Rh6CG422100

Exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006651
Physical Location & Seq
Reverse (-)
60517667 .. 60527965
10299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG422100.1

Sequence Viewer

Length: 2667 bp
ATGGCGAAATCCAGCGCCGACGACCAGGAGCTCCGGCGAGCCTGCGAGGCCGCAATCGAAGGCACCAAGCAGTCCGTGGTCATGTCGATCCGTGTCGCCAAGAGCCGGGGCATGTGGGGGAAGACTCACAAGCTCGGCCGCGACATGGCCAAACCTAGGGTTCTTGCCCTTTCCGTAAAAAACAAAGGCCAGCGGACTAAAGCCTTTCTTCGAGTCTTAAAATATTCCACTGGAGGAGTTCTAGAGCCTGCAAAGTTATACAAGCTAAAGCATCTTTCGAAGGTGGAGGTTTTAACAAGCGACCCTAGTGGATGTACTTTTACTCTGGGTTTTGATAATCTTAGGAGCCAGAGTGTTGCTCCTCCTCAATGGACCATGCGAAACATTGATGACAGGAACCGCCTTTTACTATGTATTTTAAACATATGCAAAGATGCACTGGAACAGCTGCCTAAAGTTGTTGGTATAGATGTCGTCGAGATGGCTCTTTGGGCTAAGGAAAATACACCAGCAATTTCTAGTCAAAAAAACCAGCAAGAGGGAGAGGGACCTGCTGCAGCAACAGTGACTGAACGTGACTTGAAAGTAACTGTTGAGAAAGAACTTGTCTCACAAGCTGAGGAAGAGGACATGGAGGCACTTTTGGGAACTTATGTCATGGGTATTGGTGAAGCAGAGGCATTTTCTGAAAGGTTGAAAAGAGAGCTTCTGGCTCTGGAAGCAGCAAATGTGCATGCCATTTTGGAAAGTGAACCTTTGATTGATGAGGTTTTGCAAGGAATTGAATCAGCAACAAATTGTGTTGATGACATGGATGAATGGTTAAGCATCTTCAATGTGAAACTCAGACACATGAGAGAAGACATTGAGTCGATAGAAACCCGCAATAACAAGTTGGAAACGCAATCTGTAAATAATAAAGCACTCATCGAGGAGCTTGACAAGCTTCTTAAAGGCTTGCATGTCCCTACGGAGTATGCAACATGTTTGACTGGAGGTTCATTTGACGAGGCACGTATGCTTCAAAACATAGAAGCATGTGAGTGGTTAGCTGGTGCTCTACGCAGTCTTGAAGTACCCCATTTGGACCCTATCTATGCAAACATGCGAGCTGTTAAAGAGAAGCGGGCAGAACTTGAAAAATTAAAAGCTACATTTGTGAGAAGAGCTTCTGAGTTTTTGCGAAATTACTTTGCTAGTTTGGTGGATTTCATGATAAGTGACAAGAGCTACTTTTCTCAGCGGGGGCAACTGAAAAGGCCTGATCATGCTGATCTACGGTATAAATGCAGGACATATGCTCGCCTTCTGCAACATTTAAAGACTCTTGATAAGAATTGCCTTGGGCCACTGAGAAAAGCATATTGTAGCTCCCTCAACTTGCTTCTTCGTCGGGAGGCTCGTGAATTTGCAAATGAGCTTCGTGCTAGTACAAAGGCATCAAAAAATCCAACTGTCTGGCTTGAAGCTTCTACTGGTTCTGGTCAAAATATGAATGCTGCAGACACTTCTACTGTTTCTGAAGCTTATTCCAAGATGCTAACAATTTTTATCCCACTCCTTGTAGATGAGAGTTCTTTTTTCGCACACTTTATGTGCTTTGAAGTTCCTGCACTTGTTCCTCCAGGGGGTACTGCCAATGGAGATAAATCTGAATATGATGATGATGACCCGAATGATGACGATTTGGGAATCATGGACATTGATGACAATGACAGTAAAGCTGGTAAACAAACGGGAGAGCTTGCAGCATTGAATGAATCTCTTCAGGATTTACTAGATGGAATCCAAGAAGACTTTTATGCTGTGGTGGACTGGGCATACAAGATTGATCCTTTACGCTGCATATCAATGCATGGGATTACAGAGCGTTATCTTTCGGGTCAGAAAGCTGATGCAGCAGGATTTGTGCGTCTCCTGCTGGGCGATCTGGAGTCAAGAGTATCAATGCAATTTAGTCGTTTCGTTGATGAAGCATGCCACCAGATTGAAAGAAATGAGCGCAATGTTAGGCAAATGGGTGTCTTGTCATACATCCCAAGATTTGCAACTTTGGCAACGCGAATGGAGCAGTATATCCAGGGACAATCTAGGGATTTAGTTGATCAAGCGTACACAAAATTTGTTAGCATAATGTTTGTGACTTTAGAGAAGATTGCACAAACAGATCCAAAATATTCTGATCTTTTCCTCTTAGAGAACTATGCTGCATTTCAGAATAGTTTGTATGACCTAGCAAATGTTGTTCCTACATTGGCCAAATTTTATCACCAAGCAAGTGAAGCTTATGAACAGGCTTGCACACGCCATATCAGTATGATCATATACGCTCAATTTGAACGGCTTTTCCAGTTCGCTAAAAAAATAGAGGATTTGATGTATACAATCCCTCCTGAAGAGATCCCTTTCCAGCTTGGGTTGTCAAAGGTGGATTTGCGGAAGATGTTAAAATATAGTTTATCTGGGCTTGACAAGTCCATCAGTGCAATGTACAAGAAGTTGCAGAAAAACATGACATCAGAAGAATTGCTGCCTTCCTTATGGGATAAATGCAAGAAGGAGTTCCTTGACAAGTATGAAAGTTTTGCTCAACTTGTCAACAAAATCTACCCCACAGAGACCATTCCTACTGTAACAGAGATGAGAGAACTTTTGGCTAACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec3-PIP2_bind Sec3_C

Protein Analysis

888

Amino Acids

100.52

Weight (kDa)

5.49

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec3-PIP2_bind PF15277 50 - 144 4.1e-15 Exocyst complex component SEC3 N-terminal PIP2 binding PH
Sec3_CC PF09763 225 - 346 3.5e-27 Exocyst complex component Sec3, coiled-coil
Vps52_CC PF04129 255 - 394 3e-09 Vps52/Sac2 family, coiled-coil
Sec3_C-term PF20654 584 - 871 3.4e-29 Exocyst complex component Sec3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 2381
AccII CGCG 2 cut(s) 141, 2062
AciI CCGC 8 cut(s) 51, 139, 193, 400, 883, 1126, 1243, 2437
AclWI GGATC 4 cut(s) 82, 1826, 2162, 2395
AcoI YGGCCR 3 cut(s) 136, 147, 2256
AcsI RAATTY 3 cut(s) 1406, 2120, 2261
AcuI CTGAAG 3 cut(s) 1542, 1751, 2415
AfaI GTAC 6 cut(s) 316, 1077, 1432, 1633, 2114, 2492
AfiI CCNNNNNNNGG 5 cut(s) 105, 145, 156, 538, 1628
AflIII ACRYGT 2 cut(s) 983, 2661
AhdI GACNNNNNGTC 1 cut(s) 868
AjnI CCWGG 3 cut(s) 24, 1624, 2079
AloI GAACNNNNNNTCC 2 cut(s) 2331, 2363
Alw21I GWGCWC 2 cut(s) 33, 1060
Alw26I GTCTC 3 cut(s) 613, 1919, 2612
AlwI GGATC 4 cut(s) 82, 1826, 2162, 2395
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 7 cut(s) 48, 136, 147, 187, 1259, 1346, 2256
ApoI RAATTY 3 cut(s) 1406, 2120, 2261
Asp700I GAANNNNTTC 3 cut(s) 1168, 1764, 2561
AspA2I CCTAGG 1 cut(s) 155
AspLEI GCGC 2 cut(s) 17, 2004
AspS9I GGNCC 4 cut(s) 372, 548, 1087, 1346
AsuC2I CCSGG 1 cut(s) 107
AsuHPI GGTGA 2 cut(s) 680, 2261
AsuII TTCGAA 1 cut(s) 278
AvaII GGWCC 3 cut(s) 372, 548, 1087
AvrII CCTAGG 1 cut(s) 155
BalI TGGCCA 2 cut(s) 149, 2258
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 1 cut(s) 33
BauI CACGAG 1 cut(s) 1401
BbsI GAAGAC 3 cut(s) 128, 867, 1800
Bbv12I GWGCWC 2 cut(s) 33, 1060
BbvCI CCTCAGC 1 cut(s) 618
BccI CCATC 3 cut(s) 475, 1775, 2486
BceAI ACGGC 1 cut(s) 2357
BcgI CGANNNNNNTGC 2 cut(s) 1940, 1974
BciT130I CCWGG 3 cut(s) 26, 1626, 2081
BclI TGATCA 3 cut(s) 1264, 2104, 2319
BcnI CCSGG 1 cut(s) 107
BcoDI GTCTC 3 cut(s) 613, 1919, 2612
BfaI CTAG 9 cut(s) 156, 242, 306, 519, 1197, 1428, 1778, 2091, 2234
BfmI CTRYAG 2 cut(s) 555, 1500
BfoI RGCGCY 1 cut(s) 18
BfuAI ACCTGC 1 cut(s) 559
BglI GCCNNNNNGGC 1 cut(s) 47
BlnI CCTAGG 1 cut(s) 155
Bme1390I CCNGG 4 cut(s) 26, 107, 1626, 2081
Bme18I GGWCC 3 cut(s) 372, 548, 1087
BmeRI GACNNNNNGTC 1 cut(s) 868
BmgT120I GGNCC 4 cut(s) 372, 548, 1087, 1346
BmiI GGNNCC 5 cut(s) 64, 347, 398, 549, 1089
BmrFI CCNGG 4 cut(s) 26, 107, 1626, 2081
BmrI ACTGGG 1 cut(s) 1825
BmsI GCATC 6 cut(s) 280, 424, 837, 1448, 1527, 1885
BmuI ACTGGG 1 cut(s) 1825
BpiI GAAGAC 3 cut(s) 128, 867, 1800
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 4 cut(s) 252, 1014, 1608, 1952
Bpu10I CCTNAGC 2 cut(s) 495, 618
Bpu14I TTCGAA 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 1016
BsaI GGTCTC 1 cut(s) 2612
BsaJI CCNNGG 6 cut(s) 75, 106, 155, 1342, 1625, 2080
BsaXI ACNNNNNCTCC 4 cut(s) 337, 367, 2374, 2404
Bsc4I CCNNNNNNNGG 5 cut(s) 105, 145, 156, 538, 1628
Bse1I ACTGG 6 cut(s) 235, 444, 997, 1480, 1820, 2350
Bse3DI GCAATG 2 cut(s) 2011, 2493
BseBI CCWGG 3 cut(s) 26, 1626, 2081
BseDI CCNNGG 6 cut(s) 75, 106, 155, 1342, 1625, 2080
BseGI GGATG 3 cut(s) 317, 820, 2034
BseLI CCNNNNNNNGG 5 cut(s) 105, 145, 156, 538, 1628
BseMI GCAATG 2 cut(s) 2011, 2493
BseMII CTCAG 5 cut(s) 609, 859, 1164, 1253, 1343
BseNI ACTGG 6 cut(s) 235, 444, 997, 1480, 1820, 2350
BseRI GAGGAG 4 cut(s) 249, 351, 354, 947
BseX3I CGGCCG 1 cut(s) 136
BseYI CCCAGC 1 cut(s) 1921
BsgI GTGCAG 1 cut(s) 1596
Bsh1236I CGCG 2 cut(s) 141, 2062
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 7 cut(s) 50, 138, 149, 189, 1261, 1348, 2258
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 2 cut(s) 33, 1060
BsiSI CCGG 2 cut(s) 34, 106
BslFI GGGAC 3 cut(s) 561, 950, 2097
BslI CCNNNNNNNGG 5 cut(s) 105, 145, 156, 538, 1628
BsmAI GTCTC 3 cut(s) 613, 1919, 2612
BsmBI CGTCTC 1 cut(s) 1919
BsmFI GGGAC 3 cut(s) 561, 950, 2097
BsmI GAATGC 1 cut(s) 1501
BsnI GGCC 7 cut(s) 50, 138, 149, 189, 1261, 1348, 2258
Bso31I GGTCTC 1 cut(s) 2612
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 2 cut(s) 33, 1060
Bsp1407I TGTACA 1 cut(s) 2490
BspACI CCGC 8 cut(s) 51, 139, 193, 400, 883, 1126, 1243, 2437
BspANI GGCC 7 cut(s) 50, 138, 149, 189, 1261, 1348, 2258
BspCNI CTCAG 5 cut(s) 610, 858, 1165, 1252, 1344
BspFNI CGCG 2 cut(s) 141, 2062
BspHI TCATGA 1 cut(s) 1212
BspLI GGNNCC 5 cut(s) 64, 347, 398, 549, 1089
BspMAI CTGCAG 2 cut(s) 559, 1504
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 4 cut(s) 82, 1826, 2162, 2395
BspQI GCTCTTC 1 cut(s) 1159
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 2612
BsrDI GCAATG 2 cut(s) 2011, 2493
BsrGI TGTACA 1 cut(s) 2490
BsrI ACTGG 6 cut(s) 235, 444, 997, 1480, 1820, 2350
BssECI CCNNGG 6 cut(s) 75, 106, 155, 1342, 1625, 2080
BssNAI GTATAC 1 cut(s) 2382
BssSI CACGAG 1 cut(s) 1401
BssT1I CCWWGG 2 cut(s) 155, 1342
Bst1107I GTATAC 1 cut(s) 2382
Bst2BI CACGAG 1 cut(s) 1401
Bst2UI CCWGG 3 cut(s) 26, 1626, 2081
Bst4CI ACNGT 7 cut(s) 565, 592, 1281, 1456, 1516, 1718, 2632
Bst6I CTCTTC 4 cut(s) 618, 1159, 1770, 2391
BstAUI TGTACA 1 cut(s) 2490
BstBAI YACGTR 1 cut(s) 1016
BstBI TTCGAA 1 cut(s) 278
BstDEI CTNAG 8 cut(s) 341, 495, 618, 845, 1173, 1239, 1352, 2194
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 3 cut(s) 317, 820, 2034
BstFNI CGCG 2 cut(s) 141, 2062
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 2 cut(s) 17, 2004
BstMAI GTCTC 3 cut(s) 613, 1919, 2612
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 9 cut(s) 47, 491, 719, 943, 1898, 1918, 2054, 2068, 2282
BstNI CCWGG 3 cut(s) 26, 1626, 2081
BstNSI RCATGY 8 cut(s) 115, 737, 965, 987, 1041, 1108, 1980, 2665
BstSCI CCNGG 4 cut(s) 24, 105, 1624, 2079
BstSFI CTRYAG 2 cut(s) 555, 1500
BstUI CGCG 2 cut(s) 141, 2062
BstV2I GAAGAC 3 cut(s) 128, 867, 1800
BstX2I RGATCY 2 cut(s) 2167, 2400
BstXI CCANNNNNNTGG 1 cut(s) 1458
BstYI RGATCY 2 cut(s) 2167, 2400
BstZ17I GTATAC 1 cut(s) 2382
BstZI CGGCCG 1 cut(s) 136
BsuRI GGCC 7 cut(s) 50, 138, 149, 189, 1261, 1348, 2258
BtgI CCRYGG 1 cut(s) 75
BtsCI GGATG 3 cut(s) 317, 820, 2034
BtsIMutI CAGTG 5 cut(s) 228, 437, 570, 1349, 2488
BveI ACCTGC 1 cut(s) 559
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 1212
CfoI GCGC 2 cut(s) 17, 2004
Cfr13I GGNCC 4 cut(s) 372, 548, 1087, 1346
CseI GACGC 1 cut(s) 1901
Csp6I GTAC 6 cut(s) 315, 1076, 1431, 1632, 2113, 2491
CviQI GTAC 6 cut(s) 315, 1076, 1431, 1632, 2113, 2491
DdeI CTNAG 8 cut(s) 341, 495, 618, 845, 1173, 1239, 1352, 2194
DraI TTTAAA 2 cut(s) 420, 1320
DriI GACNNNNNGTC 1 cut(s) 868
EaeI YGGCCR 3 cut(s) 136, 147, 2256
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 4 cut(s) 618, 1159, 1770, 2391
Eam1105I GACNNNNNGTC 1 cut(s) 868
EarI CTCTTC 4 cut(s) 618, 1159, 1770, 2391
Ecl136II GAGCTC 1 cut(s) 31
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 155, 1342
Eco147I AGGCCT 1 cut(s) 1261
Eco24I GRGCYC 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 2612
Eco47I GGWCC 3 cut(s) 372, 548, 1087
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 3 cut(s) 1542, 1751, 2415
EcoICRI GAGCTC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 24, 1624, 2079
EcoT14I CCWWGG 2 cut(s) 155, 1342
EcoT22I ATGCAT 1 cut(s) 1857
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 155, 1342
Esp3I CGTCTC 1 cut(s) 1919
FalI AAGNNNNNCTT 8 cut(s) 192, 224, 739, 771, 1217, 1249, 2269, 2301
FaqI GGGAC 3 cut(s) 561, 950, 2097
FauI CCCGC 3 cut(s) 890, 1119, 1236
FauNDI CATATG 2 cut(s) 425, 1297
FbaI TGATCA 3 cut(s) 1264, 2104, 2319
FblI GTMKAC 1 cut(s) 2381
FokI GGATG 3 cut(s) 324, 827, 2021
FriOI GRGCYC 1 cut(s) 33
FspBI CTAG 9 cut(s) 156, 242, 306, 519, 1197, 1428, 1778, 2091, 2234
GlaI GCGC 2 cut(s) 16, 2003
GsaI CCCAGC 1 cut(s) 1925
GsuI CTGGAG 4 cut(s) 252, 1014, 1608, 1952
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 7 cut(s) 50, 138, 149, 189, 1261, 1348, 2258
HapII CCGG 2 cut(s) 34, 106
HgaI GACGC 1 cut(s) 1901
HhaI GCGC 2 cut(s) 17, 2004
Hin6I GCGC 2 cut(s) 15, 2002
HinP1I GCGC 2 cut(s) 15, 2002
HincII GTYRAC 1 cut(s) 2599
HindII GTYRAC 1 cut(s) 2599
HindIII AAGCTT 4 cut(s) 944, 1467, 1524, 2283
HinfI GANTC 9 cut(s) 124, 213, 785, 869, 1324, 1692, 1760, 1785, 1934
HpaII CCGG 2 cut(s) 34, 106
HphI GGTGA 2 cut(s) 680, 2261
Hpy166II GTNNAC 6 cut(s) 752, 1730, 1813, 2115, 2382, 2599
Hpy188I TCNGA 9 cut(s) 688, 848, 1174, 1522, 1654, 1887, 2182, 2217, 2521
Hpy8I GTNNAC 6 cut(s) 752, 1730, 1813, 2115, 2382, 2599
Hpy99I CGWCG 3 cut(s) 23, 479, 1395
HpyAV CCTTC 5 cut(s) 53, 274, 1316, 2544, 2551
HpyCH4III ACNGT 7 cut(s) 565, 592, 1281, 1456, 1516, 1718, 2632
HpyCH4IV ACGT 2 cut(s) 574, 1015
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 491, 719, 943, 1898, 1918, 2054, 2068, 2282
HpyF3I CTNAG 8 cut(s) 341, 495, 618, 845, 1173, 1239, 1352, 2194
HpySE526I ACGT 2 cut(s) 574, 1015
HspAI GCGC 2 cut(s) 15, 2002
Ksp22I TGATCA 3 cut(s) 1264, 2104, 2319
LguI GCTCTTC 1 cut(s) 1159
LmnI GCTCC 7 cut(s) 28, 36, 345, 364, 934, 1376, 2068
LweI GCATC 6 cut(s) 280, 424, 837, 1448, 1527, 1885
MaeI CTAG 9 cut(s) 156, 242, 306, 519, 1197, 1428, 1778, 2091, 2234
MaeII ACGT 2 cut(s) 574, 1015
MaeIII GTNAC 6 cut(s) 565, 575, 586, 1220, 2140, 2632
MflI RGATCY 2 cut(s) 2167, 2400
MhlI GDGCHC 2 cut(s) 33, 1060
MlsI TGGCCA 2 cut(s) 149, 2258
MluNI TGGCCA 2 cut(s) 149, 2258
MlyI GAGTC 5 cut(s) 118, 222, 878, 1318, 1943
MmeI TCCRAC 2 cut(s) 876, 1475
Mox20I TGGCCA 2 cut(s) 149, 2258
Mph1103I ATGCAT 1 cut(s) 1857
MroXI GAANNNNTTC 3 cut(s) 1168, 1764, 2561
MscI TGGCCA 2 cut(s) 149, 2258
MseI TTAA 9 cut(s) 218, 293, 419, 824, 951, 1116, 1145, 1319, 2447
MslI CAYNNNNRTG 2 cut(s) 1042, 1850
Msp20I TGGCCA 2 cut(s) 149, 2258
MspA1I CMGCKG 3 cut(s) 193, 448, 1243
MspI CCGG 2 cut(s) 34, 106
MspR9I CCNGG 4 cut(s) 26, 107, 1626, 2081
Mva1269I GAATGC 1 cut(s) 1501
MvaI CCWGG 3 cut(s) 26, 1626, 2081
MvnI CGCG 2 cut(s) 141, 2062
MwoI GCNNNNNNNGC 9 cut(s) 47, 491, 719, 943, 1898, 1918, 2054, 2068, 2282
NciI CCSGG 1 cut(s) 107
NdeI CATATG 2 cut(s) 425, 1297
NlaIV GGNNCC 5 cut(s) 64, 347, 398, 549, 1089
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 4 cut(s) 565, 575, 1220, 2140
NsiI ATGCAT 1 cut(s) 1857
NspI RCATGY 8 cut(s) 115, 737, 965, 987, 1041, 1108, 1980, 2665
NspV TTCGAA 1 cut(s) 278
PaeI GCATGC 2 cut(s) 737, 1980
PagI TCATGA 1 cut(s) 1212
PceI AGGCCT 1 cut(s) 1261
PciI ACATGT 2 cut(s) 983, 2661
PciSI GCTCTTC 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 1399
PctI GAATGC 1 cut(s) 1501
PdmI GAANNNNTTC 3 cut(s) 1168, 1764, 2561
PfeI GAWTC 4 cut(s) 785, 1692, 1760, 1785
PleI GAGTC 5 cut(s) 118, 221, 877, 1318, 1942
PpsI GAGTC 5 cut(s) 118, 221, 877, 1318, 1942
Ppu21I YACGTR 1 cut(s) 1016
PpuMI RGGWCCY 1 cut(s) 548
PscI ACATGT 2 cut(s) 983, 2661
Psp124BI GAGCTC 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 3 cut(s) 24, 1624, 2079
PspFI CCCAGC 1 cut(s) 1921
PspGI CCWGG 3 cut(s) 24, 1624, 2079
PspN4I GGNNCC 5 cut(s) 64, 347, 398, 549, 1089
PspPI GGNCC 4 cut(s) 372, 548, 1087, 1346
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 2 cut(s) 559, 1504
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 2 cut(s) 2167, 2400
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 6 cut(s) 316, 1077, 1432, 1633, 2114, 2492
RsaNI GTAC 6 cut(s) 315, 1076, 1431, 1632, 2113, 2491
RseI CAYNNNNRTG 2 cut(s) 1042, 1850
SacI GAGCTC 1 cut(s) 33
SapI GCTCTTC 1 cut(s) 1159
SaqAI TTAA 9 cut(s) 218, 293, 419, 824, 951, 1116, 1145, 1319, 2447
Sau96I GGNCC 4 cut(s) 372, 548, 1087, 1346
SchI GAGTC 5 cut(s) 118, 222, 878, 1318, 1943
ScrFI CCNGG 4 cut(s) 26, 107, 1626, 2081
SduI GDGCHC 2 cut(s) 33, 1060
SfaNI GCATC 6 cut(s) 280, 424, 837, 1448, 1527, 1885
SfcI CTRYAG 2 cut(s) 555, 1500
SfuI TTCGAA 1 cut(s) 278
SinI GGWCC 3 cut(s) 372, 548, 1087
SmiMI CAYNNNNRTG 2 cut(s) 1042, 1850
SphI GCATGC 2 cut(s) 737, 1980
SseBI AGGCCT 1 cut(s) 1261
SsiI CCGC 8 cut(s) 51, 139, 193, 400, 883, 1126, 1243, 2437
SspI AATATT 2 cut(s) 224, 2177
SspMI CTAG 9 cut(s) 156, 242, 306, 519, 1197, 1428, 1778, 2091, 2234
SstI GAGCTC 1 cut(s) 33
StuI AGGCCT 1 cut(s) 1261
StyD4I CCNGG 4 cut(s) 24, 105, 1624, 2079
StyI CCWWGG 2 cut(s) 155, 1342
TaaI ACNGT 7 cut(s) 565, 592, 1281, 1456, 1516, 1718, 2632
TaiI ACGT 2 cut(s) 577, 1018
TaqI TCGA 7 cut(s) 57, 86, 211, 278, 477, 872, 930
TatI WGTACW 3 cut(s) 314, 1430, 2490
TauI GCSGC 2 cut(s) 53, 141
TfiI GAWTC 4 cut(s) 785, 1692, 1760, 1785
Tru1I TTAA 9 cut(s) 218, 293, 419, 824, 951, 1116, 1145, 1319, 2447
Tru9I TTAA 9 cut(s) 218, 293, 419, 824, 951, 1116, 1145, 1319, 2447
TscAI CASTG 5 cut(s) 235, 444, 570, 1356, 2488
TseFI GTSAC 4 cut(s) 565, 575, 1220, 2140
Tsp45I GTSAC 4 cut(s) 565, 575, 1220, 2140
TspDTI ATGAA 8 cut(s) 831, 990, 1201, 1508, 1773, 1986, 2304, 2592
TspGWI ACGGA 4 cut(s) 64, 80, 163, 986
TspRI CASTG 5 cut(s) 235, 444, 570, 1356, 2488
VpaK11BI GGWCC 3 cut(s) 372, 548, 1087
XapI RAATTY 3 cut(s) 1406, 2120, 2261
XbaI TCTAGA 1 cut(s) 241
XceI RCATGY 8 cut(s) 115, 737, 965, 987, 1041, 1108, 1980, 2665
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XmaJI CCTAGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 2381
XmnI GAANNNNTTC 3 cut(s) 1168, 1764, 2561
XspI CTAG 9 cut(s) 156, 242, 306, 519, 1197, 1428, 1778, 2091, 2234
Zsp2I ATGCAT 1 cut(s) 1857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.