Rh1AG386100

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0006090
Physical Location & Seq
Forward (+)
61668383 .. 61670539
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG386100.1

Sequence Viewer

Length: 858 bp
ATGGAGGCACTGATAGCATCTTACGGAGACACGTCGTCGGACTCAGACTCCGAGTCGTCGCCGGCGCCACCGTCGGAGCAGAAAACGTCTCAAGAATCAACTCCTGTTCTTCCTCCGCCTCCGCTCTCACTTCTCGACACCCCCAATTCATTAGGATTTCTGGACTACTTGCCGAGCGCTCAGCCCAGCAGAGTTAGGAACTTCCCTCATATAGAAGGAAACTACGCTCTACATGTCTATATCCCAGTGTATATACCGGCGGCGCCGAGGAAGGAGATGGCATTGTTTATGAGGAGACTGTCATCTGTTGTGCCTGGTCTCCATGCTGTGGACGTTGATGTGCCGCTTGACGTTTTGTGCAAAGATGAACAAAAGCTTGAACAGGTTGGGTTAGGGAGAGAGTTTCATATAAGCCTGGGGAGGACTGTGCCGATCAGGGTGCACCAGATTGACTCATTGGTGACAATGCTGAAGCAGAAGCTTCAGACTCAGAGGCGTTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAACGATGACCATACACGCACCTTTGTATCAGTGGAAGTTATTACTGCTGGATTAGCTGAGATAACAAAGCAGATTCAAGCTGTCAATGAGGTGTATAAGCTTCACAATCTCCCTGAGTTTTACAAGGATCCACGTCCCCATATATCAGTCGCTTGGGCATTGGGTGACACCAGGGAATCCCTAAAGAAAGTGGTTGAAGATGAAAGAAGAAAATCTACAGTTGGGGGGTCATTGCAGAAATGTATTTTTACCAGTAGATTCAGTGGCATAGACTGTAAGATAGGCAACAAAACATATAAAATATGTAAATTTTCTGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

285

Amino Acids

32.11

Weight (kDa)

6.46

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 38 - 280 1.5e-57 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 64, 262
AccB7I CCANNNNNTGG 1 cut(s) 328
AccBSI CCGCTC 1 cut(s) 124
AciI CCGC 4 cut(s) 116, 122, 260, 344
AclWI GGATC 2 cut(s) 656, 669
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 2 cut(s) 467, 491
AcyI GRCGYC 2 cut(s) 65, 263
AfeI AGCGCT 1 cut(s) 178
AfiI CCNNNNNNNGG 1 cut(s) 328
AflIII ACRYGT 2 cut(s) 30, 232
AgsI TTSAA 3 cut(s) 380, 611, 731
AhdI GACNNNNNGTC 2 cut(s) 34, 52
AjiI CACGTC 2 cut(s) 33, 668
AjnI CCWGG 3 cut(s) 313, 414, 704
AluBI AGCT 5 cut(s) 376, 481, 590, 614, 634
AluI AGCT 5 cut(s) 376, 481, 590, 614, 634
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 4 cut(s) 21, 93, 289, 323
Alw44I GTGCAC 1 cut(s) 440
AlwI GGATC 2 cut(s) 656, 669
Aor51HI AGCGCT 1 cut(s) 178
ApaLI GTGCAC 1 cut(s) 440
ApoI RAATTY 1 cut(s) 842
AspLEI GCGC 3 cut(s) 67, 179, 265
AsuHPI GGTGA 2 cut(s) 472, 710
BaeGI GKGCMC 1 cut(s) 444
BaeI ACNNNNGTAYC 2 cut(s) 543, 576
BamHI GGATCC 1 cut(s) 661
BanI GGYRCC 2 cut(s) 64, 262
BarI GAAGNNNNNNTAC 2 cut(s) 733, 765
Bbv12I GWGCWC 1 cut(s) 444
BccI CCATC 1 cut(s) 271
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BciT130I CCWGG 3 cut(s) 315, 416, 706
BcoDI GTCTC 4 cut(s) 21, 93, 289, 323
BfmI CTRYAG 1 cut(s) 750
BfoI RGCGCY 3 cut(s) 68, 180, 266
BisI GCNGC 2 cut(s) 261, 344
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 2 cut(s) 262, 345
Bme1390I CCNGG 3 cut(s) 315, 416, 706
BmeRI GACNNNNNGTC 2 cut(s) 34, 52
BmgBI CACGTC 2 cut(s) 33, 668
BmiI GGNNCC 3 cut(s) 66, 264, 663
BmrFI CCNGG 3 cut(s) 315, 416, 706
BmrI ACTGGG 1 cut(s) 239
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 239
Bpu1102I GCTNAGC 1 cut(s) 180
BpuEI CTTGAG 1 cut(s) 75
BsaHI GRCGYC 2 cut(s) 65, 263
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 266, 415, 705
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse118I RCCGGY 2 cut(s) 61, 256
Bse1I ACTGG 2 cut(s) 245, 786
Bse3DI GCAATG 1 cut(s) 764
BseBI CCWGG 3 cut(s) 315, 416, 706
BseDI CCNNGG 3 cut(s) 266, 415, 705
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 764
BseMII CTCAG 5 cut(s) 57, 194, 503, 582, 639
BseNI ACTGG 2 cut(s) 245, 786
BseRI GAGGAG 1 cut(s) 307
BseSI GKGCMC 1 cut(s) 444
BseYI CCCAGC 1 cut(s) 185
BshNI GGYRCC 2 cut(s) 64, 262
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 2 cut(s) 62, 257
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 4 cut(s) 21, 93, 289, 323
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 654
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 2 cut(s) 432, 661
Bsp1720I GCTNAGC 1 cut(s) 180
BspACI CCGC 4 cut(s) 116, 122, 260, 344
BspCNI CTCAG 5 cut(s) 56, 193, 502, 583, 640
BspLI GGNNCC 3 cut(s) 66, 264, 663
BspPI GGATC 2 cut(s) 656, 669
BspT107I GGYRCC 2 cut(s) 64, 262
BspTNI GGTCTC 1 cut(s) 323
BsrBI CCGCTC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 764
BsrFI RCCGGY 2 cut(s) 61, 256
BsrI ACTGG 2 cut(s) 245, 786
BssAI RCCGGY 2 cut(s) 61, 256
BssECI CCNNGG 3 cut(s) 266, 415, 705
BssMI GATC 2 cut(s) 432, 661
BssNI GRCGYC 2 cut(s) 65, 263
Bst2UI CCWGG 3 cut(s) 315, 416, 706
Bst4CI ACNGT 5 cut(s) 72, 300, 427, 754, 809
BstACI GRCGYC 2 cut(s) 65, 263
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 5 cut(s) 43, 180, 489, 591, 648
BstH2I RGCGCY 3 cut(s) 68, 180, 266
BstHHI GCGC 3 cut(s) 67, 179, 265
BstKTI GATC 2 cut(s) 435, 664
BstMAI GTCTC 4 cut(s) 21, 93, 289, 323
BstMBI GATC 2 cut(s) 432, 661
BstMWI GCNNNNNNNGC 2 cut(s) 14, 587
BstNI CCWGG 3 cut(s) 315, 416, 706
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 3 cut(s) 313, 414, 704
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 1 cut(s) 444
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BtrI CACGTC 2 cut(s) 33, 668
BtsIMutI CAGTG 4 cut(s) 8, 252, 570, 802
Cac8I GCNNGC 1 cut(s) 63
CfoI GCGC 3 cut(s) 67, 179, 265
Cfr10I RCCGGY 2 cut(s) 61, 256
CviAII CATG 2 cut(s) 233, 323
CviJI RGCY 7 cut(s) 184, 376, 414, 481, 590, 614, 634
CviKI_1 RGCY 7 cut(s) 184, 376, 414, 481, 590, 614, 634
DdeI CTNAG 5 cut(s) 43, 180, 489, 591, 648
DinI GGCGCC 2 cut(s) 66, 264
DpnI GATC 2 cut(s) 434, 663
DpnII GATC 2 cut(s) 432, 661
DriI GACNNNNNGTC 2 cut(s) 34, 52
Eam1105I GACNNNNNGTC 2 cut(s) 34, 52
EciI GGCGGA 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 323
Eco47III AGCGCT 1 cut(s) 178
Eco57I CTGAAG 2 cut(s) 467, 491
EcoRII CCWGG 3 cut(s) 313, 414, 704
EgeI GGCGCC 2 cut(s) 66, 264
EheI GGCGCC 2 cut(s) 66, 264
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 2 cut(s) 236, 326
FaqI GGGAC 1 cut(s) 654
FatI CATG 2 cut(s) 232, 322
Fnu4HI GCNGC 2 cut(s) 261, 344
Fsp4HI GCNGC 2 cut(s) 261, 344
GlaI GCGC 3 cut(s) 66, 178, 264
GluI GCNGC 2 cut(s) 261, 344
GsaI CCCAGC 1 cut(s) 189
HaeII RGCGCY 3 cut(s) 68, 180, 266
HapII CCGG 2 cut(s) 62, 257
HhaI GCGC 3 cut(s) 67, 179, 265
Hin1I GRCGYC 2 cut(s) 65, 263
Hin1II CATG 2 cut(s) 236, 326
Hin6I GCGC 3 cut(s) 65, 177, 263
HinP1I GCGC 3 cut(s) 65, 177, 263
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HindIII AAGCTT 3 cut(s) 374, 479, 632
HinfI GANTC 9 cut(s) 41, 47, 53, 95, 452, 487, 607, 710, 792
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 2 cut(s) 62, 257
HphI GGTGA 2 cut(s) 472, 710
Hpy166II GTNNAC 3 cut(s) 331, 442, 535
Hpy188I TCNGA 7 cut(s) 40, 46, 52, 76, 486, 492, 850
Hpy188III TCNNGA 3 cut(s) 92, 134, 161
Hpy8I GTNNAC 3 cut(s) 331, 442, 535
Hpy99I CGWCG 4 cut(s) 37, 40, 61, 76
HpyAV CCTTC 2 cut(s) 209, 265
HpyCH4III ACNGT 5 cut(s) 72, 300, 427, 754, 809
HpyCH4IV ACGT 5 cut(s) 32, 86, 333, 351, 667
HpyCH4V TGCA 3 cut(s) 360, 442, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 587
HpyF3I CTNAG 5 cut(s) 43, 180, 489, 591, 648
HpySE526I ACGT 5 cut(s) 32, 86, 333, 351, 667
Hsp92I GRCGYC 2 cut(s) 65, 263
Hsp92II CATG 2 cut(s) 236, 326
HspAI GCGC 3 cut(s) 65, 177, 263
KasI GGCGCC 2 cut(s) 64, 262
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 2 cut(s) 432, 661
LmnI GCTCC 1 cut(s) 76
LweI GCATC 1 cut(s) 26
MaeII ACGT 5 cut(s) 32, 86, 333, 351, 667
MaeIII GTNAC 2 cut(s) 460, 698
MalI GATC 2 cut(s) 434, 663
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 432, 661
MboII GAAGA 3 cut(s) 101, 743, 753
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 2 cut(s) 145, 842
Mly113I GGCGCC 2 cut(s) 65, 263
MlyI GAGTC 5 cut(s) 35, 41, 62, 446, 481
MmeI TCCRAC 2 cut(s) 18, 54
MnlI CCTC 9 cut(s) 123, 129, 216, 261, 285, 414, 486, 517, 616
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MseI TTAA 1 cut(s) 534
MspI CCGG 2 cut(s) 62, 257
MspR9I CCNGG 3 cut(s) 315, 416, 706
MvaI CCWGG 3 cut(s) 315, 416, 706
MwoI GCNNNNNNNGC 2 cut(s) 14, 587
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 2 cut(s) 65, 263
NdeII GATC 2 cut(s) 432, 661
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 2 cut(s) 236, 326
NlaIV GGNNCC 3 cut(s) 66, 264, 663
NmeAIII GCCGAG 2 cut(s) 198, 291
NmuCI GTSAC 2 cut(s) 460, 698
NspI RCATGY 1 cut(s) 236
PciI ACATGT 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 95, 607, 710, 792
PflMI CCANNNNNTGG 1 cut(s) 328
PkrI GCNGC 2 cut(s) 262, 345
PleI GAGTC 5 cut(s) 35, 41, 61, 446, 481
PluTI GGCGCC 2 cut(s) 68, 266
PpsI GAGTC 5 cut(s) 35, 41, 61, 446, 481
PscI ACATGT 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 313, 414, 704
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 3 cut(s) 313, 414, 704
PspN4I GGNNCC 3 cut(s) 66, 264, 663
PsuI RGATCY 1 cut(s) 661
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 2 cut(s) 261, 344
Sau3AI GATC 2 cut(s) 432, 661
SchI GAGTC 5 cut(s) 35, 41, 62, 446, 481
ScrFI CCNGG 3 cut(s) 315, 416, 706
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 1 cut(s) 26
SfcI CTRYAG 1 cut(s) 750
SfoI GGCGCC 2 cut(s) 66, 264
SgrAI CRCCGGYG 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 2 cut(s) 145, 842
SsiI CCGC 4 cut(s) 116, 122, 260, 344
SspDI GGCGCC 2 cut(s) 64, 262
StyD4I CCNGG 3 cut(s) 313, 414, 704
TaaI ACNGT 5 cut(s) 72, 300, 427, 754, 809
TaiI ACGT 5 cut(s) 35, 89, 336, 354, 670
TaqI TCGA 1 cut(s) 135
TasI AATT 2 cut(s) 145, 842
TauI GCSGC 2 cut(s) 263, 346
TfiI GAWTC 4 cut(s) 95, 607, 710, 792
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 4 cut(s) 15, 252, 570, 802
TseFI GTSAC 2 cut(s) 460, 698
Tsp45I GTSAC 2 cut(s) 460, 698
TspDTI ATGAA 4 cut(s) 138, 381, 395, 750
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 4 cut(s) 15, 252, 570, 802
Van91I CCANNNNNTGG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 440
XapI RAATTY 1 cut(s) 842
XceI RCATGY 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.