RLG00000011448

Ubiquitinyl hydrolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012946
Physical Location & Seq
Reverse (-)
9511503 .. 9513977
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011448

Sequence Viewer

Length: 1659 bp
ATGTCGACAAGAAAACCTTCATACACAAACCCAAGACCCTGCAAGCACCTTTCAGCTTACAAGCTCAAACATGGCCTGAGTGGGTATAAATCACTCCAAAAGTGCCTCAAGACGACCCCAAATGGAAGGACAAGTGTTGAGAATCCGGGCACAAAGATATCCAGATGCAGTTCCTGTAATGGGTATCAAGGTAGACTCTATTTTTGTCTGGTTTGCTCTTCAATCTCGTGCTCAAGTCACACCCTTTTGCACACCCAGTCTGAGATTGGCCATGATTTGGCGGTGGACATTGAAAGGGCTGAGCTTTATTGCTGTGCCTGCTCTGATCAGGTCTACGATCCTGATTTTGACAAGACTGTCATGTCTAAACACATGTTGGACTTGAGTTATAGCACAAACAGAAACAGTGCAGATAGTATAGGGGAGAGACTCTGTGAGAGGAAGAGGCTGGGTTCTGGGGTAGAATTGGCAGATGTGAACAAGTCAGGACAGCTGGCTTTGATGAGAGATCCTAGGGCTAAATCATGTTATCCATTAGGTTTGAGAGGATTGAATAATTTGGGCAATACTTGTTTCATGAATTCTGTGTTGCAAGCACTGTTGCATGCTCCTCCAATGAGAAATTACTTCTTGAGTGATCGGCATAACCGCAGGACGTGCAGGAAGAGGTCGGCCAACCGGCTCTGTCTCCCTTGTGAGCTTGATGGTATCTTCTCCGCTGTCTATTCGGGTGGTCGTGATCGCACACCTTATAGTCCGGCTCAATTTCTTTACAGTTGGTGGCAGCATTCAGAAAATCTGGCAAGTTATGAGCAGCAGGATGCTCATGAGTTTTTCATTTCAGTGTTAGATGGGATCCATGAGAGAGAAAGCAAAGCAAGGAACCAAACTAGAGATAGTGGAGATTGCCAGTGTATTACTCATAGGGTCTTCTCTGGACAGTTGAGATCAGATGTCACTTGTATGACTTGTGGATTCACTTCAACAACTTATGACCCTTGTTTGGACATATCGCTCGACTTGGACACAAACCATTGCTCTAATAAGCCTGTCAAACAAAATGACAACAGCAGTACATCTACTCTTCTTGGTTGTTTAGACTTGTTCACTAAACCAGAGAAGTTGGGAACAGACCAGAAGCTGTTTTGTCAAAACTGTCAAGAACCTCGAGACTCATCGAAGCAACTGTCTATTAGAAAGCTCCCTTTGGTGTTGTGTTTGCATATAAAACGATTCGAGCACTCCCTGGTCAGAAAGATGATGAGGAAAATCGACAGCTATATGCATTTTCCTTTCTCCTTAGACATGACTCCATATCTGTCCTCTTCAATTATCAGAAACAGATTTGGGAATAGAATCTTTGCCTTTGAGGGTGATGATTCCGATAATTCTACAGAATTTGAGATTTTTGCAGTTATTACTCATTCTGGGACGCTAGACTCTGGCCATTATTTAACTTATTTACGCATAAAAGAGCAATGGTATAAGTGTGACAATGCTTGGATTAGTGAGGTTGATGAGAGGGTTGTAAGAGCTTCACAGTGCTACATGATGTTTTATGTGCACAAGACGCTTTACTATAAAGCAAAAGAAGATTTGAGTTGCTTGCCAGTTTCGGCACGGAAGGAGGCTTTTCATAGGGTTGCCGGTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

553

Amino Acids

62.89

Weight (kDa)

8.61

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UBP PF02148 56 - 115 8e-10 Zn-finger in ubiquitin-hydrolases and other protein
UCH PF00443 182 - 520 1.1e-59 Ubiquitin carboxyl-terminal hydrolase
UCH_1 PF13423 183 - 495 5.7e-19 Ubiquitin carboxyl-terminal hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10790 AT5G10790
fragaria_vesca FvH4_2g41240
malus_domestica MD08G1052900.v1.1 MD15G1042700.v1.1
prunus_persica Prupe.1G396000_v2.0.a1
pyrus_communis pycom08g04410 pycom15g03980
rosa_chinensis RchiOBHm_Chr6g0299261
rosa_laevigata RLG00000011448
rosa_multiflora Rmu_sc0001434.1_g000005
rosa_roxburghii Rroxscaffold_7G00168850
rosa_rugosa Rorug06G0286700
rosa_samantha Rh6AG398000 Rh6BG406500 Rh6CG412600 Rh6DG399300
rosa_wichuraiana Rw6G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 356, 361
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 3 cut(s) 5, 193, 333
AciI CCGC 3 cut(s) 281, 649, 717
AclWI GGATC 4 cut(s) 332, 503, 850, 863
AcoI YGGCCR 3 cut(s) 268, 672, 1444
AcsI RAATTY 2 cut(s) 580, 1397
AfaI GTAC 1 cut(s) 1075
AfiI CCNNNNNNNGG 3 cut(s) 180, 277, 1003
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 5 cut(s) 222, 293, 553, 984, 1329
AjiI CACGTC 1 cut(s) 657
AjnI CCWGG 1 cut(s) 1245
AluBI AGCT 9 cut(s) 56, 64, 304, 493, 700, 1141, 1201, 1278, 1535
AluI AGCT 9 cut(s) 56, 64, 304, 493, 700, 1141, 1201, 1278, 1535
Alw21I GWGCWC 3 cut(s) 233, 1242, 1566
Alw26I GTCTC 3 cut(s) 421, 692, 1164
Alw44I GTGCAC 1 cut(s) 1562
AlwI GGATC 4 cut(s) 332, 503, 850, 863
AlwNI CAGNNNCTG 2 cut(s) 174, 1141
Ama87I CYCGRG 1 cut(s) 1167
AoxI GGCC 4 cut(s) 73, 268, 672, 1444
ApaLI GTGCAC 1 cut(s) 1562
ApeKI GCWGC 3 cut(s) 784, 814, 1653
ApoI RAATTY 2 cut(s) 580, 1397
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 1172, 1204
Asp700I GAANNNNTTC 1 cut(s) 16
AspA2I CCTAGG 1 cut(s) 512
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 1385
AvaI CYCGRG 1 cut(s) 1167
AvrII CCTAGG 1 cut(s) 512
BaeGI GKGCMC 2 cut(s) 152, 1566
BalI TGGCCA 2 cut(s) 270, 1446
BamHI GGATCC 1 cut(s) 855
BauI CACGAG 1 cut(s) 226
BbsI GAAGAC 1 cut(s) 922
Bbv12I GWGCWC 3 cut(s) 233, 1242, 1566
BbvI GCAGC 3 cut(s) 796, 826, 1640
BccI CCATC 2 cut(s) 698, 845
BciT130I CCWGG 1 cut(s) 1247
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 421, 692, 1164
BfaI CTAG 3 cut(s) 513, 891, 1436
BfmI CTRYAG 1 cut(s) 1392
BisI GCNGC 3 cut(s) 785, 815, 1654
BlnI CCTAGG 1 cut(s) 512
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 3 cut(s) 786, 816, 1655
Bme1390I CCNGG 2 cut(s) 147, 1247
BmeT110I CYCGRG 1 cut(s) 1167
BmgBI CACGTC 1 cut(s) 657
BmiI GGNNCC 2 cut(s) 857, 884
BmrFI CCNGG 2 cut(s) 147, 1247
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 155, 811
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 922
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 4 cut(s) 92, 217, 403, 652
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 512, 1245
Bsc4I CCNNNNNNNGG 3 cut(s) 180, 277, 1003
Bse118I RCCGGY 2 cut(s) 678, 1646
Bse1I ACTGG 3 cut(s) 256, 910, 1610
Bse3DI GCAATG 2 cut(s) 1033, 1484
BseBI CCWGG 1 cut(s) 1247
BseDI CCNNGG 2 cut(s) 512, 1245
BseGI GGATG 1 cut(s) 826
BseLI CCNNNNNNNGG 3 cut(s) 180, 277, 1003
BseMI GCAATG 2 cut(s) 1033, 1484
BseMII CTCAG 3 cut(s) 68, 252, 291
BseNI ACTGG 3 cut(s) 256, 910, 1610
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 2 cut(s) 152, 1566
BseXI GCAGC 3 cut(s) 796, 826, 1640
BseYI CCCAGC 1 cut(s) 448
BsgI GTGCAG 2 cut(s) 429, 679
BshFI GGCC 4 cut(s) 75, 270, 674, 1446
BsiHKAI GWGCWC 3 cut(s) 233, 1242, 1566
BsiHKCI CYCGRG 1 cut(s) 1167
BsiSI CCGG 4 cut(s) 146, 679, 758, 1647
BslFI GGGAC 1 cut(s) 1444
BslI CCNNNNNNNGG 3 cut(s) 180, 277, 1003
BsmAI GTCTC 3 cut(s) 421, 692, 1164
BsmFI GGGAC 1 cut(s) 1444
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 4 cut(s) 75, 270, 674, 1446
BsoBI CYCGRG 1 cut(s) 1167
Bsp1286I GDGCHC 4 cut(s) 152, 233, 1242, 1566
Bsp143I GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 3 cut(s) 281, 649, 717
BspANI GGCC 4 cut(s) 75, 270, 674, 1446
BspCNI CTCAG 3 cut(s) 69, 253, 292
BspHI TCATGA 2 cut(s) 576, 826
BspLI GGNNCC 2 cut(s) 857, 884
BspPI GGATC 4 cut(s) 332, 503, 850, 863
BspQI GCTCTTC 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 1033, 1484
BsrFI RCCGGY 2 cut(s) 678, 1646
BsrI ACTGG 3 cut(s) 256, 910, 1610
BssAI RCCGGY 2 cut(s) 678, 1646
BssECI CCNNGG 2 cut(s) 512, 1245
BssMI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
BssSI CACGAG 1 cut(s) 226
BssT1I CCWWGG 1 cut(s) 512
Bst2BI CACGAG 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 1247
Bst4CI ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
Bst6I CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
BstAPI GCANNNNNTGC 1 cut(s) 657
BstC8I GCNNGC 6 cut(s) 44, 319, 495, 594, 606, 1607
BstDEI CTNAG 4 cut(s) 77, 261, 300, 1300
BstF5I GGATG 1 cut(s) 826
BstKTI GATC 7 cut(s) 328, 340, 511, 640, 742, 858, 950
BstMAI GTCTC 3 cut(s) 421, 692, 1164
BstMBI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
BstMWI GCNNNNNNNGC 3 cut(s) 318, 657, 1570
BstNI CCWGG 1 cut(s) 1247
BstNSI RCATGY 2 cut(s) 376, 608
BstSCI CCNGG 2 cut(s) 145, 1245
BstSFI CTRYAG 1 cut(s) 1392
BstSLI GKGCMC 2 cut(s) 152, 1566
BstV1I GCAGC 3 cut(s) 796, 826, 1640
BstV2I GAAGAC 1 cut(s) 922
BstX2I RGATCY 2 cut(s) 508, 855
BstYI RGATCY 2 cut(s) 508, 855
BsuRI GGCC 4 cut(s) 75, 270, 674, 1446
BtrI CACGTC 1 cut(s) 657
BtsCI GGATG 1 cut(s) 826
BtsIMutI CAGTG 5 cut(s) 412, 596, 849, 917, 1547
Cac8I GCNNGC 6 cut(s) 44, 319, 495, 594, 606, 1607
CaiI CAGNNNCTG 2 cut(s) 174, 1141
CciI TCATGA 2 cut(s) 576, 826
Cfr10I RCCGGY 2 cut(s) 678, 1646
CseI GACGC 2 cut(s) 1441, 1579
Csp6I GTAC 1 cut(s) 1074
CviQI GTAC 1 cut(s) 1074
DdeI CTNAG 4 cut(s) 77, 261, 300, 1300
DpnI GATC 7 cut(s) 327, 339, 510, 639, 741, 857, 949
DpnII GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
DrdI GACNNNNNNGTC 2 cut(s) 356, 361
DseDI GACNNNNNNGTC 2 cut(s) 356, 361
EaeI YGGCCR 3 cut(s) 268, 672, 1444
Eam1104I CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
EarI CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
Eco130I CCWWGG 1 cut(s) 512
Eco32I GATATC 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 1167
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 1 cut(s) 1245
EcoRV GATATC 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 512
EcoT22I ATGCAT 1 cut(s) 1287
ErhI CCWWGG 1 cut(s) 512
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 1 cut(s) 1444
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 3 cut(s) 5, 193, 333
Fnu4HI GCNGC 3 cut(s) 785, 815, 1654
FokI GGATG 1 cut(s) 833
Fsp4HI GCNGC 3 cut(s) 785, 815, 1654
FspBI CTAG 3 cut(s) 513, 891, 1436
GluI GCNGC 3 cut(s) 785, 815, 1654
GsaI CCCAGC 1 cut(s) 452
HaeIII GGCC 4 cut(s) 75, 270, 674, 1446
HapII CCGG 4 cut(s) 146, 679, 758, 1647
HgaI GACGC 2 cut(s) 1441, 1579
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 4 cut(s) 146, 679, 758, 1647
HphI GGTGA 1 cut(s) 1385
Hpy166II GTNNAC 7 cut(s) 6, 194, 286, 334, 478, 1107, 1564
Hpy188I TCNGA 7 cut(s) 262, 325, 793, 952, 1253, 1337, 1384
Hpy8I GTNNAC 7 cut(s) 6, 194, 286, 334, 478, 1107, 1564
HpyAV CCTTC 3 cut(s) 27, 120, 1618
HpyCH4III ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
HpyCH4IV ACGT 1 cut(s) 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 318, 657, 1570
HpyF3I CTNAG 4 cut(s) 77, 261, 300, 1300
HpySE526I ACGT 1 cut(s) 656
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 613, 1206
Lsp1109I GCAGC 3 cut(s) 796, 826, 1640
LweI GCATC 2 cut(s) 155, 811
MaeI CTAG 3 cut(s) 513, 891, 1436
MaeII ACGT 1 cut(s) 656
MaeIII GTNAC 3 cut(s) 236, 955, 1490
MalI GATC 7 cut(s) 327, 339, 510, 639, 741, 857, 949
MboI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
MboII GAAGA 8 cut(s) 210, 454, 676, 703, 922, 1076, 1317, 1604
MflI RGATCY 2 cut(s) 508, 855
MhlI GDGCHC 4 cut(s) 152, 233, 1242, 1566
MlsI TGGCCA 2 cut(s) 270, 1446
MluCI AATT 8 cut(s) 464, 556, 580, 622, 764, 1329, 1387, 1397
MluNI TGGCCA 2 cut(s) 270, 1446
MlyI GAGTC 5 cut(s) 189, 423, 1166, 1303, 1433
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 2 cut(s) 270, 1446
Mph1103I ATGCAT 1 cut(s) 1287
MroXI GAANNNNTTC 1 cut(s) 16
MscI TGGCCA 2 cut(s) 270, 1446
MseI TTAA 1 cut(s) 1454
MslI CAYNNNNRTG 2 cut(s) 842, 962
Msp20I TGGCCA 2 cut(s) 270, 1446
MspA1I CMGCKG 2 cut(s) 493, 719
MspI CCGG 4 cut(s) 146, 679, 758, 1647
MspR9I CCNGG 2 cut(s) 147, 1247
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 1 cut(s) 1247
MwoI GCNNNNNNNGC 3 cut(s) 318, 657, 1570
NciI CCSGG 1 cut(s) 147
NdeII GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
NlaIV GGNNCC 2 cut(s) 857, 884
NmuCI GTSAC 3 cut(s) 236, 955, 1490
NsiI ATGCAT 1 cut(s) 1287
NspI RCATGY 2 cut(s) 376, 608
PaeI GCATGC 1 cut(s) 608
PaeR7I CTCGAG 1 cut(s) 1167
PagI TCATGA 2 cut(s) 576, 826
PciI ACATGT 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 223
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 5 cut(s) 142, 975, 1233, 1356, 1379
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 3 cut(s) 786, 816, 1655
PleI GAGTC 5 cut(s) 189, 423, 1166, 1303, 1433
PpsI GAGTC 5 cut(s) 189, 423, 1166, 1303, 1433
PscI ACATGT 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 1245
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 1 cut(s) 1245
PspN4I GGNNCC 2 cut(s) 857, 884
PstNI CAGNNNCTG 2 cut(s) 174, 1141
PsuI RGATCY 2 cut(s) 508, 855
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 1 cut(s) 1075
RsaNI GTAC 1 cut(s) 1074
RseI CAYNNNNRTG 2 cut(s) 842, 962
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 1454
SatI GCNGC 3 cut(s) 785, 815, 1654
Sau3AI GATC 7 cut(s) 325, 337, 508, 637, 739, 855, 947
SchI GAGTC 5 cut(s) 189, 423, 1166, 1303, 1433
ScrFI CCNGG 2 cut(s) 147, 1247
SduI GDGCHC 4 cut(s) 152, 233, 1242, 1566
SfaNI GCATC 2 cut(s) 155, 811
SfcI CTRYAG 1 cut(s) 1392
Sfr274I CTCGAG 1 cut(s) 1167
SlaI CTCGAG 1 cut(s) 1167
SmiMI CAYNNNNRTG 2 cut(s) 842, 962
SmlI CTYRAG 5 cut(s) 107, 232, 382, 631, 1167
SmoI CTYRAG 5 cut(s) 107, 232, 382, 631, 1167
SphI GCATGC 1 cut(s) 608
Sse9I AATT 8 cut(s) 464, 556, 580, 622, 764, 1329, 1387, 1397
SsiI CCGC 3 cut(s) 281, 649, 717
SspMI CTAG 3 cut(s) 513, 891, 1436
StyD4I CCNGG 2 cut(s) 145, 1245
StyI CCWWGG 1 cut(s) 512
TaaI ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
TaiI ACGT 1 cut(s) 659
TaqI TCGA 6 cut(s) 5, 1017, 1168, 1178, 1236, 1272
TasI AATT 8 cut(s) 464, 556, 580, 622, 764, 1329, 1387, 1397
TatI WGTACW 1 cut(s) 1073
TfiI GAWTC 5 cut(s) 142, 975, 1233, 1356, 1379
Tru1I TTAA 1 cut(s) 1454
Tru9I TTAA 1 cut(s) 1454
TscAI CASTG 5 cut(s) 412, 603, 849, 917, 1547
TseFI GTSAC 3 cut(s) 236, 955, 1490
TseI GCWGC 3 cut(s) 784, 814, 1653
Tsp45I GTSAC 3 cut(s) 236, 955, 1490
TspDTI ATGAA 5 cut(s) 9, 565, 593, 826, 1625
TspGWI ACGGA 1 cut(s) 1636
TspRI CASTG 5 cut(s) 412, 603, 849, 917, 1547
Van91I CCANNNNNTGG 1 cut(s) 277
VneI GTGCAC 1 cut(s) 1562
XapI RAATTY 2 cut(s) 580, 1397
XceI RCATGY 2 cut(s) 376, 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XhoI CTCGAG 1 cut(s) 1167
XmaJI CCTAGG 1 cut(s) 512
XmiI GTMKAC 3 cut(s) 5, 193, 333
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 3 cut(s) 513, 891, 1436
Zsp2I ATGCAT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.