Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001746

Physical Location & Seq

Genomic Coordinates Forward (+)

818733 .. 830500

Gene Structure 11768 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1010800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3831 bp

ATGGAGATCAACAGCCCAGCACCGGGTCCAAACACGGTGACGGTTCGCCGTAACCCTCACCGGAGGGCCAGGCCAACGCCAACGCCCGCCACAGCGGCTCGAGAAATGCAAATTCCGTCTTCAATTGCGCCCGGAGTACGATCTTTCCCAACCCAAGAAATCCTCTCCATGGACGTTGCTAAAACGAACCCTGTGGCCGAAAACCTTAGGGTTTTTCTGAGGATTCGGCCCCTTGTGCCGTGCAAGCTTGGGGACAAAACAGGCGGCTTCGGGGCGGATCAGAATCCGAAGCCGAGATTAAAAAATGCTTGGCCCCAAAACCCGGCGAAGAAGAAGTCGGCGGCCGGACGATCGCCGCAGATCAACAGAAAGATAAAGGAAGACGTCTGTATAAAGGTGAATACTTCTCATTCAGTGACGCTATCGCCGCCACTAGCACTGCAGGAATCTAACCGCACGAAGACTGAGGTTTATGAGGGGTTTTCCCACGTCTTCTCCTCTGATTCCTCTCAGGAAGAGGTGTATGATAAGATGGTGAAGCCTTTAGTAGAAGAATTTCTGAGGGGTAAGAGTGGGATGCTGGCTGCATTGGGGCCTAGTGGCTCGGGTAAGACGCATACGGTGTTTGGGTGCCCCAGGCAGCCGGGTATGGTTCCGCTTGCTCTTCAACATATCTTCAATCAGACTTCAGGGAGCATTTCTGAGTCTTCGAGATCATTTTTCATATCGATCTTCGAGATAAGCTCTGAACGAGGCAAAGGAGAAAGGCTATTTGACTTATCTCCTAATGGCGGAGATTTAAGCATGCAACAATCAACCCTAAGAGGCTTGCAAGAGATACCCATTTCTGATGCTAGACAGGCAGAGTCGTTAATTGCTCAGGCAATGCTAAAGCGTGCAACGGGAATGACAAATGCAAATAGCCAATCAAGCCGGTCACAGTGTATTATAAACATTCGCGCTGTTGCTGACAAGTGTAATGGAGACGAGAATAATCAAGCAAATGCAAATGATGGTGTCCTCACTATTGTTGATCTTGCTGGAGCGGAAAGAGAAAAAAGAACTGGAAATCAGGGAGTCAGATTGCTCGAAAGTAATTTCATCAACAATACGTCAATGGTTATAGGCTTGTGCTTGAGATCGTTACTCGAGCATCAGAAGAACCCTAAGAAGCCATTGCAGAAACACTTTCAGAACTCTTTGTTAACCAAGTATCTGCGAGATTATTTGGAAGGCAAGAAGAGGATGGCATTGATTTTAACAGTCAAATCAGGAGAAGAGGATTATCGTGATACATCTTACGTGCTAAGACAAGCCTCACCTTTTATGGAAATCAAGTATAATTATGTTGAAGAACCATCAAATATCTCATACCCCAAAAGGCATTTCCAAGCTTTGTCTAGAACTGAGCAGCGCAAAAAAATGAAAGTTACCGATCCCGATGCTTGCACGATTGAAGAACGCAAAAGCATTGAAGCTGAAAATGCACTATCCAAGGAAGGTTCTGTAAAAAGCAGTGGTTGCGTTGACTCCTATCAGGGAGAGAGAAACCATCGGATAATGCAGAGTTTTTCCAAAGCTATATGGAATGTTTTGAAGCAGTATAAAGAAAAACTCAAGGTGGCAGATGACGAAATCCAACATCTCAGACAAAACCTCATGACTGAACATACAAGATGTCTTGAGCTTGAAAAGGAGCTCAAGGATGTCCAGTCCTGCTGTTCATGTTCCAACAGAGAAGCAGTTGAGGTCAGCTTGTCCGGAGTGGGTGGTCAAGAATCTAGCAATCTTTATGAGGTGAAAAGTTTGGATGTTGGGGAGGTGAGTACTTTATGCCTTGAACTTGAAAACCCAAATCAGCAAGATCATCAGACATGTGAGAGAGTTTCCGGCAGTGAACATAGTGCCACAGATCTGTGTGAGTCTATATCTGTCAGACTGGAGGATAGTTATTTAAGTGTGGATGTAGCAGTGGACTCTTATCAGTCACTTGCCATGCATAGAAAGGATAGCTGCTCTTCTGTTGAACTGGACGACTTGCTTAGTGAGGGGCATAAGGAATCATCAAAAGATATTTCCTTAAATGGATATTGCAATGCTGTTGACCTGCTAGATGGTGAGAGTGCACTTGATACTTCTTGCCAAGTATTACAGACGGAGAACTTGGTGGAGGTTGCCTCTACTGGACAATGTAATGGTGTTGACCTGCTAGATTGTGAGAGTGGACTTGATACTTCTTGCCAAGCTATGCAATTGAAGAAATCGGAAGATGCCCCCTCTACTGGACATTGCAATGCTGTTGACCTGGTAGATGGTGAGAGTTTACTCAATGCTTCTTGCCAAGCATTACAGACGGAGAACTTGGAGGAGGTTGCCTTGACTGGACAAAGTAATGGTGTTGACCTGCTAGCTTGTGAGAGTGGCCTTGATACTTCTTGCCGAGCTATACGGTTGACGAAATCGGAAGCTATACAGTTGAAGAAATCGGAAGATGTCCCCTCTACTGGACATTGCAATGCTGTTGACCTGCTAGATGGTGAAAGTTCACTCGATACTTCTTGCCAAGCATTACGGATGGAGAACTTGGAGGAGGTTGCCTCTACTGGACAAAGTAATGGTGTTGACCTGCTAGATTGTGAGAGTGGCCTTGATACTTCTTCCCAAGCTATACGGTTGAAGAAATTGGAAGATGTCCCCTCTACTGGACTTTACAATTCTGTTGACCTGCTAGATGGTGAGAGTGCACTTGATACTTCTTGCCAAGTATTACAGACGGAGAACTTGGTGGAGGTTGCCTCTACTGGACAATGTAATGGTGTTGACCTGCTAGATTGTGAGAGTGGACTTGATACTTCTTGCCAAGCTATGCAATTGAAGAAATCGGAAGATGCCCCCTCTACTGGACATTGCATTGCTGTTGACCTGCTAGATGGTGAGAGTGCACTCGATACTTCTTGCCAAGCATTACAGACGGAGAACTTGGAGGAGGTTGCCTTGACTGGACAATGTAATGGTGTTGACCTGCTAGATTGTGAGAGTGGCCTTGATACCTCTTGCCAAGCTATACGGTGGGAGAAATCAGAATGTGTCCTCTCTACTGGACATTATAATGTTGTTAGCCAGCTAGATTGTGAGAGTGGACTTGATACTTCTTGCCAAGCTTTACAGTTGGAGAAATCGGAAAGGAGAGCTTCGCCTTCTTCATCTCCATCACCAAAGGATTTCAGTGCGGTGGATGTTGAGGATGAGATTCAGAAACCTCGGGAATTATTGAACTTTCCAACATCACCGAAGGTAGACCTTGTGTCAACTAAAGGGTGCGATGTTATTGAGATTCCAGACAGTAAACCAAGAGAATCCCGCGTTTTGGATGCGAAGGATGAGATTCAGAAGCCTTGGGAATTATTGAACTTTCCAACATCTCTGAAGGAAGACCTTGTGTCAACTAAAGGGTGCGACGTTATTGAGATTCCTGACAGTGAACCAAGGGACTCCGTTGTTTTGGATGCGAAGGGTGAGATTGAGAAGCCACAGACGTTATTAAATGTTCCTACATCACCGAAGGAAGACCTTGTGTCAACTAAAGGGCGCGATGCCATTGATGTTACTGACAGTGAACCGGGTGTTAAAATCTCTACAAAAACAGAGAAACCGAAAAGGAGACTTTTGCCTGCATCATCCTTATTACTAAGGGATTTCAGTGCTCTGGACATTGACGATGATGAGAAGCCAAAGCGTGGAAAGAAGAAATCAGGTGCTGTTGAAGAAAGGCACAGAACGCAGGGTAGCATCTCTCTTTTGCGTTTGCTCCACAGAAATCTTCCTAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008150 GO:0008574 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048285 GO:0051225 GO:0051231 GO:0070925 GO:0071840 GO:0090307 GO:0098813 GO:0140014 GO:1902850 GO:1903047 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1277

Amino Acids

139.48

Weight (kDa)

5.01

Isoelectric Point (pI)

47.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	134 - 232	2.8e-08	Microtubule binding
Kinesin	PF00225	145 - 442	6.5e-52	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0001746)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060
fragaria_vesca	FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_4g19391 FvH4_5g38380
malus_domestica	MD08G1010800.v1.1
prunus_persica	Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.8G272100_v2.0.a1 Prupe.8G272100_v2.0.a1
pyrus_communis	pycom08g00960
rosa_chinensis	RchiOBHm_Chr6g0303131
rosa_laevigata	RLG00000011105
rosa_multiflora	Rmu_co8047954.1_g000001 Rmu_sc0001706.1_g000038 Rmu_sc0028461.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265850 Rroxscaffold_7G00164990
rosa_rugosa	Rorug06G0319300
rosa_samantha	Rh6AG433300 Rh6BG469400 Rh6CG445600 Rh6DG432400
rosa_wichuraiana	Rw6G037570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	950, 3108
AatII	GACGTC	1 cut(s)	387
Acc36I	ACCTGC	9 cut(s)	2115, 2214, 2412, 2535, 2634, 2733, 2832, 2931, 3030
AccB1I	GGYRCC	1 cut(s)	630
AccB7I	CCANNNNNTGG	1 cut(s)	3737
AccBSI	CCGCTC	1 cut(s)	1046
AccI	GTMKAC	1 cut(s)	3297
AccII	CGCG	3 cut(s)	960, 3363, 3591
AccIII	TCCGGA	1 cut(s)	1760
AclWI	GGATC	2 cut(s)	285, 1430
AcoI	YGGCCR	2 cut(s)	195, 342
AcsI	RAATTY	2 cut(s)	111, 554
AcuI	CTGAAG	2 cut(s)	672, 3446
AcyI	GRCGYC	1 cut(s)	384
AfaI	GTAC	2 cut(s)	138, 1828
AflIII	ACRYGT	1 cut(s)	1874
AhdI	GACNNNNNGTC	3 cut(s)	3304, 3439, 3574
AjiI	CACGTC	1 cut(s)	490
AjnI	CCWGG	3 cut(s)	68, 635, 2304
AjuI	GAANNNNNNNTTGG	2 cut(s)	1370, 1402
Alw21I	GWGCWC	5 cut(s)	1701, 2128, 2746, 2944, 3706
Alw26I	GTCTC	2 cut(s)	978, 3655
Alw44I	GTGCAC	3 cut(s)	2124, 2742, 2940
AlwI	GGATC	2 cut(s)	285, 1430
AlwNI	CAGNNNCTG	1 cut(s)	3758
Ama87I	CYCGRG	4 cut(s)	99, 604, 1148, 3261
Aor13HI	TCCGGA	1 cut(s)	1760
ApaLI	GTGCAC	3 cut(s)	2124, 2742, 2940
ApeKI	GCWGC	4 cut(s)	584, 640, 1411, 2013
ApoI	RAATTY	2 cut(s)	111, 554
ArsI	GACNNNNNNTTYG	2 cut(s)	320, 352
Asp700I	GAANNNNTTC	1 cut(s)	555
AspLEI	GCGC	4 cut(s)	130, 962, 1416, 3591
AspS9I	GGNCC	5 cut(s)	26, 66, 228, 312, 593
AsuC2I	CCSGG	5 cut(s)	24, 132, 323, 645, 3621
AsuNHI	GCTAGC	1 cut(s)	2407
AvaI	CYCGRG	4 cut(s)	99, 604, 1148, 3261
AvaII	GGWCC	1 cut(s)	26
AxyI	CCTNAGG	1 cut(s)	206
BaeGI	GKGCMC	4 cut(s)	635, 2128, 2746, 2944
BanI	GGYRCC	1 cut(s)	630
BanII	GRGCYC	1 cut(s)	1701
BbsI	GAAGAC	7 cut(s)	111, 387, 467, 484, 699, 3438, 3573
Bbv12I	GWGCWC	5 cut(s)	1701, 2128, 2746, 2944, 3706
BbvI	GCAGC	4 cut(s)	571, 652, 1423, 2000
BceAI	ACGGC	2 cut(s)	33, 223
BcgI	CGANNNNNNTGC	2 cut(s)	1424, 1458
BciT130I	CCWGG	3 cut(s)	70, 637, 2306
BcnI	CCSGG	5 cut(s)	24, 132, 323, 645, 3621
BcoDI	GTCTC	2 cut(s)	978, 3655
BfmI	CTRYAG	1 cut(s)	440
BfuAI	ACCTGC	9 cut(s)	2115, 2214, 2412, 2535, 2634, 2733, 2832, 2931, 3030
BglI	GCCNNNNNGGC	1 cut(s)	95
BglII	AGATCT	1 cut(s)	1912
BisI	GCNGC	9 cut(s)	96, 265, 342, 356, 428, 585, 641, 1412, 2014
BlsI	GCNGC	9 cut(s)	97, 266, 343, 357, 429, 586, 642, 1413, 2015
BmcAI	AGTACT	1 cut(s)	1828
Bme1390I	CCNGG	8 cut(s)	24, 70, 132, 323, 637, 645, 2306, 3621
Bme18I	GGWCC	1 cut(s)	26
BmeRI	GACNNNNNGTC	3 cut(s)	3304, 3439, 3574
BmeT110I	CYCGRG	4 cut(s)	99, 604, 1148, 3261
BmgBI	CACGTC	1 cut(s)	490
BmgT120I	GGNCC	5 cut(s)	26, 66, 228, 312, 593
BmiI	GGNNCC	6 cut(s)	27, 230, 314, 594, 632, 654
BmrFI	CCNGG	8 cut(s)	24, 70, 132, 323, 637, 645, 2306, 3621
BmtI	GCTAGC	1 cut(s)	2411
BpiI	GAAGAC	7 cut(s)	111, 387, 467, 484, 699, 3438, 3573
BplI	GAGNNNNNCTC	8 cut(s)	728, 760, 2162, 2194, 2582, 2614, 2780, 2812
BpmI	CTGGAG	2 cut(s)	1062, 1961
Bpu10I	CCTNAGC	1 cut(s)	879
BpuEI	CTTGAG	4 cut(s)	1156, 1601, 1685, 1703
BpuMI	CCSGG	5 cut(s)	24, 132, 323, 645, 3621
Bsa29I	ATCGAT	1 cut(s)	728
BsaAI	YACGTR	1 cut(s)	1303
BsaBI	GATNNNNATC	1 cut(s)	282
BsaHI	GRCGYC	1 cut(s)	384
BsaJI	CCNNGG	6 cut(s)	168, 635, 1494, 3260, 3395, 3485
BsaWI	WCCGGW	2 cut(s)	60, 1760
BsaXI	ACNNNNNCTCC	2 cut(s)	479, 509
Bse118I	RCCGGY	1 cut(s)	933
Bse21I	CCTNAGG	1 cut(s)	206
Bse3DI	GCAATG	9 cut(s)	891, 1175, 2101, 2287, 2299, 2509, 2521, 2905, 2910
Bse8I	GATNNNNATC	1 cut(s)	282
BseAI	TCCGGA	1 cut(s)	1760
BseBI	CCWGG	3 cut(s)	70, 637, 2306
BseCI	ATCGAT	1 cut(s)	728
BseDI	CCNNGG	6 cut(s)	168, 635, 1494, 3260, 3395, 3485
BseJI	GATNNNNATC	1 cut(s)	282
BseMI	GCAATG	9 cut(s)	891, 1175, 2101, 2287, 2299, 2509, 2521, 2905, 2910
BseMII	CTCAG	8 cut(s)	209, 456, 524, 551, 693, 893, 1398, 1660
BseRI	GAGGAG	4 cut(s)	487, 2381, 2603, 2999
BseSI	GKGCMC	4 cut(s)	635, 2128, 2746, 2944
BseX3I	CGGCCG	1 cut(s)	342
BseXI	GCAGC	4 cut(s)	571, 652, 1423, 2000
BseYI	CCCAGC	1 cut(s)	16
Bsh1236I	CGCG	3 cut(s)	960, 3363, 3591
Bsh1285I	CGRYCG	2 cut(s)	345, 353
BshNI	GGYRCC	1 cut(s)	630
BshVI	ATCGAT	1 cut(s)	728
BsiEI	CGRYCG	2 cut(s)	345, 353
BsiHKAI	GWGCWC	5 cut(s)	1701, 2128, 2746, 2944, 3706
BsiHKCI	CYCGRG	4 cut(s)	99, 604, 1148, 3261
BslFI	GGGAC	4 cut(s)	266, 2480, 2678, 3503
BsmAI	GTCTC	2 cut(s)	978, 3655
BsmBI	CGTCTC	1 cut(s)	978
BsmFI	GGGAC	4 cut(s)	266, 2480, 2678, 3503
BsoBI	CYCGRG	4 cut(s)	99, 604, 1148, 3261
Bsp1286I	GDGCHC	6 cut(s)	635, 1701, 2128, 2746, 2944, 3706
Bsp13I	TCCGGA	1 cut(s)	1760
Bsp19I	CCATGG	1 cut(s)	168
BspCNI	CTCAG	8 cut(s)	210, 457, 523, 552, 694, 892, 1399, 1659
BspDI	ATCGAT	1 cut(s)	728
BspEI	TCCGGA	1 cut(s)	1760
BspFNI	CGCG	3 cut(s)	960, 3363, 3591
BspHI	TCATGA	1 cut(s)	1659
BspLI	GGNNCC	6 cut(s)	27, 230, 314, 594, 632, 654
BspMAI	CTGCAG	1 cut(s)	444
BspMI	ACCTGC	9 cut(s)	2115, 2214, 2412, 2535, 2634, 2733, 2832, 2931, 3030
BspOI	GCTAGC	1 cut(s)	2411
BspPI	GGATC	2 cut(s)	285, 1430
BspQI	GCTCTTC	2 cut(s)	669, 2023
BspT107I	GGYRCC	1 cut(s)	630
BsrBI	CCGCTC	1 cut(s)	1046
BsrDI	GCAATG	9 cut(s)	891, 1175, 2101, 2287, 2299, 2509, 2521, 2905, 2910
BsrFI	RCCGGY	1 cut(s)	933
BssAI	RCCGGY	1 cut(s)	933
BssECI	CCNNGG	6 cut(s)	168, 635, 1494, 3260, 3395, 3485
BssNI	GRCGYC	1 cut(s)	384
BssT1I	CCWWGG	4 cut(s)	168, 1494, 3395, 3485
Bst2UI	CCWGG	3 cut(s)	70, 637, 2306
Bst6I	CTCTTC	5 cut(s)	510, 669, 1235, 1272, 2023
BstACI	GRCGYC	1 cut(s)	384
BstAPI	GCANNNNNTGC	1 cut(s)	1521
BstBAI	YACGTR	1 cut(s)	1303
BstDSI	CCRYGG	1 cut(s)	168
BstFNI	CGCG	3 cut(s)	960, 3363, 3591
BstHHI	GCGC	4 cut(s)	130, 962, 1416, 3591
BstMAI	GTCTC	2 cut(s)	978, 3655
BstMCI	CGRYCG	2 cut(s)	345, 353
BstMWI	GCNNNNNNNGC	9 cut(s)	95, 235, 244, 427, 860, 930, 1484, 1521, 3778
BstNI	CCWGG	3 cut(s)	70, 637, 2306
BstNSI	RCATGY	2 cut(s)	808, 1878
BstSCI	CCNGG	8 cut(s)	22, 68, 130, 321, 635, 643, 2304, 3619
BstSFI	CTRYAG	1 cut(s)	440
BstSLI	GKGCMC	4 cut(s)	635, 2128, 2746, 2944
BstUI	CGCG	3 cut(s)	960, 3363, 3591
BstV1I	GCAGC	4 cut(s)	571, 652, 1423, 2000
BstV2I	GAAGAC	7 cut(s)	111, 387, 467, 484, 699, 3438, 3573
BstX2I	RGATCY	1 cut(s)	1912
BstYI	RGATCY	1 cut(s)	1912
BstZI	CGGCCG	1 cut(s)	342
Bsu15I	ATCGAT	1 cut(s)	728
Bsu36I	CCTNAGG	1 cut(s)	206
BsuTUI	ATCGAT	1 cut(s)	728
BtgI	CCRYGG	1 cut(s)	168
BtgZI	GCGATG	2 cut(s)	3336, 3606
BtrI	CACGTC	1 cut(s)	490
BtsI	GCAGTG	4 cut(s)	437, 1522, 1900, 1977
BveI	ACCTGC	9 cut(s)	2115, 2214, 2412, 2535, 2634, 2733, 2832, 2931, 3030
CaiI	CAGNNNCTG	1 cut(s)	3758
CciI	TCATGA	1 cut(s)	1659
CfoI	GCGC	4 cut(s)	130, 962, 1416, 3591
Cfr10I	RCCGGY	1 cut(s)	933
Cfr13I	GGNCC	5 cut(s)	26, 66, 228, 312, 593
ClaI	ATCGAT	1 cut(s)	728
CseI	GACGC	2 cut(s)	427, 622
CsiI	ACCWGGT	1 cut(s)	2304
Csp6I	GTAC	2 cut(s)	137, 1827
CviAII	CATG	6 cut(s)	169, 805, 1660, 1725, 1875, 1996
CviQI	GTAC	2 cut(s)	137, 1827
DriI	GACNNNNNGTC	3 cut(s)	3304, 3439, 3574
EaeI	YGGCCR	2 cut(s)	195, 342
EagI	CGGCCG	1 cut(s)	342
Eam1104I	CTCTTC	5 cut(s)	510, 669, 1235, 1272, 2023
Eam1105I	GACNNNNNGTC	3 cut(s)	3304, 3439, 3574
EarI	CTCTTC	5 cut(s)	510, 669, 1235, 1272, 2023
EciI	GGCGGA	2 cut(s)	290, 807
Ecl136II	GAGCTC	1 cut(s)	1699
EclXI	CGGCCG	1 cut(s)	342
Eco130I	CCWWGG	4 cut(s)	168, 1494, 3395, 3485
Eco24I	GRGCYC	1 cut(s)	1701
Eco47I	GGWCC	1 cut(s)	26
Eco52I	CGGCCG	1 cut(s)	342
Eco53kI	GAGCTC	1 cut(s)	1699
Eco57I	CTGAAG	2 cut(s)	672, 3446
Eco81I	CCTNAGG	1 cut(s)	206
Eco88I	CYCGRG	4 cut(s)	99, 604, 1148, 3261
EcoICRI	GAGCTC	1 cut(s)	1699
EcoO109I	RGGNCCY	1 cut(s)	593
EcoRII	CCWGG	3 cut(s)	68, 635, 2304
EcoT14I	CCWWGG	4 cut(s)	168, 1494, 3395, 3485
EcoT22I	ATGCAT	1 cut(s)	2001
EcoT38I	GRGCYC	1 cut(s)	1701
ErhI	CCWWGG	4 cut(s)	168, 1494, 3395, 3485
Esp3I	CGTCTC	1 cut(s)	978
FaeI	CATG	6 cut(s)	172, 808, 1663, 1728, 1878, 1999
FalI	AAGNNNNNCTT	2 cut(s)	3175, 3207
FaqI	GGGAC	4 cut(s)	266, 2480, 2678, 3503
FatI	CATG	6 cut(s)	168, 804, 1659, 1724, 1874, 1995
FauI	CCCGC	2 cut(s)	94, 3368
FblI	GTMKAC	1 cut(s)	3297
Fnu4HI	GCNGC	9 cut(s)	96, 265, 342, 356, 428, 585, 641, 1412, 2014
FriOI	GRGCYC	1 cut(s)	1701
Fsp4HI	GCNGC	9 cut(s)	96, 265, 342, 356, 428, 585, 641, 1412, 2014
GlaI	GCGC	4 cut(s)	129, 961, 1415, 3590
GluI	GCNGC	9 cut(s)	96, 265, 342, 356, 428, 585, 641, 1412, 2014
GsaI	CCCAGC	1 cut(s)	20
GsuI	CTGGAG	2 cut(s)	1062, 1961
HgaI	GACGC	2 cut(s)	427, 622
HhaI	GCGC	4 cut(s)	130, 962, 1416, 3591
Hin1I	GRCGYC	1 cut(s)	384
Hin1II	CATG	6 cut(s)	172, 808, 1663, 1728, 1878, 1999
Hin6I	GCGC	4 cut(s)	128, 960, 1414, 3589
HinP1I	GCGC	4 cut(s)	128, 960, 1414, 3589
HindIII	AAGCTT	3 cut(s)	245, 1392, 3159
HpaI	GTTAAC	1 cut(s)	1206
Hpy99I	CGWCG	1 cut(s)	3461
HpyAV	CCTTC	8 cut(s)	1226, 1493, 3207, 3286, 3370, 3421, 3505, 3556
HpyCH4IV	ACGT	7 cut(s)	174, 384, 489, 1112, 1302, 3459, 3536
HpyF10VI	GCNNNNNNNGC	9 cut(s)	95, 235, 244, 427, 860, 930, 1484, 1521, 3778
HpySE526I	ACGT	7 cut(s)	174, 384, 489, 1112, 1302, 3459, 3536
Hsp92I	GRCGYC	1 cut(s)	384
Hsp92II	CATG	6 cut(s)	172, 808, 1663, 1728, 1878, 1999
HspAI	GCGC	4 cut(s)	128, 960, 1414, 3589
Kpn2I	TCCGGA	1 cut(s)	1760
KspAI	GTTAAC	1 cut(s)	1206
LguI	GCTCTTC	2 cut(s)	669, 2023
LmnI	GCTCC	4 cut(s)	693, 1043, 1696, 3813
Lsp1109I	GCAGC	4 cut(s)	571, 652, 1423, 2000
MabI	ACCWGGT	1 cut(s)	2304
MaeII	ACGT	7 cut(s)	174, 384, 489, 1112, 1302, 3459, 3536
MaeIII	GTNAC	8 cut(s)	37, 50, 415, 936, 1143, 1429, 1986, 3604
MbiI	CCGCTC	1 cut(s)	1046
MfeI	CAATTG	3 cut(s)	123, 2252, 2870
MflI	RGATCY	1 cut(s)	1912
MhlI	GDGCHC	6 cut(s)	635, 1701, 2128, 2746, 2944, 3706
MlyI	GAGTC	7 cut(s)	713, 875, 1086, 1523, 1931, 1970, 3485
MmeI	TCCRAC	5 cut(s)	1663, 1755, 3150, 3305, 3440
Mph1103I	ATGCAT	1 cut(s)	2001
MroI	TCCGGA	1 cut(s)	1760
MroXI	GAANNNNTTC	1 cut(s)	555
MseI	TTAA	9 cut(s)	299, 800, 872, 1205, 1259, 1955, 2081, 3542, 3627
MslI	CAYNNNNRTG	3 cut(s)	2292, 2514, 3108
MspA1I	CMGCKG	1 cut(s)	95
MspR9I	CCNGG	8 cut(s)	24, 70, 132, 323, 637, 645, 2306, 3621
MunI	CAATTG	3 cut(s)	123, 2252, 2870
MvaI	CCWGG	3 cut(s)	70, 637, 2306
MvnI	CGCG	3 cut(s)	960, 3363, 3591
MwoI	GCNNNNNNNGC	9 cut(s)	95, 235, 244, 427, 860, 930, 1484, 1521, 3778
NciI	CCSGG	5 cut(s)	24, 132, 323, 645, 3621
NcoI	CCATGG	1 cut(s)	168
NheI	GCTAGC	1 cut(s)	2407
NlaIII	CATG	6 cut(s)	172, 808, 1663, 1728, 1878, 1999
NlaIV	GGNNCC	6 cut(s)	27, 230, 314, 594, 632, 654
NmeAIII	GCCGAG	2 cut(s)	318, 2465
NmuCI	GTSAC	4 cut(s)	37, 415, 936, 1986
NsiI	ATGCAT	1 cut(s)	2001
NspI	RCATGY	2 cut(s)	808, 1878
PaeI	GCATGC	1 cut(s)	808
PaeR7I	CTCGAG	2 cut(s)	99, 1148
PagI	TCATGA	1 cut(s)	1659
PciI	ACATGT	1 cut(s)	1874
PciSI	GCTCTTC	2 cut(s)	669, 2023
PdmI	GAANNNNTTC	1 cut(s)	555
PflMI	CCANNNNNTGG	1 cut(s)	3737
PkrI	GCNGC	9 cut(s)	97, 266, 343, 357, 429, 586, 642, 1413, 2015
Ple19I	CGATCG	1 cut(s)	353
PleI	GAGTC	7 cut(s)	712, 874, 1085, 1523, 1930, 1970, 3485
PpsI	GAGTC	7 cut(s)	712, 874, 1085, 1523, 1930, 1970, 3485
Ppu21I	YACGTR	1 cut(s)	1303
PscI	ACATGT	1 cut(s)	1874
PsiI	TTATAA	2 cut(s)	950, 3108
Psp124BI	GAGCTC	1 cut(s)	1701
Psp6I	CCWGG	3 cut(s)	68, 635, 2304
PspFI	CCCAGC	1 cut(s)	16
PspGI	CCWGG	3 cut(s)	68, 635, 2304
PspN4I	GGNNCC	6 cut(s)	27, 230, 314, 594, 632, 654
PspPI	GGNCC	5 cut(s)	26, 66, 228, 312, 593
PspXI	VCTCGAGB	1 cut(s)	1148
PstI	CTGCAG	1 cut(s)	444
PstNI	CAGNNNCTG	1 cut(s)	3758
PsuI	RGATCY	1 cut(s)	1912
PvuI	CGATCG	1 cut(s)	353
RsaI	GTAC	2 cut(s)	138, 1828
RsaNI	GTAC	2 cut(s)	137, 1827
RseI	CAYNNNNRTG	3 cut(s)	2292, 2514, 3108
SacI	GAGCTC	1 cut(s)	1701
SapI	GCTCTTC	2 cut(s)	669, 2023
SaqAI	TTAA	9 cut(s)	299, 800, 872, 1205, 1259, 1955, 2081, 3542, 3627
SatI	GCNGC	9 cut(s)	96, 265, 342, 356, 428, 585, 641, 1412, 2014
Sau96I	GGNCC	5 cut(s)	26, 66, 228, 312, 593
ScaI	AGTACT	1 cut(s)	1828
SchI	GAGTC	7 cut(s)	713, 875, 1086, 1523, 1931, 1970, 3485
ScrFI	CCNGG	8 cut(s)	24, 70, 132, 323, 637, 645, 2306, 3621
SduI	GDGCHC	6 cut(s)	635, 1701, 2128, 2746, 2944, 3706
SexAI	ACCWGGT	1 cut(s)	2304
SfcI	CTRYAG	1 cut(s)	440
Sfr274I	CTCGAG	2 cut(s)	99, 1148
SinI	GGWCC	1 cut(s)	26
SlaI	CTCGAG	2 cut(s)	99, 1148
SmiMI	CAYNNNNRTG	3 cut(s)	2292, 2514, 3108
SmlI	CTYRAG	6 cut(s)	99, 1135, 1148, 1616, 1682, 1700
SmoI	CTYRAG	6 cut(s)	99, 1135, 1148, 1616, 1682, 1700
SphI	GCATGC	1 cut(s)	808
SstI	GAGCTC	1 cut(s)	1701
StyD4I	CCNGG	8 cut(s)	22, 68, 130, 321, 635, 643, 2304, 3619
StyI	CCWWGG	4 cut(s)	168, 1494, 3395, 3485
TaiI	ACGT	7 cut(s)	177, 387, 492, 1115, 1305, 3462, 3539
TaqI	TCGA	8 cut(s)	100, 710, 728, 735, 1089, 1149, 2550, 2946
TatI	WGTACW	1 cut(s)	1826
TauI	GCSGC	5 cut(s)	98, 267, 344, 358, 430
Tru1I	TTAA	9 cut(s)	299, 800, 872, 1205, 1259, 1955, 2081, 3542, 3627
Tru9I	TTAA	9 cut(s)	299, 800, 872, 1205, 1259, 1955, 2081, 3542, 3627
TseFI	GTSAC	4 cut(s)	37, 415, 936, 1986
TseI	GCWGC	4 cut(s)	584, 640, 1411, 2013
Tsp45I	GTSAC	4 cut(s)	37, 415, 936, 1986
TspDTI	ATGAA	5 cut(s)	712, 1090, 1439, 1713, 3192
TspGWI	ACGGA	7 cut(s)	105, 2171, 2369, 2587, 2789, 2987, 3484
Van91I	CCANNNNNTGG	1 cut(s)	3737
VneI	GTGCAC	3 cut(s)	2124, 2742, 2940
VpaK11BI	GGWCC	1 cut(s)	26
XapI	RAATTY	2 cut(s)	111, 554
XbaI	TCTAGA	1 cut(s)	1400
XceI	RCATGY	2 cut(s)	808, 1878
XhoI	CTCGAG	2 cut(s)	99, 1148
XmiI	GTMKAC	1 cut(s)	3297
XmnI	GAANNNNTTC	1 cut(s)	555
ZraI	GACGTC	1 cut(s)	385
ZrmI	AGTACT	1 cut(s)	1828
Zsp2I	ATGCAT	1 cut(s)	2001

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.