MD08G1052900.v1.1

Ubiquitinyl hydrolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012946
Physical Location & Seq
Forward (+)
4136747 .. 4139658
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1052900.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGTCGACAAGGAAGCCATCATACGCAAACCCAAAACCCTGCAACCACCTCTCAGCTTACAAGCTCAAACATGGCTCCAGCGGCTACAAATCCCTCCAAAAATCCCTCAAATCTGCCCCCAACGGAAGAACAAGCGTTCGGATTCAGGAAACTAAGGTACCCAGATGCAGTGTCTGTAATGGGTTCGAAGGTAGACTCTATATTTGCTTGATTTGCTCTTCGGTTTCGTGTTCGAATCACGCAGTTCTGCATACCCAGTTGGAAATCGGCCACGATTTGGCCGTCGATGTCCAAAGGGCCGAGCTTTTTTGCTGTGCGTGCTCCGATCAGGTCTACGATCCTGATTTCGATAAGTGCGTGATGGCTAAGCACATGCTGGACTTGCAGTGCAGCACAAATAGTGCGGATAGTATTGGGGAGAGACTGTGTGAGAGGAAGAGGCTGGGGGGTTCGGGTGTCGAATTATCGGAGTTTAAAAAGCCCGAATTATCGGGTTTGGTGAGGGATCACAGGGCGAAATCGTGTTATCCGTTGGGTTTGAGGGGGTTGAATAATTTGGGGAGTACTTGTTTTATGAACTCTGTGTTGCAAGCGCTGCTACATGCGCCTCCATTGAGGAATTACTTCTTGAGTGATCGGCATAGCCGCAGAACATGCCGGAAAAGGACGGCAAACCGGCTGTGTTTGCCTTGTGAGCTTCACGGTGTTTATTCGGCTGTGTATTCCGGCGATTGGACTCCTTATAGCCCGGCTCAATTGCTTTACAGTTGGTGGCAGCATTCGGAGAATCTGGCAAGCTACGAGCAGCAGGACGCTCATGAGTTCTTCATTTCAGTGTTGGATGGGATCCATGAGAAAGAAACCAAAGCAAGGAACCATACTAAAGATAGTGGAGACTGCCAGTGTATTGCGCATAGGGTATTCTCAGGACTGTTGAGGTCGGATGTCACTTGTATGACTTGTGGATTCACCTCGACAACTTATGACCCGTGTTTGGACATATCACTCAACTTAGACACAAGCCGCTGTTCTTGGTCAGATGCATCTAACAAGCCTGCCAAAGCGAATGGCAGCAGCTGTTCATCCACTCTTTTGGGTTGTTTAGACTTGTTTACAAAACCAGAAAAGTTGGGGTCAGACCAGAAACTGTACTGTCAGAACTGTCAAGAACTCCGAGACTCTTCAAAGCAAATGTCCATCAGAAAGCTTCCAATGGTGTTGTGTTTGCATATAAAACGGTTTGAGCATTCCCTGGTCAGAAAGATGATGAGGAAAATCGATAGCTATTTGCATTTTCCCTTCTCGCTAGACATGACTCCCTATCTGTCTTCTTCGATCGTCAGAAACAGATTTGGAAATAGAATCTTTGCTTTCGAGGGGGATGAATCTGAGATGTCTACCGAGTTTGAGATTTTTGCAGTAGTTACTCATTCCGGGACACTAGATTCCGGCCATTATGTGACGTATTTACGCATACAAGACCAGTGGTACAAATGTGACAATGCTTGGATTAATGAGGTCGACGAGGGGATTGTAAGAGCTTCACAGTGTTATATGATGTTCTACATGCACAAGACGCTGTACCATAAAGCAAACGTAGATTTGCGTCACTTGCCGGTTTCCACACAGAAGGAGGCATTTCAGAGGATGGCTGGTTGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

555

Amino Acids

62.78

Weight (kDa)

8.49

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 56 - 115 1.4e-10 Zn-finger in ubiquitin-hydrolases and other protein
UCH PF00443 181 - 522 2.3e-57 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 182 - 497 4.6e-18 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10790 AT5G10790
fragaria_vesca FvH4_2g41240
malus_domestica MD08G1052900.v1.1 MD15G1042700.v1.1
prunus_persica Prupe.1G396000_v2.0.a1
pyrus_communis pycom08g04410 pycom15g03980
rosa_chinensis RchiOBHm_Chr6g0299261
rosa_laevigata RLG00000011448
rosa_multiflora Rmu_sc0001434.1_g000005
rosa_roxburghii Rroxscaffold_7G00168850
rosa_rugosa Rorug06G0286700
rosa_samantha Rh6AG398000 Rh6BG406500 Rh6CG412600 Rh6DG399300
rosa_wichuraiana Rw6G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 912
Acc65I GGTACC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 157
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
AciI CCGC 4 cut(s) 81, 404, 646, 1024
AclWI GGATC 4 cut(s) 332, 513, 841, 854
AcoI YGGCCR 3 cut(s) 268, 279, 1450
AfaI GTAC 5 cut(s) 159, 565, 1151, 1490, 1583
AfeI AGCGCT 1 cut(s) 594
AfiI CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
AgsI TTSAA 2 cut(s) 550, 1185
AjnI CCWGG 1 cut(s) 1251
AluBI AGCT 9 cut(s) 56, 64, 304, 697, 798, 1077, 1207, 1284, 1541
AluI AGCT 9 cut(s) 56, 64, 304, 697, 798, 1077, 1207, 1284, 1541
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 3 cut(s) 415, 888, 1170
AlwI GGATC 4 cut(s) 332, 513, 841, 854
AlwNI CAGNNNCTG 3 cut(s) 174, 1077, 1147
Aor51HI AGCGCT 1 cut(s) 594
AoxI GGCC 4 cut(s) 268, 279, 297, 1450
ApeKI GCWGC 7 cut(s) 390, 595, 775, 805, 1071, 1074, 1659
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 1178, 1210
AseI ATTAAT 1 cut(s) 1512
Asp700I GAANNNNTTC 1 cut(s) 623
Asp718I GGTACC 1 cut(s) 157
AspLEI GCGC 3 cut(s) 595, 607, 913
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 2 cut(s) 749, 1435
AsuHPI GGTGA 2 cut(s) 511, 961
AsuII TTCGAA 2 cut(s) 186, 233
BaeI ACNNNNGTAYC 4 cut(s) 141, 174, 1480, 1513
BamHI GGATCC 1 cut(s) 846
BanI GGYRCC 1 cut(s) 157
BarI GAAGNNNNNNTAC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 1320
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 7 cut(s) 402, 582, 787, 817, 1083, 1086, 1646
BccI CCATC 5 cut(s) 25, 355, 836, 1205, 1642
BceAI ACGGC 2 cut(s) 266, 684
BciT130I CCWGG 1 cut(s) 1253
BcnI CCSGG 2 cut(s) 749, 1435
BcoDI GTCTC 3 cut(s) 415, 888, 1170
BfaI CTAG 3 cut(s) 1307, 1442, 1663
BfoI RGCGCY 1 cut(s) 596
BlpI GCTNAGC 1 cut(s) 366
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 3 cut(s) 749, 1253, 1435
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 4 cut(s) 76, 159, 848, 875
BmrFI CCNGG 3 cut(s) 749, 1253, 1435
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 3 cut(s) 155, 1030, 1052
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 1320
BpmI CTGGAG 1 cut(s) 61
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 2 cut(s) 186, 233
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 2 cut(s) 749, 1435
Bsa29I ATCGAT 1 cut(s) 1278
BsaJI CCNNGG 1 cut(s) 1251
BsaXI ACNNNNNCTCC 4 cut(s) 410, 440, 553, 583
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
Bse118I RCCGGY 2 cut(s) 675, 1615
Bse1I ACTGG 3 cut(s) 256, 901, 1483
BseBI CCWGG 1 cut(s) 1253
BseCI ATCGAT 1 cut(s) 1278
BseDI CCNNGG 1 cut(s) 1251
BseGI GGATG 5 cut(s) 847, 949, 1082, 1387, 1653
BseLI CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
BseMII CTCAG 3 cut(s) 66, 939, 1380
BseNI ACTGG 3 cut(s) 256, 901, 1483
BseXI GCAGC 7 cut(s) 402, 582, 787, 817, 1083, 1086, 1646
BseYI CCCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 409
Bsh1285I CGRYCG 1 cut(s) 1338
BshFI GGCC 4 cut(s) 270, 281, 299, 1452
BshNI GGYRCC 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 1278
BsiEI CGRYCG 1 cut(s) 1338
BsiHKAI GWGCWC 1 cut(s) 323
BsiSI CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
BslFI GGGAC 1 cut(s) 1450
BslI CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
BsmAI GTCTC 3 cut(s) 415, 888, 1170
BsmFI GGGAC 1 cut(s) 1450
BsmI GAATGC 2 cut(s) 778, 1246
BsnI GGCC 4 cut(s) 270, 281, 299, 1452
Bsp119I TTCGAA 2 cut(s) 186, 233
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 4 cut(s) 81, 404, 646, 1024
BspANI GGCC 4 cut(s) 270, 281, 299, 1452
BspCNI CTCAG 3 cut(s) 65, 938, 1381
BspDI ATCGAT 1 cut(s) 1278
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 4 cut(s) 76, 159, 848, 875
BspPI GGATC 4 cut(s) 332, 513, 841, 854
BspQI GCTCTTC 1 cut(s) 223
BspT104I TTCGAA 2 cut(s) 186, 233
BspT107I GGYRCC 1 cut(s) 157
BsrFI RCCGGY 2 cut(s) 675, 1615
BsrI ACTGG 3 cut(s) 256, 901, 1483
BssAI RCCGGY 2 cut(s) 675, 1615
BssECI CCNNGG 1 cut(s) 1251
BssMI GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
Bst2UI CCWGG 1 cut(s) 1253
Bst4CI ACNGT 9 cut(s) 426, 704, 767, 933, 1149, 1154, 1163, 1239, 1548
Bst6I CTCTTC 3 cut(s) 223, 431, 1186
BstAPI GCANNNNNTGC 2 cut(s) 595, 654
BstBI TTCGAA 2 cut(s) 186, 233
BstC8I GCNNGC 4 cut(s) 319, 591, 796, 1056
BstDEI CTNAG 6 cut(s) 52, 153, 366, 925, 1012, 1389
BstF5I GGATG 5 cut(s) 847, 949, 1082, 1387, 1653
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 3 cut(s) 595, 607, 913
BstKTI GATC 6 cut(s) 328, 340, 508, 637, 849, 1338
BstMAI GTCTC 3 cut(s) 415, 888, 1170
BstMBI GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
BstMCI CGRYCG 1 cut(s) 1338
BstNI CCWGG 1 cut(s) 1253
BstNSI RCATGY 4 cut(s) 376, 605, 657, 1570
BstSCI CCNGG 3 cut(s) 747, 1251, 1433
BstV1I GCAGC 7 cut(s) 402, 582, 787, 817, 1083, 1086, 1646
BstV2I GAAGAC 1 cut(s) 1320
BstX2I RGATCY 1 cut(s) 846
BstXI CCANNNNNNTGG 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 846
Bsu15I ATCGAT 1 cut(s) 1278
BsuRI GGCC 4 cut(s) 270, 281, 299, 1452
BsuTUI ATCGAT 1 cut(s) 1278
BtsCI GGATG 5 cut(s) 847, 949, 1082, 1387, 1653
BtsI GCAGTG 2 cut(s) 175, 392
BtsIMutI CAGTG 6 cut(s) 175, 392, 840, 908, 1490, 1553
Cac8I GCNNGC 4 cut(s) 319, 591, 796, 1056
CaiI CAGNNNCTG 3 cut(s) 174, 1077, 1147
CciI TCATGA 1 cut(s) 817
CfoI GCGC 3 cut(s) 595, 607, 913
Cfr10I RCCGGY 2 cut(s) 675, 1615
Cfr13I GGNCC 1 cut(s) 297
ClaI ATCGAT 1 cut(s) 1278
CseI GACGC 3 cut(s) 821, 1585, 1595
Csp6I GTAC 5 cut(s) 158, 564, 1150, 1489, 1582
CspCI CAANNNNNGTGG 2 cut(s) 1466, 1501
CviAII CATG 8 cut(s) 71, 373, 602, 654, 818, 851, 1312, 1567
CviQI GTAC 5 cut(s) 158, 564, 1150, 1489, 1582
DdeI CTNAG 6 cut(s) 52, 153, 366, 925, 1012, 1389
DpnI GATC 6 cut(s) 327, 339, 507, 636, 848, 1337
DpnII GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 3 cut(s) 268, 279, 1450
Eam1104I CTCTTC 3 cut(s) 223, 431, 1186
EarI CTCTTC 3 cut(s) 223, 431, 1186
Eco47III AGCGCT 1 cut(s) 594
EcoRII CCWGG 1 cut(s) 1251
EcoT22I ATGCAT 1 cut(s) 1045
FaeI CATG 8 cut(s) 74, 376, 605, 657, 821, 854, 1315, 1570
FaqI GGGAC 1 cut(s) 1450
FatI CATG 8 cut(s) 70, 372, 601, 653, 817, 850, 1311, 1566
FblI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
FokI GGATG 5 cut(s) 854, 956, 1069, 1394, 1660
FspBI CTAG 3 cut(s) 1307, 1442, 1663
FspI TGCGCA 1 cut(s) 912
GlaI GCGC 3 cut(s) 594, 606, 912
GsaI CCCAGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 61
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 4 cut(s) 270, 281, 299, 1452
HapII CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
HgaI GACGC 3 cut(s) 821, 1585, 1595
HhaI GCGC 3 cut(s) 595, 607, 913
Hin1II CATG 8 cut(s) 74, 376, 605, 657, 821, 854, 1315, 1570
Hin6I GCGC 3 cut(s) 593, 605, 911
HinP1I GCGC 3 cut(s) 593, 605, 911
HincII GTYRAC 2 cut(s) 6, 1522
HindII GTYRAC 2 cut(s) 6, 1522
HindIII AAGCTT 1 cut(s) 1205
HpaII CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
HphI GGTGA 2 cut(s) 511, 961
Hpy166II GTNNAC 6 cut(s) 6, 194, 334, 1113, 1398, 1522
Hpy188III TCNNGA 6 cut(s) 146, 341, 628, 818, 927, 1166
Hpy8I GTNNAC 6 cut(s) 6, 194, 334, 1113, 1398, 1522
Hpy99I CGWCG 2 cut(s) 287, 1526
HpyAV CCTTC 3 cut(s) 182, 1309, 1624
HpyCH4III ACNGT 9 cut(s) 426, 704, 767, 933, 1149, 1154, 1163, 1239, 1548
HpyCH4IV ACGT 2 cut(s) 1463, 1596
HpyF3I CTNAG 6 cut(s) 52, 153, 366, 925, 1012, 1389
HpySE526I ACGT 2 cut(s) 1463, 1596
Hsp92II CATG 8 cut(s) 74, 376, 605, 657, 821, 854, 1315, 1570
HspAI GCGC 3 cut(s) 593, 605, 911
KpnI GGTACC 1 cut(s) 161
Kzo9I GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 80, 326
Lsp1109I GCAGC 7 cut(s) 402, 582, 787, 817, 1083, 1086, 1646
LweI GCATC 3 cut(s) 155, 1030, 1052
MaeI CTAG 3 cut(s) 1307, 1442, 1663
MaeII ACGT 2 cut(s) 1463, 1596
MaeIII GTNAC 5 cut(s) 946, 1423, 1459, 1496, 1607
MalI GATC 6 cut(s) 327, 339, 507, 636, 848, 1337
MboI GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
MboII GAAGA 7 cut(s) 138, 210, 448, 817, 1173, 1320, 1323
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 1 cut(s) 846
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 5 cut(s) 461, 485, 553, 619, 755
MlyI GAGTC 4 cut(s) 189, 730, 1172, 1309
MmeI TCCRAC 3 cut(s) 240, 819, 921
Mph1103I ATGCAT 1 cut(s) 1045
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 2 cut(s) 474, 1512
MslI CAYNNNNRTG 2 cut(s) 833, 953
MspA1I CMGCKG 3 cut(s) 81, 1026, 1077
MspI CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
MspR9I CCNGG 3 cut(s) 749, 1253, 1435
MunI CAATTG 1 cut(s) 755
Mva1269I GAATGC 2 cut(s) 778, 1246
MvaI CCWGG 1 cut(s) 1253
NciI CCSGG 2 cut(s) 749, 1435
NdeII GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
NlaIII CATG 8 cut(s) 74, 376, 605, 657, 821, 854, 1315, 1570
NlaIV GGNNCC 4 cut(s) 76, 159, 848, 875
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 4 cut(s) 946, 1459, 1496, 1607
NsbI TGCGCA 1 cut(s) 912
NsiI ATGCAT 1 cut(s) 1045
NspI RCATGY 4 cut(s) 376, 605, 657, 1570
NspV TTCGAA 2 cut(s) 186, 233
PagI TCATGA 1 cut(s) 817
PciSI GCTCTTC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 354, 527
PctI GAATGC 2 cut(s) 778, 1246
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 7 cut(s) 142, 235, 787, 966, 1362, 1385, 1445
PflMI CCANNNNNTGG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 1433
Ple19I CGATCG 1 cut(s) 1338
PleI GAGTC 4 cut(s) 189, 730, 1172, 1309
PpsI GAGTC 4 cut(s) 189, 730, 1172, 1309
PshBI ATTAAT 1 cut(s) 1512
Psp6I CCWGG 1 cut(s) 1251
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 1251
PspN4I GGNNCC 4 cut(s) 76, 159, 848, 875
PspPI GGNCC 1 cut(s) 297
PstNI CAGNNNCTG 3 cut(s) 174, 1077, 1147
PsuI RGATCY 1 cut(s) 846
PvuI CGATCG 1 cut(s) 1338
PvuII CAGCTG 1 cut(s) 1077
RsaI GTAC 5 cut(s) 159, 565, 1151, 1490, 1583
RsaNI GTAC 5 cut(s) 158, 564, 1150, 1489, 1582
RseI CAYNNNNRTG 2 cut(s) 833, 953
SalI GTCGAC 2 cut(s) 4, 1520
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 2 cut(s) 474, 1512
Sau3AI GATC 6 cut(s) 325, 337, 505, 634, 846, 1335
Sau96I GGNCC 1 cut(s) 297
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 4 cut(s) 189, 730, 1172, 1309
ScrFI CCNGG 3 cut(s) 749, 1253, 1435
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 3 cut(s) 155, 1030, 1052
SfuI TTCGAA 2 cut(s) 186, 233
SmiMI CAYNNNNRTG 2 cut(s) 833, 953
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
Sse9I AATT 5 cut(s) 461, 485, 553, 619, 755
SsiI CCGC 4 cut(s) 81, 404, 646, 1024
SspMI CTAG 3 cut(s) 1307, 1442, 1663
StyD4I CCNGG 3 cut(s) 747, 1251, 1433
TaaI ACNGT 9 cut(s) 426, 704, 767, 933, 1149, 1154, 1163, 1239, 1548
TaiI ACGT 2 cut(s) 1466, 1599
TasI AATT 5 cut(s) 461, 485, 553, 619, 755
TatI WGTACW 2 cut(s) 563, 1149
TauI GCSGC 3 cut(s) 84, 648, 1026
TfiI GAWTC 7 cut(s) 142, 235, 787, 966, 1362, 1385, 1445
Tru1I TTAA 2 cut(s) 474, 1512
Tru9I TTAA 2 cut(s) 474, 1512
TscAI CASTG 6 cut(s) 175, 392, 840, 908, 1490, 1553
TseFI GTSAC 4 cut(s) 946, 1459, 1496, 1607
TseI GCWGC 7 cut(s) 390, 595, 775, 805, 1071, 1074, 1659
Tsp45I GTSAC 4 cut(s) 946, 1459, 1496, 1607
TspDTI ATGAA 4 cut(s) 590, 817, 1071, 1398
TspGWI ACGGA 2 cut(s) 138, 519
TspRI CASTG 6 cut(s) 175, 392, 840, 908, 1490, 1553
Van91I CCANNNNNTGG 1 cut(s) 277
VspI ATTAAT 1 cut(s) 1512
XceI RCATGY 4 cut(s) 376, 605, 657, 1570
XmiI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 3 cut(s) 1307, 1442, 1663
ZrmI AGTACT 1 cut(s) 565
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.