MD07G1252700.v1.1

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016155
Physical Location & Seq
Reverse (-)
32037822 .. 32040446
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1252700.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGTCTCTATATAAAAGGAGCCATATTTGCATTGAGAAACACGCAGAGAGAAGGGTGAGAGAGACACAGAGAGAAAGAAAGATGAGAAGCAACCAGGTTCGATTGAATTGTGGAATCACAATGCCTGTCATTGGGTTAGGCACTTATTCCTTCCAAAACGATAGGAAAACAACAGAACTTGCTGTCCATATGGCCCTCAAGGTACAAACCCTACACACACAAGAGAATTATGTCATCCCACCGGAAATATATCTTCTTATGTCTATAATACTTAGTACGGTAGTCACAAGGAGATATACTCTTGTGACACTACAGTTATCTTCTATCCGCTTGCTTTTATGTAACTTTTTTTACACAAGCGATATCGGGGAGGGACAAACTGAACTCAAGATTTCGGATATGGGTATGGGTTACAGGCATTTCGACACAGCGAAGATATATGGTTCGGAGCCGGCTCTCGGAAACGCTTTGACAGAGGCAATGCATGACGGAAAAGTAGAGAGGGAAGACATTTTTGTGACATCTAAGTTATGGGGTAGTGATCACCATGACCCTGTTGCAGGACTCAAGCAAACTCTCAAGAACATGGGAATGGAATATTTGGACATGTACCTAGTGCACTGGCCAGTGAAGTTGAAGCCATGGGCATGCAATCCCATTCCAGATGAAGATGAATTTGAGCCGTTGGATTTGGAGACCACTTGGTCTGGAATGCAGAAGTGCCTGGACTTGGGATTTTGCAGGTGCATTGGTGTCAGCAATTTCTCCACCAAAAAGATTGAACAGCTTCTGGATTTTGCCTCTGTTCCCCCAGCTGTCAATCAGGTCGAAATGCACCCGCTGTGGAGGCAAAGAAAGCTTCGAGATTTTTGTAGGGACCACAATATCCATGTAAGTGCTTACTCACCACTTGGTGGGCCTGGGAATTCATGGGGATCAACACTTGTGGTGAACAATCCAATCATCCGGGCCATTGCCAACAAGCGTAAAGCAACTCCAGCCCAGGTTGCTCTAAGGTGGGGATTATCAAAGGGATCAAGCATGATTGTGAAAAGCTTTAGTCCAGAAAGGATTAAGGAGAACTTTGGAGCCATTGATCTGAAATTGGACGGTCAAGATTTCATGGAGATAGACAAGTTGGAGGAAAGGAAGATCATGAGGGGAGAGTTTCTAGTCAATGAAACCACAAGCCCATACAAAACAATTCAAGATTTATGGGATGATGAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

411

Amino Acids

46.96

Weight (kDa)

7.18

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 136 - 381 4.8e-49 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62420
fragaria_vesca FvH4_7g27320
malus_domestica MD07G1252700.v1.1
prunus_persica Prupe.2G276200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374161
rosa_laevigata RLG00000026757
rosa_multiflora Rmu_ssc0000007.1_g000043 Rmu_ssc0000450.1_g000003
rosa_roxburghii Rroxscaffold_4G00283670
rosa_rugosa Rorug01G0381200
rosa_samantha Rh1AG392200 Rh1BG356200 Rh1CG369100 Rh1DG386700
rosa_wichuraiana Rw1G034350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 732
AasI GACNNNNNNGTC 1 cut(s) 703
Acc36I ACCTGC 1 cut(s) 732
AccB7I CCANNNNNTGG 1 cut(s) 914
AciI CCGC 2 cut(s) 328, 839
AclWI GGATC 2 cut(s) 943, 1042
AcoI YGGCCR 1 cut(s) 623
AcsI RAATTY 3 cut(s) 674, 925, 1227
AdeI CACNNNGTG 1 cut(s) 914
AfaI GTAC 3 cut(s) 204, 277, 611
AfiI CCNNNNNNNGG 5 cut(s) 131, 458, 560, 730, 914
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 4 cut(s) 106, 637, 782, 1208
AjnI CCWGG 4 cut(s) 93, 723, 919, 1002
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
AluBI AGCT 4 cut(s) 787, 815, 859, 1056
AluI AGCT 4 cut(s) 787, 815, 859, 1056
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 3 cut(s) 9, 56, 689
Alw44I GTGCAC 1 cut(s) 617
AlwI GGATC 2 cut(s) 943, 1042
AlwNI CAGNNNCTG 1 cut(s) 790
AoxI GGCC 4 cut(s) 192, 623, 917, 969
ApaLI GTGCAC 1 cut(s) 617
ApoI RAATTY 3 cut(s) 674, 925, 1227
Asp700I GAANNNNTTC 1 cut(s) 786
AspS9I GGNCC 4 cut(s) 193, 877, 917, 969
AsuC2I CCSGG 1 cut(s) 968
AsuHPI GGTGA 4 cut(s) 67, 536, 897, 961
AvaII GGWCC 1 cut(s) 877
BaeGI GKGCMC 1 cut(s) 621
BalI TGGCCA 1 cut(s) 625
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 621
BceAI ACGGC 1 cut(s) 667
BciT130I CCWGG 4 cut(s) 95, 725, 921, 1004
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 1 cut(s) 968
BcoDI GTCTC 3 cut(s) 9, 56, 689
BfaI CTAG 2 cut(s) 614, 1172
BfmI CTRYAG 1 cut(s) 311
BfuAI ACCTGC 1 cut(s) 732
Bme1390I CCNGG 5 cut(s) 95, 725, 921, 968, 1004
Bme18I GGWCC 1 cut(s) 877
BmgT120I GGNCC 4 cut(s) 193, 877, 917, 969
BmiI GGNNCC 4 cut(s) 20, 450, 878, 1090
BmrFI CCNGG 5 cut(s) 95, 725, 921, 968, 1004
BpiI GAAGAC 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 283, 315
BpmI CTGGAG 1 cut(s) 981
BpuEI CTTGAG 4 cut(s) 182, 371, 551, 563
BpuMI CCSGG 1 cut(s) 968
BsaI GGTCTC 1 cut(s) 689
BsaJI CCNNGG 3 cut(s) 641, 920, 1002
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 458, 560, 730, 914
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 626, 626
Bse3DI GCAATG 2 cut(s) 486, 972
BseBI CCWGG 4 cut(s) 95, 725, 921, 1004
BseDI CCNNGG 3 cut(s) 641, 920, 1002
BseGI GGATG 3 cut(s) 234, 963, 1225
BseLI CCNNNNNNNGG 5 cut(s) 131, 458, 560, 730, 914
BseMI GCAATG 2 cut(s) 486, 972
BseNI ACTGG 2 cut(s) 626, 626
BseSI GKGCMC 1 cut(s) 621
BseYI CCCAGC 1 cut(s) 811
BshFI GGCC 4 cut(s) 194, 625, 919, 971
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 3 cut(s) 242, 452, 967
BslFI GGGAC 2 cut(s) 387, 890
BslI CCNNNNNNNGG 5 cut(s) 131, 458, 560, 730, 914
BsmAI GTCTC 3 cut(s) 9, 56, 689
BsmFI GGGAC 2 cut(s) 387, 890
BsmI GAATGC 1 cut(s) 717
BsnI GGCC 4 cut(s) 194, 625, 919, 971
Bso31I GGTCTC 1 cut(s) 689
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 5 cut(s) 541, 935, 1034, 1096, 1152
Bsp19I CCATGG 1 cut(s) 641
BspACI CCGC 2 cut(s) 328, 839
BspANI GGCC 4 cut(s) 194, 625, 919, 971
BspHI TCATGA 1 cut(s) 1155
BspLI GGNNCC 4 cut(s) 20, 450, 878, 1090
BspMI ACCTGC 1 cut(s) 732
BspPI GGATC 2 cut(s) 943, 1042
BspTNI GGTCTC 1 cut(s) 689
BsrDI GCAATG 2 cut(s) 486, 972
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 2 cut(s) 626, 626
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 3 cut(s) 641, 920, 1002
BssMI GATC 5 cut(s) 541, 935, 1034, 1096, 1152
BssT1I CCWWGG 1 cut(s) 641
Bst2UI CCWGG 4 cut(s) 95, 725, 921, 1004
Bst4CI ACNGT 3 cut(s) 280, 315, 1112
BstC8I GCNNGC 3 cut(s) 332, 453, 649
BstDEI CTNAG 3 cut(s) 272, 525, 1013
BstDSI CCRYGG 1 cut(s) 641
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 3 cut(s) 234, 963, 1225
BstKTI GATC 5 cut(s) 544, 938, 1037, 1099, 1155
BstMAI GTCTC 3 cut(s) 9, 56, 689
BstMBI GATC 5 cut(s) 541, 935, 1034, 1096, 1152
BstMWI GCNNNNNNNGC 5 cut(s) 27, 847, 856, 998, 1007
BstNI CCWGG 4 cut(s) 95, 725, 921, 1004
BstNSI RCATGY 2 cut(s) 610, 651
BstSCI CCNGG 5 cut(s) 93, 723, 919, 966, 1002
BstSFI CTRYAG 1 cut(s) 311
BstSLI GKGCMC 1 cut(s) 621
BstV2I GAAGAC 1 cut(s) 513
BsuRI GGCC 4 cut(s) 194, 625, 919, 971
BtgI CCRYGG 1 cut(s) 641
BtsCI GGATG 3 cut(s) 234, 963, 1225
BtsIMutI CAGTG 2 cut(s) 619, 633
BveI ACCTGC 1 cut(s) 732
Cac8I GCNNGC 3 cut(s) 332, 453, 649
CaiI CAGNNNCTG 1 cut(s) 790
CciI TCATGA 1 cut(s) 1155
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 4 cut(s) 193, 877, 917, 969
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 3 cut(s) 203, 276, 610
CspCI CAANNNNNGTGG 2 cut(s) 927, 962
CviQI GTAC 3 cut(s) 203, 276, 610
DdeI CTNAG 3 cut(s) 272, 525, 1013
DpnI GATC 5 cut(s) 543, 937, 1036, 1098, 1154
DpnII GATC 5 cut(s) 541, 935, 1034, 1096, 1152
DraIII CACNNNGTG 1 cut(s) 914
DrdI GACNNNNNNGTC 1 cut(s) 703
DseDI GACNNNNNNGTC 1 cut(s) 703
EaeI YGGCCR 1 cut(s) 623
Eco130I CCWWGG 1 cut(s) 641
Eco31I GGTCTC 1 cut(s) 689
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 877
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoRI GAATTC 1 cut(s) 925
EcoRII CCWGG 4 cut(s) 93, 723, 919, 1002
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 641
EcoT22I ATGCAT 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 641
FalI AAGNNNNNCTT 2 cut(s) 1067, 1099
FaqI GGGAC 2 cut(s) 387, 890
FauI CCCGC 1 cut(s) 846
FauNDI CATATG 1 cut(s) 189
FbaI TGATCA 1 cut(s) 541
FokI GGATG 2 cut(s) 221, 950
FspBI CTAG 2 cut(s) 614, 1172
GsaI CCCAGC 1 cut(s) 815
GsuI CTGGAG 1 cut(s) 981
HaeIII GGCC 4 cut(s) 194, 625, 919, 971
HapII CCGG 3 cut(s) 242, 452, 967
HindIII AAGCTT 2 cut(s) 857, 1054
HinfI GANTC 2 cut(s) 114, 564
HpaII CCGG 3 cut(s) 242, 452, 967
HphI GGTGA 4 cut(s) 67, 536, 897, 961
Hpy166II GTNNAC 2 cut(s) 619, 952
Hpy188I TCNGA 4 cut(s) 397, 448, 461, 1101
Hpy8I GTNNAC 2 cut(s) 619, 952
HpyAV CCTTC 2 cut(s) 45, 160
HpyCH4III ACNGT 3 cut(s) 280, 315, 1112
HpyCH4V TGCA 9 cut(s) 30, 484, 560, 619, 651, 715, 741, 747, 835
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 847, 856, 998, 1007
HpyF3I CTNAG 3 cut(s) 272, 525, 1013
KroI GCCGGC 1 cut(s) 451
KroNI GCCGGC 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 5 cut(s) 541, 935, 1034, 1096, 1152
LmnI GCTCC 3 cut(s) 18, 448, 1088
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 2 cut(s) 614, 1172
MaeIII GTNAC 5 cut(s) 283, 304, 341, 410, 517
MalI GATC 5 cut(s) 543, 937, 1036, 1098, 1154
MboI GATC 5 cut(s) 541, 935, 1034, 1096, 1152
MboII GAAGA 6 cut(s) 245, 312, 445, 518, 680, 1162
MhlI GDGCHC 1 cut(s) 621
MlsI TGGCCA 1 cut(s) 625
MluCI AATT 8 cut(s) 106, 226, 674, 760, 925, 1103, 1203, 1227
MluNI TGGCCA 1 cut(s) 625
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 2 cut(s) 666, 1119
MnlI CCTC 8 cut(s) 206, 364, 469, 495, 811, 840, 1135, 1152
Mox20I TGGCCA 1 cut(s) 625
Mph1103I ATGCAT 1 cut(s) 486
MroNI GCCGGC 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 625
MseI TTAA 2 cut(s) 1074, 1231
MslI CAYNNNNRTG 5 cut(s) 515, 590, 646, 894, 1046
Msp20I TGGCCA 1 cut(s) 625
MspA1I CMGCKG 2 cut(s) 815, 841
MspI CCGG 3 cut(s) 242, 452, 967
MspR9I CCNGG 5 cut(s) 95, 725, 921, 968, 1004
Mva1269I GAATGC 1 cut(s) 717
MvaI CCWGG 4 cut(s) 95, 725, 921, 1004
MwoI GCNNNNNNNGC 5 cut(s) 27, 847, 856, 998, 1007
NaeI GCCGGC 1 cut(s) 453
NciI CCSGG 1 cut(s) 968
NcoI CCATGG 1 cut(s) 641
NdeI CATATG 1 cut(s) 189
NdeII GATC 5 cut(s) 541, 935, 1034, 1096, 1152
NgoMIV GCCGGC 1 cut(s) 451
NlaIV GGNNCC 4 cut(s) 20, 450, 878, 1090
NmuCI GTSAC 3 cut(s) 283, 304, 517
NsiI ATGCAT 1 cut(s) 486
NspI RCATGY 2 cut(s) 610, 651
PaeI GCATGC 1 cut(s) 651
PagI TCATGA 1 cut(s) 1155
PaqCI CACCTGC 1 cut(s) 732
PciI ACATGT 1 cut(s) 606
PctI GAATGC 1 cut(s) 717
PdiI GCCGGC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 1 cut(s) 114
PflMI CCANNNNNTGG 1 cut(s) 914
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
PscI ACATGT 1 cut(s) 606
Psp6I CCWGG 4 cut(s) 93, 723, 919, 1002
PspFI CCCAGC 1 cut(s) 811
PspGI CCWGG 4 cut(s) 93, 723, 919, 1002
PspN4I GGNNCC 4 cut(s) 20, 450, 878, 1090
PspPI GGNCC 4 cut(s) 193, 877, 917, 969
PstNI CAGNNNCTG 1 cut(s) 790
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 3 cut(s) 204, 277, 611
RsaNI GTAC 3 cut(s) 203, 276, 610
RseI CAYNNNNRTG 5 cut(s) 515, 590, 646, 894, 1046
SaqAI TTAA 2 cut(s) 1074, 1231
Sau3AI GATC 5 cut(s) 541, 935, 1034, 1096, 1152
Sau96I GGNCC 4 cut(s) 193, 877, 917, 969
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 5 cut(s) 95, 725, 921, 968, 1004
SduI GDGCHC 1 cut(s) 621
SexAI ACCWGGT 1 cut(s) 93
SfcI CTRYAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 877
SmiMI CAYNNNNRTG 5 cut(s) 515, 590, 646, 894, 1046
SmlI CTYRAG 4 cut(s) 197, 386, 566, 578
SmoI CTYRAG 4 cut(s) 197, 386, 566, 578
SphI GCATGC 1 cut(s) 651
Sse9I AATT 8 cut(s) 106, 226, 674, 760, 925, 1103, 1203, 1227
SsiI CCGC 2 cut(s) 328, 839
SspI AATATT 1 cut(s) 599
SspMI CTAG 2 cut(s) 614, 1172
StyD4I CCNGG 5 cut(s) 93, 723, 919, 966, 1002
StyI CCWWGG 1 cut(s) 641
TaaI ACNGT 3 cut(s) 280, 315, 1112
TaqI TCGA 4 cut(s) 100, 423, 828, 862
TasI AATT 8 cut(s) 106, 226, 674, 760, 925, 1103, 1203, 1227
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 2 cut(s) 1074, 1231
Tru9I TTAA 2 cut(s) 1074, 1231
TscAI CASTG 2 cut(s) 626, 633
TseFI GTSAC 3 cut(s) 283, 304, 517
Tsp45I GTSAC 3 cut(s) 283, 304, 517
TspDTI ATGAA 5 cut(s) 681, 687, 918, 1111, 1194
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 2 cut(s) 626, 633
Van91I CCANNNNNTGG 1 cut(s) 914
VneI GTGCAC 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 877
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 3 cut(s) 674, 925, 1227
XceI RCATGY 2 cut(s) 610, 651
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 2 cut(s) 614, 1172
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.