MD08G1003700.v1.1

ATP-dependent zinc metalloprotease FTSH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011868
Physical Location & Seq
Reverse (-)
372855 .. 375798
2944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1003700.v1.1.491

Sequence Viewer

Length: 2157 bp
ATGGCTTCCACCACCAACCCTTTGCTCTTTTCCAACTTCTTCGGAGCCACCACAGTCCTCCACTCTCCGCCCACACCTAAAACCACCTCCAAATCCAACTCATCCCTCCCAATTCCCCTGCCCTTCTTCTCCAAGCGAAAGCTTTTCGCCACCAAAAACATCTTCAACTCTCAACCCGATTCGGAGCCGTTAAAATCCGCAGCTTCCCAAGCCACTTTAGCCGCCCTGCTCTTCTCCTCACTCACCCCACAAGCCCTAGCCATAGACAACACCCCCACTCCCACGCCGCCACCCGTACTCCAACCCCAGCCTTCCAAATCCAACGCCGCCTCTCCTTTCTCCCAAAACCTCCTCCTCAACGCCCCTAAGCCCCAATCCCAGGTCGCCACTGACCTCCCCGAAGGCAGCCAATGGCGCTACAGCGAGTTCCTCAACGCCGTCAAGAAGGGCAAAGTCGAGAGAGTCAGGTTCATGAAAGACGGTTCTGCCCTTCAGCTCACTGCCGTCGATGGCCGCCGCGCCTCTGTCGTTGTCCCCAACGACCCTGACCTCATCGACATTTTGGCCATGAACGGCGTCGATATTTCCGTCTCCGAAGGCGATTCGGGAGGTGGGCTGTTCAATATCATCGGCAATTTGTTGTTCCCAATCTTGGCCTTTGCCGGATTGTTCCTCCTCTTCCGGCGTGCTGGAGGAGGCCCAGGAGGGCCGGGCGGCCTGGGCGGGCCCATGGATTTCGGAAGGTCCAAGTCCAAATTCCAAGAAGTTCCGGAAACGGGGGTGACTTTCGCCGATGTAGCTGGAGCTGACCAAGCAAAATTGGAGCTGCAGGAGGTGGTGGATTTCTTGAAGAACCCAGACAAGTACACCGCCCTCGGCGCCAAAATCCCAAAAGGATGTCTTTTGGTGGGCCCACCTGGTACCGGGAAGACCCTTCTGGCTCGCGCTGTGGCCGGCGAGGCTGGGACGCCGTTTTTCTCGTGCGCGGCTTCCGAGTTCGTGGAGTTGTTTGTGGGAGTGGGGGCGTCGAGAGTACGGGACTTGTTCGAGAAGGCCAAGTCGAAAGCGCCGTGCATTGTGTTCATTGATGAGATAGATGCGGTGGGGAGGCAGAGAGGCGCGGGAATGGGCGGCGGGAATGATGAGAGGGAGCAAACTATTAATCAGCTGCTGACGGAGATGGATGGGTTTTCTGGTAATTCCGGTGTGATAGTTTTGGCGGCAACCAACAGGCCGGATGTTCTCGATTCGGCATTGTTGAGGCCCGGGAGGTTTGACCGACAGGTGACCGTCGACAGGCCTGATGTTGCTGGCAGAGTTAAAATCCTGCAGGTGCATTCCAGAGGGAAGGCGCTTGCAAAGGATGTGGACTTTGACAAAATTGCAAGGAGAACACCTGGTTTCACAGGGGCTGATCTGCAGAACCTGATGAATGAGGCTGCCATTCTCGCAGCCAGACGTGATCTCAAAGAAATAAGTAAAGACGAGATCTCCGATGCTCTGGAGCGGATCATTGCAGGACCCGAGAAGAAAAATGCTGTTGTCTCAGAGGATAAGAAGAAATTAGTCGCCTACCATGAGGCGGGGCATGCTCTTGTTGGTGCATTGATGCCGGAATACGATCCTGTTGCTAAGATATCCATCATTCCTCGTGGCCAAGCCGGCGGTCTAACATTCTTTGCTCCCAGTGAAGAGAGACTCGAGTCTGGACTATACAGTAGAAGCTACCTGGAGAATCAAATGGCTGTTGCCCTCGGTGGAAGAGTTGCCGAAGAAGTTATCTTTGGTCAGGAGAATGTGACAACAGGGGCTTCTAGTGATTTCATGCAAGTGTCAAGGGTGGCAAGGCAGATGGTTGAGAGATTTGGGTTCAGCAAAAAGATTGGACAAGTCGCGATCGGTGCTTCTGGTGGCAATCCCTTCTTAGGGCAACAGATGTCGTCCCAAAAAGACTACTCCATGGCAACTGCTGATGTGGTGGATGCAGAGGTAAGGGAATTGGTAGAGACAGCTTATTCAAGGGCCAAGGATATCGTCACAACTCACATCGATATCCTCCACACGCTTGCCCAGCTCCTCATGGAGAAGGAAACTGTTGACGGTGAAGAGTTCATGAGCCTCTTTATTGATGGAAAGGCTGAGCTATATGTCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA Peptidase_M41

Protein Analysis

719

Amino Acids

76.65

Weight (kDa)

5.8

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 300 - 432 2.9e-45 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 455 - 498 1.1e-16 AAA+ lid domain
Peptidase_M41 PF01434 514 - 706 3.2e-71 Peptidase family M41
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011868)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50250 AT5G42270
fragaria_vesca FvH4_2g37610 FvH4_2g37610 FvH4_2g37610 FvH4_2g37610
malus_domestica MD08G1003700.v1.1 MD15G1003200.v1.1
prunus_persica Prupe.1G355600_v2.0.a1 Prupe.1G355600_v2.0.a1
pyrus_communis pycom15g00230
rosa_chinensis RchiOBHm_Chr6g0304121
rosa_laevigata RLG00000011013
rosa_multiflora Rmu_ssc0000218.1_g000001 Rmu_ssc0000218.1_g000004
rosa_roxburghii Rroxscaffold_7G00164050
rosa_rugosa Rorug06G0327800
rosa_samantha Rh6BG461000
rosa_wichuraiana Rw6G038290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1321
Acc36I ACCTGC 1 cut(s) 1321
Acc65I GGTACC 1 cut(s) 920
AccB1I GGYRCC 2 cut(s) 878, 920
AccBSI CCGCTC 1 cut(s) 1507
AccI GTMKAC 1 cut(s) 1293
AccII CGCG 5 cut(s) 519, 945, 986, 1121, 1895
AccIII TCCGGA 1 cut(s) 769
AclWI GGATC 2 cut(s) 1517, 1616
AcoI YGGCCR 4 cut(s) 511, 564, 951, 1654
AcsI RAATTY 1 cut(s) 755
AcuI CTGAAG 1 cut(s) 476
AcyI GRCGYC 4 cut(s) 576, 879, 968, 1025
AfaI GTAC 4 cut(s) 297, 866, 922, 1035
AfiI CCNNNNNNNGG 8 cut(s) 379, 652, 776, 923, 1296, 1582, 1925, 1926
AgsI TTSAA 4 cut(s) 166, 622, 850, 2019
AjiI CACGTC 1 cut(s) 1460
AjnI CCWGG 6 cut(s) 378, 700, 717, 916, 1396, 1728
AjuI GAANNNNNNNTTGG 6 cut(s) 146, 178, 740, 772, 1767, 1799
Alw26I GTCTC 4 cut(s) 595, 1549, 1690, 2000
AlwI GGATC 2 cut(s) 1517, 1616
AlwNI CAGNNNCTG 3 cut(s) 1171, 1412, 1426
Ama87I CYCGRG 3 cut(s) 1265, 1523, 1700
Aor13HI TCCGGA 1 cut(s) 769
ApaI GGGCCC 2 cut(s) 729, 914
ApeKI GCWGC 6 cut(s) 200, 405, 826, 1168, 1439, 1451
ApoI RAATTY 1 cut(s) 755
AseI ATTAAT 1 cut(s) 1161
Asp718I GGTACC 1 cut(s) 920
AspLEI GCGC 8 cut(s) 417, 521, 881, 947, 986, 1069, 1121, 1354
AsuC2I CCSGG 4 cut(s) 711, 925, 1266, 1267
AsuHPI GGTGA 4 cut(s) 235, 793, 1297, 2114
AvaI CYCGRG 3 cut(s) 1265, 1523, 1700
AvaII GGWCC 2 cut(s) 744, 1520
BaeGI GKGCMC 2 cut(s) 729, 914
BalI TGGCCA 2 cut(s) 566, 1656
BanI GGYRCC 2 cut(s) 878, 920
BanII GRGCYC 2 cut(s) 729, 914
BauI CACGAG 2 cut(s) 979, 1650
BbsI GAAGAC 1 cut(s) 935
BbvI GCAGC 6 cut(s) 212, 417, 813, 1155, 1426, 1463
BccI CCATC 6 cut(s) 503, 1174, 1178, 1649, 1846, 2123
BceAI ACGGC 6 cut(s) 172, 422, 488, 589, 955, 1054
BcgI CGANNNNNNTGC 2 cut(s) 1610, 1644
BciT130I CCWGG 6 cut(s) 380, 702, 719, 918, 1398, 1730
BcnI CCSGG 4 cut(s) 711, 925, 1266, 1267
BcoDI GTCTC 4 cut(s) 595, 1549, 1690, 2000
BfaI CTAG 2 cut(s) 257, 1815
BfmI CTRYAG 4 cut(s) 418, 827, 1328, 1418
BfoI RGCGCY 4 cut(s) 418, 882, 1070, 1355
BfuAI ACCTGC 1 cut(s) 1321
BglI GCCNNNNNGGC 2 cut(s) 959, 1662
BglII AGATCT 1 cut(s) 1488
BlpI GCTNAGC 1 cut(s) 2139
Bme18I GGWCC 2 cut(s) 744, 1520
BmeT110I CYCGRG 3 cut(s) 1265, 1523, 1700
BmgBI CACGTC 1 cut(s) 1460
BmiI GGNNCC 7 cut(s) 46, 186, 727, 880, 912, 922, 1522
BmrI ACTGGG 1 cut(s) 1680
BmsI GCATC 4 cut(s) 1087, 1486, 1599, 1972
BmuI ACTGGG 1 cut(s) 1680
BoxI GACNNNNGTC 1 cut(s) 1702
BpiI GAAGAC 1 cut(s) 935
BpmI CTGGAG 4 cut(s) 711, 822, 1523, 1751
Bpu10I CCTNAGC 1 cut(s) 366
Bpu1102I GCTNAGC 1 cut(s) 2139
BpuMI CCSGG 4 cut(s) 711, 925, 1266, 1267
Bsa29I ATCGAT 1 cut(s) 2049
BsaBI GATNNNNATC 1 cut(s) 1640
BsaHI GRCGYC 4 cut(s) 576, 879, 968, 1025
BsaJI CCNNGG 9 cut(s) 378, 700, 718, 729, 874, 1265, 1753, 1959, 2025
BsaWI WCCGGW 2 cut(s) 769, 1202
BsaXI ACNNNNNCTCC 6 cut(s) 262, 282, 292, 312, 1475, 1505
Bsc4I CCNNNNNNNGG 8 cut(s) 379, 652, 776, 923, 1296, 1582, 1925, 1926
Bse118I RCCGGY 2 cut(s) 953, 1661
Bse1I ACTGG 1 cut(s) 1686
Bse3DI GCAATG 1 cut(s) 1512
Bse8I GATNNNNATC 1 cut(s) 1640
BseAI TCCGGA 1 cut(s) 769
BseBI CCWGG 6 cut(s) 380, 702, 719, 918, 1398, 1730
BseCI ATCGAT 1 cut(s) 2049
BseDI CCNNGG 9 cut(s) 378, 700, 718, 729, 874, 1265, 1753, 1959, 2025
BseGI GGATG 6 cut(s) 101, 902, 1189, 1243, 1369, 1987
BseJI GATNNNNATC 1 cut(s) 1640
BseLI CCNNNNNNNGG 8 cut(s) 379, 652, 776, 923, 1296, 1582, 1925, 1926
BseMI GCAATG 1 cut(s) 1512
BseMII CTCAG 2 cut(s) 1560, 2130
BseNI ACTGG 1 cut(s) 1686
BseRI GAGGAG 6 cut(s) 226, 341, 344, 665, 708, 2066
BseSI GKGCMC 2 cut(s) 729, 914
BseXI GCAGC 6 cut(s) 212, 417, 813, 1155, 1426, 1463
BseYI CCCAGC 3 cut(s) 306, 962, 2070
Bsh1236I CGCG 5 cut(s) 519, 945, 986, 1121, 1895
Bsh1285I CGRYCG 1 cut(s) 1899
BshNI GGYRCC 2 cut(s) 878, 920
BshVI ATCGAT 1 cut(s) 2049
BsiEI CGRYCG 1 cut(s) 1899
BsiHKCI CYCGRG 3 cut(s) 1265, 1523, 1700
BslFI GGGAC 4 cut(s) 518, 979, 1052, 1927
BslI CCNNNNNNNGG 8 cut(s) 379, 652, 776, 923, 1296, 1582, 1925, 1926
BsmAI GTCTC 4 cut(s) 595, 1549, 1690, 2000
BsmBI CGTCTC 1 cut(s) 595
BsmFI GGGAC 4 cut(s) 518, 979, 1052, 1927
BsmI GAATGC 1 cut(s) 1336
BsoBI CYCGRG 3 cut(s) 1265, 1523, 1700
Bsp120I GGGCCC 2 cut(s) 725, 910
Bsp1286I GDGCHC 2 cut(s) 729, 914
Bsp13I TCCGGA 1 cut(s) 769
Bsp143I GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
Bsp1720I GCTNAGC 1 cut(s) 2139
Bsp19I CCATGG 2 cut(s) 729, 1959
Bsp68I TCGCGA 1 cut(s) 1895
BspCNI CTCAG 2 cut(s) 1559, 2131
BspDI ATCGAT 1 cut(s) 2049
BspEI TCCGGA 1 cut(s) 769
BspFNI CGCG 5 cut(s) 519, 945, 986, 1121, 1895
BspHI TCATGA 2 cut(s) 471, 2112
BspLI GGNNCC 7 cut(s) 46, 186, 727, 880, 912, 922, 1522
BspMAI CTGCAG 3 cut(s) 831, 1332, 1422
BspMI ACCTGC 1 cut(s) 1321
BspPI GGATC 2 cut(s) 1517, 1616
BspQI GCTCTTC 1 cut(s) 236
BspT107I GGYRCC 2 cut(s) 878, 920
BsrBI CCGCTC 1 cut(s) 1507
BsrDI GCAATG 1 cut(s) 1512
BsrFI RCCGGY 2 cut(s) 953, 1661
BsrI ACTGG 1 cut(s) 1686
BssAI RCCGGY 2 cut(s) 953, 1661
BssECI CCNNGG 9 cut(s) 378, 700, 718, 729, 874, 1265, 1753, 1959, 2025
BssMI GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
BssNI GRCGYC 4 cut(s) 576, 879, 968, 1025
BssSI CACGAG 2 cut(s) 979, 1650
BssT1I CCWWGG 3 cut(s) 729, 1959, 2025
Bst2BI CACGAG 2 cut(s) 979, 1650
Bst2UI CCWGG 6 cut(s) 380, 702, 719, 918, 1398, 1730
Bst4CI ACNGT 6 cut(s) 55, 482, 1291, 1718, 2095, 2102
Bst6I CTCTTC 5 cut(s) 236, 683, 1686, 1756, 2100
BstACI GRCGYC 4 cut(s) 576, 879, 968, 1025
BstC8I GCNNGC 9 cut(s) 687, 725, 943, 955, 1312, 1356, 1590, 1663, 2067
BstDEI CTNAG 6 cut(s) 366, 1546, 1632, 1924, 2139, 2154
BstDSI CCRYGG 2 cut(s) 729, 1959
BstEII GGTNACC 1 cut(s) 1285
BstF5I GGATG 6 cut(s) 101, 902, 1189, 1243, 1369, 1987
BstFNI CGCG 5 cut(s) 519, 945, 986, 1121, 1895
BstH2I RGCGCY 4 cut(s) 418, 882, 1070, 1355
BstHHI GCGC 8 cut(s) 417, 521, 881, 947, 986, 1069, 1121, 1354
BstKTI GATC 6 cut(s) 1417, 1465, 1491, 1512, 1624, 1899
BstMAI GTCTC 4 cut(s) 595, 1549, 1690, 2000
BstMBI GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
BstMCI CGRYCG 1 cut(s) 1899
BstNI CCWGG 6 cut(s) 380, 702, 719, 918, 1398, 1730
BstNSI RCATGY 1 cut(s) 1592
BstPAI GACNNNNGTC 1 cut(s) 1702
BstPI GGTNACC 1 cut(s) 1285
BstSFI CTRYAG 4 cut(s) 418, 827, 1328, 1418
BstSLI GKGCMC 2 cut(s) 729, 914
BstUI CGCG 5 cut(s) 519, 945, 986, 1121, 1895
BstV1I GCAGC 6 cut(s) 212, 417, 813, 1155, 1426, 1463
BstV2I GAAGAC 1 cut(s) 935
BstX2I RGATCY 1 cut(s) 1488
BstYI RGATCY 1 cut(s) 1488
Bsu15I ATCGAT 1 cut(s) 2049
BsuTUI ATCGAT 1 cut(s) 2049
BtgI CCRYGG 2 cut(s) 729, 1959
BtrI CACGTC 1 cut(s) 1460
BtsCI GGATG 6 cut(s) 101, 902, 1189, 1243, 1369, 1987
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 3 cut(s) 387, 498, 1693
BtuMI TCGCGA 1 cut(s) 1895
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 9 cut(s) 687, 725, 943, 955, 1312, 1356, 1590, 1663, 2067
CaiI CAGNNNCTG 3 cut(s) 1171, 1412, 1426
CciI TCATGA 2 cut(s) 471, 2112
CfoI GCGC 8 cut(s) 417, 521, 881, 947, 986, 1069, 1121, 1354
Cfr10I RCCGGY 2 cut(s) 953, 1661
Cfr9I CCCGGG 1 cut(s) 1265
ClaI ATCGAT 1 cut(s) 2049
CseI GACGC 3 cut(s) 565, 976, 1014
CsiI ACCWGGT 2 cut(s) 916, 1396
Csp6I GTAC 4 cut(s) 296, 865, 921, 1034
CspCI CAANNNNNGTGG 2 cut(s) 1347, 1382
CviAII CATG 9 cut(s) 472, 568, 730, 1577, 1589, 1825, 1960, 2080, 2113
CviQI GTAC 4 cut(s) 296, 865, 921, 1034
DdeI CTNAG 6 cut(s) 366, 1546, 1632, 1924, 2139, 2154
DinI GGCGCC 1 cut(s) 880
DpnI GATC 6 cut(s) 1416, 1464, 1490, 1511, 1623, 1898
DpnII GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
EaeI YGGCCR 4 cut(s) 511, 564, 951, 1654
Eam1104I CTCTTC 5 cut(s) 236, 683, 1686, 1756, 2100
EarI CTCTTC 5 cut(s) 236, 683, 1686, 1756, 2100
EciI GGCGGA 1 cut(s) 57
Eco130I CCWWGG 3 cut(s) 729, 1959, 2025
Eco147I AGGCCT 1 cut(s) 1300
Eco24I GRGCYC 2 cut(s) 729, 914
Eco32I GATATC 3 cut(s) 1638, 2032, 2053
Eco47I GGWCC 2 cut(s) 744, 1520
Eco57I CTGAAG 1 cut(s) 476
Eco88I CYCGRG 3 cut(s) 1265, 1523, 1700
Eco91I GGTNACC 1 cut(s) 1285
EcoO109I RGGNCCY 1 cut(s) 1520
EcoO65I GGTNACC 1 cut(s) 1285
EcoRII CCWGG 6 cut(s) 378, 700, 717, 916, 1396, 1728
EcoRV GATATC 3 cut(s) 1638, 2032, 2053
EcoT14I CCWWGG 3 cut(s) 729, 1959, 2025
EcoT38I GRGCYC 2 cut(s) 729, 914
EgeI GGCGCC 1 cut(s) 880
EheI GGCGCC 1 cut(s) 880
ErhI CCWWGG 3 cut(s) 729, 1959, 2025
Esp3I CGTCTC 1 cut(s) 595
FaeI CATG 9 cut(s) 475, 571, 733, 1580, 1592, 1828, 1963, 2083, 2116
FalI AAGNNNNNCTT 2 cut(s) 885, 917
FaqI GGGAC 4 cut(s) 518, 979, 1052, 1927
FatI CATG 9 cut(s) 471, 567, 729, 1576, 1588, 1824, 1959, 2079, 2112
FauI CCCGC 4 cut(s) 716, 1114, 1127, 1576
FblI GTMKAC 1 cut(s) 1293
FokI GGATG 6 cut(s) 88, 909, 1196, 1250, 1376, 1994
FriOI GRGCYC 2 cut(s) 729, 914
FspBI CTAG 2 cut(s) 257, 1815
GlaI GCGC 8 cut(s) 416, 520, 880, 946, 985, 1068, 1120, 1353
GsaI CCCAGC 3 cut(s) 310, 966, 2074
GsuI CTGGAG 4 cut(s) 711, 822, 1523, 1751
HaeII RGCGCY 4 cut(s) 418, 882, 1070, 1355
HgaI GACGC 3 cut(s) 565, 976, 1014
HhaI GCGC 8 cut(s) 417, 521, 881, 947, 986, 1069, 1121, 1354
Hin1I GRCGYC 4 cut(s) 576, 879, 968, 1025
Hin1II CATG 9 cut(s) 475, 571, 733, 1580, 1592, 1828, 1963, 2083, 2116
Hin6I GCGC 8 cut(s) 415, 519, 879, 945, 984, 1067, 1119, 1352
HinP1I GCGC 8 cut(s) 415, 519, 879, 945, 984, 1067, 1119, 1352
HincII GTYRAC 2 cut(s) 1294, 2098
HindII GTYRAC 2 cut(s) 1294, 2098
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 7 cut(s) 179, 462, 602, 1247, 1698, 1703, 1735
HphI GGTGA 4 cut(s) 235, 793, 1297, 2114
Hpy166II GTNNAC 4 cut(s) 867, 1294, 1369, 2098
Hpy188I TCNGA 7 cut(s) 44, 184, 595, 740, 994, 1495, 1549
Hpy8I GTNNAC 4 cut(s) 867, 1294, 1369, 2098
Hpy99I CGWCG 4 cut(s) 509, 581, 1030, 1295
HpyCH4III ACNGT 6 cut(s) 55, 482, 1291, 1718, 2095, 2102
HpyCH4IV ACGT 1 cut(s) 1459
HpyF3I CTNAG 6 cut(s) 366, 1546, 1632, 1924, 2139, 2154
HpySE526I ACGT 1 cut(s) 1459
Hsp92I GRCGYC 4 cut(s) 576, 879, 968, 1025
Hsp92II CATG 9 cut(s) 475, 571, 733, 1580, 1592, 1828, 1963, 2083, 2116
HspAI GCGC 8 cut(s) 415, 519, 879, 945, 984, 1067, 1119, 1352
KasI GGCGCC 1 cut(s) 878
Kpn2I TCCGGA 1 cut(s) 769
KpnI GGTACC 1 cut(s) 924
KroI GCCGGC 2 cut(s) 953, 1661
KroNI GCCGGC 2 cut(s) 955, 1663
Kzo9I GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
LguI GCTCTTC 1 cut(s) 236
LmnI GCTCC 8 cut(s) 44, 184, 803, 823, 1150, 1504, 1687, 2079
Lsp1109I GCAGC 6 cut(s) 212, 417, 813, 1155, 1426, 1463
LweI GCATC 4 cut(s) 1087, 1486, 1599, 1972
MabI ACCWGGT 2 cut(s) 916, 1396
MaeI CTAG 2 cut(s) 257, 1815
MaeII ACGT 1 cut(s) 1459
MaeIII GTNAC 4 cut(s) 781, 1285, 1798, 2035
MalI GATC 6 cut(s) 1416, 1464, 1490, 1511, 1623, 1898
MbiI CCGCTC 1 cut(s) 1507
MboI GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
MflI RGATCY 1 cut(s) 1488
MhlI GDGCHC 2 cut(s) 729, 914
MlsI TGGCCA 2 cut(s) 566, 1656
MluCI AATT 8 cut(s) 111, 634, 755, 818, 1198, 1380, 1562, 1997
MluNI TGGCCA 2 cut(s) 566, 1656
Mly113I GGCGCC 1 cut(s) 879
MlyI GAGTC 3 cut(s) 471, 1692, 1712
MmeI TCCRAC 4 cut(s) 57, 120, 325, 345
Mox20I TGGCCA 2 cut(s) 566, 1656
MroI TCCGGA 1 cut(s) 769
MroNI GCCGGC 2 cut(s) 953, 1661
MscI TGGCCA 2 cut(s) 566, 1656
MseI TTAA 3 cut(s) 191, 1161, 1320
MslI CAYNNNNRTG 1 cut(s) 1829
Msp20I TGGCCA 2 cut(s) 566, 1656
MspA1I CMGCKG 1 cut(s) 1168
Mva1269I GAATGC 1 cut(s) 1336
MvaI CCWGG 6 cut(s) 380, 702, 719, 918, 1398, 1730
MvnI CGCG 5 cut(s) 519, 945, 986, 1121, 1895
NaeI GCCGGC 2 cut(s) 955, 1663
NarI GGCGCC 1 cut(s) 879
NciI CCSGG 4 cut(s) 711, 925, 1266, 1267
NcoI CCATGG 2 cut(s) 729, 1959
NdeII GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
NgoMIV GCCGGC 2 cut(s) 953, 1661
NlaIII CATG 9 cut(s) 475, 571, 733, 1580, 1592, 1828, 1963, 2083, 2116
NlaIV GGNNCC 7 cut(s) 46, 186, 727, 880, 912, 922, 1522
NmeAIII GCCGAG 1 cut(s) 855
NmuCI GTSAC 4 cut(s) 781, 1285, 1798, 2035
NruI TCGCGA 1 cut(s) 1895
NspI RCATGY 1 cut(s) 1592
PaeI GCATGC 1 cut(s) 1592
PaeR7I CTCGAG 1 cut(s) 1700
PagI TCATGA 2 cut(s) 471, 2112
PaqCI CACCTGC 1 cut(s) 1321
PceI AGGCCT 1 cut(s) 1300
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 3 cut(s) 585, 1067, 1491
PctI GAATGC 1 cut(s) 1336
PdiI GCCGGC 2 cut(s) 955, 1663
PfeI GAWTC 4 cut(s) 179, 602, 1247, 1735
Ple19I CGATCG 1 cut(s) 1899
PleI GAGTC 3 cut(s) 470, 1692, 1711
PluTI GGCGCC 1 cut(s) 882
PpsI GAGTC 3 cut(s) 470, 1692, 1711
PpuMI RGGWCCY 1 cut(s) 1520
PshAI GACNNNNGTC 1 cut(s) 1702
PshBI ATTAAT 1 cut(s) 1161
Psp5II RGGWCCY 1 cut(s) 1520
Psp6I CCWGG 6 cut(s) 378, 700, 717, 916, 1396, 1728
PspEI GGTNACC 1 cut(s) 1285
PspFI CCCAGC 3 cut(s) 306, 962, 2070
PspGI CCWGG 6 cut(s) 378, 700, 717, 916, 1396, 1728
PspN4I GGNNCC 7 cut(s) 46, 186, 727, 880, 912, 922, 1522
PspOMI GGGCCC 2 cut(s) 725, 910
PspPPI RGGWCCY 1 cut(s) 1520
PspXI VCTCGAGB 1 cut(s) 1700
PstI CTGCAG 3 cut(s) 831, 1332, 1422
PstNI CAGNNNCTG 3 cut(s) 1171, 1412, 1426
PsuI RGATCY 1 cut(s) 1488
PvuI CGATCG 1 cut(s) 1899
PvuII CAGCTG 1 cut(s) 1168
RruI TCGCGA 1 cut(s) 1895
RsaI GTAC 4 cut(s) 297, 866, 922, 1035
RsaNI GTAC 4 cut(s) 296, 865, 921, 1034
RseI CAYNNNNRTG 1 cut(s) 1829
SalI GTCGAC 1 cut(s) 1292
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 3 cut(s) 191, 1161, 1320
Sau3AI GATC 6 cut(s) 1414, 1462, 1488, 1509, 1621, 1896
SbfI CCTGCAGG 1 cut(s) 1332
SchI GAGTC 3 cut(s) 471, 1692, 1712
SdaI CCTGCAGG 1 cut(s) 1332
SduI GDGCHC 2 cut(s) 729, 914
SexAI ACCWGGT 2 cut(s) 916, 1396
SfaNI GCATC 4 cut(s) 1087, 1486, 1599, 1972
SfcI CTRYAG 4 cut(s) 418, 827, 1328, 1418
SfoI GGCGCC 1 cut(s) 880
Sfr274I CTCGAG 1 cut(s) 1700
SinI GGWCC 2 cut(s) 744, 1520
SlaI CTCGAG 1 cut(s) 1700
SmaI CCCGGG 1 cut(s) 1267
SmiMI CAYNNNNRTG 1 cut(s) 1829
SmlI CTYRAG 1 cut(s) 1700
SmoI CTYRAG 1 cut(s) 1700
SphI GCATGC 1 cut(s) 1592
Sse8387I CCTGCAGG 1 cut(s) 1332
Sse9I AATT 8 cut(s) 111, 634, 755, 818, 1198, 1380, 1562, 1997
SseBI AGGCCT 1 cut(s) 1300
SspDI GGCGCC 1 cut(s) 878
SspMI CTAG 2 cut(s) 257, 1815
StuI AGGCCT 1 cut(s) 1300
StyI CCWWGG 3 cut(s) 729, 1959, 2025
TaaI ACNGT 6 cut(s) 55, 482, 1291, 1718, 2095, 2102
TaiI ACGT 1 cut(s) 1462
TaqII GACCGA 1 cut(s) 1293
TasI AATT 8 cut(s) 111, 634, 755, 818, 1198, 1380, 1562, 1997
TatI WGTACW 1 cut(s) 864
TauI GCSGC 9 cut(s) 224, 289, 329, 516, 519, 717, 989, 1134, 1223
TfiI GAWTC 4 cut(s) 179, 602, 1247, 1735
Tru1I TTAA 3 cut(s) 191, 1161, 1320
Tru9I TTAA 3 cut(s) 191, 1161, 1320
TscAI CASTG 3 cut(s) 394, 505, 1693
TseFI GTSAC 4 cut(s) 781, 1285, 1798, 2035
TseI GCWGC 6 cut(s) 200, 405, 826, 1168, 1439, 1451
Tsp45I GTSAC 4 cut(s) 781, 1285, 1798, 2035
TspDTI ATGAA 7 cut(s) 460, 488, 584, 1072, 1445, 1813, 2101
TspGWI ACGGA 2 cut(s) 577, 1190
TspMI CCCGGG 1 cut(s) 1265
TspRI CASTG 3 cut(s) 394, 505, 1693
VpaK11BI GGWCC 2 cut(s) 744, 1520
VspI ATTAAT 1 cut(s) 1161
XapI RAATTY 1 cut(s) 755
XceI RCATGY 1 cut(s) 1592
XhoI CTCGAG 1 cut(s) 1700
XmaI CCCGGG 1 cut(s) 1265
XmiI GTMKAC 1 cut(s) 1293
XspI CTAG 2 cut(s) 257, 1815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.