MD08G1038000.v1.1

Exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006651
Physical Location & Seq
Forward (+)
2784176 .. 2800761
16586 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1038000.v1.1.491

Sequence Viewer

Length: 2646 bp
ATGGCGAAATCCAGCGCAGACGACCAGGAGCTTCGGCGGGCCTGCGAGGCCGCCATCGAAGGCACCAAGCAGAGCGTCGTCATGTCGATCCGCGTGGCCAAGAGCCGTGCGATCCGGGGTATAACGCACATGCTCGGCCGCGACATGGCCAAGGCTAGGGTCCTGGCTCTTTCCGTAAAATCCAAAGGCCAGAGGACTAAAGCCTTTCTTCGAGTCTTAAAATATTCCACCGGAGGAGTCCTTGAGCCTGCAAAGTTATACAAACTAAAGCATCTTTCAAAGGTGGAGGTTTTAACGAACGACCCTAGTGGGTGTACTTTTACCCTGGGCTTTGATAATCTTAGGAGTCAAAGTGTTGCTCCTCCTCAATGGACCATGCGAAATATTGATGACAGGAACCGCCTTTTACTTTGCATTTTGAACATATGCAAAGATGCTCTGGGTCAGCTTCCTAAAGTTGTTGGTATAGATGTCGTGGAGATGGCTCTTTGGGCTAAGGAAAATACACCAGCTGTCACTAGCCAAGGAAAGGAGCAAGAGGGACCTGCTGCATCTACAGTAACGGAAAGTGACTTGAAAGTAACTGTTGAAAAAGAACTTGTCTCCCAAGCTGAGGAAGAGGACATGGAGGCACTTTTGGGAACTTATGTTATGGGTATTGGTGAAGCAGAGGCATTTTCTGAAAGGTTGAAGAGAGAGCTTCTGGCTTTGGAAGCAGCAAATGTGCATGCGATTTTGGAAAGTGAACCCTTGATAGACGAGGTATTGCAAGGCCTTGAGGCAGCAACAAATTGTGTCGATGACATGGATGAATGGTTAGGCATTTTCAATGTGAAACTCAGACACATGAGAGAAGACATCGAATCGATAGAAACCCGCAATAACAAGTTGGAGGTGCAATCTGTAAATAATAGAGCTCTCATTGAGGAACTCGATAAGCTTCTTCTAGGTTTGCGCGTTCCTTCTGAGTATGCCGCATGTTTGACGGGAGGTTCATTTGATGAAGCACGTATGCTTCAAAACATAGAAGCGTGTGAGTGGTTGGCTGGTGCTTTACACAGTCTTGAAGCGCCAAACTTGGATCCTATATATGCAAATATACGGGCTGTTAAAGAGAAGCGAGAAGAACTTGAAAAACTGAAATCTACATTTGTCAGGAAAGCTTCTGAGTTCTTGCGAAATTATTTTGCTAGTTTGGTGGATTTTATGATAAGTGACAAGAGCTACTTTTCTCAGCGGGGCCAACTGAAAAGGCCTGATCATGCTGATCTACGGTACAAATGCAGAACATATGCTCGCCTTCTGCAACATTTAAAGAGTCTTGATAAGAATTGCATGGGCGCTTTGAGGAAAGCATATTGTAGCTCCCTCAACCTGCTTATTCGTCGTGAGGCTCGTGAATTTGCAAATGAGCTTCGTGCTAGTACAAAAGCATCAAGAAATCCAACCGTCTGGCTTGAAGCTTCTACTGGGTCTGGTCAAAATGCGAATGCTGCAGACACTTCTGCTGTTTCTGAAGCTTATTCCAAGATGCTTACAATTTTTATCCCACTCCTTGTAGATGAGAGTTCTTTCTTTGCACACTTTATGTGTTTTGAAGTTCCAGCACTTAATCCTCCTGGGGGTACTGCTAATGGAGATAAGTCTGATGACACCAATGACGATGATTTGGGAATCATGGACATTGATGACAATGACAGCAAAGCTGGTAAAAGTTCAGGAGAGCTTGCAGCATTAAATGAATCTCTTCAGGATTTACTCGATGGAATCCAGGAAGACTTTTATGCCGTTGTGGATTGGGCTTACAAGATTGATCCCTTACGCTGCATATCAATGCATGGGATTACAGAGCGCTATCTTTCTGGTCAGAAAGCTGATGCAGCAGGATTTGTGCGTCTCTTGCTTGGTGATCTGGAGTCGAGGGTTTCTATGCAGTTCAGTCGCTTTGTTGATGAAGCTTGCCACCAGATTGAAAGAAACGAGCGCAATGTTAGACAAATGGGTGTCTTATCATACATCCCAAGATTTGCTACTCTGGCAACACGAATGGAGCAGTACATCCAGGGACAATCAAGGGATTTGGTTGATCAAGCATACACAAAATTTGTTAGCATAATGTTTGTGACTTTGGAGAAAATTGCACAAACGGAGCCAAAATATTCTGATCTTTTTCTATTAGAGAACTATGCTGCATTTCAGAATAGTTTGTATGACCTAGCAAATGTTGTGCCCACCTTGGCCAAATTTTATCACCAGGCAAGTGAAGCTTATGAACAAGCTTGCACACGCCATAACAGCATGATCATATACACTCAATTTGAACGGCTTTTCCAGTTTGCTCGAAAAATAGAGGATTTGATGTTTACAATCGCACCAGAAGAGATCCCTTTCCAGCTTGGGTTGTCAAAGGCGGATCTGCGGAAGGTGTTAAAATCAAGCTTATACGGGCTTGACAAGTCCATCACTGCAATGTACAAGAAGTTGCAGAAAAACATGACTTCAGAAGAACTGCTGCCTTCTCTATGGGAAAAATGCAAGGAATTCCTTGAGAAGTATGAAACTTTTGCTCAACTCGTCGCCAAGATTTACCCGACAGAGACCTTTCTCACGGTAGCAGAACTGAGAGACCTTTTGGCTACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec3-PIP2_bind Sec3_C

Protein Analysis

882

Amino Acids

99.16

Weight (kDa)

5.41

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec3-PIP2_bind PF15277 50 - 144 1.1e-14 Exocyst complex component SEC3 N-terminal PIP2 binding PH
Sec3_CC PF09763 223 - 344 1.1e-25 Exocyst complex component Sec3, coiled-coil
Vps52_CC PF04129 255 - 392 3.8e-08 Vps52/Sac2 family, coiled-coil
Sec3_C-term PF20654 577 - 864 2.7e-25 Exocyst complex component Sec3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 553, 1383
AccB1I GGYRCC 1 cut(s) 62
AccII CGCG 3 cut(s) 93, 141, 957
AclWI GGATC 7 cut(s) 82, 106, 1076, 1089, 1808, 2377, 2421
AcoI YGGCCR 4 cut(s) 96, 136, 147, 2238
AcsI RAATTY 4 cut(s) 1400, 2102, 2243, 2540
AcuI CTGAAG 3 cut(s) 1536, 1733, 2484
AfaI GTAC 6 cut(s) 316, 1277, 1426, 1627, 2057, 2474
AfeI AGCGCT 1 cut(s) 1853
AfiI CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1622
AjnI CCWGG 7 cut(s) 24, 162, 324, 1618, 1770, 2061, 2253
AloI GAACNNNNNNTCC 2 cut(s) 2313, 2345
Alw21I GWGCWC 1 cut(s) 919
Alw26I GTCTC 4 cut(s) 607, 1901, 2591, 2619
AlwI GGATC 7 cut(s) 82, 106, 1076, 1089, 1808, 2377, 2421
Aor51HI AGCGCT 1 cut(s) 1853
ApeKI GCWGC 9 cut(s) 548, 716, 782, 1493, 1730, 1824, 1880, 2189, 2512
ApoI RAATTY 4 cut(s) 1400, 2102, 2243, 2540
Asp700I GAANNNNTTC 1 cut(s) 1746
AspLEI GCGC 6 cut(s) 17, 957, 1072, 1343, 1854, 1986
AspS9I GGNCC 5 cut(s) 39, 160, 372, 542, 1240
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 3 cut(s) 674, 1919, 2243
AvaII GGWCC 3 cut(s) 160, 372, 542
BaeGI GKGCMC 1 cut(s) 2232
BalI TGGCCA 3 cut(s) 98, 149, 2240
BamHI GGATCC 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 919
BauI CACGAG 1 cut(s) 1395
BbsI GAAGAC 2 cut(s) 861, 1782
Bbv12I GWGCWC 1 cut(s) 919
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 9 cut(s) 535, 728, 794, 1480, 1742, 1811, 1892, 2176, 2499
BccI CCATC 4 cut(s) 62, 475, 1757, 2468
BceAI ACGGC 3 cut(s) 90, 1772, 2339
BcgI CGANNNNNNTGC 2 cut(s) 1922, 1956
BciT130I CCWGG 7 cut(s) 26, 164, 326, 1620, 1772, 2063, 2255
BclI TGATCA 3 cut(s) 1258, 2086, 2301
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 4 cut(s) 607, 1901, 2591, 2619
BfaI CTAG 7 cut(s) 156, 306, 519, 947, 1191, 1422, 2216
BfmI CTRYAG 2 cut(s) 555, 1494
BfoI RGCGCY 3 cut(s) 1073, 1344, 1855
BfuAI ACCTGC 2 cut(s) 553, 1383
BglI GCCNNNNNGGC 1 cut(s) 47
Bme1390I CCNGG 8 cut(s) 26, 116, 164, 326, 1620, 1772, 2063, 2255
Bme18I GGWCC 3 cut(s) 160, 372, 542
BmgT120I GGNCC 5 cut(s) 39, 160, 372, 542, 1240
BmiI GGNNCC 7 cut(s) 64, 161, 398, 543, 1083, 1241, 2151
BmrFI CCNGG 8 cut(s) 26, 116, 164, 326, 1620, 1772, 2063, 2255
BmrI ACTGGG 1 cut(s) 1479
BmsI GCATC 6 cut(s) 280, 424, 560, 1442, 1521, 1867
BmuI ACTGGG 1 cut(s) 1479
BpiI GAAGAC 2 cut(s) 861, 1782
BpmI CTGGAG 1 cut(s) 1934
Bpu10I CCTNAGC 2 cut(s) 495, 612
BpuEI CTTGAG 3 cut(s) 263, 797, 2567
BpuMI CCSGG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 866
BsaAI YACGTR 1 cut(s) 1010
BsaI GGTCTC 2 cut(s) 2591, 2619
BsaJI CCNNGG 8 cut(s) 115, 150, 324, 325, 523, 1619, 2062, 2235
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1622
Bse1I ACTGG 2 cut(s) 1474, 2332
Bse3DI GCAATG 2 cut(s) 1993, 2475
BseBI CCWGG 7 cut(s) 26, 164, 326, 1620, 1772, 2063, 2255
BseCI ATCGAT 1 cut(s) 866
BseDI CCNNGG 8 cut(s) 115, 150, 324, 325, 523, 1619, 2062, 2235
BseGI GGATG 3 cut(s) 814, 2016, 2058
BseLI CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1622
BseMI GCAATG 2 cut(s) 1993, 2475
BseMII CTCAG 6 cut(s) 603, 853, 957, 1158, 1247, 2612
BseNI ACTGG 2 cut(s) 1474, 2332
BseRI GAGGAG 3 cut(s) 249, 351, 354
BseSI GKGCMC 1 cut(s) 2232
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 9 cut(s) 535, 728, 794, 1480, 1742, 1811, 1892, 2176, 2499
Bsh1236I CGCG 3 cut(s) 93, 141, 957
Bsh1285I CGRYCG 1 cut(s) 139
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 866
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 919
BsiSI CCGG 2 cut(s) 115, 231
BslFI GGGAC 2 cut(s) 555, 2079
BslI CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1622
BsmAI GTCTC 4 cut(s) 607, 1901, 2591, 2619
BsmBI CGTCTC 1 cut(s) 1901
BsmFI GGGAC 2 cut(s) 555, 2079
BsmI GAATGC 1 cut(s) 1495
Bso31I GGTCTC 2 cut(s) 2591, 2619
Bsp1286I GDGCHC 2 cut(s) 919, 2232
Bsp1407I TGTACA 1 cut(s) 2472
BspCNI CTCAG 6 cut(s) 604, 852, 958, 1159, 1246, 2613
BspDI ATCGAT 1 cut(s) 866
BspFNI CGCG 3 cut(s) 93, 141, 957
BspLI GGNNCC 7 cut(s) 64, 161, 398, 543, 1083, 1241, 2151
BspMAI CTGCAG 1 cut(s) 1498
BspMI ACCTGC 2 cut(s) 553, 1383
BspPI GGATC 7 cut(s) 82, 106, 1076, 1089, 1808, 2377, 2421
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 2 cut(s) 2591, 2619
BsrDI GCAATG 2 cut(s) 1993, 2475
BsrGI TGTACA 1 cut(s) 2472
BsrI ACTGG 2 cut(s) 1474, 2332
BssECI CCNNGG 8 cut(s) 115, 150, 324, 325, 523, 1619, 2062, 2235
BssSI CACGAG 1 cut(s) 1395
BssT1I CCWWGG 3 cut(s) 150, 523, 2235
Bst2BI CACGAG 1 cut(s) 1395
Bst2UI CCWGG 7 cut(s) 26, 164, 326, 1620, 1772, 2063, 2255
Bst4CI ACNGT 6 cut(s) 559, 586, 1061, 1275, 1450, 2611
Bst6I CTCTTC 4 cut(s) 612, 686, 1752, 2373
BstAUI TGTACA 1 cut(s) 2472
BstBAI YACGTR 1 cut(s) 1010
BstC8I GCNNGC 8 cut(s) 39, 43, 249, 729, 1297, 1728, 1960, 2281
BstDEI CTNAG 8 cut(s) 341, 495, 612, 839, 966, 1167, 1233, 2621
BstF5I GGATG 3 cut(s) 814, 2016, 2058
BstFNI CGCG 3 cut(s) 93, 141, 957
BstH2I RGCGCY 3 cut(s) 1073, 1344, 1855
BstHHI GCGC 6 cut(s) 17, 957, 1072, 1343, 1854, 1986
BstMAI GTCTC 4 cut(s) 607, 1901, 2591, 2619
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 9 cut(s) 47, 491, 713, 1493, 1880, 1900, 2036, 2264, 2295
BstNI CCWGG 7 cut(s) 26, 164, 326, 1620, 1772, 2063, 2255
BstNSI RCATGY 3 cut(s) 133, 731, 981
BstSCI CCNGG 8 cut(s) 24, 114, 162, 324, 1618, 1770, 2061, 2253
BstSFI CTRYAG 2 cut(s) 555, 1494
BstSLI GKGCMC 1 cut(s) 2232
BstUI CGCG 3 cut(s) 93, 141, 957
BstV1I GCAGC 9 cut(s) 535, 728, 794, 1480, 1742, 1811, 1892, 2176, 2499
BstV2I GAAGAC 2 cut(s) 861, 1782
BstX2I RGATCY 3 cut(s) 1081, 2382, 2413
BstXI CCANNNNNNTGG 1 cut(s) 1452
BstYI RGATCY 3 cut(s) 1081, 2382, 2413
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 866
BsuTUI ATCGAT 1 cut(s) 866
BtsCI GGATG 3 cut(s) 814, 2016, 2058
BtsI GCAGTG 1 cut(s) 2463
BtsIMutI CAGTG 1 cut(s) 2463
BveI ACCTGC 2 cut(s) 553, 1383
Cac8I GCNNGC 8 cut(s) 39, 43, 249, 729, 1297, 1728, 1960, 2281
CfoI GCGC 6 cut(s) 17, 957, 1072, 1343, 1854, 1986
Cfr13I GGNCC 5 cut(s) 39, 160, 372, 542, 1240
ClaI ATCGAT 1 cut(s) 866
CseI GACGC 2 cut(s) 64, 1883
Csp6I GTAC 6 cut(s) 315, 1276, 1425, 1626, 2056, 2473
CviQI GTAC 6 cut(s) 315, 1276, 1425, 1626, 2056, 2473
DdeI CTNAG 8 cut(s) 341, 495, 612, 839, 966, 1167, 1233, 2621
DraI TTTAAA 1 cut(s) 1314
EaeI YGGCCR 4 cut(s) 96, 136, 147, 2238
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 4 cut(s) 612, 686, 1752, 2373
EarI CTCTTC 4 cut(s) 612, 686, 1752, 2373
EciI GGCGGA 1 cut(s) 2426
Ecl136II GAGCTC 1 cut(s) 917
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 3 cut(s) 150, 523, 2235
Eco147I AGGCCT 2 cut(s) 774, 1255
Eco24I GRGCYC 1 cut(s) 919
Eco31I GGTCTC 2 cut(s) 2591, 2619
Eco47I GGWCC 3 cut(s) 160, 372, 542
Eco47III AGCGCT 1 cut(s) 1853
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 917
Eco57I CTGAAG 3 cut(s) 1536, 1733, 2484
EcoICRI GAGCTC 1 cut(s) 917
EcoO109I RGGNCCY 2 cut(s) 160, 542
EcoRI GAATTC 1 cut(s) 2540
EcoRII CCWGG 7 cut(s) 24, 162, 324, 1618, 1770, 2061, 2253
EcoT14I CCWWGG 3 cut(s) 150, 523, 2235
EcoT22I ATGCAT 1 cut(s) 1839
EcoT38I GRGCYC 1 cut(s) 919
ErhI CCWWGG 3 cut(s) 150, 523, 2235
Esp3I CGTCTC 1 cut(s) 1901
FalI AAGNNNNNCTT 6 cut(s) 192, 224, 1211, 1243, 2251, 2283
FaqI GGGAC 2 cut(s) 555, 2079
FauI CCCGC 3 cut(s) 30, 884, 1230
FauNDI CATATG 2 cut(s) 425, 1291
FbaI TGATCA 3 cut(s) 1258, 2086, 2301
FokI GGATG 3 cut(s) 821, 2003, 2045
FriOI GRGCYC 1 cut(s) 919
FspBI CTAG 7 cut(s) 156, 306, 519, 947, 1191, 1422, 2216
GlaI GCGC 6 cut(s) 16, 956, 1071, 1342, 1853, 1985
GsuI CTGGAG 1 cut(s) 1934
HaeII RGCGCY 3 cut(s) 1073, 1344, 1855
HapII CCGG 2 cut(s) 115, 231
HgaI GACGC 2 cut(s) 64, 1883
HhaI GCGC 6 cut(s) 17, 957, 1072, 1343, 1854, 1986
Hin6I GCGC 6 cut(s) 15, 955, 1070, 1341, 1852, 1984
HinP1I GCGC 6 cut(s) 15, 955, 1070, 1341, 1852, 1984
HindIII AAGCTT 8 cut(s) 938, 1161, 1461, 1518, 1956, 2265, 2277, 2437
HinfI GANTC 9 cut(s) 213, 237, 346, 863, 1318, 1674, 1742, 1767, 1916
HpaII CCGG 2 cut(s) 115, 231
HphI GGTGA 3 cut(s) 674, 1919, 2243
Hpy166II GTNNAC 3 cut(s) 315, 746, 2364
Hpy188III TCNNGA 9 cut(s) 1064, 1156, 1322, 1388, 1397, 1437, 1719, 1751, 1913
Hpy8I GTNNAC 3 cut(s) 315, 746, 2364
Hpy99I CGWCG 3 cut(s) 80, 1389, 2579
HpyAV CCTTC 5 cut(s) 53, 972, 1310, 2416, 2526
HpyCH4III ACNGT 6 cut(s) 559, 586, 1061, 1275, 1450, 2611
HpyCH4IV ACGT 1 cut(s) 1009
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 491, 713, 1493, 1880, 1900, 2036, 2264, 2295
HpyF3I CTNAG 8 cut(s) 341, 495, 612, 839, 966, 1167, 1233, 2621
HpySE526I ACGT 1 cut(s) 1009
HspAI GCGC 6 cut(s) 15, 955, 1070, 1341, 1852, 1984
Ksp22I TGATCA 3 cut(s) 1258, 2086, 2301
LmnI GCTCC 6 cut(s) 28, 364, 532, 1370, 2050, 2149
Lsp1109I GCAGC 9 cut(s) 535, 728, 794, 1480, 1742, 1811, 1892, 2176, 2499
LweI GCATC 6 cut(s) 280, 424, 560, 1442, 1521, 1867
MaeI CTAG 7 cut(s) 156, 306, 519, 947, 1191, 1422, 2216
MaeII ACGT 1 cut(s) 1009
MaeIII GTNAC 6 cut(s) 514, 559, 569, 580, 1214, 2122
MflI RGATCY 3 cut(s) 1081, 2382, 2413
MhlI GDGCHC 2 cut(s) 919, 2232
MlsI TGGCCA 3 cut(s) 98, 149, 2240
MluNI TGGCCA 3 cut(s) 98, 149, 2240
MlyI GAGTC 5 cut(s) 222, 246, 355, 1327, 1925
MmeI TCCRAC 2 cut(s) 870, 1469
Mox20I TGGCCA 3 cut(s) 98, 149, 2240
Mph1103I ATGCAT 1 cut(s) 1839
MroXI GAANNNNTTC 1 cut(s) 1746
MscI TGGCCA 3 cut(s) 98, 149, 2240
MseI TTAA 7 cut(s) 218, 293, 1110, 1313, 1611, 1736, 2429
MslI CAYNNNNRTG 2 cut(s) 1832, 2468
Msp20I TGGCCA 3 cut(s) 98, 149, 2240
MspA1I CMGCKG 2 cut(s) 512, 1237
MspI CCGG 2 cut(s) 115, 231
MspR9I CCNGG 8 cut(s) 26, 116, 164, 326, 1620, 1772, 2063, 2255
Mva1269I GAATGC 1 cut(s) 1495
MvaI CCWGG 7 cut(s) 26, 164, 326, 1620, 1772, 2063, 2255
MvnI CGCG 3 cut(s) 93, 141, 957
MwoI GCNNNNNNNGC 9 cut(s) 47, 491, 713, 1493, 1880, 1900, 2036, 2264, 2295
NciI CCSGG 1 cut(s) 116
NdeI CATATG 2 cut(s) 425, 1291
NlaIV GGNNCC 7 cut(s) 64, 161, 398, 543, 1083, 1241, 2151
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 4 cut(s) 514, 569, 1214, 2122
NsiI ATGCAT 1 cut(s) 1839
NspI RCATGY 3 cut(s) 133, 731, 981
PaeI GCATGC 1 cut(s) 731
PasI CCCWGGG 1 cut(s) 325
PceI AGGCCT 2 cut(s) 774, 1255
PcsI WCGNNNNNNNCGW 1 cut(s) 1393
PctI GAATGC 1 cut(s) 1495
PdmI GAANNNNTTC 1 cut(s) 1746
PfeI GAWTC 4 cut(s) 863, 1674, 1742, 1767
PfoI TCCNGGA 1 cut(s) 1770
PleI GAGTC 5 cut(s) 221, 245, 354, 1326, 1924
PpsI GAGTC 5 cut(s) 221, 245, 354, 1326, 1924
Ppu21I YACGTR 1 cut(s) 1010
PpuMI RGGWCCY 2 cut(s) 160, 542
Psp124BI GAGCTC 1 cut(s) 919
Psp5II RGGWCCY 2 cut(s) 160, 542
Psp6I CCWGG 7 cut(s) 24, 162, 324, 1618, 1770, 2061, 2253
PspGI CCWGG 7 cut(s) 24, 162, 324, 1618, 1770, 2061, 2253
PspN4I GGNNCC 7 cut(s) 64, 161, 398, 543, 1083, 1241, 2151
PspPI GGNCC 5 cut(s) 39, 160, 372, 542, 1240
PspPPI RGGWCCY 2 cut(s) 160, 542
PstI CTGCAG 1 cut(s) 1498
PsuI RGATCY 3 cut(s) 1081, 2382, 2413
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 6 cut(s) 316, 1277, 1426, 1627, 2057, 2474
RsaNI GTAC 6 cut(s) 315, 1276, 1425, 1626, 2056, 2473
RseI CAYNNNNRTG 2 cut(s) 1832, 2468
SacI GAGCTC 1 cut(s) 919
SaqAI TTAA 7 cut(s) 218, 293, 1110, 1313, 1611, 1736, 2429
Sau96I GGNCC 5 cut(s) 39, 160, 372, 542, 1240
SchI GAGTC 5 cut(s) 222, 246, 355, 1327, 1925
ScrFI CCNGG 8 cut(s) 26, 116, 164, 326, 1620, 1772, 2063, 2255
SduI GDGCHC 2 cut(s) 919, 2232
SfaNI GCATC 6 cut(s) 280, 424, 560, 1442, 1521, 1867
SfcI CTRYAG 2 cut(s) 555, 1494
SfiI GGCCNNNNNGGCC 1 cut(s) 47
SinI GGWCC 3 cut(s) 160, 372, 542
SmiMI CAYNNNNRTG 2 cut(s) 1832, 2468
SmlI CTYRAG 3 cut(s) 242, 776, 2546
SmoI CTYRAG 3 cut(s) 242, 776, 2546
SphI GCATGC 1 cut(s) 731
SseBI AGGCCT 2 cut(s) 774, 1255
SspI AATATT 3 cut(s) 224, 385, 2159
SspMI CTAG 7 cut(s) 156, 306, 519, 947, 1191, 1422, 2216
SstI GAGCTC 1 cut(s) 919
StuI AGGCCT 2 cut(s) 774, 1255
StyD4I CCNGG 8 cut(s) 24, 114, 162, 324, 1618, 1770, 2061, 2253
StyI CCWWGG 3 cut(s) 150, 523, 2235
TaaI ACNGT 6 cut(s) 559, 586, 1061, 1275, 1450, 2611
TaiI ACGT 1 cut(s) 1012
TatI WGTACW 4 cut(s) 314, 1424, 2055, 2472
TauI GCSGC 3 cut(s) 53, 141, 977
TfiI GAWTC 4 cut(s) 863, 1674, 1742, 1767
Tru1I TTAA 7 cut(s) 218, 293, 1110, 1313, 1611, 1736, 2429
Tru9I TTAA 7 cut(s) 218, 293, 1110, 1313, 1611, 1736, 2429
TscAI CASTG 1 cut(s) 2470
TseFI GTSAC 4 cut(s) 514, 569, 1214, 2122
TseI GCWGC 9 cut(s) 548, 716, 782, 1493, 1730, 1824, 1880, 2189, 2512
Tsp45I GTSAC 4 cut(s) 514, 569, 1214, 2122
TspDTI ATGAA 7 cut(s) 825, 984, 1017, 1755, 1968, 2286, 2571
TspGWI ACGGA 3 cut(s) 163, 578, 2162
TspRI CASTG 1 cut(s) 2470
VpaK11BI GGWCC 3 cut(s) 160, 372, 542
XapI RAATTY 4 cut(s) 1400, 2102, 2243, 2540
XceI RCATGY 3 cut(s) 133, 731, 981
XmnI GAANNNNTTC 1 cut(s) 1746
XspI CTAG 7 cut(s) 156, 306, 519, 947, 1191, 1422, 2216
Zsp2I ATGCAT 1 cut(s) 1839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.