Rh1BG350200

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0006090
Physical Location & Seq
Forward (+)
48321460 .. 48323325
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG350200.1

Sequence Viewer

Length: 861 bp
ATGGAGGCACTGATAGCTTCTTACGGAGACACGTCGTCGGACTCAGACTCCGAGTCGTCGCCGGCGCCACCGCCTGAGCAGAAAACGTCTCAAGAATCAACAGCTGTTCTTCTTCCTCCGCCTCCTCTCTCACTTCTCGACGCCCCCAATTCATTAGGATTTCTGGACTACTTGCCGAGCGCACAGCCGAGCAGAGTTAGGAACTTCCCTCATATAGAAGGAAACTACGCTCTACATGTCTATATCCCAGTTTATATACCGGCGGCGCCAAGGAAGGAGATGGCATTGTTTATGAGGAGACTGTCATCTGTTGTGCCTGGTCTCCATGCTGTGGACGTTGATGTGCCGCTTGACGTTTTGTGCAAAGATGAACAAAAGCTTGAACAGGTTGGGTTAGGGAGGGAGTTTCATATAAGTCTGGGGAGGACTGTGCCGATCCGGGTGCACCAGATTGACTCATTGGTGGCAATGCTGAAGCAGAAGCTTCAGACTCAGAGGCGTTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAACGATGATCATACACGCACCTTTGTATCAGTGGAAGTTATTACTGCTGGATTAGCTGAGATAACAAAGCAGATTCAAGCTGTCAATGAGGTGTATAAGCTTCACAATCTCCCTGAGTTTTACAAGGATCCACGTCCCCATATATCAGTCGCTTGGGCATTGGGTGACACCAGGGAATCCCTAAAGAAAGTGGTTGAAGATGAAAGAAGAAAATCTACAGTTGGGGGGTCATTGCAGAAATGTATTTTTACCAGTAGATTCAGTGGCATAGACTGTAAGATTGGCAACAAAACATATAAAATATGTAAATTTTCTGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

286

Amino Acids

32.15

Weight (kDa)

6.46

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 39 - 281 2.2e-57 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 64, 265
AccB7I CCANNNNNTGG 1 cut(s) 331
AciI CCGC 4 cut(s) 71, 119, 263, 347
AclWI GGATC 3 cut(s) 430, 659, 672
AcsI RAATTY 1 cut(s) 845
AcuI CTGAAG 2 cut(s) 470, 494
AcyI GRCGYC 3 cut(s) 65, 141, 266
AfiI CCNNNNNNNGG 1 cut(s) 331
AflIII ACRYGT 2 cut(s) 30, 235
AgsI TTSAA 3 cut(s) 383, 614, 734
AhdI GACNNNNNGTC 2 cut(s) 34, 52
AjiI CACGTC 2 cut(s) 33, 671
AjnI CCWGG 2 cut(s) 316, 707
AluBI AGCT 7 cut(s) 17, 104, 379, 484, 593, 617, 637
AluI AGCT 7 cut(s) 17, 104, 379, 484, 593, 617, 637
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 4 cut(s) 21, 93, 292, 326
Alw44I GTGCAC 1 cut(s) 443
AlwI GGATC 3 cut(s) 430, 659, 672
ApaLI GTGCAC 1 cut(s) 443
ApoI RAATTY 1 cut(s) 845
AspLEI GCGC 3 cut(s) 67, 182, 268
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 1 cut(s) 713
BaeGI GKGCMC 1 cut(s) 447
BaeI ACNNNNGTAYC 2 cut(s) 546, 579
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 2 cut(s) 64, 265
BarI GAAGNNNNNNTAC 2 cut(s) 736, 768
Bbv12I GWGCWC 1 cut(s) 447
BccI CCATC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BciT130I CCWGG 2 cut(s) 318, 709
BclI TGATCA 1 cut(s) 544
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 4 cut(s) 21, 93, 292, 326
BfmI CTRYAG 1 cut(s) 753
BfoI RGCGCY 2 cut(s) 68, 269
BisI GCNGC 2 cut(s) 264, 347
BlsI GCNGC 2 cut(s) 265, 348
Bme1390I CCNGG 3 cut(s) 318, 440, 709
BmeRI GACNNNNNGTC 2 cut(s) 34, 52
BmgBI CACGTC 2 cut(s) 33, 671
BmiI GGNNCC 3 cut(s) 66, 267, 666
BmrFI CCNGG 3 cut(s) 318, 440, 709
BmrI ACTGGG 1 cut(s) 242
BmuI ACTGGG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 440
BsaHI GRCGYC 3 cut(s) 65, 141, 266
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 269, 708
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse118I RCCGGY 2 cut(s) 61, 259
Bse1I ACTGG 2 cut(s) 248, 789
Bse3DI GCAATG 2 cut(s) 474, 767
BseBI CCWGG 2 cut(s) 318, 709
BseDI CCNNGG 2 cut(s) 269, 708
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMI GCAATG 2 cut(s) 474, 767
BseMII CTCAG 5 cut(s) 57, 66, 506, 585, 642
BseNI ACTGG 2 cut(s) 248, 789
BseRI GAGGAG 2 cut(s) 114, 310
BseSI GKGCMC 1 cut(s) 447
BshNI GGYRCC 2 cut(s) 64, 265
BsiHKAI GWGCWC 1 cut(s) 447
BsiSI CCGG 3 cut(s) 62, 260, 439
BslFI GGGAC 1 cut(s) 657
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 4 cut(s) 21, 93, 292, 326
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 657
Bso31I GGTCTC 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 3 cut(s) 435, 544, 664
BspACI CCGC 4 cut(s) 71, 119, 263, 347
BspCNI CTCAG 5 cut(s) 56, 67, 505, 586, 643
BspLI GGNNCC 3 cut(s) 66, 267, 666
BspPI GGATC 3 cut(s) 430, 659, 672
BspT107I GGYRCC 2 cut(s) 64, 265
BspTNI GGTCTC 1 cut(s) 326
BsrDI GCAATG 2 cut(s) 474, 767
BsrFI RCCGGY 2 cut(s) 61, 259
BsrI ACTGG 2 cut(s) 248, 789
BssAI RCCGGY 2 cut(s) 61, 259
BssECI CCNNGG 2 cut(s) 269, 708
BssMI GATC 3 cut(s) 435, 544, 664
BssNI GRCGYC 3 cut(s) 65, 141, 266
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 2 cut(s) 318, 709
Bst4CI ACNGT 4 cut(s) 303, 430, 757, 812
BstACI GRCGYC 3 cut(s) 65, 141, 266
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 5 cut(s) 43, 75, 492, 594, 651
BstH2I RGCGCY 2 cut(s) 68, 269
BstHHI GCGC 3 cut(s) 67, 182, 268
BstKTI GATC 3 cut(s) 438, 547, 667
BstMAI GTCTC 4 cut(s) 21, 93, 292, 326
BstMBI GATC 3 cut(s) 435, 544, 664
BstMWI GCNNNNNNNGC 2 cut(s) 14, 590
BstNI CCWGG 2 cut(s) 318, 709
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 3 cut(s) 316, 438, 707
BstSFI CTRYAG 1 cut(s) 753
BstSLI GKGCMC 1 cut(s) 447
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BtrI CACGTC 2 cut(s) 33, 671
BtsIMutI CAGTG 3 cut(s) 8, 573, 805
Cac8I GCNNGC 1 cut(s) 63
CfoI GCGC 3 cut(s) 67, 182, 268
Cfr10I RCCGGY 2 cut(s) 61, 259
CseI GACGC 1 cut(s) 149
CviAII CATG 2 cut(s) 236, 326
CviJI RGCY 8 cut(s) 17, 104, 187, 379, 484, 593, 617, 637
CviKI_1 RGCY 8 cut(s) 17, 104, 187, 379, 484, 593, 617, 637
DdeI CTNAG 5 cut(s) 43, 75, 492, 594, 651
DinI GGCGCC 2 cut(s) 66, 267
DpnI GATC 3 cut(s) 437, 546, 666
DpnII GATC 3 cut(s) 435, 544, 664
DriI GACNNNNNGTC 2 cut(s) 34, 52
Eam1105I GACNNNNNGTC 2 cut(s) 34, 52
EciI GGCGGA 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 326
Eco57I CTGAAG 2 cut(s) 470, 494
EcoRII CCWGG 2 cut(s) 316, 707
EcoT14I CCWWGG 1 cut(s) 269
EgeI GGCGCC 2 cut(s) 66, 267
EheI GGCGCC 2 cut(s) 66, 267
ErhI CCWWGG 1 cut(s) 269
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 2 cut(s) 239, 329
FaqI GGGAC 1 cut(s) 657
FatI CATG 2 cut(s) 235, 325
FbaI TGATCA 1 cut(s) 544
Fnu4HI GCNGC 2 cut(s) 264, 347
Fsp4HI GCNGC 2 cut(s) 264, 347
GlaI GCGC 3 cut(s) 66, 181, 267
GluI GCNGC 2 cut(s) 264, 347
HaeII RGCGCY 2 cut(s) 68, 269
HapII CCGG 3 cut(s) 62, 260, 439
HgaI GACGC 1 cut(s) 149
HhaI GCGC 3 cut(s) 67, 182, 268
Hin1I GRCGYC 3 cut(s) 65, 141, 266
Hin1II CATG 2 cut(s) 239, 329
Hin6I GCGC 3 cut(s) 65, 180, 266
HinP1I GCGC 3 cut(s) 65, 180, 266
HincII GTYRAC 1 cut(s) 538
HindII GTYRAC 1 cut(s) 538
HindIII AAGCTT 3 cut(s) 377, 482, 635
HinfI GANTC 9 cut(s) 41, 47, 53, 95, 455, 490, 610, 713, 795
HpaI GTTAAC 1 cut(s) 538
HpaII CCGG 3 cut(s) 62, 260, 439
HphI GGTGA 1 cut(s) 713
Hpy166II GTNNAC 3 cut(s) 334, 445, 538
Hpy188I TCNGA 6 cut(s) 40, 46, 52, 489, 495, 853
Hpy188III TCNNGA 3 cut(s) 92, 137, 164
Hpy8I GTNNAC 3 cut(s) 334, 445, 538
Hpy99I CGWCG 4 cut(s) 37, 40, 61, 143
HpyAV CCTTC 2 cut(s) 212, 268
HpyCH4III ACNGT 4 cut(s) 303, 430, 757, 812
HpyCH4IV ACGT 5 cut(s) 32, 86, 336, 354, 670
HpyCH4V TGCA 3 cut(s) 363, 445, 772
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 590
HpyF3I CTNAG 5 cut(s) 43, 75, 492, 594, 651
HpySE526I ACGT 5 cut(s) 32, 86, 336, 354, 670
Hsp92I GRCGYC 3 cut(s) 65, 141, 266
Hsp92II CATG 2 cut(s) 239, 329
HspAI GCGC 3 cut(s) 65, 180, 266
KasI GGCGCC 2 cut(s) 64, 265
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 544
KspAI GTTAAC 1 cut(s) 538
Kzo9I GATC 3 cut(s) 435, 544, 664
MaeII ACGT 5 cut(s) 32, 86, 336, 354, 670
MaeIII GTNAC 1 cut(s) 701
MalI GATC 3 cut(s) 437, 546, 666
MboI GATC 3 cut(s) 435, 544, 664
MboII GAAGA 4 cut(s) 101, 104, 746, 756
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 2 cut(s) 148, 845
Mly113I GGCGCC 2 cut(s) 65, 266
MlyI GAGTC 5 cut(s) 35, 41, 62, 449, 484
MmeI TCCRAC 1 cut(s) 18
MreI CGCCGGCG 1 cut(s) 61
MroNI GCCGGC 1 cut(s) 61
MseI TTAA 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 3 cut(s) 62, 260, 439
MspR9I CCNGG 3 cut(s) 318, 440, 709
MvaI CCWGG 2 cut(s) 318, 709
MwoI GCNNNNNNNGC 2 cut(s) 14, 590
NaeI GCCGGC 1 cut(s) 63
NarI GGCGCC 2 cut(s) 65, 266
NciI CCSGG 1 cut(s) 440
NdeII GATC 3 cut(s) 435, 544, 664
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 2 cut(s) 239, 329
NlaIV GGNNCC 3 cut(s) 66, 267, 666
NmeAIII GCCGAG 2 cut(s) 201, 213
NmuCI GTSAC 1 cut(s) 701
NspI RCATGY 1 cut(s) 239
PciI ACATGT 1 cut(s) 235
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 4 cut(s) 95, 610, 713, 795
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 2 cut(s) 265, 348
PleI GAGTC 5 cut(s) 35, 41, 61, 449, 484
PluTI GGCGCC 2 cut(s) 68, 269
PpsI GAGTC 5 cut(s) 35, 41, 61, 449, 484
PscI ACATGT 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 316, 707
PspGI CCWGG 2 cut(s) 316, 707
PspN4I GGNNCC 3 cut(s) 66, 267, 666
PsuI RGATCY 1 cut(s) 664
PvuII CAGCTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 2 cut(s) 264, 347
Sau3AI GATC 3 cut(s) 435, 544, 664
SchI GAGTC 5 cut(s) 35, 41, 62, 449, 484
ScrFI CCNGG 3 cut(s) 318, 440, 709
SduI GDGCHC 1 cut(s) 447
SfcI CTRYAG 1 cut(s) 753
SfoI GGCGCC 2 cut(s) 66, 267
SgrAI CRCCGGYG 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 2 cut(s) 148, 845
SsiI CCGC 4 cut(s) 71, 119, 263, 347
SspDI GGCGCC 2 cut(s) 64, 265
StyD4I CCNGG 3 cut(s) 316, 438, 707
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 4 cut(s) 303, 430, 757, 812
TaiI ACGT 5 cut(s) 35, 89, 339, 357, 673
TaqI TCGA 1 cut(s) 138
TasI AATT 2 cut(s) 148, 845
TauI GCSGC 2 cut(s) 266, 349
TfiI GAWTC 4 cut(s) 95, 610, 713, 795
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 3 cut(s) 15, 573, 805
TseFI GTSAC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 701
TspDTI ATGAA 4 cut(s) 141, 384, 398, 753
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 3 cut(s) 15, 573, 805
Van91I CCANNNNNTGG 1 cut(s) 331
VneI GTGCAC 1 cut(s) 443
XapI RAATTY 1 cut(s) 845
XceI RCATGY 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.