MD08G1069400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016386
Physical Location & Seq
Reverse (-)
5507522 .. 5509182
1661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1069400.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGGAAGCAGCTTCTGCACTCTGAAGAGGAAAAATATGGCAGCTGTTGCAACGACGCTGGTTTGCGTGAAGCAAGTGAAGCAAGAGGCGTCGGAAGAGTGGGACGAGAGCATGCCATTGCCCGGGGACATCATTGAAGGGGTTTATAACTCCGAGAGCCGTGACAATATTGGTGACGAGCTTTTCGTGCCTACGAAAGCCAAGGCAGAGCTTACGTCGCTGCTCGCTAAGTTCAGCAATGTGGATGTCATTTGGGTGAAGGTGAGGAGGGGTGATAGCACTCTCAAGCTCCGGACGGGCGTTGTGGCTGAGAGGCTTTCGATGCTTCACAAAAAGTATACGATCAAAGCGGCTACGGATGATAGACATGTTGCAGTTTTGGGGGACTTGACCTCGGATCAATTCACTGAGCTGCAAGAAATGAGCAGGAGTTTGGTGAATGTGGAATACAGGGGATACAACAAAAAGGGAGTTAAATACGACTGGAAGATGAAGGTCGACAGCTACTTGCCCGACCAACGGTGCACGATCGTTAGCTCCATTCTGTTCATGCCGCTGCCAGGCGAGTACTGCACGGAGGCCACAACTGCCCGGTGCATGGCTTGGTTTTCTGCTGCAGTTGCTTCTGGAGCCCCGCTTATTTTTGTTAACATCCAAACAGAACAGATTGTGTCGTCGGGCTTGATTTGCATACACTCGGTAGCAAAAGGTTCGCCCGCAGATCGATCAGGGCTTAGAAGCCTTCATGAGGAAGCATATGCAAGTGGTTACCAGCTTGTGATATCCAGGTTAGAAGGCAAGAGCTTGATGCCTTCATATGTCAGCTCCGCCGGGCTCATACATTGCTGTGATCACACCGAAATTAAAGAGACTCTTCACTCGGCGATCGATCAGATGGACAGAGTTCAGTTCCACCTCATGGCCTGGTCTAACCATTTGCTTCGAGTTGCTCCGAAATCTGTTGGTGTCGCTACGCTCCGGCCGCCGAGCGGATGCTGTCCCCCAAGAACAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

37.37

Weight (kDa)

7.99

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016386)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g08721
malus_domestica MD08G1069400.v1.1 MD15G1052700.v1.1
prunus_persica Prupe.1G406600_v2.0.a1
pyrus_communis pycom08g05720
rosa_chinensis RchiOBHm_Chr1g0343961
rosa_laevigata RLG00000028943
rosa_multiflora Rmu_ssc0000434.1_g000040
rosa_roxburghii Rroxscaffold_4G00310210
rosa_rugosa Rorug01G0170000
rosa_samantha Rh1AG186600 Rh1BG153000 Rh1CG172000 Rh1DG185300
rosa_wichuraiana Rw1G015390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 919
AccBSI CCGCTC 1 cut(s) 990
AccI GTMKAC 2 cut(s) 338, 498
AccIII TCCGGA 1 cut(s) 291
AciI CCGC 7 cut(s) 350, 554, 635, 717, 828, 983, 990
AclWI GGATC 1 cut(s) 405
AcoI YGGCCR 1 cut(s) 980
AcuI CTGAAG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 89
AfaI GTAC 1 cut(s) 569
AfiI CCNNNNNNNGG 7 cut(s) 122, 519, 560, 598, 748, 919, 989
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 3 cut(s) 559, 785, 923
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 1 cut(s) 863
Alw44I GTGCAC 1 cut(s) 523
AlwI GGATC 1 cut(s) 405
AlwNI CAGNNNCTG 1 cut(s) 15
Ama87I CYCGRG 1 cut(s) 122
Aor13HI TCCGGA 1 cut(s) 291
AoxI GGCC 3 cut(s) 579, 921, 980
ApaLI GTGCAC 1 cut(s) 523
ApeKI GCWGC 6 cut(s) 9, 41, 220, 412, 556, 614
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AsuC2I CCSGG 4 cut(s) 123, 124, 592, 832
AsuHPI GGTGA 5 cut(s) 185, 268, 274, 284, 448
AvaI CYCGRG 1 cut(s) 122
BaeGI GKGCMC 1 cut(s) 527
BanII GRGCYC 2 cut(s) 634, 837
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 6 cut(s) 21, 53, 207, 399, 543, 601
BccI CCATC 1 cut(s) 889
BceAI ACGGC 1 cut(s) 144
BcgI CGANNNNNNTGC 2 cut(s) 693, 727
BciT130I CCWGG 3 cut(s) 561, 787, 925
BciVI GTATCC 1 cut(s) 449
BclI TGATCA 1 cut(s) 850
BcnI CCSGG 4 cut(s) 123, 124, 592, 832
BcoDI GTCTC 1 cut(s) 863
BfmI CTRYAG 1 cut(s) 615
BfuI GTATCC 1 cut(s) 449
BisI GCNGC 9 cut(s) 10, 42, 221, 351, 413, 554, 557, 615, 983
BlsI GCNGC 9 cut(s) 11, 43, 222, 352, 414, 555, 558, 616, 984
BmcAI AGTACT 1 cut(s) 569
Bme1390I CCNGG 7 cut(s) 123, 124, 561, 592, 787, 832, 925
BmeT110I CYCGRG 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 631
BmrFI CCNGG 7 cut(s) 123, 124, 561, 592, 787, 832, 925
BmsI GCATC 3 cut(s) 312, 798, 983
BpmI CTGGAG 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 4 cut(s) 123, 124, 592, 832
Bsa29I ATCGAT 2 cut(s) 724, 888
BsaHI GRCGYC 1 cut(s) 89
BsaJI CCNNGG 4 cut(s) 122, 123, 201, 393
BsaWI WCCGGW 1 cut(s) 291
Bsc4I CCNNNNNNNGG 7 cut(s) 122, 519, 560, 598, 748, 919, 989
Bse1I ACTGG 1 cut(s) 488
Bse3DI GCAATG 3 cut(s) 116, 244, 841
BseAI TCCGGA 1 cut(s) 291
BseBI CCWGG 3 cut(s) 561, 787, 925
BseCI ATCGAT 2 cut(s) 724, 888
BseDI CCNNGG 4 cut(s) 122, 123, 201, 393
BseGI GGATG 4 cut(s) 250, 364, 651, 998
BseLI CCNNNNNNNGG 7 cut(s) 122, 519, 560, 598, 748, 919, 989
BseMI GCAATG 3 cut(s) 116, 244, 841
BseMII CTCAG 2 cut(s) 300, 399
BseNI ACTGG 1 cut(s) 488
BseRI GAGGAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 527
BseX3I CGGCCG 1 cut(s) 980
BseXI GCAGC 6 cut(s) 21, 53, 207, 399, 543, 601
BsgI GTGCAG 1 cut(s) 556
Bsh1285I CGRYCG 3 cut(s) 531, 888, 983
BshFI GGCC 3 cut(s) 581, 923, 982
BshVI ATCGAT 2 cut(s) 724, 888
BsiEI CGRYCG 3 cut(s) 531, 888, 983
BsiHKAI GWGCWC 1 cut(s) 527
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 5 cut(s) 123, 292, 592, 831, 979
BslFI GGGAC 4 cut(s) 116, 140, 398, 984
BslI CCNNNNNNNGG 7 cut(s) 122, 519, 560, 598, 748, 919, 989
BsmAI GTCTC 1 cut(s) 863
BsmFI GGGAC 4 cut(s) 116, 140, 398, 984
BsnI GGCC 3 cut(s) 581, 923, 982
BsoBI CYCGRG 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 527, 634, 837
Bsp13I TCCGGA 1 cut(s) 291
Bsp143I GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
BspACI CCGC 7 cut(s) 350, 554, 635, 717, 828, 983, 990
BspANI GGCC 3 cut(s) 581, 923, 982
BspCNI CTCAG 2 cut(s) 301, 400
BspDI ATCGAT 2 cut(s) 724, 888
BspEI TCCGGA 1 cut(s) 291
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 1 cut(s) 631
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 1 cut(s) 405
BsrBI CCGCTC 1 cut(s) 990
BsrDI GCAATG 3 cut(s) 116, 244, 841
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 4 cut(s) 122, 123, 201, 393
BssMI GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
BssNAI GTATAC 1 cut(s) 339
BssNI GRCGYC 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 339
Bst2UI CCWGG 3 cut(s) 561, 787, 925
Bst4CI ACNGT 1 cut(s) 522
Bst6I CTCTTC 3 cut(s) 20, 90, 879
BstACI GRCGYC 1 cut(s) 89
BstAPI GCANNNNNTGC 2 cut(s) 15, 47
BstC8I GCNNGC 3 cut(s) 113, 225, 717
BstDEI CTNAG 4 cut(s) 228, 309, 408, 734
BstEII GGTNACC 1 cut(s) 767
BstENI CCTNNNNNAGG 1 cut(s) 746
BstF5I GGATG 4 cut(s) 250, 364, 651, 998
BstKTI GATC 8 cut(s) 345, 400, 531, 724, 728, 853, 888, 892
BstMAI GTCTC 1 cut(s) 863
BstMBI GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
BstMCI CGRYCG 3 cut(s) 531, 888, 983
BstNI CCWGG 3 cut(s) 561, 787, 925
BstNSI RCATGY 2 cut(s) 115, 371
BstPI GGTNACC 1 cut(s) 767
BstSCI CCNGG 7 cut(s) 121, 122, 559, 590, 785, 830, 923
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 1 cut(s) 527
BstV1I GCAGC 6 cut(s) 21, 53, 207, 399, 543, 601
BstZ17I GTATAC 1 cut(s) 339
BstZI CGGCCG 1 cut(s) 980
Bsu15I ATCGAT 2 cut(s) 724, 888
BsuI GTATCC 1 cut(s) 449
BsuRI GGCC 3 cut(s) 581, 923, 982
BsuTUI ATCGAT 2 cut(s) 724, 888
BtsCI GGATG 4 cut(s) 250, 364, 651, 998
BtsIMutI CAGTG 1 cut(s) 405
Cac8I GCNNGC 3 cut(s) 113, 225, 717
CaiI CAGNNNCTG 1 cut(s) 15
CciI TCATGA 1 cut(s) 745
Cfr9I CCCGGG 1 cut(s) 122
ClaI ATCGAT 2 cut(s) 724, 888
CseI GACGC 2 cut(s) 64, 78
Csp6I GTAC 1 cut(s) 568
CviAII CATG 6 cut(s) 112, 368, 550, 598, 746, 919
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 4 cut(s) 228, 309, 408, 734
DpnI GATC 8 cut(s) 344, 399, 530, 723, 727, 852, 887, 891
DpnII GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
EaeI YGGCCR 1 cut(s) 980
EagI CGGCCG 1 cut(s) 980
Eam1104I CTCTTC 3 cut(s) 20, 90, 879
EarI CTCTTC 3 cut(s) 20, 90, 879
EciI GGCGGA 1 cut(s) 817
EclXI CGGCCG 1 cut(s) 980
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 2 cut(s) 634, 837
Eco32I GATATC 1 cut(s) 783
Eco52I CGGCCG 1 cut(s) 980
Eco57I CTGAAG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 122
Eco91I GGTNACC 1 cut(s) 767
EcoNI CCTNNNNNAGG 1 cut(s) 746
EcoO65I GGTNACC 1 cut(s) 767
EcoRII CCWGG 3 cut(s) 559, 785, 923
EcoRV GATATC 1 cut(s) 783
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 634, 837
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 6 cut(s) 115, 371, 553, 601, 749, 922
FalI AAGNNNNNCTT 2 cut(s) 858, 890
FaqI GGGAC 4 cut(s) 116, 140, 398, 984
FatI CATG 6 cut(s) 111, 367, 549, 597, 745, 918
FauI CCCGC 2 cut(s) 642, 724
FauNDI CATATG 2 cut(s) 757, 817
FbaI TGATCA 1 cut(s) 850
FblI GTMKAC 2 cut(s) 338, 498
Fnu4HI GCNGC 9 cut(s) 10, 42, 221, 351, 413, 554, 557, 615, 983
FokI GGATG 4 cut(s) 257, 371, 638, 1005
FriOI GRGCYC 2 cut(s) 634, 837
Fsp4HI GCNGC 9 cut(s) 10, 42, 221, 351, 413, 554, 557, 615, 983
GluI GCNGC 9 cut(s) 10, 42, 221, 351, 413, 554, 557, 615, 983
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 3 cut(s) 581, 923, 982
HapII CCGG 5 cut(s) 123, 292, 592, 831, 979
HgaI GACGC 2 cut(s) 64, 78
Hin1I GRCGYC 1 cut(s) 89
Hin1II CATG 6 cut(s) 115, 371, 553, 601, 749, 922
HincII GTYRAC 2 cut(s) 499, 649
HindII GTYRAC 2 cut(s) 499, 649
HinfI GANTC 1 cut(s) 871
HpaI GTTAAC 1 cut(s) 649
HpaII CCGG 5 cut(s) 123, 292, 592, 831, 979
HphI GGTGA 5 cut(s) 185, 268, 274, 284, 448
Hpy166II GTNNAC 4 cut(s) 339, 499, 525, 649
Hpy188I TCNGA 6 cut(s) 24, 94, 154, 397, 894, 954
Hpy188III TCNNGA 3 cut(s) 292, 627, 746
Hpy8I GTNNAC 4 cut(s) 339, 499, 525, 649
Hpy99I CGWCG 4 cut(s) 58, 94, 220, 679
HpyAV CCTTC 6 cut(s) 131, 253, 487, 752, 788, 822
HpyCH4III ACNGT 1 cut(s) 522
HpyCH4IV ACGT 1 cut(s) 215
HpyF3I CTNAG 4 cut(s) 228, 309, 408, 734
HpySE526I ACGT 1 cut(s) 215
Hsp92I GRCGYC 1 cut(s) 89
Hsp92II CATG 6 cut(s) 115, 371, 553, 601, 749, 922
Kpn2I TCCGGA 1 cut(s) 291
Ksp22I TGATCA 1 cut(s) 850
KspAI GTTAAC 1 cut(s) 649
Kzo9I GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
LmnI GCTCC 6 cut(s) 294, 542, 629, 830, 955, 981
Lsp1109I GCAGC 6 cut(s) 21, 53, 207, 399, 543, 601
LweI GCATC 3 cut(s) 312, 798, 983
MaeII ACGT 1 cut(s) 215
MaeIII GTNAC 3 cut(s) 161, 173, 767
MalI GATC 8 cut(s) 344, 399, 530, 723, 727, 852, 887, 891
MbiI CCGCTC 1 cut(s) 990
MboI GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
MboII GAAGA 4 cut(s) 37, 107, 499, 866
MhlI GDGCHC 3 cut(s) 527, 634, 837
MluCI AATT 2 cut(s) 401, 861
MlyI GAGTC 1 cut(s) 865
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 9 cut(s) 21, 79, 258, 261, 306, 403, 571, 742, 926
MroI TCCGGA 1 cut(s) 291
MseI TTAA 3 cut(s) 474, 648, 864
MslI CAYNNNNRTG 2 cut(s) 254, 846
MspA1I CMGCKG 2 cut(s) 44, 556
MspI CCGG 5 cut(s) 123, 292, 592, 831, 979
MspR9I CCNGG 7 cut(s) 123, 124, 561, 592, 787, 832, 925
MvaI CCWGG 3 cut(s) 561, 787, 925
NciI CCSGG 4 cut(s) 123, 124, 592, 832
NdeI CATATG 2 cut(s) 757, 817
NdeII GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
NlaIII CATG 6 cut(s) 115, 371, 553, 601, 749, 922
NlaIV GGNNCC 1 cut(s) 631
NmeAIII GCCGAG 2 cut(s) 860, 1011
NmuCI GTSAC 2 cut(s) 161, 173
NspI RCATGY 2 cut(s) 115, 371
PaeI GCATGC 1 cut(s) 115
PagI TCATGA 1 cut(s) 745
PciI ACATGT 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PflMI CCANNNNNTGG 1 cut(s) 919
PkrI GCNGC 9 cut(s) 11, 43, 222, 352, 414, 555, 558, 616, 984
Ple19I CGATCG 2 cut(s) 531, 888
PleI GAGTC 1 cut(s) 865
PpsI GAGTC 1 cut(s) 865
PscI ACATGT 1 cut(s) 367
PsiI TTATAA 1 cut(s) 147
Psp6I CCWGG 3 cut(s) 559, 785, 923
PspEI GGTNACC 1 cut(s) 767
PspGI CCWGG 3 cut(s) 559, 785, 923
PspN4I GGNNCC 1 cut(s) 631
PstI CTGCAG 1 cut(s) 619
PstNI CAGNNNCTG 1 cut(s) 15
PvuI CGATCG 2 cut(s) 531, 888
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
RseI CAYNNNNRTG 2 cut(s) 254, 846
SalI GTCGAC 1 cut(s) 497
SaqAI TTAA 3 cut(s) 474, 648, 864
SatI GCNGC 9 cut(s) 10, 42, 221, 351, 413, 554, 557, 615, 983
Sau3AI GATC 8 cut(s) 342, 397, 528, 721, 725, 850, 885, 889
ScaI AGTACT 1 cut(s) 569
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 7 cut(s) 123, 124, 561, 592, 787, 832, 925
SduI GDGCHC 3 cut(s) 527, 634, 837
SfaNI GCATC 3 cut(s) 312, 798, 983
SfcI CTRYAG 1 cut(s) 615
SmaI CCCGGG 1 cut(s) 124
SmiMI CAYNNNNRTG 2 cut(s) 254, 846
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SphI GCATGC 1 cut(s) 115
Sse9I AATT 2 cut(s) 401, 861
SsiI CCGC 7 cut(s) 350, 554, 635, 717, 828, 983, 990
SspI AATATT 1 cut(s) 169
StyD4I CCNGG 7 cut(s) 121, 122, 559, 590, 785, 830, 923
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 522
TaiI ACGT 1 cut(s) 218
TaqI TCGA 5 cut(s) 320, 498, 724, 888, 943
TasI AATT 2 cut(s) 401, 861
TatI WGTACW 1 cut(s) 567
TauI GCSGC 3 cut(s) 353, 556, 985
Tru1I TTAA 3 cut(s) 474, 648, 864
Tru9I TTAA 3 cut(s) 474, 648, 864
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 2 cut(s) 161, 173
TseI GCWGC 6 cut(s) 9, 41, 220, 412, 556, 614
Tsp45I GTSAC 2 cut(s) 161, 173
TspDTI ATGAA 4 cut(s) 506, 538, 734, 804
TspGWI ACGGA 2 cut(s) 371, 590
TspMI CCCGGG 1 cut(s) 122
TspRI CASTG 1 cut(s) 412
Van91I CCANNNNNTGG 1 cut(s) 919
VneI GTGCAC 1 cut(s) 523
XagI CCTNNNNNAGG 1 cut(s) 746
XceI RCATGY 2 cut(s) 115, 371
XmaI CCCGGG 1 cut(s) 122
XmiI GTMKAC 2 cut(s) 338, 498
ZrmI AGTACT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.