MD15G1060000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017731
Physical Location & Seq
Forward (+)
4011815 .. 4012333
519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1060000.v1.1.491

Sequence Viewer

Length: 519 bp
ATGGAAGCAGCTGCCCCAAAGTCTCCTCCGCCGGAGTCTGCCATCTCCGTCAAGATCGCCGGAAAGTTGCCGGAGCTCATTGACGAGCTCTTGCCGGAGATTCCCATTAAAGAAGAGTTGATTGAGGAGGTCATGCAAGAGCTCTACAAGGAGATAACCGCCGGTACCACCACCACTACAGCTACTTCTGATTGCACCAAACCGTCTTCACCGGCGGCGACATTTCCGACGCCGTCTCTGGTGGTCGGGAATGGGAAGAGCGAGAGCTGCGGGGCGTCGGTTTCTGACTCGGCGTCAACCGTGATGGCCGGCGTCGAGTTTGTCAGGCCCGCCAGAAAAACCGGGGGATCGCCGGAGAATGGGGCGTGTACGAGTGAATGCGGGTGGAGTAATAATGGGATGGTAGTTGGGAAGGAAGGAATGGACGGCTGTGATCATGCAGGGGAGGTTGATGATGAATGGCTGGAGAGAGTGCTGATGAGCACGTGGGGCCCGGTAGATCTTGATCCATGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

18.01

Weight (kDa)

4.27

Isoelectric Point (pI)

55.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017731)

Species Orthologous Gene IDs
malus_domestica MD08G1061700.v1.1 MD15G1060000.v1.1
prunus_persica Prupe.1G411900_v2.0.a1
pyrus_communis pycom08g05120
rosa_chinensis RchiOBHm_Chr6g0310921
rosa_laevigata RLG00000010450
rosa_roxburghii Rroxscaffold_7G00158170
rosa_rugosa Rorug06G0384000
rosa_samantha Rh6AG497900 Rh6BG508300 Rh6CG514200 Rh6DG500600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 164
AciI CCGC 6 cut(s) 29, 159, 215, 270, 330, 381
AclWI GGATC 2 cut(s) 355, 500
AcoI YGGCCR 1 cut(s) 306
AcvI CACGTG 1 cut(s) 486
AcyI GRCGYC 4 cut(s) 230, 275, 293, 312
AfaI GTAC 2 cut(s) 166, 370
AfiI CCNNNNNNNGG 1 cut(s) 359
AhdI GACNNNNNGTC 1 cut(s) 292
AluBI AGCT 6 cut(s) 11, 76, 88, 142, 182, 267
AluI AGCT 6 cut(s) 11, 76, 88, 142, 182, 267
Alw21I GWGCWC 4 cut(s) 78, 90, 144, 485
Alw26I GTCTC 2 cut(s) 27, 240
AlwI GGATC 2 cut(s) 355, 500
AoxI GGCC 3 cut(s) 306, 326, 490
ApaI GGGCCC 1 cut(s) 494
ApeKI GCWGC 3 cut(s) 8, 11, 267
Asp718I GGTACC 1 cut(s) 164
AspS9I GGNCC 3 cut(s) 327, 490, 491
AsuC2I CCSGG 2 cut(s) 343, 494
AsuHPI GGTGA 1 cut(s) 201
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 4 cut(s) 78, 90, 144, 494
BbrPI CACGTG 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 4 cut(s) 78, 90, 144, 485
BbvI GCAGC 2 cut(s) 20, 254
BccI CCATC 3 cut(s) 50, 298, 394
BceAI ACGGC 2 cut(s) 217, 442
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 2 cut(s) 343, 494
BcoDI GTCTC 2 cut(s) 27, 240
BfmI CTRYAG 1 cut(s) 177
BglII AGATCT 1 cut(s) 499
BisI GCNGC 4 cut(s) 9, 12, 216, 268
BlsI GCNGC 4 cut(s) 10, 13, 217, 269
Bme1390I CCNGG 2 cut(s) 343, 494
BmeRI GACNNNNNGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 327, 490, 491
BmiI GGNNCC 3 cut(s) 166, 491, 492
BmrFI CCNGG 2 cut(s) 343, 494
BpiI GAAGAC 1 cut(s) 198
BpmI CTGGAG 1 cut(s) 485
BpuMI CCSGG 2 cut(s) 343, 494
BsaAI YACGTR 1 cut(s) 486
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 4 cut(s) 230, 275, 293, 312
BsaJI CCNNGG 2 cut(s) 342, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse118I RCCGGY 3 cut(s) 161, 211, 308
Bse8I GATNNNNATC 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 342, 509
BseGI GGATG 1 cut(s) 405
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 359
BseRI GAGGAG 2 cut(s) 15, 140
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 2 cut(s) 20, 254
BshFI GGCC 3 cut(s) 308, 328, 492
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 4 cut(s) 78, 90, 144, 485
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 2 cut(s) 27, 240
BsmBI CGTCTC 1 cut(s) 240
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 3 cut(s) 308, 328, 492
Bsp120I GGGCCC 1 cut(s) 490
Bsp1286I GDGCHC 5 cut(s) 78, 90, 144, 485, 494
Bsp143I GATC 5 cut(s) 54, 347, 433, 499, 505
Bsp19I CCATGG 1 cut(s) 509
BspACI CCGC 6 cut(s) 29, 159, 215, 270, 330, 381
BspANI GGCC 3 cut(s) 308, 328, 492
BspLI GGNNCC 3 cut(s) 166, 491, 492
BspPI GGATC 2 cut(s) 355, 500
BspQI GCTCTTC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 164
BsrFI RCCGGY 3 cut(s) 161, 211, 308
BssAI RCCGGY 3 cut(s) 161, 211, 308
BssECI CCNNGG 2 cut(s) 342, 509
BssMI GATC 5 cut(s) 54, 347, 433, 499, 505
BssNI GRCGYC 4 cut(s) 230, 275, 293, 312
BssT1I CCWWGG 1 cut(s) 509
Bst4CI ACNGT 2 cut(s) 204, 301
Bst6I CTCTTC 2 cut(s) 108, 251
BstACI GRCGYC 4 cut(s) 230, 275, 293, 312
BstBAI YACGTR 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 310, 330
BstDSI CCRYGG 1 cut(s) 509
BstF5I GGATG 1 cut(s) 405
BstKTI GATC 5 cut(s) 57, 350, 436, 502, 508
BstMAI GTCTC 2 cut(s) 27, 240
BstMBI GATC 5 cut(s) 54, 347, 433, 499, 505
BstMWI GCNNNNNNNGC 2 cut(s) 267, 489
BstSCI CCNGG 2 cut(s) 341, 492
BstSFI CTRYAG 1 cut(s) 177
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 2 cut(s) 20, 254
BstV2I GAAGAC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 3 cut(s) 308, 328, 492
BtgI CCRYGG 1 cut(s) 509
BtsCI GGATG 1 cut(s) 405
Cac8I GCNNGC 2 cut(s) 310, 330
Cfr10I RCCGGY 3 cut(s) 161, 211, 308
Cfr13I GGNCC 3 cut(s) 327, 490, 491
CseI GACGC 4 cut(s) 238, 264, 282, 301
Csp6I GTAC 2 cut(s) 165, 369
CviAII CATG 3 cut(s) 133, 437, 510
CviQI GTAC 2 cut(s) 165, 369
DpnI GATC 5 cut(s) 56, 349, 435, 501, 507
DpnII GATC 5 cut(s) 54, 347, 433, 499, 505
DriI GACNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 306
Eam1104I CTCTTC 2 cut(s) 108, 251
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 2 cut(s) 108, 251
EciI GGCGGA 1 cut(s) 18
Ecl136II GAGCTC 3 cut(s) 76, 88, 142
Eco130I CCWWGG 1 cut(s) 509
Eco24I GRGCYC 4 cut(s) 78, 90, 144, 494
Eco53kI GAGCTC 3 cut(s) 76, 88, 142
Eco72I CACGTG 1 cut(s) 486
EcoICRI GAGCTC 3 cut(s) 76, 88, 142
EcoO109I RGGNCCY 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 509
EcoT38I GRGCYC 4 cut(s) 78, 90, 144, 494
ErhI CCWWGG 1 cut(s) 509
Esp3I CGTCTC 1 cut(s) 240
FaeI CATG 3 cut(s) 136, 440, 513
FaiI YATR 3 cut(s) 134, 438, 511
FatI CATG 3 cut(s) 132, 436, 509
FauI CCCGC 3 cut(s) 263, 337, 374
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 9, 12, 216, 268
FokI GGATG 1 cut(s) 412
FriOI GRGCYC 4 cut(s) 78, 90, 144, 494
Fsp4HI GCNGC 4 cut(s) 9, 12, 216, 268
GluI GCNGC 4 cut(s) 9, 12, 216, 268
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 3 cut(s) 308, 328, 492
HgaI GACGC 4 cut(s) 238, 264, 282, 301
Hin1I GRCGYC 4 cut(s) 230, 275, 293, 312
Hin1II CATG 3 cut(s) 136, 440, 513
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 3 cut(s) 35, 100, 287
HphI GGTGA 1 cut(s) 201
Hpy166II GTNNAC 2 cut(s) 297, 369
Hpy188I TCNGA 4 cut(s) 190, 228, 286, 518
Hpy188III TCNNGA 3 cut(s) 52, 247, 503
Hpy8I GTNNAC 2 cut(s) 297, 369
Hpy99I CGWCG 3 cut(s) 232, 280, 317
HpyAV CCTTC 2 cut(s) 406, 410
HpyCH4III ACNGT 2 cut(s) 204, 301
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 3 cut(s) 136, 195, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 489
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 4 cut(s) 230, 275, 293, 312
Hsp92II CATG 3 cut(s) 136, 440, 513
KpnI GGTACC 1 cut(s) 168
KroI GCCGGC 1 cut(s) 308
KroNI GCCGGC 1 cut(s) 310
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 5 cut(s) 54, 347, 433, 499, 505
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 2 cut(s) 20, 254
MaeII ACGT 1 cut(s) 485
MalI GATC 5 cut(s) 56, 349, 435, 501, 507
MboI GATC 5 cut(s) 54, 347, 433, 499, 505
MboII GAAGA 3 cut(s) 125, 198, 268
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 5 cut(s) 78, 90, 144, 485, 494
MlyI GAGTC 2 cut(s) 44, 281
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 4 cut(s) 36, 118, 121, 439
MroNI GCCGGC 1 cut(s) 308
MseI TTAA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 11
MspR9I CCNGG 2 cut(s) 343, 494
Mva1269I GAATGC 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 267, 489
NaeI GCCGGC 1 cut(s) 310
NciI CCSGG 2 cut(s) 343, 494
NcoI CCATGG 1 cut(s) 509
NdeII GATC 5 cut(s) 54, 347, 433, 499, 505
NgoMIV GCCGGC 1 cut(s) 308
NlaIII CATG 3 cut(s) 136, 440, 513
NlaIV GGNNCC 3 cut(s) 166, 491, 492
NmeAIII GCCGAG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 251
PctI GAATGC 1 cut(s) 383
PdiI GCCGGC 1 cut(s) 310
PfeI GAWTC 1 cut(s) 100
PflFI GACNNNGTC 1 cut(s) 232
PkrI GCNGC 4 cut(s) 10, 13, 217, 269
PleI GAGTC 2 cut(s) 43, 281
PmaCI CACGTG 1 cut(s) 486
PmlI CACGTG 1 cut(s) 486
PpsI GAGTC 2 cut(s) 43, 281
Ppu21I YACGTR 1 cut(s) 486
Psp124BI GAGCTC 3 cut(s) 78, 90, 144
PspCI CACGTG 1 cut(s) 486
PspN4I GGNNCC 3 cut(s) 166, 491, 492
PspOMI GGGCCC 1 cut(s) 490
PspPI GGNCC 3 cut(s) 327, 490, 491
PsuI RGATCY 1 cut(s) 499
PsyI GACNNNGTC 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 166, 370
RsaNI GTAC 2 cut(s) 165, 369
SacI GAGCTC 3 cut(s) 78, 90, 144
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 4 cut(s) 9, 12, 216, 268
Sau3AI GATC 5 cut(s) 54, 347, 433, 499, 505
Sau96I GGNCC 3 cut(s) 327, 490, 491
SchI GAGTC 2 cut(s) 44, 281
ScrFI CCNGG 2 cut(s) 343, 494
SduI GDGCHC 5 cut(s) 78, 90, 144, 485, 494
SetI ASST 9 cut(s) 13, 78, 90, 132, 144, 184, 269, 450, 488
SfcI CTRYAG 1 cut(s) 177
SgrAI CRCCGGYG 1 cut(s) 211
SsiI CCGC 6 cut(s) 29, 159, 215, 270, 330, 381
SstI GAGCTC 3 cut(s) 78, 90, 144
StyD4I CCNGG 2 cut(s) 341, 492
StyI CCWWGG 1 cut(s) 509
TaaI ACNGT 2 cut(s) 204, 301
TaiI ACGT 1 cut(s) 488
TaqI TCGA 1 cut(s) 315
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseI GCWGC 3 cut(s) 8, 11, 267
TspDTI ATGAA 1 cut(s) 471
TspGWI ACGGA 1 cut(s) 37
Tth111I GACNNNGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.