RLG00000011312

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008377
Physical Location & Seq
Forward (+)
8484036 .. 8486512
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011312

Sequence Viewer

Length: 1518 bp
ATGCCATCTTCTTATTTATCCAAGCTTAGGACTGCAGTTCAGAGATCGATTGTAGGCCAGGAAGGTGTTGTTCACGACTCGCTAGAGGGGCTAATTGGGCAAGGCAAATTGTTGTTTGGAAATTCTAGGCTGTTCCATTCGAGACCCTTTTCGGCTATTTCAGACCTGAATGCATTTCTGCCGCCGGGCACTGTTGTCGCTGCTGCAAGGTCGGATTCGCAGTTAGTTAACCAGAGGAAGCAGATTTCTGTTGTTGGGGAGATTTCGCGGATCATTTCAACTCCATCTGTGTCAGGGCCTTCGTTCCAAGTTTGTGGGTATCATATTGATTCTGTGCTTTCGGATCCCAGTCAGTTTCAGACTAAAGCCATGGCTTTGTGCGGTTCTAGAGCTTTAGTTGGGGCGTGTTCCCCCCCGGATACTTTAACATCAAGGCGTGGGCTGCTTTCATTGTTACCTGAAAGTGCTTGTACATTTTATAATAACAGAAGTAAAAGATCTGATGGTTGCCAGAAAGCAAGCATGAGTTTGAAGAACAAAGGGCCTCCCAACACTAATGCAACTTATGGATATTTCGTATATGAAGTTGGGAAGAGATGGTGTAGTAACTTCCCAATAAATGGATTAGGCGCGAGAGAATTTCATAATTCTTCCACACGTTTCTCTGCTGGGACCGCCCATGATGTGTCTTTTGATAATTCTGCTCCTGAGGAGCAGCTTTCAAGTTCTACAGATTCATCGGATCAGAAGAACACAGATGCAAAGTCCTTAAAACTAAGTTCAGGATCATGCTACCTGCCCCATCCTGATAAAGAGGACACTGGCGGGGAGGATGCTCACTTCATATGTGTGAATGAACAAGCAATAGGGGTGGCAGATGGTGTTGGTGGCTGGGCAGATCATGGTGTTAACTCAGGATTGTATTCCCGAGAACTGATGTCTAATTCTGTAACAGCTGTTCAGGAGGAACCCAAAGGTTCAGTTGATCCTGCTAGGGTGTTGGAGAAAGCTCACTCCAGCACAAAAGCCAGAGGTTCCTCAACAGCATGCATCATAGCACTCACAGAACAGGGTATACATGCAATTAATTTAGGTGATAGTGGGTTTATAGTTGTTCGAGACGGGTGCACCGTCTTCAGATCCCCTGTGCAGCAGCATGATTTCAACTTCACTTATCAACTGGAGAATGGAGTCAACAGTGATCTACCTAGCTCTGGCCAGGTTTTTACAGTTCCTGTTGCTCCTGGGGATGTTATAGTTGCTGGAACTGATGGCCTGTTTGACAACTTGTATAACAATGAGATTACTGCAGTTGTGGTTCATGCCATGAGAGCTGGATTGGGGCCTCAGGTGACAGCTCAAAAGATTGCAGCCTTGGCACGCCAACGAGCGCAGGACAGAGACCGGCAGACACCTTTCTCTACTGCTGCTCAAGATGCTGGGTTCCGTTACTATGGCGGCAAGCTCGATGATATCACTGTTGTAGTTTCCTATGTTACCAGTTCAAGCGATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C_2 SpoIIE

Protein Analysis

506

Amino Acids

53.82

Weight (kDa)

6.19

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 335 - 450 2.5e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 480
Acc36I ACCTGC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 620
AccI GTMKAC 1 cut(s) 1075
AccII CGCG 2 cut(s) 268, 632
AciI CCGC 6 cut(s) 182, 268, 381, 675, 825, 1458
AclWI GGATC 8 cut(s) 278, 338, 351, 750, 793, 980, 1134, 1505
AcoI YGGCCR 1 cut(s) 1216
AcsI RAATTY 2 cut(s) 121, 638
AcuI CTGAAG 1 cut(s) 1120
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 3 cut(s) 27, 620, 1214
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 5 cut(s) 279, 532, 723, 1165, 1506
AjnI CCWGG 3 cut(s) 57, 1218, 1243
AluBI AGCT 9 cut(s) 25, 392, 718, 956, 1010, 1212, 1334, 1358, 1465
AluI AGCT 9 cut(s) 25, 392, 718, 956, 1010, 1212, 1334, 1358, 1465
Alw21I GWGCWC 1 cut(s) 1130
Alw26I GTCTC 3 cut(s) 136, 1113, 1395
Alw44I GTGCAC 1 cut(s) 1126
AlwI GGATC 8 cut(s) 278, 338, 351, 750, 793, 980, 1134, 1505
AlwNI CAGNNNCTG 1 cut(s) 1235
Ama87I CYCGRG 1 cut(s) 927
AoxI GGCC 6 cut(s) 55, 296, 542, 1216, 1273, 1343
ApaLI GTGCAC 1 cut(s) 1126
ApeKI GCWGC 8 cut(s) 200, 203, 442, 715, 1150, 1153, 1370, 1427
ApoI RAATTY 2 cut(s) 121, 638
AseI ATTAAT 1 cut(s) 1086
AspLEI GCGC 2 cut(s) 632, 1393
AspS9I GGNCC 4 cut(s) 296, 542, 672, 1343
AsuC2I CCSGG 2 cut(s) 186, 416
AsuHPI GGTGA 2 cut(s) 1106, 1363
AvaI CYCGRG 1 cut(s) 927
AvaII GGWCC 1 cut(s) 672
AxyI CCTNAGG 2 cut(s) 708, 1347
BaeGI GKGCMC 2 cut(s) 191, 1130
BalI TGGCCA 1 cut(s) 1218
BamHI GGATCC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 1126
Bbv12I GWGCWC 1 cut(s) 1130
BbvI GCAGC 8 cut(s) 187, 190, 429, 727, 1162, 1165, 1382, 1414
BccI CCATC 7 cut(s) 13, 292, 497, 591, 810, 872, 1265
BcgI CGANNNNNNTGC 4 cut(s) 178, 212, 1107, 1141
BciT130I CCWGG 3 cut(s) 59, 1220, 1245
BciVI GTATCC 1 cut(s) 412
BcnI CCSGG 2 cut(s) 186, 416
BcoDI GTCTC 3 cut(s) 136, 1113, 1395
BfaI CTAG 5 cut(s) 83, 126, 387, 993, 1209
BfmI CTRYAG 3 cut(s) 33, 729, 1308
BfuAI ACCTGC 1 cut(s) 804
BfuI GTATCC 1 cut(s) 412
BglII AGATCT 1 cut(s) 497
Bme1390I CCNGG 5 cut(s) 59, 186, 416, 1220, 1245
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 927
BmgT120I GGNCC 4 cut(s) 296, 542, 672, 1343
BmiI GGNNCC 6 cut(s) 345, 673, 969, 1036, 1344, 1445
BmrFI CCNGG 5 cut(s) 59, 186, 416, 1220, 1245
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 4 cut(s) 748, 823, 1059, 1426
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 1126
BpmI CTGGAG 2 cut(s) 1000, 1202
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 1416
BpuMI CCSGG 2 cut(s) 186, 416
Bsa29I ATCGAT 1 cut(s) 47
BsaI GGTCTC 2 cut(s) 136, 1395
BsaJI CCNNGG 4 cut(s) 369, 414, 1244, 1374
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 620, 1214
Bse118I RCCGGY 1 cut(s) 1404
Bse1I ACTGG 4 cut(s) 348, 826, 1185, 1500
Bse21I CCTNAGG 2 cut(s) 708, 1347
BseBI CCWGG 3 cut(s) 59, 1220, 1245
BseCI ATCGAT 1 cut(s) 47
BseDI CCNNGG 4 cut(s) 369, 414, 1244, 1374
BseGI GGATG 3 cut(s) 802, 838, 1255
BseLI CCNNNNNNNGG 3 cut(s) 27, 620, 1214
BseMII CTCAG 3 cut(s) 699, 927, 1361
BseNI ACTGG 4 cut(s) 348, 826, 1185, 1500
BseRI GAGGAG 1 cut(s) 725
BseSI GKGCMC 2 cut(s) 191, 1130
BseXI GCAGC 8 cut(s) 187, 190, 429, 727, 1162, 1165, 1382, 1414
BseYI CCCAGC 3 cut(s) 668, 891, 1439
BsgI GTGCAG 1 cut(s) 1169
Bsh1236I CGCG 2 cut(s) 268, 632
BshFI GGCC 6 cut(s) 57, 298, 544, 1218, 1275, 1345
BshVI ATCGAT 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 1130
BsiHKCI CYCGRG 1 cut(s) 927
BsiSI CCGG 3 cut(s) 185, 416, 1405
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 3 cut(s) 27, 620, 1214
BsmAI GTCTC 3 cut(s) 136, 1113, 1395
BsmBI CGTCTC 1 cut(s) 1113
BsmFI GGGAC 1 cut(s) 685
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 6 cut(s) 57, 298, 544, 1218, 1275, 1345
Bso31I GGTCTC 2 cut(s) 136, 1395
BsoBI CYCGRG 1 cut(s) 927
Bsp1286I GDGCHC 2 cut(s) 191, 1130
Bsp1407I TGTACA 1 cut(s) 470
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 6 cut(s) 182, 268, 381, 675, 825, 1458
BspANI GGCC 6 cut(s) 57, 298, 544, 1218, 1275, 1345
BspCNI CTCAG 3 cut(s) 700, 926, 1360
BspDI ATCGAT 1 cut(s) 47
BspFNI CGCG 2 cut(s) 268, 632
BspLI GGNNCC 6 cut(s) 345, 673, 969, 1036, 1344, 1445
BspMAI CTGCAG 2 cut(s) 37, 1312
BspMI ACCTGC 1 cut(s) 804
BspPI GGATC 8 cut(s) 278, 338, 351, 750, 793, 980, 1134, 1505
BspTNI GGTCTC 2 cut(s) 136, 1395
BsrFI RCCGGY 1 cut(s) 1404
BsrGI TGTACA 1 cut(s) 470
BsrI ACTGG 4 cut(s) 348, 826, 1185, 1500
BssAI RCCGGY 1 cut(s) 1404
BssECI CCNNGG 4 cut(s) 369, 414, 1244, 1374
BssNAI GTATAC 1 cut(s) 1076
BssT1I CCWWGG 2 cut(s) 369, 1374
Bst1107I GTATAC 1 cut(s) 1076
Bst2UI CCWGG 3 cut(s) 59, 1220, 1245
Bst4CI ACNGT 5 cut(s) 193, 1132, 1199, 1231, 1480
Bst6I CTCTTC 1 cut(s) 587
BstAUI TGTACA 1 cut(s) 470
BstC8I GCNNGC 4 cut(s) 520, 1048, 1381, 1463
BstDEI CTNAG 5 cut(s) 26, 708, 776, 913, 1347
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 3 cut(s) 802, 838, 1255
BstFNI CGCG 2 cut(s) 268, 632
BstHHI GCGC 2 cut(s) 632, 1393
BstMAI GTCTC 3 cut(s) 136, 1113, 1395
BstMWI GCNNNNNNNGC 7 cut(s) 88, 97, 442, 674, 1331, 1376, 1436
BstNI CCWGG 3 cut(s) 59, 1220, 1245
BstNSI RCATGY 2 cut(s) 1050, 1082
BstSCI CCNGG 5 cut(s) 57, 184, 414, 1218, 1243
BstSFI CTRYAG 3 cut(s) 33, 729, 1308
BstSLI GKGCMC 2 cut(s) 191, 1130
BstUI CGCG 2 cut(s) 268, 632
BstV1I GCAGC 8 cut(s) 187, 190, 429, 727, 1162, 1165, 1382, 1414
BstV2I GAAGAC 1 cut(s) 1126
BstX2I RGATCY 3 cut(s) 343, 497, 1139
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 3 cut(s) 343, 497, 1139
BstZ17I GTATAC 1 cut(s) 1076
Bsu15I ATCGAT 1 cut(s) 47
Bsu36I CCTNAGG 2 cut(s) 708, 1347
BsuI GTATCC 1 cut(s) 412
BsuRI GGCC 6 cut(s) 57, 298, 544, 1218, 1275, 1345
BsuTUI ATCGAT 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 3 cut(s) 802, 838, 1255
BtsIMutI CAGTG 4 cut(s) 189, 819, 1204, 1476
BveI ACCTGC 1 cut(s) 804
Cac8I GCNNGC 4 cut(s) 520, 1048, 1381, 1463
CaiI CAGNNNCTG 1 cut(s) 1235
CfoI GCGC 2 cut(s) 632, 1393
Cfr10I RCCGGY 1 cut(s) 1404
Cfr13I GGNCC 4 cut(s) 296, 542, 672, 1343
ClaI ATCGAT 1 cut(s) 47
Csp6I GTAC 1 cut(s) 471
CspCI CAANNNNNGTGG 2 cut(s) 852, 887
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 5 cut(s) 26, 708, 776, 913, 1347
EaeI YGGCCR 1 cut(s) 1216
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
Eco130I CCWWGG 2 cut(s) 369, 1374
Eco31I GGTCTC 2 cut(s) 136, 1395
Eco32I GATATC 1 cut(s) 1474
Eco47I GGWCC 1 cut(s) 672
Eco57I CTGAAG 1 cut(s) 1120
Eco81I CCTNAGG 2 cut(s) 708, 1347
Eco88I CYCGRG 1 cut(s) 927
EcoO109I RGGNCCY 3 cut(s) 296, 542, 1343
EcoRII CCWGG 3 cut(s) 57, 1218, 1243
EcoRV GATATC 1 cut(s) 1474
EcoT14I CCWWGG 2 cut(s) 369, 1374
EcoT22I ATGCAT 2 cut(s) 175, 1052
ErhI CCWWGG 2 cut(s) 369, 1374
Esp3I CGTCTC 1 cut(s) 1113
FaqI GGGAC 1 cut(s) 685
FauI CCCGC 1 cut(s) 818
FauNDI CATATG 1 cut(s) 845
FblI GTMKAC 1 cut(s) 1075
FokI GGATG 3 cut(s) 789, 845, 1262
FspBI CTAG 5 cut(s) 83, 126, 387, 993, 1209
GlaI GCGC 2 cut(s) 631, 1392
GsaI CCCAGC 3 cut(s) 672, 895, 1443
GsuI CTGGAG 2 cut(s) 1000, 1202
HaeIII GGCC 6 cut(s) 57, 298, 544, 1218, 1275, 1345
HapII CCGG 3 cut(s) 185, 416, 1405
HhaI GCGC 2 cut(s) 632, 1393
Hin6I GCGC 2 cut(s) 630, 1391
HinP1I GCGC 2 cut(s) 630, 1391
HincII GTYRAC 3 cut(s) 229, 910, 1195
HindII GTYRAC 3 cut(s) 229, 910, 1195
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 5 cut(s) 77, 215, 329, 734, 1191
HpaI GTTAAC 2 cut(s) 229, 910
HpaII CCGG 3 cut(s) 185, 416, 1405
HphI GGTGA 2 cut(s) 1106, 1363
Hpy166II GTNNAC 6 cut(s) 73, 229, 910, 1076, 1128, 1195
Hpy188I TCNGA 9 cut(s) 42, 163, 214, 343, 360, 502, 742, 747, 1139
Hpy8I GTNNAC 6 cut(s) 73, 229, 910, 1076, 1128, 1195
HpyAV CCTTC 2 cut(s) 56, 309
HpyCH4III ACNGT 5 cut(s) 193, 1132, 1199, 1231, 1480
HpyCH4IV ACGT 1 cut(s) 658
HpyF10VI GCNNNNNNNGC 7 cut(s) 88, 97, 442, 674, 1331, 1376, 1436
HpyF3I CTNAG 5 cut(s) 26, 708, 776, 913, 1347
HpySE526I ACGT 1 cut(s) 658
HspAI GCGC 2 cut(s) 630, 1391
KspAI GTTAAC 2 cut(s) 229, 910
LmnI GCTCC 3 cut(s) 709, 712, 1246
Lsp1109I GCAGC 8 cut(s) 187, 190, 429, 727, 1162, 1165, 1382, 1414
LweI GCATC 4 cut(s) 748, 823, 1059, 1426
MaeI CTAG 5 cut(s) 83, 126, 387, 993, 1209
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 6 cut(s) 453, 605, 949, 1351, 1448, 1495
MboII GAAGA 5 cut(s) 544, 604, 642, 760, 1126
MflI RGATCY 3 cut(s) 343, 497, 1139
MhlI GDGCHC 2 cut(s) 191, 1130
MlsI TGGCCA 1 cut(s) 1218
MluCI AATT 9 cut(s) 93, 107, 121, 638, 646, 697, 943, 1083, 1087
MluNI TGGCCA 1 cut(s) 1218
MlyI GAGTC 2 cut(s) 71, 1200
MmeI TCCRAC 2 cut(s) 192, 981
Mox20I TGGCCA 1 cut(s) 1218
Mph1103I ATGCAT 2 cut(s) 175, 1052
MscI TGGCCA 1 cut(s) 1218
MseI TTAA 5 cut(s) 228, 425, 770, 909, 1086
MslI CAYNNNNRTG 1 cut(s) 848
Msp20I TGGCCA 1 cut(s) 1218
MspA1I CMGCKG 1 cut(s) 956
MspI CCGG 3 cut(s) 185, 416, 1405
MspR9I CCNGG 5 cut(s) 59, 186, 416, 1220, 1245
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 3 cut(s) 59, 1220, 1245
MvnI CGCG 2 cut(s) 268, 632
MwoI GCNNNNNNNGC 7 cut(s) 88, 97, 442, 674, 1331, 1376, 1436
NciI CCSGG 2 cut(s) 186, 416
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 1 cut(s) 845
NlaIV GGNNCC 6 cut(s) 345, 673, 969, 1036, 1344, 1445
NmuCI GTSAC 1 cut(s) 1351
NsiI ATGCAT 2 cut(s) 175, 1052
NspI RCATGY 2 cut(s) 1050, 1082
PaeI GCATGC 1 cut(s) 1050
PcsI WCGNNNNNNNCGW 1 cut(s) 1128
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 215, 329, 734
PflMI CCANNNNNTGG 1 cut(s) 620
PleI GAGTC 2 cut(s) 71, 1199
PpsI GAGTC 2 cut(s) 71, 1199
PshBI ATTAAT 1 cut(s) 1086
PsiI TTATAA 1 cut(s) 480
Psp6I CCWGG 3 cut(s) 57, 1218, 1243
PspFI CCCAGC 3 cut(s) 668, 891, 1439
PspGI CCWGG 3 cut(s) 57, 1218, 1243
PspN4I GGNNCC 6 cut(s) 345, 673, 969, 1036, 1344, 1445
PspPI GGNCC 4 cut(s) 296, 542, 672, 1343
PsrI GAACNNNNNNTAC 4 cut(s) 942, 974, 1059, 1091
PstI CTGCAG 2 cut(s) 37, 1312
PstNI CAGNNNCTG 1 cut(s) 1235
PsuI RGATCY 3 cut(s) 343, 497, 1139
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 848
SaqAI TTAA 5 cut(s) 228, 425, 770, 909, 1086
Sau96I GGNCC 4 cut(s) 296, 542, 672, 1343
SchI GAGTC 2 cut(s) 71, 1200
ScrFI CCNGG 5 cut(s) 59, 186, 416, 1220, 1245
SduI GDGCHC 2 cut(s) 191, 1130
SfaNI GCATC 4 cut(s) 748, 823, 1059, 1426
SfcI CTRYAG 3 cut(s) 33, 729, 1308
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 1 cut(s) 1431
SmoI CTYRAG 1 cut(s) 1431
SphI GCATGC 1 cut(s) 1050
Sse9I AATT 9 cut(s) 93, 107, 121, 638, 646, 697, 943, 1083, 1087
SsiI CCGC 6 cut(s) 182, 268, 381, 675, 825, 1458
SspMI CTAG 5 cut(s) 83, 126, 387, 993, 1209
StyD4I CCNGG 5 cut(s) 57, 184, 414, 1218, 1243
StyI CCWWGG 2 cut(s) 369, 1374
TaaI ACNGT 5 cut(s) 193, 1132, 1199, 1231, 1480
TaiI ACGT 1 cut(s) 661
TaqI TCGA 4 cut(s) 47, 140, 1117, 1467
TasI AATT 9 cut(s) 93, 107, 121, 638, 646, 697, 943, 1083, 1087
TatI WGTACW 1 cut(s) 470
TauI GCSGC 2 cut(s) 184, 1461
TfiI GAWTC 3 cut(s) 215, 329, 734
Tru1I TTAA 5 cut(s) 228, 425, 770, 909, 1086
Tru9I TTAA 5 cut(s) 228, 425, 770, 909, 1086
TscAI CASTG 4 cut(s) 196, 826, 1204, 1483
TseFI GTSAC 1 cut(s) 1351
TseI GCWGC 8 cut(s) 200, 203, 442, 715, 1150, 1153, 1370, 1427
Tsp45I GTSAC 1 cut(s) 1351
TspDTI ATGAA 7 cut(s) 438, 597, 632, 726, 832, 870, 1310
TspGWI ACGGA 1 cut(s) 1436
TspRI CASTG 4 cut(s) 196, 826, 1204, 1483
Van91I CCANNNNNTGG 1 cut(s) 620
VneI GTGCAC 1 cut(s) 1126
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 1 cut(s) 1086
XapI RAATTY 2 cut(s) 121, 638
XbaI TCTAGA 1 cut(s) 386
XceI RCATGY 2 cut(s) 1050, 1082
XmiI GTMKAC 1 cut(s) 1075
XspI CTAG 5 cut(s) 83, 126, 387, 993, 1209
Zsp2I ATGCAT 2 cut(s) 175, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.