AT5G25050

Transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008245
Physical Location & Seq
Forward (+)
8631461 .. 8634027
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25050.1

Sequence Viewer

Length: 1500 bp
ATGGTAGTAGAAGAGCAAAGCCTTGAAAACGGCGGATCCGTACATGTGGTCAAAGGAGAAAGTAATTTCCGTAATGTGGTGTGTGGTCCTGTACGGTGGCTAAAGATGTTGTCTTCAGAGCTTCATTGGAGCTTTGTGTTTGGTGTAGTCTCACTATATGGAATTAACCAAGGGCTTGGTGGCTCATTAGGTCGTGTAGCTACAGAGTATTATATGAAAGATGTTCAAAAAGTTCAGCCTTCTGAATCTCAAGCTCTCACTGCAATCACTAAGATTCCATGGATCATTAAACCACTTTGGGGCATTCTCACTGATGTTCTTCCCATCTTTGGTTTCCATAGACGTCCTTACTTCATTTTAGCAGGGGTGCTTGGAGTGGTTTCATTGTTGTTTATTTCACTGCATAGTAATCTACACCTTTACTTGGCACTGTTTTGGATGACAATATCAAGTGCTGCAATGGCGATTGCTGATGTAACCATTGATGCTTGTACTGCTTACAATAGTATCAAACATCCTTCTCTTGCATCGGATATGCAGAGTTTATGCAGTTTAAGCTCTTCCATTGGAGCACTTCTTGGTTTCTTTATGAGTGGCATCTTAGTTCATCTTGTTGGCTCTAAGGGTGTCTTTGGATTGCTGACATTCCCATTCGCGTTAGTATCGGTAGTCGGGATCGTGTTTAGTGAGCCCCATGTTCCTGGTTTCTCTTACAAACAGGTGAACCAGAAATTTACCGATGCAGGGAAAGCGATGTGGAGGACAATGAAGTGTTCAGATGTGTGGCGGCCGAGTTTGTATATGTACATTTCCCTTACTCTTGGTTTGAACATACATGAAGGTCTCTTCTATTGGTTCACAGATTCAAAGGATGGTCCTTTGTTTGCTCAGGAAACAGTTGGTTTCATTCTCTCCATTGGTTCAATCGGATCGATTCTAGCAGCAACGTTATACCAACTAGTCCTAAAGGATCATCCGTTCCGCGGACTTTGTTTGTGGACTCAACTTCTCTTTGCTCTATCGGGAATGCTTGACCTCATTCTTGTGTTCCGTCTCAACTTAAAATTTGGTTTACCGGATTATCTCTTCATAGTAGTGGACGAGATAGTTTCTCAGATGATCGGACGCTTGAAATGGATGCCGCTGCTTGTCCTCACTTCAAAGCTTTGTCCCCACGGAATTGAAGGGACGTTCTTTGCATTGCTAATGTCAATTGACAATGCAGGCCTCATGACCTCATCTTGGCTTGGAGGGATAATGTTGCATGTCCTCAAGGTGACTCGGACTGAGTTTGGTAACCTGTGGCTTGCGGTTTTGGTCAGGAATGTCATGAGACTTTTGCCGCTTTGTTTTCTGTTTCTTGTGCCTAAAGGAGATCAAAACACGTTCAAGCTCCCGGATGAGATAATGGGCGAAGATTCGGACGAAGAGAAAGATGAAAAAGAAGGAACTCGGAATTTGGAATTGGCTTCTCTTGTTCACTCTGTTGATAGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

499

Amino Acids

55.46

Weight (kDa)

6.67

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BT1 PF03092 57 - 459 2.6e-115 BT1 family
MFS_1 PF07690 78 - 328 1.1e-06 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 346
AccII CGCG 2 cut(s) 656, 984
AciI CCGC 7 cut(s) 33, 787, 982, 984, 1142, 1310, 1343
AclI AACGTT 1 cut(s) 947
AclWI GGATC 6 cut(s) 30, 43, 290, 683, 937, 978
AcoI YGGCCR 1 cut(s) 788
AcsI RAATTY 3 cut(s) 731, 1064, 1456
AcuI CTGAAG 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 343
AfaI GTAC 4 cut(s) 42, 93, 493, 806
AfiI CCNNNNNNNGG 7 cut(s) 76, 299, 329, 424, 744, 983, 1242
AflIII ACRYGT 2 cut(s) 43, 1383
AgsI TTSAA 9 cut(s) 26, 227, 829, 867, 924, 1132, 1161, 1184, 1390
AhlI ACTAGT 1 cut(s) 958
AjnI CCWGG 1 cut(s) 700
AjuI GAANNNNNNNTTGG 2 cut(s) 1448, 1480
AloI GAACNNNNNNTCC 2 cut(s) 962, 994
AluBI AGCT 7 cut(s) 121, 132, 200, 254, 558, 1165, 1393
AluI AGCT 7 cut(s) 121, 132, 200, 254, 558, 1165, 1393
Alw21I GWGCWC 1 cut(s) 574
Alw26I GTCTC 4 cut(s) 154, 848, 1058, 1327
AlwI GGATC 6 cut(s) 30, 43, 290, 683, 937, 978
AoxI GGCC 2 cut(s) 788, 1225
ApeKI GCWGC 3 cut(s) 455, 941, 1144
ApoI RAATTY 3 cut(s) 731, 1064, 1456
AspS9I GGNCC 2 cut(s) 86, 875
AsuC2I CCSGG 1 cut(s) 1397
AsuHPI GGTGA 2 cut(s) 733, 1288
AvaII GGWCC 2 cut(s) 86, 875
BaeI ACNNNNGTAYC 2 cut(s) 490, 523
BamHI GGATCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 693
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 574
BbvI GCAGC 3 cut(s) 442, 953, 1131
BccI CCATC 2 cut(s) 332, 866
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 702
BcnI CCSGG 1 cut(s) 1397
BcoDI GTCTC 4 cut(s) 154, 848, 1058, 1327
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 2 cut(s) 938, 959
BfmI CTRYAG 1 cut(s) 201
BisI GCNGC 6 cut(s) 456, 788, 942, 1142, 1145, 1343
BlsI GCNGC 6 cut(s) 457, 789, 943, 1143, 1146, 1344
Bme1390I CCNGG 2 cut(s) 702, 1397
Bme18I GGWCC 2 cut(s) 86, 875
BmgT120I GGNCC 2 cut(s) 86, 875
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 2 cut(s) 702, 1397
BmsI GCATC 5 cut(s) 475, 536, 606, 730, 1128
BpiI GAAGAC 1 cut(s) 105
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 234, 1256
BpuMI CCSGG 1 cut(s) 1397
Bsa29I ATCGAT 1 cut(s) 932
BsaHI GRCGYC 1 cut(s) 343
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 4 cut(s) 169, 278, 982, 1174
BsaWI WCCGGW 1 cut(s) 1075
Bsc4I CCNNNNNNNGG 7 cut(s) 76, 299, 329, 424, 744, 983, 1242
Bse3DI GCAATG 2 cut(s) 465, 1199
BseBI CCWGG 1 cut(s) 702
BseCI ATCGAT 1 cut(s) 932
BseDI CCNNGG 4 cut(s) 169, 278, 982, 1174
BseGI GGATG 6 cut(s) 444, 514, 877, 973, 1143, 1405
BseLI CCNNNNNNNGG 7 cut(s) 76, 299, 329, 424, 744, 983, 1242
BseMI GCAATG 2 cut(s) 465, 1199
BseMII CTCAG 3 cut(s) 902, 1127, 1278
BseX3I CGGCCG 1 cut(s) 788
BseXI GCAGC 3 cut(s) 442, 953, 1131
Bsh1236I CGCG 2 cut(s) 656, 984
Bsh1285I CGRYCG 1 cut(s) 791
BshFI GGCC 2 cut(s) 790, 1227
BshVI ATCGAT 1 cut(s) 932
BsiEI CGRYCG 1 cut(s) 791
BsiHKAI GWGCWC 1 cut(s) 574
BsiSI CCGG 2 cut(s) 1076, 1397
BslFI GGGAC 2 cut(s) 1155, 1201
BslI CCNNNNNNNGG 7 cut(s) 76, 299, 329, 424, 744, 983, 1242
BsmAI GTCTC 4 cut(s) 154, 848, 1058, 1327
BsmBI CGTCTC 1 cut(s) 1058
BsmFI GGGAC 2 cut(s) 1155, 1201
BsmI GAATGC 2 cut(s) 303, 1032
BsnI GGCC 2 cut(s) 790, 1227
Bso31I GGTCTC 1 cut(s) 848
Bsp1286I GDGCHC 2 cut(s) 574, 693
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
Bsp19I CCATGG 1 cut(s) 278
BspACI CCGC 7 cut(s) 33, 787, 982, 984, 1142, 1310, 1343
BspANI GGCC 2 cut(s) 790, 1227
BspCNI CTCAG 3 cut(s) 901, 1126, 1279
BspDI ATCGAT 1 cut(s) 932
BspFNI CGCG 2 cut(s) 656, 984
BspHI TCATGA 2 cut(s) 1230, 1329
BspLI GGNNCC 1 cut(s) 37
BspPI GGATC 6 cut(s) 30, 43, 290, 683, 937, 978
BspQI GCTCTTC 2 cut(s) 6, 565
BspTNI GGTCTC 1 cut(s) 848
BsrDI GCAATG 2 cut(s) 465, 1199
BsrGI TGTACA 1 cut(s) 804
BssECI CCNNGG 4 cut(s) 169, 278, 982, 1174
BssMI GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 169, 278
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 3 cut(s) 96, 432, 898
Bst6I CTCTTC 5 cut(s) 6, 565, 851, 1091, 1422
BstACI GRCGYC 1 cut(s) 343
BstAUI TGTACA 1 cut(s) 804
BstC8I GCNNGC 2 cut(s) 1225, 1308
BstDEI CTNAG 6 cut(s) 270, 601, 621, 888, 1113, 1287
BstDSI CCRYGG 3 cut(s) 278, 982, 1174
BstEII GGTNACC 1 cut(s) 1295
BstF5I GGATG 6 cut(s) 444, 514, 877, 973, 1143, 1405
BstFNI CGCG 2 cut(s) 656, 984
BstKTI GATC 7 cut(s) 38, 285, 678, 932, 973, 1122, 1378
BstMAI GTCTC 4 cut(s) 154, 848, 1058, 1327
BstMBI GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
BstMCI CGRYCG 1 cut(s) 791
BstMWI GCNNNNNNNGC 5 cut(s) 260, 461, 494, 555, 749
BstNI CCWGG 1 cut(s) 702
BstNSI RCATGY 2 cut(s) 47, 1268
BstPI GGTNACC 1 cut(s) 1295
BstSCI CCNGG 2 cut(s) 700, 1395
BstSFI CTRYAG 1 cut(s) 201
BstUI CGCG 2 cut(s) 656, 984
BstV1I GCAGC 3 cut(s) 442, 953, 1131
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 35
BstXI CCANNNNNNTGG 2 cut(s) 176, 701
BstYI RGATCY 1 cut(s) 35
BstZI CGGCCG 1 cut(s) 788
Bsu15I ATCGAT 1 cut(s) 932
BsuRI GGCC 2 cut(s) 790, 1227
BsuTUI ATCGAT 1 cut(s) 932
BtgI CCRYGG 3 cut(s) 278, 982, 1174
BtgZI GCGATG 1 cut(s) 767
BtsCI GGATG 6 cut(s) 444, 514, 877, 973, 1143, 1405
BtsI GCAGTG 2 cut(s) 258, 398
BtsIMutI CAGTG 4 cut(s) 258, 309, 398, 428
Cac8I GCNNGC 2 cut(s) 1225, 1308
CciI TCATGA 2 cut(s) 1230, 1329
Cfr13I GGNCC 2 cut(s) 86, 875
Cfr42I CCGCGG 1 cut(s) 985
ClaI ATCGAT 1 cut(s) 932
CseI GACGC 1 cut(s) 1134
Csp6I GTAC 4 cut(s) 41, 92, 492, 805
CviAII CATG 7 cut(s) 44, 279, 695, 836, 1231, 1265, 1330
CviQI GTAC 4 cut(s) 41, 92, 492, 805
DdeI CTNAG 6 cut(s) 270, 601, 621, 888, 1113, 1287
DpnI GATC 7 cut(s) 37, 284, 677, 931, 972, 1121, 1377
DpnII GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
EaeI YGGCCR 1 cut(s) 788
EagI CGGCCG 1 cut(s) 788
Eam1104I CTCTTC 5 cut(s) 6, 565, 851, 1091, 1422
EarI CTCTTC 5 cut(s) 6, 565, 851, 1091, 1422
EciI GGCGGA 1 cut(s) 48
EclXI CGGCCG 1 cut(s) 788
Eco130I CCWWGG 2 cut(s) 169, 278
Eco147I AGGCCT 1 cut(s) 1227
Eco24I GRGCYC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 2 cut(s) 86, 875
Eco52I CGGCCG 1 cut(s) 788
Eco57I CTGAAG 1 cut(s) 99
Eco91I GGTNACC 1 cut(s) 1295
EcoO65I GGTNACC 1 cut(s) 1295
EcoRII CCWGG 1 cut(s) 700
EcoT14I CCWWGG 2 cut(s) 169, 278
EcoT38I GRGCYC 1 cut(s) 693
ErhI CCWWGG 2 cut(s) 169, 278
Esp3I CGTCTC 1 cut(s) 1058
FaeI CATG 7 cut(s) 47, 282, 698, 839, 1234, 1268, 1333
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 2 cut(s) 1155, 1201
FatI CATG 7 cut(s) 43, 278, 694, 835, 1230, 1264, 1329
Fnu4HI GCNGC 6 cut(s) 456, 788, 942, 1142, 1145, 1343
FokI GGATG 6 cut(s) 451, 501, 884, 960, 1150, 1412
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 6 cut(s) 456, 788, 942, 1142, 1145, 1343
FspBI CTAG 2 cut(s) 938, 959
GluI GCNGC 6 cut(s) 456, 788, 942, 1142, 1145, 1343
HaeIII GGCC 2 cut(s) 790, 1227
HapII CCGG 2 cut(s) 1076, 1397
HgaI GACGC 1 cut(s) 1134
Hin1I GRCGYC 1 cut(s) 343
Hin1II CATG 7 cut(s) 47, 282, 698, 839, 1234, 1268, 1333
HindIII AAGCTT 1 cut(s) 1163
HinfI GANTC 7 cut(s) 245, 274, 863, 934, 1000, 1279, 1418
HpaII CCGG 2 cut(s) 1076, 1397
HphI GGTGA 2 cut(s) 733, 1288
Hpy166II GTNNAC 6 cut(s) 724, 858, 999, 1073, 1099, 1480
Hpy188III TCNNGA 6 cut(s) 673, 890, 1023, 1231, 1321, 1330
Hpy8I GTNNAC 6 cut(s) 724, 858, 999, 1073, 1099, 1480
HpyAV CCTTC 5 cut(s) 249, 528, 833, 1178, 1439
HpyCH4III ACNGT 3 cut(s) 96, 432, 898
HpyCH4IV ACGT 4 cut(s) 343, 947, 1190, 1385
HpyF10VI GCNNNNNNNGC 5 cut(s) 260, 461, 494, 555, 749
HpyF3I CTNAG 6 cut(s) 270, 601, 621, 888, 1113, 1287
HpySE526I ACGT 4 cut(s) 343, 947, 1190, 1385
Hsp92I GRCGYC 1 cut(s) 343
Hsp92II CATG 7 cut(s) 47, 282, 698, 839, 1234, 1268, 1333
KspI CCGCGG 1 cut(s) 985
Kzo9I GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
LguI GCTCTTC 2 cut(s) 6, 565
LmnI GCTCC 3 cut(s) 129, 569, 1398
Lsp1109I GCAGC 3 cut(s) 442, 953, 1131
LweI GCATC 5 cut(s) 475, 536, 606, 730, 1128
MaeI CTAG 2 cut(s) 938, 959
MaeII ACGT 4 cut(s) 343, 947, 1190, 1385
MaeIII GTNAC 3 cut(s) 475, 1276, 1295
MalI GATC 7 cut(s) 37, 284, 677, 931, 972, 1121, 1377
MboI GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
MboII GAAGA 8 cut(s) 23, 105, 311, 552, 838, 1078, 1427, 1439
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 1 cut(s) 35
MhlI GDGCHC 2 cut(s) 574, 693
MluCI AATT 8 cut(s) 64, 162, 731, 1064, 1179, 1212, 1456, 1463
MlyI GAGTC 2 cut(s) 994, 1273
MnlI CCTC 7 cut(s) 753, 1046, 1163, 1238, 1244, 1246, 1280
MseI TTAA 4 cut(s) 165, 288, 554, 1061
MslI CAYNNNNRTG 2 cut(s) 1043, 1094
MspA1I CMGCKG 2 cut(s) 984, 1144
MspI CCGG 2 cut(s) 1076, 1397
MspR9I CCNGG 2 cut(s) 702, 1397
MunI CAATTG 1 cut(s) 1212
Mva1269I GAATGC 2 cut(s) 303, 1032
MvaI CCWGG 1 cut(s) 702
MvnI CGCG 2 cut(s) 656, 984
MwoI GCNNNNNNNGC 5 cut(s) 260, 461, 494, 555, 749
NciI CCSGG 1 cut(s) 1397
NcoI CCATGG 1 cut(s) 278
NdeII GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
NlaIII CATG 7 cut(s) 47, 282, 698, 839, 1234, 1268, 1333
NlaIV GGNNCC 1 cut(s) 37
NmeAIII GCCGAG 1 cut(s) 816
NmuCI GTSAC 1 cut(s) 1276
NspI RCATGY 2 cut(s) 47, 1268
PagI TCATGA 2 cut(s) 1230, 1329
PceI AGGCCT 1 cut(s) 1227
PciI ACATGT 1 cut(s) 43
PciSI GCTCTTC 2 cut(s) 6, 565
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 2 cut(s) 303, 1032
PfeI GAWTC 5 cut(s) 245, 274, 863, 934, 1418
PfoI TCCNGGA 1 cut(s) 1395
PkrI GCNGC 6 cut(s) 457, 789, 943, 1143, 1146, 1344
PleI GAGTC 2 cut(s) 994, 1273
PpsI GAGTC 2 cut(s) 994, 1273
PscI ACATGT 1 cut(s) 43
Psp1406I AACGTT 1 cut(s) 947
Psp6I CCWGG 1 cut(s) 700
PspEI GGTNACC 1 cut(s) 1295
PspGI CCWGG 1 cut(s) 700
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 2 cut(s) 86, 875
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 4 cut(s) 42, 93, 493, 806
RsaNI GTAC 4 cut(s) 41, 92, 492, 805
RseI CAYNNNNRTG 2 cut(s) 1043, 1094
SacII CCGCGG 1 cut(s) 985
SapI GCTCTTC 2 cut(s) 6, 565
SaqAI TTAA 4 cut(s) 165, 288, 554, 1061
SatI GCNGC 6 cut(s) 456, 788, 942, 1142, 1145, 1343
Sau3AI GATC 7 cut(s) 35, 282, 675, 929, 970, 1119, 1375
Sau96I GGNCC 2 cut(s) 86, 875
SchI GAGTC 2 cut(s) 994, 1273
ScrFI CCNGG 2 cut(s) 702, 1397
SduI GDGCHC 2 cut(s) 574, 693
SfaNI GCATC 5 cut(s) 475, 536, 606, 730, 1128
SfcI CTRYAG 1 cut(s) 201
Sfr303I CCGCGG 1 cut(s) 985
SgrBI CCGCGG 1 cut(s) 985
SinI GGWCC 2 cut(s) 86, 875
SmiMI CAYNNNNRTG 2 cut(s) 1043, 1094
SmlI CTYRAG 2 cut(s) 249, 1271
SmoI CTYRAG 2 cut(s) 249, 1271
SpeI ACTAGT 1 cut(s) 958
Sse9I AATT 8 cut(s) 64, 162, 731, 1064, 1179, 1212, 1456, 1463
SseBI AGGCCT 1 cut(s) 1227
SsiI CCGC 7 cut(s) 33, 787, 982, 984, 1142, 1310, 1343
SspMI CTAG 2 cut(s) 938, 959
StuI AGGCCT 1 cut(s) 1227
StyD4I CCNGG 2 cut(s) 700, 1395
StyI CCWWGG 2 cut(s) 169, 278
TaaI ACNGT 3 cut(s) 96, 432, 898
TaiI ACGT 4 cut(s) 346, 950, 1193, 1388
TaqI TCGA 1 cut(s) 932
TasI AATT 8 cut(s) 64, 162, 731, 1064, 1179, 1212, 1456, 1463
TatI WGTACW 2 cut(s) 491, 804
TauI GCSGC 3 cut(s) 790, 1144, 1345
TfiI GAWTC 5 cut(s) 245, 274, 863, 934, 1418
Tru1I TTAA 4 cut(s) 165, 288, 554, 1061
Tru9I TTAA 4 cut(s) 165, 288, 554, 1061
TscAI CASTG 4 cut(s) 265, 316, 405, 435
TseFI GTSAC 1 cut(s) 1276
TseI GCWGC 3 cut(s) 455, 941, 1144
Tsp45I GTSAC 1 cut(s) 1276
TspGWI ACGGA 5 cut(s) 28, 59, 966, 1040, 1191
TspRI CASTG 4 cut(s) 265, 316, 405, 435
VpaK11BI GGWCC 2 cut(s) 86, 875
XapI RAATTY 3 cut(s) 731, 1064, 1456
XceI RCATGY 2 cut(s) 47, 1268
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XspI CTAG 2 cut(s) 938, 959
ZraI GACGTC 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.