Rmu_sc0001434.1_g000005

Ubiquitinyl hydrolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001434.1
OG0012946
Physical Location & Seq
Reverse (-)
28998 .. 31645
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001434.1_g000005.1.cds

Sequence Viewer

Length: 1767 bp
atgtcgacaagaaaaccttcatacacaaacccaagaccctgcaagcacctttcagcttacaagctcaaacatggcctgagtgggtataaatcactccaaaagtgcctcaagaccaccccgaatggaaggacaagtgttgagaatccggccacaaagatagccagatgcacttcctgtaatgggtatcaaggtagactctatttttgtctgatttgctcttcaatctcatgctcaggtcacacccttttgcacacccagtctgagattggccatgatttagcggtggacattgaaagggctgagctttattgctgtgcctgctctgatcaggtctacgatcctgattttgataagactgtcatgtctaaacacatgttggacttgagttatagcacaaacagaaacagtgcagatagtataggggagaggctgtgtgagaggaagaggctgggttctggggtagaattggcagatttgaacaagtcaggacagctggctttgatgagagatcatagggctaagtcatgttatccattaggtttgaggggactcaataatctgggcaatacttgtttcatgaattctgtgctgcaagcactgttgcatgctcctccaatgagaaattacttcttgagtgatcggcataaccgcaggacgtgcaggaagaggtcggccaaccggctctgtctcccctgtgagcttgatggtatcttctccgctgtctactcgggtggtggtgatcgcactccttatagcccggctcaatttctttacagttggtggcagcattcagaaaatctggcaagttatgagcagcaggatgctcatgagtttttcatttcagtgttagatgggatccatgagagagaaagcaaagcaaggaaccaaactagagatagtggagattgccagtgtattactcatagggtcttctctggacagttgagatcagatgtcacttgtatgacttgtggattcacttcaacaacttatgacccttgtttggacatatcgctcgacttggacacaaaccattgctctaataagcctgtcaaacgaaatgacaacagcagtacatctactcttcttggttgtttagacttgttcaccaaaccagagaagttgggaacagaccagaagctgttttgtcaaaactgtcaagaaccgcgagactcatcgaagcaactgtctattagaaagctccctttggtgttgtgtttgcatataaaacgattcgagcactccctggtcagaaagatgatgaggaaaatcgacagctatatgcattttcctttctccttagacatgactccatatctgtcctcttcaattgtcagaaacagatttgggaatagaatctttgcctttgagggtgatgattccgataattctacagaatttgagatttttgcagttattactcattctgggacgctagactctggccattatttaacttatttacgcataaaagagcaatggtataagtgtgacaatgcttggattagtgaggttgatgagagggttgtaagagcttcacagtgctatatgatgttttatgtgcacaagacgctttactataaagcaaaagaagatttgagttgcttgccagtttccacacggaaggaggcttttcataggtcaaggcaggctgggcacgaatccacggagatggtgaaaatgaagactgccactgctgaagaaacttttgcaggaatgagatccttctggcctggaggaagtcataaatccatattgagcacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

588

Amino Acids

66.91

Weight (kDa)

8.68

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10790 AT5G10790
fragaria_vesca FvH4_2g41240
malus_domestica MD08G1052900.v1.1 MD15G1042700.v1.1
prunus_persica Prupe.1G396000_v2.0.a1
pyrus_communis pycom08g04410 pycom15g03980
rosa_chinensis RchiOBHm_Chr6g0299261
rosa_laevigata RLG00000011448
rosa_multiflora Rmu_sc0001434.1_g000005
rosa_roxburghii Rroxscaffold_7G00168850
rosa_rugosa Rorug06G0286700
rosa_samantha Rh6AG398000 Rh6BG406500 Rh6CG412600 Rh6DG399300
rosa_wichuraiana Rw6G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 361
AccI GTMKAC 4 cut(s) 5, 193, 333, 723
AccII CGCG 1 cut(s) 1168
AciI CCGC 4 cut(s) 281, 649, 717, 1166
AclWI GGATC 4 cut(s) 332, 850, 863, 1716
AcoI YGGCCR 4 cut(s) 147, 268, 672, 1444
AcsI RAATTY 2 cut(s) 580, 1397
AcuI CTGAAG 1 cut(s) 1719
AfaI GTAC 1 cut(s) 1075
AfiI CCNNNNNNNGG 2 cut(s) 180, 1003
AflIII ACRYGT 1 cut(s) 372
AgsI TTSAA 5 cut(s) 222, 293, 478, 984, 1329
AjiI CACGTC 1 cut(s) 657
AjnI CCWGG 2 cut(s) 1245, 1732
AluBI AGCT 9 cut(s) 56, 64, 304, 493, 700, 1141, 1201, 1278, 1535
AluI AGCT 9 cut(s) 56, 64, 304, 493, 700, 1141, 1201, 1278, 1535
Alw21I GWGCWC 3 cut(s) 1242, 1566, 1763
Alw26I GTCTC 2 cut(s) 692, 1164
Alw44I GTGCAC 1 cut(s) 1562
AlwI GGATC 4 cut(s) 332, 850, 863, 1716
AlwNI CAGNNNCTG 1 cut(s) 1141
Ama87I CYCGRG 1 cut(s) 727
AoxI GGCC 6 cut(s) 73, 147, 268, 672, 1444, 1730
ApaLI GTGCAC 1 cut(s) 1562
ApeKI GCWGC 3 cut(s) 589, 784, 814
ApoI RAATTY 2 cut(s) 580, 1397
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 1172, 1204
Asp700I GAANNNNTTC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 4 cut(s) 749, 1099, 1385, 1687
AvaI CYCGRG 1 cut(s) 727
BaeGI GKGCMC 2 cut(s) 1566, 1659
BalI TGGCCA 2 cut(s) 270, 1446
BamHI GGATCC 1 cut(s) 855
BbsI GAAGAC 2 cut(s) 922, 1691
Bbv12I GWGCWC 3 cut(s) 1242, 1566, 1763
BbvI GCAGC 3 cut(s) 576, 796, 826
BccI CCATC 3 cut(s) 698, 845, 1666
BciT130I CCWGG 2 cut(s) 1247, 1734
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 2 cut(s) 692, 1164
BfaI CTAG 2 cut(s) 891, 1436
BfmI CTRYAG 1 cut(s) 1392
BisI GCNGC 3 cut(s) 590, 785, 815
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 3 cut(s) 591, 786, 816
Bme1390I CCNGG 3 cut(s) 758, 1247, 1734
BmeT110I CYCGRG 1 cut(s) 727
BmgBI CACGTC 1 cut(s) 657
BmiI GGNNCC 2 cut(s) 857, 884
BmrFI CCNGG 3 cut(s) 758, 1247, 1734
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 155, 811
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 2 cut(s) 922, 1691
BpmI CTGGAG 1 cut(s) 1755
Bpu10I CCTNAGC 1 cut(s) 232
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 3 cut(s) 92, 403, 652
BpuMI CCSGG 1 cut(s) 758
BsaJI CCNNGG 2 cut(s) 1245, 1665
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 1003
Bse118I RCCGGY 1 cut(s) 678
Bse1I ACTGG 3 cut(s) 256, 910, 1610
Bse3DI GCAATG 2 cut(s) 1033, 1484
BseBI CCWGG 2 cut(s) 1247, 1734
BseDI CCNNGG 2 cut(s) 1245, 1665
BseGI GGATG 1 cut(s) 826
BseLI CCNNNNNNNGG 2 cut(s) 180, 1003
BseMI GCAATG 2 cut(s) 1033, 1484
BseMII CTCAG 4 cut(s) 68, 246, 252, 291
BseNI ACTGG 3 cut(s) 256, 910, 1610
BseRI GAGGAG 1 cut(s) 600
BseSI GKGCMC 2 cut(s) 1566, 1659
BseXI GCAGC 3 cut(s) 576, 796, 826
BseYI CCCAGC 2 cut(s) 448, 1652
BsgI GTGCAG 2 cut(s) 429, 679
Bsh1236I CGCG 1 cut(s) 1168
BshFI GGCC 6 cut(s) 75, 149, 270, 674, 1446, 1732
BsiHKAI GWGCWC 3 cut(s) 1242, 1566, 1763
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 3 cut(s) 146, 679, 758
BslFI GGGAC 2 cut(s) 561, 1444
BslI CCNNNNNNNGG 2 cut(s) 180, 1003
BsmAI GTCTC 2 cut(s) 692, 1164
BsmFI GGGAC 2 cut(s) 561, 1444
BsmI GAATGC 1 cut(s) 787
BsnI GGCC 6 cut(s) 75, 149, 270, 674, 1446, 1732
BsoBI CYCGRG 1 cut(s) 727
Bsp1286I GDGCHC 4 cut(s) 1242, 1566, 1659, 1763
Bsp143I GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 4 cut(s) 281, 649, 717, 1166
BspANI GGCC 6 cut(s) 75, 149, 270, 674, 1446, 1732
BspCNI CTCAG 4 cut(s) 69, 245, 253, 292
BspFNI CGCG 1 cut(s) 1168
BspHI TCATGA 2 cut(s) 576, 826
BspLI GGNNCC 2 cut(s) 857, 884
BspPI GGATC 4 cut(s) 332, 850, 863, 1716
BspQI GCTCTTC 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 1033, 1484
BsrFI RCCGGY 1 cut(s) 678
BsrI ACTGG 3 cut(s) 256, 910, 1610
BssAI RCCGGY 1 cut(s) 678
BssECI CCNNGG 2 cut(s) 1245, 1665
BssMI GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
Bst2UI CCWGG 2 cut(s) 1247, 1734
Bst4CI ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
Bst6I CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
BstAPI GCANNNNNTGC 1 cut(s) 657
BstC8I GCNNGC 7 cut(s) 44, 319, 495, 594, 606, 1607, 1650
BstDEI CTNAG 6 cut(s) 77, 232, 261, 300, 519, 1300
BstDSI CCRYGG 1 cut(s) 1665
BstF5I GGATG 1 cut(s) 826
BstFNI CGCG 1 cut(s) 1168
BstKTI GATC 8 cut(s) 328, 340, 511, 640, 742, 858, 950, 1724
BstMAI GTCTC 2 cut(s) 692, 1164
BstMBI GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
BstMWI GCNNNNNNNGC 4 cut(s) 318, 657, 1570, 1654
BstNI CCWGG 2 cut(s) 1247, 1734
BstNSI RCATGY 2 cut(s) 376, 608
BstSCI CCNGG 3 cut(s) 756, 1245, 1732
BstSFI CTRYAG 1 cut(s) 1392
BstSLI GKGCMC 2 cut(s) 1566, 1659
BstUI CGCG 1 cut(s) 1168
BstV1I GCAGC 3 cut(s) 576, 796, 826
BstV2I GAAGAC 2 cut(s) 922, 1691
BstX2I RGATCY 2 cut(s) 855, 1721
BstXI CCANNNNNNTGG 1 cut(s) 1672
BstYI RGATCY 2 cut(s) 855, 1721
BsuRI GGCC 6 cut(s) 75, 149, 270, 674, 1446, 1732
BtgI CCRYGG 1 cut(s) 1665
BtrI CACGTC 1 cut(s) 657
BtsCI GGATG 1 cut(s) 826
BtsI GCAGTG 1 cut(s) 1692
BtsIMutI CAGTG 6 cut(s) 412, 596, 849, 917, 1547, 1692
Cac8I GCNNGC 7 cut(s) 44, 319, 495, 594, 606, 1607, 1650
CaiI CAGNNNCTG 1 cut(s) 1141
CciI TCATGA 2 cut(s) 576, 826
Cfr10I RCCGGY 1 cut(s) 678
CseI GACGC 2 cut(s) 1441, 1579
Csp6I GTAC 1 cut(s) 1074
CviQI GTAC 1 cut(s) 1074
DdeI CTNAG 6 cut(s) 77, 232, 261, 300, 519, 1300
DpnI GATC 8 cut(s) 327, 339, 510, 639, 741, 857, 949, 1723
DpnII GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
DrdI GACNNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 4 cut(s) 147, 268, 672, 1444
Eam1104I CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
EarI CTCTTC 5 cut(s) 223, 437, 659, 1089, 1330
Eco57I CTGAAG 1 cut(s) 1719
Eco88I CYCGRG 1 cut(s) 727
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 2 cut(s) 1245, 1732
EcoT22I ATGCAT 1 cut(s) 1287
FalI AAGNNNNNCTT 1 cut(s) 33
FaqI GGGAC 2 cut(s) 561, 1444
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 4 cut(s) 5, 193, 333, 723
Fnu4HI GCNGC 3 cut(s) 590, 785, 815
FokI GGATG 1 cut(s) 833
Fsp4HI GCNGC 3 cut(s) 590, 785, 815
FspBI CTAG 2 cut(s) 891, 1436
GluI GCNGC 3 cut(s) 590, 785, 815
GsaI CCCAGC 2 cut(s) 452, 1656
GsuI CTGGAG 1 cut(s) 1755
HaeIII GGCC 6 cut(s) 75, 149, 270, 674, 1446, 1732
HapII CCGG 3 cut(s) 146, 679, 758
HgaI GACGC 2 cut(s) 1441, 1579
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HpaII CCGG 3 cut(s) 146, 679, 758
HphI GGTGA 4 cut(s) 749, 1099, 1385, 1687
Hpy166II GTNNAC 7 cut(s) 6, 194, 286, 334, 724, 1107, 1564
Hpy188I TCNGA 8 cut(s) 210, 262, 325, 793, 952, 1253, 1337, 1384
Hpy188III TCNNGA 8 cut(s) 109, 341, 486, 577, 631, 827, 936, 1160
Hpy8I GTNNAC 7 cut(s) 6, 194, 286, 334, 724, 1107, 1564
HpyAV CCTTC 4 cut(s) 27, 120, 1618, 1735
HpyCH4III ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
HpyCH4IV ACGT 1 cut(s) 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 657, 1570, 1654
HpyF3I CTNAG 6 cut(s) 77, 232, 261, 300, 519, 1300
HpySE526I ACGT 1 cut(s) 656
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 613, 1206
Lsp1109I GCAGC 3 cut(s) 576, 796, 826
LweI GCATC 2 cut(s) 155, 811
MaeI CTAG 2 cut(s) 891, 1436
MaeII ACGT 1 cut(s) 656
MaeIII GTNAC 3 cut(s) 236, 955, 1490
MalI GATC 8 cut(s) 327, 339, 510, 639, 741, 857, 949, 1723
MboI GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 2 cut(s) 855, 1721
MhlI GDGCHC 4 cut(s) 1242, 1566, 1659, 1763
MlsI TGGCCA 2 cut(s) 270, 1446
MluCI AATT 7 cut(s) 464, 580, 622, 764, 1329, 1387, 1397
MluNI TGGCCA 2 cut(s) 270, 1446
MlyI GAGTC 5 cut(s) 189, 543, 1166, 1303, 1433
MmeI TCCRAC 1 cut(s) 357
Mox20I TGGCCA 2 cut(s) 270, 1446
Mph1103I ATGCAT 1 cut(s) 1287
MroXI GAANNNNTTC 1 cut(s) 16
MscI TGGCCA 2 cut(s) 270, 1446
MseI TTAA 1 cut(s) 1454
MslI CAYNNNNRTG 3 cut(s) 842, 962, 1670
Msp20I TGGCCA 2 cut(s) 270, 1446
MspA1I CMGCKG 2 cut(s) 493, 719
MspI CCGG 3 cut(s) 146, 679, 758
MspR9I CCNGG 3 cut(s) 758, 1247, 1734
MunI CAATTG 1 cut(s) 1329
Mva1269I GAATGC 1 cut(s) 787
MvaI CCWGG 2 cut(s) 1247, 1734
MvnI CGCG 1 cut(s) 1168
MwoI GCNNNNNNNGC 4 cut(s) 318, 657, 1570, 1654
NciI CCSGG 1 cut(s) 758
NdeII GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
NlaIV GGNNCC 2 cut(s) 857, 884
NmuCI GTSAC 3 cut(s) 236, 955, 1490
NsiI ATGCAT 1 cut(s) 1287
NspI RCATGY 2 cut(s) 376, 608
PaeI GCATGC 1 cut(s) 608
PagI TCATGA 2 cut(s) 576, 826
PciI ACATGT 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 223
PctI GAATGC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 6 cut(s) 142, 975, 1233, 1356, 1379, 1661
PkrI GCNGC 3 cut(s) 591, 786, 816
PleI GAGTC 5 cut(s) 189, 543, 1166, 1303, 1433
PpsI GAGTC 5 cut(s) 189, 543, 1166, 1303, 1433
PscI ACATGT 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 1245, 1732
PspFI CCCAGC 2 cut(s) 448, 1652
PspGI CCWGG 2 cut(s) 1245, 1732
PspN4I GGNNCC 2 cut(s) 857, 884
PstNI CAGNNNCTG 1 cut(s) 1141
PsuI RGATCY 2 cut(s) 855, 1721
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 1 cut(s) 1075
RsaNI GTAC 1 cut(s) 1074
RseI CAYNNNNRTG 3 cut(s) 842, 962, 1670
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 1454
SatI GCNGC 3 cut(s) 590, 785, 815
Sau3AI GATC 8 cut(s) 325, 337, 508, 637, 739, 855, 947, 1721
SchI GAGTC 5 cut(s) 189, 543, 1166, 1303, 1433
ScrFI CCNGG 3 cut(s) 758, 1247, 1734
SduI GDGCHC 4 cut(s) 1242, 1566, 1659, 1763
SfaNI GCATC 2 cut(s) 155, 811
SfcI CTRYAG 1 cut(s) 1392
SmiMI CAYNNNNRTG 3 cut(s) 842, 962, 1670
SmlI CTYRAG 3 cut(s) 107, 382, 631
SmoI CTYRAG 3 cut(s) 107, 382, 631
SphI GCATGC 1 cut(s) 608
Sse9I AATT 7 cut(s) 464, 580, 622, 764, 1329, 1387, 1397
SsiI CCGC 4 cut(s) 281, 649, 717, 1166
SspMI CTAG 2 cut(s) 891, 1436
StyD4I CCNGG 3 cut(s) 756, 1245, 1732
TaaI ACNGT 8 cut(s) 358, 407, 600, 776, 942, 1157, 1188, 1542
TaiI ACGT 1 cut(s) 659
TaqI TCGA 5 cut(s) 5, 1017, 1178, 1236, 1272
TasI AATT 7 cut(s) 464, 580, 622, 764, 1329, 1387, 1397
TatI WGTACW 1 cut(s) 1073
TfiI GAWTC 6 cut(s) 142, 975, 1233, 1356, 1379, 1661
Tru1I TTAA 1 cut(s) 1454
Tru9I TTAA 1 cut(s) 1454
TscAI CASTG 6 cut(s) 412, 603, 849, 917, 1547, 1699
TseFI GTSAC 3 cut(s) 236, 955, 1490
TseI GCWGC 3 cut(s) 589, 784, 814
Tsp45I GTSAC 3 cut(s) 236, 955, 1490
TspDTI ATGAA 6 cut(s) 9, 565, 593, 826, 1625, 1697
TspGWI ACGGA 2 cut(s) 1636, 1682
TspRI CASTG 6 cut(s) 412, 603, 849, 917, 1547, 1699
VneI GTGCAC 1 cut(s) 1562
XapI RAATTY 2 cut(s) 580, 1397
XceI RCATGY 2 cut(s) 376, 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmiI GTMKAC 4 cut(s) 5, 193, 333, 723
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 2 cut(s) 891, 1436
Zsp2I ATGCAT 1 cut(s) 1287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.