pycom08g04410

Ubiquitinyl hydrolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012946
Physical Location & Seq
Forward (+)
3387297 .. 3389503
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04410.2

Sequence Viewer

Length: 1665 bp
ATGTCGACAAGGAAGCCTTCATACGCAAACCCAAAACCCTGCAACCACCTCTCAGCTTACAAGCTCAAATATGGCTCCAGCGGCTACAAATCCCTCCAAAAATCCCTCAAATCTGCCCCCAACGGAAGAACAAGCGTTCGGATTCATGAAACAAAGATACCCAGATGCAGTTTCTGTAATGGGTTCGAAGGTAGACTCTATATCTGCTTGATTTGCTCTTCGGTTTCGTGTTCGAATCACGCAGTTCTGCATACCCAGTTGGAAATCGGCCACGATTTGGCCGTCGATGTCCAAAGGGCCGAGCTTTTTTGCTGTGCGTGCTCCGATCAGGTCTACGATCCCGATTTCGATAAGTCCGTGATGGCTAAGCACATGCTGGACTTGCCGTGCAGCACAAACAGTGCGGATAGTATTGGGGAGAGGCTGTGTGAGAGGAAGAGGCTGGGGGGTTCGGGTGTCGTATTATCGGAGTTTAAAAAGCCCGAAAAATCGGGTTTTGTGAGGGATCACACGGCGAAATCGTGTTATCCGTTGGGTTTGAGGGGGTTGAATAATTTGGGGAATACTTGTTTTATGAACTCTGTGTTGCAAGCACTGCTACATGCGCCTCCATTGAGGAATTACTTCTTGAGTGATCGGCATAGCCGCAGAACATGCCGGAAAAGGACGGCAAACCGGCTGTGTTTGCCTTGTGAGCTTCACGGTGTTTATTCGGCTGTGTATTCCGGCGATTGGACTCCTTATAGCCCGGCTCAATTGCTTTACAGTTGGTGGCAGCATTCGGAGAATCTGGCAAGTTACGAGCAGCAGGACGCTCATGAGTTCTTCATTTCAGTGTTAGATGGGATCCATGAGAAAGAAACCAAAGCAAGGAACCATACTAAAGATAGTGGAGACTGCCAGTGTATTGCGCATAGGGTATTCTCAGGACTGTTGAGGTCGGATGTCACTTGTATGACTTGTGGATTCACCTCGACAACTTATGACCCGTGTTTGGACATATCTCTCAACTTAGACACAAGCCGCTGTTCTTGGTCAGATGCATCTAACAAGCCTGCCAAAGTGAATGGCAGCAGCTGTTCATCCACTCTTTTGGGTTGTTTAGACTTGTTTACAAAACCAGAAAAGTTGGGGTCAGACCAGAAACTGTACTGTCAGAACTGTCAAGAACTCCGAGACTCTTTGAAGCAAATGTCCATCAGAAAGCTTCCAATGGTGTTGTGTTTGCATGTAAAACGGTTTGAGCACTCCCTGGTCAGAAAGATGATGAGGAAAATCGACAGCTATTTGCATTTTCCATTCTCGCTAGACATGACTCCCTATCTGTCCTCTTCGATTGTCAGAAACAGATTTGGAAATAGAATCTTTGCTTTCGAGGGGGATGAATCTGAGATGTCTACCGAGTTTGAGATTTTTGCAGTAGTTACTCATTCCGGGACACTAGATTCCGGCCATTATGTGACGTATTTACGCATACAAGACCAATGGTACAAATGTGACAATGCTTGGATTAATGAGGTCGACGAGGGGATTGTAAGAGCTTCGCAGTGTTATATGATGTTCTACGTGCACAAGACGCTGTACTATAAAGCAAACATAGATTTGCGTCACTTGCCGGTTTCCACACAGAAGGAAGCATTTCAGAGGATGGCTGGTTGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

555

Amino Acids

62.87

Weight (kDa)

8.55

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10790 AT5G10790
fragaria_vesca FvH4_2g41240
malus_domestica MD08G1052900.v1.1 MD15G1042700.v1.1
prunus_persica Prupe.1G396000_v2.0.a1
pyrus_communis pycom08g04410 pycom15g03980
rosa_chinensis RchiOBHm_Chr6g0299261
rosa_laevigata RLG00000011448
rosa_multiflora Rmu_sc0001434.1_g000005
rosa_roxburghii Rroxscaffold_7G00168850
rosa_rugosa Rorug06G0286700
rosa_samantha Rh6AG398000 Rh6BG406500 Rh6CG412600 Rh6DG399300
rosa_wichuraiana Rw6G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 912
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
AciI CCGC 4 cut(s) 81, 404, 646, 1024
AclWI GGATC 4 cut(s) 332, 513, 841, 854
AcoI YGGCCR 3 cut(s) 268, 279, 1450
AfaI GTAC 3 cut(s) 1151, 1490, 1583
AfiI CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
AgsI TTSAA 2 cut(s) 550, 1186
AjnI CCWGG 1 cut(s) 1251
AluBI AGCT 8 cut(s) 56, 64, 304, 697, 1077, 1207, 1284, 1541
AluI AGCT 8 cut(s) 56, 64, 304, 697, 1077, 1207, 1284, 1541
Alw21I GWGCWC 3 cut(s) 323, 1248, 1572
Alw26I GTCTC 2 cut(s) 888, 1170
Alw44I GTGCAC 1 cut(s) 1568
AlwI GGATC 4 cut(s) 332, 513, 841, 854
AlwNI CAGNNNCTG 3 cut(s) 174, 1077, 1147
AoxI GGCC 4 cut(s) 268, 279, 297, 1450
ApaLI GTGCAC 1 cut(s) 1568
ApeKI GCWGC 6 cut(s) 390, 775, 805, 1071, 1074, 1659
AseI ATTAAT 1 cut(s) 1512
Asp700I GAANNNNTTC 2 cut(s) 623, 1638
AspLEI GCGC 2 cut(s) 607, 913
AspS9I GGNCC 1 cut(s) 297
AsuC2I CCSGG 2 cut(s) 749, 1435
AsuHPI GGTGA 1 cut(s) 961
AsuII TTCGAA 2 cut(s) 186, 233
BaeGI GKGCMC 1 cut(s) 1572
BaeI ACNNNNGTAYC 2 cut(s) 1480, 1513
BamHI GGATCC 1 cut(s) 846
BarI GAAGNNNNNNTAC 1 cut(s) 37
Bbv12I GWGCWC 3 cut(s) 323, 1248, 1572
BbvI GCAGC 6 cut(s) 402, 787, 817, 1083, 1086, 1646
BccI CCATC 4 cut(s) 355, 836, 1205, 1642
BceAI ACGGC 4 cut(s) 266, 370, 528, 684
BciT130I CCWGG 1 cut(s) 1253
BcnI CCSGG 2 cut(s) 749, 1435
BcoDI GTCTC 2 cut(s) 888, 1170
BfaI CTAG 3 cut(s) 1307, 1442, 1663
BisI GCNGC 9 cut(s) 82, 391, 646, 776, 806, 1024, 1072, 1075, 1660
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 9 cut(s) 83, 392, 647, 777, 807, 1025, 1073, 1076, 1661
Bme1390I CCNGG 3 cut(s) 749, 1253, 1435
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 3 cut(s) 76, 848, 875
BmrFI CCNGG 3 cut(s) 749, 1253, 1435
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 3 cut(s) 155, 1030, 1052
BmuI ACTGGG 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 61
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 2 cut(s) 186, 233
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 2 cut(s) 749, 1435
BsaAI YACGTR 1 cut(s) 1567
BsaJI CCNNGG 1 cut(s) 1251
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
Bse118I RCCGGY 2 cut(s) 675, 1615
Bse1I ACTGG 2 cut(s) 256, 901
BseBI CCWGG 1 cut(s) 1253
BseDI CCNNGG 1 cut(s) 1251
BseGI GGATG 4 cut(s) 949, 1082, 1387, 1653
BseLI CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
BseMII CTCAG 3 cut(s) 66, 939, 1380
BseNI ACTGG 2 cut(s) 256, 901
BseSI GKGCMC 1 cut(s) 1572
BseXI GCAGC 6 cut(s) 402, 787, 817, 1083, 1086, 1646
BseYI CCCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 409
BshFI GGCC 4 cut(s) 270, 281, 299, 1452
BsiHKAI GWGCWC 3 cut(s) 323, 1248, 1572
BsiSI CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
BslFI GGGAC 1 cut(s) 1450
BslI CCNNNNNNNGG 4 cut(s) 277, 732, 870, 994
BsmAI GTCTC 2 cut(s) 888, 1170
BsmFI GGGAC 1 cut(s) 1450
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 4 cut(s) 270, 281, 299, 1452
Bsp119I TTCGAA 2 cut(s) 186, 233
Bsp1286I GDGCHC 3 cut(s) 323, 1248, 1572
Bsp143I GATC 5 cut(s) 325, 337, 505, 634, 846
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 4 cut(s) 81, 404, 646, 1024
BspANI GGCC 4 cut(s) 270, 281, 299, 1452
BspCNI CTCAG 3 cut(s) 65, 938, 1381
BspHI TCATGA 2 cut(s) 145, 817
BspLI GGNNCC 3 cut(s) 76, 848, 875
BspPI GGATC 4 cut(s) 332, 513, 841, 854
BspQI GCTCTTC 1 cut(s) 223
BspT104I TTCGAA 2 cut(s) 186, 233
BsrFI RCCGGY 2 cut(s) 675, 1615
BsrI ACTGG 2 cut(s) 256, 901
BssAI RCCGGY 2 cut(s) 675, 1615
BssECI CCNNGG 1 cut(s) 1251
BssMI GATC 5 cut(s) 325, 337, 505, 634, 846
Bst2UI CCWGG 1 cut(s) 1253
Bst4CI ACNGT 8 cut(s) 401, 704, 767, 933, 1149, 1154, 1163, 1239
Bst6I CTCTTC 3 cut(s) 223, 431, 1336
BstAPI GCANNNNNTGC 2 cut(s) 595, 654
BstBAI YACGTR 1 cut(s) 1567
BstBI TTCGAA 2 cut(s) 186, 233
BstC8I GCNNGC 3 cut(s) 319, 591, 1056
BstDEI CTNAG 5 cut(s) 52, 366, 925, 1012, 1389
BstF5I GGATG 4 cut(s) 949, 1082, 1387, 1653
BstHHI GCGC 2 cut(s) 607, 913
BstKTI GATC 5 cut(s) 328, 340, 508, 637, 849
BstMAI GTCTC 2 cut(s) 888, 1170
BstMBI GATC 5 cut(s) 325, 337, 505, 634, 846
BstNI CCWGG 1 cut(s) 1253
BstNSI RCATGY 4 cut(s) 376, 605, 657, 1232
BstSCI CCNGG 3 cut(s) 747, 1251, 1433
BstSLI GKGCMC 1 cut(s) 1572
BstV1I GCAGC 6 cut(s) 402, 787, 817, 1083, 1086, 1646
BstX2I RGATCY 1 cut(s) 846
BstXI CCANNNNNNTGG 1 cut(s) 1093
BstYI RGATCY 1 cut(s) 846
BsuRI GGCC 4 cut(s) 270, 281, 299, 1452
BtsCI GGATG 4 cut(s) 949, 1082, 1387, 1653
BtsI GCAGTG 2 cut(s) 593, 1553
BtsIMutI CAGTG 5 cut(s) 406, 593, 840, 908, 1553
Cac8I GCNNGC 3 cut(s) 319, 591, 1056
CaiI CAGNNNCTG 3 cut(s) 174, 1077, 1147
CciI TCATGA 2 cut(s) 145, 817
CfoI GCGC 2 cut(s) 607, 913
Cfr10I RCCGGY 2 cut(s) 675, 1615
Cfr13I GGNCC 1 cut(s) 297
CseI GACGC 3 cut(s) 821, 1585, 1595
Csp6I GTAC 3 cut(s) 1150, 1489, 1582
CviAII CATG 8 cut(s) 146, 373, 602, 654, 818, 851, 1229, 1312
CviQI GTAC 3 cut(s) 1150, 1489, 1582
DdeI CTNAG 5 cut(s) 52, 366, 925, 1012, 1389
DpnI GATC 5 cut(s) 327, 339, 507, 636, 848
DpnII GATC 5 cut(s) 325, 337, 505, 634, 846
DraI TTTAAA 1 cut(s) 475
EaeI YGGCCR 3 cut(s) 268, 279, 1450
Eam1104I CTCTTC 3 cut(s) 223, 431, 1336
EarI CTCTTC 3 cut(s) 223, 431, 1336
EcoRII CCWGG 1 cut(s) 1251
EcoT22I ATGCAT 1 cut(s) 1045
FaeI CATG 8 cut(s) 149, 376, 605, 657, 821, 854, 1232, 1315
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 1 cut(s) 1450
FatI CATG 8 cut(s) 145, 372, 601, 653, 817, 850, 1228, 1311
FblI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
Fnu4HI GCNGC 9 cut(s) 82, 391, 646, 776, 806, 1024, 1072, 1075, 1660
FokI GGATG 4 cut(s) 956, 1069, 1394, 1660
Fsp4HI GCNGC 9 cut(s) 82, 391, 646, 776, 806, 1024, 1072, 1075, 1660
FspBI CTAG 3 cut(s) 1307, 1442, 1663
FspI TGCGCA 1 cut(s) 912
GlaI GCGC 2 cut(s) 606, 912
GluI GCNGC 9 cut(s) 82, 391, 646, 776, 806, 1024, 1072, 1075, 1660
GsaI CCCAGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 61
HaeIII GGCC 4 cut(s) 270, 281, 299, 1452
HapII CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
HgaI GACGC 3 cut(s) 821, 1585, 1595
HhaI GCGC 2 cut(s) 607, 913
Hin1II CATG 8 cut(s) 149, 376, 605, 657, 821, 854, 1232, 1315
Hin6I GCGC 2 cut(s) 605, 911
HinP1I GCGC 2 cut(s) 605, 911
HincII GTYRAC 2 cut(s) 6, 1522
HindII GTYRAC 2 cut(s) 6, 1522
HindIII AAGCTT 1 cut(s) 1205
HpaII CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
HphI GGTGA 1 cut(s) 961
Hpy166II GTNNAC 7 cut(s) 6, 194, 334, 1113, 1398, 1522, 1570
Hpy188III TCNNGA 6 cut(s) 146, 341, 628, 818, 927, 1166
Hpy8I GTNNAC 7 cut(s) 6, 194, 334, 1113, 1398, 1522, 1570
Hpy99I CGWCG 2 cut(s) 287, 1526
HpyAV CCTTC 3 cut(s) 27, 182, 1624
HpyCH4III ACNGT 8 cut(s) 401, 704, 767, 933, 1149, 1154, 1163, 1239
HpyCH4IV ACGT 2 cut(s) 1463, 1566
HpyF3I CTNAG 5 cut(s) 52, 366, 925, 1012, 1389
HpySE526I ACGT 2 cut(s) 1463, 1566
Hsp92II CATG 8 cut(s) 149, 376, 605, 657, 821, 854, 1232, 1315
HspAI GCGC 2 cut(s) 605, 911
Kzo9I GATC 5 cut(s) 325, 337, 505, 634, 846
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 80, 326
Lsp1109I GCAGC 6 cut(s) 402, 787, 817, 1083, 1086, 1646
LweI GCATC 3 cut(s) 155, 1030, 1052
MaeI CTAG 3 cut(s) 1307, 1442, 1663
MaeII ACGT 2 cut(s) 1463, 1566
MaeIII GTNAC 6 cut(s) 797, 946, 1423, 1459, 1496, 1607
MalI GATC 5 cut(s) 327, 339, 507, 636, 848
MboI GATC 5 cut(s) 325, 337, 505, 634, 846
MboII GAAGA 5 cut(s) 138, 210, 448, 817, 1323
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 1 cut(s) 846
MhlI GDGCHC 3 cut(s) 323, 1248, 1572
MluCI AATT 3 cut(s) 553, 619, 755
MlyI GAGTC 4 cut(s) 189, 730, 1172, 1309
MmeI TCCRAC 2 cut(s) 240, 921
Mph1103I ATGCAT 1 cut(s) 1045
MroXI GAANNNNTTC 2 cut(s) 623, 1638
MseI TTAA 2 cut(s) 474, 1512
MslI CAYNNNNRTG 2 cut(s) 833, 953
MspA1I CMGCKG 3 cut(s) 81, 1026, 1077
MspI CCGG 7 cut(s) 658, 676, 726, 749, 1434, 1449, 1616
MspR9I CCNGG 3 cut(s) 749, 1253, 1435
MunI CAATTG 1 cut(s) 755
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 1 cut(s) 1253
NciI CCSGG 2 cut(s) 749, 1435
NdeII GATC 5 cut(s) 325, 337, 505, 634, 846
NlaIII CATG 8 cut(s) 149, 376, 605, 657, 821, 854, 1232, 1315
NlaIV GGNNCC 3 cut(s) 76, 848, 875
NmeAIII GCCGAG 1 cut(s) 325
NmuCI GTSAC 4 cut(s) 946, 1459, 1496, 1607
NsbI TGCGCA 1 cut(s) 912
NsiI ATGCAT 1 cut(s) 1045
NspI RCATGY 4 cut(s) 376, 605, 657, 1232
NspV TTCGAA 2 cut(s) 186, 233
PagI TCATGA 2 cut(s) 145, 817
PciSI GCTCTTC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 354, 518, 527
PctI GAATGC 1 cut(s) 778
PdmI GAANNNNTTC 2 cut(s) 623, 1638
PfeI GAWTC 7 cut(s) 142, 235, 787, 966, 1362, 1385, 1445
PflMI CCANNNNNTGG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 1433
PkrI GCNGC 9 cut(s) 83, 392, 647, 777, 807, 1025, 1073, 1076, 1661
PleI GAGTC 4 cut(s) 189, 730, 1172, 1309
PpsI GAGTC 4 cut(s) 189, 730, 1172, 1309
Ppu21I YACGTR 1 cut(s) 1567
PshBI ATTAAT 1 cut(s) 1512
Psp6I CCWGG 1 cut(s) 1251
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 1251
PspN4I GGNNCC 3 cut(s) 76, 848, 875
PspPI GGNCC 1 cut(s) 297
PstNI CAGNNNCTG 3 cut(s) 174, 1077, 1147
PsuI RGATCY 1 cut(s) 846
PvuII CAGCTG 1 cut(s) 1077
RsaI GTAC 3 cut(s) 1151, 1490, 1583
RsaNI GTAC 3 cut(s) 1150, 1489, 1582
RseI CAYNNNNRTG 2 cut(s) 833, 953
SalI GTCGAC 2 cut(s) 4, 1520
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 2 cut(s) 474, 1512
SatI GCNGC 9 cut(s) 82, 391, 646, 776, 806, 1024, 1072, 1075, 1660
Sau3AI GATC 5 cut(s) 325, 337, 505, 634, 846
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 4 cut(s) 189, 730, 1172, 1309
ScrFI CCNGG 3 cut(s) 749, 1253, 1435
SduI GDGCHC 3 cut(s) 323, 1248, 1572
SfaNI GCATC 3 cut(s) 155, 1030, 1052
SfuI TTCGAA 2 cut(s) 186, 233
SmiMI CAYNNNNRTG 2 cut(s) 833, 953
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
Sse9I AATT 3 cut(s) 553, 619, 755
SsiI CCGC 4 cut(s) 81, 404, 646, 1024
SspMI CTAG 3 cut(s) 1307, 1442, 1663
StyD4I CCNGG 3 cut(s) 747, 1251, 1433
TaaI ACNGT 8 cut(s) 401, 704, 767, 933, 1149, 1154, 1163, 1239
TaiI ACGT 2 cut(s) 1466, 1569
TasI AATT 3 cut(s) 553, 619, 755
TatI WGTACW 2 cut(s) 1149, 1581
TauI GCSGC 3 cut(s) 84, 648, 1026
TfiI GAWTC 7 cut(s) 142, 235, 787, 966, 1362, 1385, 1445
Tru1I TTAA 2 cut(s) 474, 1512
Tru9I TTAA 2 cut(s) 474, 1512
TscAI CASTG 5 cut(s) 406, 600, 840, 908, 1553
TseFI GTSAC 4 cut(s) 946, 1459, 1496, 1607
TseI GCWGC 6 cut(s) 390, 775, 805, 1071, 1074, 1659
Tsp45I GTSAC 4 cut(s) 946, 1459, 1496, 1607
TspDTI ATGAA 7 cut(s) 9, 134, 162, 590, 817, 1071, 1398
TspGWI ACGGA 3 cut(s) 138, 346, 519
TspRI CASTG 5 cut(s) 406, 600, 840, 908, 1553
Van91I CCANNNNNTGG 1 cut(s) 277
VneI GTGCAC 1 cut(s) 1568
VspI ATTAAT 1 cut(s) 1512
XceI RCATGY 4 cut(s) 376, 605, 657, 1232
XmiI GTMKAC 5 cut(s) 5, 193, 333, 1397, 1521
XmnI GAANNNNTTC 2 cut(s) 623, 1638
XspI CTAG 3 cut(s) 1307, 1442, 1663
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.