Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0009767

Physical Location & Seq

Genomic Coordinates Reverse (-)

36454105 .. 36459146

Gene Structure 5042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1309200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3426 bp

ATGGCGTCCAGATCGTTTAAGCCGAGCCGCTCGGATATGTCATCAAACTCTGATATGCCTGATTCCAACAACAAGACTCCAGTCCCTCCAACTGTGACATTTGGCCGCAGAACGTCCTCGGGTCGCTACATCAGCTACTCCAGGGATGATCTCGACAGTGAACTTGGGAGTGGTGAGTATATGAACTATACAGTGCACATACCACCAACCCCGGATAACCAGCCCATGGATCCATCCATCTCACAGAAGGTTGAAGAGCAATATGTGTCCAATTCCCTTTTTACGGGCGGATTTAATAGTGTTACGCGAGCTCATCTTATGGACAAGGTGATCGAATCTGAAACAAACCATCCTCAGATGGCTGGTGCTAAAGGTTCATCATGTGCAATTCCTGGCTGTGATGCAAAGGTGATGAGCGATGGACGCGGGGAGGATATTCTTCCTTGTGAGTGTGATTACAAAATATGCCGGGATTGCTATACAGATGCTGTGAAAACTGGGGGTGGTATTTGCCCTGGATGCAAGGAACCGTACAAAATCACAGATTTGGATGATGTGGGTGCGGATAACGATGCACGGCCACCGCTCCCACTTCCTCTGCCAAAAGTATTGTCTAAAAATGAGAGGAGATTGTCCCTGATGAAGTCAACAAAGTCAGTGCTAACAAGGAGTCAAACTGGGGATTTTGATCACAACCGGTGGCTCTTTGAAACAAAGGGAACTTATGGGTATGGGAATGCCATATGGCCCAAGGAGGGAGGTTTTGGAGATGATAAAGAAGATGAAGTCGTTGATAGAGCAGAGTTGTTTAACAAACCATGGAGGCCACTCACGCGGAAAATAAAAATACCTGCAGCTATTATAAGCCCGTATAGGCTTCTTATTTTTGTTCGTATGGGTGTCCTTGGGATGTTCTTGGCATGGAGGATCGATCACCCAAATACTGATGCAATGTGGCTCTGGGGAATGTCAATTGTTTGTGAGATATGGTTTGCTTTTTCTTGGCTTCTTGACCAACTGCCGAAGTTCTGTCCAATCAATCGCTCGACAGATCTTAATGTCTTGAAGGAGAAATTTGAAACACCTAGTCCCAATAACCCCTCTGGGAAATCTGATCTTCCAGGCATAGATATCTTTGTGTCCACTGCAGATCCAGACAAAGAACCACCACTTGTCACTGCAAACACTATCTTATCTATTCTAGCTACTGATTACCCTGTTGAAAAGCTAGCTTGCTATGTTTCTGATGATGGAGGTGCCCTTTTAACTTTTGAGGCCATGGCAGAAGCTGCTAGTTTTGCTAATGTATGGGCACCATTCTGCCGTAAACATGCTATTGAACCCAGGAATCCTGAATCCTACTTCAGTTTGAAGAGGGATCCATACAAGAACAAAGTGCTGCCTGACTTTGTCAAGGACCGAAGAAGGGTAAAACGTGAATATGATGAGTTCAAGGTTCGGATCAATGGACTGCCTGAATCTATACGCCGACGATCAGATGCTTATCATGCTCGGGAAGAAATCAAGGCCATGAAGCTTCAAAGAGAGAACAGGGAGGACGAACCAGTGGAGGGTGTGAAGATTCCCAAAGCAACGTGGATGGCTGATGGAACGCACTGGCCAGGGACTTGGTTGACTGCTTCACCCGAGCATTCTAAGGGTGACCATGCTGGTATTATACAGGTGATGTTGAAACCTCCCAGTGATGAACCGCTGCATGGAGCTGATGATGATGCTAGGCTCATCAACCTCACAGATGTTGATATCCGTCTTCCCATGCTTGTTTATGTGTCGCGTGAGAAACGCCCAGGCTATGATCACAACAAGAAGGCAGGGGCCATGAATGCCCTCGTTCGGGCCTCTGCCATCATGTCCAATGGCCCGTTCATTCTCAACCTTGACTGTGACCATTATATTTACAACTCTCAGGCAATGAGGGAGGGCATGTGCTTCATGATGGATCGTGGTGGTGACCGCCTTTGTTATGTCCAGTTCCCTCAGAGATTTGAGGGTATCGACCCTTCAGACCGATATGCCAATCACAACACTGTTTTCTTTGATGTCAATATGCGAGCTCTTGATGGACTTCAGGGCCCAGTTTATGTTGGAACTGGATGTCTCTTTCGTAGAGTTGCCCTTTATGGTTTTGACCCACCTCGGTCAAAAGACACTCACCCTGGTTGTTGCAGTTGCTGCTTCTCTCGTCGCAGAAAGAATACCTCTGAAGAAAACCGATCCTTAAGAATGGGTGATTCTGATGATGAAGAGATGAATCTCTCCCTGCTGCCTAAGAGGTTTGGGAACTCAACTTTCCTCATTGATTCGATTCCAGTGGCAGAGTTCCAAGGTCGTCCACTAGCAGATCACCCAGCTGTGAAGAATGGACGCCCACCTGGTGCTCTCACCATTCCCCGTGAGCTTCTTGATGCCTCTACTGTTGCAGAGGCAATCAGTGTCATTTCGTGCTGGTATGAAGACAAGACTGAGTGGGGAGAGCGCGTAGGGTGGATTTACGGATCAGTTACTGAAGATGTGGTCACTGGATATAGGATGCATAACAGAGGATGGAAATCAGTTTACTGTGTGACCAAGCGAGATGCATTCCGTGGAAGCGCTCCTATCAATCTTACAGATAGGTTGCATCAGGTTCTGCGCTGGGCTACTGGCTCAGTTGAGATTTTCTTCTCCCGCAACAACGCGCTCCTAGCCAGCTCAAGAATGAAGTTTCTGCAAAGGATAGCATACCTTAATGTGGGTATCTACCCCTTTACCTCCATCTTTCTTATCGTCTACTGCTTCCTCCCGGCGCTATCCCTCTTCTCGGGTCAGTTCATTGTGCAGAACCTCAACGTTAGTTTCCTGACTTACCTTTTGGCTATTACTCTCACTCTATGCATGCTTGCCATCCTTGAGATTAAATGGTCTGGCATCGAGCTAGAGGAGTGGTGGAGAAACGAGCAGTTTTGGTTGATTGGAGGGACTAGTGCGCACCTTGCTGCTGTGCTTCAGGGGCTACTGAAAGTAATTGCAGGGATTGAAATCTCTTTCACCTTAACTTCAAAGTCGGGTGGCGATGACGAGGATGATGAGTTTGCTGACCTCTATATTGTGAAATGGTCCTCTCTCATGATACCGCCGATAACAATCATGATGGTCAACTTAATCGGCATAGCAGTTGGGTTCAGCCGCACAATATACAGCGAAATACCAGAATGGAGCAGGTTAATTGGCGGGGTGTTCTTCAGTTTCTGGGTATTAGCGCATCTGTATCCGTTTGCCAAGGGGTTGATGGGGAGACGAGGGAAGACGCCCACCATTGTTTATGTGTGGTCAGGGCTCATTGCGATTACCATCTCTCTTCTTTGGGTGGCAATCAATCCCCCTTCGGGTGCCAGCGGAATTGGGGGATCCTTCACATTCCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000030 GO:0000139 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005794 GO:0005802 GO:0005886 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016021 GO:0016049 GO:0016740 GO:0016757 GO:0016758 GO:0019187 GO:0021700 GO:0022622 GO:0030154 GO:0030173 GO:0031090 GO:0031224 GO:0031228 GO:0031300 GO:0031301 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0051753 GO:0060560 GO:0070085 GO:0071554 GO:0071669 GO:0071695 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0097502 GO:0097708 GO:0098588 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-RING_4

Protein Analysis

1142

Amino Acids

128.05

Weight (kDa)

6.26

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_4	PF14570	130 - 179	6.4e-18	RING/Ubox like zinc-binding domain
Cellulose_synt	PF03552	376 - 1129	0.0	Cellulose synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009767)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03050 AT5G16910
fragaria_vesca	FvH4_7g34010 FvH4_7g34010 FvH4_7g34010
malus_domestica	MD01G1236500.v1.1 MD07G1309200.v1.1
prunus_persica	Prupe.2G326600_v2.0.a1 Prupe.2G326600_v2.0.a1 Prupe.2G326600_v2.0.a1 Prupe.2G326600_v2.0.a1 Prupe.2G326600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0384261
rosa_laevigata	RLG00000026053
rosa_multiflora	Rmu_sc0011271.1_g000003
rosa_rugosa	Rorug01G0446300
rosa_samantha	Rh1AG471800 Rh1BG429500 Rh1CG442100 Rh1DG462200
rosa_wichuraiana	Rw1G040080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	863
Acc16I	TGCGCA	1 cut(s)	2988
Acc36I	ACCTGC	2 cut(s)	859, 3210
AccB1I	GGYRCC	3 cut(s)	1256, 1312, 3389
AccB7I	CCANNNNNTGG	1 cut(s)	226
AccBSI	CCGCTC	2 cut(s)	30, 586
AccI	GTMKAC	1 cut(s)	2790
AccII	CGCG	6 cut(s)	307, 426, 835, 1795, 2499, 2699
AclI	AACGTT	1 cut(s)	2850
AcoI	YGGCCR	3 cut(s)	103, 578, 1619
AcsI	RAATTY	1 cut(s)	1073
AcuI	CTGAAG	7 cut(s)	1348, 2007, 2072, 2244, 2547, 2990, 3226
AcyI	GRCGYC	3 cut(s)	5, 2386, 3308
AdeI	CACNNNGTG	1 cut(s)	2396
AfaI	GTAC	1 cut(s)	533
AfeI	AGCGCT	1 cut(s)	2614
AflII	CTTAAG	1 cut(s)	2239
AgeI	ACCGGT	1 cut(s)	696
AhlI	ACTAGT	1 cut(s)	2981
AjnI	CCWGG	9 cut(s)	140, 391, 514, 1120, 1343, 1621, 1807, 2176, 2392
AjuI	GAANNNNNNNTTGG	2 cut(s)	1868, 1900
Alw21I	GWGCWC	4 cut(s)	198, 313, 2077, 2401
Alw26I	GTCTC	2 cut(s)	2123, 3289
Alw44I	GTGCAC	1 cut(s)	194
AlwNI	CAGNNNCTG	6 cut(s)	488, 1289, 2193, 2525, 2650, 3249
Ama87I	CYCGRG	4 cut(s)	118, 1512, 1646, 2821
Aor51HI	AGCGCT	1 cut(s)	2614
ApaI	GGGCCC	1 cut(s)	2096
ApaLI	GTGCAC	1 cut(s)	194
ApeKI	GCWGC	7 cut(s)	854, 1289, 1399, 1714, 2193, 2284, 2996
ApoI	RAATTY	1 cut(s)	1073
ArsI	GACNNNNNNTTYG	4 cut(s)	596, 628, 2854, 2886
AsiGI	ACCGGT	1 cut(s)	696
AspLEI	GCGC	7 cut(s)	2499, 2615, 2655, 2701, 2809, 2989, 3262
AspS9I	GGNCC	8 cut(s)	747, 1417, 1836, 1857, 1880, 2092, 2093, 3117
AsuC2I	CCSGG	3 cut(s)	212, 470, 2804
AsuNHI	GCTAGC	1 cut(s)	1228
AvaI	CYCGRG	4 cut(s)	118, 1512, 1646, 2821
AvaII	GGWCC	2 cut(s)	1417, 3117
BaeGI	GKGCMC	4 cut(s)	198, 1261, 1315, 2096
BalI	TGGCCA	1 cut(s)	1621
BamHI	GGATCC	3 cut(s)	229, 1378, 3407
BanI	GGYRCC	3 cut(s)	1256, 1312, 3389
BanII	GRGCYC	4 cut(s)	313, 2077, 2096, 3339
BarI	GAAGNNNNNNTAC	2 cut(s)	2005, 2037
BbsI	GAAGAC	3 cut(s)	1763, 2481, 3311
Bbv12I	GWGCWC	4 cut(s)	198, 313, 2077, 2401
BbvI	GCAGC	7 cut(s)	866, 1276, 1386, 1701, 2180, 2271, 2983
BceAI	ACGGC	2 cut(s)	593, 1308
BciT130I	CCWGG	9 cut(s)	142, 393, 516, 1122, 1345, 1623, 1809, 2178, 2394
BciVI	GTATCC	1 cut(s)	3279
BclI	TGATCA	2 cut(s)	688, 1816
BcnI	CCSGG	3 cut(s)	212, 470, 2804
BcoDI	GTCTC	2 cut(s)	2123, 3289
BcuI	ACTAGT	1 cut(s)	2981
BfaI	CTAG	9 cut(s)	1086, 1202, 1229, 1293, 1737, 2357, 2705, 2936, 2982
BfmI	CTRYAG	2 cut(s)	852, 1146
BfoI	RGCGCY	2 cut(s)	2616, 2810
BfrI	CTTAAG	1 cut(s)	2239
BfuAI	ACCTGC	2 cut(s)	859, 3210
BfuI	GTATCC	1 cut(s)	3279
BglII	AGATCT	1 cut(s)	1051
Bme18I	GGWCC	2 cut(s)	1417, 3117
BmeT110I	CYCGRG	4 cut(s)	118, 1512, 1646, 2821
BmgT120I	GGNCC	8 cut(s)	747, 1417, 1836, 1857, 1880, 2092, 2093, 3117
BmiI	GGNNCC	9 cut(s)	231, 528, 1258, 1314, 1380, 1837, 2094, 3391, 3409
BmrI	ACTGGG	4 cut(s)	507, 687, 1695, 2090
BmtI	GCTAGC	1 cut(s)	1232
BmuI	ACTGGG	4 cut(s)	507, 687, 1695, 2090
BoxI	GACNNNNGTC	1 cut(s)	80
BpiI	GAAGAC	3 cut(s)	1763, 2481, 3311
BpmI	CTGGAG	2 cut(s)	63, 124
BpuEI	CTTGAG	2 cut(s)	2698, 2930
BpuMI	CCSGG	3 cut(s)	212, 470, 2804
Bsa29I	ATCGAT	1 cut(s)	930
BsaBI	GATNNNNATC	6 cut(s)	687, 1503, 2569, 3038, 3143, 3350
BsaHI	GRCGYC	3 cut(s)	5, 2386, 3308
BsaWI	WCCGGW	1 cut(s)	696
BsaXI	ACNNNNNCTCC	4 cut(s)	1931, 1961, 2484, 2514
Bse118I	RCCGGY	1 cut(s)	696
Bse3DI	GCAATG	3 cut(s)	957, 1938, 3339
Bse8I	GATNNNNATC	6 cut(s)	687, 1503, 2569, 3038, 3143, 3350
BseBI	CCWGG	9 cut(s)	142, 393, 516, 1122, 1345, 1623, 1809, 2178, 2394
BseCI	ATCGAT	1 cut(s)	930
BseJI	GATNNNNATC	6 cut(s)	687, 1503, 2569, 3038, 3143, 3350
BseMI	GCAATG	3 cut(s)	957, 1938, 3339
BseMII	CTCAG	5 cut(s)	368, 1940, 2012, 2475, 2682
BseRI	GAGGAG	2 cut(s)	640, 2954
BseSI	GKGCMC	4 cut(s)	198, 1261, 1315, 2096
BseXI	GCAGC	7 cut(s)	866, 1276, 1386, 1701, 2180, 2271, 2983
BseYI	CCCAGC	2 cut(s)	2368, 2655
BsgI	GTGCAG	1 cut(s)	2858
Bsh1236I	CGCG	6 cut(s)	307, 426, 835, 1795, 2499, 2699
BshNI	GGYRCC	3 cut(s)	1256, 1312, 3389
BshTI	ACCGGT	1 cut(s)	696
BshVI	ATCGAT	1 cut(s)	930
BsiHKAI	GWGCWC	4 cut(s)	198, 313, 2077, 2401
BsiHKCI	CYCGRG	4 cut(s)	118, 1512, 1646, 2821
BsiSI	CCGG	4 cut(s)	212, 469, 697, 2804
BslFI	GGGAC	5 cut(s)	68, 619, 1074, 1639, 2992
BsmAI	GTCTC	2 cut(s)	2123, 3289
BsmBI	CGTCTC	1 cut(s)	3289
BsmFI	GGGAC	5 cut(s)	68, 619, 1074, 1639, 2992
BsmI	GAATGC	4 cut(s)	742, 1651, 1849, 2600
BsoBI	CYCGRG	4 cut(s)	118, 1512, 1646, 2821
Bsp120I	GGGCCC	1 cut(s)	2092
Bsp1286I	GDGCHC	8 cut(s)	198, 313, 1261, 1315, 2077, 2096, 2401, 3339
Bsp19I	CCATGG	3 cut(s)	225, 818, 1278
BspCNI	CTCAG	5 cut(s)	367, 1939, 2011, 2476, 2681
BspDI	ATCGAT	1 cut(s)	930
BspFNI	CGCG	6 cut(s)	307, 426, 835, 1795, 2499, 2699
BspHI	TCATGA	3 cut(s)	1953, 3126, 3147
BspLI	GGNNCC	9 cut(s)	231, 528, 1258, 1314, 1380, 1837, 2094, 3391, 3409
BspMAI	CTGCAG	2 cut(s)	856, 1150
BspMI	ACCTGC	2 cut(s)	859, 3210
BspOI	GCTAGC	1 cut(s)	1232
BspQI	GCTCTTC	1 cut(s)	249
BspT107I	GGYRCC	3 cut(s)	1256, 1312, 3389
BspTI	CTTAAG	1 cut(s)	2239
BsrBI	CCGCTC	2 cut(s)	30, 586
BsrDI	GCAATG	3 cut(s)	957, 1938, 3339
BsrFI	RCCGGY	1 cut(s)	696
BssAI	RCCGGY	1 cut(s)	696
BssNI	GRCGYC	3 cut(s)	5, 2386, 3308
BssT1I	CCWWGG	7 cut(s)	225, 750, 818, 904, 1278, 2344, 3279
Bst2UI	CCWGG	9 cut(s)	142, 393, 516, 1122, 1345, 1623, 1809, 2178, 2394
Bst4CI	ACNGT	8 cut(s)	94, 158, 193, 531, 1904, 2050, 2437, 2582
Bst6I	CTCTTC	5 cut(s)	249, 1367, 2259, 2822, 3363
BstACI	GRCGYC	3 cut(s)	5, 2386, 3308
BstAFI	CTTAAG	1 cut(s)	2239
BstAPI	GCANNNNNTGC	2 cut(s)	1289, 2193
BstC8I	GCNNGC	8 cut(s)	309, 1230, 1234, 2073, 2710, 2897, 2901, 3394
BstDEI	CTNAG	7 cut(s)	354, 1656, 1926, 1998, 2289, 2484, 2668
BstDSI	CCRYGG	4 cut(s)	225, 818, 1278, 2605
BstEII	GGTNACC	2 cut(s)	1661, 1970
BstFNI	CGCG	6 cut(s)	307, 426, 835, 1795, 2499, 2699
BstH2I	RGCGCY	2 cut(s)	2616, 2810
BstHHI	GCGC	7 cut(s)	2499, 2615, 2655, 2701, 2809, 2989, 3262
BstMAI	GTCTC	2 cut(s)	2123, 3289
BstNI	CCWGG	9 cut(s)	142, 393, 516, 1122, 1345, 1623, 1809, 2178, 2394
BstNSI	RCATGY	3 cut(s)	1334, 1948, 2899
BstPAI	GACNNNNGTC	1 cut(s)	80
BstPI	GGTNACC	2 cut(s)	1661, 1970
BstSFI	CTRYAG	2 cut(s)	852, 1146
BstSLI	GKGCMC	4 cut(s)	198, 1261, 1315, 2096
BstUI	CGCG	6 cut(s)	307, 426, 835, 1795, 2499, 2699
BstV1I	GCAGC	7 cut(s)	866, 1276, 1386, 1701, 2180, 2271, 2983
BstV2I	GAAGAC	3 cut(s)	1763, 2481, 3311
BstX2I	RGATCY	5 cut(s)	229, 1051, 1150, 1378, 3407
BstXI	CCANNNNNNTGG	1 cut(s)	1629
BstYI	RGATCY	5 cut(s)	229, 1051, 1150, 1378, 3407
Bsu15I	ATCGAT	1 cut(s)	930
BsuI	GTATCC	1 cut(s)	3279
BsuTUI	ATCGAT	1 cut(s)	930
BtgI	CCRYGG	4 cut(s)	225, 818, 1278, 2605
BtgZI	GCGATG	2 cut(s)	432, 3087
BtsI	GCAGTG	2 cut(s)	1143, 1176
BveI	ACCTGC	2 cut(s)	859, 3210
Cac8I	GCNNGC	8 cut(s)	309, 1230, 1234, 2073, 2710, 2897, 2901, 3394
CaiI	CAGNNNCTG	6 cut(s)	488, 1289, 2193, 2525, 2650, 3249
CciI	TCATGA	3 cut(s)	1953, 3126, 3147
CfoI	GCGC	7 cut(s)	2499, 2615, 2655, 2701, 2809, 2989, 3262
Cfr10I	RCCGGY	1 cut(s)	696
Cfr13I	GGNCC	8 cut(s)	747, 1417, 1836, 1857, 1880, 2092, 2093, 3117
ClaI	ATCGAT	1 cut(s)	930
CseI	GACGC	3 cut(s)	432, 2394, 3316
CsiI	ACCWGGT	1 cut(s)	2392
Csp6I	GTAC	1 cut(s)	532
CspAI	ACCGGT	1 cut(s)	696
CviQI	GTAC	1 cut(s)	532
DdeI	CTNAG	7 cut(s)	354, 1656, 1926, 1998, 2289, 2484, 2668
DraIII	CACNNNGTG	1 cut(s)	2396
EaeI	YGGCCR	3 cut(s)	103, 578, 1619
Eam1104I	CTCTTC	5 cut(s)	249, 1367, 2259, 2822, 3363
EarI	CTCTTC	5 cut(s)	249, 1367, 2259, 2822, 3363
EciI	GGCGGA	1 cut(s)	303
Ecl136II	GAGCTC	2 cut(s)	311, 2075
Eco130I	CCWWGG	7 cut(s)	225, 750, 818, 904, 1278, 2344, 3279
Eco24I	GRGCYC	4 cut(s)	313, 2077, 2096, 3339
Eco32I	GATATC	2 cut(s)	1132, 1765
Eco47I	GGWCC	2 cut(s)	1417, 3117
Eco47III	AGCGCT	1 cut(s)	2614
Eco53kI	GAGCTC	2 cut(s)	311, 2075
Eco57I	CTGAAG	7 cut(s)	1348, 2007, 2072, 2244, 2547, 2990, 3226
Eco88I	CYCGRG	4 cut(s)	118, 1512, 1646, 2821
Eco91I	GGTNACC	2 cut(s)	1661, 1970
EcoICRI	GAGCTC	2 cut(s)	311, 2075
EcoO109I	RGGNCCY	1 cut(s)	2092
EcoO65I	GGTNACC	2 cut(s)	1661, 1970
EcoRII	CCWGG	9 cut(s)	140, 391, 514, 1120, 1343, 1621, 1807, 2176, 2392
EcoRV	GATATC	2 cut(s)	1132, 1765
EcoT14I	CCWWGG	7 cut(s)	225, 750, 818, 904, 1278, 2344, 3279
EcoT22I	ATGCAT	3 cut(s)	2556, 2602, 2897
EcoT38I	GRGCYC	4 cut(s)	313, 2077, 2096, 3339
ErhI	CCWWGG	7 cut(s)	225, 750, 818, 904, 1278, 2344, 3279
Esp3I	CGTCTC	1 cut(s)	3289
FaqI	GGGAC	5 cut(s)	68, 619, 1074, 1639, 2992
FauI	CCCGC	3 cut(s)	419, 2696, 3224
FauNDI	CATATG	1 cut(s)	743
FbaI	TGATCA	2 cut(s)	688, 1816
FblI	GTMKAC	1 cut(s)	2790
FriOI	GRGCYC	4 cut(s)	313, 2077, 2096, 3339
FspAI	RTGCGCAY	1 cut(s)	2988
FspBI	CTAG	9 cut(s)	1086, 1202, 1229, 1293, 1737, 2357, 2705, 2936, 2982
FspI	TGCGCA	1 cut(s)	2988
GlaI	GCGC	7 cut(s)	2498, 2614, 2654, 2700, 2808, 2988, 3261
GsaI	CCCAGC	2 cut(s)	2372, 2659
GsuI	CTGGAG	2 cut(s)	63, 124
HaeII	RGCGCY	2 cut(s)	2616, 2810
HapII	CCGG	4 cut(s)	212, 469, 697, 2804
HgaI	GACGC	3 cut(s)	432, 2394, 3316
HhaI	GCGC	7 cut(s)	2499, 2615, 2655, 2701, 2809, 2989, 3262
Hin1I	GRCGYC	3 cut(s)	5, 2386, 3308
Hin6I	GCGC	7 cut(s)	2497, 2613, 2653, 2699, 2807, 2987, 3260
HinP1I	GCGC	7 cut(s)	2497, 2613, 2653, 2699, 2807, 2987, 3260
HincII	GTYRAC	3 cut(s)	648, 1635, 3157
HindII	GTYRAC	3 cut(s)	648, 1635, 3157
HindIII	AAGCTT	1 cut(s)	1535
HpaII	CCGG	4 cut(s)	212, 469, 697, 2804
Hpy99I	CGWCG	2 cut(s)	1494, 2208
HpyAV	CCTTC	7 cut(s)	241, 1060, 1419, 1822, 2031, 3393, 3421
HpyCH4III	ACNGT	8 cut(s)	94, 158, 193, 531, 1904, 2050, 2437, 2582
HpyCH4IV	ACGT	4 cut(s)	113, 1435, 1595, 2850
HpyF3I	CTNAG	7 cut(s)	354, 1656, 1926, 1998, 2289, 2484, 2668
HpySE526I	ACGT	4 cut(s)	113, 1435, 1595, 2850
Hsp92I	GRCGYC	3 cut(s)	5, 2386, 3308
HspAI	GCGC	7 cut(s)	2497, 2613, 2653, 2699, 2807, 2987, 3260
Ksp22I	TGATCA	2 cut(s)	688, 1816
LguI	GCTCTTC	1 cut(s)	249
LmnI	GCTCC	5 cut(s)	591, 1721, 2620, 2706, 3216
Lsp1109I	GCAGC	7 cut(s)	866, 1276, 1386, 1701, 2180, 2271, 2983
MabI	ACCWGGT	1 cut(s)	2392
MaeI	CTAG	9 cut(s)	1086, 1202, 1229, 1293, 1737, 2357, 2705, 2936, 2982
MaeII	ACGT	4 cut(s)	113, 1435, 1595, 2850
MaeIII	GTNAC	9 cut(s)	94, 301, 1174, 1661, 1904, 1970, 2521, 2536, 2584
MbiI	CCGCTC	2 cut(s)	30, 586
MfeI	CAATTG	1 cut(s)	972
MflI	RGATCY	5 cut(s)	229, 1051, 1150, 1378, 3407
MhlI	GDGCHC	8 cut(s)	198, 313, 1261, 1315, 2077, 2096, 2401, 3339
MlsI	TGGCCA	1 cut(s)	1621
MluCI	AATT	7 cut(s)	271, 387, 972, 1073, 3024, 3225, 3399
MluNI	TGGCCA	1 cut(s)	1621
MlyI	GAGTC	2 cut(s)	70, 679
MmeI	TCCRAC	3 cut(s)	90, 113, 2086
Mox20I	TGGCCA	1 cut(s)	1621
Mph1103I	ATGCAT	3 cut(s)	2556, 2602, 2897
MscI	TGGCCA	1 cut(s)	1621
Msp20I	TGGCCA	1 cut(s)	1621
MspA1I	CMGCKG	3 cut(s)	1714, 2372, 3396
MspCI	CTTAAG	1 cut(s)	2239
MspI	CCGG	4 cut(s)	212, 469, 697, 2804
MunI	CAATTG	1 cut(s)	972
Mva1269I	GAATGC	4 cut(s)	742, 1651, 1849, 2600
MvaI	CCWGG	9 cut(s)	142, 393, 516, 1122, 1345, 1623, 1809, 2178, 2394
MvnI	CGCG	6 cut(s)	307, 426, 835, 1795, 2499, 2699
NciI	CCSGG	3 cut(s)	212, 470, 2804
NcoI	CCATGG	3 cut(s)	225, 818, 1278
NdeI	CATATG	1 cut(s)	743
NheI	GCTAGC	1 cut(s)	1228
NlaIV	GGNNCC	9 cut(s)	231, 528, 1258, 1314, 1380, 1837, 2094, 3391, 3409
NmeAIII	GCCGAG	1 cut(s)	48
NmuCI	GTSAC	7 cut(s)	94, 1174, 1661, 1904, 1970, 2536, 2584
NsbI	TGCGCA	1 cut(s)	2988
NsiI	ATGCAT	3 cut(s)	2556, 2602, 2897
NspI	RCATGY	3 cut(s)	1334, 1948, 2899
PaeI	GCATGC	1 cut(s)	2899
PagI	TCATGA	3 cut(s)	1953, 3126, 3147
PciSI	GCTCTTC	1 cut(s)	249
PcsI	WCGNNNNNNNCGW	1 cut(s)	20
PctI	GAATGC	4 cut(s)	742, 1651, 1849, 2600
PflFI	GACNNNGTC	1 cut(s)	1409
PflMI	CCANNNNNTGG	1 cut(s)	226
PinAI	ACCGGT	1 cut(s)	696
PleI	GAGTC	2 cut(s)	70, 678
PpsI	GAGTC	2 cut(s)	70, 678
PshAI	GACNNNNGTC	1 cut(s)	80
PsiI	TTATAA	1 cut(s)	863
Psp124BI	GAGCTC	2 cut(s)	313, 2077
Psp1406I	AACGTT	1 cut(s)	2850
Psp6I	CCWGG	9 cut(s)	140, 391, 514, 1120, 1343, 1621, 1807, 2176, 2392
PspEI	GGTNACC	2 cut(s)	1661, 1970
PspFI	CCCAGC	2 cut(s)	2368, 2655
PspGI	CCWGG	9 cut(s)	140, 391, 514, 1120, 1343, 1621, 1807, 2176, 2392
PspN4I	GGNNCC	9 cut(s)	231, 528, 1258, 1314, 1380, 1837, 2094, 3391, 3409
PspOMI	GGGCCC	1 cut(s)	2092
PspPI	GGNCC	8 cut(s)	747, 1417, 1836, 1857, 1880, 2092, 2093, 3117
PstI	CTGCAG	2 cut(s)	856, 1150
PstNI	CAGNNNCTG	6 cut(s)	488, 1289, 2193, 2525, 2650, 3249
PsuI	RGATCY	5 cut(s)	229, 1051, 1150, 1378, 3407
PsyI	GACNNNGTC	1 cut(s)	1409
PvuII	CAGCTG	1 cut(s)	2372
RsaI	GTAC	1 cut(s)	533
RsaNI	GTAC	1 cut(s)	532
SacI	GAGCTC	2 cut(s)	313, 2077
SapI	GCTCTTC	1 cut(s)	249
Sau96I	GGNCC	8 cut(s)	747, 1417, 1836, 1857, 1880, 2092, 2093, 3117
SchI	GAGTC	2 cut(s)	70, 679
SduI	GDGCHC	8 cut(s)	198, 313, 1261, 1315, 2077, 2096, 2401, 3339
SexAI	ACCWGGT	1 cut(s)	2392
SfcI	CTRYAG	2 cut(s)	852, 1146
SinI	GGWCC	2 cut(s)	1417, 3117
SmlI	CTYRAG	3 cut(s)	2239, 2713, 2909
SmoI	CTYRAG	3 cut(s)	2239, 2713, 2909
SpeI	ACTAGT	1 cut(s)	2981
SphI	GCATGC	1 cut(s)	2899
Sse9I	AATT	7 cut(s)	271, 387, 972, 1073, 3024, 3225, 3399
SspMI	CTAG	9 cut(s)	1086, 1202, 1229, 1293, 1737, 2357, 2705, 2936, 2982
SstI	GAGCTC	2 cut(s)	313, 2077
StyI	CCWWGG	7 cut(s)	225, 750, 818, 904, 1278, 2344, 3279
TaaI	ACNGT	8 cut(s)	94, 158, 193, 531, 1904, 2050, 2437, 2582
TaiI	ACGT	4 cut(s)	116, 1438, 1598, 2853
TaqI	TCGA	7 cut(s)	153, 333, 930, 1046, 2016, 2324, 2931
TaqII	GACCGA	3 cut(s)	1434, 2043, 2148
TasI	AATT	7 cut(s)	271, 387, 972, 1073, 3024, 3225, 3399
TauI	GCSGC	3 cut(s)	30, 108, 3189
TseFI	GTSAC	7 cut(s)	94, 1174, 1661, 1904, 1970, 2536, 2584
TseI	GCWGC	7 cut(s)	854, 1289, 1399, 1714, 2193, 2284, 2996
Tsp45I	GTSAC	7 cut(s)	94, 1174, 1661, 1904, 1970, 2536, 2584
TspGWI	ACGGA	4 cut(s)	1757, 2529, 2594, 3261
Tth111I	GACNNNGTC	1 cut(s)	1409
Van91I	CCANNNNNTGG	1 cut(s)	226
Vha464I	CTTAAG	1 cut(s)	2239
VneI	GTGCAC	1 cut(s)	194
VpaK11BI	GGWCC	2 cut(s)	1417, 3117
XapI	RAATTY	1 cut(s)	1073
XceI	RCATGY	3 cut(s)	1334, 1948, 2899
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3286
XmiI	GTMKAC	1 cut(s)	2790
XspI	CTAG	9 cut(s)	1086, 1202, 1229, 1293, 1737, 2357, 2705, 2936, 2982
Zsp2I	ATGCAT	3 cut(s)	2556, 2602, 2897

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.