Rorug06G0286700

Folate-Biopterin Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0008245
Physical Location & Seq
Reverse (-)
43685500 .. 43686550
1051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0286700.1

Sequence Viewer

Length: 447 bp
ATGTCAAACAATGGGAACCCTGCAGAAAACGGTAACGGTGGGAATCCGGATAGGCCAGCTTTGAGGAATTGGAGAAACAAGCTGACTATCCTTCTTATTGACGAATCTGAAAACCACAGGATTCTGGGGACGGCTTATCTTACAGCTTATGGTGTCGAAACTACATCTGTGACTGATGGACGGGCTGCTTTGGAGCTCATATTTTCTGGTGCTAGGTTCAGTTTGATCCTCATTGACATGCATATGCGCTATATGAGCGGTGCACAGTGTGTGAGTCACCTTCGAACCATGGGAGTGAGGTCCCTGATGCTCGGTATGACAACTTTTTGTAGAGAAAGAGAGGAACGGGCATTTCTGGAAGCAGGGGGTAATGGCTTCATTCCAAAGCCTCTTAGTCCTGATAACTTTCTTCAAGTCCTGGAAGCGCTGGATAACAACCAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

148

Amino Acids

16.33

Weight (kDa)

5.9

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 30 - 140 4.1e-14 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 1 cut(s) 258
AclWI GGATC 1 cut(s) 220
AdeI CACNNNGTG 1 cut(s) 269
AfeI AGCGCT 1 cut(s) 426
AgsI TTSAA 1 cut(s) 413
AjnI CCWGG 2 cut(s) 417, 438
AluBI AGCT 4 cut(s) 59, 82, 146, 196
AluI AGCT 4 cut(s) 59, 82, 146, 196
Alw21I GWGCWC 2 cut(s) 198, 265
Alw44I GTGCAC 1 cut(s) 261
AlwI GGATC 1 cut(s) 220
Aor13HI TCCGGA 1 cut(s) 46
Aor51HI AGCGCT 1 cut(s) 426
AoxI GGCC 1 cut(s) 53
ApaLI GTGCAC 1 cut(s) 261
ApeKI GCWGC 1 cut(s) 185
AspLEI GCGC 2 cut(s) 249, 427
AspS9I GGNCC 1 cut(s) 300
AsuHPI GGTGA 1 cut(s) 269
AsuII TTCGAA 1 cut(s) 283
AvaII GGWCC 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 198, 265
BbvI GCAGC 1 cut(s) 172
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 147
BciT130I CCWGG 2 cut(s) 419, 440
BfaI CTAG 1 cut(s) 213
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 428
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 2 cut(s) 419, 440
Bme18I GGWCC 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 17, 302
BmrFI CCNGG 2 cut(s) 419, 440
BmsI GCATC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 283
BsaJI CCNNGG 2 cut(s) 288, 439
BsaWI WCCGGW 1 cut(s) 46
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 2 cut(s) 419, 440
BseDI CCNNGG 2 cut(s) 288, 439
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 55
BsiHKAI GWGCWC 2 cut(s) 198, 265
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 142, 286
BsmFI GGGAC 2 cut(s) 142, 286
BsnI GGCC 1 cut(s) 55
Bsp119I TTCGAA 1 cut(s) 283
Bsp1286I GDGCHC 2 cut(s) 198, 265
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 1 cut(s) 225
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 55
BspEI TCCGGA 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 17, 302
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 258
BssECI CCNNGG 2 cut(s) 288, 439
BssMI GATC 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 2 cut(s) 419, 440
Bst4CI ACNGT 3 cut(s) 32, 38, 267
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 1 cut(s) 392
BstDSI CCRYGG 1 cut(s) 288
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 2 cut(s) 249, 427
BstKTI GATC 1 cut(s) 228
BstMBI GATC 1 cut(s) 225
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstNI CCWGG 2 cut(s) 419, 440
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 2 cut(s) 417, 438
BstSFI CTRYAG 1 cut(s) 21
BstSLI GKGCMC 1 cut(s) 265
BstV1I GCAGC 1 cut(s) 172
BsuRI GGCC 1 cut(s) 55
BtgI CCRYGG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 57
CfoI GCGC 2 cut(s) 249, 427
Cfr13I GGNCC 1 cut(s) 300
CviAII CATG 2 cut(s) 238, 289
CviJI RGCY 9 cut(s) 55, 59, 82, 134, 146, 185, 196, 375, 388
CviKI_1 RGCY 9 cut(s) 55, 59, 82, 134, 146, 185, 196, 375, 388
DdeI CTNAG 1 cut(s) 392
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
DraIII CACNNNGTG 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 300
Eco47III AGCGCT 1 cut(s) 426
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 300
EcoRII CCWGG 2 cut(s) 417, 438
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 2 cut(s) 241, 292
FaiI YATR 9 cut(s) 150, 200, 239, 243, 245, 252, 254, 290, 317
FaqI GGGAC 2 cut(s) 142, 286
FatI CATG 2 cut(s) 237, 288
FauNDI CATATG 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 1 cut(s) 213
GlaI GCGC 2 cut(s) 248, 426
GluI GCNGC 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 1 cut(s) 55
HapII CCGG 1 cut(s) 47
HhaI GCGC 2 cut(s) 249, 427
Hin1II CATG 2 cut(s) 241, 292
Hin6I GCGC 2 cut(s) 247, 425
HinP1I GCGC 2 cut(s) 247, 425
HinfI GANTC 4 cut(s) 43, 104, 121, 274
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 3 cut(s) 47, 356, 398
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 101, 290
HpyCH4III ACNGT 3 cut(s) 32, 38, 267
HpyCH4V TGCA 3 cut(s) 23, 241, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpyF3I CTNAG 1 cut(s) 392
Hsp92II CATG 2 cut(s) 241, 292
HspAI GCGC 2 cut(s) 247, 425
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 1 cut(s) 297
MaeI CTAG 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 32, 169, 275
MalI GATC 1 cut(s) 227
MbiI CCGCTC 1 cut(s) 258
MboI GATC 1 cut(s) 225
MboII GAAGA 1 cut(s) 401
MhlI GDGCHC 2 cut(s) 198, 265
MluCI AATT 1 cut(s) 67
MlyI GAGTC 1 cut(s) 283
MnlI CCTC 5 cut(s) 57, 239, 291, 334, 399
Mph1103I ATGCAT 1 cut(s) 243
MroI TCCGGA 1 cut(s) 46
MslI CAYNNNNRTG 3 cut(s) 236, 242, 293
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 2 cut(s) 419, 440
MvaI CCWGG 2 cut(s) 419, 440
MwoI GCNNNNNNNGC 1 cut(s) 255
NcoI CCATGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 243
NdeII GATC 1 cut(s) 225
NlaIII CATG 2 cut(s) 241, 292
NlaIV GGNNCC 2 cut(s) 17, 302
NmuCI GTSAC 2 cut(s) 169, 275
NsiI ATGCAT 1 cut(s) 243
NspI RCATGY 1 cut(s) 241
NspV TTCGAA 1 cut(s) 283
PfeI GAWTC 3 cut(s) 43, 104, 121
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 282
PpuMI RGGWCCY 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 300
Psp6I CCWGG 2 cut(s) 417, 438
PspGI CCWGG 2 cut(s) 417, 438
PspN4I GGNNCC 2 cut(s) 17, 302
PspPI GGNCC 1 cut(s) 300
PspPPI RGGWCCY 1 cut(s) 300
PstI CTGCAG 1 cut(s) 25
RseI CAYNNNNRTG 3 cut(s) 236, 242, 293
SacI GAGCTC 1 cut(s) 198
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 1 cut(s) 300
SchI GAGTC 1 cut(s) 283
ScrFI CCNGG 2 cut(s) 419, 440
SduI GDGCHC 2 cut(s) 198, 265
SetI ASST 7 cut(s) 61, 84, 148, 198, 218, 282, 302
SfaNI GCATC 1 cut(s) 297
SfcI CTRYAG 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 1 cut(s) 300
SmiMI CAYNNNNRTG 3 cut(s) 236, 242, 293
Sse9I AATT 1 cut(s) 67
SsiI CCGC 1 cut(s) 258
SspMI CTAG 1 cut(s) 213
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 417, 438
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 3 cut(s) 32, 38, 267
TaqI TCGA 2 cut(s) 156, 283
TasI AATT 1 cut(s) 67
TfiI GAWTC 3 cut(s) 43, 104, 121
TscAI CASTG 1 cut(s) 272
TseFI GTSAC 2 cut(s) 169, 275
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 2 cut(s) 169, 275
TspDTI ATGAA 1 cut(s) 367
TspRI CASTG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 300
XceI RCATGY 1 cut(s) 241
XspI CTAG 1 cut(s) 213
Zsp2I ATGCAT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.