Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001746

Physical Location & Seq

Genomic Coordinates Reverse (-)

62966616 .. 62973922

Gene Structure 7307 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ27230

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGGAGGAGCCTACAATCCTGCAGCAGTGTCCAAACACGGTCACCATCCGGCGCAACCCTCACCGGAGAGCGAGGCCCAAGACTACTCCGGCAACCGCAGCCGCCGGAATCCCGTCTTGTACTGTGCCGAAAGTCCCTTTCCCGACGGAAGAAATTCTCTCCATGGACATATCGAGCTCGAAACCTGACCCCGAGAATCTCAGAGTCTTCCTCCGAATTCGACCTCTACTGAGGCCGTCGGACAAAAGGAGCGGCGTCGTCGGAGATTTAAAGGCCAGATCCAAAAACGTTTGGCCGCAAAACCCTAAGAACGCCGCGGCGAGGGAACAGAAGAAGAAGAAAAGCCCTAACGCCGAGGTCTGTGTCAAGGTGAACAGTGCTCGCTCGGTGACTGTGGCGCCGCCACTATCCTTGCAGGAACCTAACCGGCTCAAGTCTGAGGTCTACGATGGCTTCTCACACGTCTTCTCCTCCGATTCTTCTCAGGAGGAGGTGTATGAGATGATGGTGAAGCCATTGGTGGAAGACTTCTTGAAGGGCAAGAGTGGAATGTTGGCTGCAATGGGGCCTAGTGGCTCCGGCAAGACTCATACAGTTTTCGGTTGCCCCAGGGAGCCCGGAATGGTACCACTTGCGCTTCAACACATATTCAAGCGAGTTCCGGATAGCATTACCGAGTCTCCGAGGTCATTTTTCATATCAATGTTTGAAATAAGTACTGAACGAGGTAAAGCAGAGAAATGGATTGATTTATCTCCAAATGGGGGTGAAATAACTATGCAACAATCTAACCTGAAAGGCCTACAAGAGATAGCCATATCTGATGCTCGACAGGCAGAATCCTTAGTTGCGCAGGCAATGCAAAAACGTGCCACTGCTACGACAAATTCAAACAGCCAGTCAAGTCGTTCACAGTGTATTATTAACATTCGAGGTGTTGCCGACAAGTTTGATGGAGAGGATAACAATAATGCAAATGAAGTTGTCCTAAGTATTGTTGACCTTGCTGGAGCTGAAAGAGAAAAGAGAACTGGGAATCAGGGAGCAAGGTTGCTTGAAAGTAATTTCATCAACAACACATCGATGGTTTTTGGCTTGTGCTTGAGATCCTTATTGGAGCATCAGAAGAACCCTAAGAAGCCAATGCAAAAGCACTTTCAGAACTCATTGTTGACCAAGTACTTGAGAGACTATTTGGAAGGCAAGAAGAGGATGTCACTGATTTTAACAGTAAAATCAGGAGAAGAAGATTATCGTGATACATCTCACCTGCTAAGACAGGCCTCACCTTATATGGAAATCAAGTTTAATAATGCTGAAGACCCTTCAAATATTCCGAACAAAAGGCAAATCCAGTCTTTGTCTAGAACTGAGCAGCGCAAAAGAATGAAATTAACCGGTTCTGAGTCTTGCGTGATTGAACAAGAGAAAAGCATTGAATCCGAAATTGGACTAACCAAGGAAGCGGCACAAAGAATCTACAAGGTGGAATTAAGTAGTGGTTCAAAACCGTGTAATGTTGACACTGCAGATGGAGAGACAAACCATCAGATTATGCAGAGTTTTGCTAAAGCTATATGGAATGTTTTGAAGGAGTACAACAAAAAACTAAAGGTGGCGGATGATGAAATCAAGCATCTCAGGGAAAGTCTTATGACTGAAAAAACAAGACATCTTGAGCTGGAAAAAGAGCTGAAGGATATAAAGTCCTGCTGTACATGCTTCCACATAAAATCCAATGTTGATCAGCCTTTCTACTCAGGGAGATGTGACAACAATGGCACTCCCAAAAGATTGGACTTTACTTCAAAAGAGGATCACAATATTTTCTCCGAGGTGCAGGCCTTTGGGTGTGTTGACAATGAACCCGATATTGACGCAACTCTAGTTCCAAGCCAGACTGATGAAAAACAAAGCGATGCTACTGTGAAAACTTCGGATGTTGGTGAGGTCGGTAATCCTTGCCTTGGACCTGGAAACACAGACCAGTGGAATCATCAGATATGTGAGGGCTTCCCTGGCAGTGGACATAGTGCGGAAGATGGGTGCGAGTCTAAATGTGTACAAATAGAGGATAATTATCCTAATGCAGATGTAGCAGGTCTTTCAGTGAACTCTTCTGAGCTGGTTGTTATTCAAAGAAAGGATAGCTGCTCATCAGTGGAGCTTGATGACCTGCTAAGTGAGGAGGATGAGGAATCGTCGGATGTTGTATATACTGAACATTGCAATAGCGTTGACCGGCAGTCTAGACTTGATACTTCTTCTGAGGCTTTGCATATGGACAAGTCAGAAAGGAAAGTGTTGCATGCGTCATCTCTATCGCCAAGTTCTAATGTTAAGAATGAGATTGAGGAGTCAAAAGAATCATTTGATTTGCCAACATTGCCGAAGGAAGGTGTTGTTTCAACTAAAAAGTGTAGCATTATTCATGTGCCTGTTAGTGAACCTACAGTTGATACATCTACCAAGCTGGAGAAACCAAAAAGGAGACTGTTGCCCGCCTCATCTACATTACTAAGAAATTTCAGTACTTCGGACATTGAGGATGACAGTGAGAAACCAAAGGGCGGAAAGAAATTAGCTGCAGAAGGAAGAACAAGAACGCAAGGTAGCATCTCTCTTGTGCGCTTGCTTCAGAGCAATCTCCGTATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0000280 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0006996 GO:0007010 GO:0007017 GO:0007018 GO:0007049 GO:0007051 GO:0007052 GO:0007059 GO:0008150 GO:0008574 GO:0009987 GO:0015630 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022402 GO:0022607 GO:0032991 GO:0042623 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048285 GO:0051225 GO:0051231 GO:0070925 GO:0071840 GO:0090307 GO:0098813 GO:0140014 GO:1902850 GO:1903047 GO:1990939

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

885

Amino Acids

98.31

Weight (kDa)

6.47

Isoelectric Point (pI)

56.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Kinesin	PF00225	119 - 430	4.8e-51	Kinesin motor domain
Microtub_bd	PF16796	119 - 222	1.2e-07	Microtubule binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001746)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060 AT1G20060
fragaria_vesca	FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_2g38240 FvH4_4g19391 FvH4_5g38380
malus_domestica	MD08G1010800.v1.1
prunus_persica	Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.1G363400_v2.0.a1 Prupe.8G272100_v2.0.a1 Prupe.8G272100_v2.0.a1
pyrus_communis	pycom08g00960
rosa_chinensis	RchiOBHm_Chr6g0303131
rosa_laevigata	RLG00000011105
rosa_multiflora	Rmu_co8047954.1_g000001 Rmu_sc0001706.1_g000038 Rmu_sc0028461.1_g000001
rosa_roxburghii	Rroxscaffold_3G00265850 Rroxscaffold_7G00164990
rosa_rugosa	Rorug06G0319300
rosa_samantha	Rh6AG433300 Rh6BG469400 Rh6CG445600 Rh6DG432400
rosa_wichuraiana	Rw6G037570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1278
Acc16I	TGCGCA	1 cut(s)	852
Acc36I	ACCTGC	3 cut(s)	1278, 2090, 2184
Acc65I	GGTACC	1 cut(s)	625
AccB1I	GGYRCC	2 cut(s)	397, 625
AccBSI	CCGCTC	1 cut(s)	252
AccI	GTMKAC	1 cut(s)	444
AccII	CGCG	1 cut(s)	317
AccIII	TCCGGA	1 cut(s)	661
AclI	AACGTT	1 cut(s)	288
AclWI	GGATC	3 cut(s)	273, 1101, 1824
AcoI	YGGCCR	1 cut(s)	293
AcsI	RAATTY	4 cut(s)	153, 216, 886, 2524
AcuI	CTGAAG	3 cut(s)	1338, 1715, 2621
AcyI	GRCGYC	2 cut(s)	255, 398
AfaI	GTAC	8 cut(s)	121, 627, 718, 1181, 1598, 1717, 2064, 2533
AfiI	CCNNNNNNNGG	6 cut(s)	321, 764, 1967, 2024, 2396, 2570
AflIII	ACRYGT	1 cut(s)	460
AgeI	ACCGGT	1 cut(s)	1397
AhdI	GACNNNNNGTC	1 cut(s)	2245
AjiI	CACGTC	1 cut(s)	463
AjnI	CCWGG	3 cut(s)	608, 1972, 2017
AjuI	GAANNNNNNNTTGG	2 cut(s)	1431, 1463
Alw21I	GWGCWC	2 cut(s)	179, 382
Alw26I	GTCTC	4 cut(s)	684, 1182, 1532, 2485
AlwI	GGATC	3 cut(s)	273, 1101, 1824
Ama87I	CYCGRG	1 cut(s)	191
Aor13HI	TCCGGA	1 cut(s)	661
AoxI	GGCC	8 cut(s)	74, 233, 273, 293, 566, 799, 1281, 1842
ApeKI	GCWGC	6 cut(s)	22, 98, 557, 1375, 2151, 2585
ApoI	RAATTY	4 cut(s)	153, 216, 886, 2524
ArsI	GACNNNNNNTTYG	2 cut(s)	884, 916
AsiGI	ACCGGT	1 cut(s)	1397
Asp700I	GAANNNNTTC	1 cut(s)	153
Asp718I	GGTACC	1 cut(s)	625
AspLEI	GCGC	6 cut(s)	54, 400, 637, 853, 1380, 2631
AspS9I	GGNCC	3 cut(s)	75, 566, 1970
AsuC2I	CCSGG	1 cut(s)	618
AsuHPI	GGTGA	9 cut(s)	34, 53, 382, 400, 520, 779, 1259, 1278, 1958
AvaI	CYCGRG	1 cut(s)	191
AvaII	GGWCC	1 cut(s)	1970
BanI	GGYRCC	2 cut(s)	397, 625
BanII	GRGCYC	2 cut(s)	179, 618
BbsI	GAAGAC	4 cut(s)	199, 457, 531, 1326
Bbv12I	GWGCWC	2 cut(s)	179, 382
BbvI	GCAGC	6 cut(s)	34, 110, 544, 1387, 2138, 2572
BccI	CCATC	8 cut(s)	53, 443, 499, 947, 1078, 1526, 1554, 2036
BceAI	ACGGC	1 cut(s)	220
BciT130I	CCWGG	3 cut(s)	610, 1974, 2019
BclI	TGATCA	1 cut(s)	1744
BcnI	CCSGG	1 cut(s)	618
BcoDI	GTCTC	4 cut(s)	684, 1182, 1532, 2485
BfaI	CTAG	4 cut(s)	570, 1365, 1886, 2250
BfmI	CTRYAG	4 cut(s)	20, 1527, 2451, 2586
BfoI	RGCGCY	1 cut(s)	401
BfuAI	ACCTGC	3 cut(s)	1278, 2090, 2184
BmcAI	AGTACT	3 cut(s)	718, 1181, 2533
Bme1390I	CCNGG	4 cut(s)	610, 618, 1974, 2019
Bme18I	GGWCC	1 cut(s)	1970
BmeRI	GACNNNNNGTC	1 cut(s)	2245
BmeT110I	CYCGRG	1 cut(s)	191
BmgBI	CACGTC	1 cut(s)	463
BmgT120I	GGNCC	3 cut(s)	75, 566, 1970
BmiI	GGNNCC	7 cut(s)	9, 399, 420, 567, 577, 615, 627
BmrFI	CCNGG	4 cut(s)	610, 618, 1974, 2019
BmrI	ACTGGG	1 cut(s)	1041
BmsI	GCATC	5 cut(s)	814, 1129, 1645, 1909, 2625
BmuI	ACTGGG	1 cut(s)	1041
BpiI	GAAGAC	4 cut(s)	199, 457, 531, 1326
BplI	GAGNNNNNCTC	2 cut(s)	195, 227
BpmI	CTGGAG	2 cut(s)	1029, 2495
BpuEI	CTTGAG	4 cut(s)	416, 1123, 1204, 1697
BpuMI	CCSGG	1 cut(s)	618
Bsa29I	ATCGAT	1 cut(s)	1082
BsaBI	GATNNNNATC	1 cut(s)	2079
BsaHI	GRCGYC	2 cut(s)	255, 398
BsaWI	WCCGGW	3 cut(s)	63, 661, 1397
BsaXI	ACNNNNNCTCC	6 cut(s)	452, 479, 482, 509, 664, 694
Bsc4I	CCNNNNNNNGG	6 cut(s)	321, 764, 1967, 2024, 2396, 2570
Bse118I	RCCGGY	3 cut(s)	426, 1397, 2241
Bse1I	ACTGG	4 cut(s)	898, 1036, 1354, 1987
Bse3DI	GCAATG	4 cut(s)	567, 864, 2224, 2384
Bse8I	GATNNNNATC	1 cut(s)	2079
BseAI	TCCGGA	1 cut(s)	661
BseBI	CCWGG	3 cut(s)	610, 1974, 2019
BseCI	ATCGAT	1 cut(s)	1082
BseGI	GGATG	7 cut(s)	45, 1218, 1627, 1945, 2197, 2212, 2554
BseJI	GATNNNNATC	1 cut(s)	2079
BseLI	CCNNNNNNNGG	6 cut(s)	321, 764, 1967, 2024, 2396, 2570
BseMI	GCAATG	4 cut(s)	567, 864, 2224, 2384
BseNI	ACTGG	4 cut(s)	898, 1036, 1354, 1987
BseRI	GAGGAG	5 cut(s)	20, 460, 503, 2201, 2369
BseXI	GCAGC	6 cut(s)	34, 110, 544, 1387, 2138, 2572
BsgI	GTGCAG	1 cut(s)	1859
Bsh1236I	CGCG	1 cut(s)	317
BshFI	GGCC	8 cut(s)	76, 235, 275, 295, 568, 801, 1283, 1844
BshNI	GGYRCC	2 cut(s)	397, 625
BshTI	ACCGGT	1 cut(s)	1397
BshVI	ATCGAT	1 cut(s)	1082
BsiHKAI	GWGCWC	2 cut(s)	179, 382
BsiHKCI	CYCGRG	1 cut(s)	191
BslFI	GGGAC	1 cut(s)	119
BslI	CCNNNNNNNGG	6 cut(s)	321, 764, 1967, 2024, 2396, 2570
BsmAI	GTCTC	4 cut(s)	684, 1182, 1532, 2485
BsmFI	GGGAC	1 cut(s)	119
BsnI	GGCC	8 cut(s)	76, 235, 275, 295, 568, 801, 1283, 1844
BsoBI	CYCGRG	1 cut(s)	191
Bsp1286I	GDGCHC	3 cut(s)	179, 382, 618
Bsp13I	TCCGGA	1 cut(s)	661
Bsp1407I	TGTACA	2 cut(s)	1715, 2062
Bsp143I	GATC	4 cut(s)	278, 1106, 1744, 1816
Bsp19I	CCATGG	1 cut(s)	162
BspANI	GGCC	8 cut(s)	76, 235, 275, 295, 568, 801, 1283, 1844
BspDI	ATCGAT	1 cut(s)	1082
BspEI	TCCGGA	1 cut(s)	661
BspFNI	CGCG	1 cut(s)	317
BspLI	GGNNCC	7 cut(s)	9, 399, 420, 567, 577, 615, 627
BspMAI	CTGCAG	3 cut(s)	24, 1531, 2590
BspMI	ACCTGC	3 cut(s)	1278, 2090, 2184
BspPI	GGATC	3 cut(s)	273, 1101, 1824
BspT107I	GGYRCC	2 cut(s)	397, 625
BsrBI	CCGCTC	1 cut(s)	252
BsrDI	GCAATG	4 cut(s)	567, 864, 2224, 2384
BsrFI	RCCGGY	3 cut(s)	426, 1397, 2241
BsrGI	TGTACA	2 cut(s)	1715, 2062
BsrI	ACTGG	4 cut(s)	898, 1036, 1354, 1987
BssAI	RCCGGY	3 cut(s)	426, 1397, 2241
BssMI	GATC	4 cut(s)	278, 1106, 1744, 1816
BssNI	GRCGYC	2 cut(s)	255, 398
BssT1I	CCWWGG	3 cut(s)	162, 1458, 1966
Bst2UI	CCWGG	3 cut(s)	610, 1974, 2019
Bst6I	CTCTTC	2 cut(s)	1202, 2122
BstACI	GRCGYC	2 cut(s)	255, 398
BstAPI	GCANNNNNTGC	1 cut(s)	859
BstAUI	TGTACA	2 cut(s)	1715, 2062
BstC8I	GCNNGC	6 cut(s)	382, 855, 1842, 2310, 2502, 2633
BstDSI	CCRYGG	2 cut(s)	162, 315
BstEII	GGTNACC	1 cut(s)	40
BstF5I	GGATG	7 cut(s)	45, 1218, 1627, 1945, 2197, 2212, 2554
BstFNI	CGCG	1 cut(s)	317
BstH2I	RGCGCY	1 cut(s)	401
BstHHI	GCGC	6 cut(s)	54, 400, 637, 853, 1380, 2631
BstKTI	GATC	4 cut(s)	281, 1109, 1747, 1819
BstMAI	GTCTC	4 cut(s)	684, 1182, 1532, 2485
BstMBI	GATC	4 cut(s)	278, 1106, 1744, 1816
BstMWI	GCNNNNNNNGC	7 cut(s)	98, 833, 859, 1719, 2019, 2096, 2386
BstNI	CCWGG	3 cut(s)	610, 1974, 2019
BstNSI	RCATGY	2 cut(s)	1722, 2312
BstPI	GGTNACC	1 cut(s)	40
BstSCI	CCNGG	4 cut(s)	608, 616, 1972, 2017
BstSFI	CTRYAG	4 cut(s)	20, 1527, 2451, 2586
BstUI	CGCG	1 cut(s)	317
BstV1I	GCAGC	6 cut(s)	34, 110, 544, 1387, 2138, 2572
BstV2I	GAAGAC	4 cut(s)	199, 457, 531, 1326
BstX2I	RGATCY	2 cut(s)	278, 1106
BstXI	CCANNNNNNTGG	1 cut(s)	1795
BstYI	RGATCY	2 cut(s)	278, 1106
Bsu15I	ATCGAT	1 cut(s)	1082
BsuRI	GGCC	8 cut(s)	76, 235, 275, 295, 568, 801, 1283, 1844
BsuTUI	ATCGAT	1 cut(s)	1082
BtgI	CCRYGG	2 cut(s)	162, 315
BtgZI	GCGATG	1 cut(s)	1932
BtrI	CACGTC	1 cut(s)	463
BtsCI	GGATG	7 cut(s)	45, 1218, 1627, 1945, 2197, 2212, 2554
BtsI	GCAGTG	4 cut(s)	32, 873, 1524, 2029
BveI	ACCTGC	3 cut(s)	1278, 2090, 2184
Cac8I	GCNNGC	6 cut(s)	382, 855, 1842, 2310, 2502, 2633
CfoI	GCGC	6 cut(s)	54, 400, 637, 853, 1380, 2631
Cfr10I	RCCGGY	3 cut(s)	426, 1397, 2241
Cfr13I	GGNCC	3 cut(s)	75, 566, 1970
Cfr42I	CCGCGG	1 cut(s)	318
ClaI	ATCGAT	1 cut(s)	1082
CseI	GACGC	3 cut(s)	244, 1886, 2301
Csp6I	GTAC	8 cut(s)	120, 626, 717, 1180, 1597, 1716, 2063, 2532
CspAI	ACCGGT	1 cut(s)	1397
CspCI	CAANNNNNGTGG	2 cut(s)	393, 428
CviAII	CATG	4 cut(s)	163, 1719, 2309, 2432
CviQI	GTAC	8 cut(s)	120, 626, 717, 1180, 1597, 1716, 2063, 2532
DinI	GGCGCC	1 cut(s)	399
DpnI	GATC	4 cut(s)	280, 1108, 1746, 1818
DpnII	GATC	4 cut(s)	278, 1106, 1744, 1816
DraI	TTTAAA	1 cut(s)	270
DriI	GACNNNNNGTC	1 cut(s)	2245
EaeI	YGGCCR	1 cut(s)	293
Eam1104I	CTCTTC	2 cut(s)	1202, 2122
Eam1105I	GACNNNNNGTC	1 cut(s)	2245
EarI	CTCTTC	2 cut(s)	1202, 2122
EciI	GGCGGA	2 cut(s)	1634, 2586
Ecl136II	GAGCTC	1 cut(s)	177
Eco130I	CCWWGG	3 cut(s)	162, 1458, 1966
Eco147I	AGGCCT	3 cut(s)	801, 1283, 1844
Eco24I	GRGCYC	2 cut(s)	179, 618
Eco47I	GGWCC	1 cut(s)	1970
Eco53kI	GAGCTC	1 cut(s)	177
Eco57I	CTGAAG	3 cut(s)	1338, 1715, 2621
Eco88I	CYCGRG	1 cut(s)	191
Eco91I	GGTNACC	1 cut(s)	40
EcoICRI	GAGCTC	1 cut(s)	177
EcoO109I	RGGNCCY	1 cut(s)	566
EcoO65I	GGTNACC	1 cut(s)	40
EcoRI	GAATTC	1 cut(s)	216
EcoRII	CCWGG	3 cut(s)	608, 1972, 2017
EcoT14I	CCWWGG	3 cut(s)	162, 1458, 1966
EcoT38I	GRGCYC	2 cut(s)	179, 618
EgeI	GGCGCC	1 cut(s)	399
EheI	GGCGCC	1 cut(s)	399
ErhI	CCWWGG	3 cut(s)	162, 1458, 1966
FaeI	CATG	4 cut(s)	166, 1722, 2312, 2435
FaqI	GGGAC	1 cut(s)	119
FatI	CATG	4 cut(s)	162, 1718, 2308, 2431
FauI	CCCGC	1 cut(s)	2509
FauNDI	CATATG	1 cut(s)	2280
FbaI	TGATCA	1 cut(s)	1744
FblI	GTMKAC	1 cut(s)	444
FokI	GGATG	7 cut(s)	32, 1225, 1634, 1952, 2204, 2219, 2561
FriOI	GRGCYC	2 cut(s)	179, 618
FspBI	CTAG	4 cut(s)	570, 1365, 1886, 2250
FspI	TGCGCA	1 cut(s)	852
GlaI	GCGC	6 cut(s)	53, 399, 636, 852, 1379, 2630
GsuI	CTGGAG	2 cut(s)	1029, 2495
HaeII	RGCGCY	1 cut(s)	401
HaeIII	GGCC	8 cut(s)	76, 235, 275, 295, 568, 801, 1283, 1844
HgaI	GACGC	3 cut(s)	244, 1886, 2301
HhaI	GCGC	6 cut(s)	54, 400, 637, 853, 1380, 2631
Hin1I	GRCGYC	2 cut(s)	255, 398
Hin1II	CATG	4 cut(s)	166, 1722, 2312, 2435
Hin6I	GCGC	6 cut(s)	52, 398, 635, 851, 1378, 2629
HinP1I	GCGC	6 cut(s)	52, 398, 635, 851, 1378, 2629
HincII	GTYRAC	5 cut(s)	1000, 1173, 1522, 1858, 2239
HindII	GTYRAC	5 cut(s)	1000, 1173, 1522, 1858, 2239
HphI	GGTGA	9 cut(s)	34, 53, 382, 400, 520, 779, 1259, 1278, 1958
Hpy188III	TCNNGA	9 cut(s)	142, 485, 532, 662, 1239, 1256, 1365, 1676, 2250
Hpy99I	CGWCG	5 cut(s)	148, 241, 260, 263, 2206
HpyAV	CCTTC	8 cut(s)	529, 1193, 1335, 1585, 1690, 2386, 2390, 2585
HpyCH4IV	ACGT	3 cut(s)	288, 462, 868
HpyF10VI	GCNNNNNNNGC	7 cut(s)	98, 833, 859, 1719, 2019, 2096, 2386
HpySE526I	ACGT	3 cut(s)	288, 462, 868
Hsp92I	GRCGYC	2 cut(s)	255, 398
Hsp92II	CATG	4 cut(s)	166, 1722, 2312, 2435
HspAI	GCGC	6 cut(s)	52, 398, 635, 851, 1378, 2629
KasI	GGCGCC	1 cut(s)	397
Kpn2I	TCCGGA	1 cut(s)	661
KpnI	GGTACC	1 cut(s)	629
Ksp22I	TGATCA	1 cut(s)	1744
KspI	CCGCGG	1 cut(s)	318
Kzo9I	GATC	4 cut(s)	278, 1106, 1744, 1816
LmnI	GCTCC	8 cut(s)	7, 249, 581, 613, 1010, 1043, 1117, 2164
Lsp1109I	GCAGC	6 cut(s)	34, 110, 544, 1387, 2138, 2572
LweI	GCATC	5 cut(s)	814, 1129, 1645, 1909, 2625
MaeI	CTAG	4 cut(s)	570, 1365, 1886, 2250
MaeII	ACGT	3 cut(s)	288, 462, 868
MaeIII	GTNAC	4 cut(s)	40, 388, 1215, 1769
MalI	GATC	4 cut(s)	280, 1108, 1746, 1818
MbiI	CCGCTC	1 cut(s)	252
MboI	GATC	4 cut(s)	278, 1106, 1744, 1816
MflI	RGATCY	2 cut(s)	278, 1106
MhlI	GDGCHC	3 cut(s)	179, 382, 618
Mly113I	GGCGCC	1 cut(s)	398
MlyI	GAGTC	6 cut(s)	213, 580, 686, 1415, 2060, 2366
MmeI	TCCRAC	3 cut(s)	219, 241, 2184
MroI	TCCGGA	1 cut(s)	661
MroXI	GAANNNNTTC	1 cut(s)	153
MseI	TTAA	7 cut(s)	269, 924, 1226, 1308, 1394, 1493, 2340
MslI	CAYNNNNRTG	2 cut(s)	701, 1082
MspA1I	CMGCKG	1 cut(s)	317
MspR9I	CCNGG	4 cut(s)	610, 618, 1974, 2019
MvaI	CCWGG	3 cut(s)	610, 1974, 2019
MvnI	CGCG	1 cut(s)	317
MwoI	GCNNNNNNNGC	7 cut(s)	98, 833, 859, 1719, 2019, 2096, 2386
NarI	GGCGCC	1 cut(s)	398
NciI	CCSGG	1 cut(s)	618
NcoI	CCATGG	1 cut(s)	162
NdeI	CATATG	1 cut(s)	2280
NdeII	GATC	4 cut(s)	278, 1106, 1744, 1816
NlaIII	CATG	4 cut(s)	166, 1722, 2312, 2435
NlaIV	GGNNCC	7 cut(s)	9, 399, 420, 567, 577, 615, 627
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	4 cut(s)	40, 388, 1215, 1769
NsbI	TGCGCA	1 cut(s)	852
NspI	RCATGY	2 cut(s)	1722, 2312
PaeI	GCATGC	1 cut(s)	2312
PaqCI	CACCTGC	1 cut(s)	1278
PasI	CCCWGGG	1 cut(s)	609
PceI	AGGCCT	3 cut(s)	801, 1283, 1844
PdmI	GAANNNNTTC	1 cut(s)	153
PinAI	ACCGGT	1 cut(s)	1397
PleI	GAGTC	6 cut(s)	212, 580, 685, 1414, 2059, 2365
PluTI	GGCGCC	1 cut(s)	401
PpsI	GAGTC	6 cut(s)	212, 580, 685, 1414, 2059, 2365
Psp124BI	GAGCTC	1 cut(s)	179
Psp1406I	AACGTT	1 cut(s)	288
Psp6I	CCWGG	3 cut(s)	608, 1972, 2017
PspEI	GGTNACC	1 cut(s)	40
PspGI	CCWGG	3 cut(s)	608, 1972, 2017
PspN4I	GGNNCC	7 cut(s)	9, 399, 420, 567, 577, 615, 627
PspPI	GGNCC	3 cut(s)	75, 566, 1970
PstI	CTGCAG	3 cut(s)	24, 1531, 2590
PsuI	RGATCY	2 cut(s)	278, 1106
RsaI	GTAC	8 cut(s)	121, 627, 718, 1181, 1598, 1717, 2064, 2533
RsaNI	GTAC	8 cut(s)	120, 626, 717, 1180, 1597, 1716, 2063, 2532
RseI	CAYNNNNRTG	2 cut(s)	701, 1082
SacI	GAGCTC	1 cut(s)	179
SacII	CCGCGG	1 cut(s)	318
SaqAI	TTAA	7 cut(s)	269, 924, 1226, 1308, 1394, 1493, 2340
Sau3AI	GATC	4 cut(s)	278, 1106, 1744, 1816
Sau96I	GGNCC	3 cut(s)	75, 566, 1970
ScaI	AGTACT	3 cut(s)	718, 1181, 2533
SchI	GAGTC	6 cut(s)	213, 580, 686, 1415, 2060, 2366
ScrFI	CCNGG	4 cut(s)	610, 618, 1974, 2019
SduI	GDGCHC	3 cut(s)	179, 382, 618
SfaNI	GCATC	5 cut(s)	814, 1129, 1645, 1909, 2625
SfcI	CTRYAG	4 cut(s)	20, 1527, 2451, 2586
SfoI	GGCGCC	1 cut(s)	399
Sfr303I	CCGCGG	1 cut(s)	318
SgrBI	CCGCGG	1 cut(s)	318
SinI	GGWCC	1 cut(s)	1970
SmiMI	CAYNNNNRTG	2 cut(s)	701, 1082
SmlI	CTYRAG	4 cut(s)	431, 1102, 1183, 1676
SmoI	CTYRAG	4 cut(s)	431, 1102, 1183, 1676
SphI	GCATGC	1 cut(s)	2312
SseBI	AGGCCT	3 cut(s)	801, 1283, 1844
SspDI	GGCGCC	1 cut(s)	397
SspI	AATATT	2 cut(s)	1333, 1825
SspMI	CTAG	4 cut(s)	570, 1365, 1886, 2250
SstI	GAGCTC	1 cut(s)	179
StuI	AGGCCT	3 cut(s)	801, 1283, 1844
StyD4I	CCNGG	4 cut(s)	608, 616, 1972, 2017
StyI	CCWWGG	3 cut(s)	162, 1458, 1966
TaiI	ACGT	3 cut(s)	291, 465, 871
TaqI	TCGA	6 cut(s)	173, 179, 220, 829, 931, 1082
TatI	WGTACW	7 cut(s)	119, 716, 1179, 1596, 1715, 2062, 2531
TauI	GCSGC	7 cut(s)	104, 255, 298, 317, 320, 403, 1469
Tru1I	TTAA	7 cut(s)	269, 924, 1226, 1308, 1394, 1493, 2340
Tru9I	TTAA	7 cut(s)	269, 924, 1226, 1308, 1394, 1493, 2340
TseFI	GTSAC	4 cut(s)	40, 388, 1215, 1769
TseI	GCWGC	6 cut(s)	22, 98, 557, 1375, 2151, 2585
Tsp45I	GTSAC	4 cut(s)	40, 388, 1215, 1769
TspDTI	ATGAA	8 cut(s)	685, 993, 1057, 1403, 1641, 1878, 1920, 2420
TspGWI	ACGGA	2 cut(s)	161, 2639
VpaK11BI	GGWCC	1 cut(s)	1970
XapI	RAATTY	4 cut(s)	153, 216, 886, 2524
XbaI	TCTAGA	2 cut(s)	1364, 2249
XceI	RCATGY	2 cut(s)	1722, 2312
XmiI	GTMKAC	1 cut(s)	444
XmnI	GAANNNNTTC	1 cut(s)	153
XspI	CTAG	4 cut(s)	570, 1365, 1886, 2250
ZrmI	AGTACT	3 cut(s)	718, 1181, 2533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.