Prupe.1G396000_v2.0.a1

Ubiquitinyl hydrolase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0012946
Physical Location & Seq
Reverse (-)
35133624 .. 35136627
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G396000.1

Sequence Viewer

Length: 1662 bp
ATGTCGACTAGAAAACCTTCATACACAAACCCAAAACCCTGCAAGCACCTTTCAGCTTACAAGCTCAAACATGGCTTGAGTGGCTATAAATCACTCCAAAAGTGCCTCAAGACCACCCCAAATGGAAGGACAAGTGTTCAGAATCCTGGCACAGAGATACCCAGATGCAGTTCCTGTAATGGGTATCAAGGTAGACTCTATATTTGTTTGATTTGCTCTTCAATCTCATGCTCAAGCCACGCCCTTTTGCATACCCAGTCTGAGATTGGCCACTATTTAGCTGTGGACATTGAAAGGGCTGAGCTTTATTGCTGCGCGTGCTCTGATCAGGTCTATGATCCTGATTTTGATAAGACTGTGATGTCTAAACACATGGTGGACTTGCCATATAGCACAAATAGTGCAGACAGTATAGGAGAGAGACTGTGTGAAAGGAAGAGGCTGGGTTCAGGTGTAGAATTAGTGGATTTGAAGAAGGCCGAAAAGTCGGCTTTGACGAGAGATCAGAGGGCTAAATCATGTTACCCATTGGGTTTGAGGGGATTGACTAATTTGGGCAATACTTGTTTCATGAATTCTGTGTTGCAAGCACTACTACATGCACCTCCTTTGAGAAATTACTTCTTGAGTGATCGGCATAACCCCAAAACATGCCGGAAAAGGACTGCGAATCGGCTGTGTTTGCCTTGCGAACTTCATGGTATTTTCTCGGCTGTGTATTTGGGGGATCGGACTCCTTATAGCCCGGCTCAATTTCTTTACAGTTGGTGGCAGCATTCAGAAAATCTGGCAAGTTATGAGCAGCAGGATGCTCATGAGTTCTTCATTTCAGTCTTAGATGGGATCCATGAGAAAGAAAGCAAAGCAAGGAACCAAACTAGAGATACCGGAGATTGCCAGTGTATTGCTCATAGGGTATTCTCAGGACTGTTGAGGTCCGATGTCACTTGTATGACTTGTGGATTCACCTCAACAACTTATGACCCTTGTTTGGACATATCACTCAACTTAGACACAAGCCATTGTTCTTGGTCAGATGCATCTAATAAGCCCGTCAAACCAAATGACAACAGCAGTTCATCTACTCTTTTAGGTTGTTTAGACTTGTTTACAAAACCAGAGAAGTTGGGATCAGACCAAAAACTGTACTGTCAGAACTGTGAAGAATTGCGAGACTCATCGAAGCAAATGTCCATCAGAAAGCTCCCGTTGGTGTTGTGTTTGCATATAAAACGATTTGAGCACTCCCTTGTCAGAAAAATGATGAGGAAAATTGACAGCTATTTGCATTTTCCTTTCTCCCTAGACATGACTCCATATCTGTCTTCTTCAATTGTCAGAAACAGATTTGGAAATAGGATCTTTGCTTTTGAGGGGGATGAATCTGATATATCTACAGAATTTGAGATTTTTGCAGTAGTTACTCATTCTGGGACACTAGAATCTGGCCATTATGTGACGTATTTACGCATAAAAAACCAGTGGTACAAATGTGACAATGCTTGGATTAATGAGGTTGACGAGGGAATTGTAAGAGCTTCACAGTGTTATATGATGTTCTATGTGCACAAGATGCTTTACTATAAATCACACGAAGATTTGAGTCGCCTGCCCATTTCCACACGGATAGAGGCATTTCACAGGGTAGCTGGTTGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UCH zf-UBP

Protein Analysis

554

Amino Acids

62.9

Weight (kDa)

8.44

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012946)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10790 AT5G10790
fragaria_vesca FvH4_2g41240
malus_domestica MD08G1052900.v1.1 MD15G1042700.v1.1
prunus_persica Prupe.1G396000_v2.0.a1
pyrus_communis pycom08g04410 pycom15g03980
rosa_chinensis RchiOBHm_Chr6g0299261
rosa_laevigata RLG00000011448
rosa_multiflora Rmu_sc0001434.1_g000005
rosa_roxburghii Rroxscaffold_7G00168850
rosa_rugosa Rorug06G0286700
rosa_samantha Rh6AG398000 Rh6BG406500 Rh6CG412600 Rh6DG399300
rosa_wichuraiana Rw6G034840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 361
AccI GTMKAC 2 cut(s) 5, 193
AccII CGCG 1 cut(s) 317
AclWI GGATC 6 cut(s) 332, 735, 838, 851, 1138, 1367
AcoI YGGCCR 2 cut(s) 268, 1447
AcsI RAATTY 2 cut(s) 574, 1400
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 2 cut(s) 1148, 1487
AfiI CCNNNNNNNGG 2 cut(s) 180, 991
AgsI TTSAA 4 cut(s) 222, 293, 472, 1332
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 8 cut(s) 56, 64, 281, 304, 1204, 1281, 1538, 1649
AluI AGCT 8 cut(s) 56, 64, 281, 304, 1204, 1281, 1538, 1649
Alw21I GWGCWC 3 cut(s) 323, 1245, 1569
Alw26I GTCTC 2 cut(s) 415, 1167
Alw44I GTGCAC 1 cut(s) 1565
AlwI GGATC 6 cut(s) 332, 735, 838, 851, 1138, 1367
AlwNI CAGNNNCTG 1 cut(s) 174
AoxI GGCC 3 cut(s) 268, 477, 1447
ApaLI GTGCAC 1 cut(s) 1565
ApeKI GCWGC 4 cut(s) 312, 772, 802, 1656
ApoI RAATTY 2 cut(s) 574, 1400
ArsI GACNNNNNNTTYG 4 cut(s) 186, 218, 1175, 1207
AseI ATTAAT 1 cut(s) 1509
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 936
AsuC2I CCSGG 1 cut(s) 746
AsuHPI GGTGA 1 cut(s) 958
AvaII GGWCC 1 cut(s) 936
BaeGI GKGCMC 1 cut(s) 1569
BaeI ACNNNNGTAYC 2 cut(s) 1477, 1510
BalI TGGCCA 2 cut(s) 270, 1449
BamHI GGATCC 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 1317
Bbv12I GWGCWC 3 cut(s) 323, 1245, 1569
BbvI GCAGC 4 cut(s) 299, 784, 814, 1643
BccI CCATC 2 cut(s) 833, 1202
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 1 cut(s) 746
BcoDI GTCTC 2 cut(s) 415, 1167
BfaI CTAG 4 cut(s) 9, 879, 1304, 1439
BfmI CTRYAG 1 cut(s) 1395
BisI GCNGC 4 cut(s) 313, 773, 803, 1657
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 4 cut(s) 314, 774, 804, 1658
Bme1390I CCNGG 2 cut(s) 147, 746
Bme18I GGWCC 1 cut(s) 936
BmgT120I GGNCC 1 cut(s) 936
BmiI GGNNCC 2 cut(s) 845, 872
BmrFI CCNGG 2 cut(s) 147, 746
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 5 cut(s) 155, 799, 1027, 1049, 1563
BmuI ACTGGG 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 1317
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 4 cut(s) 92, 97, 217, 646
BpuMI CCSGG 1 cut(s) 746
BsaWI WCCGGW 1 cut(s) 887
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 991
Bse1I ACTGG 3 cut(s) 256, 898, 1480
BseBI CCWGG 1 cut(s) 147
BseGI GGATG 2 cut(s) 814, 1384
BseLI CCNNNNNNNGG 2 cut(s) 180, 991
BseMII CTCAG 3 cut(s) 252, 291, 936
BseNI ACTGG 3 cut(s) 256, 898, 1480
BseSI GKGCMC 1 cut(s) 1569
BseXI GCAGC 4 cut(s) 299, 784, 814, 1643
BseYI CCCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 3 cut(s) 270, 479, 1449
BsiHKAI GWGCWC 3 cut(s) 323, 1245, 1569
BsiSI CCGG 3 cut(s) 655, 746, 888
BslFI GGGAC 1 cut(s) 1447
BslI CCNNNNNNNGG 2 cut(s) 180, 991
BsmAI GTCTC 2 cut(s) 415, 1167
BsmFI GGGAC 1 cut(s) 1447
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 3 cut(s) 270, 479, 1449
Bsp1286I GDGCHC 3 cut(s) 323, 1245, 1569
Bsp143I GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
Bsp1720I GCTNAGC 1 cut(s) 300
BspANI GGCC 3 cut(s) 270, 479, 1449
BspCNI CTCAG 3 cut(s) 253, 292, 935
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 2 cut(s) 570, 814
BspLI GGNNCC 2 cut(s) 845, 872
BspPI GGATC 6 cut(s) 332, 735, 838, 851, 1138, 1367
BspQI GCTCTTC 1 cut(s) 223
BsrI ACTGG 3 cut(s) 256, 898, 1480
BssMI GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 9 cut(s) 358, 410, 426, 764, 930, 1146, 1151, 1160, 1545
Bst6I CTCTTC 2 cut(s) 223, 431
BstAPI GCANNNNNTGC 1 cut(s) 1573
BstC8I GCNNGC 4 cut(s) 44, 319, 588, 1610
BstDEI CTNAG 5 cut(s) 261, 300, 835, 922, 1009
BstF5I GGATG 2 cut(s) 814, 1384
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 8 cut(s) 328, 340, 505, 634, 730, 846, 1133, 1362
BstMAI GTCTC 2 cut(s) 415, 1167
BstMBI GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
BstMWI GCNNNNNNNGC 4 cut(s) 81, 318, 682, 1573
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 2 cut(s) 602, 654
BstSCI CCNGG 2 cut(s) 145, 744
BstSFI CTRYAG 1 cut(s) 1395
BstSLI GKGCMC 1 cut(s) 1569
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 4 cut(s) 299, 784, 814, 1643
BstV2I GAAGAC 1 cut(s) 1317
BstX2I RGATCY 2 cut(s) 843, 1359
BstYI RGATCY 2 cut(s) 843, 1359
BsuRI GGCC 3 cut(s) 270, 479, 1449
BtsCI GGATG 2 cut(s) 814, 1384
BtsIMutI CAGTG 3 cut(s) 905, 1487, 1550
Cac8I GCNNGC 4 cut(s) 44, 319, 588, 1610
CaiI CAGNNNCTG 1 cut(s) 174
CciI TCATGA 2 cut(s) 570, 814
CfoI GCGC 1 cut(s) 317
Cfr13I GGNCC 1 cut(s) 936
Csp6I GTAC 2 cut(s) 1147, 1486
CviQI GTAC 2 cut(s) 1147, 1486
DdeI CTNAG 5 cut(s) 261, 300, 835, 922, 1009
DpnI GATC 8 cut(s) 327, 339, 504, 633, 729, 845, 1132, 1361
DpnII GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
DraIII CACNNNGTG 1 cut(s) 376
DrdI GACNNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 361
EaeI YGGCCR 2 cut(s) 268, 1447
Eam1104I CTCTTC 2 cut(s) 223, 431
EarI CTCTTC 2 cut(s) 223, 431
Eco47I GGWCC 1 cut(s) 936
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 1042
FaqI GGGAC 1 cut(s) 1447
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 2 cut(s) 5, 193
Fnu4HI GCNGC 4 cut(s) 313, 773, 803, 1657
FokI GGATG 2 cut(s) 821, 1391
Fsp4HI GCNGC 4 cut(s) 313, 773, 803, 1657
FspBI CTAG 4 cut(s) 9, 879, 1304, 1439
GlaI GCGC 1 cut(s) 316
GluI GCNGC 4 cut(s) 313, 773, 803, 1657
GsaI CCCAGC 1 cut(s) 446
HaeIII GGCC 3 cut(s) 270, 479, 1449
HapII CCGG 3 cut(s) 655, 746, 888
HhaI GCGC 1 cut(s) 317
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 2 cut(s) 6, 1519
HindII GTYRAC 2 cut(s) 6, 1519
HpaII CCGG 3 cut(s) 655, 746, 888
HphI GGTGA 1 cut(s) 958
Hpy166II GTNNAC 7 cut(s) 6, 194, 286, 379, 1110, 1519, 1567
Hpy188III TCNNGA 6 cut(s) 109, 341, 571, 625, 815, 924
Hpy8I GTNNAC 7 cut(s) 6, 194, 286, 379, 1110, 1519, 1567
HpyAV CCTTC 3 cut(s) 27, 120, 469
HpyCH4III ACNGT 9 cut(s) 358, 410, 426, 764, 930, 1146, 1151, 1160, 1545
HpyCH4IV ACGT 1 cut(s) 1460
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 318, 682, 1573
HpyF3I CTNAG 5 cut(s) 261, 300, 835, 922, 1009
HpySE526I ACGT 1 cut(s) 1460
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 1 cut(s) 1209
Lsp1109I GCAGC 4 cut(s) 299, 784, 814, 1643
LweI GCATC 5 cut(s) 155, 799, 1027, 1049, 1563
MaeI CTAG 4 cut(s) 9, 879, 1304, 1439
MaeII ACGT 1 cut(s) 1460
MaeIII GTNAC 5 cut(s) 521, 943, 1420, 1456, 1493
MalI GATC 8 cut(s) 327, 339, 504, 633, 729, 845, 1132, 1361
MboI GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
MboII GAAGA 8 cut(s) 210, 448, 484, 814, 1175, 1317, 1320, 1607
MfeI CAATTG 1 cut(s) 1332
MflI RGATCY 2 cut(s) 843, 1359
MhlI GDGCHC 3 cut(s) 323, 1245, 1569
MlsI TGGCCA 2 cut(s) 270, 1449
MluNI TGGCCA 2 cut(s) 270, 1449
MlyI GAGTC 5 cut(s) 189, 727, 1169, 1306, 1612
Mox20I TGGCCA 2 cut(s) 270, 1449
Mph1103I ATGCAT 1 cut(s) 1042
MroXI GAANNNNTTC 1 cut(s) 16
MscI TGGCCA 2 cut(s) 270, 1449
MseI TTAA 1 cut(s) 1509
MslI CAYNNNNRTG 1 cut(s) 950
Msp20I TGGCCA 2 cut(s) 270, 1449
MspI CCGG 3 cut(s) 655, 746, 888
MspR9I CCNGG 2 cut(s) 147, 746
MunI CAATTG 1 cut(s) 1332
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 4 cut(s) 81, 318, 682, 1573
NciI CCSGG 1 cut(s) 746
NdeII GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
NlaIV GGNNCC 2 cut(s) 845, 872
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 3 cut(s) 943, 1456, 1493
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 602, 654
PagI TCATGA 2 cut(s) 570, 814
PciSI GCTCTTC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 494
PctI GAATGC 1 cut(s) 775
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 5 cut(s) 142, 670, 963, 1382, 1442
PkrI GCNGC 4 cut(s) 314, 774, 804, 1658
PleI GAGTC 5 cut(s) 189, 727, 1169, 1306, 1611
PpsI GAGTC 5 cut(s) 189, 727, 1169, 1306, 1611
PshBI ATTAAT 1 cut(s) 1509
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 442
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 2 cut(s) 845, 872
PspPI GGNCC 1 cut(s) 936
PstNI CAGNNNCTG 1 cut(s) 174
PsuI RGATCY 2 cut(s) 843, 1359
RsaI GTAC 2 cut(s) 1148, 1487
RsaNI GTAC 2 cut(s) 1147, 1486
RseI CAYNNNNRTG 1 cut(s) 950
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 1509
SatI GCNGC 4 cut(s) 313, 773, 803, 1657
Sau3AI GATC 8 cut(s) 325, 337, 502, 631, 727, 843, 1130, 1359
Sau96I GGNCC 1 cut(s) 936
SchI GAGTC 5 cut(s) 189, 727, 1169, 1306, 1612
ScrFI CCNGG 2 cut(s) 147, 746
SduI GDGCHC 3 cut(s) 323, 1245, 1569
SfaNI GCATC 5 cut(s) 155, 799, 1027, 1049, 1563
SfcI CTRYAG 1 cut(s) 1395
SinI GGWCC 1 cut(s) 936
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 4 cut(s) 76, 107, 232, 625
SmoI CTYRAG 4 cut(s) 76, 107, 232, 625
SspMI CTAG 4 cut(s) 9, 879, 1304, 1439
StyD4I CCNGG 2 cut(s) 145, 744
TaaI ACNGT 9 cut(s) 358, 410, 426, 764, 930, 1146, 1151, 1160, 1545
TaiI ACGT 1 cut(s) 1463
TaqI TCGA 2 cut(s) 5, 1181
TatI WGTACW 1 cut(s) 1146
TfiI GAWTC 5 cut(s) 142, 670, 963, 1382, 1442
Tru1I TTAA 1 cut(s) 1509
Tru9I TTAA 1 cut(s) 1509
TscAI CASTG 3 cut(s) 905, 1487, 1550
TseFI GTSAC 3 cut(s) 943, 1456, 1493
TseI GCWGC 4 cut(s) 312, 772, 802, 1656
Tsp45I GTSAC 3 cut(s) 943, 1456, 1493
TspDTI ATGAA 7 cut(s) 9, 559, 587, 686, 814, 1068, 1395
TspGWI ACGGA 1 cut(s) 1639
TspRI CASTG 3 cut(s) 905, 1487, 1550
VneI GTGCAC 1 cut(s) 1565
VpaK11BI GGWCC 1 cut(s) 936
VspI ATTAAT 1 cut(s) 1509
XapI RAATTY 2 cut(s) 574, 1400
XceI RCATGY 2 cut(s) 602, 654
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmiI GTMKAC 2 cut(s) 5, 193
XmnI GAANNNNTTC 1 cut(s) 16
XspI CTAG 4 cut(s) 9, 879, 1304, 1439
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.