Rroxscaffold_7G00167520

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008377
Physical Location & Seq
Forward (+)
8633034 .. 8635510
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167520.1

Sequence Viewer

Length: 1413 bp
ATGCCATCTTCTTATTTATCGAAGCTTAGGACTGCAGTACAGAGGTCGATTGTAGGCCAGGAAGGTGTTGTTCACGACTCGCTAGAGGGGCTAATTGGGCAAGGCAAATTGTTGTTTGGAAATTCTAGGCTGTTCCATTCGAGACCCTTTTCGGCTATTTCAGACCTGAATGCATTTCTGCCGCCGGGCACTGTTGTCGCTGCAAGGTCTGATTCGCAGTTAGTTAACCAGAGGAAGCAGATTTCTGTTGTTGGGGAGATTTCGCGGATCATTTCAACTCCATCTGTGTCGGGGCCTTCGTTCCAAGTTTTTGGGTATCATATTGATTCTGTGCTTTCGGATCCCAGTCAGTTTCAGACTAAAGCCATGGCTTTGTGCGGCAGAAGTAAAAGTTCTGATGGTTGCCAGAAAGCAAGCATGAGTTTGAAGAACAAAGGGCCTCCCAACACTAATGCAACTTACGGATATTTCGTATATGAAGTTGGGAAGAGATGGTGTAGTAACTTCCCAATAAATGGATTAGGCTCGAGAGAATTTCATAATTCTTCCACATGCTTCTCTGCTGGGACTGCCCACGATGTGTCTTTTGATAATTCTGCTCCTGAGGAGCAGCTTTCAAGTTCTACAGATTCATCGGATCAGAAGAACACAGATGCAAAGTCCTTAAAACTAAGTTCAGGATCATGCTACCTGCCCCATCCTGATAAAGAGGACACTGGCGGGGAGGATGCTCACTTCATATGTGTGAATGAACAAGCAATAGGGGTGGCAGATGGTGTTGGTGGCTGGGCAGAGCATGGTGTTAACTCAGGATTGTATTCCCGAGAACTGATGTCTAATTCTGTAACAGCCGTTCAGGAGGAACCCAAAGGTTCAGTTGATCCTGCTAGGGTGTTGGAGAAAGCTCACTCCAGCACAAAAGCCAGAGGTTCCTCAACAGCATGCATCATAGCACTCACAGAACAGGGTATACATGCAATTAATTTAGGTGATAGTGGGTTTATAGTTGTTCGAGACGGGTGCACCGTCTTCAGATCCCCTGTGCAGCAGCATGATTTCAACTTCACTTATCAACTGGAGAATGGAGTCAACAGTGATCTACCTAGCTCTGGCCAGGTTTTTACAGTTCCTGTTGCTCCTGGGGATGTTATAGTTGCTGGAACTGATGGCCTGTTTGACAACTTGTATAACAATGAGATTACTGCAGTTGTGGTTCATGCCATGAGAGCTGGATTAGGGCCTCAGGTGACAGCTCAAAAGATTGCAGCCTTGGCACGCCAACGAGCGCAGGACAGAGACCGGCAGACACCTTTCTCTACTGCTGCTCAAGATGCTGGGTTCCGTTACTATGGCGGCAAGCTCGATGATATCACTGTTGTAGTTTCCTATGTTACCAGTTCAAGCGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C_2 SpoIIE

Protein Analysis

470

Amino Acids

50.08

Weight (kDa)

5.85

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 300 - 415 1.8e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 699
AccB7I CCANNNNNTGG 1 cut(s) 515
AccI GTMKAC 1 cut(s) 970
AccII CGCG 1 cut(s) 265
AciI CCGC 5 cut(s) 182, 265, 378, 720, 1353
AclWI GGATC 7 cut(s) 275, 335, 348, 645, 688, 875, 1029
AcoI YGGCCR 1 cut(s) 1111
AcsI RAATTY 2 cut(s) 121, 533
AcuI CTGAAG 1 cut(s) 1015
AdeI CACNNNGTG 1 cut(s) 580
AfaI GTAC 1 cut(s) 39
AfiI CCNNNNNNNGG 2 cut(s) 515, 1109
AgsI TTSAA 5 cut(s) 276, 427, 618, 1060, 1401
AjnI CCWGG 3 cut(s) 57, 1113, 1138
AluBI AGCT 7 cut(s) 25, 613, 905, 1107, 1229, 1253, 1360
AluI AGCT 7 cut(s) 25, 613, 905, 1107, 1229, 1253, 1360
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 3 cut(s) 136, 1008, 1290
Alw44I GTGCAC 1 cut(s) 1021
AlwI GGATC 7 cut(s) 275, 335, 348, 645, 688, 875, 1029
AlwNI CAGNNNCTG 1 cut(s) 1130
Ama87I CYCGRG 2 cut(s) 526, 822
AoxI GGCC 6 cut(s) 55, 293, 437, 1111, 1168, 1238
ApaLI GTGCAC 1 cut(s) 1021
ApeKI GCWGC 6 cut(s) 200, 610, 1045, 1048, 1265, 1322
ApoI RAATTY 2 cut(s) 121, 533
AseI ATTAAT 1 cut(s) 981
AspLEI GCGC 1 cut(s) 1288
AspS9I GGNCC 3 cut(s) 293, 437, 1238
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 2 cut(s) 1001, 1258
AvaI CYCGRG 2 cut(s) 526, 822
AxyI CCTNAGG 2 cut(s) 603, 1242
BaeGI GKGCMC 2 cut(s) 191, 1025
BalI TGGCCA 1 cut(s) 1113
BamHI GGATCC 1 cut(s) 340
BbsI GAAGAC 1 cut(s) 1021
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 6 cut(s) 187, 622, 1057, 1060, 1277, 1309
BccI CCATC 7 cut(s) 13, 289, 392, 486, 705, 767, 1160
BceAI ACGGC 1 cut(s) 836
BcgI CGANNNNNNTGC 4 cut(s) 178, 212, 1002, 1036
BciT130I CCWGG 3 cut(s) 59, 1115, 1140
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 3 cut(s) 136, 1008, 1290
BfaI CTAG 4 cut(s) 83, 126, 888, 1104
BfmI CTRYAG 3 cut(s) 33, 624, 1203
BfuAI ACCTGC 1 cut(s) 699
BisI GCNGC 9 cut(s) 182, 201, 379, 611, 1046, 1049, 1266, 1323, 1354
BlsI GCNGC 9 cut(s) 183, 202, 380, 612, 1047, 1050, 1267, 1324, 1355
Bme1390I CCNGG 4 cut(s) 59, 186, 1115, 1140
BmeT110I CYCGRG 2 cut(s) 526, 822
BmgT120I GGNCC 3 cut(s) 293, 437, 1238
BmiI GGNNCC 5 cut(s) 294, 342, 864, 931, 1340
BmrFI CCNGG 4 cut(s) 59, 186, 1115, 1140
BmrI ACTGGG 1 cut(s) 339
BmsI GCATC 5 cut(s) 643, 718, 954, 1321, 1396
BmuI ACTGGG 1 cut(s) 339
BpiI GAAGAC 1 cut(s) 1021
BpmI CTGGAG 2 cut(s) 895, 1097
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 1311
BpuMI CCSGG 1 cut(s) 186
BsaI GGTCTC 2 cut(s) 136, 1290
BsaJI CCNNGG 3 cut(s) 366, 1139, 1269
Bsc4I CCNNNNNNNGG 2 cut(s) 515, 1109
Bse118I RCCGGY 1 cut(s) 1299
Bse1I ACTGG 4 cut(s) 345, 721, 1080, 1395
Bse21I CCTNAGG 2 cut(s) 603, 1242
BseBI CCWGG 3 cut(s) 59, 1115, 1140
BseDI CCNNGG 3 cut(s) 366, 1139, 1269
BseGI GGATG 3 cut(s) 697, 733, 1150
BseLI CCNNNNNNNGG 2 cut(s) 515, 1109
BseMII CTCAG 3 cut(s) 594, 822, 1256
BseNI ACTGG 4 cut(s) 345, 721, 1080, 1395
BseRI GAGGAG 1 cut(s) 620
BseSI GKGCMC 2 cut(s) 191, 1025
BseXI GCAGC 6 cut(s) 187, 622, 1057, 1060, 1277, 1309
BseYI CCCAGC 3 cut(s) 563, 786, 1334
BsgI GTGCAG 1 cut(s) 1064
Bsh1236I CGCG 1 cut(s) 265
BshFI GGCC 6 cut(s) 57, 295, 439, 1113, 1170, 1240
BsiHKAI GWGCWC 1 cut(s) 1025
BsiHKCI CYCGRG 2 cut(s) 526, 822
BsiSI CCGG 2 cut(s) 185, 1300
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 2 cut(s) 515, 1109
BsmAI GTCTC 3 cut(s) 136, 1008, 1290
BsmBI CGTCTC 1 cut(s) 1008
BsmFI GGGAC 1 cut(s) 580
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 6 cut(s) 57, 295, 439, 1113, 1170, 1240
Bso31I GGTCTC 2 cut(s) 136, 1290
BsoBI CYCGRG 2 cut(s) 526, 822
Bsp1286I GDGCHC 2 cut(s) 191, 1025
Bsp143I GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 5 cut(s) 182, 265, 378, 720, 1353
BspANI GGCC 6 cut(s) 57, 295, 439, 1113, 1170, 1240
BspCNI CTCAG 3 cut(s) 595, 821, 1255
BspFNI CGCG 1 cut(s) 265
BspLI GGNNCC 5 cut(s) 294, 342, 864, 931, 1340
BspMAI CTGCAG 2 cut(s) 37, 1207
BspMI ACCTGC 1 cut(s) 699
BspPI GGATC 7 cut(s) 275, 335, 348, 645, 688, 875, 1029
BspTNI GGTCTC 2 cut(s) 136, 1290
BsrFI RCCGGY 1 cut(s) 1299
BsrI ACTGG 4 cut(s) 345, 721, 1080, 1395
BssAI RCCGGY 1 cut(s) 1299
BssECI CCNNGG 3 cut(s) 366, 1139, 1269
BssMI GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
BssNAI GTATAC 1 cut(s) 971
BssT1I CCWWGG 2 cut(s) 366, 1269
Bst1107I GTATAC 1 cut(s) 971
Bst2UI CCWGG 3 cut(s) 59, 1115, 1140
Bst4CI ACNGT 5 cut(s) 193, 1027, 1094, 1126, 1375
Bst6I CTCTTC 1 cut(s) 482
BstC8I GCNNGC 4 cut(s) 415, 943, 1276, 1358
BstDEI CTNAG 5 cut(s) 26, 603, 671, 808, 1242
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 697, 733, 1150
BstFNI CGCG 1 cut(s) 265
BstHHI GCGC 1 cut(s) 1288
BstKTI GATC 7 cut(s) 270, 343, 640, 683, 883, 1037, 1099
BstMAI GTCTC 3 cut(s) 136, 1008, 1290
BstMBI GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
BstMWI GCNNNNNNNGC 6 cut(s) 88, 97, 569, 1226, 1271, 1331
BstNI CCWGG 3 cut(s) 59, 1115, 1140
BstNSI RCATGY 3 cut(s) 555, 945, 977
BstSCI CCNGG 4 cut(s) 57, 184, 1113, 1138
BstSFI CTRYAG 3 cut(s) 33, 624, 1203
BstSLI GKGCMC 2 cut(s) 191, 1025
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 6 cut(s) 187, 622, 1057, 1060, 1277, 1309
BstV2I GAAGAC 1 cut(s) 1021
BstX2I RGATCY 2 cut(s) 340, 1034
BstXI CCANNNNNNTGG 1 cut(s) 311
BstYI RGATCY 2 cut(s) 340, 1034
BstZ17I GTATAC 1 cut(s) 971
Bsu36I CCTNAGG 2 cut(s) 603, 1242
BsuRI GGCC 6 cut(s) 57, 295, 439, 1113, 1170, 1240
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 3 cut(s) 697, 733, 1150
BtsIMutI CAGTG 4 cut(s) 189, 714, 1099, 1371
BveI ACCTGC 1 cut(s) 699
Cac8I GCNNGC 4 cut(s) 415, 943, 1276, 1358
CaiI CAGNNNCTG 1 cut(s) 1130
CfoI GCGC 1 cut(s) 1288
Cfr10I RCCGGY 1 cut(s) 1299
Cfr13I GGNCC 3 cut(s) 293, 437, 1238
Csp6I GTAC 1 cut(s) 38
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviQI GTAC 1 cut(s) 38
DdeI CTNAG 5 cut(s) 26, 603, 671, 808, 1242
DpnI GATC 7 cut(s) 269, 342, 639, 682, 882, 1036, 1098
DpnII GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
DraIII CACNNNGTG 1 cut(s) 580
EaeI YGGCCR 1 cut(s) 1111
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Eco130I CCWWGG 2 cut(s) 366, 1269
Eco31I GGTCTC 2 cut(s) 136, 1290
Eco32I GATATC 1 cut(s) 1369
Eco57I CTGAAG 1 cut(s) 1015
Eco81I CCTNAGG 2 cut(s) 603, 1242
Eco88I CYCGRG 2 cut(s) 526, 822
EcoO109I RGGNCCY 3 cut(s) 293, 437, 1238
EcoRII CCWGG 3 cut(s) 57, 1113, 1138
EcoRV GATATC 1 cut(s) 1369
EcoT14I CCWWGG 2 cut(s) 366, 1269
EcoT22I ATGCAT 3 cut(s) 175, 947, 1411
ErhI CCWWGG 2 cut(s) 366, 1269
Esp3I CGTCTC 1 cut(s) 1008
FaqI GGGAC 1 cut(s) 580
FauI CCCGC 1 cut(s) 713
FauNDI CATATG 1 cut(s) 740
FblI GTMKAC 1 cut(s) 970
Fnu4HI GCNGC 9 cut(s) 182, 201, 379, 611, 1046, 1049, 1266, 1323, 1354
FokI GGATG 3 cut(s) 684, 740, 1157
Fsp4HI GCNGC 9 cut(s) 182, 201, 379, 611, 1046, 1049, 1266, 1323, 1354
FspBI CTAG 4 cut(s) 83, 126, 888, 1104
GlaI GCGC 1 cut(s) 1287
GluI GCNGC 9 cut(s) 182, 201, 379, 611, 1046, 1049, 1266, 1323, 1354
GsaI CCCAGC 3 cut(s) 567, 790, 1338
GsuI CTGGAG 2 cut(s) 895, 1097
HaeIII GGCC 6 cut(s) 57, 295, 439, 1113, 1170, 1240
HapII CCGG 2 cut(s) 185, 1300
HhaI GCGC 1 cut(s) 1288
Hin6I GCGC 1 cut(s) 1286
HinP1I GCGC 1 cut(s) 1286
HincII GTYRAC 3 cut(s) 226, 805, 1090
HindII GTYRAC 3 cut(s) 226, 805, 1090
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 5 cut(s) 77, 212, 326, 629, 1086
HpaI GTTAAC 2 cut(s) 226, 805
HpaII CCGG 2 cut(s) 185, 1300
HphI GGTGA 2 cut(s) 1001, 1258
Hpy166II GTNNAC 6 cut(s) 73, 226, 805, 971, 1023, 1090
Hpy188I TCNGA 8 cut(s) 163, 211, 340, 357, 397, 637, 642, 1034
Hpy8I GTNNAC 6 cut(s) 73, 226, 805, 971, 1023, 1090
HpyAV CCTTC 2 cut(s) 56, 306
HpyCH4III ACNGT 5 cut(s) 193, 1027, 1094, 1126, 1375
HpyF10VI GCNNNNNNNGC 6 cut(s) 88, 97, 569, 1226, 1271, 1331
HpyF3I CTNAG 5 cut(s) 26, 603, 671, 808, 1242
HspAI GCGC 1 cut(s) 1286
KspAI GTTAAC 2 cut(s) 226, 805
Kzo9I GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
LmnI GCTCC 3 cut(s) 604, 607, 1141
Lsp1109I GCAGC 6 cut(s) 187, 622, 1057, 1060, 1277, 1309
LweI GCATC 5 cut(s) 643, 718, 954, 1321, 1396
MaeI CTAG 4 cut(s) 83, 126, 888, 1104
MaeIII GTNAC 5 cut(s) 500, 844, 1246, 1343, 1390
MalI GATC 7 cut(s) 269, 342, 639, 682, 882, 1036, 1098
MboI GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
MboII GAAGA 5 cut(s) 439, 499, 537, 655, 1021
MflI RGATCY 2 cut(s) 340, 1034
MhlI GDGCHC 2 cut(s) 191, 1025
MlsI TGGCCA 1 cut(s) 1113
MluCI AATT 9 cut(s) 93, 107, 121, 533, 541, 592, 838, 978, 982
MluNI TGGCCA 1 cut(s) 1113
MlyI GAGTC 2 cut(s) 71, 1095
MmeI TCCRAC 1 cut(s) 876
Mox20I TGGCCA 1 cut(s) 1113
Mph1103I ATGCAT 3 cut(s) 175, 947, 1411
MscI TGGCCA 1 cut(s) 1113
MseI TTAA 4 cut(s) 225, 665, 804, 981
MslI CAYNNNNRTG 1 cut(s) 743
Msp20I TGGCCA 1 cut(s) 1113
MspI CCGG 2 cut(s) 185, 1300
MspR9I CCNGG 4 cut(s) 59, 186, 1115, 1140
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 3 cut(s) 59, 1115, 1140
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 6 cut(s) 88, 97, 569, 1226, 1271, 1331
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 740
NdeII GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
NlaIV GGNNCC 5 cut(s) 294, 342, 864, 931, 1340
NmuCI GTSAC 1 cut(s) 1246
NsiI ATGCAT 3 cut(s) 175, 947, 1411
NspI RCATGY 3 cut(s) 555, 945, 977
PaeI GCATGC 1 cut(s) 945
PaeR7I CTCGAG 1 cut(s) 526
PcsI WCGNNNNNNNCGW 3 cut(s) 296, 468, 1023
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 212, 326, 629
PflMI CCANNNNNTGG 1 cut(s) 515
PkrI GCNGC 9 cut(s) 183, 202, 380, 612, 1047, 1050, 1267, 1324, 1355
PleI GAGTC 2 cut(s) 71, 1094
PpsI GAGTC 2 cut(s) 71, 1094
PshBI ATTAAT 1 cut(s) 981
Psp6I CCWGG 3 cut(s) 57, 1113, 1138
PspFI CCCAGC 3 cut(s) 563, 786, 1334
PspGI CCWGG 3 cut(s) 57, 1113, 1138
PspN4I GGNNCC 5 cut(s) 294, 342, 864, 931, 1340
PspPI GGNCC 3 cut(s) 293, 437, 1238
PsrI GAACNNNNNNTAC 4 cut(s) 837, 869, 954, 986
PstI CTGCAG 2 cut(s) 37, 1207
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 2 cut(s) 340, 1034
RsaI GTAC 1 cut(s) 39
RsaNI GTAC 1 cut(s) 38
RseI CAYNNNNRTG 1 cut(s) 743
SaqAI TTAA 4 cut(s) 225, 665, 804, 981
SatI GCNGC 9 cut(s) 182, 201, 379, 611, 1046, 1049, 1266, 1323, 1354
Sau3AI GATC 7 cut(s) 267, 340, 637, 680, 880, 1034, 1096
Sau96I GGNCC 3 cut(s) 293, 437, 1238
SchI GAGTC 2 cut(s) 71, 1095
ScrFI CCNGG 4 cut(s) 59, 186, 1115, 1140
SduI GDGCHC 2 cut(s) 191, 1025
SfaNI GCATC 5 cut(s) 643, 718, 954, 1321, 1396
SfcI CTRYAG 3 cut(s) 33, 624, 1203
Sfr274I CTCGAG 1 cut(s) 526
SlaI CTCGAG 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 743
SmlI CTYRAG 2 cut(s) 526, 1326
SmoI CTYRAG 2 cut(s) 526, 1326
SphI GCATGC 1 cut(s) 945
Sse9I AATT 9 cut(s) 93, 107, 121, 533, 541, 592, 838, 978, 982
SsiI CCGC 5 cut(s) 182, 265, 378, 720, 1353
SspMI CTAG 4 cut(s) 83, 126, 888, 1104
StyD4I CCNGG 4 cut(s) 57, 184, 1113, 1138
StyI CCWWGG 2 cut(s) 366, 1269
TaaI ACNGT 5 cut(s) 193, 1027, 1094, 1126, 1375
TaqI TCGA 6 cut(s) 20, 47, 140, 527, 1012, 1362
TasI AATT 9 cut(s) 93, 107, 121, 533, 541, 592, 838, 978, 982
TatI WGTACW 1 cut(s) 37
TauI GCSGC 3 cut(s) 184, 381, 1356
TfiI GAWTC 3 cut(s) 212, 326, 629
Tru1I TTAA 4 cut(s) 225, 665, 804, 981
Tru9I TTAA 4 cut(s) 225, 665, 804, 981
TscAI CASTG 4 cut(s) 196, 721, 1099, 1378
TseFI GTSAC 1 cut(s) 1246
TseI GCWGC 6 cut(s) 200, 610, 1045, 1048, 1265, 1322
Tsp45I GTSAC 1 cut(s) 1246
TspDTI ATGAA 6 cut(s) 492, 527, 621, 727, 765, 1205
TspGWI ACGGA 2 cut(s) 477, 1331
TspRI CASTG 4 cut(s) 196, 721, 1099, 1378
Van91I CCANNNNNTGG 1 cut(s) 515
VneI GTGCAC 1 cut(s) 1021
VspI ATTAAT 1 cut(s) 981
XapI RAATTY 2 cut(s) 121, 533
XceI RCATGY 3 cut(s) 555, 945, 977
XhoI CTCGAG 1 cut(s) 526
XmiI GTMKAC 1 cut(s) 970
XspI CTAG 4 cut(s) 83, 126, 888, 1104
Zsp2I ATGCAT 3 cut(s) 175, 947, 1411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.