MD08G1032700.v1.1

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008377
Physical Location & Seq
Reverse (-)
2323793 .. 2327351
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1032700.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGCCATCTTCTTACTTGTCGAAGATTAGGACTGCGTTGCAGAGGTCGATTGTTGGGCAGGAAGGAGCGCTTCAGGAATCAGTAGATGGGTTAATTGGGCAGGGAAAATTGTTGTTTGGAAATTCTAAGTTGTTCCAGTCTCCGTTTTCAACTATTTCAGGCCTCCAAGCATTGTTTTCACCAGGCATTGTTTTTGCTGCGAGGTTGGATTCGCAGCTAGTTAACAAAAGAAGGAACATTTCTGTTGTTGAAGAGATTTCGCGCATTATTTCAACCCCATCTGTGTCAGGGCCTTCAATTCAGGTTTGCGGGTATCATATCGATTGTGTGCTTTCTGAGCCCAGTCAGTTTGTGACCAGAAGTAAGTTTCAGAATAAACCTATGGCTGCTTGTGGTTCTAGAACTTTATTTGGGGGATTCTGCCCAGATAATTTAACTTCAAGGCCTGGGCTTCATGCAATGGTACCCAAAAGTGCCTGTACATTATACAACAAAAGAAGTTCGGACTGTTTTCAAACAGCTAGCATGAGTTTGAATAAAAGTGGGCAGTCCAACACTAATGTAATTCATGGGTATTTGATTTATGAAGTTGGAAAGAGATGGTGTAATTCCTCCCCAAGTGAAGGATCAGGGTCAAGAGAATTTCATGGTTCATCAACATGCTCATCTACAAGGACTGCCCATGACGTGTCTTTCGATAGTTCTGCTTCTGAAGGGCAAGTTTCAAGTTCTGCAGATTCATCTAATGAGAAGATTACAGATGGAAAGTCCCTAAAACTAACTTCAGGATCATATTACCTGCCCCATCCAGATAAAGAGGAAACTGGCGGGGAGGATGCTCACTTCATTTGTGCAAATGAACAAGCAATAGGGGTGGCGGATGGTGTTGGTGGCTGGGCAGATCTCGGTGTTAATTCCGGATTGTACTCCAGAGAACTGATGTCTAATTCTGTGGCCGCTGTTCAGGAGGAGCCCAAGGGTGCCATTGACCCTGCTCGGGTATTGGAGAAAGCTCACTCGAGCACGAAAGCCAGGGGTTCCTCAACAGCATGCATCATAGCACTCACAGAACAGGGTATTCATGCAGTTAATTTAGGTGATAGTGGGTTTATAGTGGTTCGAGGCGGGTCCACCGTCTTTAGATCTCCTGTGCAACAGCATAATTTTAACTTTTCCTATCAACTGGGGACTGGAAACAACGGTGATCTACCTAGCTCTGGCCAGGTTTTTACTGTCCCTGTTGCACCCAGGGATGTTATAATTGCCGGAACTGATGGCCTGTTCGACAACTTGTACAACAACGAGATTACTGCGGTAGTGGTCCATGCCATGAGAGCTGGCTTAGGGCCTCAGGTGACAGCGCAGAAGATTGCAGCGTTGGCACGCCAACGAGCACAGGATCGAAACCGGCAGACGCCTTTCTCCACCGCCGCTCAAGATGCTGGGGTCCTTCATTATGGCGGCAAGCTTGATGATATAACTGTTGTTGTTTCATATGTGACCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C_2 SpoIIE

Protein Analysis

503

Amino Acids

53.24

Weight (kDa)

7.17

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 336 - 451 3e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1259
Acc36I ACCTGC 1 cut(s) 807
Acc65I GGTACC 1 cut(s) 463
AccB1I GGYRCC 2 cut(s) 463, 980
AccBSI CCGCTC 1 cut(s) 1433
AccII CGCG 1 cut(s) 262
AccIII TCCGGA 1 cut(s) 917
AciI CCGC 9 cut(s) 309, 828, 878, 957, 1125, 1313, 1428, 1431, 1461
AclWI GGATC 3 cut(s) 634, 796, 1407
AcoI YGGCCR 2 cut(s) 954, 1219
AcsI RAATTY 2 cut(s) 121, 641
AcuI CTGAAG 3 cut(s) 56, 732, 768
AcyI GRCGYC 1 cut(s) 1415
AfaI GTAC 4 cut(s) 465, 481, 926, 1295
AfeI AGCGCT 1 cut(s) 69
AfiI CCNNNNNNNGG 3 cut(s) 623, 997, 1217
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 8 cut(s) 150, 251, 273, 297, 441, 515, 535, 726
AjiI CACGTC 1 cut(s) 688
AjnI CCWGG 5 cut(s) 181, 445, 1031, 1221, 1247
AluBI AGCT 6 cut(s) 217, 521, 1013, 1215, 1337, 1468
AluI AGCT 6 cut(s) 217, 521, 1013, 1215, 1337, 1468
Alw21I GWGCWC 2 cut(s) 1025, 1396
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 3 cut(s) 634, 796, 1407
Ama87I CYCGRG 2 cut(s) 996, 1018
Aor13HI TCCGGA 1 cut(s) 917
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 7 cut(s) 160, 290, 443, 954, 1219, 1276, 1346
ApeKI GCWGC 4 cut(s) 197, 214, 386, 1373
ApoI RAATTY 2 cut(s) 121, 641
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp718I GGTACC 1 cut(s) 463
AspLEI GCGC 3 cut(s) 70, 264, 1363
AspS9I GGNCC 5 cut(s) 290, 1128, 1321, 1346, 1447
AsuHPI GGTGA 4 cut(s) 171, 1109, 1214, 1366
AsuNHI GCTAGC 1 cut(s) 521
AvaI CYCGRG 2 cut(s) 996, 1018
AvaII GGWCC 3 cut(s) 1128, 1321, 1447
AxyI CCTNAGG 1 cut(s) 1350
BalI TGGCCA 1 cut(s) 1221
BanI GGYRCC 2 cut(s) 463, 980
BanII GRGCYC 2 cut(s) 342, 975
Bbv12I GWGCWC 2 cut(s) 1025, 1396
BbvI GCAGC 4 cut(s) 184, 226, 373, 1385
BccI CCATC 8 cut(s) 13, 80, 286, 594, 755, 813, 875, 1268
BcgI CGANNNNNNTGC 4 cut(s) 686, 720, 1292, 1326
BciT130I CCWGG 5 cut(s) 183, 447, 1033, 1223, 1249
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 4 cut(s) 218, 399, 522, 1212
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 1 cut(s) 71
BfuAI ACCTGC 1 cut(s) 807
BglII AGATCT 2 cut(s) 901, 1142
BisI GCNGC 7 cut(s) 198, 215, 387, 957, 1374, 1431, 1462
BlsI GCNGC 7 cut(s) 199, 216, 388, 958, 1375, 1432, 1463
Bme1390I CCNGG 5 cut(s) 183, 447, 1033, 1223, 1249
Bme18I GGWCC 3 cut(s) 1128, 1321, 1447
BmeT110I CYCGRG 2 cut(s) 996, 1018
BmgBI CACGTC 1 cut(s) 688
BmgT120I GGNCC 5 cut(s) 290, 1128, 1321, 1346, 1447
BmiI GGNNCC 6 cut(s) 465, 972, 982, 1039, 1129, 1448
BmrFI CCNGG 5 cut(s) 183, 447, 1033, 1223, 1249
BmrI ACTGGG 2 cut(s) 336, 1193
BmsI GCATC 3 cut(s) 826, 1062, 1429
BmtI GCTAGC 1 cut(s) 525
BmuI ACTGGG 2 cut(s) 336, 1193
BpmI CTGGAG 1 cut(s) 913
Bpu10I CCTNAGC 1 cut(s) 1342
BpuEI CTTGAG 1 cut(s) 1419
Bsa29I ATCGAT 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 1415
BsaJI CCNNGG 5 cut(s) 446, 975, 1032, 1247, 1248
BsaWI WCCGGW 1 cut(s) 917
Bsc4I CCNNNNNNNGG 3 cut(s) 623, 997, 1217
Bse118I RCCGGY 1 cut(s) 1407
Bse1I ACTGG 6 cut(s) 136, 342, 829, 1188, 1195, 1503
Bse21I CCTNAGG 1 cut(s) 1350
Bse3DI GCAATG 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 917
BseBI CCWGG 5 cut(s) 183, 447, 1033, 1223, 1249
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 5 cut(s) 446, 975, 1032, 1247, 1248
BseGI GGATG 4 cut(s) 805, 841, 886, 1258
BseLI CCNNNNNNNGG 3 cut(s) 623, 997, 1217
BseMI GCAATG 1 cut(s) 465
BseMII CTCAG 2 cut(s) 327, 1364
BseNI ACTGG 6 cut(s) 136, 342, 829, 1188, 1195, 1503
BseRI GAGGAG 1 cut(s) 983
BseXI GCAGC 4 cut(s) 184, 226, 373, 1385
BseYI CCCAGC 2 cut(s) 894, 1442
Bsh1236I CGCG 1 cut(s) 262
BshFI GGCC 7 cut(s) 162, 292, 445, 956, 1221, 1278, 1348
BshNI GGYRCC 2 cut(s) 463, 980
BshVI ATCGAT 1 cut(s) 321
BsiHKAI GWGCWC 2 cut(s) 1025, 1396
BsiHKCI CYCGRG 2 cut(s) 996, 1018
BsiSI CCGG 3 cut(s) 918, 1266, 1408
BslFI GGGAC 3 cut(s) 754, 1201, 1220
BslI CCNNNNNNNGG 3 cut(s) 623, 997, 1217
BsmAI GTCTC 1 cut(s) 144
BsmFI GGGAC 3 cut(s) 754, 1201, 1220
BsnI GGCC 7 cut(s) 162, 292, 445, 956, 1221, 1278, 1348
BsoBI CYCGRG 2 cut(s) 996, 1018
Bsp1286I GDGCHC 4 cut(s) 342, 975, 1025, 1396
Bsp13I TCCGGA 1 cut(s) 917
Bsp1407I TGTACA 2 cut(s) 479, 1293
Bsp143I GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
BspACI CCGC 9 cut(s) 309, 828, 878, 957, 1125, 1313, 1428, 1431, 1461
BspANI GGCC 7 cut(s) 162, 292, 445, 956, 1221, 1278, 1348
BspCNI CTCAG 2 cut(s) 328, 1363
BspDI ATCGAT 1 cut(s) 321
BspEI TCCGGA 1 cut(s) 917
BspFNI CGCG 1 cut(s) 262
BspLI GGNNCC 6 cut(s) 465, 972, 982, 1039, 1129, 1448
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 1 cut(s) 807
BspOI GCTAGC 1 cut(s) 525
BspPI GGATC 3 cut(s) 634, 796, 1407
BspT107I GGYRCC 2 cut(s) 463, 980
BsrBI CCGCTC 1 cut(s) 1433
BsrDI GCAATG 1 cut(s) 465
BsrFI RCCGGY 1 cut(s) 1407
BsrGI TGTACA 2 cut(s) 479, 1293
BsrI ACTGG 6 cut(s) 136, 342, 829, 1188, 1195, 1503
BssAI RCCGGY 1 cut(s) 1407
BssECI CCNNGG 5 cut(s) 446, 975, 1032, 1247, 1248
BssMI GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
BssNI GRCGYC 1 cut(s) 1415
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 5 cut(s) 183, 447, 1033, 1223, 1249
Bst4CI ACNGT 5 cut(s) 509, 1135, 1202, 1234, 1483
Bst6I CTCTTC 1 cut(s) 246
BstACI GRCGYC 1 cut(s) 1415
BstAUI TGTACA 2 cut(s) 479, 1293
BstC8I GCNNGC 5 cut(s) 523, 1051, 1339, 1384, 1466
BstDEI CTNAG 4 cut(s) 126, 336, 1342, 1350
BstF5I GGATG 4 cut(s) 805, 841, 886, 1258
BstFNI CGCG 1 cut(s) 262
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 3 cut(s) 70, 264, 1363
BstKTI GATC 6 cut(s) 629, 791, 904, 1145, 1207, 1402
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
BstMWI GCNNNNNNNGC 4 cut(s) 337, 1334, 1379, 1439
BstNI CCWGG 5 cut(s) 183, 447, 1033, 1223, 1249
BstNSI RCATGY 2 cut(s) 663, 1053
BstSCI CCNGG 5 cut(s) 181, 445, 1031, 1221, 1247
BstSFI CTRYAG 1 cut(s) 732
BstUI CGCG 1 cut(s) 262
BstV1I GCAGC 4 cut(s) 184, 226, 373, 1385
BstX2I RGATCY 2 cut(s) 901, 1142
BstYI RGATCY 2 cut(s) 901, 1142
Bsu15I ATCGAT 1 cut(s) 321
Bsu36I CCTNAGG 1 cut(s) 1350
BsuRI GGCC 7 cut(s) 162, 292, 445, 956, 1221, 1278, 1348
BsuTUI ATCGAT 1 cut(s) 321
BtrI CACGTC 1 cut(s) 688
BtsCI GGATG 4 cut(s) 805, 841, 886, 1258
BveI ACCTGC 1 cut(s) 807
Cac8I GCNNGC 5 cut(s) 523, 1051, 1339, 1384, 1466
CfoI GCGC 3 cut(s) 70, 264, 1363
Cfr10I RCCGGY 1 cut(s) 1407
Cfr13I GGNCC 5 cut(s) 290, 1128, 1321, 1346, 1447
ClaI ATCGAT 1 cut(s) 321
CseI GACGC 1 cut(s) 1423
Csp6I GTAC 4 cut(s) 464, 480, 925, 1294
CspCI CAANNNNNGTGG 2 cut(s) 855, 890
CviQI GTAC 4 cut(s) 464, 480, 925, 1294
DdeI CTNAG 4 cut(s) 126, 336, 1342, 1350
DpnI GATC 6 cut(s) 628, 790, 903, 1144, 1206, 1401
DpnII GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
EaeI YGGCCR 2 cut(s) 954, 1219
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
EciI GGCGGA 1 cut(s) 893
Eco130I CCWWGG 1 cut(s) 975
Eco147I AGGCCT 2 cut(s) 162, 445
Eco24I GRGCYC 2 cut(s) 342, 975
Eco47I GGWCC 3 cut(s) 1128, 1321, 1447
Eco47III AGCGCT 1 cut(s) 69
Eco57I CTGAAG 3 cut(s) 56, 732, 768
Eco81I CCTNAGG 1 cut(s) 1350
Eco88I CYCGRG 2 cut(s) 996, 1018
EcoO109I RGGNCCY 3 cut(s) 290, 1346, 1447
EcoRII CCWGG 5 cut(s) 181, 445, 1031, 1221, 1247
EcoT14I CCWWGG 1 cut(s) 975
EcoT22I ATGCAT 1 cut(s) 1055
EcoT38I GRGCYC 2 cut(s) 342, 975
ErhI CCWWGG 1 cut(s) 975
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FaqI GGGAC 3 cut(s) 754, 1201, 1220
FauI CCCGC 3 cut(s) 302, 821, 1118
FauNDI CATATG 1 cut(s) 1495
Fnu4HI GCNGC 7 cut(s) 198, 215, 387, 957, 1374, 1431, 1462
FokI GGATG 4 cut(s) 792, 848, 893, 1265
FriOI GRGCYC 2 cut(s) 342, 975
Fsp4HI GCNGC 7 cut(s) 198, 215, 387, 957, 1374, 1431, 1462
FspBI CTAG 4 cut(s) 218, 399, 522, 1212
GlaI GCGC 3 cut(s) 69, 263, 1362
GluI GCNGC 7 cut(s) 198, 215, 387, 957, 1374, 1431, 1462
GsaI CCCAGC 2 cut(s) 898, 1446
GsuI CTGGAG 1 cut(s) 913
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 7 cut(s) 162, 292, 445, 956, 1221, 1278, 1348
HapII CCGG 3 cut(s) 918, 1266, 1408
HgaI GACGC 1 cut(s) 1423
HhaI GCGC 3 cut(s) 70, 264, 1363
Hin1I GRCGYC 1 cut(s) 1415
Hin6I GCGC 3 cut(s) 68, 262, 1361
HinP1I GCGC 3 cut(s) 68, 262, 1361
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 1466
HinfI GANTC 4 cut(s) 77, 209, 417, 737
HpaI GTTAAC 1 cut(s) 223
HpaII CCGG 3 cut(s) 918, 1266, 1408
HphI GGTGA 4 cut(s) 171, 1109, 1214, 1366
Hpy166II GTNNAC 2 cut(s) 223, 1131
Hpy188I TCNGA 4 cut(s) 337, 372, 505, 712
Hpy188III TCNNGA 9 cut(s) 74, 399, 636, 786, 809, 918, 930, 965, 1436
Hpy8I GTNNAC 2 cut(s) 223, 1131
HpyAV CCTTC 6 cut(s) 56, 225, 303, 617, 707, 1460
HpyCH4III ACNGT 5 cut(s) 509, 1135, 1202, 1234, 1483
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 9 cut(s) 40, 458, 734, 854, 1053, 1085, 1153, 1244, 1373
HpyF10VI GCNNNNNNNGC 4 cut(s) 337, 1334, 1379, 1439
HpyF3I CTNAG 4 cut(s) 126, 336, 1342, 1350
HpySE526I ACGT 1 cut(s) 687
Hsp92I GRCGYC 1 cut(s) 1415
HspAI GCGC 3 cut(s) 68, 262, 1361
Kpn2I TCCGGA 1 cut(s) 917
KpnI GGTACC 1 cut(s) 467
KspAI GTTAAC 1 cut(s) 223
Kzo9I GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
LmnI GCTCC 2 cut(s) 65, 970
Lsp1109I GCAGC 4 cut(s) 184, 226, 373, 1385
LweI GCATC 3 cut(s) 826, 1062, 1429
MaeI CTAG 4 cut(s) 218, 399, 522, 1212
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 3 cut(s) 352, 1354, 1498
MalI GATC 6 cut(s) 628, 790, 903, 1144, 1206, 1401
MbiI CCGCTC 1 cut(s) 1433
MboI GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
MboII GAAGA 4 cut(s) 34, 263, 763, 1378
MflI RGATCY 2 cut(s) 901, 1142
MhlI GDGCHC 4 cut(s) 342, 975, 1025, 1396
MlsI TGGCCA 1 cut(s) 1221
MluNI TGGCCA 1 cut(s) 1221
MmeI TCCRAC 3 cut(s) 186, 571, 576
Mox20I TGGCCA 1 cut(s) 1221
Mph1103I ATGCAT 1 cut(s) 1055
MroI TCCGGA 1 cut(s) 917
MscI TGGCCA 1 cut(s) 1221
MseI TTAA 6 cut(s) 92, 222, 434, 912, 1089, 1167
MslI CAYNNNNRTG 1 cut(s) 658
Msp20I TGGCCA 1 cut(s) 1221
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 3 cut(s) 918, 1266, 1408
MspR9I CCNGG 5 cut(s) 183, 447, 1033, 1223, 1249
MvaI CCWGG 5 cut(s) 183, 447, 1033, 1223, 1249
MvnI CGCG 1 cut(s) 262
MwoI GCNNNNNNNGC 4 cut(s) 337, 1334, 1379, 1439
NdeI CATATG 1 cut(s) 1495
NdeII GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
NheI GCTAGC 1 cut(s) 521
NlaIV GGNNCC 6 cut(s) 465, 972, 982, 1039, 1129, 1448
NmuCI GTSAC 3 cut(s) 352, 1354, 1498
NsiI ATGCAT 1 cut(s) 1055
NspI RCATGY 2 cut(s) 663, 1053
PaeI GCATGC 1 cut(s) 1053
PaeR7I CTCGAG 1 cut(s) 1018
PasI CCCWGGG 1 cut(s) 1248
PceI AGGCCT 2 cut(s) 162, 445
PcsI WCGNNNNNNNCGW 1 cut(s) 693
PfeI GAWTC 4 cut(s) 77, 209, 417, 737
PkrI GCNGC 7 cut(s) 199, 216, 388, 958, 1375, 1432, 1463
PpuMI RGGWCCY 1 cut(s) 1447
PsiI TTATAA 1 cut(s) 1259
Psp5II RGGWCCY 1 cut(s) 1447
Psp6I CCWGG 5 cut(s) 181, 445, 1031, 1221, 1247
PspFI CCCAGC 2 cut(s) 894, 1442
PspGI CCWGG 5 cut(s) 181, 445, 1031, 1221, 1247
PspN4I GGNNCC 6 cut(s) 465, 972, 982, 1039, 1129, 1448
PspPI GGNCC 5 cut(s) 290, 1128, 1321, 1346, 1447
PspPPI RGGWCCY 1 cut(s) 1447
PspXI VCTCGAGB 1 cut(s) 1018
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 2 cut(s) 901, 1142
RsaI GTAC 4 cut(s) 465, 481, 926, 1295
RsaNI GTAC 4 cut(s) 464, 480, 925, 1294
RseI CAYNNNNRTG 1 cut(s) 658
SaqAI TTAA 6 cut(s) 92, 222, 434, 912, 1089, 1167
SatI GCNGC 7 cut(s) 198, 215, 387, 957, 1374, 1431, 1462
Sau3AI GATC 6 cut(s) 626, 788, 901, 1142, 1204, 1399
Sau96I GGNCC 5 cut(s) 290, 1128, 1321, 1346, 1447
ScrFI CCNGG 5 cut(s) 183, 447, 1033, 1223, 1249
SduI GDGCHC 4 cut(s) 342, 975, 1025, 1396
SfaNI GCATC 3 cut(s) 826, 1062, 1429
SfcI CTRYAG 1 cut(s) 732
Sfr274I CTCGAG 1 cut(s) 1018
SinI GGWCC 3 cut(s) 1128, 1321, 1447
SlaI CTCGAG 1 cut(s) 1018
SmiMI CAYNNNNRTG 1 cut(s) 658
SmlI CTYRAG 2 cut(s) 1018, 1434
SmoI CTYRAG 2 cut(s) 1018, 1434
SphI GCATGC 1 cut(s) 1053
SseBI AGGCCT 2 cut(s) 162, 445
SsiI CCGC 9 cut(s) 309, 828, 878, 957, 1125, 1313, 1428, 1431, 1461
SspMI CTAG 4 cut(s) 218, 399, 522, 1212
StuI AGGCCT 2 cut(s) 162, 445
StyD4I CCNGG 5 cut(s) 181, 445, 1031, 1221, 1247
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 5 cut(s) 509, 1135, 1202, 1234, 1483
TaiI ACGT 1 cut(s) 690
TaqI TCGA 8 cut(s) 20, 47, 321, 696, 1019, 1120, 1284, 1402
TatI WGTACW 3 cut(s) 479, 924, 1293
TauI GCSGC 3 cut(s) 959, 1433, 1464
TfiI GAWTC 4 cut(s) 77, 209, 417, 737
Tru1I TTAA 6 cut(s) 92, 222, 434, 912, 1089, 1167
Tru9I TTAA 6 cut(s) 92, 222, 434, 912, 1089, 1167
TseFI GTSAC 3 cut(s) 352, 1354, 1498
TseI GCWGC 4 cut(s) 197, 214, 386, 1373
Tsp45I GTSAC 3 cut(s) 352, 1354, 1498
TspGWI ACGGA 1 cut(s) 132
VpaK11BI GGWCC 3 cut(s) 1128, 1321, 1447
XapI RAATTY 2 cut(s) 121, 641
XbaI TCTAGA 1 cut(s) 398
XceI RCATGY 2 cut(s) 663, 1053
XhoI CTCGAG 1 cut(s) 1018
XspI CTAG 4 cut(s) 218, 399, 522, 1212
Zsp2I ATGCAT 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.