 61, 184
BssECI CCNNGG 3 cut(s) 194, 343, 633
BssMI GATC 3 cut(s) 360, 469, 589
BssNI GRCGYC 2 cut(s) 65, 191
Bst2UI CCWGG 3 cut(s) 243, 344, 634
Bst4CI ACNGT 5 cut(s) 72, 228, 355, 682, 737
BstACI GRCGYC 2 cut(s) 65, 191
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 5 cut(s) 43, 108, 417, 519, 576
BstH2I RGCGCY 2 cut(s) 68, 194
BstHHI GCGC 2 cut(s) 67, 193
BstKTI GATC 3 cut(s) 363, 472, 592
BstMAI GTCTC 4 cut(s) 21, 93, 217, 251
BstMBI GATC 3 cut(s) 360, 469, 589
BstMWI GCNNNNNNNGC 2 cut(s) 14, 515
BstNI CCWGG 3 cut(s) 243, 344, 634
BstNSI RCATGY 1 cut(s) 164
BstSCI CCNGG 3 cut(s) 241, 342, 632
BstSFI CTRYAG 1 cut(s) 678
BstSLI GKGCMC 1 cut(s) 372
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BtrI CACGTC 2 cut(s) 33, 596
BtsIMutI CAGTG 4 cut(s) 8, 180, 498, 730
Cac8I GCNNGC 1 cut(s) 63
CfoI GCGC 2 cut(s) 67, 193
Cfr10I RCCGGY 2 cut(s) 61, 184
CviAII CATG 2 cut(s) 161, 251
CviJI RGCY 7 cut(s) 112, 304, 342, 409, 518, 542, 562
CviKI_1 RGCY 7 cut(s) 112, 304, 342, 409, 518, 542, 562
DdeI CTNAG 5 cut(s) 43, 108, 417, 519, 576
DinI GGCGCC 2 cut(s) 66, 192
DpnI GATC 3 cut(s) 362, 471, 591
DpnII GATC 3 cut(s) 360, 469, 589
DriI GACNNNNNGTC 2 cut(s) 34, 52
Eam1105I GACNNNNNGTC 2 cut(s) 34, 52
Eco31I GGTCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 395, 419
EcoRII CCWGG 3 cut(s) 241, 342, 632
EgeI GGCGCC 2 cut(s) 66, 192
EheI GGCGCC 2 cut(s) 66, 192
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 2 cut(s) 164, 254
FaqI GGGAC 1 cut(s) 582
FatI CATG 2 cut(s) 160, 250
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 189, 272
Fsp4HI GCNGC 2 cut(s) 189, 272
GlaI GCGC 2 cut(s) 66, 192
GluI GCNGC 2 cut(s) 189, 272
GsaI CCCAGC 1 cut(s) 117
HaeII RGCGCY 2 cut(s) 68, 194
HapII CCGG 2 cut(s) 62, 185
HhaI GCGC 2 cut(s) 67, 193
Hin1I GRCGYC 2 cut(s) 65, 191
Hin1II CATG 2 cut(s) 164, 254
Hin6I GCGC 2 cut(s) 65, 191
HinP1I GCGC 2 cut(s) 65, 191
HincII GTYRAC 1 cut(s) 463
HindII GTYRAC 1 cut(s) 463
HindIII AAGCTT 3 cut(s) 302, 407, 560
HinfI GANTC 9 cut(s) 41, 47, 53, 95, 380, 415, 535, 638, 720
HpaI GTTAAC 1 cut(s) 463
HpaII CCGG 2 cut(s) 62, 185
HphI GGTGA 1 cut(s) 638
Hpy166II GTNNAC 3 cut(s) 259, 370, 463
Hpy188I TCNGA 7 cut(s) 40, 46, 52, 76, 414, 420, 778
Hpy188III TCNNGA 1 cut(s) 92
Hpy8I GTNNAC 3 cut(s) 259, 370, 463
Hpy99I CGWCG 4 cut(s) 37, 40, 61, 76
HpyAV CCTTC 2 cut(s) 137, 193
HpyCH4III ACNGT 5 cut(s) 72, 228, 355, 682, 737
HpyCH4IV ACGT 5 cut(s) 32, 86, 261, 279, 595
HpyCH4V TGCA 3 cut(s) 288, 370, 697
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 515
HpyF3I CTNAG 5 cut(s) 43, 108, 417, 519, 576
HpySE526I ACGT 5 cut(s) 32, 86, 261, 279, 595
Hsp92I GRCGYC 2 cut(s) 65, 191
Hsp92II CATG 2 cut(s) 164, 254
HspAI GCGC 2 cut(s) 65, 191
KasI GGCGCC 2 cut(s) 64, 190
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 463
Kzo9I GATC 3 cut(s) 360, 469, 589
LmnI GCTCC 1 cut(s) 76
LweI GCATC 1 cut(s) 26
MaeII ACGT 5 cut(s) 32, 86, 261, 279, 595
MaeIII GTNAC 1 cut(s) 626
MalI GATC 3 cut(s) 362, 471, 591
MboI GATC 3 cut(s) 360, 469, 589
MboII GAAGA 2 cut(s) 671, 681
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 372
MluCI AATT 1 cut(s) 770
Mly113I GGCGCC 2 cut(s) 65, 191
MlyI GAGTC 5 cut(s) 35, 41, 62, 374, 409
MmeI TCCRAC 2 cut(s) 18, 54
MnlI CCTC 8 cut(s) 113, 144, 189, 213, 342, 414, 445, 544
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MseI TTAA 1 cut(s) 462
MspI CCGG 2 cut(s) 62, 185
MspR9I CCNGG 3 cut(s) 243, 344, 634
MvaI CCWGG 3 cut(s) 243, 344, 634
MwoI GCNNNNNNNGC 2 cut(s) 14, 515
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 2 cut(s) 65, 191
NdeII GATC 3 cut(s) 360, 469, 589
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 2 cut(s) 164, 254
NlaIV GGNNCC 3 cut(s) 66, 192, 591
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 1 cut(s) 626
NspI RCATGY 1 cut(s) 164
PciI ACATGT 1 cut(s) 160
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 95, 535, 638, 720
PflMI CCANNNNNTGG 1 cut(s) 256
PkrI GCNGC 2 cut(s) 190, 273
PleI GAGTC 5 cut(s) 35, 41, 61, 374, 409
PluTI GGCGCC 2 cut(s) 68, 194
PpsI GAGTC 5 cut(s) 35, 41, 61, 374, 409
PscI ACATGT 1 cut(s) 160
Psp6I CCWGG 3 cut(s) 241, 342, 632
PspFI CCCAGC 1 cut(s) 113
PspGI CCWGG 3 cut(s) 241, 342, 632
PspN4I GGNNCC 3 cut(s) 66, 192, 591
PsuI RGATCY 1 cut(s) 589
SaqAI TTAA 1 cut(s) 462
SatI GCNGC 2 cut(s) 189, 272
Sau3AI GATC 3 cut(s) 360, 469, 589
SchI GAGTC 5 cut(s) 35, 41, 62, 374, 409
ScrFI CCNGG 3 cut(s) 243, 344, 634
SduI GDGCHC 1 cut(s) 372
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 1 cut(s) 678
SfoI GGCGCC 2 cut(s) 66, 192
SgrAI CRCCGGYG 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 1 cut(s) 770
SsiI CCGC 2 cut(s) 188, 272
SspDI GGCGCC 2 cut(s) 64, 190
StyD4I CCNGG 3 cut(s) 241, 342, 632
TaaI ACNGT 5 cut(s) 72, 228, 355, 682, 737
TaiI ACGT 5 cut(s) 35, 89, 264, 282, 598
TasI AATT 1 cut(s) 770
TauI GCSGC 2 cut(s) 191, 274
TfiI GAWTC 4 cut(s) 95, 535, 638, 720
Tru1I TTAA 1 cut(s) 462
Tru9I TTAA 1 cut(s) 462
TscAI CASTG 4 cut(s) 15, 180, 498, 730
TseFI GTSAC 1 cut(s) 626
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 3 cut(s) 309, 323, 678
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 4 cut(s) 15, 180, 498, 730
Van91I CCANNNNNTGG 1 cut(s) 256
VneI GTGCAC 1 cut(s) 368
XapI RAATTY 1 cut(s) 770
XceI RCATGY 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.