Rmu_ssc0000450.1_g000003

Aldo-keto reductase family 4 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000450.1
OG0016155
Physical Location & Seq
Reverse (-)
16747 .. 19630
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000450.1_g000003.1.cds

Sequence Viewer

Length: 858 bp
atggtcgacgcgccaaatggagggagtggaggactgggcatcgacgcgtggcgcctcaaaggcagctcgattgacgccacagcgctgccggaagatttgctccgcctgggctgcttcgatctcggcctgggggaaacttttgggcggtttgactccacagagctgcaggacgaggtgaggcgacctcgggggtggccgggtccttggccggaggacgccggaggacagagaaaaatccggggcgggtcggacgaaaaacaactgatgggctacaggcactttgacacagccaggatatatggttctgaggctgccctgggaaatgctttaacagaggcaattcaggatgggactgtagaaagagaagacatttttgttacctctaagttatggggtagtcatcaccatgaccctgtttcagctctgagacaaactctaaagactacgggaatggaccatttggacatgtacttggttcactggccagtgaaattgaagccatgggcttctgatgccattccgaaagaggatcactttgaggaactagacatagagacaacctgggctggtatggagaagtgcctggacttgggattgtgtaggtctattggtgtaagcaatttctcaagcaaaaaaattgaacagcttttggaatttgcctctgtgcctccagctgttaatcaggtggagatgcacccaatgtggaggcaagcaagactgagaagggtgtgtggggaccacaaaatccatgtcagtgcttactcaccacttgtaatcaactcttcagaggagctgaagtctcgtgcccatatcggagtggaggccagtggtgcactgaatcggataagaggagagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00040 ko00561 ko00930 ko01100 ko01110 ko01120 ko01130 ko01220 map00010 map00040 map00561 map00930 map01100 map01110 map01120 map01130 map01220
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

285

Amino Acids

31.61

Weight (kDa)

5.69

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62420
fragaria_vesca FvH4_7g27320
malus_domestica MD07G1252700.v1.1
prunus_persica Prupe.2G276200_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0374161
rosa_laevigata RLG00000026757
rosa_multiflora Rmu_ssc0000007.1_g000043 Rmu_ssc0000450.1_g000003
rosa_roxburghii Rroxscaffold_4G00283670
rosa_rugosa Rorug01G0381200
rosa_samantha Rh1AG392200 Rh1BG356200 Rh1CG369100 Rh1DG386700
rosa_wichuraiana Rw1G034350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 6
AccII CGCG 2 cut(s) 11, 47
AciI CCGC 3 cut(s) 103, 145, 243
AclWI GGATC 1 cut(s) 537
AcoI YGGCCR 3 cut(s) 194, 206, 482
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 2 cut(s) 768, 815
AcyI GRCGYC 3 cut(s) 52, 75, 216
AfaI GTAC 1 cut(s) 470
AfeI AGCGCT 1 cut(s) 84
AfiI CCNNNNNNNGG 2 cut(s) 20, 589
AflIII ACRYGT 2 cut(s) 45, 465
AgsI TTSAA 2 cut(s) 496, 641
AjnI CCWGG 6 cut(s) 105, 126, 290, 315, 560, 582
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 6 cut(s) 66, 163, 422, 646, 674, 793
AluI AGCT 6 cut(s) 66, 163, 422, 646, 674, 793
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 3 cut(s) 421, 548, 804
Alw44I GTGCAC 1 cut(s) 831
AlwI GGATC 1 cut(s) 537
Ama87I CYCGRG 1 cut(s) 186
Aor51HI AGCGCT 1 cut(s) 84
AoxI GGCC 5 cut(s) 124, 194, 206, 482, 822
ApaLI GTGCAC 1 cut(s) 831
ApeKI GCWGC 5 cut(s) 63, 85, 111, 163, 311
ApoI RAATTY 1 cut(s) 653
ArsI GACNNNNNNTTYG 2 cut(s) 735, 767
AspLEI GCGC 3 cut(s) 13, 54, 85
AspS9I GGNCC 3 cut(s) 200, 454, 736
AsuC2I CCSGG 2 cut(s) 198, 239
AsuHPI GGTGA 3 cut(s) 187, 395, 756
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 3 cut(s) 200, 454, 736
BaeGI GKGCMC 2 cut(s) 808, 835
BalI TGGCCA 1 cut(s) 484
BanI GGYRCC 1 cut(s) 51
BauI CACGAG 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 1 cut(s) 835
BbvI GCAGC 5 cut(s) 72, 75, 98, 150, 298
BccI CCATC 2 cut(s) 259, 341
BciT130I CCWGG 6 cut(s) 107, 128, 292, 317, 562, 584
BcnI CCSGG 2 cut(s) 198, 239
BcoDI GTCTC 3 cut(s) 421, 548, 804
BfaI CTAG 1 cut(s) 545
BfmI CTRYAG 3 cut(s) 164, 271, 354
BfoI RGCGCY 2 cut(s) 55, 86
BglI GCCNNNNNGGC 1 cut(s) 60
BisI GCNGC 5 cut(s) 64, 86, 112, 164, 312
BlsI GCNGC 5 cut(s) 65, 87, 113, 165, 313
Bme1390I CCNGG 8 cut(s) 107, 128, 198, 239, 292, 317, 562, 584
Bme18I GGWCC 3 cut(s) 200, 454, 736
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 200, 454, 736
BmiI GGNNCC 3 cut(s) 53, 201, 737
BmrFI CCNGG 8 cut(s) 107, 128, 198, 239, 292, 317, 562, 584
BmrI ACTGGG 1 cut(s) 44
BmsI GCATC 3 cut(s) 48, 502, 681
BmuI ACTGGG 1 cut(s) 44
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 4 cut(s) 169, 201, 418, 450
BpmI CTGGAG 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 610
BpuMI CCSGG 2 cut(s) 198, 239
BsaHI GRCGYC 3 cut(s) 52, 75, 216
BsaJI CCNNGG 9 cut(s) 106, 127, 185, 203, 238, 315, 316, 500, 561
BsaXI ACNNNNNCTCC 2 cut(s) 782, 812
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 589
Bse1I ACTGG 4 cut(s) 39, 485, 485, 825
BseBI CCWGG 6 cut(s) 107, 128, 292, 317, 562, 584
BseDI CCNNGG 9 cut(s) 106, 127, 185, 203, 238, 315, 316, 500, 561
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 2 cut(s) 20, 589
BseMII CTCAG 3 cut(s) 297, 416, 710
BseNI ACTGG 4 cut(s) 39, 485, 485, 825
BseRI GAGGAG 1 cut(s) 803
BseSI GKGCMC 2 cut(s) 808, 835
BseXI GCAGC 5 cut(s) 72, 75, 98, 150, 298
Bsh1236I CGCG 2 cut(s) 11, 47
BshFI GGCC 5 cut(s) 126, 196, 208, 484, 824
BshNI GGYRCC 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 835
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 5 cut(s) 89, 197, 209, 219, 238
BslFI GGGAC 2 cut(s) 364, 749
BslI CCNNNNNNNGG 2 cut(s) 20, 589
BsmAI GTCTC 3 cut(s) 421, 548, 804
BsmFI GGGAC 2 cut(s) 364, 749
BsnI GGCC 5 cut(s) 126, 196, 208, 484, 824
BsoBI CYCGRG 1 cut(s) 186
Bsp1286I GDGCHC 2 cut(s) 808, 835
Bsp143I GATC 2 cut(s) 118, 529
Bsp19I CCATGG 1 cut(s) 500
BspACI CCGC 3 cut(s) 103, 145, 243
BspANI GGCC 5 cut(s) 126, 196, 208, 484, 824
BspCNI CTCAG 3 cut(s) 298, 417, 711
BspFNI CGCG 2 cut(s) 11, 47
BspLI GGNNCC 3 cut(s) 53, 201, 737
BspMAI CTGCAG 1 cut(s) 168
BspPI GGATC 1 cut(s) 537
BspT107I GGYRCC 1 cut(s) 51
BsrI ACTGG 4 cut(s) 39, 485, 485, 825
BssECI CCNNGG 9 cut(s) 106, 127, 185, 203, 238, 315, 316, 500, 561
BssMI GATC 2 cut(s) 118, 529
BssNI GRCGYC 3 cut(s) 52, 75, 216
BssSI CACGAG 1 cut(s) 801
BssT1I CCWWGG 2 cut(s) 203, 500
Bst2BI CACGAG 1 cut(s) 801
Bst2UI CCWGG 6 cut(s) 107, 128, 292, 317, 562, 584
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 787
BstACI GRCGYC 3 cut(s) 52, 75, 216
BstC8I GCNNGC 1 cut(s) 711
BstDEI CTNAG 4 cut(s) 306, 384, 425, 719
BstDSI CCRYGG 1 cut(s) 500
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 2 cut(s) 11, 47
BstH2I RGCGCY 2 cut(s) 55, 86
BstHHI GCGC 3 cut(s) 13, 54, 85
BstKTI GATC 2 cut(s) 121, 532
BstMAI GTCTC 3 cut(s) 421, 548, 804
BstMBI GATC 2 cut(s) 118, 529
BstMWI GCNNNNNNNGC 4 cut(s) 60, 111, 512, 830
BstNI CCWGG 6 cut(s) 107, 128, 292, 317, 562, 584
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 8 cut(s) 105, 126, 196, 237, 290, 315, 560, 582
BstSFI CTRYAG 3 cut(s) 164, 271, 354
BstSLI GKGCMC 2 cut(s) 808, 835
BstUI CGCG 2 cut(s) 11, 47
BstV1I GCAGC 5 cut(s) 72, 75, 98, 150, 298
BstV2I GAAGAC 1 cut(s) 372
BsuRI GGCC 5 cut(s) 126, 196, 208, 484, 824
BtgI CCRYGG 1 cut(s) 500
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 5 cut(s) 478, 492, 760, 832, 833
Cac8I GCNNGC 1 cut(s) 711
CfoI GCGC 3 cut(s) 13, 54, 85
Cfr13I GGNCC 3 cut(s) 200, 454, 736
CseI GACGC 4 cut(s) 17, 53, 83, 224
Csp6I GTAC 1 cut(s) 469
CviAII CATG 4 cut(s) 407, 466, 501, 749
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 4 cut(s) 306, 384, 425, 719
DinI GGCGCC 1 cut(s) 53
DpnI GATC 2 cut(s) 120, 531
DpnII GATC 2 cut(s) 118, 529
EaeI YGGCCR 3 cut(s) 194, 206, 482
Eam1104I CTCTTC 1 cut(s) 787
EarI CTCTTC 1 cut(s) 787
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 2 cut(s) 203, 500
Eco47I GGWCC 3 cut(s) 200, 454, 736
Eco47III AGCGCT 1 cut(s) 84
Eco57I CTGAAG 2 cut(s) 768, 815
Eco88I CYCGRG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 200
EcoRII CCWGG 6 cut(s) 105, 126, 290, 315, 560, 582
EcoT14I CCWWGG 2 cut(s) 203, 500
EgeI GGCGCC 1 cut(s) 53
EheI GGCGCC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 203, 500
FaeI CATG 4 cut(s) 410, 469, 504, 752
FaqI GGGAC 2 cut(s) 364, 749
FatI CATG 4 cut(s) 406, 465, 500, 748
FauI CCCGC 1 cut(s) 236
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 5 cut(s) 64, 86, 112, 164, 312
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 5 cut(s) 64, 86, 112, 164, 312
FspBI CTAG 1 cut(s) 545
GlaI GCGC 3 cut(s) 12, 53, 84
GluI GCNGC 5 cut(s) 64, 86, 112, 164, 312
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 2 cut(s) 55, 86
HaeIII GGCC 5 cut(s) 126, 196, 208, 484, 824
HapII CCGG 5 cut(s) 89, 197, 209, 219, 238
HgaI GACGC 4 cut(s) 17, 53, 83, 224
HhaI GCGC 3 cut(s) 13, 54, 85
Hin1I GRCGYC 3 cut(s) 52, 75, 216
Hin1II CATG 4 cut(s) 410, 469, 504, 752
Hin6I GCGC 3 cut(s) 11, 52, 83
HinP1I GCGC 3 cut(s) 11, 52, 83
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 2 cut(s) 152, 838
HpaII CCGG 5 cut(s) 89, 197, 209, 219, 238
HphI GGTGA 3 cut(s) 187, 395, 756
Hpy166II GTNNAC 3 cut(s) 7, 478, 833
Hpy188I TCNGA 8 cut(s) 250, 307, 426, 511, 522, 787, 815, 843
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 3 cut(s) 7, 478, 833
Hpy99I CGWCG 2 cut(s) 11, 47
HpyAV CCTTC 1 cut(s) 717
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4V TGCA 3 cut(s) 166, 694, 833
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 111, 512, 830
HpyF3I CTNAG 4 cut(s) 306, 384, 425, 719
Hsp92I GRCGYC 3 cut(s) 52, 75, 216
Hsp92II CATG 4 cut(s) 410, 469, 504, 752
HspAI GCGC 3 cut(s) 11, 52, 83
KasI GGCGCC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 118, 529
LmnI GCTCC 2 cut(s) 105, 790
Lsp1109I GCAGC 5 cut(s) 72, 75, 98, 150, 298
LweI GCATC 3 cut(s) 48, 502, 681
MaeI CTAG 1 cut(s) 545
MaeIII GTNAC 1 cut(s) 376
MalI GATC 2 cut(s) 120, 531
MboI GATC 2 cut(s) 118, 529
MboII GAAGA 3 cut(s) 104, 377, 774
MhlI GDGCHC 2 cut(s) 808, 835
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 5 cut(s) 339, 491, 619, 636, 653
MluI ACGCGT 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 484
Mly113I GGCGCC 1 cut(s) 52
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 1 cut(s) 228
Mox20I TGGCCA 1 cut(s) 484
MscI TGGCCA 1 cut(s) 484
MseI TTAA 2 cut(s) 329, 678
MslI CAYNNNNRTG 2 cut(s) 405, 753
Msp20I TGGCCA 1 cut(s) 484
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 5 cut(s) 89, 197, 209, 219, 238
MspR9I CCNGG 8 cut(s) 107, 128, 198, 239, 292, 317, 562, 584
MvaI CCWGG 6 cut(s) 107, 128, 292, 317, 562, 584
MvnI CGCG 2 cut(s) 11, 47
MwoI GCNNNNNNNGC 4 cut(s) 60, 111, 512, 830
NarI GGCGCC 1 cut(s) 52
NciI CCSGG 2 cut(s) 198, 239
NcoI CCATGG 1 cut(s) 500
NdeII GATC 2 cut(s) 118, 529
NlaIII CATG 4 cut(s) 410, 469, 504, 752
NlaIV GGNNCC 3 cut(s) 53, 201, 737
NmeAIII GCCGAG 1 cut(s) 102
NspI RCATGY 1 cut(s) 469
PasI CCCWGGG 1 cut(s) 316
PciI ACATGT 1 cut(s) 465
PfeI GAWTC 1 cut(s) 838
PkrI GCNGC 5 cut(s) 65, 87, 113, 165, 313
PleI GAGTC 1 cut(s) 146
PluTI GGCGCC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 200
PscI ACATGT 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 200
Psp6I CCWGG 6 cut(s) 105, 126, 290, 315, 560, 582
PspGI CCWGG 6 cut(s) 105, 126, 290, 315, 560, 582
PspN4I GGNNCC 3 cut(s) 53, 201, 737
PspPI GGNCC 3 cut(s) 200, 454, 736
PspPPI RGGWCCY 1 cut(s) 200
PstI CTGCAG 1 cut(s) 168
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
RseI CAYNNNNRTG 2 cut(s) 405, 753
SalI GTCGAC 1 cut(s) 5
SaqAI TTAA 2 cut(s) 329, 678
SatI GCNGC 5 cut(s) 64, 86, 112, 164, 312
Sau3AI GATC 2 cut(s) 118, 529
Sau96I GGNCC 3 cut(s) 200, 454, 736
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 8 cut(s) 107, 128, 198, 239, 292, 317, 562, 584
SduI GDGCHC 2 cut(s) 808, 835
SfaNI GCATC 3 cut(s) 48, 502, 681
SfcI CTRYAG 3 cut(s) 164, 271, 354
SfoI GGCGCC 1 cut(s) 53
SinI GGWCC 3 cut(s) 200, 454, 736
SmiMI CAYNNNNRTG 2 cut(s) 405, 753
SmlI CTYRAG 1 cut(s) 625
SmoI CTYRAG 1 cut(s) 625
Sse9I AATT 5 cut(s) 339, 491, 619, 636, 653
SsiI CCGC 3 cut(s) 103, 145, 243
SspDI GGCGCC 1 cut(s) 51
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 8 cut(s) 105, 126, 196, 237, 290, 315, 560, 582
StyI CCWWGG 2 cut(s) 203, 500
TaaI ACNGT 1 cut(s) 355
TaqI TCGA 4 cut(s) 6, 42, 68, 117
TasI AATT 5 cut(s) 339, 491, 619, 636, 653
TatI WGTACW 1 cut(s) 468
TfiI GAWTC 1 cut(s) 838
Tru1I TTAA 2 cut(s) 329, 678
Tru9I TTAA 2 cut(s) 329, 678
TscAI CASTG 5 cut(s) 485, 492, 760, 832, 840
TseI GCWGC 5 cut(s) 63, 85, 111, 163, 311
TspRI CASTG 5 cut(s) 485, 492, 760, 832, 840
VneI GTGCAC 1 cut(s) 831
VpaK11BI GGWCC 3 cut(s) 200, 454, 736
XapI RAATTY 1 cut(s) 653
XceI RCATGY 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 6
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.