MD15G1029400.v1.1

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008377
Physical Location & Seq
Reverse (-)
1792884 .. 1796211
3328 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1029400.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGCCATCTTCATATTTGTCGAAGATTAGGACTGCGTTGCAGAGATCAATTGTTGGGCAGGAAGGAGCGCTTCAGGAATCGGTAGATGGGTTAATTGGGCAGGGCAAATTGTTGTTTGGAAATTCTAAGTTGTTCCAGTCGAGATCGTTTTCAACTATTTCAGACCTCCAAGCATTGTTTTCACCAGGCATTGTTTTCGCTGCAAGATCGGATTCACAGCTAGTTAACCAAAGAAGGAACATTTCTGTTGTAGGAGAGATTTCGCGCATTATTTCAACTCCGTCTGTGTCAGGGCCTTCAATTCAGGTCTGTGGATATCATATCGATTGTGTGCTTTCTGAGCCCAGTCAGTTTGTGGCCAGAAGCAAGTTGCAGAATAAACCTATGGCTGCCTGTGGTTCTAGAACTTTATTTGGGGGATGCTTCCCAGATAATTTAACTTCAAGGCGCGGGATTCATTCAGTGGTACCTGAAAGTGCCTGTACATTATATAACAAAAGAAGTTCCGACTGTTTTCAAACAGCTAGCATGAGTTTGAAAAAAGGTGGGCAATCCAACACTAATGCAATTTATGGGTATTTCATATATGAAGTTGGAAAAAGATTGTGTAATTCCTCCCTAAGTAAAGGATCCGGGTCAAGGGAATTTCATGCTTCATCTACATGCTTATCTACTGGGACTGCCCATGACGTGTCTTTTGATAGTTCTGCTCCTGAGGACCAAGTTTCAAGTTCTGCAGATTCATCTAATGAGGAGGTTACAGATGGAAAGTCCCTAAAACTAACTTCTGGATCATACTACCTACCCCATCCTGACAAAGAGGAAACTGGCGGGGAGGATGCTCACTTCATTTGTGCGAATGAACAAGCAATAGGGGTTGCAGATGGTGTTGGTGGCTGGGCAGATCTTGGTGTTAATTCCGGATTGTACTCCAGAGAACTGATGTCTAATTCTGTAGCTGCTGTTCAGGAGGAGCCGAAGGGGTCCGTTGACCCTGCTCGGGTATTGGAGAAAGCTCACTCGAGCACGAAAGCCAGGGGTTCCTCAACAGCATGTATCATAGCACTCACTGAACAGGGTATTCATGCAGTTAATTTAGGTGATAGTGGGTTTATAGTGGTTCGAGATGGGTGCACCGTCTTTAGATCCCCTGTGCAACAGCATGATTTTAACTTTACCTATCAACTGGAGAGCGGAAACAACGGTGATCTACCTAGCTCTGGCCAGGTTTTTACAGTTCCTGTTGCACCGGGGGATGTTATAATTGCAGGAACGGATGGCCTGTTCGACAACTTGTACAACAACGAGATAACCGCAGTGGTAGTTCATGCCATCAGAGCTGGCTTAGGGCCTCAGGTGACAGCACAGAAGATAGCAGCGTTGGCACGCCAACGAGCACAGGATAGAGACCGGCAGACGCCTTTCTCCACAGCCGCTCAAGATGCTGGGTTCCGTTATTATGGCGGCAAGCTTGATGATATAACTGTTGTTGTTTCATATGTAACCAGCTCAAATGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C_2 SpoIIE

Protein Analysis

508

Amino Acids

53.81

Weight (kDa)

5.84

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 337 - 439 5e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1262
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 1 cut(s) 466
AccBSI CCGCTC 2 cut(s) 1194, 1436
AccII CGCG 2 cut(s) 265, 450
AccIII TCCGGA 1 cut(s) 920
AciI CCGC 6 cut(s) 450, 831, 1194, 1314, 1434, 1464
AclWI GGATC 4 cut(s) 624, 637, 799, 1140
AcoI YGGCCR 2 cut(s) 357, 1222
AcsI RAATTY 2 cut(s) 121, 644
AcuI CTGAAG 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 1418
AfaI GTAC 4 cut(s) 468, 484, 929, 1298
AfeI AGCGCT 1 cut(s) 69
AfiI CCNNNNNNNGG 3 cut(s) 639, 1000, 1220
AflIII ACRYGT 1 cut(s) 690
AgsI TTSAA 7 cut(s) 153, 276, 300, 444, 518, 538, 729
AjiI CACGTC 1 cut(s) 691
AjnI CCWGG 3 cut(s) 184, 1034, 1224
AloI GAACNNNNNNTCC 2 cut(s) 1268, 1300
AluBI AGCT 8 cut(s) 220, 524, 959, 1016, 1218, 1340, 1471, 1509
AluI AGCT 8 cut(s) 220, 524, 959, 1016, 1218, 1340, 1471, 1509
Alw21I GWGCWC 3 cut(s) 1028, 1136, 1399
Alw26I GTCTC 1 cut(s) 1401
Alw44I GTGCAC 1 cut(s) 1132
AlwI GGATC 4 cut(s) 624, 637, 799, 1140
AlwNI CAGNNNCTG 1 cut(s) 1241
Ama87I CYCGRG 2 cut(s) 999, 1021
Aor13HI TCCGGA 1 cut(s) 920
Aor51HI AGCGCT 1 cut(s) 69
AoxI GGCC 5 cut(s) 293, 357, 1222, 1279, 1349
ApaLI GTGCAC 1 cut(s) 1132
ApeKI GCWGC 4 cut(s) 200, 389, 959, 1376
ApoI RAATTY 2 cut(s) 121, 644
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
Asp718I GGTACC 1 cut(s) 466
AspLEI GCGC 3 cut(s) 70, 267, 450
AspS9I GGNCC 4 cut(s) 293, 718, 984, 1349
AsuC2I CCSGG 2 cut(s) 634, 1251
AsuHPI GGTGA 4 cut(s) 174, 1112, 1217, 1369
AsuNHI GCTAGC 1 cut(s) 524
AvaI CYCGRG 2 cut(s) 999, 1021
AvaII GGWCC 2 cut(s) 718, 984
AxyI CCTNAGG 2 cut(s) 714, 1353
BaeGI GKGCMC 1 cut(s) 1136
BalI TGGCCA 2 cut(s) 359, 1224
BamHI GGATCC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 3 cut(s) 1028, 1136, 1399
BbvI GCAGC 4 cut(s) 187, 376, 946, 1388
BccI CCATC 8 cut(s) 13, 80, 758, 816, 878, 1121, 1271, 1340
BcgI CGANNNNNNTGC 4 cut(s) 178, 212, 1113, 1147
BciT130I CCWGG 3 cut(s) 186, 1036, 1226
BcnI CCSGG 2 cut(s) 634, 1251
BcoDI GTCTC 1 cut(s) 1401
BfaI CTAG 4 cut(s) 221, 402, 525, 1215
BfmI CTRYAG 2 cut(s) 735, 954
BfoI RGCGCY 1 cut(s) 71
BglII AGATCT 1 cut(s) 904
BisI GCNGC 6 cut(s) 201, 390, 960, 1377, 1434, 1465
BlsI GCNGC 6 cut(s) 202, 391, 961, 1378, 1435, 1466
Bme1390I CCNGG 5 cut(s) 186, 634, 1036, 1226, 1251
Bme18I GGWCC 2 cut(s) 718, 984
BmeT110I CYCGRG 2 cut(s) 999, 1021
BmgBI CACGTC 1 cut(s) 691
BmgT120I GGNCC 4 cut(s) 293, 718, 984, 1349
BmiI GGNNCC 6 cut(s) 468, 631, 975, 985, 1042, 1451
BmrFI CCNGG 5 cut(s) 186, 634, 1036, 1226, 1251
BmrI ACTGGG 2 cut(s) 339, 684
BmsI GCATC 4 cut(s) 410, 829, 1432, 1507
BmtI GCTAGC 1 cut(s) 528
BmuI ACTGGG 2 cut(s) 339, 684
BpmI CTGGAG 2 cut(s) 916, 1208
Bpu10I CCTNAGC 1 cut(s) 1345
BpuEI CTTGAG 1 cut(s) 1422
BpuMI CCSGG 2 cut(s) 634, 1251
Bsa29I ATCGAT 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 1418
BsaI GGTCTC 1 cut(s) 1401
BsaJI CCNNGG 2 cut(s) 1035, 1250
BsaWI WCCGGW 1 cut(s) 920
Bsc4I CCNNNNNNNGG 3 cut(s) 639, 1000, 1220
Bse118I RCCGGY 1 cut(s) 1410
Bse1I ACTGG 5 cut(s) 136, 345, 679, 832, 1191
Bse21I CCTNAGG 2 cut(s) 714, 1353
BseAI TCCGGA 1 cut(s) 920
BseBI CCWGG 3 cut(s) 186, 1036, 1226
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 2 cut(s) 1035, 1250
BseGI GGATG 5 cut(s) 425, 808, 844, 1261, 1282
BseLI CCNNNNNNNGG 3 cut(s) 639, 1000, 1220
BseMII CTCAG 3 cut(s) 330, 705, 1367
BseNI ACTGG 5 cut(s) 136, 345, 679, 832, 1191
BseRI GAGGAG 2 cut(s) 767, 986
BseSI GKGCMC 1 cut(s) 1136
BseXI GCAGC 4 cut(s) 187, 376, 946, 1388
BseYI CCCAGC 2 cut(s) 897, 1445
Bsh1236I CGCG 2 cut(s) 265, 450
BshFI GGCC 5 cut(s) 295, 359, 1224, 1281, 1351
BshNI GGYRCC 1 cut(s) 466
BshVI ATCGAT 1 cut(s) 324
BsiHKAI GWGCWC 3 cut(s) 1028, 1136, 1399
BsiHKCI CYCGRG 2 cut(s) 999, 1021
BsiSI CCGG 4 cut(s) 633, 921, 1250, 1411
BslFI GGGAC 2 cut(s) 691, 757
BslI CCNNNNNNNGG 3 cut(s) 639, 1000, 1220
BsmAI GTCTC 1 cut(s) 1401
BsmFI GGGAC 2 cut(s) 691, 757
BsnI GGCC 5 cut(s) 295, 359, 1224, 1281, 1351
Bso31I GGTCTC 1 cut(s) 1401
BsoBI CYCGRG 2 cut(s) 999, 1021
Bsp1286I GDGCHC 4 cut(s) 345, 1028, 1136, 1399
Bsp13I TCCGGA 1 cut(s) 920
Bsp1407I TGTACA 2 cut(s) 482, 1296
Bsp143I GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
BspACI CCGC 6 cut(s) 450, 831, 1194, 1314, 1434, 1464
BspANI GGCC 5 cut(s) 295, 359, 1224, 1281, 1351
BspCNI CTCAG 3 cut(s) 331, 706, 1366
BspDI ATCGAT 1 cut(s) 324
BspEI TCCGGA 1 cut(s) 920
BspFNI CGCG 2 cut(s) 265, 450
BspLI GGNNCC 6 cut(s) 468, 631, 975, 985, 1042, 1451
BspMAI CTGCAG 1 cut(s) 739
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 4 cut(s) 624, 637, 799, 1140
BspT107I GGYRCC 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 1401
BsrBI CCGCTC 2 cut(s) 1194, 1436
BsrFI RCCGGY 1 cut(s) 1410
BsrGI TGTACA 2 cut(s) 482, 1296
BsrI ACTGG 5 cut(s) 136, 345, 679, 832, 1191
BssAI RCCGGY 1 cut(s) 1410
BssECI CCNNGG 2 cut(s) 1035, 1250
BssMI GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
BssNI GRCGYC 1 cut(s) 1418
Bst2UI CCWGG 3 cut(s) 186, 1036, 1226
Bst4CI ACNGT 5 cut(s) 512, 1138, 1205, 1237, 1486
BstACI GRCGYC 1 cut(s) 1418
BstAUI TGTACA 2 cut(s) 482, 1296
BstC8I GCNNGC 4 cut(s) 526, 1342, 1387, 1469
BstDEI CTNAG 6 cut(s) 126, 339, 620, 714, 1345, 1353
BstF5I GGATG 5 cut(s) 425, 808, 844, 1261, 1282
BstFNI CGCG 2 cut(s) 265, 450
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 3 cut(s) 70, 267, 450
BstKTI GATC 8 cut(s) 47, 146, 209, 632, 794, 907, 1148, 1210
BstMAI GTCTC 1 cut(s) 1401
BstMBI GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
BstMWI GCNNNNNNNGC 4 cut(s) 340, 1337, 1382, 1442
BstNI CCWGG 3 cut(s) 186, 1036, 1226
BstNSI RCATGY 2 cut(s) 666, 1056
BstSCI CCNGG 5 cut(s) 184, 632, 1034, 1224, 1249
BstSFI CTRYAG 2 cut(s) 735, 954
BstSLI GKGCMC 1 cut(s) 1136
BstUI CGCG 2 cut(s) 265, 450
BstV1I GCAGC 4 cut(s) 187, 376, 946, 1388
BstX2I RGATCY 3 cut(s) 629, 904, 1145
BstYI RGATCY 3 cut(s) 629, 904, 1145
Bsu15I ATCGAT 1 cut(s) 324
Bsu36I CCTNAGG 2 cut(s) 714, 1353
BsuRI GGCC 5 cut(s) 295, 359, 1224, 1281, 1351
BsuTUI ATCGAT 1 cut(s) 324
BtrI CACGTC 1 cut(s) 691
BtsCI GGATG 5 cut(s) 425, 808, 844, 1261, 1282
BtsI GCAGTG 1 cut(s) 1323
BtsIMutI CAGTG 3 cut(s) 468, 1068, 1323
Cac8I GCNNGC 4 cut(s) 526, 1342, 1387, 1469
CaiI CAGNNNCTG 1 cut(s) 1241
CfoI GCGC 3 cut(s) 70, 267, 450
Cfr10I RCCGGY 1 cut(s) 1410
Cfr13I GGNCC 4 cut(s) 293, 718, 984, 1349
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 1 cut(s) 1426
Csp6I GTAC 4 cut(s) 467, 483, 928, 1297
CviAII CATG 9 cut(s) 529, 650, 663, 686, 1053, 1085, 1163, 1328, 1521
CviQI GTAC 4 cut(s) 467, 483, 928, 1297
DdeI CTNAG 6 cut(s) 126, 339, 620, 714, 1345, 1353
DpnI GATC 8 cut(s) 46, 145, 208, 631, 793, 906, 1147, 1209
DpnII GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
EaeI YGGCCR 2 cut(s) 357, 1222
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 1401
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 718, 984
Eco47III AGCGCT 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 56
Eco81I CCTNAGG 2 cut(s) 714, 1353
Eco88I CYCGRG 2 cut(s) 999, 1021
EcoO109I RGGNCCY 2 cut(s) 293, 1349
EcoRII CCWGG 3 cut(s) 184, 1034, 1224
EcoRV GATATC 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 1522
EcoT38I GRGCYC 1 cut(s) 345
FaeI CATG 9 cut(s) 532, 653, 666, 689, 1056, 1088, 1166, 1331, 1524
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FaqI GGGAC 2 cut(s) 691, 757
FatI CATG 9 cut(s) 528, 649, 662, 685, 1052, 1084, 1162, 1327, 1520
FauI CCCGC 2 cut(s) 443, 824
FauNDI CATATG 1 cut(s) 1498
Fnu4HI GCNGC 6 cut(s) 201, 390, 960, 1377, 1434, 1465
FokI GGATG 5 cut(s) 432, 795, 851, 1268, 1289
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 6 cut(s) 201, 390, 960, 1377, 1434, 1465
FspBI CTAG 4 cut(s) 221, 402, 525, 1215
GlaI GCGC 3 cut(s) 69, 266, 449
GluI GCNGC 6 cut(s) 201, 390, 960, 1377, 1434, 1465
GsaI CCCAGC 2 cut(s) 901, 1449
GsuI CTGGAG 2 cut(s) 916, 1208
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 5 cut(s) 295, 359, 1224, 1281, 1351
HapII CCGG 4 cut(s) 633, 921, 1250, 1411
HgaI GACGC 1 cut(s) 1426
HhaI GCGC 3 cut(s) 70, 267, 450
Hin1I GRCGYC 1 cut(s) 1418
Hin1II CATG 9 cut(s) 532, 653, 666, 689, 1056, 1088, 1166, 1331, 1524
Hin6I GCGC 3 cut(s) 68, 265, 448
HinP1I GCGC 3 cut(s) 68, 265, 448
HincII GTYRAC 2 cut(s) 226, 991
HindII GTYRAC 2 cut(s) 226, 991
HindIII AAGCTT 1 cut(s) 1469
HinfI GANTC 4 cut(s) 77, 212, 454, 740
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 4 cut(s) 633, 921, 1250, 1411
HphI GGTGA 4 cut(s) 174, 1112, 1217, 1369
Hpy166II GTNNAC 3 cut(s) 226, 991, 1134
Hpy188I TCNGA 5 cut(s) 163, 211, 340, 508, 1337
Hpy8I GTNNAC 3 cut(s) 226, 991, 1134
HpyAV CCTTC 4 cut(s) 56, 228, 306, 973
HpyCH4III ACNGT 5 cut(s) 512, 1138, 1205, 1237, 1486
HpyCH4IV ACGT 1 cut(s) 690
HpyF10VI GCNNNNNNNGC 4 cut(s) 340, 1337, 1382, 1442
HpyF3I CTNAG 6 cut(s) 126, 339, 620, 714, 1345, 1353
HpySE526I ACGT 1 cut(s) 690
Hsp92I GRCGYC 1 cut(s) 1418
Hsp92II CATG 9 cut(s) 532, 653, 666, 689, 1056, 1088, 1166, 1331, 1524
HspAI GCGC 3 cut(s) 68, 265, 448
Kpn2I TCCGGA 1 cut(s) 920
KpnI GGTACC 1 cut(s) 470
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
LmnI GCTCC 3 cut(s) 65, 715, 973
Lsp1109I GCAGC 4 cut(s) 187, 376, 946, 1388
LweI GCATC 4 cut(s) 410, 829, 1432, 1507
MaeI CTAG 4 cut(s) 221, 402, 525, 1215
MaeII ACGT 1 cut(s) 690
MaeIII GTNAC 3 cut(s) 757, 1357, 1501
MalI GATC 8 cut(s) 46, 145, 208, 631, 793, 906, 1147, 1209
MbiI CCGCTC 2 cut(s) 1194, 1436
MboI GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
MboII GAAGA 2 cut(s) 34, 1381
MfeI CAATTG 1 cut(s) 48
MflI RGATCY 3 cut(s) 629, 904, 1145
MhlI GDGCHC 4 cut(s) 345, 1028, 1136, 1399
MlsI TGGCCA 2 cut(s) 359, 1224
MluNI TGGCCA 2 cut(s) 359, 1224
MmeI TCCRAC 3 cut(s) 531, 574, 579
Mox20I TGGCCA 2 cut(s) 359, 1224
Mph1103I ATGCAT 1 cut(s) 1522
MroI TCCGGA 1 cut(s) 920
MscI TGGCCA 2 cut(s) 359, 1224
MseI TTAA 6 cut(s) 92, 225, 437, 915, 1092, 1170
MslI CAYNNNNRTG 1 cut(s) 661
Msp20I TGGCCA 2 cut(s) 359, 1224
MspI CCGG 4 cut(s) 633, 921, 1250, 1411
MspR9I CCNGG 5 cut(s) 186, 634, 1036, 1226, 1251
MunI CAATTG 1 cut(s) 48
MvaI CCWGG 3 cut(s) 186, 1036, 1226
MvnI CGCG 2 cut(s) 265, 450
MwoI GCNNNNNNNGC 4 cut(s) 340, 1337, 1382, 1442
NciI CCSGG 2 cut(s) 634, 1251
NdeI CATATG 1 cut(s) 1498
NdeII GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 9 cut(s) 532, 653, 666, 689, 1056, 1088, 1166, 1331, 1524
NlaIV GGNNCC 6 cut(s) 468, 631, 975, 985, 1042, 1451
NmuCI GTSAC 1 cut(s) 1357
NsiI ATGCAT 1 cut(s) 1522
NspI RCATGY 2 cut(s) 666, 1056
PaeR7I CTCGAG 1 cut(s) 1021
PfeI GAWTC 4 cut(s) 77, 212, 454, 740
PkrI GCNGC 6 cut(s) 202, 391, 961, 1378, 1435, 1466
PsiI TTATAA 1 cut(s) 1262
Psp6I CCWGG 3 cut(s) 184, 1034, 1224
PspFI CCCAGC 2 cut(s) 897, 1445
PspGI CCWGG 3 cut(s) 184, 1034, 1224
PspN4I GGNNCC 6 cut(s) 468, 631, 975, 985, 1042, 1451
PspPI GGNCC 4 cut(s) 293, 718, 984, 1349
PspXI VCTCGAGB 1 cut(s) 1021
PsrI GAACNNNNNNTAC 2 cut(s) 948, 980
PstI CTGCAG 1 cut(s) 739
PstNI CAGNNNCTG 1 cut(s) 1241
PsuI RGATCY 3 cut(s) 629, 904, 1145
RsaI GTAC 4 cut(s) 468, 484, 929, 1298
RsaNI GTAC 4 cut(s) 467, 483, 928, 1297
RseI CAYNNNNRTG 1 cut(s) 661
SaqAI TTAA 6 cut(s) 92, 225, 437, 915, 1092, 1170
SatI GCNGC 6 cut(s) 201, 390, 960, 1377, 1434, 1465
Sau3AI GATC 8 cut(s) 44, 143, 206, 629, 791, 904, 1145, 1207
Sau96I GGNCC 4 cut(s) 293, 718, 984, 1349
ScrFI CCNGG 5 cut(s) 186, 634, 1036, 1226, 1251
SduI GDGCHC 4 cut(s) 345, 1028, 1136, 1399
SfaNI GCATC 4 cut(s) 410, 829, 1432, 1507
SfcI CTRYAG 2 cut(s) 735, 954
Sfr274I CTCGAG 1 cut(s) 1021
SinI GGWCC 2 cut(s) 718, 984
SlaI CTCGAG 1 cut(s) 1021
SmiMI CAYNNNNRTG 1 cut(s) 661
SmlI CTYRAG 2 cut(s) 1021, 1437
SmoI CTYRAG 2 cut(s) 1021, 1437
SsiI CCGC 6 cut(s) 450, 831, 1194, 1314, 1434, 1464
SspMI CTAG 4 cut(s) 221, 402, 525, 1215
StyD4I CCNGG 5 cut(s) 184, 632, 1034, 1224, 1249
TaaI ACNGT 5 cut(s) 512, 1138, 1205, 1237, 1486
TaiI ACGT 1 cut(s) 693
TaqI TCGA 6 cut(s) 20, 140, 324, 1022, 1123, 1287
TatI WGTACW 3 cut(s) 482, 927, 1296
TauI GCSGC 2 cut(s) 1436, 1467
TfiI GAWTC 4 cut(s) 77, 212, 454, 740
Tru1I TTAA 6 cut(s) 92, 225, 437, 915, 1092, 1170
Tru9I TTAA 6 cut(s) 92, 225, 437, 915, 1092, 1170
TscAI CASTG 3 cut(s) 468, 1075, 1323
TseFI GTSAC 1 cut(s) 1357
TseI GCWGC 4 cut(s) 200, 389, 959, 1376
Tsp45I GTSAC 1 cut(s) 1357
TspGWI ACGGA 4 cut(s) 270, 976, 1289, 1442
TspRI CASTG 3 cut(s) 468, 1075, 1323
VneI GTGCAC 1 cut(s) 1132
VpaK11BI GGWCC 2 cut(s) 718, 984
XapI RAATTY 2 cut(s) 121, 644
XbaI TCTAGA 1 cut(s) 401
XceI RCATGY 2 cut(s) 666, 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 352
XhoI CTCGAG 1 cut(s) 1021
XspI CTAG 4 cut(s) 221, 402, 525, 1215
Zsp2I ATGCAT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.