MD08G1061700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0017731
Physical Location & Seq
Reverse (-)
4886691 .. 4887221
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1061700.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGAAGCAGCTGCCCCTGAGTTTCCTCCGCCGGAGCCTGCTGTCTCCGTCGACATCGCCGGAAAGTTTCCGGAGCTCAGTGACGAGCTCTTGCCGGAGATTCCCATCAAAGAAGAGTTGATTGAGGAGGTCATGCAAGAGCTCTACAAGGAGATAACGACCACCAACTCCCCCGCCGCTGCCCCCACCACTACAGCTACTTCTGATTGCAAAAAACCGTCTTCACCGGCGACATTTTCTACGCCGTCGCTGGTGGTCGGGAATGGGAAGAGCGAGAGCTGCGGGGCGTCAGTTTCTGACTCCGCATCAACGGTGATGGTTGGAGTGGAGTTTGTGGGGCCCGCCGGAAAAAAGGGTGGATCGCCGGAGAATGGGGCGTGGAGGAGTGAATGCGGGAGGAGCAATCATGGGTTGGTGGTTGTGAAGGGAGGAATGGACGGCTGTGATCATGCAGGGGAGGTTGATGATGAATGGCTGGAGAGAGTGCTGATGAGCACGTGGGGCCCGGTAGACCTTGATCCATGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

18.47

Weight (kDa)

4.29

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017731)

Species Orthologous Gene IDs
malus_domestica MD08G1061700.v1.1 MD15G1060000.v1.1
prunus_persica Prupe.1G411900_v2.0.a1
pyrus_communis pycom08g05120
rosa_chinensis RchiOBHm_Chr6g0310921
rosa_laevigata RLG00000010450
rosa_roxburghii Rroxscaffold_7G00158170
rosa_rugosa Rorug06G0384000
rosa_samantha Rh6AG497900 Rh6BG508300 Rh6CG514200 Rh6DG500600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 51, 510
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 7 cut(s) 29, 174, 177, 282, 303, 342, 393
AclWI GGATC 2 cut(s) 367, 512
AcvI CACGTG 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 287
AfiI CCNNNNNNNGG 1 cut(s) 371
AluBI AGCT 6 cut(s) 11, 76, 88, 142, 197, 279
AluI AGCT 6 cut(s) 11, 76, 88, 142, 197, 279
Alw21I GWGCWC 4 cut(s) 78, 90, 144, 497
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 2 cut(s) 367, 512
AlwNI CAGNNNCTG 1 cut(s) 296
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 2 cut(s) 338, 502
ApaI GGGCCC 2 cut(s) 342, 506
ApeKI GCWGC 4 cut(s) 8, 11, 179, 279
AspS9I GGNCC 4 cut(s) 338, 339, 502, 503
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 2 cut(s) 216, 325
BaeGI GKGCMC 2 cut(s) 342, 506
BanII GRGCYC 5 cut(s) 78, 90, 144, 342, 506
BbrPI CACGTG 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 213
Bbv12I GWGCWC 4 cut(s) 78, 90, 144, 497
BbvI GCAGC 3 cut(s) 20, 166, 266
BccI CCATC 2 cut(s) 113, 310
BceAI ACGGC 2 cut(s) 229, 454
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 49
BfmI CTRYAG 1 cut(s) 192
BisI GCNGC 5 cut(s) 9, 12, 177, 180, 280
BlsI GCNGC 5 cut(s) 10, 13, 178, 181, 281
Bme1390I CCNGG 1 cut(s) 506
BmgT120I GGNCC 4 cut(s) 338, 339, 502, 503
BmiI GGNNCC 5 cut(s) 36, 339, 340, 503, 504
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 506
BsaAI YACGTR 1 cut(s) 498
BsaBI GATNNNNATC 1 cut(s) 104
BsaHI GRCGYC 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 521
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse118I RCCGGY 1 cut(s) 226
Bse8I GATNNNNATC 1 cut(s) 104
BseAI TCCGGA 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 521
BseJI GATNNNNATC 1 cut(s) 104
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMII CTCAG 2 cut(s) 9, 91
BseRI GAGGAG 3 cut(s) 140, 397, 412
BseSI GKGCMC 2 cut(s) 342, 506
BseXI GCAGC 3 cut(s) 20, 166, 266
BshFI GGCC 2 cut(s) 340, 504
BsiHKAI GWGCWC 4 cut(s) 78, 90, 144, 497
BsiSI CCGG 8 cut(s) 32, 60, 71, 95, 227, 345, 365, 506
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 2 cut(s) 340, 504
Bsp120I GGGCCC 2 cut(s) 338, 502
Bsp1286I GDGCHC 6 cut(s) 78, 90, 144, 342, 497, 506
Bsp13I TCCGGA 1 cut(s) 70
Bsp143I GATC 3 cut(s) 359, 445, 517
Bsp19I CCATGG 1 cut(s) 521
BspACI CCGC 7 cut(s) 29, 174, 177, 282, 303, 342, 393
BspANI GGCC 2 cut(s) 340, 504
BspCNI CTCAG 2 cut(s) 10, 90
BspEI TCCGGA 1 cut(s) 70
BspLI GGNNCC 5 cut(s) 36, 339, 340, 503, 504
BspPI GGATC 2 cut(s) 367, 512
BspQI GCTCTTC 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 226
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 521
BssMI GATC 3 cut(s) 359, 445, 517
BssNI GRCGYC 1 cut(s) 287
BssT1I CCWWGG 1 cut(s) 521
Bst4CI ACNGT 2 cut(s) 219, 313
Bst6I CTCTTC 2 cut(s) 108, 263
BstACI GRCGYC 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 498
BstC8I GCNNGC 2 cut(s) 39, 342
BstDEI CTNAG 2 cut(s) 18, 77
BstDSI CCRYGG 1 cut(s) 521
BstKTI GATC 3 cut(s) 362, 448, 520
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 3 cut(s) 359, 445, 517
BstMWI GCNNNNNNNGC 3 cut(s) 279, 399, 501
BstSCI CCNGG 1 cut(s) 504
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 2 cut(s) 342, 506
BstV1I GCAGC 3 cut(s) 20, 166, 266
BstV2I GAAGAC 1 cut(s) 213
BsuRI GGCC 2 cut(s) 340, 504
BtgI CCRYGG 1 cut(s) 521
BtgZI GCGATG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 2 cut(s) 39, 342
CaiI CAGNNNCTG 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 4 cut(s) 338, 339, 502, 503
CseI GACGC 1 cut(s) 276
CviAII CATG 4 cut(s) 133, 407, 449, 522
DdeI CTNAG 2 cut(s) 18, 77
DpnI GATC 3 cut(s) 361, 447, 519
DpnII GATC 3 cut(s) 359, 445, 517
Eam1104I CTCTTC 2 cut(s) 108, 263
EarI CTCTTC 2 cut(s) 108, 263
EciI GGCGGA 1 cut(s) 18
Ecl136II GAGCTC 3 cut(s) 76, 88, 142
Eco130I CCWWGG 1 cut(s) 521
Eco24I GRGCYC 5 cut(s) 78, 90, 144, 342, 506
Eco53kI GAGCTC 3 cut(s) 76, 88, 142
Eco72I CACGTG 1 cut(s) 498
EcoICRI GAGCTC 3 cut(s) 76, 88, 142
EcoO109I RGGNCCY 2 cut(s) 338, 502
EcoT14I CCWWGG 1 cut(s) 521
EcoT38I GRGCYC 5 cut(s) 78, 90, 144, 342, 506
ErhI CCWWGG 1 cut(s) 521
FaeI CATG 4 cut(s) 136, 410, 452, 525
FaiI YATR 4 cut(s) 134, 408, 450, 523
FatI CATG 4 cut(s) 132, 406, 448, 521
FauI CCCGC 4 cut(s) 181, 275, 349, 386
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 2 cut(s) 51, 510
Fnu4HI GCNGC 5 cut(s) 9, 12, 177, 180, 280
FriOI GRGCYC 5 cut(s) 78, 90, 144, 342, 506
Fsp4HI GCNGC 5 cut(s) 9, 12, 177, 180, 280
GluI GCNGC 5 cut(s) 9, 12, 177, 180, 280
GsuI CTGGAG 1 cut(s) 497
HaeIII GGCC 2 cut(s) 340, 504
HapII CCGG 8 cut(s) 32, 60, 71, 95, 227, 345, 365, 506
HgaI GACGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 4 cut(s) 136, 410, 452, 525
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 2 cut(s) 100, 299
HpaII CCGG 8 cut(s) 32, 60, 71, 95, 227, 345, 365, 506
HphI GGTGA 2 cut(s) 216, 325
Hpy166II GTNNAC 2 cut(s) 52, 511
Hpy188I TCNGA 3 cut(s) 205, 298, 530
Hpy188III TCNNGA 2 cut(s) 71, 259
Hpy8I GTNNAC 2 cut(s) 52, 511
Hpy99I CGWCG 2 cut(s) 53, 250
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 219, 313
HpyCH4IV ACGT 1 cut(s) 497
HpyCH4V TGCA 3 cut(s) 136, 210, 452
HpyF10VI GCNNNNNNNGC 3 cut(s) 279, 399, 501
HpyF3I CTNAG 2 cut(s) 18, 77
HpySE526I ACGT 1 cut(s) 497
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 4 cut(s) 136, 410, 452, 525
Kpn2I TCCGGA 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 3 cut(s) 359, 445, 517
LguI GCTCTTC 1 cut(s) 263
LmnI GCTCC 3 cut(s) 34, 73, 399
Lsp1109I GCAGC 3 cut(s) 20, 166, 266
LweI GCATC 1 cut(s) 314
MaeII ACGT 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 80
MalI GATC 3 cut(s) 361, 447, 519
MboI GATC 3 cut(s) 359, 445, 517
MboII GAAGA 3 cut(s) 125, 213, 280
MhlI GDGCHC 6 cut(s) 78, 90, 144, 342, 497, 506
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 7 cut(s) 36, 118, 121, 375, 390, 422, 451
MroI TCCGGA 1 cut(s) 70
MspA1I CMGCKG 2 cut(s) 11, 179
MspI CCGG 8 cut(s) 32, 60, 71, 95, 227, 345, 365, 506
MspR9I CCNGG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 279, 399, 501
NciI CCSGG 1 cut(s) 506
NcoI CCATGG 1 cut(s) 521
NdeII GATC 3 cut(s) 359, 445, 517
NlaIII CATG 4 cut(s) 136, 410, 452, 525
NlaIV GGNNCC 5 cut(s) 36, 339, 340, 503, 504
NmuCI GTSAC 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 263
PctI GAATGC 1 cut(s) 395
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 5 cut(s) 10, 13, 178, 181, 281
PleI GAGTC 1 cut(s) 293
PmaCI CACGTG 1 cut(s) 498
PmlI CACGTG 1 cut(s) 498
PpsI GAGTC 1 cut(s) 293
Ppu21I YACGTR 1 cut(s) 498
Psp124BI GAGCTC 3 cut(s) 78, 90, 144
PspCI CACGTG 1 cut(s) 498
PspN4I GGNNCC 5 cut(s) 36, 339, 340, 503, 504
PspOMI GGGCCC 2 cut(s) 338, 502
PspPI GGNCC 4 cut(s) 338, 339, 502, 503
PstNI CAGNNNCTG 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 11
SacI GAGCTC 3 cut(s) 78, 90, 144
SalI GTCGAC 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 263
SatI GCNGC 5 cut(s) 9, 12, 177, 180, 280
Sau3AI GATC 3 cut(s) 359, 445, 517
Sau96I GGNCC 4 cut(s) 338, 339, 502, 503
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 6 cut(s) 78, 90, 144, 342, 497, 506
SfaNI GCATC 1 cut(s) 314
SfcI CTRYAG 1 cut(s) 192
SgrAI CRCCGGYG 1 cut(s) 226
SsiI CCGC 7 cut(s) 29, 174, 177, 282, 303, 342, 393
SstI GAGCTC 3 cut(s) 78, 90, 144
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 1 cut(s) 521
TaaI ACNGT 2 cut(s) 219, 313
TaiI ACGT 1 cut(s) 500
TaqI TCGA 1 cut(s) 51
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 4 cut(s) 8, 11, 179, 279
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 1 cut(s) 85
XmiI GTMKAC 2 cut(s) 51, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.