MD07G1298500.v1.1

translational elongation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005247
Physical Location & Seq
Forward (+)
35804343 .. 35805276
934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1298500.v1.1.491

Sequence Viewer

Length: 369 bp
ATGTCGACCGCCGAGCTTGCTTACTCCTACGCAGCCATGATCCTTCAGGACGATGGCATGTCGATCACTGCTGAGAAGATCGGCGCCTTGGTGAAATCTGCTAATGTGTCAGTGGAGTCATGCTGGCTTGGCCTCTTTGCCAGGCTCGCAGAAAAGAGGAACCTGGAGGATCTTTTTTTGAATGCTTGCGCAACCGGTGGTTCACCTCCGGTAGCCGTGCCCCCTTCTGCTGCTGGTGCAGGTGGTGCTTCCAGCGCCGCTGCCCAACTTTGTCAAGGGGATGATTTTCGGTTGCTGATATTGTGCCCGCTACTTCGATTGCGACTGCCTTTACCCAGGGCGGTAGCGTTGGCTCTGGCTATGACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000322 GO:0000323 GO:0000324 GO:0000956 GO:0001932 GO:0001934 GO:0002181 GO:0002182 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0005840 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006414 GO:0006417 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0008047 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009719 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010033 GO:0010243 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0014070 GO:0014074 GO:0015031 GO:0015833 GO:0015934 GO:0016070 GO:0016071 GO:0019207 GO:0019209 GO:0019220 GO:0019222 GO:0019439 GO:0019538 GO:0019887 GO:0022625 GO:0022626 GO:0030234 GO:0030295 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032991 GO:0033036 GO:0033365 GO:0033674 GO:0034248 GO:0034250 GO:0034613 GO:0034641 GO:0034645 GO:0034655 GO:0042221 GO:0042325 GO:0042327 GO:0042886 GO:0043021 GO:0043043 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044877 GO:0045047 GO:0045184 GO:0045727 GO:0045859 GO:0045860 GO:0045937 GO:0046483 GO:0046683 GO:0046700 GO:0046907 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051591 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0065009 GO:0070727 GO:0070887 GO:0070972 GO:0071310 GO:0071320 GO:0071407 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0072657 GO:0080090 GO:0090150 GO:0090304 GO:0098772 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1904400 GO:1904401 GO:1904587 GO:1904588 GO:1990904 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 ko01110 ko03010 ko05168 map00564 map01100 map01110 map03010 map05168
Pfam Domains
Protein Families
Ribosomal_60s

Protein Analysis

123

Amino Acids

12.59

Weight (kDa)

5.24

Isoelectric Point (pI)

59.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_60s PF00428 21 - 88 2.3e-11 60s Acidic ribosomal protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 230
AccB1I GGYRCC 1 cut(s) 83
AccI GTMKAC 1 cut(s) 5
AciI CCGC 4 cut(s) 9, 258, 308, 341
AclWI GGATC 2 cut(s) 34, 177
AcuI CTGAAG 1 cut(s) 29
AcyI GRCGYC 1 cut(s) 84
AgeI ACCGGT 1 cut(s) 194
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 3 cut(s) 140, 162, 335
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
AlwI GGATC 2 cut(s) 34, 177
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 3 cut(s) 32, 230, 260
AsiGI ACCGGT 1 cut(s) 194
AspLEI GCGC 3 cut(s) 86, 191, 257
AsuHPI GGTGA 2 cut(s) 103, 195
BaeGI GKGCMC 2 cut(s) 222, 308
BanI GGYRCC 1 cut(s) 83
BbvI GCAGC 3 cut(s) 44, 217, 247
BccI CCATC 1 cut(s) 47
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 3 cut(s) 142, 164, 337
BfoI RGCGCY 2 cut(s) 87, 258
BfuAI ACCTGC 1 cut(s) 230
BisI GCNGC 4 cut(s) 33, 231, 258, 261
BlsI GCNGC 4 cut(s) 34, 232, 259, 262
Bme1390I CCNGG 3 cut(s) 142, 164, 337
BmiI GGNNCC 2 cut(s) 85, 161
BmrFI CCNGG 3 cut(s) 142, 164, 337
BpmI CTGGAG 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 87, 335, 336
BsaWI WCCGGW 2 cut(s) 194, 208
Bse118I RCCGGY 1 cut(s) 194
BseBI CCWGG 3 cut(s) 142, 164, 337
BseDI CCNNGG 3 cut(s) 87, 335, 336
BseGI GGATG 1 cut(s) 286
BseMII CTCAG 1 cut(s) 63
BseSI GKGCMC 2 cut(s) 222, 308
BseXI GCAGC 3 cut(s) 44, 217, 247
BsgI GTGCAG 1 cut(s) 258
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 1 cut(s) 83
BshTI ACCGGT 1 cut(s) 194
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 195, 209
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 222, 308
Bsp143I GATC 4 cut(s) 39, 63, 78, 169
BspACI CCGC 4 cut(s) 9, 258, 308, 341
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 85, 161
BspMI ACCTGC 1 cut(s) 230
BspPI GGATC 2 cut(s) 34, 177
BspT107I GGYRCC 1 cut(s) 83
BsrFI RCCGGY 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 3 cut(s) 87, 335, 336
BssMI GATC 4 cut(s) 39, 63, 78, 169
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 142, 164, 337
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 245
BstC8I GCNNGC 5 cut(s) 18, 125, 147, 187, 308
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 286
BstH2I RGCGCY 2 cut(s) 87, 258
BstHHI GCGC 3 cut(s) 86, 191, 257
BstKTI GATC 4 cut(s) 42, 66, 81, 172
BstMBI GATC 4 cut(s) 39, 63, 78, 169
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 6 cut(s) 17, 129, 146, 236, 245, 254
BstNI CCWGG 3 cut(s) 142, 164, 337
BstNSI RCATGY 1 cut(s) 61
BstSCI CCNGG 3 cut(s) 140, 162, 335
BstSLI GKGCMC 2 cut(s) 222, 308
BstV1I GCAGC 3 cut(s) 44, 217, 247
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 117
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 5 cut(s) 18, 125, 147, 187, 308
CfoI GCGC 3 cut(s) 86, 191, 257
Cfr10I RCCGGY 1 cut(s) 194
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 3 cut(s) 37, 58, 120
CviJI RGCY 8 cut(s) 16, 35, 127, 132, 145, 215, 353, 359
CviKI_1 RGCY 8 cut(s) 16, 35, 127, 132, 145, 215, 353, 359
DdeI CTNAG 1 cut(s) 72
DinI GGCGCC 1 cut(s) 85
DpnI GATC 4 cut(s) 41, 65, 80, 171
DpnII GATC 4 cut(s) 39, 63, 78, 169
Eco130I CCWWGG 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 29
EcoRII CCWGG 3 cut(s) 140, 162, 335
EcoT14I CCWWGG 1 cut(s) 87
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 3 cut(s) 40, 61, 123
FaiI YATR 4 cut(s) 38, 59, 121, 362
FatI CATG 3 cut(s) 36, 57, 119
FauI CCCGC 1 cut(s) 315
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 33, 231, 258, 261
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 4 cut(s) 33, 231, 258, 261
FspI TGCGCA 1 cut(s) 190
GlaI GCGC 3 cut(s) 85, 190, 256
GluI GCNGC 4 cut(s) 33, 231, 258, 261
GsuI CTGGAG 1 cut(s) 185
HaeII RGCGCY 2 cut(s) 87, 258
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 195, 209
HhaI GCGC 3 cut(s) 86, 191, 257
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 3 cut(s) 40, 61, 123
Hin6I GCGC 3 cut(s) 84, 189, 255
HinP1I GCGC 3 cut(s) 84, 189, 255
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 195, 209
HphI GGTGA 2 cut(s) 103, 195
Hpy166II GTNNAC 2 cut(s) 6, 203
Hpy188III TCNNGA 1 cut(s) 47
Hpy8I GTNNAC 2 cut(s) 6, 203
HpyAV CCTTC 2 cut(s) 53, 234
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 1 cut(s) 239
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 129, 146, 236, 245, 254
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 365
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 3 cut(s) 40, 61, 123
HspAI GCGC 3 cut(s) 84, 189, 255
KasI GGCGCC 1 cut(s) 83
Kzo9I GATC 4 cut(s) 39, 63, 78, 169
Lsp1109I GCAGC 3 cut(s) 44, 217, 247
MaeII ACGT 1 cut(s) 365
MalI GATC 4 cut(s) 41, 65, 80, 171
MboI GATC 4 cut(s) 39, 63, 78, 169
MboII GAAGA 1 cut(s) 88
MflI RGATCY 1 cut(s) 169
MhlI GDGCHC 2 cut(s) 222, 308
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 4 cut(s) 143, 150, 160, 216
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 2 cut(s) 195, 209
MspR9I CCNGG 3 cut(s) 142, 164, 337
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 3 cut(s) 142, 164, 337
MwoI GCNNNNNNNGC 6 cut(s) 17, 129, 146, 236, 245, 254
NarI GGCGCC 1 cut(s) 84
NdeII GATC 4 cut(s) 39, 63, 78, 169
NlaIII CATG 3 cut(s) 40, 61, 123
NlaIV GGNNCC 2 cut(s) 85, 161
NmeAIII GCCGAG 1 cut(s) 37
NsbI TGCGCA 1 cut(s) 190
NspI RCATGY 1 cut(s) 61
PaqCI CACCTGC 1 cut(s) 230
PasI CCCWGGG 1 cut(s) 336
PctI GAATGC 1 cut(s) 187
PinAI ACCGGT 1 cut(s) 194
PkrI GCNGC 4 cut(s) 34, 232, 259, 262
PleI GAGTC 1 cut(s) 124
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 3 cut(s) 140, 162, 335
PspGI CCWGG 3 cut(s) 140, 162, 335
PspN4I GGNNCC 2 cut(s) 85, 161
PsuI RGATCY 1 cut(s) 169
SalI GTCGAC 1 cut(s) 4
SatI GCNGC 4 cut(s) 33, 231, 258, 261
Sau3AI GATC 4 cut(s) 39, 63, 78, 169
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 3 cut(s) 142, 164, 337
SduI GDGCHC 2 cut(s) 222, 308
SetI ASST 5 cut(s) 18, 165, 208, 244, 368
SfoI GGCGCC 1 cut(s) 85
SsiI CCGC 4 cut(s) 9, 258, 308, 341
SspDI GGCGCC 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 140, 162, 335
StyI CCWWGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 368
TaqI TCGA 3 cut(s) 5, 62, 316
TauI GCSGC 1 cut(s) 260
TscAI CASTG 2 cut(s) 73, 117
TseI GCWGC 3 cut(s) 32, 230, 260
TspRI CASTG 2 cut(s) 73, 117
XceI RCATGY 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.