AT5G25040

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008245
Physical Location & Seq
Forward (+)
8621900 .. 8624484
2585 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25040.3

Sequence Viewer

Length: 1374 bp
ATGGCAAGAGAAGAGCAAAACATTGAAAACAGAGGATCCGTACATGTGGTCGAAAGAGAAAGTAATTTACGTAGTGTGGTGTGTGGTCCTGTGCGGTGGCTAAAGATGTTGGCGTCAAAGCTTCACTGGAGCTATGTGTTTGCTGTGGTCTCCAATTGTGGAATAAACCAAGGGTTTGGTTACTCATTGGGTCATGTAGCTACAGATTATTACATGAAAGATGTTCAAAAGGTTCAGCCTTCTCAATATCAAGCTCTCTCTGCAATCACTAGGATTTCATGGATCATATTTAAGCCTCTTTTTGGCATTCTCACTGATGTTCTTCCCATCTTTGGTTTCCATAGACGTCCTTACTTCATTTTAGCAGGGGTGATTGGAGTGGTTTCTTTGTTGTTTATATCACTTCAAAGTAATCTACATCTTTACTTGGCACTGTTTTGGATGACAATATCAAGTGCTGCAATGGCGATAGCCGATGTAACAATTGATGCTTGTACTACTTACAATAGTAACAAACACCCTTCTCTTGCATCGGATATGCAGAGTTTATGTAGTTTAAGCTATTCCATTGGAGAACTTCTTGGTTTCTTTATGAGTGGTATCTTAGTTCATCTTGTTGGCTCCAAGGGCGTGTTCGGCTTGTTAACATTCACATTCGCATTAGTATCGGTAGTCGGGGTCTTGTTTAGTGAGCCTCGTGTTCATGGTTTCTCTTTCAAACAGAACTTTACCAATGCAATGAAAGCGATGTGGAGGACAATCAAGTGCTCAGATGTGTGGCAGCCAAGTCTATATATGTTCATTACCCGTGCTCTTGGTTTAGACATTAAAGAAGGTCTTTTCTATTGGTTCACTGATTCAAAAGATGGTCCTTTGTTTGCTCAGGAAACGGTTGGTTTCATTCTCTCAATTGGTTCTATAGGGTCGATTCTAGGAGTACTGTTGTACAACCTACGCCTAAAGGATCATCCGTTCCGTAAACTATTTTTGTGGACTCAACTTCTCTTTGCTTTATCAGGAATGTTTGACCTCATTCTTGTGCTACGTCTCAACTTAAAATTTGGTTTACCTGATTATCTCTTCATAGTAGTGGACGGGATAGTTTCTAAGATGATCATACGTCTGACATGGATGGTGATATTCGTTCTCAATACAAAGCTTTGTCCTCACGGAATTGAAGGGACCTTTTTTGCGTTGTTAATGTCAATTGACAATGCAGGCCTCATGACCTCATCTTGGCTTGGAGGGAAAATGTTGCATGTCCTCAAGAACACGTTCAAGCTCCCGGCTGAGATAATGGGCGAAGATTCGGAAGAAGAGGGAACTCGGAATTTGGAATTGGCTTCTCTTGTTGACTATGTTGATAGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

457

Amino Acids

51.29

Weight (kDa)

8.41

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BT1 PF03092 57 - 378 5.8e-77 BT1 family
MFS_1 PF07690 67 - 338 3.4e-08 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 349
AciI CCGC 1 cut(s) 94
AclWI GGATC 4 cut(s) 30, 43, 290, 972
AcsI RAATTY 2 cut(s) 1058, 1330
AcyI GRCGYC 2 cut(s) 113, 346
AfaI GTAC 4 cut(s) 42, 496, 939, 947
AfiI CCNNNNNNNGG 3 cut(s) 302, 332, 1236
AflIII ACRYGT 2 cut(s) 43, 1272
AgsI TTSAA 7 cut(s) 26, 227, 407, 718, 861, 1178, 1279
AjuI GAANNNNNNNTTGG 4 cut(s) 617, 649, 1322, 1354
AloI GAACNNNNNNTCC 2 cut(s) 956, 988
AluBI AGCT 8 cut(s) 121, 132, 200, 254, 561, 1159, 1282, 1371
AluI AGCT 8 cut(s) 121, 132, 200, 254, 561, 1159, 1282, 1371
Alw21I GWGCWC 2 cut(s) 770, 814
Alw26I GTCTC 2 cut(s) 154, 1052
AlwI GGATC 4 cut(s) 30, 43, 290, 972
AoxI GGCC 1 cut(s) 1219
ApeKI GCWGC 2 cut(s) 458, 781
ApoI RAATTY 2 cut(s) 1058, 1330
Asp700I GAANNNNTTC 1 cut(s) 1274
AspS9I GGNCC 3 cut(s) 86, 869, 1182
AsuC2I CCSGG 1 cut(s) 1286
AsuHPI GGTGA 2 cut(s) 382, 1147
AvaII GGWCC 3 cut(s) 86, 869, 1182
BamHI GGATCC 1 cut(s) 35
BauI CACGAG 1 cut(s) 696
Bbv12I GWGCWC 2 cut(s) 770, 814
BbvI GCAGC 2 cut(s) 445, 793
BccI CCATC 3 cut(s) 335, 860, 1126
BcgI CGANNNNNNTGC 2 cut(s) 648, 682
BclI TGATCA 1 cut(s) 1113
BcnI CCSGG 1 cut(s) 1286
BcoDI GTCTC 2 cut(s) 154, 1052
BfaI CTAG 3 cut(s) 270, 932, 1372
BfmI CTRYAG 2 cut(s) 201, 918
BisI GCNGC 2 cut(s) 459, 782
BlsI GCNGC 2 cut(s) 460, 783
BmcAI AGTACT 1 cut(s) 939
Bme1390I CCNGG 1 cut(s) 1286
Bme18I GGWCC 3 cut(s) 86, 869, 1182
BmgT120I GGNCC 3 cut(s) 86, 869, 1182
BmiI GGNNCC 3 cut(s) 37, 622, 1183
BmrFI CCNGG 1 cut(s) 1286
BmsI GCATC 2 cut(s) 478, 539
BpmI CTGGAG 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 882
BpuEI CTTGAG 1 cut(s) 1250
BpuMI CCSGG 1 cut(s) 1286
BsaAI YACGTR 1 cut(s) 71
BsaHI GRCGYC 2 cut(s) 113, 346
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 2 cut(s) 169, 624
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 332, 1236
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 2 cut(s) 468, 744
BseDI CCNNGG 2 cut(s) 169, 624
BseGI GGATG 3 cut(s) 447, 967, 1137
BseLI CCNNNNNNNGG 3 cut(s) 302, 332, 1236
BseMI GCAATG 2 cut(s) 468, 744
BseMII CTCAG 3 cut(s) 783, 896, 1281
BseNI ACTGG 1 cut(s) 131
BseXI GCAGC 2 cut(s) 445, 793
BshFI GGCC 1 cut(s) 1221
BsiHKAI GWGCWC 2 cut(s) 770, 814
BsiSI CCGG 1 cut(s) 1286
BslFI GGGAC 1 cut(s) 1195
BslI CCNNNNNNNGG 3 cut(s) 302, 332, 1236
BsmAI GTCTC 2 cut(s) 154, 1052
BsmBI CGTCTC 1 cut(s) 1052
BsmFI GGGAC 1 cut(s) 1195
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 1 cut(s) 1221
Bso31I GGTCTC 1 cut(s) 154
Bsp1286I GDGCHC 2 cut(s) 770, 814
Bsp1407I TGTACA 1 cut(s) 945
Bsp143I GATC 4 cut(s) 35, 282, 964, 1113
BspACI CCGC 1 cut(s) 94
BspANI GGCC 1 cut(s) 1221
BspCNI CTCAG 3 cut(s) 782, 895, 1282
BspHI TCATGA 1 cut(s) 1224
BspLI GGNNCC 3 cut(s) 37, 622, 1183
BspPI GGATC 4 cut(s) 30, 43, 290, 972
BspQI GCTCTTC 1 cut(s) 6
BspTNI GGTCTC 1 cut(s) 154
BsrDI GCAATG 2 cut(s) 468, 744
BsrGI TGTACA 1 cut(s) 945
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 169, 624
BssMI GATC 4 cut(s) 35, 282, 964, 1113
BssNI GRCGYC 2 cut(s) 113, 346
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 2 cut(s) 169, 624
Bst2BI CACGAG 1 cut(s) 696
Bst4CI ACNGT 3 cut(s) 435, 892, 942
Bst6I CTCTTC 3 cut(s) 6, 1085, 1311
BstACI GRCGYC 2 cut(s) 113, 346
BstAUI TGTACA 1 cut(s) 945
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 1219
BstDEI CTNAG 5 cut(s) 604, 769, 882, 1107, 1290
BstF5I GGATG 3 cut(s) 447, 967, 1137
BstKTI GATC 4 cut(s) 38, 285, 967, 1116
BstMAI GTCTC 2 cut(s) 154, 1052
BstMBI GATC 4 cut(s) 35, 282, 964, 1113
BstMWI GCNNNNNNNGC 5 cut(s) 260, 464, 627, 636, 743
BstNSI RCATGY 2 cut(s) 47, 1262
BstSCI CCNGG 1 cut(s) 1284
BstSFI CTRYAG 2 cut(s) 201, 918
BstSNI TACGTA 1 cut(s) 71
BstV1I GCAGC 2 cut(s) 445, 793
BstX2I RGATCY 1 cut(s) 35
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 1 cut(s) 35
BsuRI GGCC 1 cut(s) 1221
BtgZI GCGATG 1 cut(s) 761
BtsCI GGATG 3 cut(s) 447, 967, 1137
BtsIMutI CAGTG 4 cut(s) 124, 312, 431, 852
Cac8I GCNNGC 1 cut(s) 1219
CciI TCATGA 1 cut(s) 1224
Cfr13I GGNCC 3 cut(s) 86, 869, 1182
CseI GACGC 1 cut(s) 102
Csp6I GTAC 4 cut(s) 41, 495, 938, 946
CviAII CATG 8 cut(s) 44, 194, 214, 279, 704, 1128, 1225, 1259
CviQI GTAC 4 cut(s) 41, 495, 938, 946
DdeI CTNAG 5 cut(s) 604, 769, 882, 1107, 1290
DpnI GATC 4 cut(s) 37, 284, 966, 1115
DpnII GATC 4 cut(s) 35, 282, 964, 1113
Eam1104I CTCTTC 3 cut(s) 6, 1085, 1311
EarI CTCTTC 3 cut(s) 6, 1085, 1311
Eco105I TACGTA 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 169, 624
Eco147I AGGCCT 1 cut(s) 1221
Eco31I GGTCTC 1 cut(s) 154
Eco47I GGWCC 3 cut(s) 86, 869, 1182
EcoO109I RGGNCCY 1 cut(s) 1182
EcoT14I CCWWGG 2 cut(s) 169, 624
ErhI CCWWGG 2 cut(s) 169, 624
Esp3I CGTCTC 1 cut(s) 1052
FaeI CATG 8 cut(s) 47, 197, 217, 282, 707, 1131, 1228, 1262
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 1 cut(s) 1195
FatI CATG 8 cut(s) 43, 193, 213, 278, 703, 1127, 1224, 1258
FbaI TGATCA 1 cut(s) 1113
Fnu4HI GCNGC 2 cut(s) 459, 782
FokI GGATG 3 cut(s) 454, 954, 1144
Fsp4HI GCNGC 2 cut(s) 459, 782
FspBI CTAG 3 cut(s) 270, 932, 1372
GluI GCNGC 2 cut(s) 459, 782
GsuI CTGGAG 1 cut(s) 148
HaeIII GGCC 1 cut(s) 1221
HapII CCGG 1 cut(s) 1286
HgaI GACGC 1 cut(s) 102
Hin1I GRCGYC 2 cut(s) 113, 346
Hin1II CATG 8 cut(s) 47, 197, 217, 282, 707, 1131, 1228, 1262
HincII GTYRAC 2 cut(s) 645, 1354
HindII GTYRAC 2 cut(s) 645, 1354
HindIII AAGCTT 2 cut(s) 119, 1157
HinfI GANTC 4 cut(s) 857, 928, 994, 1307
HpaI GTTAAC 1 cut(s) 645
HpaII CCGG 1 cut(s) 1286
HphI GGTGA 2 cut(s) 382, 1147
Hpy166II GTNNAC 7 cut(s) 645, 852, 980, 993, 1067, 1093, 1354
Hpy188I TCNGA 5 cut(s) 535, 772, 1125, 1312, 1329
Hpy188III TCNNGA 4 cut(s) 884, 1017, 1225, 1267
Hpy8I GTNNAC 7 cut(s) 645, 852, 980, 993, 1067, 1093, 1354
HpyAV CCTTC 4 cut(s) 249, 531, 827, 1172
HpyCH4III ACNGT 3 cut(s) 435, 892, 942
HpyCH4IV ACGT 5 cut(s) 70, 346, 1045, 1120, 1274
HpyCH4V TGCA 7 cut(s) 263, 461, 530, 541, 737, 1217, 1258
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 464, 627, 636, 743
HpyF3I CTNAG 5 cut(s) 604, 769, 882, 1107, 1290
HpySE526I ACGT 5 cut(s) 70, 346, 1045, 1120, 1274
Hsp92I GRCGYC 2 cut(s) 113, 346
Hsp92II CATG 8 cut(s) 47, 197, 217, 282, 707, 1131, 1228, 1262
Ksp22I TGATCA 1 cut(s) 1113
KspAI GTTAAC 1 cut(s) 645
Kzo9I GATC 4 cut(s) 35, 282, 964, 1113
LguI GCTCTTC 1 cut(s) 6
LmnI GCTCC 4 cut(s) 129, 626, 1287, 1368
LpnPI CCDG 8 cut(s) 102, 112, 351, 869, 1002, 1083, 1203, 1299
Lsp1109I GCAGC 2 cut(s) 445, 793
LweI GCATC 2 cut(s) 478, 539
MaeI CTAG 3 cut(s) 270, 932, 1372
MaeII ACGT 5 cut(s) 70, 346, 1045, 1120, 1274
MaeIII GTNAC 3 cut(s) 179, 478, 509
MalI GATC 4 cut(s) 37, 284, 966, 1115
MboI GATC 4 cut(s) 35, 282, 964, 1113
MboII GAAGA 6 cut(s) 23, 314, 1072, 1316, 1325, 1328
MfeI CAATTG 4 cut(s) 154, 483, 909, 1206
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 2 cut(s) 770, 814
MluCI AATT 9 cut(s) 64, 154, 483, 909, 1058, 1173, 1206, 1330, 1337
MlyI GAGTC 1 cut(s) 988
MroXI GAANNNNTTC 1 cut(s) 1274
MseI TTAA 6 cut(s) 291, 557, 644, 828, 1055, 1199
MslI CAYNNNNRTG 2 cut(s) 1037, 1088
MspI CCGG 1 cut(s) 1286
MspR9I CCNGG 1 cut(s) 1286
MunI CAATTG 4 cut(s) 154, 483, 909, 1206
Mva1269I GAATGC 1 cut(s) 306
MwoI GCNNNNNNNGC 5 cut(s) 260, 464, 627, 636, 743
NciI CCSGG 1 cut(s) 1286
NdeII GATC 4 cut(s) 35, 282, 964, 1113
NlaIII CATG 8 cut(s) 47, 197, 217, 282, 707, 1131, 1228, 1262
NlaIV GGNNCC 3 cut(s) 37, 622, 1183
NspI RCATGY 2 cut(s) 47, 1262
PagI TCATGA 1 cut(s) 1224
PceI AGGCCT 1 cut(s) 1221
PciI ACATGT 1 cut(s) 43
PciSI GCTCTTC 1 cut(s) 6
PctI GAATGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 1274
PfeI GAWTC 3 cut(s) 857, 928, 1307
PkrI GCNGC 2 cut(s) 460, 783
PleI GAGTC 1 cut(s) 988
PpsI GAGTC 1 cut(s) 988
Ppu21I YACGTR 1 cut(s) 71
PpuMI RGGWCCY 1 cut(s) 1182
PscI ACATGT 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 1182
PspN4I GGNNCC 3 cut(s) 37, 622, 1183
PspPI GGNCC 3 cut(s) 86, 869, 1182
PspPPI RGGWCCY 1 cut(s) 1182
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 4 cut(s) 42, 496, 939, 947
RsaNI GTAC 4 cut(s) 41, 495, 938, 946
RseI CAYNNNNRTG 2 cut(s) 1037, 1088
SapI GCTCTTC 1 cut(s) 6
SaqAI TTAA 6 cut(s) 291, 557, 644, 828, 1055, 1199
SatI GCNGC 2 cut(s) 459, 782
Sau3AI GATC 4 cut(s) 35, 282, 964, 1113
Sau96I GGNCC 3 cut(s) 86, 869, 1182
ScaI AGTACT 1 cut(s) 939
SchI GAGTC 1 cut(s) 988
ScrFI CCNGG 1 cut(s) 1286
SduI GDGCHC 2 cut(s) 770, 814
SfaNI GCATC 2 cut(s) 478, 539
SfcI CTRYAG 2 cut(s) 201, 918
SinI GGWCC 3 cut(s) 86, 869, 1182
SmiMI CAYNNNNRTG 2 cut(s) 1037, 1088
SmlI CTYRAG 1 cut(s) 1265
SmoI CTYRAG 1 cut(s) 1265
SnaBI TACGTA 1 cut(s) 71
Sse9I AATT 9 cut(s) 64, 154, 483, 909, 1058, 1173, 1206, 1330, 1337
SseBI AGGCCT 1 cut(s) 1221
SsiI CCGC 1 cut(s) 94
SspMI CTAG 3 cut(s) 270, 932, 1372
StuI AGGCCT 1 cut(s) 1221
StyD4I CCNGG 1 cut(s) 1284
StyI CCWWGG 2 cut(s) 169, 624
TaaI ACNGT 3 cut(s) 435, 892, 942
TaiI ACGT 5 cut(s) 73, 349, 1048, 1123, 1277
TaqI TCGA 2 cut(s) 51, 926
TasI AATT 9 cut(s) 64, 154, 483, 909, 1058, 1173, 1206, 1330, 1337
TatI WGTACW 3 cut(s) 494, 937, 945
TfiI GAWTC 3 cut(s) 857, 928, 1307
Tru1I TTAA 6 cut(s) 291, 557, 644, 828, 1055, 1199
Tru9I TTAA 6 cut(s) 291, 557, 644, 828, 1055, 1199
TscAI CASTG 4 cut(s) 131, 319, 438, 859
TseI GCWGC 2 cut(s) 458, 781
TspDTI ATGAA 9 cut(s) 230, 267, 346, 599, 692, 755, 790, 889, 1072
TspGWI ACGGA 4 cut(s) 28, 960, 965, 1185
TspRI CASTG 4 cut(s) 131, 319, 438, 859
VpaK11BI GGWCC 3 cut(s) 86, 869, 1182
XapI RAATTY 2 cut(s) 1058, 1330
XceI RCATGY 2 cut(s) 47, 1262
XmnI GAANNNNTTC 1 cut(s) 1274
XspI CTAG 3 cut(s) 270, 932, 1372
ZraI GACGTC 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.