Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0012804

Physical Location & Seq

Genomic Coordinates Forward (+)

2987564 .. 2989534

Gene Structure 1971 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1040400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGAGGACCGGCGGCGCCACCGTCTCCCTCCTCCTCTTCCTCTGCCTCCAGTTCCCACTTCAATTAATTCTCGCTTTCAATTCTTCCTCTCTAAACAACGACACGCGAATCTTGCAGGAAGTTCTGAAGCAAATCTCGGCCAAGCACAAGTGGGATTTGCAGGACGTGAGGGTCTCCAGGCTGGATGTGGGCAGGGTCCGATTCGGGAGCGCCCAGCGGTACGAGTTCCGGGTCGGATTCGGGAAGATCCACGTCGGCGTCTTGTTCTCTGACGACGTCGCTTCCTGGAAGAAGTTCAGGAAACCCAGGACCCATTTGGGCTCTCTCGTTCAGGAGCTCAGCTCCATGGCCGTTGTCGACACCTTCGAAGTGGAGGGTCCGTTTGAGTTGCGGGTCGCCGGCGCTCACGAGCTCTCGCTTTCATTGCCGATGAACACTACATATAATGGGTTGAAAAGGATTCTTGTTGGTGAAGGCATAACCGTGGAGGTAAGCAGAGCGATCGAAGTCTCTGTGTTTCATGCGTCTGATCTTGGTTTATCATCAAATGGCAGTGGTGCTACTAAGGAGAGGAGTGAGTTTTGGTCCATTGGGCATTCATACTGTGCCCCATTAGTTCCGATTCGTGTTTTTGGGCCGGCAACATTGGTTGCATACAAGACTCGAAATCATGATGCCCACATTGAAACTAATTTTATTTCCAAGGAGATAGTTGAATTGTTGCCAGAGAAGTGCTACAGAAGTCATGCATACAAGAAGCGGCCTTGCCCTGTTGATTCTTTAAGTTTGAGGATATCTATGCTGGAAAGAATTTGGAAAGGCTTTCTTGGTGATAGAATTCGCCAGAACAAATTGTCGGGATTTGTTGAAGGAAAAATCAAAGCTTCAACTATTGTTCGCTTCAAGGTTGAGCTCAAATTGGATTTTCGGAGGGTTGGTGCACTCCATGACAAAGATGGGTGGAGAACTAGGCCAGCTGTTCAACGGGTTTGGTTTGAAGTTTTGGCGAGAGTTGAATTAAAGAGGGTTAAGCCTCTCTTTGTTAGAAAGATTAGGCCCTTCATTGTAGCTGATTCAGTTGCATGGAGTAGTTTGACGTCAAATATTTCCTTTACCAAGTCCGGATCTGTCCTTGGCCCTCCTGAGGCGTTAACACTGGATGTGAAGTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0016020 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 map02024 map03060

Pfam Domains

Protein Families

Peptidase_S24 Peptidase_S26

Protein Analysis

391

Amino Acids

43.96

Weight (kDa)

9.95

Isoelectric Point (pI)

32.89

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012804)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47310
fragaria_vesca	FvH4_2g40681 FvH4_2g40681 FvH4_2g40681
malus_domestica	MD08G1040400.v1.1 MD15G1034900.v1.1
prunus_persica	Prupe.1G389000_v2.0.a1 Prupe.1G389000_v2.0.a1
pyrus_communis	pycom08g03260 pycom15g03210
rosa_chinensis	RchiOBHm_Chr6g0299921
rosa_multiflora	Rmu_co8499763.1_g000002
rosa_roxburghii	Rroxscaffold_7G00168270
rosa_rugosa	Rorug06G0293200
rosa_samantha	Rh6AG404600 Rh6BG412900 Rh6CG419000 Rh6DG405800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	170
AatII	GACGTC	2 cut(s)	279, 1100
AccB1I	GGYRCC	1 cut(s)	14
AccI	GTMKAC	1 cut(s)	357
AccII	CGCG	1 cut(s)	106
AccIII	TCCGGA	1 cut(s)	1121
AciI	CCGC	4 cut(s)	12, 217, 391, 760
AclWI	GGATC	2 cut(s)	241, 1132
AcoI	YGGCCR	2 cut(s)	138, 348
AcsI	RAATTY	2 cut(s)	810, 837
AcuI	CTGAAG	1 cut(s)	146
AcyI	GRCGYC	4 cut(s)	15, 258, 276, 1097
AfaI	GTAC	1 cut(s)	221
AfiI	CCNNNNNNNGG	1 cut(s)	1144
AjiI	CACGTC	2 cut(s)	166, 253
AjnI	CCWGG	3 cut(s)	176, 284, 305
AluBI	AGCT	7 cut(s)	337, 342, 412, 884, 913, 977, 1070
AluI	AGCT	7 cut(s)	337, 342, 412, 884, 913, 977, 1070
Alw21I	GWGCWC	4 cut(s)	339, 414, 915, 943
Alw26I	GTCTC	3 cut(s)	28, 178, 514
Alw44I	GTGCAC	1 cut(s)	939
AlwI	GGATC	2 cut(s)	241, 1132
Aor13HI	TCCGGA	1 cut(s)	1121
AoxI	GGCC	7 cut(s)	138, 348, 635, 761, 971, 1055, 1135
ApaLI	GTGCAC	1 cut(s)	939
ApoI	RAATTY	2 cut(s)	810, 837
AseI	ATTAAT	1 cut(s)	65
Asp700I	GAANNNNTTC	1 cut(s)	293
AspLEI	GCGC	3 cut(s)	17, 212, 404
AspS9I	GGNCC	8 cut(s)	6, 196, 309, 377, 585, 635, 1056, 1136
AsuC2I	CCSGG	1 cut(s)	230
AsuHPI	GGTGA	2 cut(s)	482, 842
AsuII	TTCGAA	1 cut(s)	366
AvaII	GGWCC	5 cut(s)	6, 196, 309, 377, 585
AxyI	CCTNAGG	1 cut(s)	1143
BaeGI	GKGCMC	2 cut(s)	610, 943
BanI	GGYRCC	1 cut(s)	14
BanII	GRGCYC	4 cut(s)	323, 339, 414, 915
BauI	CACGAG	1 cut(s)	407
Bbv12I	GWGCWC	4 cut(s)	339, 414, 915, 943
BccI	CCATC	1 cut(s)	950
BceAI	ACGGC	1 cut(s)	335
BcgI	CGANNNNNNTGC	2 cut(s)	484, 518
BciT130I	CCWGG	3 cut(s)	178, 286, 307
BcnI	CCSGG	1 cut(s)	230
BcoDI	GTCTC	3 cut(s)	28, 178, 514
BfaI	CTAG	1 cut(s)	969
BfmI	CTRYAG	1 cut(s)	736
BfoI	RGCGCY	3 cut(s)	18, 213, 405
BisI	GCNGC	2 cut(s)	13, 761
BlpI	GCTNAGC	1 cut(s)	338
BlsI	GCNGC	2 cut(s)	14, 762
Bme1390I	CCNGG	4 cut(s)	178, 230, 286, 307
Bme18I	GGWCC	5 cut(s)	6, 196, 309, 377, 585
BmgBI	CACGTC	2 cut(s)	166, 253
BmgT120I	GGNCC	8 cut(s)	6, 196, 309, 377, 585, 635, 1056, 1136
BmiI	GGNNCC	4 cut(s)	16, 197, 311, 378
BmrFI	CCNGG	4 cut(s)	178, 230, 286, 307
BmsI	GCATC	1 cut(s)	664
BplI	GAGNNNNNCTC	2 cut(s)	326, 358
BpmI	CTGGAG	2 cut(s)	32, 160
Bpu1102I	GCTNAGC	1 cut(s)	338
Bpu14I	TTCGAA	1 cut(s)	366
BpuMI	CCSGG	1 cut(s)	230
BsaHI	GRCGYC	4 cut(s)	15, 258, 276, 1097
BsaI	GGTCTC	1 cut(s)	178
BsaJI	CCNNGG	5 cut(s)	305, 345, 483, 702, 1132
BsaWI	WCCGGW	1 cut(s)	1121
BsaXI	ACNNNNNCTCC	4 cut(s)	365, 395, 922, 952
Bsc4I	CCNNNNNNNGG	1 cut(s)	1144
Bse118I	RCCGGY	3 cut(s)	8, 398, 637
Bse1I	ACTGG	2 cut(s)	49, 1161
Bse21I	CCTNAGG	1 cut(s)	1143
Bse3DI	GCAATG	1 cut(s)	422
BseAI	TCCGGA	1 cut(s)	1121
BseBI	CCWGG	3 cut(s)	178, 286, 307
BseDI	CCNNGG	5 cut(s)	305, 345, 483, 702, 1132
BseGI	GGATG	2 cut(s)	190, 1165
BseLI	CCNNNNNNNGG	1 cut(s)	1144
BseMI	GCAATG	1 cut(s)	422
BseMII	CTCAG	2 cut(s)	352, 1134
BseNI	ACTGG	2 cut(s)	49, 1161
BseRI	GAGGAG	3 cut(s)	20, 23, 586
BseSI	GKGCMC	2 cut(s)	610, 943
BseYI	CCCAGC	1 cut(s)	213
Bsh1236I	CGCG	1 cut(s)	106
Bsh1285I	CGRYCG	1 cut(s)	504
BshFI	GGCC	7 cut(s)	140, 350, 637, 763, 973, 1057, 1137
BshNI	GGYRCC	1 cut(s)	14
BsiEI	CGRYCG	1 cut(s)	504
BsiHKAI	GWGCWC	4 cut(s)	339, 414, 915, 943
BsiSI	CCGG	5 cut(s)	9, 229, 399, 638, 1122
BslI	CCNNNNNNNGG	1 cut(s)	1144
BsmAI	GTCTC	3 cut(s)	28, 178, 514
BsmBI	CGTCTC	1 cut(s)	28
BsmI	GAATGC	1 cut(s)	595
BsnI	GGCC	7 cut(s)	140, 350, 637, 763, 973, 1057, 1137
Bso31I	GGTCTC	1 cut(s)	178
Bsp119I	TTCGAA	1 cut(s)	366
Bsp1286I	GDGCHC	6 cut(s)	323, 339, 414, 610, 915, 943
Bsp13I	TCCGGA	1 cut(s)	1121
Bsp143I	GATC	4 cut(s)	246, 501, 529, 1124
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	1 cut(s)	345
BspACI	CCGC	4 cut(s)	12, 217, 391, 760
BspANI	GGCC	7 cut(s)	140, 350, 637, 763, 973, 1057, 1137
BspCNI	CTCAG	2 cut(s)	351, 1135
BspEI	TCCGGA	1 cut(s)	1121
BspFNI	CGCG	1 cut(s)	106
BspHI	TCATGA	1 cut(s)	670
BspLI	GGNNCC	4 cut(s)	16, 197, 311, 378
BspPI	GGATC	2 cut(s)	241, 1132
BspT104I	TTCGAA	1 cut(s)	366
BspT107I	GGYRCC	1 cut(s)	14
BspTNI	GGTCTC	1 cut(s)	178
BsrDI	GCAATG	1 cut(s)	422
BsrFI	RCCGGY	3 cut(s)	8, 398, 637
BsrI	ACTGG	2 cut(s)	49, 1161
BssAI	RCCGGY	3 cut(s)	8, 398, 637
BssECI	CCNNGG	5 cut(s)	305, 345, 483, 702, 1132
BssMI	GATC	4 cut(s)	246, 501, 529, 1124
BssNI	GRCGYC	4 cut(s)	15, 258, 276, 1097
BssSI	CACGAG	1 cut(s)	407
BssT1I	CCWWGG	3 cut(s)	345, 702, 1132
Bst2BI	CACGAG	1 cut(s)	407
Bst2UI	CCWGG	3 cut(s)	178, 286, 307
Bst4CI	ACNGT	3 cut(s)	22, 484, 605
Bst6I	CTCTTC	1 cut(s)	41
BstACI	GRCGYC	4 cut(s)	15, 258, 276, 1097
BstBI	TTCGAA	1 cut(s)	366
BstC8I	GCNNGC	3 cut(s)	400, 639, 975
BstDEI	CTNAG	3 cut(s)	338, 564, 1143
BstDSI	CCRYGG	2 cut(s)	345, 483
BstF5I	GGATG	2 cut(s)	190, 1165
BstFNI	CGCG	1 cut(s)	106
BstH2I	RGCGCY	3 cut(s)	18, 213, 405
BstHHI	GCGC	3 cut(s)	17, 212, 404
BstKTI	GATC	4 cut(s)	249, 504, 532, 1127
BstMAI	GTCTC	3 cut(s)	28, 178, 514
BstMBI	GATC	4 cut(s)	246, 501, 529, 1124
BstMCI	CGRYCG	1 cut(s)	504
BstMWI	GCNNNNNNNGC	2 cut(s)	112, 424
BstNI	CCWGG	3 cut(s)	178, 286, 307
BstSCI	CCNGG	4 cut(s)	176, 228, 284, 305
BstSFI	CTRYAG	1 cut(s)	736
BstSLI	GKGCMC	2 cut(s)	610, 943
BstUI	CGCG	1 cut(s)	106
BstX2I	RGATCY	2 cut(s)	246, 1124
BstYI	RGATCY	2 cut(s)	246, 1124
Bsu36I	CCTNAGG	1 cut(s)	1143
BsuRI	GGCC	7 cut(s)	140, 350, 637, 763, 973, 1057, 1137
BtgI	CCRYGG	2 cut(s)	345, 483
BtrI	CACGTC	2 cut(s)	166, 253
BtsCI	GGATG	2 cut(s)	190, 1165
BtsI	GCAGTG	1 cut(s)	559
BtsIMutI	CAGTG	2 cut(s)	559, 1154
Cac8I	GCNNGC	3 cut(s)	400, 639, 975
CciI	TCATGA	1 cut(s)	670
CfoI	GCGC	3 cut(s)	17, 212, 404
Cfr10I	RCCGGY	3 cut(s)	8, 398, 637
Cfr13I	GGNCC	8 cut(s)	6, 196, 309, 377, 585, 635, 1056, 1136
CseI	GACGC	2 cut(s)	247, 513
Csp6I	GTAC	1 cut(s)	220
CviAII	CATG	6 cut(s)	346, 521, 671, 746, 947, 1083
CviQI	GTAC	1 cut(s)	220
DdeI	CTNAG	3 cut(s)	338, 564, 1143
DinI	GGCGCC	1 cut(s)	16
DpnI	GATC	4 cut(s)	248, 503, 531, 1126
DpnII	GATC	4 cut(s)	246, 501, 529, 1124
DrdI	GACNNNNNNGTC	1 cut(s)	170
DseDI	GACNNNNNNGTC	1 cut(s)	170
EaeI	YGGCCR	2 cut(s)	138, 348
Eam1104I	CTCTTC	1 cut(s)	41
EarI	CTCTTC	1 cut(s)	41
Ecl136II	GAGCTC	3 cut(s)	337, 412, 913
Eco130I	CCWWGG	3 cut(s)	345, 702, 1132
Eco24I	GRGCYC	4 cut(s)	323, 339, 414, 915
Eco31I	GGTCTC	1 cut(s)	178
Eco32I	GATATC	1 cut(s)	795
Eco47I	GGWCC	5 cut(s)	6, 196, 309, 377, 585
Eco53kI	GAGCTC	3 cut(s)	337, 412, 913
Eco57I	CTGAAG	1 cut(s)	146
Eco81I	CCTNAGG	1 cut(s)	1143
EcoICRI	GAGCTC	3 cut(s)	337, 412, 913
EcoO109I	RGGNCCY	2 cut(s)	309, 1056
EcoRI	GAATTC	1 cut(s)	837
EcoRII	CCWGG	3 cut(s)	176, 284, 305
EcoRV	GATATC	1 cut(s)	795
EcoT14I	CCWWGG	3 cut(s)	345, 702, 1132
EcoT22I	ATGCAT	1 cut(s)	751
EcoT38I	GRGCYC	4 cut(s)	323, 339, 414, 915
EgeI	GGCGCC	1 cut(s)	16
EheI	GGCGCC	1 cut(s)	16
ErhI	CCWWGG	3 cut(s)	345, 702, 1132
Esp3I	CGTCTC	1 cut(s)	28
FaeI	CATG	6 cut(s)	349, 524, 674, 749, 950, 1086
FalI	AAGNNNNNCTT	4 cut(s)	810, 842, 1022, 1054
FatI	CATG	6 cut(s)	345, 520, 670, 745, 946, 1082
FauI	CCCGC	1 cut(s)	384
FblI	GTMKAC	1 cut(s)	357
Fnu4HI	GCNGC	2 cut(s)	13, 761
FokI	GGATG	1 cut(s)	197
FriOI	GRGCYC	4 cut(s)	323, 339, 414, 915
Fsp4HI	GCNGC	2 cut(s)	13, 761
FspBI	CTAG	1 cut(s)	969
GlaI	GCGC	3 cut(s)	16, 211, 403
GluI	GCNGC	2 cut(s)	13, 761
GsaI	CCCAGC	1 cut(s)	217
GsuI	CTGGAG	2 cut(s)	32, 160
HaeII	RGCGCY	3 cut(s)	18, 213, 405
HaeIII	GGCC	7 cut(s)	140, 350, 637, 763, 973, 1057, 1137
HapII	CCGG	5 cut(s)	9, 229, 399, 638, 1122
HgaI	GACGC	2 cut(s)	247, 513
HhaI	GCGC	3 cut(s)	17, 212, 404
Hin1I	GRCGYC	4 cut(s)	15, 258, 276, 1097
Hin1II	CATG	6 cut(s)	349, 524, 674, 749, 950, 1086
Hin6I	GCGC	3 cut(s)	15, 210, 402
HinP1I	GCGC	3 cut(s)	15, 210, 402
HincII	GTYRAC	2 cut(s)	358, 1152
HindII	GTYRAC	2 cut(s)	358, 1152
HindIII	AAGCTT	1 cut(s)	882
HinfI	GANTC	8 cut(s)	108, 201, 237, 460, 622, 661, 776, 1073
HpaI	GTTAAC	1 cut(s)	1152
HpaII	CCGG	5 cut(s)	9, 229, 399, 638, 1122
HphI	GGTGA	2 cut(s)	482, 842
Hpy166II	GTNNAC	3 cut(s)	358, 941, 1152
Hpy188I	TCNGA	7 cut(s)	126, 200, 236, 271, 529, 621, 930
Hpy188III	TCNNGA	9 cut(s)	205, 241, 298, 332, 407, 671, 858, 1122, 1142
Hpy8I	GTNNAC	3 cut(s)	358, 941, 1152
Hpy99I	CGWCG	3 cut(s)	257, 278, 281
HpyAV	CCTTC	4 cut(s)	373, 467, 863, 1069
HpyCH4III	ACNGT	3 cut(s)	22, 484, 605
HpyCH4IV	ACGT	4 cut(s)	165, 252, 276, 1097
HpyCH4V	TGCA	6 cut(s)	115, 160, 653, 749, 941, 1082
HpyF10VI	GCNNNNNNNGC	2 cut(s)	112, 424
HpyF3I	CTNAG	3 cut(s)	338, 564, 1143
HpySE526I	ACGT	4 cut(s)	165, 252, 276, 1097
Hsp92I	GRCGYC	4 cut(s)	15, 258, 276, 1097
Hsp92II	CATG	6 cut(s)	349, 524, 674, 749, 950, 1086
HspAI	GCGC	3 cut(s)	15, 210, 402
KasI	GGCGCC	1 cut(s)	14
Kpn2I	TCCGGA	1 cut(s)	1121
KroI	GCCGGC	2 cut(s)	398, 637
KroNI	GCCGGC	2 cut(s)	400, 639
KspAI	GTTAAC	1 cut(s)	1152
Kzo9I	GATC	4 cut(s)	246, 501, 529, 1124
LmnI	GCTCC	3 cut(s)	207, 334, 347
LweI	GCATC	1 cut(s)	664
MaeI	CTAG	1 cut(s)	969
MaeII	ACGT	4 cut(s)	165, 252, 276, 1097
MalI	GATC	4 cut(s)	248, 503, 531, 1126
MboI	GATC	4 cut(s)	246, 501, 529, 1124
MboII	GAAGA	4 cut(s)	28, 75, 256, 301
MflI	RGATCY	2 cut(s)	246, 1124
MhlI	GDGCHC	6 cut(s)	323, 339, 414, 610, 915, 943
Mly113I	GGCGCC	1 cut(s)	15
MlyI	GAGTC	1 cut(s)	655
MmeI	TCCRAC	1 cut(s)	214
Mph1103I	ATGCAT	1 cut(s)	751
MreI	CGCCGGCG	1 cut(s)	398
MroI	TCCGGA	1 cut(s)	1121
MroNI	GCCGGC	2 cut(s)	398, 637
MroXI	GAANNNNTTC	1 cut(s)	293
MseI	TTAA	5 cut(s)	65, 782, 1019, 1029, 1151
MslI	CAYNNNNRTG	1 cut(s)	482
MspA1I	CMGCKG	2 cut(s)	217, 977
MspI	CCGG	5 cut(s)	9, 229, 399, 638, 1122
MspR9I	CCNGG	4 cut(s)	178, 230, 286, 307
Mva1269I	GAATGC	1 cut(s)	595
MvaI	CCWGG	3 cut(s)	178, 286, 307
MvnI	CGCG	1 cut(s)	106
MwoI	GCNNNNNNNGC	2 cut(s)	112, 424
NaeI	GCCGGC	2 cut(s)	400, 639
NarI	GGCGCC	1 cut(s)	15
NciI	CCSGG	1 cut(s)	230
NcoI	CCATGG	1 cut(s)	345
NdeII	GATC	4 cut(s)	246, 501, 529, 1124
NgoMIV	GCCGGC	2 cut(s)	398, 637
NlaIII	CATG	6 cut(s)	349, 524, 674, 749, 950, 1086
NlaIV	GGNNCC	4 cut(s)	16, 197, 311, 378
NmeAIII	GCCGAG	1 cut(s)	116
NsiI	ATGCAT	1 cut(s)	751
NspV	TTCGAA	1 cut(s)	366
PagI	TCATGA	1 cut(s)	670
PcsI	WCGNNNNNNNCGW	1 cut(s)	363
PctI	GAATGC	1 cut(s)	595
PdiI	GCCGGC	2 cut(s)	400, 639
PdmI	GAANNNNTTC	1 cut(s)	293
PfeI	GAWTC	7 cut(s)	108, 201, 237, 460, 622, 776, 1073
PflFI	GACNNNGTC	1 cut(s)	275
PfoI	TCCNGGA	1 cut(s)	284
PkrI	GCNGC	2 cut(s)	14, 762
Ple19I	CGATCG	1 cut(s)	504
PleI	GAGTC	1 cut(s)	655
PluTI	GGCGCC	1 cut(s)	18
PpsI	GAGTC	1 cut(s)	655
PpuMI	RGGWCCY	1 cut(s)	309
PshBI	ATTAAT	1 cut(s)	65
Psp124BI	GAGCTC	3 cut(s)	339, 414, 915
Psp5II	RGGWCCY	1 cut(s)	309
Psp6I	CCWGG	3 cut(s)	176, 284, 305
PspFI	CCCAGC	1 cut(s)	213
PspGI	CCWGG	3 cut(s)	176, 284, 305
PspN4I	GGNNCC	4 cut(s)	16, 197, 311, 378
PspPI	GGNCC	8 cut(s)	6, 196, 309, 377, 585, 635, 1056, 1136
PspPPI	RGGWCCY	1 cut(s)	309
PsuI	RGATCY	2 cut(s)	246, 1124
PsyI	GACNNNGTC	1 cut(s)	275
PvuI	CGATCG	1 cut(s)	504
PvuII	CAGCTG	1 cut(s)	977
RsaI	GTAC	1 cut(s)	221
RsaNI	GTAC	1 cut(s)	220
RseI	CAYNNNNRTG	1 cut(s)	482
SacI	GAGCTC	3 cut(s)	339, 414, 915
SalI	GTCGAC	1 cut(s)	356
SaqAI	TTAA	5 cut(s)	65, 782, 1019, 1029, 1151
SatI	GCNGC	2 cut(s)	13, 761
Sau3AI	GATC	4 cut(s)	246, 501, 529, 1124
Sau96I	GGNCC	8 cut(s)	6, 196, 309, 377, 585, 635, 1056, 1136
SchI	GAGTC	1 cut(s)	655
ScrFI	CCNGG	4 cut(s)	178, 230, 286, 307
SduI	GDGCHC	6 cut(s)	323, 339, 414, 610, 915, 943
SfaNI	GCATC	1 cut(s)	664
SfcI	CTRYAG	1 cut(s)	736
SfoI	GGCGCC	1 cut(s)	16
SfuI	TTCGAA	1 cut(s)	366
SgrAI	CRCCGGYG	1 cut(s)	398
SinI	GGWCC	5 cut(s)	6, 196, 309, 377, 585
SmiMI	CAYNNNNRTG	1 cut(s)	482
SsiI	CCGC	4 cut(s)	12, 217, 391, 760
SspDI	GGCGCC	1 cut(s)	14
SspI	AATATT	1 cut(s)	1105
SspMI	CTAG	1 cut(s)	969
SstI	GAGCTC	3 cut(s)	339, 414, 915
StyD4I	CCNGG	4 cut(s)	176, 228, 284, 305
StyI	CCWWGG	3 cut(s)	345, 702, 1132
TaaI	ACNGT	3 cut(s)	22, 484, 605
TaiI	ACGT	4 cut(s)	168, 255, 279, 1100
TaqI	TCGA	4 cut(s)	357, 366, 504, 664
TauI	GCSGC	2 cut(s)	15, 763
TfiI	GAWTC	7 cut(s)	108, 201, 237, 460, 622, 776, 1073
Tru1I	TTAA	5 cut(s)	65, 782, 1019, 1029, 1151
Tru9I	TTAA	5 cut(s)	65, 782, 1019, 1029, 1151
TscAI	CASTG	2 cut(s)	559, 1161
TspDTI	ATGAA	5 cut(s)	411, 446, 509, 588, 1051
TspGWI	ACGGA	1 cut(s)	369
TspRI	CASTG	2 cut(s)	559, 1161
Tth111I	GACNNNGTC	1 cut(s)	275
VneI	GTGCAC	1 cut(s)	939
VpaK11BI	GGWCC	5 cut(s)	6, 196, 309, 377, 585
VspI	ATTAAT	1 cut(s)	65
XapI	RAATTY	2 cut(s)	810, 837
XcmI	CCANNNNNNNNNTGG	3 cut(s)	184, 313, 953
XmiI	GTMKAC	1 cut(s)	357
XmnI	GAANNNNTTC	1 cut(s)	293
XspI	CTAG	1 cut(s)	969
ZraI	GACGTC	2 cut(s)	277, 1098
Zsp2I	ATGCAT	1 cut(s)	751

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.