MD15G1042700.v1.1

Ubiquitinyl hydrolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012946
Physical Location & Seq
Reverse (-)
2978586 .. 2980795
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1042700.v1.1.491

Sequence Viewer

Length: 1665 bp
ATGTCGACGAGGAAGCTTTCATACACAAACCCAAAACCCTGCAACCACCTCTCAGCTTACAAGCTCAAACATGGCTGCAACGGCTACAAATCCCTCCAGAAATCCCTCAAATCCGCCCCGAACGGAAGAACAAGCGTTCGGATTCACGAAACAAAGATACCCAGATGCAGTTTCTGTAATGGGTTCGAAGGTAGACTCTATGTTTGCTTGATTTGTTCTGCGGTTTCGTGTTCGAATCACGCAGTTCTGCATACCCAGTTGGAAATCGGCCACGAGTTGGCCGTCGATGTCGAAAGGGCCGAGCTTTTTTGCTGTGCGTGTACCGATCAGGTCTACGATCCCGATTTCGATAAGTCTGTCATGGCTAAGCACATGCTGGACTTGCCTTGCAGCACAAACAATGCGGATAGTATAGGGGAGAGACTGTGTGAGAGGAAGAGGCTGGGGGGTTCGGGTGTCGAATTATCAGAGTTTAAAAAGCCGGAAAAATCGTGTTTGGTGAGGGATCACAGAGCGAAATCGTGTTATCCGTTGGGTTTGAGGGGGTTGAATAATTTGGGGAATACTTGCTTTATGAACTCTGTGTTGCAAGCACTGCTACATGCGCCTCCTTTGAGGAATTACTTCTTGAGTGATCGGCATAGCAGGAGAACGTGCCAGAAAAGGACGGCAAACCGGCTTTGTTTGCCTTGTGAGCTTCACGGTGTTTATTCGGCTGTGTATTCGGGCGATCAGACTCCTTATAGCCCGGCTGAATTTCTTTACAGTTGGTGGCAGCATTCGGAGAATCTGGCAAGTTACGAGCAGCAGGACGCTCATGAGTTCTTCATTTCAGTGTTAGATGGGATCCATGAGAAAGAAACCAAAGCAAGGAACCATACGAAAGATAGTGGAGACTGCCAATGTATTGCGCATAGGGTATTCTCAGGACTGTTGAGGTCGGATGTCACTTGTATGACTTGTGGATTCACCTCGACAACTTATGACCCGTGTTTGGACATATCACTCAACTTAGACACAAGCCACTGTTCTTGGTCAGATGCATCTAACAAGCCTGCCAAAGCGAATGGCAGCAGCAGTTCATCCACTCTTTTGGGTTGTTTAGACTTGTTTACAAAACCAGAAAAGTTGGGGTCAGACCAGAAACTGTACTGTCAGAACTGTCAAGAACCCCGAGACTCTTCGAAGCAAATGTCCATCAGAAAGCTTCCAATGGTGTTGTGTTTGCATATAAAACGGTTTGAGCACTCCCTGGTCAGAAAGATGATGAGGAAAATCGACAGCTATTTGCATTTTCCATTCTCGCTAGACATGACTCCCTATCTGTCTTCTTCGATTGTGAGGAACAGATTTGGTAATAGAATCTTTGCTTTCGAGGGGGATGAGTCTGGGATGTCTACTGAGTTTGAGATTTTTGCAGTAGTTACTCATTCTGGGACACTAGATTCCGGCCATTATGTGACGTATTTACGCATACAAGACCAATGGTACAAATGTGACAATGCTTGGATTAATGAGGTCGACGAGGGGTTTGTAAGAGCTTCACAGTGTTATATGATGTTCTATGTGCACAAGACGCTTTACTATAAAGCAAACATAGATTTGCGTCACTTGCCGGTTTCCACACAGAAGGAGGCATTTCAGAGGATGGCTGGTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

555

Amino Acids

62.88

Weight (kDa)

8.35

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 56 - 115 5.7e-10 Zn-finger in ubiquitin-hydrolases and other protein
UCH PF00443 181 - 522 5.7e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 182 - 497 2.8e-20 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10790 AT5G10790
fragaria_vesca FvH4_2g41240
malus_domestica MD08G1052900.v1.1 MD15G1042700.v1.1
prunus_persica Prupe.1G396000_v2.0.a1
pyrus_communis pycom08g04410 pycom15g03980
rosa_chinensis RchiOBHm_Chr6g0299261
rosa_laevigata RLG00000011448
rosa_multiflora Rmu_sc0001434.1_g000005
rosa_roxburghii Rroxscaffold_7G00168850
rosa_rugosa Rorug06G0286700
rosa_samantha Rh6AG398000 Rh6BG406500 Rh6CG412600 Rh6DG399300
rosa_wichuraiana Rw6G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 912
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
AciI CCGC 3 cut(s) 114, 221, 404
AclWI GGATC 4 cut(s) 332, 513, 841, 854
AcoI YGGCCR 3 cut(s) 268, 279, 1450
AcsI RAATTY 1 cut(s) 755
AfaI GTAC 3 cut(s) 322, 1151, 1490
AfiI CCNNNNNNNGG 3 cut(s) 277, 870, 994
AgsI TTSAA 1 cut(s) 550
AjnI CCWGG 1 cut(s) 1251
AluBI AGCT 8 cut(s) 16, 56, 64, 304, 697, 1207, 1284, 1541
AluI AGCT 8 cut(s) 16, 56, 64, 304, 697, 1207, 1284, 1541
Alw21I GWGCWC 2 cut(s) 1248, 1572
Alw26I GTCTC 3 cut(s) 415, 888, 1170
Alw44I GTGCAC 1 cut(s) 1568
AlwI GGATC 4 cut(s) 332, 513, 841, 854
AlwNI CAGNNNCTG 2 cut(s) 174, 1147
Ama87I CYCGRG 1 cut(s) 1173
AoxI GGCC 4 cut(s) 268, 279, 297, 1450
ApaLI GTGCAC 1 cut(s) 1568
ApeKI GCWGC 7 cut(s) 75, 390, 775, 805, 1071, 1074, 1659
ApoI RAATTY 1 cut(s) 755
ArsI GACNNNNNNTTYG 6 cut(s) 186, 218, 1178, 1210, 1514, 1546
AseI ATTAAT 1 cut(s) 1512
Asp700I GAANNNNTTC 1 cut(s) 623
AspLEI GCGC 2 cut(s) 607, 913
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 1 cut(s) 749
AsuHPI GGTGA 2 cut(s) 511, 961
AsuII TTCGAA 3 cut(s) 186, 233, 1184
AvaI CYCGRG 1 cut(s) 1173
BaeGI GKGCMC 1 cut(s) 1572
BaeI ACNNNNGTAYC 2 cut(s) 1480, 1513
BamHI GGATCC 1 cut(s) 846
BarI GAAGNNNNNNTAC 1 cut(s) 37
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1320
Bbv12I GWGCWC 2 cut(s) 1248, 1572
BbvI GCAGC 7 cut(s) 62, 402, 787, 817, 1083, 1086, 1646
BccI CCATC 3 cut(s) 836, 1205, 1642
BceAI ACGGC 3 cut(s) 97, 266, 684
BciT130I CCWGG 1 cut(s) 1253
BcnI CCSGG 1 cut(s) 749
BcoDI GTCTC 3 cut(s) 415, 888, 1170
BfaI CTAG 2 cut(s) 1307, 1442
BisI GCNGC 7 cut(s) 76, 391, 776, 806, 1072, 1075, 1660
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 7 cut(s) 77, 392, 777, 807, 1073, 1076, 1661
Bme1390I CCNGG 2 cut(s) 749, 1253
BmeT110I CYCGRG 1 cut(s) 1173
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 2 cut(s) 848, 875
BmrFI CCNGG 2 cut(s) 749, 1253
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 3 cut(s) 155, 1030, 1052
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 1320
BpmI CTGGAG 1 cut(s) 80
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 3 cut(s) 186, 233, 1184
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 1 cut(s) 749
BsaJI CCNNGG 1 cut(s) 1251
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 870, 994
Bse118I RCCGGY 2 cut(s) 675, 1615
Bse1I ACTGG 1 cut(s) 256
BseBI CCWGG 1 cut(s) 1253
BseDI CCNNGG 1 cut(s) 1251
BseGI GGATG 5 cut(s) 949, 1082, 1387, 1398, 1653
BseLI CCNNNNNNNGG 3 cut(s) 277, 870, 994
BseMII CTCAG 3 cut(s) 66, 939, 1392
BseNI ACTGG 1 cut(s) 256
BseSI GKGCMC 1 cut(s) 1572
BseXI GCAGC 7 cut(s) 62, 402, 787, 817, 1083, 1086, 1646
BseYI CCCAGC 1 cut(s) 442
BshFI GGCC 4 cut(s) 270, 281, 299, 1452
BsiHKAI GWGCWC 2 cut(s) 1248, 1572
BsiHKCI CYCGRG 1 cut(s) 1173
BsiSI CCGG 5 cut(s) 482, 676, 749, 1449, 1616
BslFI GGGAC 1 cut(s) 1450
BslI CCNNNNNNNGG 3 cut(s) 277, 870, 994
BsmAI GTCTC 3 cut(s) 415, 888, 1170
BsmFI GGGAC 1 cut(s) 1450
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 4 cut(s) 270, 281, 299, 1452
BsoBI CYCGRG 1 cut(s) 1173
Bsp119I TTCGAA 3 cut(s) 186, 233, 1184
Bsp1286I GDGCHC 2 cut(s) 1248, 1572
Bsp143I GATC 6 cut(s) 325, 337, 505, 634, 730, 846
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 3 cut(s) 114, 221, 404
BspANI GGCC 4 cut(s) 270, 281, 299, 1452
BspCNI CTCAG 3 cut(s) 65, 938, 1393
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 2 cut(s) 848, 875
BspPI GGATC 4 cut(s) 332, 513, 841, 854
BspT104I TTCGAA 3 cut(s) 186, 233, 1184
BsrFI RCCGGY 2 cut(s) 675, 1615
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 2 cut(s) 675, 1615
BssECI CCNNGG 1 cut(s) 1251
BssMI GATC 6 cut(s) 325, 337, 505, 634, 730, 846
BssSI CACGAG 1 cut(s) 272
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 1 cut(s) 1253
Bst6I CTCTTC 2 cut(s) 431, 1186
BstAPI GCANNNNNTGC 1 cut(s) 595
BstBI TTCGAA 3 cut(s) 186, 233, 1184
BstC8I GCNNGC 2 cut(s) 591, 1056
BstDEI CTNAG 5 cut(s) 52, 366, 925, 1012, 1401
BstF5I GGATG 5 cut(s) 949, 1082, 1387, 1398, 1653
BstHHI GCGC 2 cut(s) 607, 913
BstKTI GATC 6 cut(s) 328, 340, 508, 637, 733, 849
BstMAI GTCTC 3 cut(s) 415, 888, 1170
BstMBI GATC 6 cut(s) 325, 337, 505, 634, 730, 846
BstMWI GCNNNNNNNGC 8 cut(s) 81, 382, 595, 604, 685, 694, 1576, 1612
BstNI CCWGG 1 cut(s) 1253
BstNSI RCATGY 2 cut(s) 376, 605
BstSCI CCNGG 2 cut(s) 747, 1251
BstSLI GKGCMC 1 cut(s) 1572
BstV1I GCAGC 7 cut(s) 62, 402, 787, 817, 1083, 1086, 1646
BstV2I GAAGAC 1 cut(s) 1320
BstX2I RGATCY 1 cut(s) 846
BstXI CCANNNNNNTGG 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 846
BsuRI GGCC 4 cut(s) 270, 281, 299, 1452
BtsCI GGATG 5 cut(s) 949, 1082, 1387, 1398, 1653
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 4 cut(s) 593, 840, 1024, 1553
Cac8I GCNNGC 2 cut(s) 591, 1056
CaiI CAGNNNCTG 2 cut(s) 174, 1147
CciI TCATGA 1 cut(s) 817
CfoI GCGC 2 cut(s) 607, 913
Cfr10I RCCGGY 2 cut(s) 675, 1615
Cfr13I GGNCC 1 cut(s) 297
CseI GACGC 3 cut(s) 821, 1585, 1595
Csp6I GTAC 3 cut(s) 321, 1150, 1489
CspCI CAANNNNNGTGG 2 cut(s) 1013, 1048
CviAII CATG 7 cut(s) 71, 361, 373, 602, 818, 851, 1312
CviQI GTAC 3 cut(s) 321, 1150, 1489
DdeI CTNAG 5 cut(s) 52, 366, 925, 1012, 1401
DpnI GATC 6 cut(s) 327, 339, 507, 636, 732, 848
DpnII GATC 6 cut(s) 325, 337, 505, 634, 730, 846
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 3 cut(s) 268, 279, 1450
Eam1104I CTCTTC 2 cut(s) 431, 1186
EarI CTCTTC 2 cut(s) 431, 1186
EciI GGCGGA 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 1173
EcoRII CCWGG 1 cut(s) 1251
EcoT22I ATGCAT 1 cut(s) 1045
FaeI CATG 7 cut(s) 74, 364, 376, 605, 821, 854, 1315
FaqI GGGAC 1 cut(s) 1450
FatI CATG 7 cut(s) 70, 360, 372, 601, 817, 850, 1311
FblI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
Fnu4HI GCNGC 7 cut(s) 76, 391, 776, 806, 1072, 1075, 1660
FokI GGATG 5 cut(s) 956, 1069, 1394, 1405, 1660
Fsp4HI GCNGC 7 cut(s) 76, 391, 776, 806, 1072, 1075, 1660
FspBI CTAG 2 cut(s) 1307, 1442
FspI TGCGCA 1 cut(s) 912
GlaI GCGC 2 cut(s) 606, 912
GluI GCNGC 7 cut(s) 76, 391, 776, 806, 1072, 1075, 1660
GsaI CCCAGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 4 cut(s) 270, 281, 299, 1452
HapII CCGG 5 cut(s) 482, 676, 749, 1449, 1616
HgaI GACGC 3 cut(s) 821, 1585, 1595
HhaI GCGC 2 cut(s) 607, 913
Hin1II CATG 7 cut(s) 74, 364, 376, 605, 821, 854, 1315
Hin6I GCGC 2 cut(s) 605, 911
HinP1I GCGC 2 cut(s) 605, 911
HincII GTYRAC 2 cut(s) 6, 1522
HindII GTYRAC 2 cut(s) 6, 1522
HindIII AAGCTT 2 cut(s) 14, 1205
HpaII CCGG 5 cut(s) 482, 676, 749, 1449, 1616
HphI GGTGA 2 cut(s) 511, 961
Hpy166II GTNNAC 8 cut(s) 6, 194, 321, 334, 1113, 1398, 1522, 1570
Hpy188III TCNNGA 7 cut(s) 97, 146, 341, 628, 818, 927, 1166
Hpy8I GTNNAC 8 cut(s) 6, 194, 321, 334, 1113, 1398, 1522, 1570
Hpy99I CGWCG 3 cut(s) 10, 287, 1526
HpyAV CCTTC 2 cut(s) 182, 1624
HpyCH4IV ACGT 2 cut(s) 653, 1463
HpyF10VI GCNNNNNNNGC 8 cut(s) 81, 382, 595, 604, 685, 694, 1576, 1612
HpyF3I CTNAG 5 cut(s) 52, 366, 925, 1012, 1401
HpySE526I ACGT 2 cut(s) 653, 1463
Hsp92II CATG 7 cut(s) 74, 364, 376, 605, 821, 854, 1315
HspAI GCGC 2 cut(s) 605, 911
Kzo9I GATC 6 cut(s) 325, 337, 505, 634, 730, 846
Lsp1109I GCAGC 7 cut(s) 62, 402, 787, 817, 1083, 1086, 1646
LweI GCATC 3 cut(s) 155, 1030, 1052
MaeI CTAG 2 cut(s) 1307, 1442
MaeII ACGT 2 cut(s) 653, 1463
MaeIII GTNAC 6 cut(s) 797, 946, 1423, 1459, 1496, 1607
MalI GATC 6 cut(s) 327, 339, 507, 636, 732, 848
MboI GATC 6 cut(s) 325, 337, 505, 634, 730, 846
MboII GAAGA 6 cut(s) 138, 448, 817, 1173, 1320, 1323
MflI RGATCY 1 cut(s) 846
MhlI GDGCHC 2 cut(s) 1248, 1572
MluCI AATT 4 cut(s) 461, 553, 619, 755
MlyI GAGTC 5 cut(s) 189, 730, 1172, 1309, 1394
MmeI TCCRAC 2 cut(s) 240, 921
Mph1103I ATGCAT 1 cut(s) 1045
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 2 cut(s) 474, 1512
MslI CAYNNNNRTG 2 cut(s) 833, 953
MspI CCGG 5 cut(s) 482, 676, 749, 1449, 1616
MspR9I CCNGG 2 cut(s) 749, 1253
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 1 cut(s) 1253
MwoI GCNNNNNNNGC 8 cut(s) 81, 382, 595, 604, 685, 694, 1576, 1612
NciI CCSGG 1 cut(s) 749
NdeII GATC 6 cut(s) 325, 337, 505, 634, 730, 846
NlaIII CATG 7 cut(s) 74, 364, 376, 605, 821, 854, 1315
NlaIV GGNNCC 2 cut(s) 848, 875
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 4 cut(s) 946, 1459, 1496, 1607
NsbI TGCGCA 1 cut(s) 912
NsiI ATGCAT 1 cut(s) 1045
NspI RCATGY 2 cut(s) 376, 605
NspV TTCGAA 3 cut(s) 186, 233, 1184
PagI TCATGA 1 cut(s) 817
PcsI WCGNNNNNNNCGW 3 cut(s) 279, 297, 527
PctI GAATGC 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 6 cut(s) 142, 235, 787, 966, 1362, 1445
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 7 cut(s) 77, 392, 777, 807, 1073, 1076, 1661
PleI GAGTC 5 cut(s) 189, 730, 1172, 1309, 1393
PpsI GAGTC 5 cut(s) 189, 730, 1172, 1309, 1393
PshBI ATTAAT 1 cut(s) 1512
Psp6I CCWGG 1 cut(s) 1251
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 1251
PspN4I GGNNCC 2 cut(s) 848, 875
PspPI GGNCC 1 cut(s) 297
PstNI CAGNNNCTG 2 cut(s) 174, 1147
PsuI RGATCY 1 cut(s) 846
RsaI GTAC 3 cut(s) 322, 1151, 1490
RsaNI GTAC 3 cut(s) 321, 1150, 1489
RseI CAYNNNNRTG 2 cut(s) 833, 953
SalI GTCGAC 2 cut(s) 4, 1520
SaqAI TTAA 2 cut(s) 474, 1512
SatI GCNGC 7 cut(s) 76, 391, 776, 806, 1072, 1075, 1660
Sau3AI GATC 6 cut(s) 325, 337, 505, 634, 730, 846
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 5 cut(s) 189, 730, 1172, 1309, 1394
ScrFI CCNGG 2 cut(s) 749, 1253
SduI GDGCHC 2 cut(s) 1248, 1572
SfaNI GCATC 3 cut(s) 155, 1030, 1052
SfuI TTCGAA 3 cut(s) 186, 233, 1184
SmiMI CAYNNNNRTG 2 cut(s) 833, 953
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
Sse9I AATT 4 cut(s) 461, 553, 619, 755
SsiI CCGC 3 cut(s) 114, 221, 404
SspMI CTAG 2 cut(s) 1307, 1442
StyD4I CCNGG 2 cut(s) 747, 1251
TaiI ACGT 2 cut(s) 656, 1466
TasI AATT 4 cut(s) 461, 553, 619, 755
TatI WGTACW 1 cut(s) 1149
TfiI GAWTC 6 cut(s) 142, 235, 787, 966, 1362, 1445
Tru1I TTAA 2 cut(s) 474, 1512
Tru9I TTAA 2 cut(s) 474, 1512
TscAI CASTG 4 cut(s) 600, 840, 1031, 1553
TseFI GTSAC 4 cut(s) 946, 1459, 1496, 1607
TseI GCWGC 7 cut(s) 75, 390, 775, 805, 1071, 1074, 1659
Tsp45I GTSAC 4 cut(s) 946, 1459, 1496, 1607
TspDTI ATGAA 4 cut(s) 9, 590, 817, 1071
TspGWI ACGGA 2 cut(s) 138, 519
TspRI CASTG 4 cut(s) 600, 840, 1031, 1553
Van91I CCANNNNNTGG 1 cut(s) 277
VneI GTGCAC 1 cut(s) 1568
VspI ATTAAT 1 cut(s) 1512
XapI RAATTY 1 cut(s) 755
XceI RCATGY 2 cut(s) 376, 605
XmiI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 2 cut(s) 1307, 1442
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.